cd 3200 service manual
TRANSCRIPT
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1 - Introduction
2 Troubleshooting
3 -Parts Lists
4 -Removal & Replacement
5 -Verification Procedures
CELL-DYN3200
SERVICE
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300August 1998 1 - 1
4 0 ,QWURGXFWLRQ Proprietary Information
4 0 ,QWURGXFWLRQ
Table of Contents
1.1 Proprietary Information . . . . . . . . . . . . . . . . . . . . . . . . . 1
1.2 Revision Log. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
1.3 About This Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Service Manual Section Descriptions . . . . . . . . . . . . . 3
Section 1: Introduction & General Data . . . . . . . . . . . . 3
Section 2: Troubleshooting . . . . . . . . . . . . . . . . . . . . . . 3
Section 3: Parts Lists . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Section 4: Removal & Replacement . . . . . . . . . . . . . . . 3
Section 5: Verification Procedures . . . . . . . . . . . . . . . . 3
Section 6: Preventive Maintenance & Total Call. . . . . . 3
1.4 CELL-DYN 3200 Operators Manual . . . . . . . . . . . . . . 3
1.5 Warranty Information . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
1.6 System Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
The CELL-DYN 3200 Hematology System . . . . . . . . . . 4
System External Components . . . . . . . . . . . . . . . . . . . 4
System Internal Components . . . . . . . . . . . . . . . . . . . . 5
1.7 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Signal Words and Graphic Warning Conventions . . . 5Signal Words . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Graphic Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Biosafety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Electrical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Laser Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.8 CELL-DYN 3200 Hematology Parameters. . . . . . . . . . 9
1.9 System Specifications . . . . . . . . . . . . . . . . . . . . . . . . . 10
1.1 Proprietary InformationThis CELL- DYN 3200 System Service Manual was developedsolely for use with Abbott Laboratories equipment as specified
in the operating instructions. The Manual is copyrighted byAbbott Laboratories. No part of this document may bereproduced, stored, or transmitted in any form or by any meanselectronic, mechanical, photocopied, recorded, or otherwisewithout the prior written permission of Abbott Laboratories.
This CELL- DYN 3200 System Service Manual is published byAbbott Diagnostics, a Division of Abbott Laboratories, AbbottPark, IL 60064-3500, U. S. A. Please direct all inquiriesconcerning information in this manual to the foregoing address.
CELL- DYNis a registered trademark of Abbott Laboratories.
Vacutaineris a registered trademark of Becton-Dickinson andCompany.
This service manual was developed to be used by trainedAbbott Laboratories Field Service Personnel. The revisionstatus of this manual is the responsibility of the manual holder.
In no event shall Abbott Laboratories or its subsidiaries be
liable for any damages incurred in connection with or arisingfrom the use of this manual by persons not fully trained byAbbott Laboratories.
1998 by Abbott Laboratories. All rights reserved.
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300August 1998 1 - 2
4 0 ,QWURGXFWLRQ 1.2 Revision Log
1.2 Revision Log
The Revision Status of this manual is indicated below. Be surethat the manual contains the latest revision number of allpages.
Table 1-1 Revision Log
Revision Date Pages Revised/Added TSBs Incorporated ISAs Incorporated
A August Complete Revision 109 - 001A, 003 109 - 003, 004, 005, 006
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4 0 ,QWURGXFWLRQ 1.3 About This Manual
1.3 About This Manual
Introduction
This Service Manual contains the information necessary toalign, troubleshoot, repair, and maintain the CELL-DYN 3200
Automated Hematology System.
Service Manual Section Descriptions
The manual is composed of five sections. The following aredescriptions of the contents of each of the sections.
Section 1: Introduction
This section contains an introduction to the system, information
on the use of the manual, warranty information, safetyconsiderations, hematology parameters, and systemspecifications.
Section 2: Troubleshooting
This section contains procedures to be used by the FSR inidentifying and isolating problems. The procedures arepresented in a flowchart format and are accompanied byillustrations for added clarity. A detailed explanation of the use
of the flowcharts is given at the beginning of the section.
Section 3: Parts Lists
This section contains parts lists and graphics showing thelocations of FRUs. This section is used by the FSR inconjunction with Troubleshooting and Removal & Replacement.
Section 4: Removal & Replacement
This section contains the procedures to be used by the FSR forthe removal and replacement of subassemblies andcomponents. The procedures are presented in a paragraphformat and are accompanied by illustrations for added clarity.
Section 5: Verification Procedures
This section contains procedures that enable the FSR to verifythe integrity of various instrument functions. These proceduresmay be used to facilitate troubleshooting, or be dictated byTroubleshooting and Removal and Replacement.
This section also contains procedures such as adjustments,alignments, and calibrations used to ensure proper instrumentperformance. These procedures may be performed on a routine
maintenance basis, or be dictated by Removal andReplacement.
1.4 CELL-DYN 3200 Operators ManualOperating instructions and routine maintenance procedures forthe CELL-DYN 3200 are included in the Operators Manualprovided with each system. It is essential that the serviceengineer read and understand the Operators Manual, and beable to correctly perform all routine operating functions before
attempting to troubleshoot and repair the system.
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4 0 ,QWURGXFWLRQ 1.5 Warranty Information
1.5 Warranty InformationComponents comprising the CELL-DYN 3200 System havebeen designed by Abbott Laboratories for optimal performance.Substitution of reagents, accessories, or system components
may adversely affect performance and may invalidate anywarranty agreements.
Abbott Laboratories cannot accept responsibility for any clinicalresults produced by use of reagents, calibrators, controls,
equipment, or supplies either not manufactured by or notapproved by Abbott Laboratories.
1.6 System IntroductionEach CELL-DYN 3200 System may be comprised of three orfour major hardware components: Analyzer, Video Display,Keyboard, and optional Sample Loader.
The CELL-DYN 3200 Hematology System
When approaching a hematology system from a serviceperspective, the word system must be emphasized becausethere are both internal components (system hardware andsoftware) and external components (reagents, controls, etc.)that comprise the complete system. The Service
Representative must realize that both of these types ofcomponents can adversely affect overall system performance.Therefore, it follows that all external components andconditions such as reagents, environment, integrity of samplesand controls be checked and verified as correct beforeperforming service on the internal components. In theinvestigation of any non-specific or data related complaint, the
system hardware and software should be the last componentsof the system to be suspected as the cause of the problem.
System External Components
The following is a list of the CELL-DYN 3200 externalcomponents and conditions:
Operator Technique (Maintenance)
System Reagents
Isotonic Diluent
Diluent/Sheath
Hemoglobin Lyse
WBC Lyse
System Controls & Calibrators
CELL-DYN 3200 Whole Blood Control
OPTI-CAL
Latex Beads
Whole Blood Specimens
System Environmental Integrity (Temperature, RFI, ESD)
Power Line Integrity
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4 0 ,QWURGXFWLRQ 1.7 Safety
System Internal Components
The following is a list of the CELL-DYN 3200 internalcomponents:
CELL-DYN 3200 System Components
Flow Panel
Optics Bench
Power Supplies
Measurement Electronics
Computer Electronics
A/D & D/A Electronics
Air Supply
Personal Computer Module
Tube Manipulation Hardware
1.7 SafetyOperation, maintenance, and servicing of hematology systemsmay expose individuals to potential safety and health hazards.All work must be performed in accordance with proceduresdescribed in the Abbott Operators and Service Manuals, or asdirected by an Abbott Technical Support Representative.
Warnings are inserted in this manual to alert personnel to
potential hazards. The standard warning conventions includingsignal words (e.g. Caution) and symbols are described below.
Signal Words and Graphic Warning
Conventions
Signal Words
'$1*(5= Denotes an immediate hazard which, if not
avoided, could result in serious injury or death. This signal wordrepresents the highest level of any hazardous situation.
:$51,1*= Denotes a hazard which, if not avoided, couldresult in moderate to serious injury.
&$87,21= Denotes potential hazards that could result inminor injury. Also, used for conditions or activities that could
threaten equipment performance.
Graphic Conventions
The general hazard icon identifies an activity or areathat may present a hazard to personnel or equipment.
The electrical hazard icon alerts personnel to thepossibility of electrical shock if procedural or
engineering controls are not observed.
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300August 1998 1 - 6
4 0 ,QWURGXFWLRQ 1.7 Safety
Biosafety
Clean-up spills of potentially infectious materials in accordancewith established biosafety practices. A generally acceptedprocedure for cleaning such spills is to absorb the spill withtoweling or other absorbent material, wipe the area with a
detergent solution, and then wipe the area with an appropriatetuberculocidal disinfectant such as 10% chlorine bleachsolution.
Prior to service or maintenance, the instrument should be
decontaminated in accordance with the following:
a. Remove and dispose of contaminated disposables in aregulated medical waste container.
b. Clean and decontaminate the exterior of theinstrument using a detergent solution followed by a10% chlorine bleach solution or other tuberculocidal
disinfectant. Flush the fluid pathway with chlorinebleach solution as specified in the Operators Manual.
Dispose of waste in accordance with local, state and federalregulations. Probes, needles, broken glass, slides, and othersharps that are contaminated with potentially infectioussubstances should be collected in a sharps container fordisposal as regulated medical waste. Contaminated gloves,wipes, and other disposables should be placed in a standardmedical waste container.
The biohazard icon identifies an activity or area wherepersonnel may be exposed to infectious substances.
The laser hazard icon identifies an activity or areawhere personnel will be exposed to an eye hazard ifprocedural or engineering controls are not observed.
The electrostatic discharge icon identifies an activity orarea where personnel must wear a ground strap whileservicing the system.
:$51,1*= 327(17,$/%,2+$=$5'Considerall clinical specimens, reagent controls, surfaces, or
components that contain or have contacted humanblood or serum as potentially infectious. Wear gloves,lab coats and safety glasses, and follow otherbiosafety practices as specified in the OSHA
Bloodborne Pathogen Rule (29CFR Part 1910.1030)or other equivalent biosafety procedures.
:$51,1*= 327(17,$/%,2+$=$5'Theaspiration needle/probe is sharp and potentiallycontaminated with infectious materials. Avoid contactwith the tip of the needle/probe.
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CELL-DYN3200 SYSTEM SERVICE MANUAL Part Number 9140300August 1998 1 - 7
4 0 ,QWURGXFWLRQ 1.7 Safety
Electrical Safety
Laser Safety
The Class 1 Laser Product Label (Abbott PN 9230702A) isshown below (Figure 1-1). The label consists of black letteringagainst a white background. The label is located on thebackside of the instrument and positioned at a clearly visible
location.
The inner protective cover laser warning labels must not be
removed and are to remain legible. The protective housinglabel (Abbott PN 9230701D) is shown in Figure 1-2. The label
consists of black lettering against a yellow background andappears on the protective cover within theoptics bench and onthe flow panel (see Figure 1-3and Figure 1-4).
Figure 1-1 Laser Product Label (PN 9230702A)
:$51,1*= (/(&75,&$/+$=$5' When theinstrument is powered and protective covers areremoved, there are exposed electrical systems thatcould startle or seriously injure personnel upon
contact. Use appropriate safety precautions to preventbody and/or tool contact with live electricalcomponents, especially power supplies.
DANGER: LASER LIGHT Avoid direct exposure tobeam. Do not look directly into the laser beam or anyreflection of the beam from a mirror-like surface.
When the access door, instrument top cover, or otherinner protective cover is removed, (Helium-Neon)laser power up to 10 mW continuous wave at 632.8nm in a beam with 1 mR divergence could beaccessible in the interior of the optics bench. Thisamount of energy, with insignificant attenuation withdistance, is sufficient to cause eye damage.
&$87,21: Use of controls or adjustments orperformance of procedures other than those specifiedherein may result in hazardous laser light exposure. Ifthe instrument is used or modified in a manner notspecified by the manufacturer, the protection providedby the instrument may be impaired.
32109
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300August 1998 1 - 8
4 0 ,QWURGXFWLRQ 1.7 Safety
Figure 1-2 Laser Warning Label (PN9230702D)
Figure 1-3 Top Laser Warning Label Location
For beam alignment and other open beam configurations,follow instructions provided by the Service Manual, Advisories,and Bulletins regarding the requirement for using laser safety
eyewear. If required, assure laser eyewear are not damagedand have an optical density of 1- 2 at a wavelength of 632.8nm. During open beam configurations, assure that the beam isconfined to the laser bench area and only personnel with propereye protection are present.
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9CTPKPI
.CDGN
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300August 1998 1 - 9
4 0 ,QWURGXFWLRQ 1.8 CELL-DYN 3200 Hematology Parameters
Figure 1-4 Front Laser Warning Label Location
1.8 CELL-DYN 3200 HematologyParameters
The following tables list the CELL-DYN 3200 Hematology
Parameters and their abbreviations.
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9CTPKPI
.CDGN
Table 1-2 Red Cell Parameters
Full Name Abbreviation
Red Blood Cell Concentration RBC
Hemoglobin Concentration HGB
Hematocrit HCT
Mean Cell Volume MCV
Mean Cell Hemoglobin MCH
Mean Cell Hemoglobin Concentration MCHC
Red Cell Distribution Width RDW
Table 1-3 White Cell Parameters
Full Name Abbreviation
White Blood Cell Concentration WBC
Neutrophil Concentration NEUNeutrophil Percentage %N
Lymphocyte Concentration LYM
Lymphocyte Percentage %L
Monocyte Concentration MONO
Monocyte Percentage %M
Eosinophil Concentration EOS
4 , W G WL 1 9 S t S ifi ti
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300August 1998 1 - 10
4 0 ,QWURGXFWLRQ 1.9 System Specifications
1.9 System SpecificationsThe following tables contain the specification for the CELL-DYN3200 System.
Eosinophil Percentage %E
Basophil Concentration BASO
Basophil Percentage %B
Table 1-4 Platelet Parameters
Full Name Abbreviation
Platelet Concentration PLT
Mean Platelet Volume MPV
Platelet-crit PCT*
Platelet Distribution Width PDW*
* Clinical significance has not been established for these
parameters. Therefore they are not reportable in the US.
They are provided for laboratory use only.
Table 1-3 White Cell Parameters (Continued)
Full Name Abbreviation
Table 1-5 Physical Specifications (Unpackaged)
CS Model SL Model GraphicsPrinter
Height 19.0 (48.2 cm) 19.0 (48.2 cm) 7.2 (18.3 cm)
Width 32.8 (83.3 cm) 32.8 (83.3 cm) 16.1 (40.9 cm)
Depth 23.3 (59.2 cm) 29.3 (74.4 cm) 9.9 (25.1 cm)
Weight 214 lb (97.3 kg) 232 lb (105.5 kg) 9.9 lb (4.5 kg)
Table 1-6 Physical Specifications (Packaged)
CS Model SL ModelGraphics
Printer
Height 38 (96.5 cm) 38 (96.5 cm) 12 (30.5 cm)
Width 34 (86.3 cm) 34 (86.3 cm) 19.5 (49.5 cm)
Depth 31 (78.7 cm) 31 (78.7 cm) 14.8 (37.6 cm)
Weight 255 lb (116 kg) 273 lb (124 kg) 16.4 lb (7.4 kg)
4 ,QWURGXFWLRQ 1 9 System Specifications
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4 0 ,QWURGXFWLRQ 1.9 System Specifications
Table 1-7 Analyzer Input Requirements
Module Voltage FrequencyMax
Current
Analyzer
110 VAC + 10 % 50/60 3 Hz 6 amps
120 VAC + 10 % 50/60 3 Hz 6 amps
220 VAC + 10 % 50/60 3 Hz 3 amps
240 VAC + 10 % 50/60 3 Hz 3 amps
Analyzer &
Sample
Loader
110 VAC + 10 % 50/60 3 Hz 6 amps
120 VAC + 10 % 50/60 3 Hz 6 amps
220 VAC + 10 % 50/60 3 Hz 3 amps
240 VAC + 10 % 50/60 3 Hz 3 amps
Monitor 100 - 240 VAC 50/60 1.3 amps
Printer 120 VAC 10% 50/60 0.5 amps220 - 260 VAC
Table 1-8 Performance Specifications
Background Precision CorrelationLinearRange
WOC 0.10K/L 2.7% 0.99 0.0-250 K/ L
NOC 0.10 K/L 2.7% 0.99 0.0-250 K/ L
RBC 0.20 M/L 1.5% 0.98 0.0-8.0 M/ L
HGB 0.1g/dl 1.0% 0.98 0.0-25.0g/dL
MCV N/A 1.0% 0.98 35-180 fL
RDW N/A 5.0% 0.91 N/A
PLT 5.0K/L 4.0% 0.980.0 - 1750
K/L
MPV N/A 5.0% 0.72 2.0-18.0 fL
4 0 ,QWURGXFWLRQ 1 9 System Specifications
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300August 1998 1 - 12
4 0 ,QWURGXFWLRQ 1.9 System Specifications
Table 1-9 Operational Specifications
Operating Environment
Temperature 15o- 30oC (59o- 86oF)
Relative Humidity 20% to 80%, RHNC
Complete Cycle TimesStartup (From Power Off) ~7 minutes
Startup (From Standby) ~5 minutes
Count Cycle, Patient ~52 seconds
Count Cycle + FrgWBC ~100 seconds
Count Cycle + ResRBC ~100 seconds
Shutdown (To Standby) ~130 seconds
Shutdown (To Power Off)~130 seconds
Throughput
CS Model
Open 80 Samples/Hour
Closed 68 Samples/Hour
SL Model
Open 80 Samples/Hour
Closed 68 Samples/Hour
Whole Blood Aspiration Volume
Open Mode 125 L
Closed Mode 250 L
Specimen Tube Dimensions
Diameter 13 mm
Length 75 mm
Minimum Specimen Volumes
Closed and Sample Loader Mode 1 mLOpen Mode 500 L
Batch Size
1 to 50 tubes per batch
Bar Code Formats
Interleaved 2-of-5 code
Codabar
Code 39Code 128 (check digit only)
Bar Code Specifications
Printed on good quality label stock
0.25-inch quiet zone on each end
0.01-inch minimum narrow bar width
0.5-inch minimum bar length
2-inch maximum label length
1.25-inch maximum label width
5 7URXEOHVKRRWLQJ Table of Contents TOC
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300-August 1998 2 - 1
5 7URXEOHVKRRWLQJ Table of Contents
5 7URXEOHVKRRWLQJ
Table of Contents
2.1 Summary. .. . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
2.2 Troubleshooting Approach . . . . . . . . . . . . . 2-2
2.3 Error Messages . . . . . . . . . . . . . . . . . . . . . . . 2-3
IP-1 Fluid Leaks, Open Mode Probe or Wash Block . . . 2-4IP-2 Fluid Leaks, Closed Mode Needle or Wash Block .2-6IP-3 Incomplete Aspiration . . . . . . . . . . . . . . . . . . . . . . . 2-8IP-4 Flow Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-11IP-5 RBC/HGB Diluent Overpressure . . . . . . . . . . . . .2-13IP-6 Optical Parameter Count Problems . . . . . . . . . . .2-15
IP-7 Reagent Empty . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-26IP-8 Optical Backgrounds Too High . . . . . . . . . . . . . . .2-31IP-9 Histogram Problems . . . . . . . . . . . . . . . . . . . . . . .2-32IP-10 Controls Out . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-33IP-11 Vacuum Accumulator #1 Wet . . . . . . . . . . . . . . . .2-35IP-12 Vacuum Accumulator #2 Wet . . . . . . . . . . . . . . . .2-37IP-13 HGB Problems . . . . . . . . . . . . . . . . . . . . . . . . . . .2-39IP-14 X-B Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-41IP-15 Optical Parameters Imprecision . . . . . . . . . . . . . .2-44IP-16 HGB Imprecision . . . . . . . . . . . . . . . . . . . . . . . . .2-47
IP-17 Primary Vacuum/Pressure Isolation, . . . . . . . . . .2-48IP-18 Pressure Leak Isolation . . . . . . . . . . . . . . . . . . . .2-57IP-19 Vacuum Leak Isolation . . . . . . . . . . . . . . . . . . . . .2-64IP-20 Shear Valve Position Fault . . . . . . . . . . . . . . . . . .2-70IP-21 Flags . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-72IP-22 External Waste Full . . . . . . . . . . . . . . . . . . . . . . .2-74IP-23 Blank Display. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-76IP-24 HSSL Failures/Message Reception Time-Out . . . 2-77
IP-25 Hard Drive Errors . . . . . . . . . . . . . . . . . . . . . . . . . 2-78
IP-26 Printer Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-79IP-27 Sample Loader & Aspiration Tower
Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . 2-81Appendix 2A -Cable Connection Diagram . . . . . . . . . . . . 2A-1Appendix 2B -System Block Diagrams . . . . . . . . . . . . . . 2B-1Appendix 2C -System Flow Diagrams . . . . . . . . . . . . . . . 2C-1
TOC
5 0 7URXEOHVKRRWLQJ 2.1 Summary
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CELL-DYN3200 SYSTEM SERVICE MANUAL Part Number 9140300- August 1998 2 - 2
5 7URXEOHVKRRWLQJ 2.1 Summary
2.1 Summary
This chapter contains the following information for CELL-DYN3200:
Error Messages
Isolation Procedures (IPs)
Block Diagrams
Normal Scatter/Histograms
Service Commands
2.2 Troubleshooting Approach
Troubleshooting is required whenever an Observed Error or aDisplayed Error occurs. Displayed Errors can be viewed on the
Bulletin Line or status box of the Data Station screen. (TheBulletin Line is down about 3/4 way on the screen.) Detailedinformation can be found in the fault report screen of thediagnostics submenu.
When a malfunction occurs, follow these recommendedprocedures:
1. Use this Troubleshooting Guide as follows:
a. If possible, begin by verifying the reported error.Perform the exact procedure that produced the errorcondition.
b. When an error occurs, either observed or displayed,look up the error in:
the Error Messagestable or IP 27, Sample Loader & Aspiration Tower Error
Messagestable
c. To refer to the appropriate IP or VP, click on the redlink.
d. Begin at the START box and follow the directions in theIP.
e. If a part is adjusted or replaced, go toSection 5.2,Verification Matrix.
f. Find the part in left column.
g. Perform VPs in order given (i.e., 1, 2, 3, 4 etc.)
2. Perform Total Call.
5 0 7URXEOHVKRRWLQJ 2.3 Error Messages
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CELL-DYN3200 SYSTEM SERVICE MANUAL Part Number 9140300- August 1998 2 - 3
J g
2.3 Error Messages
Error Corrective Action
Background, HGB Too High IP-13
Background, RBC/ PLT Too High IP-8
Background, WBC Too High IP-8Bar Code Reader Failure IP-27
C:\ Hard Drive Errors IP-25
Controls Out IP-10
Diluent/Sheath Empty IP-7
Erratic Results, HGB IP-13
Erratic Results, Optical
IP-6Ensure Blood Sam
ple is Fresh. Differential
count is not reliable
before 30 minutes or after
12 hours after sample is
drawn.
External Waste Full IP-22
Flags IP-21
Flow Errors IP-4
Fluid Leaks, Closed Mode Probe IP-2
Fluid Leaks, Open Mode Probe IP-1
HCT Errors IP-14
HGB Errors IP-13
Histogram Errors IP-9
HSSL Com Failures IP-24
Incomplete Aspiration IP-3
Invalid Tube Height IP-27
Leaks, Pressure IP-18
Leaks, Vacuum IP-19
Load Zone Empty Detector
Malfunction IP-27
MCH Errors IP-14
MCHC Errors IP-14
MCV Errors IP-14
Message Reception Timeout IP-24
Mix Head Not At Top Position IP-27
Mix Head Stuck At Top Position IP-27
Mix Head Stuck In Angle Position IP-27Mix Head Stuck In Vertical
PositionIP-27
Mix Zone Rack Position Error IP-27
No Tube Present IP-27
Overpressure, Diluent Syringe IP-5
Precision Problems HGB IP-16,OPT IP-15
Pressure Errors IP-17
Printer Errors IP-26
Probe Stuck At Home Position IP-27
Probe Unable To Reach Home
PositionIP-27
Rack Bar Code Read Failure IP-27
Shear Valve Position Fault IP-20
Error Corrective Action
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CELL-DYN3200 SYSTEM SERVICE MANUAL Part Number 9140300- August 1998 2 - 3A
J g
Tube Dropped During Mixing IP-27
Tube Lost Moving From Position
3 To 4IP-27
Tube Position Bar Code Read
FailureIP-27
Tube Stuck In Position 3 IP-27
Tube Stuck In Position 4 IP-27
Unexpected Tube In Position 4
After Rack AdvanceIP-27
Unload Area Hardware
MalfunctionIP-27
Unload Zone Full Detector
Malfunction IP-27
Unload Zone Nearly Full Detector
MalfunctionIP-27
Vacuum Accumulator 1 Wet IP-11
Vacuum Accumulator 2 Wet IP-12
Vacuum Errors IP-17
WBC Imprecision
IP-15Ensure Blood Sam-
ple is Fresh. Differential
count is not reliable
before 30 minutes or after
12 hours after sample is
drawn.
WBC/HGB Lyse Empty IP-7
X-B Errors IP-14
Error Corrective Action
5 7URXEOHVKRRWLQJ IP-1 Fluid Leaks, Open Mode Probe or Wash Block
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300-August 1998 2 - 4
IP-1 Fluid Leaks, Open Mode Probe or Wash Block (Page 1 of 2)
Start
Probe LeakingIn Ready Mode
?
- Clamp tubing betweenUltrasonic sensor andShear valve
Wash Block LeakingIn Ready Mode
?
N O
YES
Leaking
Stops?
- Verify connections onUltrasonic Sensor- Verify and tighten fitt ingson Y-Valve- Verify connection on top
of Probe- Replace Y-Valve(PL-8)
YES
- Clamp tubing at the top of
Valve 11
N O
Leaking
Stops
?
YES
- Verify pinch tubing in
Valve 11- Verify and replaceValve 11 (PL-4.1)
- Verify Shear valve
fitt ings- Clean Shear Valve(VP-48)- Replace Shear Valve(PL-9.1)
N O
1
YES
2
N O
5 7URXEOHVKRRWLQJ IP-1 Fluid Leaks, Open Mode Probe or Wash Block
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 5
IP-1 Fluid Leaks, Open Mode Probe or Wash Block (Page 2 of 2)
1
- Verify pinch tubing in
Valve 15- Verify and replace Valve15 (PL-4.1)
2
- Run a several CountCycles while observing thebottom of the Wash Block
Liquid
Leaking
?
- Ensure Vacuum #1 and Pressure #3are within specifications(VP-16)- Ensure bottom f i t t ing on Wash Block
is not restricted- Ensure tubing between Wash Blockand Valve 14 is not restricted- Verify operation of Valve 14 andreplace if necessary(VP-10), (PL-4.1)
- Replace pinch tubing in Valve 14(TM-1)- Ensure tubing between Valve 14 andWC #4 is not restr icted- Verify operation of right Vent Trap
Check Valve and replace i f necessary(TM-1)- Ensure tubng between WC #4 andValve 31 is not restricted- Verify operation of Valve 31 and
replace if necessary(VP-10), (PL-4.1)- Replace pinch tubing in Valve 31(TM-1)
- Ensure f i t t ings on Wash
block are not leaking
N O
YES
5 7URXEOHVKRRWLQJ IP-2 Fluid Leaks, Closed Mode Needle or Wash Block
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 6
IP-2 Fluid Leaks, Closed Mode Needle or Wash Block (Page 1 of 2)
Start
Probe LeakingIn Ready Mode
?
- Clamp tubing betweenUltrasonic sensor andShear valve
Wash Block Leaking
In Ready Mode
?
N O
YES
Leaking
Stops
?
- Verify connections onUltrasonic Sensor
- Verify and tighten fitt ingson Y-Valve- Verify connection on top
of Probe- Replace Y-Valve(PL-8)
YES
- Clamp tubing at the top of
Valve 11
N O
LeakingStops
?
YES- Verify pinch tubing inValve 11
- Verify and replaceValve 11(PL-4.1)
- Verify Shear valvefitt ings- Clean Shear Valve(VP-48)- Replace Shear Valve
(PL-9.1)
N O
1
YES
2
N O
Leave tubing connected, andremove Needle from Wash Block
5 7URXEOHVKRRWLQJ IP-2 Fluid Leaks, Closed Mode Needle or Wash Block
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 7
IP-2 Fluid Leaks, Closed Mode Needle or Wash Block (Page 2 of 2)
1
- Verify pinch tubing in
Valve 34- Verify and replace Valve34 (PL-4.1)
2
- Run a several CountCycles while observing thebottom of the Wash Block
Liquid
Leaking
?
- Ensure Vacuum #1 and Pressure #3 arewithin specifications(VP-16)- Ensure bottom f i t t ing on Wash Block is notrestricted
- Ensure tubing between Wash Block andValve 37 is not restricted
- Verify operation ofValve 37and replace ifnecessary (VP-10), (PL-4.1)- Replace pinch tubing in Valve 37(TM-1)
- Ensure tubing between Valve 37 and WC #4is not restricted- Verify operation of right Vent Trap CheckValve and replace if necessary(TM-1)- Ensure tubng between WC #4 and Valve 31 is
not restricted- Verify operation ofValve 31 and replace ifnecessary (VP-10),(PL-4.1)- Replace pinch tubing in Valve 31 (TM-1)
- Ensure f i t t ings on Wash
block are not leaking
N O
YES
5 7URXEOHVKRRWLQJ IP-3 Incomplete Aspiration
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 8
IP-3 Incomplete Aspiration (Page 1 of 3)
Start
- Aspirate NormalControl in both open
and closed modes.
Mode(s)
Affected
?Open Only
Closed Only
- Verify Y-Valve is rotating
properly (VP-7).
Rotating
Properly
?
- Clean Shear Valve (VP-48).
Problem
Corrected
?
Total Call
YES
NO
- Replace Y-Valve Assy (PL-8) .
- Replace MDM (Pl-9.2)
- Replace SDM/CDM (PL-15).
NO
YES
2 4
Both
1
5 7URXEOHVKRRWLQJ IP-3 Incomplete Aspiration
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 9
IP-3 Incomplete Aspiration (Page 2 of 3)
1
- Run an open cycle andt ime the blood movementto the Ultrasonic Sensor.
Start when Valve 12opens and stop whenblood reaches sensor.
- Clean Open Probe(VP-50).
- Ensure there are norestr ict ions between Open
Probe and Y-Valve.
- Replace Open Probe (PL-8).
< 3.5 Seconds
?
N O
- Place a blood sampleunder Open Probe andmanual ly open Valve 12until the blood is justpast the Y-Valve.
- Verify an air gap ofapproximately 1 inchbetween the diluent andblood columns.
- If no air gap is present,
check the Open Probeplumbing for air leaks.
YES
2
- Run a closed cycle and
time the bloodmovement to theUltrasonic Sensor. Startwhen Valve 12 opens
and stop when bloodreaches sensor.
- Clean Closed Probe(VP-51) .
- Ensure there are no
restr ict ions between ClosedProbe and Y-Valve.
- Replace Closed Probe(PL-8).
- Verify Valve 38(VP-10)an d
ensure Vent Trap plumbing isfree of restrictions.
< 7.0 Seconds
?
N O
- Manually place a blood
sample under Closed
Probe.- From Main Menu, press
D IAGNOSTICS
MORE
TOWER TEST
NEEDLE DOWN
- Manually open Valve 12until the blood is justpast the Y-Valve.
- Verify an air gap ofapproximately 3 inchesbetween the diluent andblood columns.
- Check the Closed
Probe plumbing for airleaks.
Air GapPresent
?
3
YES
NO
YES
5 7URXEOHVKRRWLQJ IP-3 Incomplete Aspiration
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 10
IP-3 Incomplete Aspiration (Page 3 of 3)
- INITIALIZE and PRIMEinstrument.
- Run a closed cycle andverify that the bloodcolumn stops just pastSample Sensor #2.
- If blood does not stop:
- Perform Sample Sensor
adjustment(VP-12).
- Re-seat sensor cable.- Replace Sample
Sensor(PL-9.1).
- Replace SampleSensor Board(PL-9.2).
3 4
- Verify Vac 2 level(VP-16) . Troubleshoot as necessary.
- Ensure there are no restrictions between Y-Valve andValve 12
-Verify Valve 12 and tubing (VP-10). Replace as necessary(PL-4.1),(TM-1) .
- Run an open cycle andt ime the bloodmovement to theUltrasonic Sensor. Startwhen Valve 12 opensand stop when blood
reaches sensor.
< 3.5 Seconds
?
NO
- Run an open cycle andverify that blood column
stops just pastUltrasonic Sensor. Ib lood does not stop:
- Re-seat Ultrasonic
Sensor Cable.
- Replace UltrasonicSensor(PL-8).
- Replace UltrasonicSensor Board (PL-9.2) .
YES
5 7URXEOHVKRRWLQJ IP-4 Flow Errors
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 11
IP-4 Flow Errors (Page 1 of 2)
Start
68
WC 1
SampleStagingPump
Delivery Syringe
From Main Menu:D IAGNOSTICS
MORE
MOTOR OPERATION
EXERCISE MOTORS
- Type A and press Enter.
- Observe the Delivery Syringeand ver i fy smooth upwardmovement (See Figures).
DrippingO K
?
1
N O
YES
- Run a Count Cycle and observethe Del ivery Syr inge and WC1.Verify that when the syringe ismoving up, l iquid is dripping intoWC1 at a fast and constant rate
(See Figures).
SmoothMovement
?
3
N O
2
YES
5 7URXEOHVKRRWLQJ IP-4 Flow Errors
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 12
IP-4 Flow Errors (Page 2 of 2)
1
- Verify level of 9 psi (VP-16)
- Verify tubing and operation ofValves 64, 65, 91, and 56 and
replace as necessary(TM-1) ,(PL-4.1).
- Perform Optical Flow Cell
Cleaning Procedure(VP-44).
- Replace In-Line Sheath Filter(PL-4.2).
3
- Remove and clean Del iverySyringe.
- Replace Delivery Syringe
(PL-7).
- Replace Syringe DriverAssembly (PL-7).
- Replace MPM(PL-11).
2
- Check Laser for stableoutput (VP-46).
OutputStable
?
- Replace Laser (PL-10).
- Replace Laser Power Supply(PL-2.2).
N O
- Remove, inspect, and cleanFlow Cell Nozzle.
- Replace Flow Cel l Assy. (PL-10).
YES
5 7URXEOHVKRRWLQJ IP-5 RBC/HGB Diluent Overpressure
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 13
IP-5 RBC/HGB Diluent Overpressure (Page 1 of 2)
Start
- Check resistance ofOverpressure Sensor
SensorShorted
?
YES
- Replace Sensor (PL-4.2)
- Remove, inspect, and clean
Shear Valve(VP-48)
- Ensure di luent paths fromsyringe to RBC and HGBMixing Chambers are free of
restrictions
- Verify operation of Valves 27and 26 (V P-10) and replace if
necessary (PL-4.1)
N O
Prob lemCorrected
?
YES
- Verify Shear Valve rotation time
is
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 14
IP-5 RBC/HGB Diluent Overpressure (Page 2 of 2)
1 2
Total Call
In Ready Mode, try to manuallyrotate the front and back of the
Shear Valve.
I f there is movement:
- Replace defective section:
Front (PL-9.1)
Rear (PL-9.1)If problem persists:
- Replace Shear Valve Driver(PL-9.1)
- Lubricate Shear Valve Driver(VP-42)
If problem persists:
- Replace Shear Valve Driver(PL-9.1)
Total Call
32002
Rim NotchFaces Right
Shear Valve Sect ions
Should Not Move
5 7URXEOHVKRRWLQJ IP-6 Optical Parameter Count Problems
IP 6 O ti l P t C t P bl (P 1 f 11)
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 15
IP-6 Optical Parameter Count Problems (Page 1 of 11)
Start
Note: This procedure assumes that HGB Results are OK in Open and Closed Modes, and there are no hardware or software Error Messages.
I t is designed to f ind and correct gross WBC, NOC, RBC, and PLT problems. For Optical Precis ion Problems see IP-15.
All " 0"
?
From Main Menu, press
RUN
SPECIMEN TYPE
FRAGILE WBC
- Run a Normal Control f ive t imes andrecord the optical values.
- Compare the results to the Data Sheet.
RBC & PLTOnly " 0"
?
N O
3YES
N O
WBC Only " 0"
?
4YES
5YES
RBC Only " 0"
?
PLT Only "0"
?
NO
NOC Only " 0"
?
6YES
8
7YES
YES
1
NO
NONO
- Verify proper Reagents and ExpirationDate.
- Perform Background Count Veri f icat ion(VP-56).
BackgroundsO K
?
YES
Go to(IP-8),correct andreturn here.
N O
5 7URXEOHVKRRWLQJ IP-6 Optical Parameter Count Problems
IP 6 O ti l P t C t P bl (P g 2 f 11)
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 16
IP-6 Optical Parameter Count Problems (Page 2 of 11)
1
All Low
?
RBC & PLT
Only Low?
N O
9YES
N O
WBC OnlyLow
?
10YES
YES
NOC OnlyLow
?
12YES WBC Only
High?
15YES
All High
?
13YES
N O
NOC OnlyHigh
?
16YES
RBC & PLT
Only High
?
14YES
N O
11
2
N O
NO
N O
5 7URXEOHVKRRWLQJ IP-6 Optical Parameter Count Problems
IP 6 Optical Parameter Count Problems (Page 3 of 11)
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 17
IP-6 Optical Parameter Count Problems (Page 3 of 11)
2
All Erratic
?
RBC & PLT
Only Erratic
?
N O
17YESWBC Only
Erratic
?
18YES
YES
NOC OnlyErratic
?
20YES
19
Total Call
N O
N O
N O
5 7URXEOHVKRRWLQJ IP-6 Optical Parameter Count Problems
IP-6 Optical Parameter Count Problems (Page 4 of 11)
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 18
IP-6 Optical Parameter Count Problems (Page 4 of 11)
3
- Veri fy samples are being staged toOptical Flow Cell by Peri-pump.
Troubleshoot as necessary.
- Verify Sheath Reservoir is fi l l ingproperly.
- Verify Pressure 2 (VP-16)an dproper Sheath f low to Optical FlowCel l . Troubleshoot as necessary.
- Replace In-Line Filter(PL-4.2).
- Verify operation of Samp leInjection Syringe(VP-6),(VP-7).
Troubleshoot as necessary.
Prob lemCorrected
?
- Verify laser power(VP-46).
N O
Total Call
YES
Power OK
?
- Connect new Laser Head to
Laser Power Supply andveri fy power (see Warning).I f good, replace Laser Head(PL-10). If not, replaceLaserPower Supply (PL-2).
NO
N O
- Veri fy beam al ignment(VP-20), (VP-21).
- If not able to get data onthe Extended Count
Screen, replace in orderthe Optical Flow Cell,MAM , SPM, and CPU/DCM (PL-10), (PL-12),(PL-13).
YES
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5 7URXEOHVKRRWLQJ IP-6 Optical Parameter Count Problems
IP-6 Optical Parameter Count Problems (Page 5 of 11)
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 19
IP 6 Optical Parameter Count Problems (Page 5 of 11)
4
- Veri fy RBC/PLT Sample is beingstaged to Optical Flow Cell.
- Verify operation of Valve 54 (VP-
10) and tubing. Replace asnecessary(PL-4.1),(TM-1).
- From Main Menu, press
D IAGNOSTICS
F 12
F 1SET POINT ENTRY
- Veri fy RBC/PLT Threshold is550.
- Reseat Preamp, MAM, SPM,CPU/DCM Cables .
- Replace MAM (PL-12).
- Replace 10 oPreamp(PL-10).
- Replace SPM(PL-10).
- Replace CPU/DCM(PL-13).
5
- Veri fy WBC Sample is being
staged to Optical Flow Cell.
- Verify operation of Valve 53and 55(VP-10)and tubing.Replace as necessary(PL-
4.1) ,(TM-1) .
- Veri fy operat ion of WBCSyringe(VP-6), (VP-7).
- From Main Menu, press
D IAGNOSTICS
F 12
F 1
SET POINT ENTRY
- Veri fy WBC Threshold is
360.
- Reseat Preamp, MAM, SPM,CPU/DCM Cables .
- Replace MAM (PL-12).- Replace 0oPreamp(PL-10).
- Replace SPM(PL-10).
- Replace CPU/DCM(PL-13).
- Replace MAM(PL-12).- Replace SPM(PL-10).
- Replace CPU/DCM (PL-13).
6
- Replace MAM(PL-12).- Replace SPM(PL-10).
- Replace CPU/DCM(PL-13).
7
8
- Veri fy NOC Sample is
being staged to OpticalFlow Cell.
- Verify operation of Valve
41(VP-10)and tubing.Replaceas necessary(PL-4.1),(TM-1) .
- From Main Menu, press
D IAGNOSTICS
F 12
F 1SET POINT ENTRY
- Veri fy WBC Threshold is400.
- Perform NOC GainAdjustment(VP-25).
5 7URXEOHVKRRWLQJ IP-6 Optical Parameter Count Problems
IP-6 Optical Parameter Count Problems (Page 6 of 11)
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 20
IP 6 Optical Parameter Count Problems (Page 6 of 11)
9
-- Verify Sheath Reservoir is fi l l ingproperly.
- Verify Pressure 2 (VP-16)an d
proper Sheath flow to Optical FlowCel l . Troubleshoot as necessary.
- Replace In-Line Filter(PL-4.2).
- Verify operation of SampleInjection Syringe(VP-6),(VP-7).Troubleshoot as necessary.
- Replace Vent Accumulator Fi l ter(PL-4.2) .
Prob lemCorrected
?
- Verify laser power (VP-46).
N O
Total Call
YES
Power OK
?
- Connect new Laser Head to
Laser Power Supply andveri fy power (See Warning).I f good, replace Laser Head(PL-10).If not, replaceLaser Power Supply (PL-2).
N ON O
- Clean Laser Bench(VP-43).
- Clean, debubble,and wetOptical Flow Cell(VP-44),(VP-45).
- Verify beam alignment (VP-20 ),(VP-21).
- Replace Optical Flow Cell (PL-
10 ).
- Replace MAM(PL-12).
- Replace CPU/DCM(PL-13).
YES
'DQJHU'DQJHU'DQJHU'DQJHU= +LJK9ROWDJH00 $VVXUHWKHSRZHUWRWKHLQVWUXPHQWLVRII1 $KLJKYROWDJHFKDUJHPD\UHPDLQRQWKHODVHU
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5 7URXEOHVKRRWLQJ IP-6 Optical Parameter Count Problems
IP-6 Optical Parameter Count Problems (Page 7 of 11)
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 21
p ( g )
- Veri fy RBC/PLT Sample isbeing staged to OpticalFlow Cell.
- Verify operation of Valve 54
(VP-10) and tubing.Replace as necessary (PL-4.1),(TM-1).
- Clean Shear Valve(VP-48).
10
- From Main Menu, press
D IAGNOSTICS
F 12
F 1
SET POINT ENTRY
- Veri fy RBC/PLT Thresholdis 550.
- Verify 0oand 10oL ogAmplifiers (VP-28).
- Verify 0oand 10oGain(VP-26 ),(VP-27).
- Replace MAM (PL-12).
- Replace SPM (PL-10).
- Replace CPU/DCM(PL-13).
Prob lemCorrected
?
Total Call
YES
N O
- Veri fy WBC Sample isbeing staged to OpticalFlow Cell.
- Verify operation of Valve 53and 55(VP-10)and tubing.Replace as necessary (PL-4.1) ,(TM-1) .
- Veri fy operat ion of WBCSyringe (VP-6), (VP-7).
- Clean Shear Valve(VP-48).
- From Main Menu, press
D IAGNOSTICS
F 12
F 1
SET POINT ENTRY
- Veri fy WBC Threshold is
360.
- Veri fy WBC Gain(VP-23).
- Replace MAM(PL-12).- Replace SPM(PL-10).
- Replace CPU/DCM(PL-13).
11
Prob lemCorrected
?
Total Call
YES
N O
5 7URXEOHVKRRWLQJ IP-6 Optical Parameter Count Problems
IP-6 Optical Parameter Count Problems (Page 8 of 11)
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 22
p ( g )
12 13
- Clean Laser Bench (VP-43).
- Clean, debubble, and wetOptical Flow Cell (VP-44),
(VP-45).
- Verify beam alignment (VP-20 ),(VP-21).
- Replace Optical Flow Cell(PL-10).
- Replace MAM(PL-12).
- Replace CPU/DCM(PL-13).
14
- From Main Menu, press
D IAGNOSTICS
F 12
F 1
SET POINT ENTRY
- Veri fy RBC/PLT Threshold is
550.
- Verify 0oand 10o Log Amplifiers
(VP-28).- Verify 0oand 10oGain(VP-26),
(VP-27).
- Replace MAM(PL-12).
- Replace SPM (PL-10).
- Replace CPU/DCM(PL-13).
- Veri fy NOC Sample is beingstaged to Optical Flow Cell.
- Verify operation of Valve 41(VP-10)and tubing. Replace asnecessary(PL-4.1), (TM-1) .
- From Main Menu, press
D IAGNOSTICS
F 12
F 1
SET POINT ENTRY
- Veri fy NOC Threshold is 400.
- Perform NOC Gain Adjustment(VP-25).
5 7URXEOHVKRRWLQJ IP-6 Optical Parameter Count Problems
IP-6 Optical Parameter Count Problems (Page 9 of 11)
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 23
16
- Veri fy operat ion of WBCSyringe(VP-6),(VP-7).
- Clean Shear Valve(VP-48).
- From Main Menu, press
D IAGNOSTICS
F 12
F 1
SET POINT ENTRY
- Veri fy WBC Threshold is360.
- Veri fy WBC Gain(VP-23).
- Replace MAM(PL-12).
- Replace SPM(PL-10).- Replace CPU/DCM(PL-13).
15
Prob lemCorrected
?
Total Call
YES
N O
- From Main Menu, pressD IAGNOSTICS
F 12
F 1
SET POINT ENTRY
- Veri fy NOC Threshold is400.
- Perform NOC Gain
Adjustment(VP-25).
5 7URXEOHVKRRWLQJ IP-6 Optical Parameter Count Problems
IP-6 Optical Parameter Count Problems (Page 10 of 11)
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 24
17
- Veri fy samples are being staged toOptical Flow Cell by Peri-pump.Troubleshoot as necessary.
- Verify Sheath Reservoir is fi l l ingproperly.
- Verify Pressure 2 (VP-16)an dproper Sheath flow to Optical FlowCel l . Troubleshoot as necessary.
- Replace In-Line Filter(PL-4.2).
- Replace Vent Accumulator Fi l ter(PL-4.2).
- Veri fy operat ion of SampleInjection Syringe(VP-6),(VP-7).Troubleshoot as necessary.
Prob lemCorrected
?
N O
Total Call
YES
- Verify stable laser power
(VP-46).
Power Stable
?
- Connect new Laser Head toLaser Power Supply andveri fy power (See Warning).I f good, replace Laser Head
(PL-10). If not, replace LaserPower Supply(PL-2).
N O
- Clean Laser Bench(VP-43).
- Clean, debubble, and wet
Optical Flow Cell(VP-44),(VP-45).
- Replace Optical Flow Cell
(PL-10).
- Replace MAM(PL-12).
- Replace SPM(PL-12).
- Replace CPU/DCM(PL-13).
YES
N O
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5 7URXEOHVKRRWLQJ IP-6 Optical Parameter Count Problems
IP-6 Optical Parameter Count Problems (Page 11 of 11)
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 25
- Verify RBC/PLT Sample is being
staged to Optical Flow Cell.
- Verify operation of Valve 54 (VP-10)
and tubing. Replace as necessary
(PL-4.1) ,(TM-1) .
- Clean Shear Valve (VP-48) .
18
- From Main Menu,
press
DIAGNOSTICS
F 12
F 1
SET POINT ENTRY
- Verify RBC/PLT
Threshold is 550.
Problem
Corrected
?
Total Call
YES
NO
- Verify operation of WBC
Syringe (VP-6) ,(VP-7) .
- Clean Shear Valve (VP-48).
- From Main Menu, press
DIAGNOSTICS
F 12F 1
SET POINT ENTRY
- Verify WBC Threshold is
360.
- Replace MAM (PL-12) .
- Replace SPM (PL-10) .
- Replace CPU/DCM (PL-13) .
19
Problem
Corrected?
Total Call
YES
NO
20
- Verify NOC Sample is being staged
to Optical Flow Cell.
- Verify operation of Valve 41 (VP-10)
and tubing. Replace as necessary
(PL-4.1) ,(TM-1).
- From Main Menu, press
DIAGNOSTICS
F 12
F 1
SET POINT ENTRY
- Verify NOC Threshold is 400.
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 26
Start
- Verify reagents, type andexpiration.
- Ensure reagent
containers are connected
to the correct tubing andinput ports.
- Ensure sinkers are on
lines and lines are at
bottom of containers.
- Ensure reagent lines arenot crimped or clogged.
- INITIALIZE and PRIME
instrument.
HGB LyseEmpty
?
WBC Lyse
Empty
?
NO
Diluent/Sheath
Empty
?
NO
Total Call
NO
- Open Right Front Door
and observe HGB/
WBC Syringe.
- Open Left Front Door
and observe Diluent andSheath Reservoirs.
YES
YES
YES
2
1
5 7URXEOHVKRRWLQJ IP-7 Reagent Empty
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 27
1
From Main Menu,press
RUN
CLEAR FAULT
SyringeMoving
Properly?
SyringeFill ing
Properly
?YES
- Clean/replace syringe(PL-7).
- Verify Syringe Drive roperat ion(VP-6),(VP-7).
- Replace Syringe DriverAssembly (PL-7),(RR-11) .
- Replace MPM(PL-11).
N O
- Verify operation and tubingin valves 28 for HGB, 23for WBC. (VP-10)S eeFigure. Replace as
necessary(PL-4.1),(TM-1) .- Replace SDM2(PL-15)
- Verify operation and tubing invalves 24 for HGB, 25 for WBC.(VP-10)See Figure. Replace as
necessary(PL-4.1),(TM-1).- Replace SDM2(PL-15).
N O
- Remove Top Cover andobserve Reagent Sensor
Board. See Figure
- Inspect and seatconnectors on J1 ofReagent Sensor Board
and J26 of FCM.
- Replace FCM(PL-14.1).
YES
Floss TubingUp and DownEnsure Tubingis Seated in Slot
32098
5 7URXEOHVKRRWLQJ IP-7 Reagent Empty
IP-7 Reagent Empty (Page 3 of 5)
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 28
2
From Main Menu, pressRUN
CLEAR FAULT
- Observe the Di luent and SheathReservoirs and verify that both fi l l tothe Sensor Electrodes andimmediately stop. See Figure
DiluentNot Fil l ingProperly
?
Sheath
Not Fil l ingProperly
?
Diluent Fil l ingPast Electrode
?
N O
N O
Sheath Fil l ingPast Electrode
?
N O
5
Note: The "Di luent/Sheath Empty"message can be generated by the In- l ine
Di luent/Sheath Sensor, the Di luent
Reservoir Level Sensor, or the SheathReservoir Level Sensor.
YES
6YES
7YES
BothFill ing
Properly?
4
Neither
Fill ingProperly
?
N O
YES
N O
3YES
YES
32099
Fill
ToHere
5 7URXEOHVKRRWLQJ IP-7 Reagent Empty
IP-7 Reagent Empty (Page 4 of 5)
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 29
3
- Remove Top Cover andobserve Reagent SensorBoard. See Figure
- Inspect and seatconnectors on J1 ofReagent Sensor Boardand J26 of FCM.
- Replace FCM(PL-14.1).
4
- Ensure Di luent/Sheath plumbing
is free of restrictions.
- Ensure there is no saline bridge atreservoir connectors.
- Verify the Vac 1 level(VP-16)and verify Vac 1 retention (VP-15 ).
- Ensure Vac 1 lines to reservoirs isfree of restrictions.
- Verify operation of Valves 61, 62,63, and 64(VP-10).Replace asnecessary(PL-4.1).
- Verify operation of Check Valves.
Replace as necessary (TM-1).
- Verify Reservoirs are not crackedor leaking. Replace as necessary
(PL-4.2).
- Replace FCM(PL-14.1).
5
- Ensure Vac 1 line to reservoiris free of restrictions.
- Ensure there is no salinebridge at reservoir connector.
- Verify operation of Valves 62and 63 (VP-10). Replace asnecessary (PL-4.1)
- Verify operation of CheckValve Replace as necessary(TM-1) .
- Verify Reservoir is not
cracked or leaking Replaceas necessary(PL-4.2).
- Replace FCM(PL-14.1).
6
- Ensure Vac 1 line toreservoir is free ofrestrictions.
- Ensure there is no salinebridge at reservoirconnector.
- Verify operation of Valves 61
and 64(VP-10).Replace asnecessary(PL-4.1).
- Verify operation of CheckValve. Replace asnecessary(TM-1).
- Verify Reservoir is notcracked or leaking. Replace
as necessary (PL-4.2).
- Replace FCM(PL-14.1).
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 30
7
32100
- Clean sensor contacts
(Electrodes andconnector) at top of
Reservoir .
- Remove pins 1 and 4and clean and cr imp
metal contacts so theyare touching. See Figure
- If pins 1 and 4 are
damaged, replace withpins 2 and 3.
- Reseat Sensor Cables(CDM 2 J8 for Diluent, or
CDM 2 J10 for Sheath.
- Replace FCM (PL-14.1).
5 7URXEOHVKRRWLQJ IP-8 Optical Backgrounds Too High (Page 1 of 1)
IP-8 Optical Backgrounds Too High (Page 1 of 1)
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Start
WBC OnlyToo High
?
- Veri fy Background Counts
(VP-56).
- From Main Menu, press
D IAGNOSTICS
F 12
F 1
SET POINT ENTRY
- Veri fy WBC Threshold is 360.
- Replace WBC Lyse Container.
- Clean/replace WBC Syringe(PL-7).
- Ensure WBC Mixing Chamber isbeing rinsed and drained properly.
- INITIALIZE and PRIME Instrument.
YES
- From Main Menu, press
D IAGNOSTICS
F 12F 1
SET POINT ENTRY
- Veri fy RBC/PLT Threshold is 550.
- From Main Menu, press
SPECIAL PROTOCOLS
MORE
REAGENT RESERVOIR
- Follow on-screen instructions and press
EMPTY DIL/SHEATH
- Replace Di luent Container and press
FILL DIL/SHEATH
- Run multiple backgrounds and verify.
Backgrounds
O K
?
Total Call
YES
N O
- Perform the following in order and verifybackgrounds after each act ion taken.
- Perform Extended Autoclean.
- Clean Shear Valve(VP-48).
- Clean and debubble Optical Flow Cel l(VP-44).
- Clean/replace Di luent and Sample
Injection Syringes(PL-7).
- Replace In-line Sheath Filter(PL-4.2).
- Ensure RBC/PLT Mixing Chamber isbeing rinsed and drained properly.
- Clean Laser Bench(VP-43).
N O
5 7URXEOHVKRRWLQJ IP-9 Histogram Problems (Page 1 of 1)
IP-9 Histogram Problems (Page 1 of 1)
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 32
Start
- Verify reagents; type andexpiration date.
- Ensure reagents are atroom temperature.
- Veri fy Backgrounds(VP-56).
Prob lem
Corrected
?
Total Call
YES
- Perform the following in order
and verify histograms aftereach act ion taken.
- Ensure RBC/PLT and/orWBC Mixing Chambers are
being r insed and drainedproperly.
- Perform Extended Autoclean.
- Clean Shear Valve (VP-48).
- Clean, debubble,and wet
Optical Flow Cell (VP-44),(VP-45).
- Fine align Optical Flow Cell
(VP-20).
- Clean Laser Bench(VP-43).
- Clean/replace Di luent, WBCLyse, and Sample Inject ion
Syringes (PL-7).
- Replace In-line Sheath Filter(PL-4.2).
WBC Only
?
N O
Prob lemCorrected
?
Total Call
N O
- Verify 0oand 10oL ogAmplifiers (VP-28).
- Verify 0oand 10oGains(VP-26),(VP-27).
- Replace WBC Lyse for WBC
problem or Di luent for RBC/PLT problem.
BackgroundsOK
?
YES
Go to(IP-8)
N O N O
- Veri fy WBC Gains(VP-23).
YES
YES
5 7URXEOHVKRRWLQJ IP-10 Controls Out
IP-10 Controls Out (Page 1 of 2)
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 33
Start
- Verify reagents; type andexpiration date.
- Ensure reagents are at roomtemperature and have not
been previously frozen.
- Ensure Controls andCal ibrators have not been
frozen or heat stressed.
- Check Control Lot #
- Use current Control InsertSheet to ver i fy Q.C means/l imits are correct if customerhas not establ ished their ownmeans.
- From Main Menu, press
SETUP
Q.C. SETUP MENUQ.C. LIMITS
- Edit as necessary.
- Run Q.C.
Q.C. OK
?
- Perform Autoclean.
- From Main Menu, press
SPECIAL PROTOCOLS
MORE
AUTOCLEAN
- Veri fy Backgrounds(VP-56).
Total Call
- Open fresh CommercialControl , and run Q.C.
Al lBackgrounds
O K?
Go to(IP-8) .
ResultsAcceptable
?
Total Call
1
N O
YES
YES
N O
YES
N O
5 7URXEOHVKRRWLQJ IP-10 Controls Out
IP-10 Controls Out (Page 2 of 2)
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 34
1
- Clean Shear Valve(VP-48).
- Recalibrate out-of-
range parameter(s)(VP-58).
- Verify System Precision
- From Main Menu, press
RUN
SPECIMEN TYPE
- Select empty Q.C. Fi le
Q.C. SPECIMEN
- Review CV%(VP-57).
Precis ionIn Spec
?
YES
- Determine which
parameter(s) are out-of-range, and go to(IP-15)or ( IP-16) for HGB andcorrect.
- For indices (MCH or
MCHC), determine whichparameter (HGB, MCV,or RBC is imprecise andtroubleshoot accordingly.
MCH = HGB X10
RBC
MCHC = HGB X 100
MCV X RBC
NO
5 7URXEOHVKRRWLQJ IP-11 Vacuum Accumulator #1 Wet
IP-11 Vacuum Accumulator #1 Wet (Page 1 of 2)
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 35
Start
- Reinitialize AnalyzerFrom Main Menu:
D IAGNOSTICS
MORE
INITIALIZATION
- Veri fy that Waste Chamber 2 isnot overflowing. If overflowing,verify Pressure Valve 18(VP-10).
Replace as necessary (PL-4.1).Check output Check Valve.Replace as necessary (TM-1) .
- Ensure operat ion of WasteChamber 2 Trap and Trap OutputCheck Valve. Replace asnecessary (PL-4.2)and (TM-1) .
Liquid ExitingAnalyzer
?
Press:
MORE
- Observe the Waste Line intoExternal Waste Container andpress:
DRAIN ACCUMULATORS
1
- Perform Vacuum
Accumulator #2 RinsingProcedure (VP-60)
N O
Prob lemCorrected
?
Total Call
YES
N O
YES
- Perform VacuumAccumulator #2 RinsingProcedure (VP-60)
5 7URXEOHVKRRWLQJ IP-11 Vacuum Accumulator #1 Wet
IP-11 Vacuum Accumulator #1 Wet (Page 2 of 2)
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 36
1
- Remove connector fromJ11 on VPM and checkresistance betweenconnector pins. Must be
greater than 20Megohms. See Figure
> 20 Megohms
?
- Replace VPM(PL-5)
- Replace FCM(PL-14.1)
YES
- Check Cable and replace
if necessary(PL-5)
- Replace Accumulator(PL-5)
NO
V P MB D
J1 0Vac 1 Acc
J1 1Vac 2 Ac
32003
5 7URXEOHVKRRWLQJ IP-12 Vacuum Accumulator #2 Wet
IP-12 Vacuum Accumulator #2 Wet (Page 1 of 2)
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 37
Start
- Reinitialize Analyzer
From Main Menu:
D IAGNOSTICS
MORE
INITIALIZATION
- Veri fy that Waste Chamber 2 is
not overflowing. If overflowing,verify Pressure Valve 18(VP-10).Replace as necessary(PL-4.1).Check output Check Valve.Replace as necessary (TM-1) .
- Ensure operat ion of WasteChamber 2 Trap and Trap OutputCheck Valve. Replace as
necessary(PL-4.2)an d(TM-1) .
Liquid ExitingAnalyzer
?
Press:
MORE
- Observe the Waste Line intoExternal Waste Container and
press:
DRAIN ACCUMULATORS
1
- Perform VacuumAccumulator #2 RinsingProcedure (VP-60)
N O
Prob lemCorrected
?
Total Call
YES
N O
YES
- Perform VacuumAccumulator #2 Rinsing
Procedure (VP-60)
5 7URXEOHVKRRWLQJ IP-12 Vacuum Accumulator #2 Wet
IP-12 Vacuum Accumulator #2 Wet (Page 2 of 2)
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 38
1
- Remove connector fromJ11 on VPM and checkresistance betweenconnector pins. Must be
greater than 20Megohms. See Figure
> 20 Megohms
?
- Replace VPM(PL-5)
- Replace FCM(PL-14.1)
YES
- Check Cable and replace
if necessary (PL-5)
- Replace Accumulator(PL-5)
NO
V P MB D
J1 0Vac 1 Acc
J1 1Vac 2 Ac
32003
5 7URXEOHVKRRWLQJ IP-13 HGB Problems
IP-13 HGB Problems (Page 1 of 2)
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 39
Start
- Veri fy proper Reagents andExpiration Date.
From Main Menu, Press:
RUN
SPECIMEN TYPE
FRAGILE WBC
- Run a Normal Control fivet imes and record the NOC
and HGB values.
- Compare the NOC and HGBresults to the Data Sheet.
"0", Low, High, orErratic
?
- Remove, inspect, and clean Shear
Valve.
- Perform Vacuum & Pressure Leve lVerification/Adjustment (VP-16).
- Verify tubing and operation of Valves51, 28, 24, 33, 93, and 26(VP-10)and replace as necessary (PL-4.1).
- Verify operation of HGB Lyse andRBC Di luent Syr inges(VP-6),(VP-7)and replace as necessary (PL-7).
- Perform HGB CleaningProcedure(VP-49).
From Main Menu, Press:
D IAGNOSTICS
RAW DATA SUMMARY
- Run f ive background countsand observe the HGB Sampleand Reference Readings.
Both
HGB Only
1
5 7URXEOHVKRRWLQJ IP-13 HGB Problems
IP-13 HGB Problems (Page 2 of 2)
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 40
1 2
Total Call
- Perform HGB Adjustment
(VP-5)
3
4
- Verify tubing, operation (VP-10),and f low through Valve 94 andreplace as necessary (TM-1) ,(PL-4.1).
5
- Verify connector at J18 of FCM.
- Replace HGB Flow Cel l (PL-4.2).
- Replace FCM (PL-14.1).
- Replace CPU/DCM (PL-13).
Select path from Chart below.
Reference Sample
1 95 0- 20 50 1 95 0- 20 50
< 1950 < 1950
> 2050 >2050
Errat ic 1950-2050
1600 1950-2050
Errat ic Erratic
0 0
2
3
3
4
5
4
5
5 7URXEOHVKRRWLQJ IP-14 X-B Errors
IP-14 X-B Errors (Page 1 of 3)
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 41
RBC RBC MC VM CV HG B HGB
NA NA NANA
NANA
X-B Parameter
MCV
M CH
M C H C
Effect Of RBC, MCV, & HGB On X-B ParametersStart
- Clean Fan Filters and verify all Fans are runningproperly.
- Ensure there is at least 6" of clearance behind andon the left side of Instrument.
- Ensure Instrument is not subject to drastictemperature variations (direct sunlight, heating/airconditioning ducts).
- If Fan Filters were clogged and then cleaned, allow a15 minute temperature stabilization period.
- Review patient result in Datalog, and look for groups( 3 or more) of abnormal MCV, RBC, or HGB.
-I f MCV shif ts, ask Customer for possible causes,such as Oncology or Geriatric Patients.
I f MCH or MCHC errors:
- See table to determine which parameter (HGB,RBC, MCV) is bad.
Note: HCT = RBC X MCV/10
MCH = HGB X 10/RBC
MCHC = (HGB X 100)/(RBC X MCV)
- Review Q.C. Logs for all 3 Control Levels.
- Veri fy RBC, HGB, and MCV Control Levels are notat the high or low end of range.
Controls at orNear 2 SD
of mean
?
Go to (IP-10)
Controls Out.
YES
- Review Customer Maintenance Log.
- Review Maintenance Procedures andDates.
- Review X-B Results.
- Determine i f Maintenance wasperformed just before X-B Shift.
N O
1
5 7URXEOHVKRRWLQJ IP-14 X-B Errors
IP-14 X-B Errors (Page 2 of 3)
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 42
1
Shift
After
Maintenance
?
- Repeat Maintenance
Proceduresperformed before X-B
Shift.
- Monitor X-B for 5 DataPoints (100 Samples).
YES
X-B In
Range
?
Total Call
YES
- Verify Reagent, Type and
Expiration Date.
Reagent
Change
Required?
2
NO
- Monitor X-B for 5
Data Points (100
Samples).
X-B In
Range
?
YES
Total Call
YES 2
NO
5 7URXEOHVKRRWLQJ IP-14 X-B Errors
IP-14 X-B Errors (Page 3 of 3)
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 43
2
- Verify System Precision
- From Main Menu, press
R UN
SPECIMEN TYPE
- Select empty Q.C. File
Q.C. SPECIMEN
- Run one Patient 10
times.
- Review CV% (VP-57) .
Al l
Parameters
OK?
- Verify Calibrator Insert
Sheet matches Current
Calibrators.
- Perform SystemCalibration (VP-58) .
- Determine which
parameter(s) are out-of-
range, and go to (IP-15) or
(IP-16) for HGB and correct.
NO
YES
5 7URXEOHVKRRWLQJ IP-15 Optical Parameters Imprecision
IP-15 Optical Parameters Imprecision (Page 1 of 3)
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 44
Start
- Clean Fan Filters and verify all Fans are
running properly.
- Ensure there is at least 6" of clearancebehind and on the left side of Instrument.
- Ensure Instrument is not subject to drastictemperature variations (direct sunlight,heating/air conditioning ducts).
- If Fan Filters were clogged and thencleaned, al low a 15 minute temperaturestabilization period.
- Perform Autoclean
From Main Menu, press
SPECIAL PROTOCOLS
MORE
AUTOCLEAN
- Clean Shear Valve(VP-48).- Manually clean, debubble, and wet Optical
Flow Cell (VP-44),(VP45) .
- Clean Optics Bench(VP-43).
- Replace In-line Sheath Filter (PL-4.2).
- Verify System Precision.
- From Main Menu, press
RUN
SPECIMEN TYPE
- Select empty Q.C. File
Q.C. SPECIMEN
- Run one Pat ient 10 t imes.
- Review CV%(VP-57).
Prob lem
Corrected?
Total Call
YES
Al l
Imprecise
?
N O
3
1
Backgrounds
OK
?
- Veri fy Background Counts
(VP-56).
YES
Go to(IP-8).N O
2
RBC/PLTOnly
?
N O
N O
YES
YES
5 7URXEOHVKRRWLQJ IP-15 Optical Parameters Imprecision
IP-15 Optical Parameters Imprecision (Page 2 of 3)
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 45
1
W B C
Only
?
NOC
Only
?
NO
- Ensure HGB Mixing
Chamber is being r insed
and drained properly.
Total Call
NO
YES
- Verify samples are being staged to
Optical Flow Cell by Peri-pump.Troubleshoot as necessary.
- Verify Sheath Reservoir is fil l ing
properly.
- Verify Pressure 2 (VP-16)an d
proper Sheath flow to Optical FlowCell. Troubleshoot as necessary.
- Verify operation of Sample
Injection Syringe(VP-6) ,(VP-7).
Troubleshoot as necessary.- Replace Optical Flow Cell (PL-10) .
- Replace Shear Valve Sections(PL-9.1) .
- Replace MAM (PL-12).
- Replace SPM (PL-10).
- Replace CPU/DCM (PL-13).
- Replace WBC Lyse Container.
- From Main Menu, press
DIAGNOSTICSF 12
F 1
SET POINT ENTRY
- Verify WBC Threshold is 360.
- Ensure WBC Mixing Chamber is
being rinsed and drainedproperly.
- Verify operation of WBC Lyse
Syringe (VP-6) ,(VP-7) .Troubleshoot as necessary.
- Verify/adjust WBC Gain (VP-23).
- Replace Shear Valve Sections
(PL-9.1) .
- Replace MAM (PL-12).
- Replace SPM (PL-10).
- Replace CPU/DCM (PL-13).
YE S
2
5 7URXEOHVKRRWLQJ IP-15 Optical Parameters Imprecision
IP-15 Optical Parameters Imprecision (Page 3 of 3)
3
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 46
3
- Replace Diluent Container.
- From Main Menu, press
D IAGNOSTICS
F 12
F 1
SET POINT ENTRY
- Veri fy RBC/PLT Threshold is 550.
- Ensure RBC/PLT Mixing Chamber isbeing rinsed and drained properly.
- Verify 0oand 10oLog Amplifiers(VP-28).
- Verify 0oand 10oGain(VP-26),(VP-27).
- Replace Shear Valve Sect ions (PL-9.1).
- Replace MAM (PL-12).
- Replace SPM (PL-10).
- Replace CPU/DCM(PL-13).
5 7URXEOHVKRRWLQJ IP-16 HGB Imprecision (Page 1 of 1)
IP-16 HGB Imprecision (Page 1 of 1)
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300
August 19982 - 47
Start
- Clean Fan Filters and verify all Fans are
running properly.- Ensure there is at least 6" of clearance
behind and on the left side ofInstrument.
- Ensure Instrument is not subject todrastic temperature variations (direct
sunlight, heating/air conditioning ducts).
- If Fan Filters were clogged and thencleaned, al low a 15 minute temperature
stabilization period.
- Clean Shear Valve(VP-48).
- Manually clean HGB Flow Cell (VP-49).
- Verify System Precision.
- From Main Menu, press
RUN
SPECIMEN TYPE
- Select empty Q.C. File
Q.C. SPECIMEN
- Run one Patient 10 times.
- Review HGB CV%(VP-57).
HGB CVsOK
?
- Ensure HGB Flow Cel l is being
rinsed, drained, and ventedproperly. Verify Valves 51, 94, 93,(VP-10)and tubing . Replace asnecessary (PL-4),(TM-1) .
- Clean HGB Lyse Syr inge.
- Verify HGB Lyse Syringe (VP-6),
(VP-7). Replace as necessary(PL-7).
- Inspect and re-seat Cable at J18of FCM(PL-4.2).
- Replace HGB Flow Cel l (PL-4.2).
- Replace FCM(PL-14.1).
- Replace CPU/DCM(PL-13).
Total Call
YES
- Replace HGB Lyse
Container>
HGB CVsOK
?
Total Call
N O
YES
N O
N O
57URXEOHVKRRWLQJ IP-17 Primary Vacuum/Pressure Isolation,
IP-17 Primary Vacuum/Pressure Isolation, (Page 1 of 9)
Start
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300
August 1998 2 - 48
Start
- Reinitialize Analyzer
From Main Menu:
D IAGNOSTICS
MORE INITIALIZATION
- Remove Left Side Cover and
extend Air Supply(RR-7) toobserve pumps. (Vac-Front,Pres-Rear)
Both
ConstantlyRunning
?
4
Pres
Constant lyRunning
?
N O
1
VacConstant ly
Running
?
N O
N O
- Disconnect tubing frompositions 6 and 7 on AirSupply Manifold, and
observe Pumps and VPM
Pump LEDs. See Figures
5
6
- Observe LEDs. SeeFigure
YES
YES
YES
1
2
3
4
5
6
7
12PSI
9PSI
5PSI12"Hg8
"Hg
12PSI
12"Hg
TO
FLOW
PANEL
To S L
ToS L
32004
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IP-17 Primary Vacuum/Pressure Isolation, (Page 2 of 9)
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1
Both LEDsO N
?
- Replace Pump RelayBoard(PL-5)
N O
- Reinitialize Analyzer
From Main Menu:
D IAGNOSTICS
MORE
INITIALIZATION
- Observe Flow Panel
valves
YE S
All ValvesClose Then
Open
?
- Check +12V UR and Fuse.
See Figure- Replace if necessary(PL-1)
YE S
Prob lem
Corrected
?
Total Call
Y ES
- Replace Power Supply(PL-1)
N O
FuseBlown
?
Check fuse again
N O 8
2
N O
Y ES
+12 VNRFuse (Top)
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IP-17 Primary Vacuum/Pressure Isolation, (Page 3 of 9)
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2
LEDs BrightThen Dim When
Activated
?
- Whi le observing VPMSolenoid LEDs (SeeFigure) press:
INITIALIZATION
3
YES
- Check +24V UR (28.9V-37.7V) and Fuse
(Bottom Fuse)
- Replace Fuse ifnecessary (PL-1)
N O
Prob lem
Corrected
?
Total Call
YES
- Check Fuse again
FuseBlown
?
NO
- Replace Power Supply(PL-1)
N O 8
YES
Note: If the +24 UR is approximatelythe same as the +12 UR, the +24VFuse is blown or the +24V Power
Supply is defective.
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3
- Pinch tubing as shown inFigure
PumpsStop
?
- Inspect 12 psi and 12" Hgplumbing andAccumulators. Replaceas necessary(PL-5),
(TM-2)
- Inspect Vent Valves 1 and4. Replace as necessary(PL-5)
- Replace VPM (PL-5)
N O
- Unpinch tubing
- Pinch tubing at positions6 and 7 that supply 12psi and 12" Hg to SampleLoader
YES
PumpsStop
?
- Inspect Sample Loaderplumbing and 3-WayValves. Replace asnecessary (PL-18.3),
(TM-3)
YES- Ensure Valves 31 and 32,
97 and 98, 17 and 18 arenot open at the same
time.
- Inspect Flow Panel 12 psiand 12" Hg plumbing for
leaks.
N O
Pinch
1
2
3
4
5
6
12PSI
9PSI
5PSI12"Hg
8"Hg
12PSI12"H
TO
FLOW
PANEL
o S
To S L
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IP-17 Primary Vacuum/Pressure Isolation, (Page 5 of 9)
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- Observe Pressure PumpLED. See Figure Page 1
LEDON
?
- Replace Pump Relay
Board (PL-5)
N O
- Pinch tubing See Figurethis page
PumpStops
?
NO
- Inspect 12 psi plumbingand Accumulator.Replace as necessary(PL-5),(TM-2)
- Inspect Vent Valve 1.Replace as necessary(PL-5)
- Replace VPM(PL-5)
- Unpinch tubing and pinchat position 6 on Manifold.
YES
PumpStops
?
- Inspect Sample Loader
plumbing and 3-WayValves. Replace asnecessary (PL-18.3),(TM-3)
- Inspect Flow Panel 12 psiplumbing for leaks.
N O
YES
YES
Pinch
1
2
3
4
5
6
7
12PSI
9PSI
5P
SI12"Hg
8"Hg12PSI12"Hg
TO
FLOW
PANEL
ToS L
ToS L
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IP-17 Primary Vacuum/Pressure Isolation, (Page 6 of 9)
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 53
- Observe Vacuum PumpLED. See Figure Page 1
LEDON
?
- Replace Pump RelayBoard(PL-5)
NO
- Pinch tubing. See Figure
PumpStops
?
N O
- Inspect 12" Hg plumbingand Accumulator.Replace as necessary(PL-5), (TM-2)
- Inspect Vent Valve 4.Replace as necessary(PL-5)
- Replace VPM(PL-5)
- Unpinch tubing and pinchat position 7 on Manifold.
YES
PumpStops
?
- Inspect Sample Loaderplumbing and 3-Way
Valves. Replace asnecessary (PL-18.3),(TM-3)
- Inspect Flow Panel 12"Hg plumbing for leaks.
N O
YES
YES
Pinch
1
2
3
4
5
6
7
12PSI
9PSI
5PSI12"Hg
8"Hg
12PSI12"Hg
TO
FLOW
PANEL
ToS L
ToS L
32010
57URXEOHVKRRWLQJ
IP-17 Primary Vacuum/Pressure Isolation,
IP-17 Primary Vacuum/Pressure Isolation, (Page 7 of 9)
6- Replace VPM
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 54
NeitherPumpRuns
?
VAC
Doesn'tRun
?
Pres
Doesn'tRun
?
N O
N O
LEDsO N
?
- Reseat connectors VPM J18, PRMJ1, J2, J3, J4
- Check Fuse on PRM. Replace i f
necessary(PL-5)
- Veri fy 115VAC to PRM
- Replace PRM (PL-xx)
YES
Replace VPM(PL-5)
- Reseat connectors VPM J18, PRM
J1, J3- Check 115VAC across Pins 1 and 2
on PRM P3. IF present, replace VacPump. I f not, replace PRM(PL-5)
VAC LED
O N
?
YES
Pres LED
O N
?
YES
- Reseat connectors VPM J18,PRM J1, J2
- Check 115VAC across Pins 1 and2 on PRM P2. IF present, replace
Pres Pump. I f not, replace PRM(PL-5)
7
- Replace VPM(PL-5)
- Replace VPM(PL-5)
YES
YES
YES
N O
N O
N O
5 7URXEOHVKRRWLQJ IP-17 Primary Vacuum/Pressure Isolation,
IP-17 Primary Vacuum/Pressure Isolation, (Page 8 of 9)
7
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 55
- Re-connect tubing topositions 6 and 7 (6-Red,7-Green)
- Observe Vacuum andPressure Pumps andt ime the durat ion betweenrun cycles.
Pres
Cycles< 2 min
?
Va c
Cycles< 2 min
?
See Pressure Leaks(IP-18) See Vacuum Leaks (IP-19)
- Perform Vacuum andPressure LevelVeri f ication/Adjustment(VP-16)
- Perform Vacuum andPressure RecoveryVerification(VP-17)
BothOK
?
YES
- Replace Pump Head
- If problem persists,
replace Pump AssemblyPump Head:
Pressure (PL-5)
Vacuum (PL-5)
Pump Assy:
Pressure (PL-5)
Vacuum (PL-5)
YES
YES
Total Call
N O
N O
N O
5 7URXEOHVKRRWLQJ IP-17 Primary Vacuum/Pressure Isolation,
IP-17 Primary Vacuum/Pressure Isolation, (Page 9 of 9)
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- If the Fuse continues to blow,assemblies on the +12V UR or
+24V UR must be disconnectedone at a time until the shortedassembly is found andreplaced. (See Figures)
- Power-down instrument and
connect Meter between TP2-+12V or TP1-+24V and TP4 on
PSM and remove connectorsuntil short is found
- Replace defect ive assembly and
verify proper operation
CDM 2 & S DM/CDM Power Connec tors
J3 J1+24V UR
+24V UR
+12V UR
+12V UR
+24V UR
+24V UR
+24V UR
+12V UR
+12V UR
UR RE T
5
4
3
1
2
3
1
2
3
1
FromP.S.
J1/J2
ToS DM A
1
2
3
1
2
3
J2
ToS DM B
J4
ToS DM C
J5
ToS DM D
BackJ1
J2
J3
J4
UR RE T
UR RE T
UR RE T
1
345
13
12
4
123
5
RET
PS M
FC M
To
To
To
To
CDM2-J3FCM-J5
SDM/CDM-J3
Shear
ValveDriver-J3
SDM9-J10
Note: J4 and J5 are not onS DM /CDM
32011
5 7URXEOHVKRRWLQJ IP-18 Pressure Leak Isolation
IP-18 Pressure Leak Isolation (Page 1 of 7)
Note: This procedure is designed to isolate leaks in the pressure plumbing. Refer to IP-17 Primary Vacuum/Pressure Isolation for troubleshooting gross pressure failures.
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CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998 2 - 57
Start
- Reinitialize AnalyzerFrom Main Menu:
D IAGNOSTICS
MORE
INITIALIZATION
- Remove Left Side Cover andextend Air Supply (RR-7) toobserve Pres Pump. (Vac-Front, Pres-Rear)
- Observe Pressure Pumpand time the duration
between run cycles.
PumpCycles< 2 min
?
Total Call
N O
- Adjust Pressure Levels ifnecessary(VP-16)
1
YES
5 7URXEOHVKRRWLQJ IP-18 Pressure Leak Isolation
IP-18 Pressure Leak Isolation (Page 2 of 7)
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LEDs BrightThen Dim When
Activated
?
- Whi le observing