Submitted by: Jonathan Duckworth Contact Number: 01223 394235

Candidate Name:

Candidate 21524

Location: Cambridgeshire

Salary Expectations: £28,000+

Current Role:

Molecular and Cellular Scientist

Desired Role:

Research Scientist

Notice Period:

1 month (current contract finishes on June 29th

)

Relevant Experience/Skills For Role:

A highly experienced molecular biologist with recent cell culture expertise. In his current role he provides high quality cell culture and optimisation of mammalian cell lines (e.g. HEK, CHO etc). He has a strong Pharmaceutical background and has provided key molecular support to Discovery projects. Aside from this he has excellent experience of several key techniques:

- Western Blotting - FAC’s sorting - SDS-page

Due to finish his fixed term contract in June 2014 and is he keen to stay in the Cambridge area.

Candidate 21524

PERSONAL PROFILE

An adaptable Biochemist and Molecular Biologist with an extensive scientific knowledge and expertise gained in Academia and the Pharmaceutical Industry.

CURRENT POSITION

Bench Scientist, Neusentis (Pfizer), Gt Abington, Cambridge July 2011 to present SRG Contractor: To provide key support for many projects within the electrophysiology group.

Key Responsibilities

Mammalian Cell Culture: Provide consistently high quality cells for a number of different projects and electrophysiology platforms. Cell culture and optimisation of difficult mammalian cell lines. Conduct QC, culture optimisation and cell banking.

Experience of automated e-phys platforms (Q-patch)

Devising transfection strategies (Lipofectamine): creation of transient and stable (constitutive and inducible) cell lines

Provide Molecular Biology support for various projects: cloning, sequence analysis, creating cell lines, Site-directed mutagenesis.

Lab manager (and safety steward) for the cell culture lab & Fire Steward.

CAREER HISTORY

Molecular & Cellular Biologist, WRD, Pfizer Ltd, Sandwich, Kent 2008 – 2011 SRG Contractor: Provided key support with Molecular Biology and Cell Culture for projects within the Pain research group

Molecular Biologist, VMRD, Pfizer Ltd, Sandwich, Kent 2007 – 2008 SRG Contractor: Provided key Molecular Biology support for projects within Veterinary Medicine Research and Development.

Research Officer, University of Wales, Bangor, Gwynedd, Wales 2003 – 2006 Postdoctoral Position: Identify and characterise the members of a protein super-family (in Arabidopsis) using standard Molecular Biology, Biochemistry and Microbiological techniques.

Gartnavel General Hospital (Molecular Biology), Glasgow, Scotland (3 months) 1998 Screened human DNA samples for the genetic disease haemachromatosis.

RESEARCH EXPERIENCE

Key Research Experience

DNA & RNA purification from plant, animal and human tissues, PCR, TAIL PCR, RT-PCR, qRT-PCR (Taqman), RACE, Site-directed mutagenesis, in vitro transcription and Northern, Southern and Western blotting.

Mammalian and Plant Cell Culture of adherent and suspension cell lines; also ex-vivo culture of bovine blood.

Transient and stable transfections (electroporation and lipofectamine) of mammalian and plant cell culture. Also FACS sorting and clonal selection of mammalian cell lines

Carbon-14 feeding studies, metabolite purification and analysis.

Creation of vector constructs for Sequencing, Transfection and High throughput cloning (Gateway system),

Creation of gene-GFP constructs, their transfection and analysis with Confocal microscopy.

Screening mutant populations and molecular characterisation of mutations.

Heterologous expression of protein in E. coli and purification of it using His-tag.

Characterisation of protein function through SDS-PAGE and enzyme assays.

Organised Work Experience at the following places:

Bishop Auckland General Hospital, County Durham 1994 Experience of Pathology Labs (Heamatology, Biochemistry, Cytology, Microbiology).

Great Lakes Chemical (Ltd), Newton Aycliffe, County Durham 1994 Analysing batches of samples for actual orders following standard protocols in QC labs.

EDUCATION

PhD in Biochemistry (University of Wales, Bangor) 1999 – 2003

BSc (Honours) in Biochemistry (2:i), (University of Glasgow) 1995 – 1999 (The course involved 2 years of Undergraduate Chemistry)

3 A-Levels: Biology, Chemistry and Physics 1993 – 1995 (Ferryhill Comprehensive School)

PERSONAL DEVELOPMENT

Supervisory Experience:

Supervising Industrial Trainees in Cell Culture (Pfizer) 2011 – 2014

Supervising Industrial Trainees in Molecular Biology (Pfizer) 2007 – 2011

Supervising undergraduate project student 2003 – 2004

Lab Demonstrator to undergraduates and marking of their lab reports 2000 – 2003

IT Skills:

Proficient with computers and competent with MS office (Word, PowerPoint and Excel), Adobe Photoshop and Databases.

Internet research databases (eg NCBI Genbank) and Internet tools (eg for DNA sequence manipulation).

Personal:

Carer for wife with long term illness 2006 - 2013

BBSRC Biotechnology Young Entrepreneur Scheme (four-man team) 2004

Elected committee member for Glasgow Gaming Society 1998 – 1999 (Organising venues and refereeing games for society members)

Student Representative for Biochemistry (2nd and 3rd Years) 1997 – 1998

Month-long expedition to Spitsbergen (Arctic) 1996

Full Clean Driving Licence.

INTERESTS & HOBBIES

Genealogy; Landscape photography; Baking; fell walking; Latin American/Ballroom/Scottish country dancing; active member of local church; writing short stories; visiting museums and English Heritage properties.

PUBLICATIONS

On request Conference Oral Presentations Society of Experimental Biology, Main Meeting, (Canterbury, UK) 2006 Plant Science Wales, (Aberystwyth, UK) 2001

Conference Poster Presentations Society of Experimental Biology, Main Meeting (Canterbury, UK) 2001 & 2006 Plant Science Wales (Aberystwyth, UK) 2003 XIIIth International Arabidopsis Conference (Seville, Spain) 2002

References

References available on request