candida.pdf
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BY2012 Microbiology Gallery of Candida
CandidaCandida ArtArt
CandidaCandida albicansalbicans
Diagram as a budding yeast
Candida Candida albicansalbicans and Oral Thrushand Oral Thrush
Source: Atlas of Clinical Oral Pathology, 1999
Candida Candida albicansalbicans and Oral Thrushand Oral Thrush
Fungal DimorphismFungal Dimorphism Candida Candida albicansalbicans
Yeast phase – normal flora [Gram stain]
Hyphal phase - infection
Yeasts growing at 30°C on agar. Filamentous hyphae produced when growing at 37°C in vivo causing invasion of epithelial tissue.
CandidaCandida –– BuccalBuccal Epithelial CellsEpithelial Cells
Hyphae
Yeast cells
Epithelial cell
Candida Candida albicansalbicans InfectionInfection
Gram-stain of vaginal smear showing Candida albicans and epithelial cells with many Gram-negative rods in background
blastospores
hypha
nucleus epithelial cell
Candida Candida albicansalbicans
Scanning electron micrograph of C. albicans germ tube penetrating living tissue (arrowed)
Candida Candida albicansalbicans
Budding yeast
Pseudohypha
Phase contrast micrographs of C. albicans
Candida Candida albicansalbicans
Wet mount - phase contrast microscopy
Gram stain
Candida Candida albicansalbicans
Stained with fluorescein-labelled antibodies – UV microscopy
Candida Candida –– FeaturesFeaturesBudding yeast – Wet mount
Budding yeast – Gram stain
Germ tube
Chlamydospores
CandidaCandida PseudohyphaePseudohyphae ProductionProduction
CandidaCandida ––Adherence to Adherence to BuccalBuccal (Cheek) Epithelial Cells(Cheek) Epithelial Cells
CandidaCandida –– Cell Surface FeaturesCell Surface Features
Negative staining Freeze etchingThe outer cell wall layer is composed mainly of mannoproteins
covalently bound to ß-1,6- or ß-1,3-glucan and appears as a dense network of radially projecting fibrils
Candida Dimorphism
Germ Tubes of Germ Tubes of Candida Candida albicansalbicans
Colourised scanning electron micrograph of C. albicans yeast cells producing germ tubes
Candida Candida albicansalbicans forming forming HyphaeHyphae on Corn Meal Agaron Corn Meal Agar
Yeast cells
Hyphae
ChlamydosporesChlamydospores of of Candida Candida albicansalbicans (low power)(low power)
ChlamydosporesChlamydospores of of Candida Candida albicansalbicans
Black arrows = Chlamydospores; White arrows= Suspensor cells
ChlamydosporesChlamydospores ofof Candida Candida albicansalbicans
A B C
White arrows = Chlamydospores
Black arrows = Suspensor cells
ChlamydosporesChlamydospores of of Candida Candida albicansalbicans
Stained with Gram’s stain
Hypha
Chlamydospores
ChlamydosporesChlamydospores of of Candida Candida dubliniensisdubliniensis
Note multiple chlamydospores on the tips of the suspensor cells (arrowed)
ChlamydosporesChlamydospores of of Candida Candida dubliniensisdubliniensis
P = Pseudohypha; S = Suspensor cell
Chlamydospores occur in groups (arrowed)
CandidaCandida Identification Identification BBL CHROM BBL CHROM Candida Candida AgarAgar
CandidaCandida Identification Identification BBL CHROM BBL CHROM Candida Candida AgarAgar
BBL CHROM BBL CHROM Candida Candida AgarAgar
C. tropicalis C. albicans
C. dubliniensis C. glabrata C. parapsilopsis
C. krusei
BBL CHROM BBL CHROM Candida Candida AgarAgar
C. albicans C. tropicalisC. krusei C. glabrata
CandidaCandida Identification Identification HARDY CHROM HARDY CHROM Candida Candida AgarAgar
Candida glabrata Candida krusei
Candida albicans Candida tropicalis