cell culture brochure - bd

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BD Falcon™ Cell Culture Products

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Trust BD Falcon,™the first name in cell culture

As the first company to produce sterile, disposablelabware more than 50 years ago, BD Biosciences is aworldwide leader in top-quality cell culture products.If you’re looking for reliability, consistency, and convenience, trust the company with a reputationfor delivering quality.

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Unique Surface Chemistry for Enhanced Cell CultureConsistent cell culture conditions arerequired for reproducible researchresults. In the manufacture of all cellcultureware, hydrophobic polystyreneis permanently rendered hydrophilicto support cell attachment andspreading.1,2,3 The consistency of thissurface depends on the treatmentmethod used.

Many manufacturers have long usedatmospheric plasma treatments (i.e.,corona) to create hydrophilic surfaces.In corona treatment, a high-voltagedischarge creates a reactive gas plasmaabove the growth surface of the vessel.In this process, the highly interactivegas plasma mixture is exposed toambient air. The consistency of the treatment surface can therefore be compromised by day-to-dayenvironmental changes.

At BD Biosciences, molded polystyrenevessels are placed in a closed, highlycontrolled environment that preventscontamination and ensures a consistenttreatment surface.

BD Biosciences made a significantresearch investment to develop thisunique vacuum-gas plasma processused to produce both BD Primaria™

and traditional tissue culture (TC)surfaces on BD Falcon™ dishes,plates, flasks, and roller bottles.

The incorporation of nitrogen-containing cations has been correlatedto attachment and spreading of primaryendothelial cells in a clonal cell-growth assay.4 The complex surfaceon BD Primaria cultureware ishomogeneous and stable and has beenin use by researchers for over a decade toimprove attachment and differentiationof a variety of cell types. For example,cell biologists have used BD Primariafor cultivating hepatocytes,5,6 neuronalcells7, and other endothelial cells.8

The surface chemistry of BD Primariaproducts has been confirmed byElectron Scanning for ChemicalAnalysis (ESCA).

BD Biosciences’ cell culture productsmeet high quality control standardsand are manufactured under theregistered ISO 9000 series QualitySystems. A sensitive clonogenic assayis used to validate the specificconditions used to treat each new TCproduct. Routine, 72-hour confluencytesting ensures lot-to-lot quality. Ourvacuum-gas plasma treatment processmakes BD Falcon Cell Culturewareyour best choice for confidence in theconsistency of your cell culture condi-tions.

References

1. Ertel, S., et al., Endothelial cell growth onoxygen-containing films deposited by radio-frequency plasmas; the role of surface car-bonyl groups. Biomater. Sci.: Polym. Ed.3:163 (1991).

2. Curtis, A.S.G., et al., Substrate hydroxylationand cell adhesion. J. Cell Sci. 86:9 (1986).

3. Ramsey, W.S., et al., Surface treatments andcell attachment. In Vitro 20:802 (1984).

4. Chilkoti, A., et al., Investigating the relation-ship between surface chemistry and endothe-lial cell growth: partial least-squares regres-sion of the static secondary ion mass spectraof oxygen-containing plasma-deposited films.Analytical Chemistry 67:2883 (1995).

5. Boisclair, Y.R., et al., Role of the Suppressorof Cytokine Signaling-3 in Mediating theInhibitory Effects of Interleukin-1ß on theGrowth Hormone-dependent Transcriptionof the Acid-labile Subunit Gene in LiverCells. J. Biol. Chem. 275(6):3841 (2000).

6. Braun, J.R., et al., The Major Subunit of theAsialoglycoprotein Receptor Is Expressed onthe Hepatocellular Surface in Mice Lackingthe Minor Receptor Subunit. J. Biol. Chem.271(35):21160 (1996).

7. Holgado-Madruga, M., et al., Grb2-associatedbinder-1 mediates phosphatidylinositol 3-kinase activation and the promotion of cellsurvival by nerve growth factor. PNAS USA94:12419 (1997).

8. Silverman, D.J., et al., Primary isolation ofhuman umbilical vein endothelial cells on asurface-modified tissue culture dish. BDLMonograph (1986).

Additional References

Chat, G. and Coca-Prados, M., Comparison oftissue culture systems for the growth of ciliaryepithelial cells. BDL Monograph (1986).

Chinn, J.A., et al., Laboratory preparation ofplasticware to support cell culture. J. TissueCulture Methods 16:155 (1994).

Traditional Tissue Culture

BD Primaria

Traditional Tissue Culture BD Primaria™

When chick embryo spinal cord neurons are cultured on BD Primaria™, growth is enhancedand extensive neurite developmentoccurs (above, right). In this experi-ment, cells clumped and detachedfrom traditional TC plates (above,left) after 20 days in culture, butremained viable and differentiatedon BD Primaria.Note: At pH 7, carboxy groups may be slightlydissociated and assume a negative (anionic)charge. Amine groups may protonate andassume a positive charge (cationic).

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BD Falcon™ Flasksfeature various neckdesigns, shapes, andcap styles to meet your application needs.

What makes the BD Falcon™ CellCulture Flasks stand out from thecompetition is not only its surfacetreatment, but also the TC-friendlypackaging, variety of flasks, andassortment of caps.

When BD Biosciences decided toredesign its flask packaging, a specialtask force was appointed to determinewhat customers valued most. The teamvisited stockrooms, distributor ware-houses, and research laboratories toidentify customer concerns and priori-ties. Several prototype packages weredeveloped and reviewed by numerouscustomers in seven countries, which ledto further refinements and modifica-tions. This process provided the infor-mation needed to deliver innovativepackaging that meets our customers’needs.

• Convenient reseal tab on the bag

• Innovative bag materials that willnot scuff or scratch the flask’s opticalsurface

• Double-wall bags for increasedsterility assurance

• Medical-style, peel-open bags forguaranteed flask sterility

• Recyclable bag material (LDPE)

• Easy-open case—no knife required

The innovative shape of theBD Falcon low-profile flaskallows access to all corners.BD Biosciences offers a low-profile,150 cm2 flask for efficient stackingand incubator utilization. Ourpatented locking incubation positionprevents caps from falling off or clos-ing while in the open position. Theinnovative shape of our low-profileflasks permits access to all cornerswith a pipet or scraper.

Choose from three easy on/off cap stylesBD Biosciences offers several easyon/off cap styles suited to your appli-cation. Our industry-standard plug-seal caps provide a liquid-tight sealwhen closed and an open incubationposition for reliable gas exchangewhen partially opened. Our ventedcaps allow consistent gas exchangewhen the caps are in the closed posi-tion. Gases required for cell growthand metabolism pass freely throughthe vent while microorganisms cannot.Lined, phenolic caps are available forlong-term cultures. The rigid capmaterial allows the cap to be easilyspun onto the neck during repetitivemanipulations. Our white caps identifynon-TC-treated flasks.

Cell Culture Flasks

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BD Falcon™ 175 cm2 Bar-Coded Flaskscertified for use in The AutomationPartnership’s SelecT ™ and CompacT SelecT ™

automated cell culture systems.

Plug-seal, vented, and phenolic caps

BD Falcon™ Cell Culture Flasks

12.5 cm2 Canted NeckTotal volume: 25 ml; Qty./bag: 10

Growth Surface Cap Style Qty./Case Cat. No.

Standard TC Plug-seal 100 353018Standard TC Vented 100 353107

25 cm2 Canted NeckTotal volume: 50 ml; Qty./bag: 20


Standard TC Plug-seal 200 353014Standard TC Vented 100 353108Primaria TC Plug-seal 200 353813Primaria TC Vented 100 353808



Standard TC Plug-seal 200 353082Standard TC Vented 100 353109Standard TC Phenolic 200 353081Non-treated Plug-seal 200 353009

75 cm2 Straight NeckTotal volume: 250 ml; Qty./bag: 5


Standard TC Plug-seal 100 353024Standard TC Vented 100 353110Standard TC Phenolic 100 353023BD Primaria TC Plug-seal 100 353824BD Primaria TC Vented 100 353810

75 cm2 Canted NeckTotal volume: 250 ml; Qty/bag: 5


Standard TC Plug-seal 60 353135Standard TC Vented 60 353136Standard TC Phenolic 60 353134Non-treated Plug-seal 60 353133






Standard TC Plug-seal 40 353028Standard TC Vented 40 353112Standard TC Phenolic 40 353045Standard TC Vented, 40 353118

Bar coded







Cell Culture Flasks

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BD Falcon™ Plates feature unique surfacechemistries for better cell attachment.

Cell Culture Plates

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6-well flat-bottom with LidGrowth area: 9.6 cm2; Well volume: 15.5 ml

Growth Surface Qty./Pkg Qty./Case Cat. No.

Standard TC 1/tray 50 353046Standard TC 6/bag 36 353224Standard TC 10/RS tray 60 353934Primaria TC 1/tray 50 353846Non-treated 1/tray 50 351146


Growth Surface Qty./Pkg Qty./Case Cat. No.

Standard TC 1/tray 50 353043Standard TC 6/bag 36 353225Non-treated 1/tray 50 351143


Growth Surface Qty./Pkg. Qty./Case Cat. No.

Standard TC 1/tray 50 353047Standard TC 6/bag 36 353226Standard TC 10/RS tray 60 353935Primaria TC 1/tray 50 353847Non-treated 1/tray 50 351147



Standard TC 1/tray 50 353078Standard TC 6/bag 36 353230Non-treated 1/tray 50 351178



Standard TC 1/tray 50 353072Standard TC 5/bag 50 353075Standard TC bulk 100 353916Standard TC 14/RS tray 84 353936Primaria TC 1/tray 50 353872Non-treated 1/tray 50 351172

96-well flat-bottom without LidGrowth area: 0.32 cm2; Well volume: 0.37 ml


Standard TC 1/tray 50 353070

96-well U-bottom with LidGrowth area: 0.36 cm2; Well volume: 0.32 ml


Standard TC 1/tray 50 353077Standard TC 5/bag 50 353227Standard TC bulk 100 353917Non-treated 1/tray 50 351177

96-well U-bottom without LidGrowth area: 0.36 cm2; Well volume: 0.32 ml


Standard TC 1/tray 50 353076

96-well LidQty./Pkg. Qty./Case Cat. No.

Lid, sterile 1/bag 50 353071



Standard TC 25/pkg 100 353289Standard TC 5/pkg 50 353961

384-well black/clear with LidGrowth area: 0.32 cm2; Well volume: 0.37 ml

Growth Surface Qty./Pkg. Qty/Case Cat. No.

Standard TC 25/pkg 100 353280Standard TC 20/pkg 180 353221Standard TC 5/pkg 50 353962

384-well black/clear without LidGrowth area: 0.32 cm2; Well volume: 0.37 ml


Standard TC 25/pkg 100 353298

384-well white/clear with LidGrowth area: 0.32 cm2; Well volume: 0.37 ml



384-well white with LidGrowth area: 0.32 cm2; Well volume: 0.37 ml



Cell Culture Plates

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Serrated gripping panels facilitate easy handling.

An innovative, labyrinth air passagesystem provides a tortuous path for gasexchange across BD Falcon plates. Thispatented feature reduces evaporationand minimizes contamination.

Low-Evaporation Lid

BD Falcon™ Cell CulturePlatesBD Biosciences uses a well-characterized,crystal-grade polystyrene and a highlycontrolled vacuum-gas plasma processto create the consistent growth surfaceson BD Falcon™ Cell Culture Plates.

Our extensive line of multiwell plates feature a patented labryinth lid, condensation rings, and deep-welldesign to control contamination whilereducing evaporation and minimizingedge effects. The plates feature serratedgripping panels for easier handling anda writing patch for plate identification.Our plates are available non-treated,TC-treated, or with our exclusive BD Primaria™ treatment to meet all your application needs.

BD Falcon plates are supplied inconvenient, medical-style packagingfor sterile product presentation. BD Falcon Ready-Stack (RS) traypackaging is designed for higherthroughput applications.

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Choose from an assortment of exceptionallyflat dishes for distortion-free optics.

Cell Culture Dishes

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BD Falcon™ Cell CultureDishesOur extensive line of non-pyrogenicBD Falcon™ Cell Culture Dishes areavailable with standard TC treatmentor BD Primaria™ to support cellattachment and spreading. Vacuum-gas plasma treatment permanently and consistently modifies the cellgrowth surface for uniform chemistryand attachment.

BD Falcon Dishes are exceptionallyflat for distortion-free optics. The dish lids are designed for optimal gasexchange. Stacking rings on the dishesallow for easier stacking and handling.Sterilized by gamma irradiation, thecrystal-grade polystyrene dishes arepackaged in peel-open, medical-style bags.

Our easy-grip dishes improve handling.The unique design and frosted rimfound on BD Falcon Easy-Grip Dishesimprove handling. The ability to pickup a small dish conveniently withoutaccidentally removing the lid allowsyou to work faster and improves aseptic manipulation.

A sensitive clonogenic assay usingMRC-5 cells, a diploid human fibro-blast line, is used to validate the man-ufacturing process for each BD FalconTC product. Routine testing of standardTC products is performed by testinggrowth to confluency at 72 hours withMRC-5 or WI-38 cells, a diploid humanlung fibroblast cell line. The surfacechemistry of each lot of BD Primariaproducts is confirmed by ESCA.

BD Falcon™ Easy-Grip Dishes

Each BD Falcon tissue culture product isdeveloped using a sensitive clonogenicassay.1 Shown here is a 35 mm dish withMRC-5 cells stained with crystal violet.

Reference1. Freshney, R.I., Culture of animal cells:

a manual of basic technique, 2d ed., Wiley-Liss, London, p. 83 (1987).

TC Process Validation

35 x 10 mm Style Easy-Grip DishesActual dimensions: 40.28 mm O.D. x 6.17 mmActual growth area: 11.78 cm2

Working volume: 2.5-3.0 ml

Growth Qty./ Qty./Surface Sleeve Case Cat. No.

Standard TC 20 500 353001Primaria TC 20 200 353801Non-treated 20 500 351008

60 x 15 mm Style Standard DishesActual dimensions: 54.81 mm O.D. x 13.26 mmActual growth area: 21.29 cm2



Standard TC 20 500 353002Primaria TC 20 200 353802IVF TC 20 500 353652Non-treated 20 500 351007

60 x 15 mm Style Easy-Grip DishActual dimensions: 52.10 mm O.D. x 13.13 mmGrowth area: 19.5 cm2



Standard TC 20 500 353004

100 x 20 mm Style Standard DishesActual dimensions: 89.43 mm O.D. x 19.18 mmActual growth area: 58.95 cm2



Standard TC 20 200 353003Primaria TC 20 200 353803Non-treated 20 200 351005

150 x 25 mm Style Gridded Dish(20 mm grid molded in base)

Actual dimensions: 142.57 mm O.D. x 24.77 mmActual growth area: 156.36 cm2



Standard TC 10 100 353025Non-treated 10 100 351013

60 x 15 mm Style Center-Well Organ Culture DishActual dimensions: 54.84 mm O.D. x 13.56 mmGrowth area in well: 2.89 cm2


Standard TC 20 500 353037IVF TC 20 500 353653

Cell Culture Dishes

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Bacteriological Petri Dishes

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BD Falcon™, the first manufacturer of disposablepetri dishes, continues to be the leading sourceof high-quality, research-grade petri dishes. Avariety of standard and specialty petri dishes areavailable to meet your application needs.

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Product Class

11

Bacteriological Petri Dishes

Unique Dish DesignsThe unique design and frosted rimfound on BD Falcon™ Easy-GripDishes improves the handling of smalldishes. The BD Falcon Tight-Fit LidDish minimizes sample dehydration.

BD Falcon™ Integrid DishesBD Falcon Integrid Dishes are availablein the large 150 mm size as well as thesquare 100 mm size. The molded-ingrid patterns on the outside bottom of the dishes facilitate locating andcounting colonies.

BD Falcon™ BioDish XLThe BD Falcon BioDish XL is a 245 x245 x 18 mm square Petri Dish for usein automated colony picking robots.An alphanumeric 5 x 7 gridded dishmatches the map commonly used incolony picking robots, providing avisual aid to colony locations. A bentorientation corner is a visual guide tocolony location. Horizontal lines atcommon fill volumes extend the lengthof the dish, facilitating even pouringof agar. Stacking rings protect theviewing surface and allow for easierhandling.

BD Falcon™

Compartment DishesThe BD Falcon Compartment Dishesallow differential studies of organismsor media in a common environment.The compartments enable minimalmedia use and conserve storage space.The dishes come in a variety of formatswith two, three, or four compart-ments. The numbered segments andthe triangular reference mark on theside wall of the dish bottom facilitateprocedures requiring an orientationpoint.

BD Falcon™ Bacteriological Petri DishesQty./Sleeve Qty./Case Cat. No.

35 x 10 mm Easy-Grip Dishes 20 500 351008Actual dimensions: 40.28 mm O.D. x 6.17 mm

60 x 15 mm Standard Dishes 20 500 351007Actual dimensions: 54.81 mm O.D. x 13.26 mm



100 x 20 mm Optilux™ Dishes 20 200 351005Actual dimensions: 89.43 mm O.D. x 19.18 mm

50 x 9 mm Tight-Fit Lid Dish 20 500 351006Actual dimensions: 50.25 mm O.D. x 8.26 mm

BD Falcon™ Integrid DishesQty./Sleeve Qty./Case Cat. No.

100 x 15 mm Square Dish 10 300 351112Actual dimensions: 90.50 mm O.D. x 15.24 mmGrid size: 13 mm150 x 25 mm Round Dish 10 100 351013Actual dimensions: 142.57 mm O.D. x 24.77 mmGrid size: 20 mm

BD Falcon™ BioDish XLQty./Sleeve Qty./Case Cat. No.

245 x 245 x18 mm Square Dish 5 20 351040Actual dimensions: 245 mm x 245 mm x 18 mm

BD Falcon™ Compartment DishesQty./Sleeve Qty./Case Cat. No.

100 x 15 mm I-Plate (two sections) 20 500 351003Actual dimensions: 86.30 mm O.D. x 12.70 mm

100 x 15 mm X-Plate (four sections) 20 500 351009Actual dimensions: 86.30 mm O.D. x 12.70 mm

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BD Falcon™

Roller Bottles aremanufactured ina class 100,000 clean room.

Roller Bottles

BD Falcon™ TufRol™

Roller BottlesThe BD Falcon™ TufRol™ RollerBottle has a one-piece design. Whilepolystyrene does not normally offerhigh-impact resistance, the TufRolbottle is manufactured using a propri-etary manufacturing process thatprovides excellent impact resistance.This inherent, high-strength featurereduces the risk of messy spills andloss of precious contents during acci-dental mishandling.

Roller bottles are used in both researchand manufacturing applicationsinvolving the scale-up of mammaliancells for purposes of virus propagationand bioproduct production. BD FalconRoller Bottles are tissue culture-treated,non-pyrogenic, and sterilized by gammairradiation. As with most BD Falconproducts, our Roller Bottles aremanufactured under a registered ISO 9002 Quality System.

Custom ProductsSince many industrial customers have special requirements relative to labeling, lot control, packagingconfigurations, and delivery schedules,we are able to develop custom servicesand products to suit individual needs.As with all BD Biosciences products,certification is available upon request.

Multiple Cap Styles andSurface TypesBD Falcon Roller Bottles are availablewith a smooth surface with a growtharea of 850 cm2 or a pleated surfacewith a growth area of 1450 cm2. Easy on/off or vented cap styles areavailable. The vented cap has anintegral hydrophobic 0.2 µm micro-porous membrane filter vent, allowingconsistent gas exchange when the capis in the closed position.

Automation-FriendlyOne-piece design of BD Falcon RollerBottles is compatible with manual orautomated filling systems. Our RollerBottles are supplied with easy on/offpolyethylene caps that are designed toprevent misthreading and to provide asecure seal.

BD Falcon™ TufRol™ RollerBottles

Smooth Surface Growth area: 850 cm2

Cap Qty./Bag Qty./Case Cat. No.

Easy on/off 2 20 353007Easy on/off 20 20 353008Easy on/off 20 20 353088

(double bagged)

Vented 2 20 353068

Pleated Surface Growth area: 1450 cm2


Easy on/off 20 20 353079Vented 20 20 353069

(double bagged)

CapsQty./Bag Qty./Case Cat. No.

Easy on/off 250 500 358027Vented 250 500 353101

BD Falcon™ TufRol™ EZ RollerBottles

Deep Indent Growth area: 850 cm2


EZ twist screw 2 20 353151EZ twist screw 20 20 353152EZ twist screw 20 20 353153

(double bagged)

EZ twist vented 2 20 353154

Shallow Indent Growth area: 850 cm2


EZ twist screw 2 20 353160EZ twist screw 20 20 353161

(double bagged)

EZ twist vented 2 20 353162

BD Falcon™ TufRol™ EZ Roller Bottles can be held from the bottom when pouring; a comfortableand convenient grip for many researchers.

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Roller Bottles

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BD Biosciences can custom coat roller bottles for specialized cell types. Please contact ourTechnical Services department at 877.232.8995 for custom samples and a quotation.

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BD Cell™ MAb Medium

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BD Cell™ is a new basal medium formu-lated to produce high-yield monoclonalantibody (MAb) secretion using serumor serum-free supplementation. Thissystem enables the purification of 5 to25 times more antibody compared toconventional media with similar sup-plementation. BD Cell also reducesmedia consumption and labor cost.

Benefits of BD Cell™ in Roller Bottle ProductionFirst evaluated in roller bottles, BD Cellhas proven itself a worthy alternativeto traditional basal media. While astandard method of culturing anti-body, roller bottle production hassome limitations: extensive labor andfrequent feedings. The initial rollerbottle evaluation compared BD CellMAb Medium (basal) with Iscove’sModified Dulbecco’s Medium (IMDM)both supplemented with 10 percentfetal bovine serum (FBS). The cultureswere inoculated at a density of 2 x 105

cells/ml. The IMDM was run conven-tionally with harvesting and feedingperformed every two to three days. By contrast, the BD Cell culture wasrun batch-fed and incubated untouchedfor 21 days. Now evaluated in a pro-duction environment on more than300 hybridoma lines, BD Cell’s averageincrease of antibody yield is 8 to 10times that of classic IMDM media.

In the biopharmaceutical industry,Bovine Serum Extract (BSE) concernshave resulted in the need to replacemedia that contain animal-derivedcomponents with a non-animal alter-native. The challenge has been tomake substitutions that do not adverselyaffect the performance of the cell lines.A selection of non-animal peptones was

evaluated in BD Cell MAb Medium tooptimize a cell culture medium thatwas free of animal components. Thesoy hydrolysate Select Soytone waschosen based on optimal performanceand was used to formulate a BD CellMAb Medium free of animal-basedcomponents.

Yie

ld (g

/ml)

ClonesRoller Bottle - 21 Day Run

XMG1.2

500

450

400

350

300

250

200

150

100

50

0UCHT1

BD Cell(w 10% FBS)

Control(IMDM w/10% FBS)

RA3-6B2 KAY-10

The BD Cell MAb Serum-Free Medium shows similar results when compared to IMDM withserum (data on file).

Yield Analysis of BD Cell UsingProtein-G Purification

BD Cell™ MAb MediumDescription Qty. Cat. No.

Basal Medium 1000 ml bottle 220511Serum-Free Medium 1000 ml bottle 220509Animal Component-Free Medium 1000 ml bottle 220513

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Product Class

15

CELLine™ System

CELLine™ System: CultivationFlask for Secreted ProductThe CELLine™ 1000 Device, based onmembrane technology, is a new devel-opment in cell cultivation. The systemguarantees high cell densities and iseasy to use for recombinant proteinexpression and high yield monoclonalantibody production.

Monoclonal antibodies (MAbs) arebecoming increasingly important asresearch tools. The primary methodsavailable to generate research quanti-ties of MAbs (10 mg to 500 mg) arestatic tissue culture, spinner, or rollersystems and ascites fluid from mice.As demand for MAbs has increased,so has the pressure to develop alterna-tive in vitro production methods thatwill reduce animal use, streamlinedownstream processing, and reducevariability in production runs. Theeffort to develop a production methodthat meets these emerging require-ments has culminated in the CELLineSystem, a novel, membrane-based dis-posable cell cultivation system.

The CELLine System yields antibodyconcentrations comparable to that ofascites. One CELLine flask can be usedto produce as much antibody as thatof 12 mice. The harvest volumes alsoresult in antibody concentrations thatare 50 to 100 times higher than bothroller bottles and tissue culture flasks.Average yields of monoclonal antibod-ies range from 1 to 5 mg/ml. Using BDCell MAb media in a CELLine device,antibody yields average between 30 to150 mg of antibody every two weeks.

Description Qty Cat. No.

CELLine CL-1000 3 flasks 353137

0 5 10 15 20

BD Cell

RPMI

Days in Culture

IgG

(g/m

l)

1000

0

2000

3000

4000

5000

Antibody production comparingBD Cell Basal Medium with serumin a CELLine Device to RPMI with serum.

CELLine Membrane Technology

Cells are cultured in a 15 ml cultivation chamber separated from the 1000 ml nutrientsupply compartment by an upper semi-permeable membrane. Nutrients and other smallmolecules pass across the semi-permeable, cellulose acetate membrane of the cellcompartment. Any cell-secreted product with a molecular weight of over 10,000 Dalton is retained in the growth chamber of the device. The molded silicone membrane on thebottom of the device allows oxygen to reach the cells from underneath. The cells settleupon the bottom of the cell compartment atop of this gas exchange surface, across whichoxygen and carbon dioxide rapidly diffuse. This approach leads to high cell concentrationswithin the small cell compartment volume.

Separate ports provide selective access to the nutrient supply chamber and the cultivationchamber. The solid white cap identifies the cultivation chamber port while a blue vent capidentifies the nutrient supply chamber. The cell chamber is accessed via the cultivationchamber port via a serological pipet. The total system is transparent to allow visibility.

Metabolites

Nutrients

Oxygen

Cell products

Cell mass

Nutrientcompartment

Cellcompartment

Cultivationchamber port

Nutrientmembrane

Gasmembrane

O2 supply

Nutrient supplychamber

Cultivationchamber

Oxygen supplychamber

Transparent mountwith gas ducts


CultureSlides

BD Falcon™ CultureSlidesBD Falcon™ CultureSlides arecomprised of a glass slide prepared to provide a consistent surface for cell growth and a compartmentalizedchamber molded from medical-gradepolystyrene. BD Falcon CultureSlideshave an innovative sealing design that minimizes leakage. CultureSlidesallow researchers to culture cells and then analyze them on a glassmicroscope slide.

Cells are grown in a plastic chamberaffixed to a specially cleaned andtriple-rinsed glass slide.

Cells can be fixed and stained in placewithout disruption of the cell mono-layer. The chamber is easily and safely removed with an easy-to-usedisposable Safety Removal Tool.A simple safety removal tool lifts the plastic vessel off the glass slide. The adhesive gas-

ket remains with the vessel, not on the slide, facilitating further processing or place-ment of coverslips.

Primary bovine aortic endothelial cellsgrown on BD Falcon CultureSlides andstained with crystal violet. The bluehydrophobic grid defines the cell culturearea. A white writing patch provides clear sample identification. Well locationnumbers etched in the grid are clearlyvisible.

BD Falcon™ 1-well CultureSlidesGrowth Surface Total Volume Working Volume Qty./ Qty./Area per Well per Well per Well Pkg Case Cat. No.

8.6 cm2 10.0 ml 5.0 – 6.5 ml 12 96 35410112 24 354111


4.0 cm2 5.0 ml 2.0 – 2.5 ml 12 96 35410212 24 354112


1.7 cm2 2.5 ml 1.0 – 1.5 ml 12 96 35410412 24 354114


0.7 cm2 1.2 ml 0.7 – 0.8 ml 12 96 35410812 24 354118

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Cell Culture Tubes and Cell Scrapers

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BD Falcon CellCulture Tubes

BD Falcon™ Cell Culture TubesBD Falcon™ Cell Culture Tubes are TC-treated by vacuum-gas plasma andsterilized by gamma irradiation. Whitepolyethylene screw caps distinguishthe TC-treated tubes from general-purpose tubes and allow for both openand closed incubation. BD Falcon CellCulture Tubes are supplied in peel-open, medical-grade packaging. Cross-hatched index marking on the tubesassists researchers in positioning tubes consistently during repeatedhandling steps.

16 x 125 mm TubeQty./Bag Qty./Case Cat. No.

25 500 353033

Researchers can pick the cell scraperblade and handlelength that bestaccommodates their TC flask andindividual needs.

BD Falcon™ Cell ScrapersBD Falcon™ Cell Scrapers are avail-able in four convenient sizes and aresupplied individually in peel-open,medical-style packaging for sterilepresentation. The flexible, polystyrenehandle is designed with a specialtexture to ensure a firm grip. The BD Falcon Cell Scraper has beenthoughtfully designed to providemaximum accessibility to the growthsurfaces in a variety of culture vessels.The pivoting blade provides thecorrect angle for removal of cells from the entire growth surface.

18 cm Handle, 1.8 cm BladeRecommended for use with 25 cm2 and 12.5 cm2 flasks

Qty./Pack Qty./Case Cat. No.

1 100 353085

25 cm Handle, 1.8 cm Blade Recommended for use with 75 cm2 flasks


1 100 353086

25 cm Handle, 3.0 cm BladeRecommended for use with BD Falcon dishesand 75 cm2 flasks


1 100 353089

40 cm Handle, 3.0 cm BladeRecommended for use with 150 cm2 and 175 cm2 flasks and roller bottles


1 100 353087


Pre-tested BD Falcon™ In VitroFertilization (IVF) ProductsBD Falcon™ In Vitro Fertilization (IVF)products are the first plasticwareavailable that is certified sterile, non-pyrogenic and non-embryotoxic. Thesepre-tested products save you time andexpense in complying with the Collegeof American Pathologists (CAP) andAmerican Fertility Society (AFS) recommended standards for IVF labs.

Mouse embryo testing

Test articles are prepared in compli-ance with cGMP standards. Each lotof BD Falcon IVF product is tested forembryo toxicity using the 1-cell mouseembryo assay. Mouse embryos are iso-lated at the 1-cell stage from B6C3F1females following super ovulation withgonadotropin and mating with B6D2F1males. BD Falcon IVF products aretested using both a direct and indirecttest method. In the direct assay, theembryos are cultured directly in the

IVF labware. For the indirect assay,culture media is incubated in the IVFlabware for 24 hours at 37ºC. Theincubated media is then transferred toa culture plate and the 1-cell mouseembryo assay is performed. Productsare deemed non-embryotoxic if theysupport the growth of more than 75percent of the embryos to the expandedand/or hatched blastocyst stage.

To improve manipulation of ova andembryos, BD Biosciences designed, inconjunction with embryologists, aninnovative 4-well plate. A unique lidreduces the risk of contamination andminimizes evaporation by providingaccess to two wells at a time, whiletwo remain covered. The wells arenumbered and a large writing patchallows clear sample identification.Plates are packaged in individual peel-open trays for sterile presentation.

BD Falcon IVF Dishes and 4-well Platesare manufactured from virgin crys-talline polystyrene tested for USP Class

VI cytotoxicity. They have perfectly flat,optically clear surfaces for optimummanipulation and observation of ovaand embryos. Lids were designed foraseptic manipulation and consistentventing to maintain humidification.

60 mm Diameter DishWell area: 21.29 cm2; Well volume: 23.0 ml


20 500 353652

Center-well, 60 mm Diameter DishWell area: 2.89 cm2; Well volume: 2.5 ml; Total volume: 20 ml


20 500 353653

4-well PlateWell area: 1.39 cm2; Well volume: 1.8 ml

Qty./Tray Qty./Case Cat. No.

1 100 353654

Note: The lids of BD Falcon™ IVF Productsare not certified non-embryotoxic.

Innovative BD Falcon pre-tested andcertified plastic-ware for in vitrofertilization (IVF)

In Vitro Fertilization Plasticware

19

Product Class

bdbiosciences.com

BD BioCoat™ Collagen I Cultureware

354400 6-well plates 5 356400 6-well plates 50 354500 12-well plates 5 356500 12-well plates 50 354408 24-well plates 5 356408 24-well plates 50 354505 48-well plates 5 356505 48-well plates 50 354407 96-well clear plates 5 356407 96-well clear plates 50 356698 96-well clear plates 80 354649 96-well black/clear plates 5 356649 96-well black/clear plates 50 356700 96-well black/clear plates 80 354650 96-well white/clear plates 5 356650 96-well white/clear plates 50 356701 96-well white/clear plates 80 354519 96-well white plates 5 356519 96-well white plates 50 356699 96-well white plates 80 354666 384-well clear plates 5 356666 384-well clear plates 50356704 384-well clear plates 80 354667 384-well black/clear plates 5 356667 384-well black/clear plates 50 356705 384-well black/clear plates 80 354664 384-well white/clear plates 5 356664 384-well white/clear plates 50 356702 384-well white/clear plates 80 354665 384-well white plates 5 356665 384-well white plates 50 356703 384-well white plates 80 354456 35 mm culture dishes 20 356456 35 mm culture dishes 100 354401 60 mm culture dishes 20 356401 60 mm culture dishes 100 354450 100 mm culture dishes 10 356450 100 mm culture dishes 40 354551 150 mm culture dishes 5 354531 25 cm2 plug-seal flasks† 10 356531 25 cm2 plug-seal flasks† 50 354484 25 cm2 vented-cap flasks† 10 356484 25 cm2 vented-cap flasks† 50 354462 75 cm2 plug-seal flasks† 5 356462 75 cm2 plug-seal flasks† 50 354485 75 cm2 vented-cap flasks† 5 356485 75 cm2 vented-cap flasks† 50 354645 150 cm2 plug-seal flasks 5 356645 150 cm2 plug-seal flasks 40 354486 150 cm2 vented-cap flasks 5 356486 150 cm2 vented-cap flasks 40 354478 175 cm2 plug-seal flasks 5 356478 175 cm2 plug-seal flasks 40 354487 175 cm2 vented-cap flasks 5 356487 175 cm2 vented-cap flasks 40

354089 22 mm round No.1 60German glass coverslips

354556 1-well CultureSlides 12 354627 2-well CultureSlides 12 354557 4-well CultureSlides 12 354630 8-well CultureSlides 12

BD BioCoat™ Poly-D-Lysine Cultureware

354413 6-well plates 5 356413 6-well plates 50 354470 12-well plates 5 356470 12-well plates 50 354414 24-well plates 5 356414 24-well plates 50 354509 48-well plates 5 356509 48-well plates 50 354461 96-well clear plates 5 356461 96-well clear plates 50356690 96-well clear plates 80354640 96-well black/clear plates 5 356640 96-well black/clear plates 50 356692 96-well black/clear plates 80354651 96-well white/clear plates 5 356651 96-well white/clear plates 50 356693 96-well white/clear plates 80354620 96-well white plates 5 356620 96-well white plates 50 356691 96-well white plates 80354662 384-well clear plates 5 356662 384-well clear plates 50 356696 384-well clear plates 80 354663 384-well black/clear plates 5 356663 384-well black/clear plates 50 356697 384-well black/clear plates 80 354660 384-well white/clear plates 5 356660 384-well white/clear plates 50 356694 384-well white/clear plates 80 354661 384-well white plates 5 356661 384-well white plates 50 356695 384-well white plates 80 354467 35 mm culture dishes 20 356467 35 mm culture dishes 100 354468 60 mm culture dishes 20 356468 60 mm culture dishes 100 354469 100 mm culture dishes 10 356469 100 mm culture dishes 40 354550 150 mm culture dishes 5 354479 25 cm2 plug-seal flasks† 10 356479 25 cm2 plug-seal flasks† 50 354536 25 cm2 vented-cap flasks† 10 356536 25 cm2 vented-cap flasks† 50 354524 75 cm2 plug-seal flasks† 5 356524 75 cm2 plug-seal flasks† 50 354537 75 cm2 vented-cap flasks† 5 356537 75 cm2 vented-cap flasks† 50 354495 150 cm2 plug-seal flasks 5 356495 150 cm2 plug-seal flasks 40

354538 150 cm2 vented-cap flasks 5 356538 150 cm2 vented-cap flasks 40 354529 175 cm2 plug-seal flasks 5 356529 175 cm2 plug-seal flasks 40 354539 175 cm2 vented-cap flasks 5 356539 175 cm2 vented-cap flasks 40 354077 35 mm coverslip-bottom dishes 20354086 12 mm round No.1 80

German glass coverslips 354566 1-well CultureSlides 12 354629 2-well CultureSlides 12 354577 4-well CultureSlides 12 354632 8-well CultureSlides 12

BD BioCoat™ Poly-L-Lysine Cultureware

354515 6-well plates 5 356515 6-well plates 50 354516 96-well clear plates 5 356516 96-well clear plates 50 354518 35 mm culture dishes 20 356518 35 mm culture dishes 100 354517 60 mm culture dishes 20 356517 60 mm culture dishes 100 354085 12 mm round No.1 80

German glass coverslips

BD BioCoat™ Gelatin Cultureware

354652 6-well plates 5 356652 6-well plates 50 354689 96-well plates 5356689 96-well plates 50354653 100 mm culture dishes 10 356653 100 mm culture dishes 40 354654 75 cm2 plug-seal flasks† 5 356654 75 cm2 plug-seal flasks† 50 354488 75 cm2 vented-cap flasks† 5 356488 75 cm2 vented-cap flasks† 50

BD BioCoat™ Collagen IV Cultureware

354428 6-well plates 5 354430 24-well plates 5 354429 96-well plates 5 354459 35 mm culture dishes 20 354416 60 mm culture dishes 20 354453 100 mm culture dishes 10 354554 150 mm culture dishes 5 354534 25 cm2 plug-seal flasks† 10 354523 75 cm2 plug-seal flasks† 10 354528 175 cm2 plug-seal flasks 5

BD BioCoat™ EHS Natrix™ Cultureware

354418 6-well plates 5

Cat. No. Description Qty./Case Cat. No. Description Qty./Case Cat. No. Description Qty./Case

BD BioCoat™ Cultureware

BD BioCoat™ Cultureware is a unique line of tissue culture vessels, including plates, dishes, flasks, cultureslides, and coverslips,coated with a variety of extracellular matrix (ECM) proteins and attachment factors. The various ECM components are appliedto vessel surfaces by a proprietary manufacturing process resulting in uniform, optically clear matrix substrates. This technology,together with our exacting quality control, guarantees the performance of each lot, as well as consistency from lot to lot.

†BD BioCoat 25 cm2 flasks are 70 ml canted neck; BD BioCoat 75 cm2 flasks are 250 ml canted neck.

BD BioCoat™ Laminin Cultureware

354404 6-well plates 5 354502 12-well plates 5 354412 24-well plates 5 354507 48-well plates 5 354410 96-well plates 5 354458 35 mm culture dishes 20 354405 60 mm culture dishes 20 354452 100 mm culture dishes 10 354553 150 mm culture dishes 5 354533 25 cm2 plug-seal flasks† 10 354522 75 cm2 plug-seal flasks† 10

BD BioCoat™ Fibronectin Cultureware

354402 6-well plates 5 354501 12-well plates 5 354411 24-well plates 5 354506 48-well plates 5 354409 96-well plates 5 354457 35 mm culture dishes 20 354403 60 mm culture dishes 20 354451 100 mm culture dishes 10 354552 150 mm culture dishes 5 354532 25 cm2 plug-seal flasks† 10 354521 75 cm2 plug-seal flasks† 10 354646 150 cm2 plug-seal flasks 5 354526 175 cm2 plug-seal flasks 5 354088 22 mm round No.1 60

German glass coverslips354558 1-well CultureSlides 12 354628 2-well CultureSlides 12 354559 4-well CultureSlides 12 354631 8-well CultureSlides 12

BD BioCoat™ Laminin/Fibronectin Cultureware


BD BioCoat ™Matrigel™ Matrix Cultureware

354432 6-well plates 2 354503 12-well plates 2 354433 24-well plates 2 354508 48-well plates 2 354460 35 mm culture dishes 8

BD BioCoat™ Matrigel Matrix Cultureware –Thin Layer

354603 6-well plates 5 354605 24-well plates 5 354607 96-well plates 5 354602 35 mm culture dishes 20 354601 60 mm culture dishes 20 354600 100 mm culture dishes 10

BD BioCoat™ Matrigel Matrix Culturewarefor Hepatocytes

354510 6-well plates 5 354634 100 mm culture dishes 5

BD BioCoat™ Growth Factor Reduced Matrigel Matrix for Smooth Muscle Cells


BD BioCoat™ Poly-D-Lysine/LamininCultureware

354595 6-well plates 5 354619 24-well plates 5 354596 96-well plates 5 354455 100 mm culture dishes 10 354087 12 mm round No.1 80

German glass coverslips 354687 2-well Cultureslides 12354688 8-well Cultureslides 12

BD BioCoat™ Poly-L-Ornithine/LamininCultureware

354658 6-well plates 5 354659 24-well plates 5 354657 96-well plates 5

Vented Caps for BD BioCoat™ Flasks

354637 25 cm2 vented caps 100 354638 75 cm2 vented caps 100 354639 175 cm2 vented caps 50

BD BioCoat™ Variety Pack Cultureware

354417 6-well plates 5 includes: Collagen I, Fibronectin,Laminin, Poly-D-Lysine, and BD Falcon™ Plates

354431 6-well plates 5 includes: Collagen I, Collagen IV, Fibronectin, Laminin, and Poly-D-Lysine Plates

354655 2-well CultureSlides 12 includes: Collagen I, Fibronectin, Poly-D-Lysine, and BD Falcon™

CultureSlides354656 8-well CultureSlides 12

includes: Collagen I, Fibronectin, Poly-D-Lysine, and BD Falcon™

CultureSlides

Cat. No. Description Qty./Case Cat. No. Description Qty./Case Cat. No. Description Qty./Case

BD BioCoat™ Coated Cultureware

†BD BioCoat 25 cm2 flasks are 70 ml canted neck; BD BioCoat 75 cm2 flasks are 250 ml canted neck.

CELLine is a trademark of Integra Biosciences AG.SelecT is a trademark of The Automation Partnership, Royston, Herts, UK.BD, BD Logo, and all other trademarks are the property of Becton, Dickinson and Company. ©2006 BDB00F022 Rev. 2

To order BD Falcon™ products, contact your authorized distributor.To order BD BioCoat™ Cultureware, BD Cell™ MAb Medium, and CELLine™ Flask, contact our CustomerService Department at: tel: 877.232.8995; fax: 800.325.9637 or 858.812.8889

For technical assistance on BD Falcon or BD BioCoat products, contact our Technical Services Department at:tel: 877.232.8995 or 978.901.7389; fax: 978.901.7491; e-mail: [email protected]

For technical information on BD Cell MAb Medium, contact our Application Specialist at:tel: 800.219.7174 x1933 or 760.788.4577

BD BiosciencesTwo Oak ParkBedford, MA 01730 USAtel: 877.232.8995fax: 800.325.9637

BD2280 Argentia RoadMississauga, OntarioCanada L5N 6H8tel: 866.979.9408fax: 800.565.0897

Nippon BDAkasaka DS Bldg.5-26 Akasaka 8-chomeMinato-ku, Tokyo 107 Japantel: (81) 24 593 5405fax: (81) 24 593 5761

BD BiosciencesSingapore Branch30 Tuas Avenue 2Singapore 639461tel: (65) 6861 0633fax: (65) 6860 1590

BD Biosciences EuropeErembodegem-Dorp 869320 Erembodegem, Belgiumtel: (32) 53 720 211fax: (32) 53 720 450e-mail: [email protected]

BD Biosciences4 Research Park DriveMacquarie University Research ParkNorth Ryde NSW 2113 Australiatel: (612) 8875 5239fax: (612) 8875 7200

cell culture brochure - bd

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