Supplementary Data:

Table S1. Sample codes, geographic coordinates of sampling sites, control region haplotypes and morphological

classification of the 67 analysed hedgehogs. Dashes indicate samples for which we did not obtain control region

sequences.

1

Sample number Sample code Geographic latitude and longitude Haplotype Morphogroup

2

1 Adrar1 26°43'40.27"N 0°10'12.54"E H1 P. aethiopicus

2 Adrar2 28°35'47.71"N 0°2'24.80"E H1 P. aethiopicus

3 Adrar3 27°45'36.11"N 0°15'47.47" W H2 A. algirus I

4 Adrar4 28°23'12.68"N 0°14'0.31" W H3 A. algirus I

5 Algiers1 36°39'46.64"N 2°56'58.04"E H2 A. algirus I

6 Algiers2 36°36'45.06"N 3°6'51.83"E H3 A. algirus II

7 Algiers3 36°44'36.57"N 3°0'57.76"E - A. algirus II

8 Algiers4 36°43'8.72"N 3°19'35.10"E - A. algirus I

9 Algiers5 36°42'17.31"N 2°54'29.99"E H4 A. algirus I

10 Algiers6 36°47'27.34"N 3°17'14.65"E - A. algirus II

11 Algiers7 36°39'13.40"N 3°8'29.41"E - A. algirus I

12 Algiers8 36°41'57.57"N 3°13'12.87"E - A. algirus I

13 Batna1 35°38'27.76"N 5°58'55.52"E H5 A. algirus I

14 Batna2 35°31'27.95"N 6°0'3.41"E H5 A. algirus I

15 Batna3 35°37'13.46"N 6°15'3.10"E H6 A. algirus I

16 Béchar1 30° 7'21.07"N 2°10'16.89" W H7 P. aethiopicus

17 Béchar2 30°7'9.95"N 2°10'19.79" W H8 P. aethiopicus

18 Béchar3 30°6'29.66"N 2°10'8.91" W H9 P. aethiopicus

19 Béchar4 30°20'9.46"N 2°17'19.97" W H10 P. aethiopicus

20 Béchar5 29°54'31.67"N 2°17'8.54" W H11 P. aethiopicus

21 Béchar6 30°2'3.85"N 1°52'51.60" W H11 P. aethiopicus

22 Béchar7 31°36'34.59"N 2°14'50.99" W H12 P. aethiopicus

23 Béchar8 30° 7'41.61"N 2°10'23.29" W - P. aethiopicus

24 Biskara 34°42'16.31"N 5°24'4.32"E H13 A. algirus I

25 Blida1 36°33'18.92"N 3°7'56.06"E H2 A. algirus II

26 Blida2 36°31'36.36"N 3°4'24.87"E H13 A. algirus II

27 Blida3 36°36'36.60"N 3°13'42.03"E H4 A. algirus II

28 Blida4 36°32'3.94"N 2°59'45.31"E H2 A. algirus II

29 Blida5 36°34'20.41"N 3°9'49.43"E H4 A. algirus I

30 Blida6 36°33'33.17"N 3°9'10.07"E H4 A. algirus II

31 Blida7 36°25'29.66"N 2°52'33.33"E H14 A. algirus I

32 Blida8 36°34'51.57"N 3°15'51.47"E H4 A. algirus I

33 B-Bou Arreridj 35°55'58.80"N 5°2'11.49"E H15 A. algirus I

34 Bouira 36°31'47.81"N 3°41'18.59"E H13 A. algirus I

35 Boumerdès1 36°44'39.93"N 3°42'48.93"E H13 A. algirus I

36 Boumerdès2 36°49'17.36"N 3°51'48.89"E H3 A. algirus II

37 Boumerdès3 36°45'40.23"N 3°27'49.22"E H16 A. algirus II

38 Boumerdès4 36°44'47.63"N 3°43'30.69"E H13 A. algirus I

39 Boumerdès5 36°44'24.14"N 3°43'14.92"E H17 A. algirus I

3

40 Constantine1 36°22'13.10"N 6°28'36.89"E - A. algirus I

41 Constantine2 36°21'26.94"N 6°27'36.19"E - A. algirus II

42 Djelfa1 35°26'47.17"N 2°54'26.87"E H13 A. algirus I

43 Djelfa2 34°39'36.19"N 3°15'20.81"E H4 A. algirus I

44 El-Oued1 33°57'90.59"N 5°56'32.31"E H18 A. algirus II

45 El-Oued2 33°22'6.05"N 6°52'22.23"E H19 A. algirus I

46 El-Oued3 33°18'58.05"N 6°53'54.43"E H19 A. algirus I

47 Ghardaïa1 30°35'46.04"N 2°53'0.66"E H7 P. aethiopicus

48 Ghardaïa2 30°32'50.29"N 2°54'57.51"E H20 P. aethiopicus

49 Ghardaïa3 30°34'6.55"N 2°52'43.59"E H21 P. aethiopicus

50 Khenchela 35°26'15.23"N 7°9'18.87"E H22 A. algirus I

51 Laghouat 32°55'42.64"N 3°15'49.54"E H3 A. algirus I

52 Médéa1 36°15'24.02"N 2°44'21.15"E H23 A. algirus II

53 Médéa2 36°12'58.02"N 3°6'26.89"E H3 A. algirus I

54 Médéa3 36°21'34.84"N 2°46'1.35"E - A. algirus I

55 Mila 36°23'45.82"N 6°24'17.50"E - A. algirus I

56 Msila 35°12'40.09"N 4°11'6.10"E - A. algirus I

57 Ouargla1 33° 3'39.36"N 6° 3'4.46"E H24 P. aethiopicus

58 Ouargla2 33°4'41.66"N 6° 3'33.14"E H5 A. algirus I

59 Sétif1 36°19'3.66"N 4°50'26.84"E H25 A. algirus II

60 Sétif2 36°18'52.45"N 4°50'22.22"E H25 A. algirus II

61 Tamanrasset 27°11'3.80"N 2°28'32.11"E H26 P. aethiopicus

62 Tipaza1 36°34'59.08"N 2°7'0.94"E H2 A. algirus II

63 Tipaza2 36°42'26.29"N 2°51'20.56"E H13 A. algirus I

64 Tipaza3 36°40'52.46"N 2°52'39.38"E H2 A. algirus I

65 Tlemcen1 34°52'8.50"N 1°20'40.91" W H13 A. algirus II

66 Tlemcen2 34°52'27.73"N 1°21'5.27" W H13 A. algirus II

67 Tlemcen3 34°51'53.28"N 1°22'10.23" W H13 A. algirus I

4

Table S2. Quantitative and qualitative variables used in the morphological analysis.

Quantitative variables were body weight (W, in gr), head and body length (Lb, in mm), hind foot length (Lpp, in

mm), tail length (Lt, in mm) and spine length (Lp, in mm). Qualitative variables included the coloration of the

spines (black and white, Cpblw; brown and beige, Cpbrb) and of the body (black and white, Cbblw; beige, Cbb;

brown, Cbbr; black and white with patches, Cbblws), and the size (small, Ses; large, Sel) and shape (round, Sher;

pointy, Shep) of the ears.

Individual

Quantitative variables Qualitative variables

Lb WLpp Lt Lp

Cpblw

Cpbrb

Cbblw

Cbb

Cbbr

Cbblws Ses Sel

Sher

Shep

Adrar1175

270 20 20 15 0 1 0 0 0 1 0 1 0 1

Adrar2180

280 21 22 16 1 0 0 0 0 1 0 1 0 1

Adrar3250

510 34 29 20 1 0 1 0 0 0 1 0 0 1

Adrar4170

265 20 20 15 0 1 1 0 0 0 1 0 0 1

Algiers1172

265 19 19 15 1 0 1 0 0 0 1 0 0 1

Algiers2250

514 35 29 20 0 1 0 0 1 0 1 0 1 0

Algiers3264

540 37 38 22 0 1 0 0 1 0 1 0 1 0

Algiers4210

400 26 23 18 0 1 1 0 0 0 1 0 0 1

Algiers5172

265 21 19 15 1 0 1 0 0 0 1 0 0 1

Algiers6262

540 36 38 21 0 1 0 0 1 0 1 0 1 0

Algiers7173

275 22 20 17 1 0 1 0 0 0 1 0 0 1

Algiers8182

284 25 23 18 1 0 1 0 0 0 1 0 0 1

Batna1219

410 28 24 18 0 1 1 0 0 0 1 0 0 1

Batna2235

440 32 25 19 1 0 1 0 0 0 1 0 0 1

Batna3180

282 24 23 18 1 0 1 0 0 0 1 0 0 1

Béchar1235

260 34 31 19 1 0 0 0 0 1 0 1 0 1

Béchar2230

256 34 30 19 0 1 0 0 0 1 0 1 0 1

Béchar3233

500 34 27 19 1 0 0 0 0 1 0 1 0 1

5

Béchar4235

499 34 31 19 1 0 0 0 0 1 0 1 0 1

Béchar5175

280 22 19 19 1 0 0 0 0 1 0 1 0 1

Béchar6180

305 23 21 21 1 0 0 0 0 1 0 1 0 1

Béchar7235

480 34 25 19 1 0 0 0 0 1 0 1 0 1

Béchar8236

481 34 25 19 1 0 0 0 0 1 0 1 0 1

Biskara175

270 21 20 16 0 1 1 0 0 0 1 0 0 1

Blida1175

270 22 20 16 0 1 0 1 0 0 1 0 1 0

Blida2245

505 33 27 19 0 1 0 0 1 0 1 0 1 0

Blida3245

500 33 27 20 0 1 0 0 1 0 1 0 1 0

Blida4265

557 37 38 22 0 1 0 0 1 0 1 0 1 0

Blida5179

280 22 21 17 0 1 1 0 0 0 1 0 0 1

Blida6245

480 33 27 19 0 1 0 0 1 0 1 0 1 0

Blida7180

285 24 22 18 0 1 1 0 0 0 1 0 0 1

Blida8246

510 33 28 19 1 0 1 0 0 0 1 0 0 1

BouArreridj220

415 31 24 19 1 0 1 0 0 0 1 0 0 1

Bouira200

385 26 21 18 1 0 1 0 0 0 1 0 0 1

Boumerdès1230

420 32 25 19 1 0 1 0 0 0 1 0 0 1

Boumerdès2247

510 33 28 20 0 1 0 0 1 0 1 0 1 0

Boumerdès3248

513 34 28 20 0 1 0 0 1 0 1 0 1 0

Boumerdès4250

515 35 30 20 1 0 1 0 0 0 1 0 0 1

Boumerdès5245

500 30 25 19 1 0 1 0 0 0 1 0 0 1

Constantine1

190

290 27 25 18 1 0 1 0 0 0 1 0 0 1

Constantine2

256

530 36 31 20 0 1 0 1 0 0 1 0 1 0

Djelfa1250

515 20 30 20 1 0 1 0 0 0 1 0 0 1

Djelfa2210

405 27 23 18 1 0 1 0 0 0 1 0 0 1

El-Oued1190

379 25 21 17 0 1 0 1 0 0 1 0 1 0

El-Oued2220

410 29 24 18 1 0 1 0 0 0 1 0 0 1

El-Oued3178

278 23 22 17 1 0 1 0 0 0 1 0 0 1

Ghardaïa1235

498 34 31 19 0 1 0 0 0 1 0 1 0 1

Ghardaïa2233

490 34 31 18 0 1 0 0 0 1 0 1 0 1

6

Ghardaïa3230

486 32 30 19 0 1 0 0 0 1 0 1 0 1

Khenchela205

390 27 22 19 1 0 1 0 0 0 1 0 0 1

Laghouat174

271 22 20 17 0 1 1 0 0 0 1 0 0 1

Médéa1210

400 26 22 18 0 1 0 1 0 0 1 0 1 0

Médéa2244

500 31 25 19 1 0 1 0 0 0 1 0 0 1

Médéa3240

490 30 24 18 0 1 1 0 0 0 1 0 0 1

Mila191

292 27 25 18 0 1 1 0 0 0 1 0 0 1

Msila233

437 33 25 19 1 0 1 0 0 0 1 0 0 1

Ouargla1230

422 32 25 19 0 1 0 0 0 1 0 1 0 1

Ouargla2189

315 25 22 24 1 0 1 0 0 0 1 0 0 1

Sétif1255

525 36 31 20 0 1 0 1 0 0 1 0 1 0

Sétif2250

520 34 30 20 0 1 0 1 0 0 1 0 1 0

Tamanrasset190

350 25 22 19 0 1 0 0 0 1 0 1 0 1

Tipaza1270

560 38 40 23 0 1 0 0 1 0 1 0 1 0

Tipaza2230

435 31 24 18 1 0 1 0 0 0 1 0 0 1

Tipaza3173

267 20 19 16 1 0 1 0 0 0 1 0 0 1

Tlemcen1270

558 38 40 23 0 1 0 0 1 0 1 0 1 0

Tlemcen2250

490 33 27 19 0 1 0 0 1 0 1 0 1 0

Tlemcen3200

380 26 21 17 0 1 1 0 0 0 1 0 0 1

7

Fig. S1. Two-dimensional scatter plot depicting morphological relationships among hedgehog individuals as

estimated by nonmetric multidimensional scaling (NMDS) ordination on a matrix of pairwise Gower distances.

Stress value was equal to 0.19. The distribution of the discrete morphological groups is represented by bivariate

95% concentration ellipses.

8

9

Fig. S2. Condensed UPGMA tree based on Gower distances between hedgehog individuals for the

morphological characters analysed. Numbers above branches indicate the percentage of bootstraps (cut-off value

of 50%) based on 1000 replicates. The three main clusters in the tree correspond to Paraechinus aethiopicus and

to the two morphotypes identified within Atelerix algirus.

A B

C D

FE

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Fig. S3. Representative pictures of the two morphotypes identified within Atelerix algirus, ‘algirus I’ (images A

and B, different specimens) and ‘algirus II’ (images C and D, different specimens), and of Paraechinus

aethiopicus (images E and F, different specimens).

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