carl henrik gørbitz, ute krengel department of chemistry, uio
DESCRIPTION
Biological chemistry. Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO. Crystal engineering. Biomarker identification. Protein crystallography. Molecules. H 2. ethane. sucrose. Molecules. Molecules. Crystal. Crystal. ”Crystal engineering”, we make a supermolecule!. - PowerPoint PPT PresentationTRANSCRIPT
![Page 1: Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO](https://reader036.vdocuments.us/reader036/viewer/2022062305/56814986550346895db6cf2f/html5/thumbnails/1.jpg)
Carl Henrik Gørbitz, Ute KrengelDepartment of Chemistry, UiO
![Page 2: Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO](https://reader036.vdocuments.us/reader036/viewer/2022062305/56814986550346895db6cf2f/html5/thumbnails/2.jpg)
Crystal engineering
Protein crystallography
Biomarker identification
![Page 3: Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO](https://reader036.vdocuments.us/reader036/viewer/2022062305/56814986550346895db6cf2f/html5/thumbnails/3.jpg)
Molecules
H2
ethanesucrose
![Page 4: Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO](https://reader036.vdocuments.us/reader036/viewer/2022062305/56814986550346895db6cf2f/html5/thumbnails/4.jpg)
Molecules
![Page 5: Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO](https://reader036.vdocuments.us/reader036/viewer/2022062305/56814986550346895db6cf2f/html5/thumbnails/5.jpg)
Molecules
![Page 6: Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO](https://reader036.vdocuments.us/reader036/viewer/2022062305/56814986550346895db6cf2f/html5/thumbnails/6.jpg)
Crystal
![Page 7: Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO](https://reader036.vdocuments.us/reader036/viewer/2022062305/56814986550346895db6cf2f/html5/thumbnails/7.jpg)
Crystal
”Crystal engineering”, we make a supermolecule!
![Page 8: Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO](https://reader036.vdocuments.us/reader036/viewer/2022062305/56814986550346895db6cf2f/html5/thumbnails/8.jpg)
Building blocks
nodelinker
![Page 9: Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO](https://reader036.vdocuments.us/reader036/viewer/2022062305/56814986550346895db6cf2f/html5/thumbnails/9.jpg)
Problem 1
Molecules are very small, hard to
put together one by one
Xe-atoms on Ni-surface (Don Eigler, 1989)
![Page 10: Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO](https://reader036.vdocuments.us/reader036/viewer/2022062305/56814986550346895db6cf2f/html5/thumbnails/10.jpg)
Problem 2
As they are so small, we must put together very many before we reach a macroscopic size
A 1 x 1 x 1 mm sugar crystal contains 1,4 · 1018 molecules
![Page 11: Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO](https://reader036.vdocuments.us/reader036/viewer/2022062305/56814986550346895db6cf2f/html5/thumbnails/11.jpg)
Building blocks
![Page 12: Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO](https://reader036.vdocuments.us/reader036/viewer/2022062305/56814986550346895db6cf2f/html5/thumbnails/12.jpg)
Building blocks
![Page 13: Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO](https://reader036.vdocuments.us/reader036/viewer/2022062305/56814986550346895db6cf2f/html5/thumbnails/13.jpg)
Glue
![Page 14: Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO](https://reader036.vdocuments.us/reader036/viewer/2022062305/56814986550346895db6cf2f/html5/thumbnails/14.jpg)
Intermolecular forces
Must be: strong Directional
Two main types used in CE: Hydrogen bonds Metal coordination
![Page 15: Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO](https://reader036.vdocuments.us/reader036/viewer/2022062305/56814986550346895db6cf2f/html5/thumbnails/15.jpg)
MOFs
“Metal-Organic Frameworks (MOFs) are crystalline compounds consisting of metal ions or clusters coordinated to often rigid organic molecules to form one-, two-, or three-dimensional structures that can be porous” (from Wikipedia)
MOF-5
![Page 16: Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO](https://reader036.vdocuments.us/reader036/viewer/2022062305/56814986550346895db6cf2f/html5/thumbnails/16.jpg)
Can we do this without metal ions?
![Page 17: Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO](https://reader036.vdocuments.us/reader036/viewer/2022062305/56814986550346895db6cf2f/html5/thumbnails/17.jpg)
Guanidinium derivatives
Fumaric acid complex
CN
N
NH
H
H
H
H
H
+
![Page 18: Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO](https://reader036.vdocuments.us/reader036/viewer/2022062305/56814986550346895db6cf2f/html5/thumbnails/18.jpg)
Guanidinium derivatives
![Page 19: Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO](https://reader036.vdocuments.us/reader036/viewer/2022062305/56814986550346895db6cf2f/html5/thumbnails/19.jpg)
DNA-based systems
![Page 20: Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO](https://reader036.vdocuments.us/reader036/viewer/2022062305/56814986550346895db6cf2f/html5/thumbnails/20.jpg)
DNA-based systems
![Page 21: Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO](https://reader036.vdocuments.us/reader036/viewer/2022062305/56814986550346895db6cf2f/html5/thumbnails/21.jpg)
DNA-based systems
![Page 22: Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO](https://reader036.vdocuments.us/reader036/viewer/2022062305/56814986550346895db6cf2f/html5/thumbnails/22.jpg)
Applications
Construction of polar materials for non-linear optics molecular magnets porous materials for storage molecular sieves sensors molecule traps biological model systems catalysts and much more
![Page 23: Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO](https://reader036.vdocuments.us/reader036/viewer/2022062305/56814986550346895db6cf2f/html5/thumbnails/23.jpg)
Biomarker identification
Biomarker: A biological molecule found in blood, other body fluids, or tissues that is a sign of a normal or abnormal process, or of a condition or disease. A biomarker may be used to see how well the body responds to a treatment for a disease or condition. Also called molecular marker and signature molecule
Present in small amounts = hard to identify
![Page 24: Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO](https://reader036.vdocuments.us/reader036/viewer/2022062305/56814986550346895db6cf2f/html5/thumbnails/24.jpg)
Biomarker identification
Nano-HPLC-system
![Page 25: Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO](https://reader036.vdocuments.us/reader036/viewer/2022062305/56814986550346895db6cf2f/html5/thumbnails/25.jpg)
Group
Carl Henrik
Fassil
Lianglin
Malgorzata
Lise-Lotte
StevenOla
![Page 26: Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO](https://reader036.vdocuments.us/reader036/viewer/2022062305/56814986550346895db6cf2f/html5/thumbnails/26.jpg)
Protein crystallography
![Page 27: Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO](https://reader036.vdocuments.us/reader036/viewer/2022062305/56814986550346895db6cf2f/html5/thumbnails/27.jpg)
Structure/Function Studies of Structure/Function Studies of Medically Relevant SystemsMedically Relevant Systems
Department of Chemistry
Ute Krengel
University of Oslo
![Page 28: Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO](https://reader036.vdocuments.us/reader036/viewer/2022062305/56814986550346895db6cf2f/html5/thumbnails/28.jpg)
X-ray crystallography
Complementary techniques (molecular biology, protein chemistry, ligand binding studies, cell biology, molecular docking, organic chemistry)
MethodsMethods
![Page 29: Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO](https://reader036.vdocuments.us/reader036/viewer/2022062305/56814986550346895db6cf2f/html5/thumbnails/29.jpg)
Glycobiological Targets◦Mucins◦Bacterial Toxins◦Bacterial Adhesins◦Anti-tumor Antibodies◦Mushroom Lectins
Enzymes◦Chorismate mutases
GlycoNor
TargetsTargetsProtStru
ct
![Page 30: Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO](https://reader036.vdocuments.us/reader036/viewer/2022062305/56814986550346895db6cf2f/html5/thumbnails/30.jpg)
Glycobiological Targets:◦Bacterial Toxins – Structure and Function
Investigation of blood group dependence Delivery mechanisms? Drug design
◦Anti-tumor Antibodies for Immunotherapy Recombinant production, crystallization and X-ray
structure determination
Masters ProjectsMasters Projects
![Page 31: Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO](https://reader036.vdocuments.us/reader036/viewer/2022062305/56814986550346895db6cf2f/html5/thumbnails/31.jpg)
GroupGroup
Ute
Øyvind
Hedda
DanielJulie
Dipankar
Dani