capacity building for faba bean improvement · [email protected] . challenges for egyptian...
TRANSCRIPT
Challenges for Egyptian agriculture • < 3.5 % land suitable for agriculture, much of that newly
reclaimed • Population ~80 million; 109 million expected by 2030 • Renewable water resources (RWR):
• 779 m3/capita/year in 2004 • 534 m3/capita/year in 2030 • 1000 m3/capita/year: globally agreed water scarcity level
Modified from P. Rengasamy & P. Langridge
Factors limiting agricultural productivity
0.25 – 0.50 cm
Wind
Heat UV
Drought,
Leaf pathogens
Vision • Faba bean (Vicea faba): key protein crop for humans • New cultivation areas irrigated with brackish water • Tolerance to salt requires use of genetic resources in breeding • Breeders need genetic markers for effective & rapid breeding • Faba bean is an “orphan crop” with poorly developed genetics • Our goals
• develop effective markers & genetic maps • transfer marker systems and methods to FCRI partners • build capacity to use marker technologies for screening
genetic resources & in breeding • build capacity to increase crop production for new cultivation
areas in Egypt • thereby expand collaborative network (virtuous cycle).
Marker polymorphism
Genetic map
Genetic mapping
Mapped gene
needed for key sustainability traits
Mapping population X
markers
• Genotyped in the lab (Dr. Naglaa) • Phenotyped in the field (Dr. Magdy
Capacity building and knowledge transfer
Seminars to train in principles
PT
PT
LTR LTR
LTR LTR
IRAP marker method
• Exploits highly polymorphic component of genome • Easy to assay using commonly available lab equipment • Low-throughput (100s of samples, markers)
Primer 20130910 Faba bean: IRAP marker polymorphism
Training at Viikki
Capacity building and knowledge transfer
Lab training
Capacity building and knowledge transfer
Equipment setup
Genotyping by Sequencing (GBS): concept
GBS flow scheme
Faba bean GBS: first data
-mC
-mC
-mC parent 2
parent 1
offspring
Comparison to assembled soybean genome
Trainee: Ola Abdelalim Trainer: Jaakko Tanskanen
Trainee: Asmaa M. A. Abdul-Aziz
Faba bean: materials
Hamid Khazaei & Fred Stoddard (UH): Mélodie/2 x ILB 938/2 (ICARDA) 211 F5 RILs by single-seed descent
Faba bean: phenotyping of population In Viikki greenhouse by Khazai & Stoddard Response to water stress in parental lines, Mélodie/2 and ILB 938/2
Capacity building and transfer DNAs of faba bean mapping population arrive at FCRI, ARC
Faba bean: phenotyping of population In Ismailia under field conditions by Dr. Magdy
Evaluation under two saline conditions/locations: 2000 ppm 5500 ppm
Thanks for listening!
The Giza team