cd25 and indoleamine 2,3-dioxygenase are up-regulated by prostaglandin e2 and expressed by...
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![Page 1: CD25 and indoleamine 2,3-dioxygenase are up-regulated by prostaglandin E2 and expressed by tumor-associated dendritic cells in vivo: additional mechanisms](https://reader035.vdocuments.us/reader035/viewer/2022062422/56649ef55503460f94c08650/html5/thumbnails/1.jpg)
CD25 and indoleamine 2,3-dioxygenase are up-regulated by prostaglandin E2 and expressed by tumor-associated dendritic cells in vivo: additional mechanisms of T-cell
inhibition
by Michael S. von Bergwelt-Baildon, Alexey Popov, Tomo Saric, Jens Chemnitz, Sabine Classen, Marc S. Stoffel, Francesca Fiore, Udo Roth, Marc Beyer, Svenja Debey, Claudia Wickenhauser, Franz-Georg Hanisch, and Joachim L. Schultze
BloodVolume 108(1):228-237
July 1, 2006
©2006 by American Society of Hematology
![Page 2: CD25 and indoleamine 2,3-dioxygenase are up-regulated by prostaglandin E2 and expressed by tumor-associated dendritic cells in vivo: additional mechanisms](https://reader035.vdocuments.us/reader035/viewer/2022062422/56649ef55503460f94c08650/html5/thumbnails/2.jpg)
Addition of PGE2 to maturing DCs changes their morphology and IL12 production.
Michael S. von Bergwelt-Baildon et al. Blood 2006;108:228-237
©2006 by American Society of Hematology
![Page 3: CD25 and indoleamine 2,3-dioxygenase are up-regulated by prostaglandin E2 and expressed by tumor-associated dendritic cells in vivo: additional mechanisms](https://reader035.vdocuments.us/reader035/viewer/2022062422/56649ef55503460f94c08650/html5/thumbnails/3.jpg)
Induction of CD25 in monocyte-derived DCs. (A) Analysis of cell-surface expression of CD25 by the different DC populations using flow cytometry.
Michael S. von Bergwelt-Baildon et al. Blood 2006;108:228-237
©2006 by American Society of Hematology
![Page 4: CD25 and indoleamine 2,3-dioxygenase are up-regulated by prostaglandin E2 and expressed by tumor-associated dendritic cells in vivo: additional mechanisms](https://reader035.vdocuments.us/reader035/viewer/2022062422/56649ef55503460f94c08650/html5/thumbnails/4.jpg)
Induction of CD25 in BDCA-1+ myeloid DCs. (A) Flow cytometry was performed after 18 hours of culture to assess cell-surface expression of CD1c, CD83, CD25 (x-axis), and HLA-DR (y-axis).
Michael S. von Bergwelt-Baildon et al. Blood 2006;108:228-237
©2006 by American Society of Hematology
![Page 5: CD25 and indoleamine 2,3-dioxygenase are up-regulated by prostaglandin E2 and expressed by tumor-associated dendritic cells in vivo: additional mechanisms](https://reader035.vdocuments.us/reader035/viewer/2022062422/56649ef55503460f94c08650/html5/thumbnails/5.jpg)
Expression and function of IDO in monocyte-derived DCs and myeloid BDCA-1+ DCs. mRNA for RT-PCR and cell lysates for immunoblot analysis were obtained from monocytes, imDCs, or
maDCs cultured in the presence or absence of PGE2.
Michael S. von Bergwelt-Baildon et al. Blood 2006;108:228-237
©2006 by American Society of Hematology
![Page 6: CD25 and indoleamine 2,3-dioxygenase are up-regulated by prostaglandin E2 and expressed by tumor-associated dendritic cells in vivo: additional mechanisms](https://reader035.vdocuments.us/reader035/viewer/2022062422/56649ef55503460f94c08650/html5/thumbnails/6.jpg)
Monocyte-derived DCs retain the expression of functional IDO and CD25 after removal of PGE2 and maturation signals.
Michael S. von Bergwelt-Baildon et al. Blood 2006;108:228-237
©2006 by American Society of Hematology
![Page 7: CD25 and indoleamine 2,3-dioxygenase are up-regulated by prostaglandin E2 and expressed by tumor-associated dendritic cells in vivo: additional mechanisms](https://reader035.vdocuments.us/reader035/viewer/2022062422/56649ef55503460f94c08650/html5/thumbnails/7.jpg)
Influence of DC supernatants on T-cell proliferation.
Michael S. von Bergwelt-Baildon et al. Blood 2006;108:228-237
©2006 by American Society of Hematology
![Page 8: CD25 and indoleamine 2,3-dioxygenase are up-regulated by prostaglandin E2 and expressed by tumor-associated dendritic cells in vivo: additional mechanisms](https://reader035.vdocuments.us/reader035/viewer/2022062422/56649ef55503460f94c08650/html5/thumbnails/8.jpg)
Expression of CD25 and IDO in peritumoral dendritic cells.
Michael S. von Bergwelt-Baildon et al. Blood 2006;108:228-237
©2006 by American Society of Hematology