charles louischarles louis davis dvmdavis dvm ... sheep and goats.pdf-autosomal recessive, lysosomal...
TRANSCRIPT
![Page 1: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/1.jpg)
CHARLES LOUISCHARLES LOUISDAVIS DVMDAVIS DVMFOUNDATIONFOUNDATIONTOPICS IN LABORATORY ANIMAL MEDICINETOPICS IN LABORATORY ANIMAL MEDICINEMay 18, 2007May 18, 2007
SHEEP AND GOATSSHEEP AND GOATS
Melaney K. Gee, MS, DVMMelaney K. Gee, MS, DVMWake Forest University Health SciencesWake Forest University Health Sciences
![Page 2: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/2.jpg)
THANK YOU!THANK YOU!
Slides and accompanying information providedSlides and accompanying information providedby Diane Forsythe and Mary Grant ofby Diane Forsythe and Mary Grant ofNIEHS/NIH, RTP, North Carolina and SueNIEHS/NIH, RTP, North Carolina and SueSpray of the Scripps Research InstituteSpray of the Scripps Research Institute
We wish to extend appreciation to those whoWe wish to extend appreciation to those whocontributed to this collection. The contributorscontributed to this collection. The contributorsare many and without their assistance thisare many and without their assistance thiscollection would not be possiblecollection would not be possible
![Page 3: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/3.jpg)
DisclaimersDisclaimers
This is not an ACLAM sanctioned presentationThis is not an ACLAM sanctioned presentation
Every attempt to ensure information is reliableEvery attempt to ensure information is reliableand correct (but I am not an expert)and correct (but I am not an expert)
Grain of salt?Grain of salt?
No information presented is known to beNo information presented is known to bespecifically included in ACLAM Boardspecifically included in ACLAM Boardexaminationsexaminations
![Page 4: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/4.jpg)
RuminantsRuminantsSheep and goatsSheep and goats
![Page 5: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/5.jpg)
Genus and species:
Order:
Family:
Capra hircus
Artiodactyla
Bovidae
Male:
Castrated Male:
Female:
Young:
Buck (billy)
Wether
Doe (nanny)
kid
Breed: toggenburg
![Page 6: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/6.jpg)
Breed: Saanen
Location of scentglands:
Behind horn bud
![Page 7: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/7.jpg)
Breed: La Mancha
Polled trait:
Dominant allele,which is linked tothe intersex trait
Goats havesmallest RBC’sand lowest PCV
Other tidbits:
![Page 8: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/8.jpg)
Breed: Nubian
Model for which disease:
Beta mannosidosis -
-Autosomal recessive, lysosomalstorage disease
-Accumulation of mannose(oligosaccharides) due to lack oflysosomal hydroxylase enzyme
-Results in a neurologic disorder,paucity of white matter, myelinand neuronal vacuolation
![Page 9: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/9.jpg)
Model for what disease:
Autosomal dominant trait
Transient spasms of skeletalmuscles brought about by visual,tactile or auditory stimuli
Caused by mutation in a generesponsible for down regulatingelectrical excitation in the muscles
Myotonia congenita
“Thomson’s Disease”“Fainting Goats”
![Page 10: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/10.jpg)
SheepSheepGenus and species: Ovis aries
Male:
Castrated male:
Female:
Young:
Ram
Wether
Ewe
Lamb
Location of Sebaceous Glands:
Below eye and between toes
![Page 11: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/11.jpg)
Breed: Suffolk
•Pruritis
•Nervous
•Excitable
•Tremors
![Page 12: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/12.jpg)
Diagnosis: Scrapie
• Neuron - large cytoplasmicvacuoles (spongiform)
• Medulla oblongata, pons andmidbrain
• Prion disease
• Transmissable spongiformencephalopathy
• Gentetic component
•Suffolk are susceptble
•Targees are resistant
• Specific codon/genes identified
•171- genes Q, R or H
•136- genes A or V
•R and A confer resistance
![Page 13: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/13.jpg)
Collection of the third eyelidlymphoid tissue to detectScrapie
Immunohistochemicaldetection of PrP whichaccumulates in thelymphatic tissue of theinner eyelid of sheep
Test is positive one yearbefore clinical signsdevelop (Vet Forum;June 1998;April98, NewScientist)
What procedure is being performed?
![Page 14: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/14.jpg)
Other SpongiformOther Spongiform EncephalopathiesEncephalopathies
ScrapieScrapie –– sheep and goatssheep and goats
BovineBovine SpongiformeSpongiforme EncephalopathyEncephalopathy -- bovinebovine
Transmissible Mink EncephalopathyTransmissible Mink Encephalopathy -- minkmink
Chronic Wasting DiseaseChronic Wasting Disease of deer and elkof deer and elk
Feline spongiform encephalopathyFeline spongiform encephalopathy
KuruKuru -- humanhuman
CruetzfieldCruetzfield--JakobJakob DiseaseDisease -- humanhuman
GerstmannGerstmann--StrausslerStraussler –– humanhuman
![Page 15: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/15.jpg)
Submandibular edema, with abscesses, draining tracts and granulomas
Diagnosis: Rule out:“Wooden tongue”
Actinobacillus lignieresii
Gram negative rod
Goats- not affectedSheep – lipCattle – tongue
“Lumpy Jaw”
Arcanobacterium pyogenes, A. bovis
Gram positive rod/coccobacillis
Affects bone
Rare in sheep and goats
![Page 16: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/16.jpg)
![Page 17: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/17.jpg)
Diagnosis: Contagious ecthyma (Orf, sore mouth)-Parapoxvirus
-Primary lesions on lips and mouth
-Usually seen in animals < 1yr
-High morbidity/Low mortalityRule outs:
Blue tongue-orbivirus-cyanosis, ulcers of the dental pad,gingiva and tongue, chorioretinitis,conjunctivitis, coronitis
Ulcerative dermatosis-poxvirus-ulcers of face, genitals and feet
Foot and Mouth Disease-picornavirus-vesicles around the mouth, hoovesand teats
Vesicular Stomatitis-rhabdovirus-vesicles on the oral mucusmembranes, teats, interdigital spaces
![Page 18: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/18.jpg)
ContagiousContagious ecthymaecthyma ZoonoticZoonotic diseasedisease
humanhuman--toto--human transmissionhuman transmissioncan occurcan occur
Handlers should wear PPEHandlers should wear PPE
Disinfect clippers, earDisinfect clippers, ear taggerstaggers etcetcbetween infected animalsbetween infected animals
Commercial vaccine available,Commercial vaccine available,but should be used with caution,but should be used with caution,may induce lesions in handlersmay induce lesions in handlers
Do not vaccinate herds that areDo not vaccinate herds that arealready free of the diseasealready free of the disease
![Page 19: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/19.jpg)
![Page 20: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/20.jpg)
Diagnosis:
Haemonchus Haemonchus Haemonchus
Haemonchus contortus-barbor pole worm
![Page 21: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/21.jpg)
Clinical signsClinical signs-- Pallor, severe anemiaPallor, severe anemia
SubmandibularSubmandibular edema (edema (““bottlejawbottlejaw””))-- hypoproteinemiahypoproteinemia
Weight loss,Weight loss, diarhheadiarhhea
UnthriftinessUnthriftiness, decreased milk production, poor wool coat, decreased milk production, poor wool coat
![Page 22: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/22.jpg)
PathogenesisPathogenesis Direct life cycleDirect life cycle Ingestion of larvae to eggs passed in feces occurs in 3wksIngestion of larvae to eggs passed in feces occurs in 3wks
HypobioticHypobiotic (arrested) larvae may exist in host(arrested) larvae may exist in host ““spring risespring rise””-- large number of larvae passed fromlarge number of larvae passed from
peripartuientperipartuient ewes onto pastureewes onto pasture Blood meals from mucosa ofBlood meals from mucosa of abomasumabomasum
Treatment ControlTreatment Control AntihelminthicsAntihelminthics Severe resistance has developed!Severe resistance has developed!
Facility sanitation and pasture management and rotationFacility sanitation and pasture management and rotation Susceptible to freezing and dry conditionsSusceptible to freezing and dry conditions
![Page 23: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/23.jpg)
Tissue from a sheep
Diagnosis: Nodule wormOesophagostomum columbianumOesophagostomum venulosum
![Page 24: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/24.jpg)
Tissue from a sheep
![Page 25: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/25.jpg)
Diagnosis:
Goiter
Congenital Goiter
Merino sheep
Nutritional Goiter
-due to iodine deficiency
-consumption of goitrogenicplants (soybeans, rape, kale,cabbage and turnips)
http://www.pipevet.com/photos/goiter.htmPipestone Vet Clinic
![Page 26: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/26.jpg)
Clinical exam
![Page 27: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/27.jpg)
Necropsy
![Page 28: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/28.jpg)
Necropsy
![Page 29: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/29.jpg)
Blood smear (Wright Geimsa)
Describe 3 morphologicchanges:
Polychromasia (Hb)
Poikilocytosis (shape)
Anisocytosis (size)
Heinz body(curved arrow)
Howell Jolly body(straight arrow)
Describe arrows:
![Page 30: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/30.jpg)
Presumptive Diagnosis:
Copper Toxicosis
- Icterus/hemolysis
- Enlarged black/brownliver/spleen
-“Gun-barrel” black kidneys
-Hematuria/hemoglobinuri
![Page 31: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/31.jpg)
CopperCopper toxicosistoxicosis
• Sheep store Cu readily
• Single toxic dose range = 20-100 mg/kg ( vs 220-880 mg/kg in cattle)
• Cu released from liver is directly toxic to RBC membranes
Pathogenesis:
• D-penicillamine, Mb, thiosulfate, tetrathiomolybdate
Treatment:
Cause:
Sheep fed improperly balanced rations or cattle diets
Feed low in molybdenum, zinc or calcium
Phytogenous sources- subterranean clover-Merino sheep may be more susceptible to thiscause than other breeds
![Page 32: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/32.jpg)
Model for Wilson’s disease
Human genetic defect in copper the transporting pHuman genetic defect in copper the transporting p--typetype ATPaseATPase
NorthernNorthern RonaldsayRonaldsay SheepSheep
Other well known animal model (rodent) for Wilson’s disease?
Long Evans Cinnamon (LEC) Rat
![Page 33: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/33.jpg)
What are the nodules?
Normal caruncles
Describe seasonal estrouscycle for sheep/goats?
Seasonally polyestrous
Fall/Winter Breeders
Estrous: 14-19 d (shp), 18-24d (gt)
Estrus: 24-30hr (shp), 24-96 (gt)
Gestation length: 145-155 d (150d)
Tissue from a sheep
Uterus
Syndesmochorial placenta
Cotyledon: the fetal side of theplacentaCaruncle: the maternal side of theplacentaPlacentome: a cotyledon andcaruncle together
![Page 34: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/34.jpg)
Sheep – subcutaneous surface of the skin
![Page 35: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/35.jpg)
![Page 36: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/36.jpg)
Presumptive diagnosis?
Bovine Leukemia Virus
B lymphocyte associatedretrovirus
Are leukemic (rare) as well assolid tumors (common)
Common sites – lymphoidtissue, abomasum, spinalcanal, retrobulbar, uterus andother abdominal organs
Goats seroconvert but do notdevelop clinical disease
Lymphosarcoma
![Page 37: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/37.jpg)
Tissue from a lamb-Dyspnea, paresis, stiffness,inability to stand
-Other lambs found dead
-Creatinine Kinase (CK) andaspartate aminotransferase(AST) elevated
(aka: stiff lamb disease, nutritionalmyodegeneration, nutritional musculardystrophy)
Clinical signs:
Presumptive diagnosis?
White Muscle Disease
Pathogenesis
-Vitamin E and/or Selenium deficiency-Lack of one or both results in oxidativestress and loss of membrane integrity
-Cardiac form -neonates-Skeletal form- young
![Page 38: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/38.jpg)
White Muscle DiseaseWhite Muscle DiseaseDiagnosis
-clinical signs-whole blood levels of vitamin E-plasma levels of selenium-glutathione peroxidase levels inred blood cells
Treatment and Prevention
-Evaluation or awareness of regional soil content-Supplementation of affected animals or late stagegestational ewes with Vitamin E or Selenium injections-Properly balanced dietary rations
*be cautious of selenium “toxicity”
![Page 39: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/39.jpg)
Tissue from a sheep
Presumptive Diagnosis?
Lungworms
Dictyocaulus filaria*
Protostrongylus rufescens
Muellarius capillaris
Less common in goats
![Page 40: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/40.jpg)
Tissue from a goatTissue from a goat
Listeria monocytogenes
Diagnosis?Diagnosis?
--AbortionAbortion--CNS signsCNS signs
Cold enrichment (20OC) beneficialin culturing the organism
![Page 41: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/41.jpg)
Most likely diagnosis?Most likely diagnosis?
Corynebacterium pseudotuberculosis
-Disseminated superficial abscesses of lymph nodes
- Very common
-Gram + coccobacillus
-Thick caseous exudate
-ELISA available
![Page 42: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/42.jpg)
Goat small intestineGoat small intestine
Young animal with hemorrhagic diarrheaYoung animal with hemorrhagic diarrhea
Presumptive diagnosis?Presumptive diagnosis?
Coccidiosis(Eimeria ninakohlyakimovae,E. arloingi, E. christenseni)
Necropsy Findings:GI may appear congested,hemorrhagic, or ulcerated andhave scattered pale, yellow towhite mucosal plaques
-common in young animals-often associated with stress or intensivehousing conditions, or weaning-11 Eimeria species in sheep, 9 in goats
![Page 43: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/43.jpg)
Brain from a ruminant with CNS diseaseBrain from a ruminant with CNS disease
Histology
-Negri bodies in thecytoplasm of the neuron-Confirmation made byfluorescent antibody stainof the brain
Rabies(Lyssavirus-genus)(Rhabdovirus-family)
Zoonotic!
Reportable!
Diagnosis?
![Page 44: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/44.jpg)
Frontal sinus of a sheepFrontal sinus of a sheep
![Page 45: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/45.jpg)
Name the ParasiteName the Parasite
Oestrus ovisNasal bot fly - larva
![Page 46: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/46.jpg)
Clinical ExamClinical Exam –– fetlock of a goatfetlock of a goat
PruritusPruritus, scales, crusts and hyperkeratosis, scales, crusts and hyperkeratosis
![Page 47: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/47.jpg)
Diagnosis?Diagnosis?
Chorioptes bovis-Affects lower legs and scrotum --Usually occurs in cooler months
Psoroptes cuniculi (ears – rare)
Sarcoptes scabiei (head scabies)
Rule outs?Rule outs?
Scabies in sheep:Psoroptes ovis, Sarcoptes scabiei, Psorergates ovis, Chorioptes ovis
-Ruminant mites have been eradicatedor are very rare in the US
-Sarcoptes and Psorergates infectionsare reportable!
![Page 48: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/48.jpg)
Parasite found on sheep withParasite found on sheep with pruritispruritis,,and chronic dermatitis of the neck,and chronic dermatitis of the neck,sides, abdomen and rumpsides, abdomen and rump
Diagnosis?Diagnosis?
Sheep keds
(Melophagus ovinus)
wingless, flat, brown, bloodsucking fly
Bluetongue
Can transmit which virus?
![Page 49: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/49.jpg)
ModerateModerate gliosisgliosis, non, non--suppurativesuppurativeencephalitis,encephalitis, perivascularperivascular mononuclearmononuclearinfiltratesinfiltrates
www.k-state.edu/.../625tutorials/FIGbrain2.jpg
Brain from aborted goat fetusBrain from aborted goat fetus
![Page 50: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/50.jpg)
Diagnosis?Diagnosis?
NeosporaNeospora caninumcaninum
--Widespread worldwideWidespread worldwide
--Abortion is the only clinical sign in adultsAbortion is the only clinical sign in adults--Young may show weakness or CNSYoung may show weakness or CNSsignssigns
--More common in bovine, but may beMore common in bovine, but may beseen in sheep and goatsseen in sheep and goats
--ImmunohisochemicalImmunohisochemical staining specific forstaining specific forthe organism (the organism (immunoperoxidaseimmunoperoxidase))
ToxoplasmaToxoplasma sp.sp. (smaller)(smaller)
Rule outs?Rule outs?
Definitive host?
Dog
![Page 51: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/51.jpg)
unthriftinessunthriftiness, weight loss and intermittent diarrhea, weight loss and intermittent diarrhea
Tissue from a sheep
![Page 52: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/52.jpg)
HistopathologyHistopathology
![Page 53: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/53.jpg)
Diagnosis?Diagnosis?
Mycobacterium paratuberculosis“Johne’s Disease”
Diagnostic tests?Diagnostic tests?
-Non-spore forming, fastidious,acid-fast, gram-positive rod
-Chronic carriers exist-Most likely route of infection via ingestion-Vertical transmission reported-Organisms inhabit macrophages of host
-Fecal culture: 8-12 weeks-Serology: ELISA (most reliable),
AGID or CF-Acid-fast organisms on rectalbiopsy smears
![Page 54: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/54.jpg)
Name three potentially zoonoticorganisms associated with abortionand/or lambing in sheep:
Coxiella burnetii- Q fever
Brucella ____?____ melitensis
Campylobacter fetus subspecies intestinalis
![Page 55: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/55.jpg)
CoxiellaCoxiella burnetiiburnetii
GramGram--negativenegative coccobacilluscoccobacillus--like bacteria, similar tolike bacteria, similar torickettsialrickettsial organismsorganisms
Found in milk, urine and feces of infected animalsFound in milk, urine and feces of infected animals
Placenta and fetus are particularly dangerous sourcePlacenta and fetus are particularly dangerous sourceof infection for peopleof infection for people
Transmission via inhalation of aerosolized particlesTransmission via inhalation of aerosolized particles
Likely to be asymptomatic in sheepLikely to be asymptomatic in sheep
Causes fluCauses flu--like symptoms in peoplelike symptoms in people
Can be treated with appropriate antibioticsCan be treated with appropriate antibiotics
Q fever
![Page 56: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/56.jpg)
Name the vesicular/ulcerative diseases of sheep and goats?Name the vesicular/ulcerative diseases of sheep and goats?
Bluetongue (Reovirus)
Ulcerative dermatosis (Poxvirus)
Contagious ecthyma (Parapoxvirus)
FMD (Picornavirus)
VS (Rhabdovirus)
![Page 57: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/57.jpg)
Name three respiratory viruses of sheep and goatsName three respiratory viruses of sheep and goats
OPPV (Maedi/Visna – Retrovirus)
Pulmonary Adenomatosis (Jaggsiekte – Type D retrovirus)
CAEV ( older animals – Retrovirus)
Parainfluenza-3 (Paramyxovirus)
RSV (Paramyxovirus)
Adenovirus +/-?
![Page 58: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/58.jpg)
Diseases of sheep and goats that areDiseases of sheep and goats that are zoonoticzoonotic??
Q fever
Contagious ecthyma
Anthrax
Brucellosis
Leptospirosis
Listeriosis
Mycobacterium
Tularemia
Yersinia
Rabies
Cryptosporidium
Toxoplasmosis
Dermatophytes
![Page 59: CHARLES LOUISCHARLES LOUIS DAVIS DVMDAVIS DVM ... Sheep and Goats.pdf-Autosomal recessive, lysosomal storage disease-Accumulation of mannose (oligosaccharides) due to lack of ... Susceptible](https://reader030.vdocuments.us/reader030/viewer/2022011908/5f5b412110bb9767df201d6a/html5/thumbnails/59.jpg)
Next PresentationNext Presentation……