Central Instrumentation Laboratory

Mission Statement

CIL aims at the optimized utilization of the instrumentation facility with quality assurance of

analytical and subsequent research outcomes.

Role of CIL in the University

Central Instrumentation Laboratory (CIL) has been incepted in the University in 2010. An

amount of Rs. 7 crores has been spent to house 8 state-of-the art instruments of common use

type. It has been playing a pivotal role in supporting the research activities of the six Schools in

Science and Technology and Twelve Centres distributed among these schools.

Facilities in the Laboratory:

• The central instrumentation laboratory presently hosts the following analytical

instruments

– Gas Chromatography-Mass Spectrometer

– Atomic Absorption Spectrometer

– Ultra High Performance Liquid Chromatography

– UV-Visible Spectrometer

– Fourier Transform Infrared Spectrometer

• Apart from analytical instruments, laboratory also hosts an imaging section for basic and

applied research in biological and material. The imaging instruments include

– Fluorescence Microscope

– Field Emission Scanning Electron Microscope

– Confocal Laser Scanning Microscope

OBJECTIVES

I. To provide basic research support to the centres of science and social science for their

instrumentation requirements.

II. To strengthen the facility with state-of-art advanced research instruments making it a

model hub for scientific research in this region.

III. Maintenance and upgrade of instruments in the facility as well as that at various centres

in the university.

IV. Promoting University and Industry Collaboration through essential Services for

sponsored research and consultancy.

Research support

The university has a high proportion of research students in the sciences and an active faculty

of 25 in the science disciplines. The CIL is playing a vital role in meeting their equipment needs

and this aspect is particularly appreciated by the newly joined faculties, who necessarily have to

spend a few years initially in developing their research laboratories. CIL has been providing a

major support to all the science schools with an average number of around 10 common-use

type of state-of-the-art instruments covering microscopy, spectroscopy, and chromatography.

Within this small span of four years of its inception, the CIL has achieved an average of 7022

samples run every year with a highest amount of 10,880 samples taken up and completed in

year 2014. With the increase in demand due to the growing faculty strengths of science schools

the year 2014 has accounted for 10,880 samples studied.

Instruments with Specific Precautions:

1. The GC-MS facility is equipped with radioactive nuclide 63Ni. It is a potent radiation

source.

2. The confocal facility is equipped with Class 3B lasers. Direct contact of lasers may

damage the exposed parts of the body.

Support Facility

1. Uninterrupted Power Supply: Capacity upto 60 KVA for backup of Atomic Absorption

Spectrometer, Gas and High Pressure Liquid Chromatography, Mass spectrometry,

Fluorescence, FE-SEM and Confocal Microscopy.

2. Online Backup Genset

3. Dedicated Earthings <1 Volt

4. Modular Laboratory Furniture

5. Gas Equipment Assembly

Hands-on Training Programs and Sessions Conducted:

1. Training for Confocal Laser Scanning Microscope on 28-29th May, 2014 for nominated

faculties Dr. Monisha Dhiman, Dr. Vikas Jaitak.

2. Training Program on Fluorescence Correlation Spectroscopy on 26th June, 2014.

Introduction to the FCS system by Dr. Volker Buschmann, Sr. Application Scientist,

Picoquant GMBH.

3. Talk on Sample Preparation and Experiments on Confocal Laser Scanning Microscopy,

26th June, 2014 by Dr. K. N. Ganesh, Olympus India Pvt. Ltd.

4. Training on Field Emission Scanning Electron Microscopy (FE-SEM) Held on August,

2014 for nominated faculties from each centre in science by Alok Tripathi, Assistant

Manager, Services Carl-Ziess India Pvt. Ltd.

Several of the internal users have acknowledged the services rendered by the CIL in their

theses/dissertations/research publications.

Instruments in Central Instrumentation Laboratory

Spectrometric Instruments

MicroscopyInstruments

Central Instrumentati

on Laboratory

Chromatographyic

Instruments

• Field Emission Scanning Electron Microscope (FE-SEM)

• Confocal Laser Scanning Microscope with Fluorescence Correlation Spectroscopy

• Fluorescence Microscope

• UV-Visible Spectrophotometer • Fourier Transform Infrared (FTIR)

Spectrometer • Atomic Absorption Spectrophotometer

(AAS) • Flow Cytometer (Fluorescence Activated

Cell Cytometer)

• Gas Chromatography – Mass Spectrometry

• Gas Chromatography with FID/TCD

• Ultrahigh Performance Liquid Chromatography (UPLC)

UV-Visible Spectrophotometer

Make: Shimadzu, Japan

Model: UV-2450

Date of Installation: 03/04/2012

It is an instrument of basic need in research including life sciences and common

analysis. The instrument features an adjustable slit width and features analytical

measurements like full spectrum, Absorbance and kinetic modes.

The spectrometer host the following for the analysis:

Double and Multi-cell Holder

Flow Cell Assembly

Peltier Cooled Thermostat

Fourier Transform Infrared Spectrometer

Make: Bruker

Model: Tensor 27

Date of Installation: 05/09/2012

The Fourier transform infrared spectrometer can perform functional group analysis of solids, thin-films and liquids. The system consists of:

ZnSe Beam Splitter

KBr Pellet Accessories

NaCl Cell for Liquid

Attenuated Total Reflectance (ATR)

Diffused Reflectance (DRIFT)

Atomic Absorption Spectrometer

Make: Shimadzu, Japan Model: Shimadzu AA 7000 Date of Installation: 26/12/ 2012

Atomic Absorption Spectrometer is equipped with the latest techniques of flame hydride

and furnace analysis of inorganics in solution. The AAS features an automated switch

over from flame to furnace, which is desired for ultra-trace level analysis of metals upto

parts per billion. The equipment is presently equipped with hollow Cathode lamps for

Analysis of Eleven metal ions. Another, 17 Hollow Cathode lamps have been ordered

for in the present financial year.

The present AAS facility consists of

Flame Adsorption

Hydride Generator

Graphite furnace

The system is well equipped for Analysis of following metals

1) Sodium (Na) 2) Manganese (Mn) 3) Barium (Ba)

4) Selenium (Se) 5) Aluminium (Al) 6) Indium (In)

7) Arsenic (As) 8) Beryllium (Be) 9) Lithium (Li)

10) Chromium(Cr) 11) Cadmium (Cd) 12) Molybednum (Mo)

13) Magnesium (Mg) 14) Zinc (Zn) 15) Strontium (Sr)

16) Mercury (Hg) 17) Nickel (Ni) 18) Silicon (Si)

19) Iron (Fe) 20) Lead (Pb) 21) Vanadium (V)

22) Cobalt (Co) 23) Boron (B) 24) Thallium (Th)

25) Copper (Cu) 26) Antimony (Sb) 27) Tin (Sn)

28) Tellurium (Te)

in purchase process

Flow Cytometer

Make: BD Bioscience Model: Accuri C6 Date of Installation: 21/12/2015

The present system is reliable for accurate and sensitive analysis of fluorescence in cell

cultures.

Gas Chromatography- Mass Spectrometer

Make: Shimadzu, Japan Model: Shimadzu QP-2010 Ultra GC-MS Date of Installation: 20/01/2014

The system is dedicatedly used for natural product, essential oil, bio-oil and pesticide

residue analysis. The system is already being used for the qualitative and quantitative

analysis of organophosphorus pesticides and Polynuclear Aromatic Hydrocarbon (PAH)

residues in environmental compartments (Water, Soil and Air). Apart from this, new

methods are being developed for volatile and semi-volatile organics using the

Headspace analysis. Comprehensive quantitative analysis of Organochlorine Persistent

Organic Pollutants (POPs) could be carried out upto a sensitivity of 0.001 ppb

concentration.

The present GC-MS facility consists of

PTV Injector

Auto-sampler

MS-Detector EI/CI

ECD

FID

TELEDYNE TEKMAR HT3 Headspace sampler

Capillary Columns

DB-5

DB-1

Carbowax

The system is well equipped for pesticide residue analysis.

Gas Chromatography- TCD/FID

Make: Nucon, Delhi

Model: NUCON 5700 Date of Installation: 02/09/2013

The system is equipped for assembling both the wide bore columns (Glass and Steel)

as well as Capillary Columns. The system provides the analysis of purity assay,

bioethanol, bio-diesel, producer gas and biogas analysis.

The system comprises of

Data Workstation with Two channels

Thermal Conductivity Detector (TCD)

Flame Ionization Detector (FID)

Gas collection devices for analysis

Gas Calibration Standards

This instrument with Gas Chromatography-Mass Spectrometer completes the volatile

organic analysis with all the universal detectors equipped on both the systems.

Ultra High Performance Liquid Chromatography (UPLC)

Make: Thermo Scientific Model: Dionex Ultimate 3000 Date of Installation: 11/11/2011

The UPLC system is equipped for routine chromatographic analysis of pesticides,

carbohydrates and small organic drugs etc.

The facility is of following configuration:

Quaternary Solvent

Auto Sampler

Auto Sampler

DAD Detector

FLD Detector

RI Detector

Thermostatted Column Compartment

Columns: C-18, C-16 and C-8

Field Emission Scanning Electron Microscope

Make: Carl Zeiss, Germany

Model: Merlin Compact

Date of Installation: 28/07/2014 The Field emission scanning electron microscope has resolution of upto 1.0 nm at a

working distance of 4 mm and acceleration voltage of 30 KV and 1.6 nm at 1 KV. The

working distance of the system could be varied between 2.5 to 44 mm and acceleration

voltage between 0.02 V to 30 KV, which provides flexibility for analysis of the sample

with better imaging resolution. The system has a magnification of upto 2,000,000.

The system is equipped with six detectors which make the system comprehensive.

Backscattered Electron Detector(BsED)

Secondary Electron Detector (SED)

STEM Detector

Inlens Detector

TV Mode

Oxford EDX Detector

Gold/Platinum Sputtering/Coating

Confocal Laser Scanning Microscope

Make: Olympus, Japan

Model: Olympus FV1200 CLSM

Date of Installation: 28/05/2014

It is one of the most advanced type of Confocal Microscopy systems, highly sensitive even in low signals. The system has a resolution of upto 200 nm.

The system comprises of following:

Olympus IX-83 Microscope FV-1200 Scanner Mercury arc lamp and Six laser lines of 405, 458, 488, 515, 559 and 635

nm wavelengths. Picoquant’s FCS attachment for Fluorescence correlation studies. Oko Labs’ Live cell imaging set up

The system is efficiently able to perform following type of experiments:

Multichannel Imaging with Z-stack Co-localization studies Time lapse experiments FRET

Fluorescence Microscope

Make: Olympus, Japan

Model: FSX-100 Bioimaging Navigator

Date of Installation: 10/06/2010

This system is compact, easy to operate and comprehensive.

System is well suited for following applications

Bright field Imaging

Phase Contrast Imaging

Multicolour Fluorescence Imaging

Z-stack Imaging

Time lapse Experiments (Live cell Imaging)