chapter 12 dna and rna - viggenz.com · chapter 12 dna and rna ... replicated, how do the new...
TRANSCRIPT
Prentice Hall Biology
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Chapter 12 DNA and RNA
These models show the structure of DNA the molecule that carries genetic information
How do codes work
Procedure
1 Obtain 12 pop beads of four different colors2 Select a word that contains at least five different letters from the text on the opening screen of 12ndash1 Use
your beads to develop a code for your word3 Exchange your code and your coded bead chain with a classmate Use the classmates code to decipher his
or her word
Think About It
1 Using Models How were you able to encode five different letters using only four colors2 Calculating How many different letters could you encode by using two beads to stand for each letter used
in your message3 Analyzing Data Could you encode the whole alphabet by using three beads for each letter
Prentice Hall Biology
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Chapter 12 DNA and RNA
Click the button above to open multiple-choice questions
Click the button above to open short-answer questions
Click the button above to open critical-thinking questions
Click the button above to open a performance-based assessment
For An interactive self -test
Prentice Hall Biology
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Chapter 12 DNA and RNA
Understanding Concepts
11 As scientists tried to discover the nature of genes what three criticalgene functions had they identified
12 Describe the components and structure of a DNA nucleotide13 Explain how Chargaffs rules helped Watson and Crick model DNA
14 What is meant by the term base pairing How is base pairing involvedin DNA replication
15 Describe the appearance of DNA in a typical prokaryotic cell16 Explain the process of replication When a DNA molecule is
replicated how do the new molecules relate to the original molecule
17 Describe the relationship between DNA chromatin histones andnucleosomes
18 What is the difference between exons and introns19 What is a codon20 What is an anticodon How does it function21 If a code on a DNA molecule for a specific amino acid is CTA what
would be the messenger RNA codon The transfer RNA anticodon
22 Explain why controlling the proteins in an organism controls theorganisms characteristics
23 Name two major types of mutations What do they have in commonHow are they different Give an example of each
24 Describe how a TATA box helps position RNA polymerase in aeukaryotic cell
25 Describe the role of an operon in a prokaryotic cell and give anexample of how an operon works
Prentice Hall Biology
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Chapter 12 DNA and RNA
Critical Thinking
26 Interpreting Graphics Look back at Griffiths experiment shown inthe figure Griffiths Experiment Describe the occasion in which thebacterial DNA withstood conditions that killed the bacteria Describewhat happened to the DNA from that point until the end of theexperiment
27 Using Models Evaluate Watson and Cricks model of the DNAmolecule How adequately does it represent the structure of DNA
28 Using Analogies Is photocopying a document similar to DNAreplication Think of the original materials the copying process andthe final products Explain how the two processes are alike Identifymajor differences
29 Applying Concepts Suppose you start with two DNA strandsACCGTCAC and TCGCACGT Use the ldquorulesrdquo of base pairing to listthe bases on messenger RNA strands transcribed from those DNAstrands
30 Predicting Examine the first intron in the diagram below Whatdifference would result in the protein produced by the messenger RNAif that intron were not removed but instead functioned as an exon
31 Using Analogies The word transcribe means ldquoto write outrdquo and theword translate means ldquoto express in another languagerdquo Review themeanings of transcription and translation in genetics How do thetechnical meanings of these words relate to meanings of the words inordinary language
32 Inferring Rosalind Franklins X-ray patterns showed that the distancebetween the two phosphate-sugar ldquobackbonesrdquo of a DNA molecule isthe same throughout the length of the molecule How did thatinformation help Watson and Crick determine how the bases arepaired
33 Comparing and Contrasting How does the possible impact of achromosomal mutation that occurs during meiosis differ from that of a
Prentice Hall Biology
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similar event that occurs during mitosis of a body cell not involved inreproduction
34 Predicting A researcher identifies the nucleotide sequence AAC in along strand of RNA inside a nucleus In the genetic code AAC codesfor the amino acid asparagine When that RNA becomes involved inprotein synthesis will asparagine necessarily appear in the proteinExplain
35 Connecting Concepts Recall what you learned about mitosis inChapter 10 and meiosis in Chapter 11 Describe what happens to acells DNA during each of these processes
Writing in ScienceRecall that Gregor Mendel concluded that factors which we now call genes determine the traits that are passed fromone generation to the next Imagine that you could send a letter backwards in time to Mendel Write a letter to him inwhich you explain what a gene consists of in molecular terms In your letter you will need to explain briefly whatDNA and proteins are (Hints Your explanation can be simple you do not have to include the details of transcriptionand translation Review Chapter 2 for the definition of protein)
Prentice Hall Biology
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Chapter 12 DNA and RNA
Performance-Based AssessmentMake a Model Make a three-dimensional model representing protein synthesis Your ldquoproteinrdquo should be a sequenceof three different amino acids Show the DNA molecule and the related RNA molecules that would be involved inproducing your protein
Prentice Hall Biology
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Key Concepts
What did scientistsdiscover about therelationship between genesand DNA
What is the overallstructure of the DNAmolecule
Vocabulary transformation bacteriophage nucleotide base pairingReading StrategySummarizing As you readfind the key ideas for the textunder each blue heading Writedown a few key words fromeach main idea Then use thekey words in your summaryRevise your summary keepingonly the most important ideas
12ndash1 DNA
How do genes work What are they made of and how do they determine thecharacteristics of organisms Are genes single molecules or are they longerstructures made up of many molecules In the middle of the 1900s questionslike these were on the minds of biologists everywhere
To truly understand genetics biologists first had to discover the chemicalnature of the gene If the structures that carry genetic information could beidentified it might be possible to understand how genes control the inheritedcharacteristics of living things
Prentice Hall Biology
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12ndash1 DNA (continued)Griffith and Transformation
Like many stories in science the discovery of the molecular nature of the gene beganwith an investigator who was actually looking for something else In 1928 Britishscientist Frederick Griffith was trying to figure out how bacteria make people sickMore specifically Griffith wanted to learn how certain types of bacteria produce aserious lung disease known as pneumonia
Griffith had isolated two slightly different strains or types of pneumonia bacteriafrom mice Both strains grew very well in culture plates in his lab but only one of thestrains caused pneumonia The disease-causing strain of bacteria grew into smoothcolonies on culture plates whereas the harmless strain produced colonies with roughedges The differences in appearance made the two strains easy to distinguish
Griffiths Experiments When Griffith injected mice with the disease-causing strain ofbacteria the mice developed pneumonia and died When mice were injected with theharmless strain they didnt get sick at all Griffith wondered if the disease-causingbacteria might produce a poison
To find out he took a culture of these cells heated the bacteria to kill them andinjected the heat-killed bacteria into mice The mice survived suggesting that thecause of pneumonia was not a chemical poison released by the disease-causingbacteria Griffiths experiments are shown in the figure at right
Griffiths Experiments
Transformation Griffiths next experiment produced an amazing result He mixed hisheat-killed disease-causing bacteria with live harmless ones and injected the mixtureinto mice By themselves neither should have made the mice sick But to Griffithsamazement the mice developed pneumonia and many died When he examined thelungs of the mice he found them filled not with the harmless bacteria but with thedisease-causing bacteria Somehow the heat-killed bacteria had passed their disease-causing ability to the harmless strain Griffith called this process transformationbecause one strain of bacteria (the harmless strain) had apparently been changedpermanently into another (the disease-causing strain)
Griffiths Experiments
Griffith hypothesized that when the live harmless bacteria and the heat-killed bacteriawere mixed some factor was transferred from the heat-killed cells into the live cellsThat factor he hypothesized must contain information that could change harmlessbacteria into disease-causing ones Furthermore since the ability to cause disease wasinherited by the transformed bacterias offspring the transforming factor might be agene
Prentice Hall Biology
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Prentice Hall Biology
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12ndash1 DNA (continued)Avery and DNA
In 1944 a group of scientists led by Canadian biologist Oswald Avery at theRockefeller Institute in New York decided to repeat Griffiths work They did so todetermine which molecule in the heat-killed bacteria was most important fortransformation If transformation required just one particular molecule that might wellbe the molecule of the gene
Avery and his colleagues made an extract or juice from the heat-killed bacteria Theythen carefully treated the extract with enzymes that destroyed proteins lipidscarbohydrates and other molecules including the nucleic acid RNA Transformationstill occurred Obviously since these molecules had been destroyed they were notresponsible for the transformation
Avery and the other scientists repeated the experiment this time using enzymes thatwould break down DNA When they destroyed the nucleic acid DNA in the extracttransformation did not occur There was just one possible conclusion DNA was thetransforming factor Avery and other scientists discovered that the nucleic acidDNA stores and transmits the genetic information from one generation of anorganism to the next
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12ndash1 DNA (continued)The Hershey-Chase Experiment
Scientists are a skeptical group It usually takes several experiments to convince themof something as important as the chemical nature of the gene The most important ofthese experiments was performed in 1952 by two American scientists Alfred Hersheyand Martha Chase They collaborated in studying viruses nonliving particles smallerthan a cell that can infect living organisms
Bacteriophages One kind of virus that infects bacteria is known as a bacteriophage(bak-TEER-ee-uh-fayj) which means ldquobacteria eaterrdquo Bacteriophages are composedof a DNA or RNA core and a protein coat When a bacteriophage enters a bacteriumthe virus attaches to the surface of the cell and injects its genetic information into itThe viral genes act to produce many new bacteriophages and they gradually destroythe bacterium When the cell splits open hundreds of new viruses burst out
Radioactive Markers Hershey and Chase reasoned that if they could determine whichpart of the virusmdashthe protein coat or the DNA coremdashentered the infected cell theywould learn whether genes were made of protein or DNA To do this they grewviruses in cultures containing radioactive isotopes of phosphorus-32 (32P) and sulfur-35 (35S) This was a clever strategy because proteins contain almost no phosphorusand DNA contains no sulfur The radioactive substances could be used as markers If35S was found in the bacteria it would mean that the viruses protein had been injectedinto the bacteria If 32P was found in the bacteria then it was the DNA that had beeninjected Click the article at right for more information on radioisotopes
Radioisotopes
The Hershey-Chase experiment is shown in the figure at right The two scientistsmixed the marked viruses with bacteria Then they waited a few minutes for theviruses to inject their genetic material Next they separated the viruses from thebacteria and tested the bacteria for radioactivity Nearly all the radioactivity in thebacteria was from phosphorus (32P) the marker found in DNA Hershey andChase concluded that the genetic material of the bacteriophage was DNA notprotein
The Hershey-ChaseExperiment
Prentice Hall Biology
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Prentice Hall Biology
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12ndash1 DNA (continued)The Components and Structure of DNA
You might think that knowing genes were made of DNA would have satisfiedscientists but that was not the case at all Instead they wondered how DNA or anymolecule for that matter could do the three critical things that genes were known todo First genes had to carry information from one generation to the next second theyhad to put that information to work by determining the heritable characteristics oforganisms and third genes had to be easily copied because all of a cells geneticinformation is replicated every time a cell divides For DNA to do all of that it wouldhave to be a very special molecule indeed
For Links on DNAVisit wwwSciLinksorgWeb Code cbn-4121
DNA is a long molecule made up of units called nucleotides As the figure belowshows each nucleotide is made up of three basic components a 5-carbon sugar calleddeoxyribose a phosphate group and a nitrogenous (nitrogen-containing) base Thereare four kinds of nitrogenous bases in DNA Two of the nitrogenous bases adenine(AD-uh-neen) and guanine (GWAH-neen) belong to a group of compounds known aspurines The remaining two bases cytosine (SY-tuh-zeen) and thymine (THY-meen)are known as pyrimidines Purines have two rings in their structures whereaspyrimidines have one ring
DNA Nucleotides DNA is made up of nucleotides Each nucleotide has three parts a deoxyribose molecule aphosphate group and a nitrogenous base There are four different bases in DNA adenine guanine cytosine and thymine
The backbone of a DNA chain is formed by sugar and phosphate groups of eachnucleotide The nitrogenous bases stick out sideways from the chain The nucleotidescan be joined together in any order meaning that any sequence of bases is possible
If you dont see much in the figure above that could explain the remarkable propertiesof the gene dont be surprised In the 1940s and early 1950s the leading biologists in
Prentice Hall Biology
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the world thought of DNA as little more than a string of nucleotides They werebaffled too The four different nucleotides like the 26 letters of the alphabet could bestrung together in many different ways so it was possible they could carry codedgenetic information However so could many other molecules at least in principleWas there something more to the structure of DNA
Chargaffs Rules One of the puzzling facts about DNA was a curious relationshipbetween its nucleotides Years earlier Erwin Chargaff an American biochemist haddiscovered that the percentages of guanine [G] and cytosine [C] bases are almost equalin any sample of DNA The same thing is true for the other two nucleotides adenine[A] and thymine [T] as shown in the table below The observation that [A] = [T] and[G] = [C] became known as Chargaffs rules Despite the fact that DNA samples fromorganisms as different as bacteria and humans obeyed this rule neither Chargaff noranyone else had the faintest idea why
Chargaffs Rules Erwin Chargaff showed that the percentages of guanine and cytosine in DNA are almost equalThe same is true for adenine and thymine
X-Ray Evidence In the early 1950s a British scientist named Rosalind Franklinbegan to study DNA She used a technique called X-ray diffraction to get informationabout the structure of the DNA molecule Aiming a powerful X-ray beam atconcentrated DNA samples she recorded the scattering pattern of the X-rays on filmFranklin worked hard to make better and better patterns from DNA until the patternsbecame clear
By itself Franklins X-ray pattern does not reveal the structure of DNA but it doescarry some very important clues The X-shaped pattern in the photograph in the imagebelow shows that the strands in DNA are twisted around each other like the coils of aspring a shape known as a helix The angle of the X suggests that there are twostrands in the structure Other clues suggest that the nitrogenous bases are near thecenter of the molecule
Prentice Hall Biology
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X-Ray Diffraction Image of DNA X-ray diffraction is the method that Rosalind Franklin used to studyDNA
The Double Helix At the same time that Franklin was continuing her researchFrancis Crick a British physicist and James Watson an American biologist weretrying to understand the structure of DNA by building three-dimensional models ofthe molecule Their models were made of cardboard and wire They twisted andstretched the models in various ways but their best efforts did nothing to explainDNAs properties
Then early in 1953 Watson was shown a copy of Franklins remarkable X-raypattern The effect was immediate In his book The Double Helix Watson wrote ldquoTheinstant I saw the picture my mouth fell open and my pulse began to racerdquo Using cluesfrom Franklins pattern within weeks Watson and Crick had built a structural modelthat explained the puzzle of how DNA could carry information and how it could becopied They published their results in a historic one-page paper in April of 1953 Watson and Cricks model of DNA was a double helix in which two strands werewound around each other
Discovering the Role of DNA
A double helix looks like a twisted ladder or a spiral staircase When Watson andCrick evaluated their DNA model they realized that the double helix accounted formany of the features in Franklins X-ray pattern but did not explain what forces heldthe two strands together They then discovered that hydrogen bonds could formbetween certain nitrogenous bases and provide just enough force to hold the twostrands together As the figure below shows hydrogen bonds can form only betweencertain base pairsmdashadenine and thymine and guanine and cytosine Once they sawthis they realized that this principle called base pairing explained Chargaffs rulesNow there was a reason that [A] = [T] and [G] = [C] For every adenine in a double-stranded DNA molecule there had to be exactly one thymine molecule for eachcytosine molecule there was one guanine molecule
Prentice Hall Biology
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DNA Structure DNA is a double helix in which two strands are wound around each other Each strand ismade up of a chain of nucleotides The two strands are held together by hydrogen bonds between adenine and thymine andbetween guanine and cytosine
Prentice Hall Biology
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12ndash1 DNA
1 Key Concept List the conclusions Griffith Avery Hershey and Chase drew from their experiments2 Key Concept Describe Watson and Cricks model of the DNA molecule 3 What are the four kinds of bases found in DNA 4 Did Watson and Cricks model account for the equal amounts of thymine and adenine in DNA Explain
5 Critical Thinking Inferring Why did Hershey and Chase grow viruses in cultures that contained bothradioactive phosphorus and radioactive sulfur What might have happened if they had used only oneradioactive substance
Scientific MethodsUsing the experiments of Griffith Avery or Hershey and Chase as an example develop a flowchart that showshow the scientist or scientists used scientific processes Be sure to identify each process Hint You may wish toreview Chapter 1 which describes scientific methods
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Key Concept
What happens duringDNA replication
Vocabulary chromatin histone replication DNA polymeraseReading Strategy AskingQuestions Before you readstudy the figure DNAReplication Make a list ofquestions about the diagramAs you read write down theanswers to your questions
12ndash2 Chromosomes and DNA Replication
DNA is present in such large amounts in many tissues that its easy to extractand analyze But where is DNA found in the cell How is it organized Whereare the genes that Mendel first described a century and a half ago
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12ndash2 Chromosomes and DNA Replication (continued)DNA and Chromosomes
Prokaryotic cells lack nuclei and many of the organelles found in eukaryotes TheirDNA molecules are located in the cytoplasm Most prokaryotes have a single circularDNA molecule that contains nearly all of the cells genetic information This largeDNA molecule is usually referred to as the cells chromosome as shown in the figureat right
Prokaryote Chromosome
Eukaryotic DNA is a bit more complicated Many eukaryotes have as much as 1000times the amount of DNA as prokaryotes This DNA is not found free in thecytoplasm Eukaryotic DNA is generally located in the cell nucleus in the form of anumber of chromosomes The number of chromosomes varies widely from one speciesto the next For example diploid human cells have 46 chromosomes Drosophila cellshave 8 and giant sequoia tree cells have 22
DNA Length DNA molecules are surprisingly long The chromosome of theprokaryote E coli which can live in the human colon (large intestine) contains4639221 base pairs The length of such a DNA molecule is roughly 16 mm whichdoesnt sound like much until you think about the small size of a bacterium To fitinside a typical bacterium the DNA molecule must be folded into a space only oneone-thousandth of its length
To get a rough idea of what this means think of a large school backpack Thenimagine trying to pack a 300-meter length of rope into the backpack The DNA mustbe dramatically folded to fit within the cell
Chromosome Structure The DNA in eukaryotic cells is packed even more tightly Ahuman cell contains almost 1000 times as many base pairs of DNA as a bacteriumThe nucleus of a human cell contains more than 1 meter of DNA How is so muchDNA folded into tiny chromosomes The answer can be found in the composition ofeukaryotic chromosomes
Eukaryotic chromosomes contain both DNA and protein tightly packed together toform a substance called chromatin Chromatin consists of DNA that is tightly coiledaround proteins called histones as shown in the activity at right Together the DNAand histone molecules form a beadlike structure called a nucleosome Nucleosomespack with one another to form a thick fiber which is shortened by a system of loopsand coils
Eukaryotic ChromosomeStructure
During most of the cell cycle these fibers are dispersed in the nucleus so thatindividual chromosomes are not visible During mitosis however the fibers of each
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individual chromosome are drawn together forming the tightly packed chromosomesyou can see through a light microscope in dividing cells The tight packing ofnucleosomes may help separate chromosomes during mitosis There is also someevidence that changes in chromatin structure and histone-DNA binding are associatedwith changes in gene activity and expression
What do nucleosomes do Nucleosomes seem to be able to fold enormous lengths ofDNA into the tiny space available in the cell nucleus This is such an importantfunction that the histone proteins themselves have changed very little during evolutionmdashprobably because mistakes in DNA folding could harm a cells ability to reproduce
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12ndash2 Chromosomes and DNA Replication (continued)DNA Replication
When Watson and Crick discovered the double helix structure of DNA there was onemore remarkable aspect that they recognized immediately The structure explainedhow DNA could be copied or replicated Each strand of the DNA double helix has allthe information needed to reconstruct the other half by the mechanism of base pairingBecause each strand can be used to make the other strand the strands are said to becomplementary If you could separate the two strands the rules of base pairing wouldallow you to reconstruct the base sequence of the other strand
For Links on DNA replicationVisit wwwSciLinksorgWeb Code cbn-4122
In most prokaryotes DNA replication begins at a single point in the chromosome andproceeds often in two directions until the entire chromosome is replicated In thelarger eukaryotic chromosomes DNA replication occurs at hundreds of placesReplication proceeds in both directions until each chromosome is completely copiedThe sites where separation and replication occur are called replication forks
Duplicating DNA Before a cell divides it duplicates its DNA in a copying processcalled replication This process ensures that each resulting cell will have a completeset of DNA molecules During DNA replication the DNA molecule separatesinto two strands then produces two new complementary strands following therules of base pairing Each strand of the double helix of DNA serves as atemplate or model for the new strand The figure at right shows how this processoccurs
DNA Replication
The figure DNA Replication shows the process of DNA replication The two strandsof the double helix have separated allowing two replication forks to form As eachnew strand forms new bases are added following the rules of base pairing In otherwords if the base on the old strand is adenine thymine is added to the newly formingstrand Likewise guanine is always paired to cytosine
For DNA Replication activityVisit PHSchoolcomWeb Code cbp-4122
For example a strand that has the bases TACGTT produces a strand with thecomplementary bases ATGCAA The result is two DNA molecules identical to eachother and to the original molecule Note that each DNA molecule resulting fromreplication has one original strand and one new strand
How Replication Occurs DNA replication is carried out by a series of enzymesThese enzymes ldquounziprdquo a molecule of DNA The unzipping occurs when the hydrogenbonds between the base pairs are broken and the two strands of the molecule unwindEach strand serves as a template for the attachment of complementary bases
DNA Replication
DNA replication involves a host of enzymes and regulatory molecules You may recall
Prentice Hall Biology
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that enzymes are highly specific For this reason they are often named for thereactions they catalyze The principal enzyme involved in DNA replication is calledDNA polymerase (PAHL-ih-mur-ayz) because it joins individual nucleotides toproduce a DNA molecule which is of course a polymer DNA polymerase alsoldquoproofreadsrdquo each new DNA strand helping to maximize the odds that each moleculeis a perfect copy of the original DNA
Synthesis of New DNA MoleculesHow can you investigate when and where cells synthesize DNAScientists have done this by briefly adding radioactively labeledthymine to the medium in which a cell grows A cell that issynthesizing DNA will take the labeled thymine nucleotide into itsDNA The graph shows the total amount of radioactive label takeninto DNA from thymine during an eight-hour period between twocell divisions
1 Interpreting Graphics Contrast the amounts of radioactivityincorporated during the following times (a) the first fourhours of the experiment (b) the next two hours and (c) thefinal two hours
2 Drawing Conclusions Is DNA synthesized continuallyduring the cell cycle (the period between cell divisions) Ifnot during what phase is it synthesized How long is that phase
3 Predicting Which organelle or cell structure would you expect to contain the most radioactivity after thisexperiment Explain
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12ndash2 Chromosomes and DNA Replication
1 Key Concept Explain how DNA is replicated 2 Where and in what form is eukaryotic DNA found 3 How are the long DNA molecules found in eukaryotes packed into short chromosomes 4 How are histones related to nucleosomes 5 What is the role of DNA polymerase in DNA replication 6 Critical Thinking Comparing and Contrasting How is the structure of chromosomes in eukaryotes different
from the structure of chromosomes in prokaryotes
Creating a Venn DiagramMake a Venn diagram that compares the process of DNA replication in prokaryotes and eukaryotes Comparethe location steps and end products of the process in each kind of cell
Prentice Hall Biology
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Key Concepts
What are the three maintypes of RNA
What is transcription
What is translation
Vocabulary gene messenger RNA (mRNA) ribosomal RNA (rRNA) transfer RNA (tRNA) transcription RNA polymerase promoter intron exon codon translation anticodonReading Strategy UsingVisuals Before you readpreview the figureTranslation As you readnotice what happens in eachstep of translation or proteinsynthesis
12ndash3 RNA and Protein Synthesis
The double helix structure explains how DNA can be copied but it does notexplain how a gene works In molecular terms genes are coded DNAinstructions that control the production of proteins within the cell The firststep in decoding these genetic messages is to copy part of the nucleotidesequence from DNA into RNA or ribonucleic acid These RNA moleculescontain coded information for making proteins
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12ndash3 RNA and Protein Synthesis (continued)The Structure of RNA
RNA like DNA consists of a long chain of nucleotides As you may recall eachnucleotide is made up of a 5-carbon sugar a phosphate group and a nitrogenous baseThere are three main differences between RNA and DNA The sugar in RNA is riboseinstead of deoxyribose RNA is generally single-stranded and RNA contains uracil inplace of thymine
You can think of an RNA molecule as a disposable copy of a segment of DNA Inmany cases an RNA molecule is a working copy of a single gene The ability to copya single DNA sequence into RNA makes it possible for a single gene to producehundreds or even thousands of RNA molecules
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12ndash3 RNA and Protein Synthesis (continued)Types of RNA
RNA molecules have many functions but in the majority of cells most RNAmolecules are involved in just one jobmdashprotein synthesis The assembly of aminoacids into proteins is controlled by RNA There are three main types of RNAmessenger RNA ribosomal RNA and transfer RNA The structures of thesemolecules are shown in the figure at right
Types of RNA
Most genes contain instructions for assembling amino acids into proteins The RNAmolecules that carry copies of these instructions are known as messenger RNA(mRNA) because they serve as ldquomessengersrdquo from DNA to the rest of the cell
Proteins are assembled on ribosomes shown in the model below Ribosomes are madeup of several dozen proteins as well as a form of RNA known as ribosomal RNA(rRNA)
During the construction of a protein a third type of RNA molecule transfers eachamino acid to the ribosome as it is specified by coded messages in mRNA TheseRNA molecules are known as transfer RNA (tRNA)
Model of Ribosome In this detailed model of a ribosome the two subunits of the ribosome are shown in yellowand blue The model was produced using cryo-electron microscopy Data from more than 73000 electron micrographstaken at ultra-cold temperatures to preserve ribosome structure were analyzed to produce the model
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12ndash3 RNA and Protein Synthesis (continued)Transcription
RNA molecules are produced by copying part of the nucleotide sequence of DNA intoa complementary sequence in RNA a process called transcription Transcriptionrequires an enzyme known as RNA polymerase that is similar to DNA polymerase
During transcription RNA polymerase binds to DNA and separates the DNAstrands RNA polymerase then uses one strand of DNA as a template from whichnucleotides are assembled into a strand of RNA The process of transcription isshown in the figure at right
Transcription
How does RNA polymerase ldquoknowrdquo where to start and stop making an RNA copy ofDNA The answer to this question begins with the observation that RNA polymerasedoesnt bind to DNA just anywhere The enzyme will bind only to regions of DNAknown as promoters which have specific base sequences In effect promoters aresignals in DNA that indicate to the enzyme where to bind to make RNA Similarsignals in DNA cause transcription to stop when the new RNA molecule is completed
DNA Transcription
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12ndash3 RNA and Protein Synthesis (continued)RNA Editing
Like a writers first draft many RNA molecules require a bit of editing before they areready to go into action Remember that an RNA molecule is produced by copyingDNA Surprisingly the DNA of eukaryotic genes contains sequences of nucleotidescalled introns that are not involved in coding for proteins The DNA sequences thatcode for proteins are called exons because they are ldquoexpressedrdquo in the synthesis ofproteins When RNA molecules are formed both the introns and the exons are copiedfrom the DNA However the introns are cut out of RNA molecules while they are stillin the nucleus The remaining exons are then spliced back together to form the finalmRNA as shown in the figure below
RNA Editing Many RNA molecules have sections called introns edited out of them before they become functionalThe remaining pieces called exons are spliced together Then a cap and tail are added to form the final RNA molecule
Why do cells use energy to make a large RNA molecule and then throw parts of itaway Thats a good question and biologists still do not have a complete answer to itSome RNA molecules may be cut and spliced in different ways in different tissuesmaking it possible for a single gene to produce several different forms of RNAIntrons and exons may also play a role in evolution This would make it possible forvery small changes in DNA sequences to have dramatic effects in gene expression
Prentice Hall Biology
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12ndash3 RNA and Protein Synthesis (continued)The Genetic Code
Proteins are made by joining amino acids into long chains called polypeptides Eachpolypeptide contains a combination of any or all of the 20 different amino acids Theproperties of proteins are determined by the order in which different amino acids arejoined together to produce polypeptides How you might wonder can a particularorder of nitrogenous bases in DNA and RNA molecules be translated into a particularorder of amino acids in a polypeptide
The ldquolanguagerdquo of mRNA instructions is called the genetic code As you know RNAcontains four different bases A U C and G In effect the code is written in alanguage that has only four ldquolettersrdquo How can a code with just four letters carryinstructions for 20 different amino acids The genetic code is read three letters at atime so that each ldquowordrdquo of the coded message is three bases long Each three-letterldquowordrdquo in mRNA is known as a codon as shown in the figure below A codonconsists of three consecutive nucleotides that specify a single amino acid that is to beadded to the polypeptide For example consider the following RNA sequence
This sequence would be read three bases at a time as
The codons represent the different amino acids
Codon A codon is a group of three nucleotides on messenger RNA that specify a particular amino acid
Prentice Hall Biology
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Because there are four different bases there are 64 possible three-base codons (4 times 4times 4 = 64) The figure at right shows all 64 possible codons of the genetic code As youcan see some amino acids can be specified by more than one codon For example sixdifferent codons specify the amino acid leucine and six others specify arginine
Genetic Code
There is also one codon AUG that can either specify methionine or serve as theinitiation or ldquostartrdquo codon for protein synthesis Notice also that there are three ldquostoprdquocodons that do not code for any amino acid Stop codons act like the period at the endof a sentence they signify the end of a polypeptide which consists of many aminoacids
How does a cell interpret DNA
Procedure 1 A certain gene has the following sequence of nucleotides
GACAAGTCCACAATCWrite this sequence on a sheet of paper
2 From left to right write the sequence of the mRNA molecule transcribed from this gene3 Look at the figure Genetic Code Reading the mRNA codons from left to right write the amino acid
sequence of the polypeptide translated from the mRNA4 Repeat step 3 reading the codons from right to left
Analyze and Conclude 1 Applying Concepts Why did steps 3 and 4 produce different polypeptides2 Inferring Do cells usually decode nucleotides in one direction only or in either direction
Prentice Hall Biology
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12ndash3 RNA and Protein Synthesis (continued)Translation
The sequence of nucleotide bases in an mRNA molecule serves as instructions for theorder in which amino acids should be joined together to produce a polypeptideHowever anyone who has tried to assemble a complex toy knows that instructionsgenerally dont do the job themselves They need something to read them and put themto use In the cell that ldquosomethingrdquo is a tiny factory called the ribosome
The decoding of an mRNA message into a polypeptide chain (protein) is known astranslation Translation takes place on ribosomes During translation the celluses information from messenger RNA to produce proteins Refer to the figure atright as you read about translation
Translation
Before translation occurs messenger RNA is transcribed from DNA in thenucleus and released into the cytoplasm
For Protein synthesisactivityVisit PHSchoolcomWeb Code cbp-4123
Translation begins when an mRNA molecule in the cytoplasm attaches to aribosome As each codon of the mRNA molecule moves through the ribosome theproper amino acid is brought into the ribosome by tRNA In the ribosome the aminoacid is transferred to the growing polypeptide chain
Protein Synthesis
Each tRNA molecule carries only one kind of amino acid For example some tRNAmolecules carry methionine others carry arginine and still others carry serine Inaddition to an amino acid each tRNA molecule has three unpaired bases These basescalled the anticodon are complementary to one mRNA codon
In the case of the tRNA molecule for methionine the anticodon bases are UAC whichpair with the methionine codon AUG The ribosome has a second binding site for atRNA molecule for the next codon If that next codon is UUC a tRNA molecule withan AAG anticodon would fit against the mRNA molecule held in the ribosome Thatsecond tRNA molecule would bring the amino acid phenylalanine into the ribosome
Like an assembly line worker who attaches one part to another the ribosomeforms a peptide bond between the first and second amino acids methionine and
Prentice Hall Biology
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phenylalanine At the same time the ribosome breaks the bond that had held the firsttRNA molecule to its amino acid and releases the tRNA molecule The ribosome thenmoves to the third codon where a tRNA molecule brings it the amino acid specifiedby the third codon
The polypeptide chain continues to grow until the ribosome reaches a stop codonon the mRNA molecule When the ribosome reaches a stop codon it releases thenewly formed polypeptide and the mRNA molecule completing the process oftranslation
Prentice Hall Biology
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12ndash3 RNA and Protein Synthesis (continued)The Roles of RNA and DNA
You can compare the different roles played by DNA and RNA molecules in directingprotein synthesis to the two types of plans used by builders A master plan has all theinformation needed to construct a building But builders never bring the valuablemaster plan to the building site where it might be damaged or lost Instead theyprepare inexpensive disposable copies of the master plan called blueprints The masterplan is safely stored in an office and the blueprints are taken to the job site Similarlythe cell uses the vital DNA ldquomaster planrdquo to prepare RNA ldquoblueprintsrdquo The DNAmolecule remains within the safety of the nucleus while RNA molecules go to theprotein-building sites in the cytoplasmmdashthe ribosomes
For Links on protein synthesisVisit wwwSciLinksorgWeb Code cbn-4123
Prentice Hall Biology
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12ndash3 RNA and Protein Synthesis (continued)Genes and Proteins
Gregor Mendel might have been surprised to learn that most genes contain nothingmore than instructions for assembling proteins as shown in the figure at right Hemight have asked what proteins could possibly have to do with the color of a flowerthe shape of a leaf a human blood type or the sex of a newborn baby
Assembling Proteins
The answer is that proteins have everything to do with these things Remember thatmany proteins are enzymes which catalyze and regulate chemical reactions A genethat codes for an enzyme to produce pigment can control the color of a flowerAnother gene produces an enzyme specialized for the production of red blood cellsurface antigen This molecule determines your blood type Genes for certain proteinscan regulate the rate and pattern of growth throughout an organism controlling its sizeand shape In short proteins are microscopic tools each specifically designed to buildor operate a component of a living cell
Prentice Hall Biology
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12ndash3 RNA and Protein Synthesis
1 Key Concept List the three main types of RNA 2 Key Concept What happens during transcription 3 Key Concept What happens during translation 4 Describe the three main differences between RNA and DNA 5 Critical Thinking Applying Concepts Using the genetic code identify the amino acids that have the
following messenger RNA strand codesUGGCAGUGC
Creative WritingAn RNA molecule is looking for a job in a protein synthesis factory and it asks you to write its reacutesumeacute ThisRNA molecule is not yet specialized and could with some structural changes function as either mRNA tRNAor rRNA The reacutesumeacute you create should reflect the qualifications needed for each type of RNA
Prentice Hall Biology
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Key Concept What are mutations
Vocabulary mutation point mutation frameshift mutation polyploidyReading Strategy UsingVisuals Before you readpreview the figures GeneMutations and ChromosomalMutations As you read noticethe changes that occur in geneand chromosomal mutations
12ndash4 Mutations
Now and then cells make mistakes in copying their own DNA inserting anincorrect base or even skipping a base as the new strand is put togetherThese mistakes are called mutations from a Latin word meaning ldquotochangerdquo Mutations are changes in the genetic material
Prentice Hall Biology
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12ndash4 Mutations (continued)Kinds of Mutations
Like the mistakes that people make in their daily lives mutations come in manyshapes and sizes Mutations that produce changes in a single gene are known as genemutations Those that produce changes in whole chromosomes are known aschromosomal mutations
Gene Mutations Gene mutations involving changes in one or a few nucleotides areknown as point mutations because they occur at a single point in the DNA sequencePoint mutations include substitutions in which one base is changed to another as wellas insertions and deletions in which a base is inserted or removed from the DNAsequence
Point Mutations
Substitutions usually affect no more than a single amino acid The effects of insertionsor deletions can be much more dramatic Remember that the genetic code is read inthree-base codons If a nucleotide is added or deleted the bases are still read in groupsof three but now those groupings are shifted for every codon that follows as shown inthe figure at right Changes like these are called frameshift mutations because theyshift the ldquoreading framerdquo of the genetic message By shifting the reading frameframeshift mutations may change every amino acid that follows the point of themutation Frameshift mutations can alter a protein so much that it is unable to performits normal functions
Gene Mutations
Chromosomal Mutations Chromosomal mutations involve changes in the number orstructure of chromosomes Such mutations may change the locations of genes onchromosomes and may even change the number of copies of some genes
Duplication and Deletion
Translocation and Inversion
The figure at right shows four types of chromosomal mutations deletionsduplications inversions and translocations Deletions involve the loss of all or part ofa chromosome while duplications produce extra copies of parts of a chromosomeInversions reverse the direction of parts of chromosomes and translocations occurwhen part of one chromosome breaks off and attaches to another
Chromosomal Mutations
Prentice Hall Biology
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Prentice Hall Biology
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12ndash4 Mutations (continued)Significance of Mutations
Many if not most mutations are neutral meaning that they have little or no effect onthe expression of genes or the function of the proteins for which they code Mutationsthat cause dramatic changes in protein structure or gene activity are often harmfulproducing defective proteins that disrupt normal biological activities Howevermutations are also the source of genetic variability in a species Some of this variationmay be highly beneficial
Harmful mutations are the causes of many genetic disorders you will learn aboutsome human genetic disorders in Chapter 14 Harmful mutations are also associatedwith many types of cancer In contrast beneficial mutations may produce proteinswith new or altered activities that can be useful to organisms in different or changingenvironments
Plant and animal breeders often take advantage of such beneficial mutations Forexample when a complete set of chromosomes fails to separate during meiosis thegametes that result may produce triploid (3N) or tetraploid (4N) organisms Thecondition in which an organism has extra sets of chromosomes is called polyploidyPolyploid plants are often larger and stronger than diploid plants Important cropplants have been produced in this way including bananas and many citrus fruits
Prentice Hall Biology
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12ndash4 Mutations
1 Key Concept What is a mutation 2 What is the significance of mutations to living things 3 What are two kinds of frameshift mutations 4 What are four types of chromosomal mutations 5 Critical Thinking Inferring The effects of a mutation are not always visible How might a biologist determine
whether a mutation has occurred and if so what type of mutation it is
CompareContrast ParagraphWrite a paragraph comparing and contrasting gene mutations and chromosomal mutations Hint To organizeyour ideas use a comparecontrast table The column heads might be Definition Types and Effects
Prentice Hall Biology
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Key Concepts
How are lac genes turnedoff and on
How are most eukaryoticgenes controlled
Vocabulary operon operator differentiation hox genesReading Strategy OutliningBefore you read use theheadings of the section to makean outline about generegulation As you read fill insubtopics and smaller topicsThen add phrases or asentence after each subtopic toprovide key information
12ndash5 Gene Regulation
Only a fraction of the genes in a cell are expressed at any given time Anexpressed gene is a gene that is transcribed into RNA How does the celldetermine which genes will be expressed and which will remain ldquosilentrdquo Aclose look at the structure of a gene provides some important clues
At first glance the DNA sequence of a gene is nothing more than a confusingjumble of the four letters that represent the bases in DNA However if wetake the time to analyze those letters patterns emerge Molecular biologistshave found that certain DNA sequences serve as promoters binding sites forRNA polymerase Others serve as start and stop signals for transcription Infact cells are filled with DNA-binding proteins that attach to specific DNAsequences and help to regulate gene expression A typical gene might looksomething like the figure below
A Typical Gene A typical gene includes start and stop signals with the nucleotides to be translated inbetween The DNA sequence shown is only a very small part of an actual gene
As weve seen there is a promoter just to one side of the gene But what arethe ldquoregulatory sitesrdquo next to the promoter These are places where otherproteins binding directly to the DNA sequences at those sites can regulatetranscription The actions of these proteins help to determine whether a geneis turned on or turned off
Prentice Hall Biology
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12ndash5 Gene Regulation (continued)Gene Regulation An Example
How does an organism ldquoknowrdquo whether to turn a gene on or off The commonbacterium E coli provides us with a perfect example of how gene expression can beregulated The 4288 protein-encoding genes in this bacterium include a cluster of threegenes that are turned on or off together A group of genes that operate together isknown as an operon Because these genes must be expressed in order for thebacterium to be able to use the sugar lactose as a food they are called the lac operon
For Articles on genetics
Why must E coli turn on the lac genes in order to use lactose for food Lactose is acompound made up of two simple sugars galactose and glucose To use lactose forfood the bacterium must take lactose across its cell membrane and then break thebond between glucose and galactose These tasks are performed by proteins coded forby the genes of the lac operon This means of course that if the bacterium is grown ina medium where lactose is the only food source it must transcribe the genes andproduce these proteins If grown on another food source such as glucose it wouldhave no need for these proteins
Remarkably the bacterium almost seems to ldquoknowrdquo when the products of these genesare needed The lac genes are turned off by repressors and turned on by thepresence of lactose This process tells us a great deal about how genes are regulated
On one side of the operons three genes are two regulatory regions In the promoter(P) RNA polymerase binds and then begins transcription The other region is theoperator (O) E coli cells contain several copies of a DNA-binding protein known asthe lac repressor which can bind to the O region As the figure below shows whenthe lac repressor binds to the O region RNA polymerase is prevented from beginningthe process of transcription In effect the binding of the repressor protein turns theoperon ldquooffrdquo by preventing the transcription of its genes
Prentice Hall Biology
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Function of the Lac Repressor The lac genes in E coli are turned off by repressors and turned on bythe presence of lactose When lactose is not present the repressor binds to the operator region preventing RNApolymerase from beginning transcription Lactose causes the repressor to be released from the operator region
If the repressor protein is always present how are the lac genes turned on in thepresence of lactose Besides its DNA binding site the lac repressor protein has abinding site for lactose itself When lactose is added to the medium in which E coli isgrowing sugar molecules diffuse into the cell and bind to the repressor proteins Thiscauses the repressor protein to change shape in a way that causes the repressor to falloff the operator Now with the repressor no longer bound to the O site RNApolymerase can bind to the promoter and transcribe the genes of the operon
The lac operon shows one way in which prokaryotic genes are regulated Many genesare regulated by repressor proteins while others use proteins that speed transcriptionSometimes regulation occurs at the level of protein synthesis Regardless of thesystem the result is the same Cells turn their genes on and off as needed
Prentice Hall Biology
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12ndash5 Gene Regulation (continued)Eukaryotic Gene Regulation
The general principles of gene regulation in prokaryotes also apply to eukaryotic cellsalthough there are some important differences Operons are generally not found ineukaryotes Most eukaryotic genes are controlled individually and haveregulatory sequences that are much more complex than those of the lac operon
The figure below shows some of the features of a typical eukaryotic gene One of themost interesting is a short region of DNA about 30 base pairs long containing asequence of TATATA or TATAAA before the start of transcription This region isfound before so many eukaryotic genes that it even has a name the ldquoTATA boxrdquo TheTATA box seems to help position RNA polymerase by marking a point just before thepoint at which transcription begins Eukaryotic promoters are usually found just beforethe TATA box and they consist of a series of short DNA sequences
Eukaryotic Gene Many eukaryotic genes include a sequence called the TATA box that may help position RNA
polymerase Eukaryotic genes have regulatory sequences that are more complex than prokaryotic genes
Genes are regulated in a variety of ways by enhancer sequences located before thepoint at which transcription begins An enormous number of proteins can bind todifferent enhancer sequences which is why eukaryotic gene regulation is so complexSome of these DNA-binding proteins enhance transcription by opening up tightlypacked chromatin Others help to attract RNA polymerase Still other proteins blockaccess to genes much like prokaryotic repressor proteins
Why is gene regulation in eukaryotes more complex than in prokaryotes Think for amoment about the way in which genes are expressed in a multicellular organism Thegenes that code for liver enzymes for example are not expressed in nerve cellsKeratin an important protein in skin cells is not produced in blood cells Cellspecialization requires genetic specialization but all of the cells in a multicellularorganism carry the complete genetic code in their nucleus Therefore for properoverall function only a tiny fraction of the available genes needs to be expressed inthe appropriate cells of different tissues throughout the body The complexity of generegulation in eukaryotes makes this specificity possible
Prentice Hall Biology
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12ndash5 Gene Regulation (continued)Development and Differentiation
Regulation of gene expression is especially important in shaping the way a complexorganism develops Each of the specialized cell types found in the adult develops fromthe same fertilized egg cell This means that cells dont just grow and divide duringembryonic development they also undergo differentiation meaning they becomespecialized in structure and function The study of genes that control development anddifferentiation is one of the most exciting areas in biology today
A series of genes known as the hox genes control the differentiation of cells andtissues in the embryo A mutation in one of these ldquomaster control genesrdquo cancompletely change the organs that develop in specific parts of the body Mutationsaffecting the hox genes in the fruit fly Drosophila for example can replace the flysantennae with legs growing on its head
In flies the hox genes are located side-by-side in a single cluster as shown in thefigure below Remarkably similar clusters exist in the DNA of other animalsincluding humans The function of the hox genes in humans seems to be almost thesamemdashto tell the cells of the body how they should differentiate as the body growsCareful control of expression in these genes is essential for normal development
Hox Genes In fruit flies a series of hox genes along a chromosome determines the basic structure of the flys bodyMice have very similar genes on four different chromosomes The color bars along the mouses back show the approximatebody area affected by genes of the corresponding colors
Prentice Hall Biology
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The striking similarity of genes that control development has a simple scientificexplanation Common patterns of genetic control exist because all these genes havedescended from the genes of common ancestors One such gene called Pax 6 controlseye growth in Drosophila A similar gene was found to guide eye growth in mice andother mammals When a copy of the mouse gene was inserted into the ldquokneerdquo of aDrosophila embryo the resulting fruit fly grew an eye on its leg The fly gene and themouse gene are similar enough to trade places and still functionmdasheven though theycome from animals that have not shared a common ancestor in at least 600 millionyears
Prentice Hall Biology
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12ndash5 Gene Regulation
1 Key Concept How is the lac operon regulated 2 Key Concept Describe how most eukaryotic genes are controlled 3 What genes control cell differentiation during development 4 What is a promoter 5 Critical Thinking Comparing and Contrasting How is the way hox genes are expressed in mice similar to
the way they are expressed in fruit flies How is it different
Making an AnalogyMake an analogy to demonstrate the different components of the lac operon Then explain in a short paragraphmdashusing your analogymdashhow the lac operon works
Prentice Hall Biology
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Chapter 12 DNA and RNA
Click on a Key Concept to link to the page where the concept is explained
12ndash1 DNA Key Concepts
Avery and other scientists discovered that DNA isthe nucleic acid that stores and transmits thegenetic information from one generation of anorganism to the nextHershey and Chase concluded that the geneticmaterial of the bacteriophage was DNAWatson and Cricks model of DNA was a doublehelix in which two strands were wound around eachother
Vocabulary
transformation bacteriophage nucleotide base pairing
12ndash2 Chromosomes and DNA Replication Key Concept
During DNA replication the DNA moleculeseparates into two strands then produces two newcomplementary strands following the rules of basepairing Each strand of the double helix of DNAserves as a template or model for the new strand
Vocabulary
chromatin histone replication DNA polymerase
12ndash3 RNA and Protein Synthesis Key Concepts
There are three main types of RNA messenger
Vocabulary
gene messenger RNA (mRNA)
Prentice Hall Biology
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Thinking Visually Protein Synthesis Flowchart
RNA ribosomal RNA and transfer RNADuring transcription RNA polymerase binds to DNAand separates the DNA strands RNA polymerasethen uses one strand of DNA as a template fromwhich nucleotides are assembled into a strand ofRNADuring translation the cell uses information frommessenger RNA to produce proteins
ribosomal RNA (rRNA) transfer RNA (tRNA) transcription RNA polymerase promoter intron exon codon translation anticodon
12ndash4 Mutations Key Concept
Mutations are changes in genetic material Genemutations result from changes in a single geneChromosomal mutations involve changes in wholechromosomes
Vocabulary
mutation point mutation frameshift mutation polyploidy
12ndash5 Gene Regulation Key Concepts
The lac genes are turned off by repressors andturned on by the presence of lactoseMost eukaryotic genes are controlled individuallyand have regulatory sequences that are much morecomplex than those of the lac operon
Vocabulary
operon operator differentiation hox genes
Prentice Hall Biology
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Modeling DNA ReplicationLiving cells synthesize exact copies of DNA molecules that are passed on to each daughter cell during celldivision In this investigation you will model DNA replication
Problem How is DNA replicated
Materials construction paper (tan gray green yellow red and purple)metric rulerscissorstransparent tape
Skills Using Models
Procedure
1 Cut out rectangles of construction paper in the sizes and colors indicated belowSugars 36 tan pieces each 2 cm times 2 cmPhosphates 36 gray pieces each 1 cm times 2 cmAdenines (A) 12 green pieces each 1 cm times 2 cmThymines (T) 12 yellow pieces each 1 cm times 2 cmGuanines (G) 6 red pieces each 1 cm times 2 cmCytosines (C) 6 purple pieces each 1 cm times 2 cm
2 To model a nucleotide tape together a phosphate group a sugar and a guanine molecule (G) (see DNANucleotides)
3 Assemble eight additional nucleotide models with the following nitrogenous bases3 thymines (T) 3 adenines (A) 2 cytosines (C)
4 To model a single strand of DNA tape the sugar of each nucleotide to the phosphate group of the nextnucleotide in the following order G T T A C A A T C
5 Construct a strand of DNA that is complementary to the first strand Tape the nucleotides of the secondstrand together as you did in step 4 Record the positions of the bases in both strands of your model
Prentice Hall Biology
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6 Place the two strands side by side so that their complementary nucleotides face each other Do not tape thetwo strands together Write ldquooriginalrdquo on each strand
7 Separate the two strands Simulate the action of DNA polymerase by constructing a new complementarystrand for each original strand
8 Tape the bases of each new strand to the complementary bases of its matching strand
Analyze and Conclude 1 Comparing and Contrasting Compare the new double-stranded DNA models with your original DNA
model Are their nucleotide sequences identical2 Using Models After a cells DNA is replicated the cell may divide in two Each new cell receives one
copy of the original cells DNA According to your model how are the new strands and the originalstrands divided between the two new cells
3 Drawing Conclusions What problems would you expect to occur if DNA was not copied accurately as itis replicated
4 Evaluating Do you consider this procedure an adequate model of DNA replication Explain your answer5 Describe an alternative way of modeling DNA replication
Using Models Cells use the information in DNA to make proteins First part of the nucleotide sequence ofDNA is copied into a complementary sequence of RNA in the process of transcription Then during translationthe cell uses information in the RNA to make proteins Modify your model or make a new model to show howtranscription and translation occur
Prentice Hall Biology Checkpoint
fileUsersesktopBiologyPH20BIO20Interactive20TextiTextproducts0-13-115516-4ch12cp_imcB04cp0289html[21917 110827 AM]
A virus that infects and kills bacteria is known as a(an)viral genebacteriumbacteriophage
Prentice Hall Biology Checkpoint
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The Hershey-Chase experiment showed that the part of the virus that entered the bacteria was thetailDNA coreprotein coat
Prentice Hall Biology Checkpoint
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The technique Franklin used to study DNA wasX-ray diffractionradioactive markerstransformation
Prentice Hall Biology Checkpoint
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Tightly packed DNA and proteins formnucleosomeschromatinhistones
Prentice Hall Biology Checkpoint
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Ribosomes are made of ribosomal RNA andproteinstransfer RNAmessenger RNA
Prentice Hall Biology Checkpoint
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Intervening sequences of DNA are known asexonscodonsintrons
Prentice Hall Biology Checkpoint
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The function of the operator region is toserve as a binding site of DNA-binding proteinsturn off repressorsturn on repressors
Prentice Hall Biology Checkpoint
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Nucleotides are joined together to form the DNA chain by links betweendeoxyribose molecules and phosphate groupsadenine and thymine nitrogenous basesphosphate groups and guanine nitrogenous bases
Prentice Hall Biology Checkpoint
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The organism with the highest percentage of adenine isyeasthumanherring
Prentice Hall Biology Checkpoint
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If the introns are not removed from the pre-mRNA the resulting protein would bemade correctlymade incorrectlynot made at all
Prentice Hall Biology Checkpoint
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The three-letter groups of the two codons shown here areTAC and TTGAUG and AACGUA and CAA
Prentice Hall Biology Checkpoint
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Which is an RNA polymerase binding site on a typical geneoperonpromoterrepressor
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Which section of the bodies of the flies and mice is coded by the genes shown in bluefrontrearcenter
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DNA Replication During DNA replication the DNA molecule produces two new complementary strands following the rules ofbase pairing Each strand of the double helix of DNA serves as a template for the new strand