carinata summit 2016 update on breeding efforts in carinata€¦ · • generating seed for two...
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Carinata Summit 2016
Update on Breeding Efforts in Carinata
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Growth of Agrisoma & Collaborator R&D teams
Agrisoma R&D Team Carinata “on the ground” R&D Teams
Management- J. Klingenberg (VP Product Development)- M. Lindenbaum (VP Technology)- D. Males (Director Breeding & Agronomy)- M. Lortie (Director Regulatory)
Saskatoon labs- R. Bennett (Breeder)- S. Rooke (Asst. breeder)- M. Moore (DH lead)- K. Czechowicz (DH group)- V. Catinot (DH group)- T. Fowler (DH group)- K. Livingstone (DH group)- E. Hill (Crossing specialist)- P. Fu (Genetic diversity)- E. Risseeuw (Molecular)- L. Chen (Molecular)- T. Hoffman (Analytics)
Greenhouse- M. Troesch (GH Lead)- S. Klashinsky (GH)
Field team- C. Bliss (Agronomist)- M. Henke (Agronomist)- K. Wilson (Agronomist)- R. Attri (Nursery Manager)
INIA UruguayLizama Farms ChileUniversity of FloridaAuburn UniversitySouth Dakota State UniversityNorth Dakota State UniversityMontana State UniversityUnited States Department of Agriculture (various extensions)Agriculture and Agri-Food CanadaNational Research Council of CanadaAgri-ARM Groups (Western Canada)Westwind Ag ResearchFarming SmarterMustard 21+ Other groups involved in testing of oil, meal, processing, etc.
R&D efforts enhanced through collaborative effort and development of local expertise in each geography
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Progress in experimental line production
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No. of DH lines field evaluated
Project Total no. plantlets
analyzed
No. doubled
(2N)
Ratio fertile
Cross set #1 10,341 5,443 52.6%
Cross set #2 4,138 2,227 53.8%
Cross set #3 10,157 3,205 31.6%
Totals for 2015 24,636 10,875 44.1%
Target: Consistently produce 8-10K DH lines per year
Simultaneous, efficiency increased by removing lines at early stage using:
i) Molecular markers (beginning to use first set of markers)
ii) Flow cytometer (removes haploid plants at plantlet stage)
DH team effort: Produce large number lines on front end to reach goals, increase chance of successMolecular team effort: Marker discovery phase, implement markers in high throughput system
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Progress in Agronomic Potential of Experimental lines
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5 or less 6 7 8 9
2014 Summer 2015 Summer 2015-16 Winter
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5 or less 6 7 8 9
2014 Summer 2015 Summer 2015-16 Winter
No
. o
f lin
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uat
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Agronomic Impression Rating; Higher is better, Checks = ~7
DH linesInbred selections
Cycle Percent of total rated 7 or better
2014 Summer 25%
2015 Summer 40%
2015-16 Winter 87%
Cycle Percent of total rated 7 or better
2014 Summer 7%
2015 Summer 31%
2015-16 Winter 83%
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Progress in Test Hybrid production
No. of A-line selections
Estimated geneticdistance from A120
13 0.400 – 0.450
18 0.350 – 0.400
2 0.300 – 0.350
0 0.250 – 0.300
0 0.200 – 0.250
2 0.150 – 0.200
1 <0.150
12 Data not available
Average all selections
0.392
• 269 unlinked SNP loci used in analysis, representing all chromosomes• Higher fraction indicates greater dissimilarity
Current: • Generating seed for two R-lines and twenty A-lines for test
hybrid production
Summer 2016: • Initiate production of test hybrid seed• Target: 20 A-lines x 2 R-lines = Seed for 40 test hybrids
produced
Winter 2016-17:• Target: 20 additional A-lines x 2 R-lines = Seed for 40
additional test hybrids produced
Summer 2017:• First set of 80 test hybrids in yield trials
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Progress in marker discovery: NAM project
Phenotyping beginning soon:
2016 N. Tier “test run”• 51 Founder Lines
2016-17 S. Tier• Inbred population
phenotyping (2,500 lines
Commercially relevant traits:
Includes traits such as• Early vigour• Flowering, maturity• Branching• Thousand seed weight• Seed quality parameters• Etc. etc.
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“Southern Tier” product development:
SE United States and Uruguay
Three high priorities, besides yield per se:1. Days to maturity2. Frost tolerance3. Very good shatter tolerance
Uruguay Testing 2015 - La Estanzuela, Uruguay
Entry Days to maturity (vs.
A110)
Shatter loss estimates
(kg ha-1) (% of yield)
Yield estimate (kg ha-1)
(% vs. A110)
159-4A1D 175 (Even) 69 (1%) 6155 (126%)
044-312D 170 (-5 days) 45 (1%) 5680 (116%)
044-3B1 175 (Even) 231 (4%) 5371 (110%)
159-1N1A 175 (Even) 67 (1%) 5309 (109%)
02.023 169 (-6 days) 48 (1%) 5039 (103%)
945-Z.063 176 (+1 day) 174 (4%) 4900 (100%)
A110 175 168 (3%) 4891 (100%)
044-3111 177 (+2 days) 355 (8%) 4555 (93%)
40.168 176 (+1 day) 272 (7%) 3806 (78%)
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“Southern Tier” product development:
Early maturity nursery
Cross Parental DTM vs. A110 in N. Tier
No. lines
#137 -1 x -5 28
#145 -7 x -3 237
#157 -5 x -1 522
• Earliest flowering 10% of lines from each population selected and self-pollinated
• Goal for 2015-16: Identify line with ≥ 7 days earlier maturity than A120
• Future crosses using parents evaluated in short day, winter environment (vs. long day, summer in current nursery)
Early maturity nursery, Feb 22, 2016
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“Southern Tier” product development:
Freeze tolerance
Name Rating - Site 1 Rating - Site 2
868-22B 40 20
25.102 50 30
439-A1 50 30
859-1 50 20
349-M22B 50 30
207-112 50 40
044-3B1 60 30
949-BI 60 20
044-3111 60 40
044-312D 70 40
159-11A2 70 50
AAC A120 70 50
AAC A110 70 40
044-3A21 80 40
400-A13C 80 50
40.063 80 40
19.308 80 60
137-5.106 90 40
More frost damage Less affected
• Rating indicates estimate of % plot damaged, three weeks after hard frost
• Green indicates entries that were better than checks in FL 2013-14 trials
Frost tolerance ratings: Quincy 2014-15
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“Southern Tier” product development:
Line ‘312D’
Isolation field “M-04”, Chile 2015-16
Rogueing Crew, Isolation field “HP-15”
Seed harvested from Isolation field “M-01”
• High yield potential variety in Florida and Uruguay testing
• Six isolation fields of top selections in Chile, rogueing and colour sorting for more uniform type
• Corresponding selections in 2015-16 FL yield trials
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“Southern Tier” product development:
Line ‘312D’
Isolation tent “HP-06”, Chile 2015-16
Trial % seed yield of current check variety
2014-15 Quincy, FL (No Cadre residue) 123%
2014-15 Quincy, FL (Cadre residue) 109%
2015 Uruguay yield trial 116%
2015 Uruguay strip trials (avg. 5 sites) 113%
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Future direction: Breeding efforts
Expand germplasm screening in Florida winter nursery: 2015-16 = 3 crosses, 800 lines screened for early maturity; 2016-17 = Two frost tolerant cross populations generated thus far
Expand validation of germplasm in Uruguay yield testing: 2015 = 10 entries x 1 site; looking at an additional site in 2016?
Numbers of DH lines produced for field testing will continue to increase
Identification and use of molecular markers continuing to become more important part of program, efficient selection of lines going into field testing
Collaboration among Agrisoma and many partners in a number of geographies makes the breeding program stronger; “eyes” on the ground and local expertise