candidiasis: most common oppotunistic infection
DESCRIPTION
candidal growth in oral micro flora & its characteristic features.TRANSCRIPT
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/I INVESTIGATION FOR INVESTIGATION FOR CANDIDIASISCANDIDIASIS
Dr Usha Dr Usha
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CONTENTS :-
1. Definition
2.Classification
3. Clinical features and Differential
Diagnosis
4. Investigation for candiasis
5. Conclusion
6. references
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CANDIDIASIS
It is multiplicity of
disease caused by yeast
like fungi C. Albicans
-Most common oral
manifestation
-Other species……
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Classification:- 1.Acute Pseudomembranous Atrophic (Antibiotic sore mouth ) 2.Chronic Atrophic -Denture stomatitis (type I& II) -Median rhomboid glossitis -Angular chelitis cotinue……
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Hyperplastic Denture stomatitis (papillary type) Median rhomboid glossitis (nodular type) Candidal leukoplakia Multifocal 3.Mucocutaneous Syndrome associated Localized /Generalized 4.Immunocompromised state
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Predisposing Factors. The following predisposing factors for oral candidiasis have been defined by clinical observation:
1. Marked changes in oral microbial flora (due to the use of
antibiotics [especially broad-spectrum antibiotics], excessive
use of antibacterial mouth rinses, or xerostomia).2. Chronic local irritants (dentures and orthodontic appliances)3. Administration of corticosteroids (aerosolized
inhalant and topical agents are more likely to cause
candidiasis than systemic administration)
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4. Poor oral hygiene5. Pregnancy6. Immunologic deficiency — congenital or childhood (chronic familial
mucocutaneous candidiasis ± endocrine candidiasis syndrome [hypoparathyroidism, hypoadrenocorticism], and immunologic immaturity of infancy) — acquired or adult (diabetes, leukemia,
lymphomas, and AIDS) — iatrogenic (from cancer chemotherapy, bone
marrow transplantation, and head and neck radiation)7. Malabsorption and malnutrition
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CLINICAL FEATURES:-1.Acute pseudomembranous
candidiasis-Patchy white plaques or flecks on
mucosal surface.
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- can be scraped – leave area of erythema.
Painful lesion . Rapid onset of bad taste and loss of
taste Burning sensation of mouth and throat. Differential diagnosis- food debris habitual cheek biting
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Acute atrophic candidiasis presents as a red patch of atrophic or erythematous raw and painful
mucosa, with minimal evidence of the white pseudomembranous .
Antibiotic sore mouth- a common form of atrophic candidiasis
symptoms of oral burning, bad taste, or sore throat during
chronic iron deficiency anemia
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ACUTE ATROPHIC CANDIDIASIS
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CHRONIC ATROPHIC CANDIDIASIS DENTURE STOMATITIS (DENTURE SORE MOUTH)
.
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Three clinical stages :-
First stage consists of numerous palatal petechiae The second stage displays a more diffuse erythema involving most (if not all) of the denture-covered mucosa
The third stageincludes the development of tissue granulation or nodularity(papillary hyperplasia) commonly involving the central areas of the hard palate and alveolar ridges
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ANGULAR CHELITIS:-
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MEDIAN RHOMBOID GLOSSITIS
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Erythematous patches of atrophic papillae located in the
central area of the dorsum
more nodular,the condition is referred to as hyperplastic median rhomboid
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CHRONIC HYPERPLASTIC CANDIDIASIS:-
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CANDIDAL LEUKOPLAKIA
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CHRONIC MULTIFOCAL CANDIDIASIS:-
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CHRONIC MUCOCUTANEOUS CANDIDIASIS:-
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DIFFERENTIAL DIAGNOSIS
Leukoplakia Lichan planus chemical burns Gangrenous stomatitis Drug reaction Syphilitic mucous patches
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INVESTIGATION
Final diagnosis Design treatment Plan
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1.CULTURE:-Sample collection – from oral scraping Imprint culture :- Sample--- Piece of foam soaked in liquid growth
medium--- pressed on mucosal surface - then inoculated.
Culture medium:- Sabouraud’sAgar Blood Agar Cornmeal Agar CHROMagar Pagano-Levin Agar
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Inoculation At 37. For 48-72 hr Creamy white colonies, flat or hemispherical in shape ( Beer like aroma in sabouraud’s agar)
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2.MICROSCOPIC EXAMINATION BY DIFFERENT STAINING:-
----Heat fixed smear is prepared Staining by :- 20% kOH Gram staining Periodic acid-Schiff stain Brown and brenn stain Gomoris methanamine silver
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3. HISTOPATHOLOGIC EXAMINATION:- Biopsy specimen
fixing with 10% formalin + staining examination
hyphal penetration down to spinous cell layer, may invade entire epithelium,
connective tissue.
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4.HUMAN SALIVARY MUCIN INVESTIGATION:-
MUC7-12-mer-L and 12-mer-D peptide ---- show potent antifungal activity ---- Their level is detected.
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5.Rapid identification by DNA probe:-
- species specific probes are used to differentiate
C. dubliniesis .
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6.RAPID IDENTIFICATION BY ORAL RINSE SOLUTION:-
Rinsing of mouth with 10 ml of sterile
phosphate buffered saline
Collect saliva after 1min.
Centrifuge for 10 min.
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Incubation in serum at 37.C for 3 hr.
Germ tubes are seen.
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7. IDENTIFICATION BY SPECIFIC PROBES:-
-By polynucleotide and Oligonucleotide
probes.
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8.ELECTROPHORESIS & WESTERN BLOT TECHNIQUE:-
Sodium dodecyl SO4 -2 polyacrylamide gel electrophoresis & western blot
demonstrate outer cell wall layer of candida