Special Abstracts / Journal of Biotechnology 150S (2010) S1–S576 S431

blood and fat tissue, etc. This study aimed to assess the applicabilityof stem cells from different sources for their chondrogenesis poten-tial on collagen II scaffolds. We found that mesenchymal stem cellsfrom amniotic fluid have best chondrognesis potential in serum-free medium without TGF, and we also confirmed that those stemcell were induced to condrocyte-like cells on collagen II scaffold.

doi:10.1016/j.jbiotec.2010.09.604

[P-M.17]

Changes in skeletal muscle proteins in obesity-prone andobesity-resistance rats induced by high-fat diet

D.H. Kim ∗, J.W. Choi, J.I. Joo, J.W Yun

Daegu University, Republic of KoreaKeywords: Obesity; Skeletal muscle; High fat diet

With the developed lifestyle, availability of excessive high-calorie foods was increased that results in the escalation in theprevalence of obesity. For understanding of complicated pathogen-esis of obesity, high-fat (HF) diets-induced rodent model is widelyused. However, previous studies have shown differences in suscep-tibility to HF diet-induced obesity in rodents in spite of same geneticbackground. Distinction between susceptibility and resistance ofobesity can be identified at various genetic, physiologic, metabolic,and behavioral levels. In present study, we examined the differ-ential expression in skeletal muscle proteome using a proteomicapproach to search for novel biomarkers between susceptibilityand resistance to HF diet. Male Sprague-Dawley (SD) rats were feda general chow (n = 6) or HF diet (45% calories from fat) for 8 weeks,and then were classified obesity-prone (OP) (n = 7) and obesity-resistant (OR) (n = 7) rats by body weight. Consequently, OP ratsgained about 25% more body weight than OR rats, even though foodintake was not significantly different between two groups. In addi-tion, plasma leptin and insulin levels were slightly increased in theOP rats compared with those in OR rats. Differential muscle proteinsin OP and OR rats were investigated by proteomic and immunoblotanalysis. The proteomic analysis using 2-DE demonstrated that 23spots were differentially significantly expressed from a total ofabout 500 matched spots, of which 21 spots were identified asskeletal muscle proteins altered between OP and OR rats, and 2spots were found to be unidentified by mass fingerprinting. On theother hand, we also analyzed the differential change of gastroc-nemius muscle transcriptome using oligonucleotide microarrayanalysis in order to compare our proteomic data. Accordingly, wefound novel biomarkers of obesity using proteomic and transcrip-tomic analysis and these findings are the first report that addressesexpression proteomics dealing with muscle protein in OP and ORrats.

doi:10.1016/j.jbiotec.2010.09.605

[P-M.18]

Proteomic analysis for adipocytes in obesity-prone and obesity-resistance rats

Jeong In Joo ∗, Jung Won Choi, Jong Won Yun

Department of Biotechnology, Daegu University, Daegu University,Kyungsan, Republic of KoreaKeywords: Obesity; Adipocyte; Proteomics; Transcriptomic

Diet-induced obesity (DIO) in rodents has been used as an ani-mal model to investigate the interaction between environment

Figure 1. (A) Growth of normal controls fed general chow and OP and OR rats fedHFD. Left figure represents the change of body weight which follows at time andright figure displays total weight gain of rats. (B) Adipose tissue weight of OP andOR rats. n = 6 per group; p < 0.01, p < 0.001, Error bars represent means ± s.e.m.

and genetic background such as outbred Sprague-Dawley (SD)rats. Interestingly, when outbred rats are placed on an energy-dense, high fat diet (HFD), there is a wide distribution in bodyweight gain; some of the animals become very obese (obesity-prone, OP), whereas others remain as lean as animals fed a low-fatdiet (obesity-resistant, OR). The aim of this research is to lookinto obesity-related biomarkers in adipocytes of OP and OR ratsusing proteomic approach. The 5-weeks-old male SD rats weighing130-150 g were allowed to acclimatize for 1 week before exper-iments and individually housed on a standard chow diet or HFDcontaining 45% fat-derived calories. After 8 weeks, HFD rats weresubdivided into OP and OR rats according to body weight. Conse-quently, the mean body weight, plasma leptin and insulin levelsof OP rats were significantly higher than OR rats. And OP ratsshowed significantly increased weights of brown adipose tissue(BAT) and white adipose tissue (WAT) compare with OR rats. Weperformed differential proteomic analysis using 2-DE combinedwith MALDI-TOF mass spectrometry to elucidate the molecularaction of differential proteins in OR compared with OP rats inepididymal WAT and interscapular BAT. In addition, we also per-formed transcriptomic analysis in BAT and WAT of OP and OR ratsusing oligonucleotide microarray technique. Through these variousbiological methods, we discovered adiopocyte specific-differentialproteins related obesity. These results suggest that these differen-tial proteins could causes of phenotypic distinction between OPand OR rats. Our findings are the original report that demonstratesbiomarkers in adipocyte of OP and OR rats by proteomics and tran-scriptomic approaches. Fig. 1.

doi:10.1016/j.jbiotec.2010.09.606

[P-M.19]

Large generation and characterization of functional dendriticcells from serum free expanded human cord blood CD34+ cells

Chao-Ling Yao 1,2,∗, Yu-Hong Wei 1,2

1 Department of Chemical Engineering and Materials Science, Yuan ZeUniversity, Chung-Li, Taoyuan, Taiwan, Taiwan2 Graduate School of Biotechnology and Bioengineering, Yuan Ze Uni-versity, Chung-Li, Taoyuan, Taiwan, TaiwanKeywords: Serum-free medium; Hematopoietic stem cells

The current therapies for cancer patients, including surgicaloperation, chemotherapy, or radiotherapy, were always followedwith heavy side affect, and had high possibility to relapse. In human,