chapter 5: microbial metabolism
DESCRIPTION
Chapter 5: Microbial Metabolism. METABOLISM. Sum of all chemical rxn’s within a living organism Release energy Require energy. CATABOLIC REACTION (breakdown) Breakdown of complex organic compounds into simpler ones Generally hydrolytic reactions (water used to break bonds) - PowerPoint PPT PresentationTRANSCRIPT
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Chapter 5: Microbial Metabolism
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METABOLISM• Sum of all chemical
rxn’s within a living organism
• Release energy• Require energy
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CATABOLIC REACTION (breakdown)
• Breakdown of complex organic compounds into simpler ones
• Generally hydrolytic reactions (water used to break bonds)
• Exergonic (produce more energy than consume)
• Provide building blocks for anabolic rxns and energy needed to drive anabolic rxns
ANABOLIC REACTIONS (build)
• Building of complex organic molecules from simpler ones
• Generally dehydration synthesis
• Endergonic (consume more energy than produce)
ATP <=> ADP + Pi + energy
Rxn’s all determined by enzymesand coupled together
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Metabolism: Overview
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But reactions happen very SLOWLY by
themselves....to make reactions go FASTER we
need.........
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ENZYMES !
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Enzymes• Biological catalysts• Each act on a specific substance (substrate)• Has active site for substrate to bind to
– “loading dock”• Each catalyzes only one reaction• Sensitive to temperature, pH, concentration• Can become denatured• All end in -ase
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Enzymes• Large 3-D globular molecule
• Composed of two parts1. Protein portion (apoenzyme)– Inactive if alone
2. Nonprotein component (cofactor)– Ions of iron, zinc, magnesium or
calcium– If organic = coenzyme
• Together with the apoenzyme and cofactor = active enzyme (holoenzyme)
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Enzymes: Steps in a Reaction
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Enzymes: Overview
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HOW DO YOU
CONTROL GROWTH OF BACTERIA??
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CONTROL THEIR
ENZYMES!(Using inhibitors)
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InhibitorsCompetitive Inhibitors• Fill the active site of an enzyme
and compete with the normal substrate for the active site
• Similar shape and chemical structure
• Does not produce products• Example: Sulfa drug
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InhibitorsNoncompetitive Inhibitors• Do not compete with the substrate; instead interact with
another part of the enzyme• Binds and causes the active site to change its shape, making
it nonfunctional• Allosteric inhibition “other space”Animation: CD
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Carbohydrate Catabolism• Most microorganisms use
carbon as primary energy source
• Oxidation of glucose1. Cellular Respiration
Glycolysis Krebs cycle Electron Transport Chain Lots of energy made
2. Fermentation Glycolysis Products: Alcohol or lactic acid Little energy made
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Fermentation• Releases energy
from sugars or other organic molecules
• Does not require oxygen
• Produces only a small amount of ATP
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Alcohol Fermentation: •Saccharomyces (yeast)
Lactic Acid Fermentation•Streptococcus• Lactobacillus• Bacillus
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Photosynthesis
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• Conversion of light energy into chemical energy• Chemical energy then used to convert CO2 into
sugars (carbon fixation)• Cyanobacteria
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REVIEW
• Cellular Respiration– Aerobic– Carbon
• Fermentation– Anaerobic– Carbon
• Photosynthesis– Carbon Fixation– Light energy
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Classifying Bacteria-Nutritional Pattern
HOW DO THEY GET
ENERGY?Phototrophs: use light as
primary energy sourceChemotrophs: use
reactions of inorganic or organic compounds for energy
HOW DO THEY GET CARBON?Autotrophs: CO2 as principle
carbon sourceHeterotrophs: organic
carbon source
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PHOTOAUTOTROPHSEnergy: lightCarbon: CO2
Examples: cyanobacteria(photosynthesis)
Green and Purple Bacteria - use sulfur or hydrogen gas to reduce CO2 and make organic compounds
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Chromatium -Purple BacteriaFound in sulfide-containing freshwaterhabitats
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Chlorobium- Green Bacteriafound in hotsprings, cold lakesand sediments
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PHOTOHETEROTROPHS
Energy: LightCarbon: organic compoundsExamples:Green nonsulfur bacteria
Chloroflexus (found in hot springs, lakes,
hyersaline environments)
Purple nonsulfur bacteriaRhodopseudomonas
(found in soil and marine environments)
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CHEMOAUTOTROPHS
Energy: inorganic compoundsEx: H2S, S, NH3, H2, CO
Carbon: CO2
-fix CO2
Example:Beggiatoa - use H2S, found in soil,
sulfur springs, mud layers of lakes,
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CHEMOHETEROTROPHS
Energy: organic compoundsEx: glucose
*use the electrons from H-atoms as energy source
Carbon: organic compound*Hard to distinguish-use the same
compound
Example:Streptococcus pneumonia - fermentation
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• http://people.eku.edu/ritchisong/RITCHISO/energyflowchart.jpg
• http://www.hepafilters.com/images/microbes.jpg• http://www.bio12.com/ch6/RemedialEnzymes_file
s/image007.jpg• http://classes.midlandstech.edu/carterp/Courses/bi
o225/chap05/Slide13.GIF