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Implementation of computerized process and a management system at the
Leishmania Collection of the Oswaldo Cruz Institute [Coleção de Leishmania
do Instituto Oswaldo Cruz] – CLIOC
CARLOS HENRIQUE MARTINS DA SILVA1, SIDNEI DE SOUZA2, ELISA CUPOLILLO3
Laboratório de Pesquisa em Leishmaniose, Coleção de Leishmania do Instituto Oswaldo Cruz
IOC/FIOCRUZ, Instituto Oswaldo Cruz/Fundação Oswaldo Cruz, Av. Brasil 4365, Manguinhos, Rio de Janeiro, RJ, Brasil 2Centro de Referência em
Informação Ambiental - CRIA
CLIOC was created in 1980 with the support of the Oswaldo Cruz Institute, the Oswaldo Cruz Foundation and the World Health Organization.
CLIOC is registered with the World Federation for Culture Collections (WFCC- WDCM 731).
CLIOC is a partner of the demonstration project for a Global Biological Resource Centre Network.
The ABC of BRCs:
Authentication process for biological material
Best practice in preservation
Confirmed, validated information
From culture collection to BRC
Here we describe the computerization process adopted at CLIOC
by using a management system
CLIOC received more than 3,000 samples for deposit and/or characterization
and today has more than 1,000 strains available, representing two sections, three
subgenera and about 30 species.
Crithidia Leptomonas Herpetomonas Blastocrithidia Leishmania Trypanosoma Phytomonas Endotrypanum
Tripanossomatidae
Protozoa
Kinetoplastida
LeishmaniaViannia
Subreino
Ordem
Família
Gênero
Seções
Subgênero
Complexo
Espécie
L. donovani L. tropica L. mexicana L. major L. braziliensis L. guyanensis
L. donovani¹
L. archibaldi¹
L. infantum²
L. chagasi²
L. tropica
L. killicki
L. aetthiopica
L. major8
L. gerbilli8
L. enrietti
L. venezuelensis
L. forattinii **
L. amazonensis³
L. garnhami³
L. mexicana4
L. pifanoi4
L. aristidesi
L. braziliensis5
L. peruviana5
L. naiffiL. guyanensis6,7
L. panamensis6
L. shawi7
L. lainsoni
L. equatoriensis
L. colombiensis
L. hertigi
L. deanei
L. herrem
EuleishmaniaParaleishmania
L. arabica L. turanica L. lindenbergi L. utingensis
Sauroleishmania*
L. agami
L. adleri
L. tarentolae
L.hemidactyli
L. ceramodactyli
L. zmeevi
L. hoogstraali
L. gymnodactly
CLIOC is now fully computerized through
this system, and is able to register a wealth
of information about the samples collected,
including biological (e.g. growth curve, in
vitro sensibility to antimonial, infectivity,
virulence), biochemical (e.g. isoenzymatic
pattern, proteomic), molecular (e.g. DNA
sequences, gene expression), clinical and
ecological traits.
Services through online forms and the online
catalog are available through its web site
(http://clioc.ioc.fiocruz.br).
Stock control and strain distribution
management are also covered by
Sicol.
CLIOC is also partially
georeferenced and connected
to the speciesLink network,
aiming to help predictive
studies on Leishmania species
and genotype dispersion.
These strains came from many localities and different hosts, most from
South America.
A constant concern of this collection has been with
epidemiological data associated with the strains collected.
All deposited strains are submitted to
biochemical identification. Biological and
molecular characterizations have also been
conducted through research projects, lead
by CLIOC or by other research groups.
Supported by
Records from the Country of Origin
Brazil - 2303 Algeria - 1 Argentina - 23
Azerbaijian - 2 Belize - 6 Bolivia - 71
China - 8 Colombia - 36 Costa Rica - 35
Egypt - 1 Ecuador - 111 Ethiopia - 6
France - 3 Greece - 2 Honduras - 18
India - 5 Iraq - 2 Israel - 16
Jordan - 3 Kenia - 3 Mexico - 14
Nicaragua - 17 not informed - 18 Panama - 21
Paraguay - 37 Peru - 16 Portugal - 4
Dominican Republic - 2 Senegal - 1 Sudan - 23
Sw eden - 1 Tunísia - 3 Turkmenistan - 1
USSR - 11 Uzbekistan - 1 Venezuela - 241