Submitted by: Jonathan Duckworth Contact Number: 01223 394235

Candidate Name:

Candidate 116168

Location: Cambridge

Salary Expectations: Currently £31,500

Current Role:

Research Scientist

Desired Role:

Scientist/Senior Scientist

Notice Period:

1 Month

Relevant Experience/Skills For Role:

Holds a PhD in Biochemistry and Molecular Biology, work he has complemented with over 3 years research experience within industrial laboratories. Able to demonstrate experience in the following areas:

Mammalian and Bacterial cell culture experience.

Confident developing and validating enzymatic, spectroscopic, molecular and cellular assays within a drug discovery environment. (ELISA, qPCR, rtPCR, western blotting).

Protein expression, purification and characterisation.

Troubleshooting technical issues and a creative approach to problem solving.

Excellent communication skills.

Experience working under GMP Happy in his current role but is considering positions elsewhere which will offer further development.

Candidate 116168

Professional Experience: March 2013 – Present Isogenica, Little Chesterford Conducting research in a busy biopharmaceuticals laboratory as part of team using various Molecular biology techniques and display selection techniques in the discovery and development of cutting edge antibody technology to be used to target drugs for the treatment of several disease areas. My duties include, but are not restricted to, the following:

Phage and CIS-display to screen antibody libraries

Regular presenting of information and data

Library validation

Immunoassay development (ELISA and Western blotting)

Eukaryotic and bacterial cell culture

Protein stability studies, e.g. Sypro Orange.

RT-qPCR January 2011 – March 2013 MedImmune (SRG), Cambridge Similar to my current role at Isogenica but also included using ribosome display and FMAT and the building of optimisation libraries using Kunkel and error-prone (EP) PCR techniques. Education: 2005 – 2010 School of Chemistry, University of East Anglia PhD in Biochemistry and Molecular Biology Title: In vitro and in vivo studies of thio:disulphide oxidoreductase ResA from Bacillus subtilis and Streptomyces coelicolor. Supervisor: Dr Nick Le Brun Project:

In vivo investigation of key amino acid residues in B. subtilis ResA.

Design, over expression, purification and in vitro studies of the thiol:disulfide oxidoreductase ResA from S. coelicolor in Escherichia coli.

Generation and preliminary studies of a resA gene knockout in S. coelicolor. All three project elements were designed to further understand the importance and role of the active site or near active site residues, and to compare the role and properties of ResA homologues. Funded by the EPSRC. 2002 – 2005 School of Biological Sciences, University of East Anglia BSc. Molecular Biology and Genetics, Upper Second Class Dissertation Project: Investigation of proteins that interact with the leader sequence of periplasmic nitrate reductase from Escherichia coli. Modules studied include; Advanced Molecular Biology, Plant Molecular Biology, Molecular Microbiology, Parasitology and Microbial Physiology and Biotechnology. Research techniques:

Selection technologies e.g. phage, CIS and ribosome display (with mammalian cells, purified antigen, epitope competition elution, internalised elution), library design and creation

Diagnostic immunology techniques e.g., ELISA and Western blotting

Protein purification, including design of expression vectors, over-expression in cell

culture and purification from cell lysate using the AKTA purification platform and column chromatography (SEC, IEX, IAC, Unicorn).

Cell binding assays and screening e.g. FMAT

Spectroscopic assays including Sypro Orange, UV-visible absorbance and fluorescence methods to calculate protein unfolding temperatures, pKa values and redox potentials

Genetic manipulation, including recombineering, PCR amplification and site directed mutagenesis, as well as B. subtilis and S. coelicolor genetics.

SDS-PAGE and haem staining

Bacterial transformation using chemical competence, electroporation and conjugative gene transfer.

Analysing DNA and protein sequence data

Enzymatic assays, e.g. cytochrome c oxidase assay.

Cell culture

Data imaging, manipulation and analytical software (Blaze, PYMOL, Origin, Prism, Vector NTI, Spotfire)

Research Experience: Lund University, Sweden.

As part of my PhD studies, in December 2008, I was granted the opportunity to visit and conduct research in the Department of Cell and Organism Biology at Lund University, Sweden, under the guidance of Prof. Lars Hederstedt

Other Experience Post Graduate Demonstrating

During the course of my PhD I was involved with the supervision of undergraduate practical experiments. This involved preparing experimental procedures and materials, as well as tuition in the theory and techniques involved.

2002 – 2005 Norwich Union Life Outbound Calling Customer Services Representative

I was responsible for contacting existing customers of a life insurance provider to ensure their needs and requests were met.

I was involved in the training and coaching of other employees. IT Skills I have extensive knowledge of the major Microsoft programs including Word, Excel and Powerpoint. I am also competent in the use of proteomic and genetic analytical and visualisation tools and advanced internet search engines as well as data analysis software including Origin, Prism, Vector NTI and Spotfire. I have a full, clean, driving licence. Publications: On request