case study 2 - tissue engineering scaffold
TRANSCRIPT
Developing a Tissue
Engineering Scaffold
Stephen FischerGabriella Shull
BME 313
The Challenge• Objective: Create a scaffold that can house the most amount of
cells• Stipulation: Must withstand 3D rotational and translational motion
• Description: Students are given an overview on tissue engineering and the project. Students split into small groups and are provided materials.
• Target Audience Age: Any age group• Background may be explained at more rudimentary level for younger students
• Duration of Project: ~30 min. for building and 15 - 30 min. for discussion
Project Protocol● Protocol can be customized
according to audience or resources.
● Total Duration ○ 37 - 70 minutes
● A short survey before and after may help gauge success of event.
Materials
Total: $33.66
*Note: These numbers accommodate many students. Materials are reusable as well.
Item Price Quantity Supplier
Popsicle Sticks $3.66 100/pack Walmart/ CVS
Pipe Cleaners $10.99 120/pack Walmart/ CVS
Straws $8.98 100/pack Walmart/ CVS
Glue $2.04 1 item Walmart/ CVS
Tape $3.50 1 roll Walmart/ CVS
Colored Cotton Balls $4.49 200/pack Walmart/ CVS
Some Examples
Skin Scaffold Biodegradable Scaffold
Flexible Scaffold
Competition (Optional)- Great way to foster creativity and collaboration as teams
- Two methods to accomplish this
1)Race to Builda) Build a scaffold within a timeframe that supports a designated number of cells
b) Contestants will go to judges for counting when they think they have achieved this
2)Design for Quantitya) Build a scaffold for a particular organ in a period of time that houses the most cells
b) The one with the most cells is the winner
Example: An
Introduction to Tissue
Engineering
Tissue Engineering Introduction
• Tissue Engineering is the creation of tissue to restore, replace, and maintain the function(s) of the body.
• Applications include replacing organs or pieces of organs that are damaged by disease, or injury.
• Can help save millions of livesSource: https://goo.gl/ASevvy
Tissue Engineering Components
1. Cellsa. basic unit of lifeb. require nutrients to stay alive
2. Scaffold (Extracellular Matrix)a. surrounds cells and helps them surviveb. similar to buildings and sidewalks
3. Chemical Signalsa. tells cells and scaffolds what to dob. similar to traffic lights, road signs, and telephone calls
http://www.intechopen.com/source/html/49155/media/image4.png
Scaffold Design
• Different types of scaffolds mimic different types of tissue
• Hard scaffold - bone, cartilage• Soft and Elastic - skin, most organs
• Major design limitations include:1) the porosity (or amount of voids in the scaffold)
- important for nutrient transport
2) the mechanical properties of the scaffold- different cells require
different mechanics
Source: http://www.cellsupports.com/
Source: http://goo.gl/1khB6u
Scaffold Design Challenge• https://goo.gl/HnHBFE
• Break up into teams of 2-3
• Obtain materials• pipe cleaners• Popsicle sticks• tape• cotton balls
• Go!!