NAME: Brenna GagliardiCOURSE: BIOL/BIOC 2200 A

MOCK EXAM (FOR PRACTICE ONLY)

It is most beneficial to you to write this mock midterm UNDER EXAM CONDITIONS. This means: • Complete the midterm in __ hour. • Work on your own and attempt every question. • Keep your notes and textbook closed.

After the time limit, go back over your work with a different colour or on a separate piece of paper and try to do the questions you are unsure of. Record your ideas in the margins to remind yourself of what you were thinking when you take it up at PASS.

The purpose of this mock exam is to give you practice answering questions in a timed setting and to help you to gauge which aspects of the course content you know well and which are in need of further development and review. Use this mock exam as a learning tool in preparing for the actual exam.

Please note:

Complete the mock exam before attending the take-up session. During the session you can work with other students to review your work.

Often, there is not enough time to review the entire exam in the PASS workshop. Decide which questions you most want to review – the Facilitator may ask students to vote on which questions they want to discuss in detail.

Facilitators will not distribute an answer key for mock exams . The Facilitator’s role is to help students work together to compare and assess the answers they have. If you are not able to attend the PASS workshop, you can work alone or with others in the class.

PASS worksheets are designed as a study aid only for use in PASS workshops. Worksheets may contain errors, intentional or otherwise. It is up to the student to verify the information contained within by attending the PASS workshop.

Good Luck writing the Mock Exam!!

Take-up Session #1: Thursday, October 8th, 5:35-6:55 PMTake-up Session #2: Friday, October 9th, 11:35-12:55 PMOffice Hour: Tuesday, October 13th, 12:35-2:35 PMZoom Meeting Information:I will be emailing the Zoom meeting details (i.e. Zoom link, Meeting ID & Password) but it can also be found on your course’s CULearn page.Contact Information: brennagagliardi@cmail.carleton.ca

NAME: Brenna GagliardiCOURSE: BIOL/BIOC 2200 A

MOCK EXAM (FOR PRACTICE ONLY)

1. Which of the following is not a function of carbohydrates?a. Store fuel/energy in the form of starch or glycogen.b. Act as precursors in hormone synthesis.c. Provide energy through metabolic pathway.d. Supply carbon for synthesis of other compounds.e. Form structural components in cells and tissues.

2. Enzymes _________ reaction rates by _________ activation energy. They do this by coupling otherwise __________ reactions.

a. Decelerate, decreasing, favourableb. Accelerate, increasing, unfavourable.c. Accelerate, increasing, favourable.d. Decelerate, decreasing, favourable.e. Accelerate, decreasing, unfavourable.

3. Carbon is the basic building block of biomolecules because…a. Carbon can form an almost infinite number of compounds as a result of its

capacity to make as many as 5 bonds highly stable bonds.b. It can form single, double and triple bonds.c. Carbons can be tetrahedral, trigonal and linear.d. All of the abovee. B and C f. A and B

4. Below a pKa of 2.20 most amino acids have a…a. Ionized amino groupb. Protonated carboxylic acid groupc. Ionized carboxylic acid groupd. Protonated amino groupe. A and Bf. C and D

5. Which of the following is incorrect about Histidine?a. Its R-group is a weak acidb. It is often found in active sitesc. It is hydrophobicd. It can bind metal ions such as zinc, nickel and copper.e. A and C

NAME: Brenna GagliardiCOURSE: BIOL/BIOC 2200 A

MOCK EXAM (FOR PRACTICE ONLY)

6. Which of the following is not a protein?a. Hormonesb. Enzymesc. Hemoglobind. DNAe. Antibodies

7. What is the strongest bond inside of a protein?a. Disulfide bridgesb. Hydrophobic interactionsc. Hydrogen bondingd. Salt bridges

8. The quantity of ____________ determine the flexibility of the resulting fibrous protein?

a. Hydrophobic Interactionsb. Peptide Bondsc. Hydrogen Bondsd. Disulfide Bridgese. All of the above

9. Given the chemical equation: 2 A+3B⇌C+4D. What is the appropriate representation of K eq?

a. K eq=[A ]3 [B ]2

[C ]4[D ]

b. K eq=[C ]2 [D ]3

[A ] [B ]4

c. K eq=[C ] [D ]4

[A ]2 [B ]3

d. K eq=[A ]2 [B ]3

[C ] [D ]4

e. None of the above10.Which of the following statements is incorrect about thermodynamics?

a. Thermodynamics describes the relationship among various form of energy.b. Thermodynamics help scientists to understand how metabolic processes are

designed.c. Thermodynamics can be used to determine if a process is possible.d. Thermodynamics can be used to determine the rate at which processes will

occur.

NAME: Brenna GagliardiCOURSE: BIOL/BIOC 2200 A

MOCK EXAM (FOR PRACTICE ONLY)

e. All are correct.

NAME: Brenna GagliardiCOURSE: BIOL/BIOC 2200 A

MOCK EXAM (FOR PRACTICE ONLY)

11. If ∆G°>0 then the reaction is…?a. Spontaneous; Exergonicb. Spontaneous; Endergonicc. Non-Spontaneous; Exergonicd. Non-Spontaneous; Endergonic

12. According to LeChatelier’s Principle if more reactant is added to a solution then the reaction will proceed…?

a. To the leftb. To the rightc. No changed. Depends on the reaction

13.Which of the following statements is incorrect about mass action?a. For a reaction to occur, two or more molecules must collide.b. The orientation of the molecules does not affect the success of a collision.c. The collision must be of sufficient energy to break the necessary.d. All of the abovee. None of the above

14. In an alpha-keratin microfibril how many individual stranded molecules are present?a. 16b. 32c. 48d. 8e. None of the above

15.α-helices fit into the _______ groove of DNA while β-sheets fit well into the _______ groove of DNA.

a. Major, Majorb. Major, Minorc. Minor, Minord. Minor, Major

16.What is the correct order of the solid-phase peptide synthesis method?a. Activation, coupling, deprotectionb. Deprotection, activation, couplingc. Coupling, deprotection, activationd. Deprotection, coupling, activation

NAME: Brenna GagliardiCOURSE: BIOL/BIOC 2200 A

MOCK EXAM (FOR PRACTICE ONLY)

17.Which of the following is incorrect about Proline?a. It is hydrophilic.b. Its side chain forms a ring.c. It is not actually an amino acid. d. It typically forms turns.e. A and C

18.When an amino acid is in an acidic solutiona. The carboxylic acid ionizes and the pH of the solution increases.b. The carboxylic acid ionizes and the pH of the solution increases.c. The carboxylic acid ionizes and the pH of the solution decreases.d. The amino group is protonated and the pH of the solution increases.e. The amino group is ionizes and the pH of the solution decreases.

19.The Henderson Hasselbalch Equation is…a. pK❑a=pH+log ¿¿

b. pH=pK❑a+log ¿¿

c. pH=pK❑a+log ¿¿

d. p Ka=pH+ log¿¿

e. pH=p Ka+ ln¿¿20.Which of the following are considered essential ions?

a. Mn, Co, Fe, Cu, Znb. Ca, K, Mg, Na, Clc. C, H, N, O, P, Sd. B, F, Al, Si, Ni, Ie. A and D

21.What is the difference between saturated and unsaturated fatty acids?a. Saturated fatty acids contain some double carbon-carbon bond while

unsaturated fatty acids contain only single carbon-hydrogen bonds.b. Saturated fatty acids are composed of more hydrogen atoms than

unsaturated fatty acids.c. Saturated fatty acids contain only single carbon-carbon bonds while

unsaturated fatty acids contain some carbon-carbon double bonds.d. B and Ce. A and C

NAME: Brenna GagliardiCOURSE: BIOL/BIOC 2200 A

MOCK EXAM (FOR PRACTICE ONLY)

22.Which of the following does not influence electronegativity?a. The number of electrons in an atom

b. The distance from the nucleusc. The number of protons in the nucleusd. The amount of screening by inner electronse. The number of neutrons in the nucleus

23.The following image illustrates…

Figure 1 H. Robert Horton, et al (2012). Principles of Biochemistry 5th Edition. Pearson Education Inc.

a. carboxylateb. carbonyl c. ketone d. carboxylic acid e. aldehyde

24.Above a pKa of 9.52 most amino acids have a…a. Ionized amino groupb. Protonated carboxylic acid groupc. Ionized carboxylic acid groupd. Protonated amino groupe. A and Bf. C and D

25.Which of the following is NOT an essential amino acid?a. Arginineb. Lysinec. Cysteined. Methioninee. Valine

26.Hydrogen bonding between the backbone of amino acids in a polypeptide chain are characteristic of:

a. Primary protein structureb. Secondary protein structurec. Tertiary protein structured. Quaternary protein structure

NAME: Brenna GagliardiCOURSE: BIOL/BIOC 2200 A

MOCK EXAM (FOR PRACTICE ONLY)

27.Heat causes the denaturation of globular proteins by influencing the…?

a. Weak side-chain interactionsb. Hydrophobic interactionsc. Hydrogen bondsd. Disulfide bridges

28.Levinthal’s Paradox illustrates that proteins must…?a. only sample through limited conformationsb. fold by specific pathwaysc. sample all possible conformations during foldingd. A and Be. All of the above

29.Using the following image what is the correct matching of terms?

Figure 2 H. Robert Horton, et al (2012). Principles of Biochemistry 5th Edition. Pearson Education Inc.

a. (A) Amino Group, (B) Carboxyl Group, (C) R-Groupb. (A) Carboxyl Group, (B) Amino Group, (C) R-Groupc. (A) Carboxyl Group, (B) R-Group, (C) Amino Groupd. None of the above

30. If ∆G°<0 then the reaction is…?a. Spontaneous; Exergonicb. Spontaneous; Endergonicc. Non-Spontaneous; Exergonicd. Non-Spontaneous; Endergonic

31.Which of the following statements is incorrect about the equilibrium constant?a. K eq≫1 then more products are present than reactants.b. K eq≪1 then more reactants are present than productsc. K eq≫1 then more reactants are present than productsd. A and Be. C and B

C

B

A

NAME: Brenna GagliardiCOURSE: BIOL/BIOC 2200 A

MOCK EXAM (FOR PRACTICE ONLY)

32.Protein X is found to insoluble in water, mechanically strong and flexible. This is most likely a…?

a. Globular Proteinb. Fibrous Proteinc. Membrane Proteind. Motor Protein

33.Which of the following amino acids is an α-helix breaker?a. Glycineb. Lysinec. Prolined. Cysteinee. A and Cf. A and B

34.Solid-phase peptide synthesis involves the successive _______ of amino acids to create a linear peptide chain. The ________ of the peptide is covalently bound to a solid support or resin.

a. Deletion, C-Terminusb. Addition, N-Terminusc. Deletion, N-Terminusd. Addition, C-Terminus

35.Which of the following is incorrect about Cysteine?a. It forms disulfide crosslinksb. It typically forms α-helices c. It can bind metals such as zinc and copperd. It is more acidic that most amino acidse. B and D

36.The effectiveness of a buff is determined by:a. The pH of the solution; buffers work best within 3 pH units above and below

the pKa.b. The pH of the solution; buffers work best within 1 pH unit above and below

the pKa.c. The concentration of the buffer; the more present, the more the buffering

capacity.d. A and Ce. B and C

NAME: Brenna GagliardiCOURSE: BIOL/BIOC 2200 A

MOCK EXAM (FOR PRACTICE ONLY)

37.Living cells are ________. They are in a steady state, meaning a constant flow of ________, prevents them from becoming ________.

a. Stable, Water, Disorganizedb. Unstable, Ions, Organizedc. Unstable, Energy, Disorganized d. Stable, Energy, Organizede. Unstable, Ions, Disorganized

38.An ester combines with water to produce a carboxylic acid and an alcohol. This is an example of a:

a. Isomerization Reaction b. Oxidation-Reduction Reactionc. Hydrolysis Reaction d. Addition Reactione. Nucleophilic Substitution Reaction

39.Which of the following statements are NOT correct about amino acid stereochemistry:

a. All amino acids have a chiral α-carbon. (glycine does not)b. Threonine and Isoleucine have 2 chiral carbons each.c. Amino acids used in proteins are in the D-conformation.d. A and Ce. B and C

40.Which of the following is incorrect about Peptide Bonds?a. It has a double bond character.b. It has a planar structure.c. Most peptide bonds have a cis-configuration.d. The hydrolysis of a peptide bond is thermodynamically favored.

41.Hydrophobic interactions are characteristic of:a. Primary protein structureb. Secondary protein structurec. Tertiary protein structured. Quaternary protein structure

42.A change in pH can denature a protein by influencing the…?a. Hydrogen bondsb. Disulfide bridgesc. Weak side-chain interactiond. Hydrophobic interactions

NAME: Brenna GagliardiCOURSE: BIOL/BIOC 2200 A

MOCK EXAM (FOR PRACTICE ONLY)

43.Molten globules are formed because of…?a. Hydrophobic Interactionsb. Peptide Bondsc. Hydrogen Bondsd. Disulfide Bridgese. All of the above

44. In affinity chromatography proteins migrate down the column as a function of…?a. negative chargeb. molecular massc. binding affinity to a ligandd. positive charge

45.What was the primary conclusion of Anfinsen Experiment?a. All the information necessary for folding the peptide chain into its native

structure is contained in the secondary structure of the protein.b. All the information necessary for folding the peptide chain into its native

structure is contained in the DNA that encodes the protein.c. All the information necessary for folding the peptide chain into its native

structure is contained in the primary structure of the protein.d. All the information necessary for folding the peptide chain into its native

structure is contained in the RNA that encodes the protein.46.Alpha-helices containing ______ amino acids on either end and ______ amino acids

in the middle can be used to span membranes.a. Hydrophobic, Hydrophilicb. Hydrophilic, Hydrophilicc. Hydrophilic, Hydrophobicd. Hydrophobic, Hydrophobic

47._________ contains a high-energy bond that is used to drive the ________ of a peptide bond.

a. Aminoacyl-DNA’s; Hydrolysisb. Aminoacyl-RNA’s; Formationc. Aminoacyls-DNA’s; Formationd. Aminoacyl-RNA’s; Hydrolysis

NAME: Brenna GagliardiCOURSE: BIOL/BIOC 2200 A

MOCK EXAM (FOR PRACTICE ONLY)

48.The Isoelectric point of an amino acid is the ____ at which the majority of its molecules in solution have _____________.

a. pKa, no net chargeb. pH, no net chargec. pH, positive charged. pKa, negative chargee. pH, negative charge

49. In size exclusion chromatography proteins migrate down the column as a function of…?

a. negative chargeb. molecular massc. binding affinity to a ligandd. positive charge

50.When an amino acid is in a basic solution…a. The carboxylic acid ionizes and the pH of the solution increases.b. The carboxylic acid ionizes and the pH of the solution decreases.c. The amino group is protonated and the pH of the solution increases.d. The amino group is ionizes and the pH of the solution decreases.e. The carboxylic acid is protonated and the pH of the solution increases.

NAME: Brenna GagliardiCOURSE: BIOL/BIOC 2200 A

MOCK EXAM (FOR PRACTICE ONLY)

BONUS QUESTIONSUse the following diagram of a polypeptide to answer the next 2 questions.

Figure 3. https://www.chegg.com/homework-help/questions-and-answers/polypeptide-shown-circle-peptide-bonds-polypeptide-amino-acid-indicate-polar-non-polar-ele-q29958159

51.The third amino acid residue from the N-terminus is…a. Alanineb. Glycinec. Threonined. Valinee. None of the above

52.The fourth amino acid residue from the N-terminus is…a. Aspartic Acidb. Glutaminec. Asparagined. Glutamic Acide. None of the above