School of Chemical Sciences Seminar

Novel laser sources for flow cytometry: Covering the entire visible spectrum

Dr Bill TELFORD

National Cancer Institute, National Institutes of Health (Bethesda, MD, USA)

Tuesday 17th July at 2:00 PM in Room 301-242The lecture will be followed by a sherry hour in the SCS tea room on 5th floor at 3 PM

Abstract

In the last ten years we have seen a dramatic jump in the data collection and analysis capabilities of flow cytometers and other biomedical technologies usin fluorescence. Multi-laser instruments are now the norm, with at least three lasers often being the minimum for comprehensive analysis. Simultaneous analysis of eight or more fluorescent parameters is now routine. The variety of fluorescent probes available for multicolor labeling has greatly expanded, and the software allowing analysis of high-dimensional cytometry data is increasing in capability and complexity. Imaging has become a component of our cytometric analysis, opening up whole new avenues for individual cell analysis. So where do we go from here? . Laser technology is now available that makes our excitation capabilities essentially limitless. We will discuss developments in laser technology that should continue to increase both the number of parameters we can analyze, and expand the flexibility of our instruments to excite and detect the ever-expanding palette of fluorescent probes available to life scientists.

Bio

Dr. Telford is a head of the Flow Cytometry Core Laboratory at the Experimental Transplantation and Immunology Branch, National Cancer Institute, National Institutes of Health (Bethesda, MD, USA).