DEAD END FILTRATION

DESIGN OF A FILTRATION SYSTEM FOR BIOSYNTHETIC INSULIN PRODUCTION

CHEE 450March 30, 2009

Jeff DeMezaBlair Walker

Richard Wong

Dead End Filtration Theory

Removes particulates larger than a specified pore size from filtrate

Particulates accumulate as a filter cake

Batch operation Flow properties

change with time› Filtrate flux› Pressure drop

Dead End Filtration TheoryDepth Filter

Process Overview Removes residual IBs, cell debris &

biomass Specifications

› 1.25 hours filtration time, 23.3 kg filter cake/batch

Design Consideration

3mm

+ 100% Safety FactorastePermeateFilter

A filter surface = 13.8 m2

Determine Filter Area

Design Considerations

Through Put Pressure Disposable

Other considerations

Q = 1746 L/h

A = 13.8m2

Pore Size = 0.2 μm

Contact Venders &

Product Information

Combined sterile filter and casing design

Dispose after each batch

Most expensive filter Validation

requirements are met Low capital costs

Design ConsiderationsDisposable Filter and Casing

Disposable filter Reusable Stainless

steel casing No contact of filtrate

with casing Casing does not need

to be cleaned between batches

Initial validation required

Large initial capital cost of casing

Design ConsiderationsReusable Stainless Steel Casing

Economic AnalysisSteel Casing

and Disposable

Filter

Disposable Filter and

Casing

Capital Cost $50 000 ~$0

Cost of filter per batch $2 500 $3 900

Total cost for year 1

$450 000 $624 000

Economic Analysis

1 2 3 4 50

500

1000

1500

2000

2500

3000

3500

4000

Multi-useDisposable

Year

Batc

h C

ost

$

Note: does not include validation costs

Recommended Filter

Produced by Pall> ~ $2500 / batch> Area surface = 14m2

> P = 3.5 bar> Pore size = 0.2

Quattro Flow pump manufactured by Triangle Process Equipment

Reusable Stainless Steel Casing with Disposable Filter

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Questions?