characterization of the suppressive properties of human cd4+cd25+ t cells in atopic and non-atopic...
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397 Clinical Status, Serum Immunoglobulin G Subclasses andSputum Il-10 and Mpo Levels in Cf Patients
A. Korzeniewska, J. Jerzynska, I. Stelmach; Department of Pediatrics
and Allergy, Medical University of Lodz, M Curie Hospital, Zgierz,
POLAND.
RATIONALE: This study was undertaken to define a correlation
between clinical status, serum immunoglobuline G (IgG) subclasses and
sputum IL-10 and MPO levels in patients with CF.
METHODS: We examined 17 clinical stable CF patients aged 12-30. All
patients were chronic infected with Pseudomonas aeruginosa. Shwach-
man-Kulczycki Score (SKS) was measured. Total IgG, IgG1, IgG2, IgG3,
IgG4 (mg/dl) in serum and sputum IL-10 (pg/dl) and MPO (ng/dl) levels
were determined.
RESULTS: Mean SKS was 76±13. Mean total IgG levels were
1510±556.9, IgG1 9.552±2.5l, IgG2 4.8±2.04, IgG3 1.058±0.6 and IgG4
1.787±0.725. Mean sputum IL-10 levels were 10.78±0.76 and MPO
levels 5.01±0.53.
We found significant correlation between elevated total serum IgG levels
and decreased SKS (p=0.002). High levels of IgG2 were significantly cor-
related with decreased SKS (p=0.001). Significant correlation between
IgG2 and sputum MPO (p=0.002) levels was observed; and there was sig-
nificant inverse correlation between IgG2 levels and sputum IL-10
(p=0.001) levels. There was significant correlation between elevated lev-
els of IgG4 and increased SKS (p=0.002) and IL-10 levels (p=0.003). Sig-
nificant inverse correlation between IgG4 levels and sputum (p=0.001)
MPO levels were observed. We found significant correlation between
better SKS and IL-10 levels (p=0.002) and significant inverse correlation
between SKS and sputum MPO levels (p=0.002).
CONCLUSIONS: IgG2 levels showed to be a good value in predicting
the severity of disease and could be a useful marker of inflammatory
process in CF patients chronic infected with Pseudomonas aeruginosa.
Elevated levels of IgG4 may antagonise IgG2 helping to preserve rela-
tively good clinical status.
398 Evaluation of NK T Cells in Patients with Schistosomiasis andAsthma
R. A. Campos, M. S. Teles, M. J. Martins, O. F. Jesus, B. B. Andrade, A.
A. Cruz, M. Araújo, E. M. Carvalho; Internal Medicine, Universidade
Federal da Bahia, Salvador-BA, BRAZIL.
RATIONALE: S. mansoni modulates the immune response in asthma.
V�24V�11 NK T cells release Th1 and Th2 cytokines but there is a pos-
itive correlation between CD4 expression on NKT cells and ability to pro-
duce Th2 cytokines. NKT cell deficient mice infected with S. mansonipresent reduced hepatic pathology. This study evaluated the profile of NK
T cells in asthmatic patients with schistosomiasis.
METHODS: We evaluated 4 groups: 1) controls; 2) asthma; 3) schisto-
somiasis; 4) asthma + schistosomiasis. A questionnaire for asthma was
done and FACS analysis of the PBMC for NKT cells and intracellular
cytokines using PE and FITC anti-V�24, FITC anti-V�11, Cy anti-CD4,
Cy anti-CD3, PE anti-IL-4 and PE anti-IFN-�. NK T cells were defined
by the double expression of V�24 and V�11.
RESULTS: We evaluated 87 subjects: 25 from group 1, 39 from group 2,
12 from group 3 and 11 from group 4. There was a reduction on NKT cells
in asthma (group 1: 0.45%±0.41 and group 2: 0.14%±0.21; p=0.03) but
CD4+NKT cells were higher (group 1: 35%±4.1; group 2: 57%±5.6). There
was no reduction of NKT cells in schistosomiasis and in the asthmatic indi-
viduals with schistosomiasis. The NKT cell intracellular production of IL-4
was similar between groups 3 and 4. Subjects with schistosomiasis and
asthma showed a non significant lower INF-� production.
CONCLUSIONS: In asthma, CD4+ V�24V�11 NKT may be preferen-
tially producing Th2 cytokines and this may be modulated in schistoso-
miasis patients likely by stimulating CD4 - V�24V�11 NKT to produce
Th1 cytokines.
Funding: FAPESB
399 Successful Treatment Using Interleukin-2 (IL-2) in a Patientwith Idiopathic CD4+ Lymphopenia (ICL) and Mycobacteriumavium (M.avium)
B. Mandal1, V. Bhushan2, D. Khan1; 1Allergy and Immunology, UT
Southwestern, Dallas, TX, 2Hematology/Oncology, UT Southwestern,
Dallas, TX.
RATIONALE: ICL is a rare immune defect characterized by: CD4+ T
cells count <300 cells/mm3 on more than one occasion, lack of HIV infec-
tion or other immune deficiency, and presence of opportunistic infections.
Isolated case reports suggest that IL-2 is efficacious in ICL. We report a
case of a patient with M.avium due to ICL who was successfully treated
with IL-2.
METHODS: This patient is a 39 y/o WM who presented with 2 years of
chronic cough and dyspnea. A sputum culture revealed M.avium. His
CD4+ T cells were decreased at 182 cells/�l. Workup for other etiologies
of CD4+ lymphopenia was negative including: HIV-1,2 , HTLV-1,2, chest
CT and bone marrow biopsy. He was diagnosed with ICL and started on
pegylated subcutaneous IL-2 at 500,000 units weekly. He was given
incrementally higher doses until he reached a maintenance dosage 3
months later of 11 MU weekly.
RESULTS: After 3 months of therapy, CD4+ T cells increased to 453
cells/�l. Nine months after initiation of IL-2, his CD4+ T cell counts have
been >450 cells/�l. Sputum cultures became negative for M.avium, and he
has tolerated the IL-2 with no side effects.
CONCLUSIONS: IL-2 therapy appears to be a relatively safe and effec-
tive treatment for patients with ICL and should be considered for select
patients to prevent the high morbidity associated with this syndrome.
Funding: UT Southwestern
400 Characterization of the Suppressive Properties of HumanCD4+CD25+ T Cells in Atopic and Non-Atopic Individuals
A. M. L. Fonseca, C. Torres, O. M. M. Lourenco, L. M. P. Taborda-Barata;
Department of Medical Sciences, Health Sciences Medical Center, Uni-
versity of Beira interior, Covilhã, PORTUGAL.
RATIONALE: The aim of the present study was to analyze whether
“regulatory” CD4+CD25+ T cells are present and function normally in
atopic individuals, namely in terms of the suppression of allergen-specific
proliferation and cytokine production of CD4+CD25 T cells.
METHODS: Peripheral blood was obtained from 12 atopic subjects and
12 non-atopic controls and CD4+CD25+ and CD4+CD25 cells were
isolated using magnetic beads. CD4+CD25+ and CD4+CD25 T cells
were mixed at different ratios (2: 1, 4: 1 and 8: 1) and stimulated with Der
p or PHA in 6-day co-cultures together with autologous monocytes as
antigen-presenting cells. Proliferation and cytokine production was
analysed using thymidine incorporation and cytometric bead array,
respectively.
RESULTS: Our results show that the percentage of CD4+CD25+ T cells
was not significantly different between groups. CD4+CD25+ cells sup-
pressed proliferation by their Der p-stimulated CD4+CD25 T cells in all
studied samples. Moreover, the amount of IFN-�, IL-2, IL-4, IL-5, Il-10,
TNF-� produced by the co-cultures was comparable in both groups
studied.
CONCLUSIONS: These preliminary data indicate that “regulatory”
CD4+CD25+ T cells are equally present and functional in both atopic and
non-atopic individuals.
Funding: Fundacao para a Ciencia e Tecnologia
S102 Abstracts J ALLERGY CLIN IMMUNOL
FEBRUARY 2006
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