CBE 403aBiochemical Separation Processes

Course Notes

Course Instructor : Dr. A. Margaritis, Ph.D., F.C.I.C., P. Eng.

Professor of Biochemical Engineering

Fall 2007

The University of Western Ontario

Faculty of Engineering

Department of Chemical and Biochemical Engineering

Table of Contents1. Introduction to Biochemical Separation Processes

2. Filtration Processes

3. Cell Disruption Methods

4. Membrane Separation Processes

5. Centrifugation Processes

6. Chromatographic Processes and Size Exclusion Chromatography

7. Adsorption Processes

8. Ion Exchange Processes

9. Affinity Chromatography Methods

1. Introduction to Biochemical Separation Processes

Bioprocess flow sheet for penicillin production from P. chrysogenum

Product Concentration in Fermentation Broth vs. Product Selling price

Log10 bioproduct price vs. yearly production volume (World)

FERMENTATION

Process Integration

Intracellular vs. Extracellular Products

Product Isolation / Purification

General Downstream processing Considerations

Formulation of a Purification Process