INSTRUCTIONS

BX45AROUTINE MOTORIZED

MICROSCOPE

A X 9 9 3 6

This instruction manual is for the Olympus Routine Motorized Microscope Model BX45A.To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use ofthis microscope, we recommend that you study this manual thoroughly before operating the micro-scope.Retain this instruction manual in an easily accessible place near the work desk for future reference.

BX45A

CONTENTS

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5-6

7-15

IMPORTANT — Be sure to read this section for safe use of the equipment. —

1 NOMENCLATURE

2

3 USING THE CONTROLS

TRANSMITTED LIGHT BRIGHTFIELD OBSERVATION PROCEDURE

3-1 Microscope Base ................................................................................................................................................................................... 7

3-2 Focusing Block .......................................................................................................................................................................................... 8

1 Voltage Indication 2 Using the Light Intensity Preset Switch

3 Using the Filter

3-3 Motorized Revolving Nosepiece ............................................................................................................................ 9-10

3-4 Stage ............................................................................................................................................................................................................ 10-11

3-5 Observation Tube ...................................................................................................................................................................... 12-13 1 Adjusting the Interpupillar Distance 2 Adjusting the Diopter

3 Using the Eye Shades 4 Using Eyepiece Micrometer Disks

5 Adjusting the Tilt

1 Adjusting the Confocality of Objective 2 Centering the Objective

3 Switching the Objective

1 Placing the Specimen 2 Adjusting the X/Y-Axis Knob Tension

3 Rotating the Stage

1 Replacing the Fine Adjustment Knob 2 Adjusting the Coarse Adjustment Knob Tension

3 Pre-focusing Lever

1-3

Correct assembly and adjustments are critical for the microscope to exhibit its full performance. If you are going toassemble the microscope yourself, please read chapter 8, “ASSEMBLY” (pages 23 to 25) carefully.

ASSEMBLY — See this section for the replacement of the light bulb. —

3-6 Condenser .............................................................................................................................................................................................14-15 1 Centering the Condenser

3-7 Marker (U-MARKER) .......................................................................................................................................................................... 15

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5 TROUBLESHOOTING GUIDE

6 SPECIFICATIONS

7 OPTICAL CHARACTERISTICS

8

PROPER SELECTION OF THE POWER SUPPLY CORD ................................................................. 26-27

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20-21

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TRANSMITTED LIGHT SIMPLIFIED POLARIZED LIGHT OBSERVATION

1

IMPORTANT

This microscope employs a UIS (Universal Infinity System) optical design, and should be used only withUIS eyepieces, objectives and condensers for the BX2 series. (Some of the modules designed for the BXseries are also usable. For details, please consult Olympus or the catalogues.)

SAFETY PRECAUTIONS

1. Install the microscope on a sturdy, level table or bench so as not toblock the air vents on the underside of the base.Do not place the microscope on a soft surface where the microscopemay go down, for this blocks the air vents and may cause fire hazard.

2. This microscope uses air cooling based on natural convection. It shouldtherefore be installed so that spaces of 10 cm or more around themicroscope’s rear and side panels as well as the heat sink holes on thelamp socket.

3. To avoid potential shock hazards and burns when replacing the lightbulb, set the main switch @ to “ ” (OFF) then disconnect the powercord from the wall outlet in advance. Whenever you replace the bulbduring use or right after use, allow the lamp socket ² and bulb to coolbefore touching.

Designatedhalogen bulbs

6V30WHAL (PHILIPS 5761)

#The microscope also incorporate a fuse (this should be replacedby the manufacturer or authorized agent).

4. Make sure to set the voltage selector switch (³ in Fig. 2) to your localvoltage. (The switch has been set to 220-240 V before factory shipment.)

5. For the power cords of the AC adapter of the motorized revolving nose-piece and of the microscope frames, always use the power cord pro-vided by Olympus. If no power cord is provided, please select the properpower cords by referring to the chapter “PROPER SELECTION OF THEPOWER SUPPLY CORD” at the end of this instruction manual. If theproper power cords are not used, product safety and performance can-not be guaranteed.

6. Always ensure that the grounding terminal of the microscope and thatof the wall outlet are properly connected. If the equipment is not grounded,Olympus can no longer warrant the electrical safety and performance ofthe equipment.

7. To prevent electrical shock or malfunction, never insert a metallic objectin the air vent holes.

8. If the power cord, AC adapter cord or hand switch cord is distributednear the lamp socket, the cord may be melted, leading to an electricalshock hazard. To prevent this, distribute the cords enough apart from thelamp socket.

9. After use, disconnect the plug of the AC adapter power cord from a wallpower outlet to ensure safety.

Fig. 1

Fig. 2

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BX45A

Safety Symbols

The following symbols are found on the microscope. Study the meaning of the symbols and always use the equip-ment in the safest possible manner.

Symbol Explanation

Indicates that the surface becomes hot, and should not be touched with bare hands.

Before use, carefully read the instruction manual. Improper use could result in personal injury tothe user and/or damage to the equipment.

Indicates that the main switch is ON.

Indicates that the main switch is OFF.

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Indicates that a DC supply (from the provided AC adapter) should be used.

Indicates the Hand Switch.

Warnings

Warning engraving and stickers are placed at parts where special precaution is required when handling and using themicroscope. Always heed the warnings.

Indicate the input jack.

Warning engravingposition:

Lamp socket(Warning against high temperature)

Warning stickerposition:

Microscope rear panel(Warning against high temperature)

If the warning sticker is contaminated or peeled off, consult your local Olympus representative for replacement.

3

1 Getting Ready

1. A microscope is a precision instrument. Handle it with care and avoidsubjecting it to sudden or severe impact.

2. Do not use the microscope where it is subjected to direct sunlight, hightemperature and humidity, dust or vibrations. (For the operating condi-tions, refer to chapter 6, “SPECIFICATIONS”.)

3. Do not attempt to switch the motorized revolving nosepiece manually.(This will cause malfunction.)

4. The BX45A microscope has been designed for routine inspections andis not suitable for photomicrography.

5. When moving the microscope, carefully carry it by holding it by the handleon the rear panel and the handle and the base (Weight: approx. 14 kg).

#Do not hold the microscope by the stage, coarse/fine adjustmentknob or observation tune. Otherwise, the microscope may be dam-aged.

6. The BX45A can be used with only one single intermediate attachment.

2 Maintenance and Storage

1. Clean all glass components by wiping gently with gauze. To remove fingerprints or oil smudges, wipe with gauze slightlymoistened with a mixture of ether (70%) and alcohol (30%).Since solvents such as ether and alcohol are highly flammable, they must be handled carefully. Be sure to keepthese chemicals away from open flames or potential sources of electrical sparks –– for example, electricalequipment that is being switched on or off. Also remember to always use these chemicals only in a well-venti-lated room.

2. Do not attempt to use organic solvents to clean the microscope components other than the glass components. To cleanthem, use a lint-free, soft cloth slightly moistened with a diluted neutral detergent.

3. Never attempt to disassemble any part of the microscope.4. When not using the microscope, set the main switch to “ ” (OFF), make sure that the lamp socket is cool enough and

keep the microscope covered with a dust cover.

3 Caution

If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition,the equipment may also be damaged. Always use the equipment as outlined in this instruction manual.

The following symbols are used to set off text in this instruction manual.: Indicates that failure to follow the instructions in the warning could result in bodily harm to the

user and/or damage to equipment (including objects in the vicinity of the equipment).# : Indicates that failure to follow the instructions could result in damage to equipment.} : Indicates commentary (for ease of operation and maintenance).

Fig. 3

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BX45A

1 NOMENCLATURE

}If you have not yet assembled the microscope, read chapter 8, “ASSEMBLY” (pages 23 to 25).

Interpupillar distance scale(Page 12)

Diopter adjustmentring (Page 12)

Slider insertion slot

Motorized revolvingnosepiece

Specimen holder(Page 10)

Aperture iris diaphragmknob (Page 15)

Brightness adjustment knob (Page 7)(Lamp voltage adjustment system)

Field iris diaphragm dial (Page 14)

Filter slider (Page 7)

Coarse adjustment tensionadjustment ring (Page 8)

Allen screwdriver(accommodation position)

Centering knob(accommodation position)

Main switch

Pilot LED

Light intensity presetswitch (Page 7)

Light intensity presetadjustment screw

(Page 7)

Y-axis knob (Page 11)

X-axis knob (Page 11)

Objective switching button (Page 10)

AC adapterC-6AC

Hand switch

5

TRANSMITTED LIGHT BRIGHTFIELD OBSERVATIONPROCEDURE

}Make sure that the AC adapter @, hand switch ² and power cords are connected properly.}Perform the objective confocality adjustment and centering (page 9) before proceeding to the following.

(Controls Used) (Page)

Set the main switch to “ I ” (ON).

Engage a low-power objective in the light path.

Place the specimen on the stage.

Bring the specimen in focus.

Adjust the brightness.

Adjust the interpupillary distance.Adjust the diopter.Adjust the optical axis.

Adjust the aperture iris diaphragm and field irisdiaphragm.

Engage a high-power objective inthe light path.

Insert the required filter.

Adjust the brightness.

Start observation.

³Main switch

|Objective switching button (P. 10)

ƒSpecimen holder (P. 10)…X/Y-axis knobs (P. 11)

†Coarse/fine adjustment knobs (P. 8)

‡Brightness adjustment knob (P. 7)

ŠBinocular tube‰Diopter adjustment ring (P. 12)‹Condenser height adjustment lever (P. 14)ŒCondenser centering screw (P. 14)

™Aperture iris diaphragm knob (P. 15)šField iris diaphragm dial (P. 14)

|Objective switching button (P. 10)

›Filter (P. 7)

‡Brightness adjustment knob (P. 7)

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BX45A

} Make a photocopy of the observation procedure pages and post it near your microscope.

Centering knobs

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Also used forcentering knobs

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USING THE CONTROLS

3-1 Microscope Base

1 Voltage Indication (Fig. 4)

1. Turn the brightness adjustment knob @ clockwise to increase the volt-age and make illumination brighter.

2. The numerals ² around the knob indicate the approximate voltage.

2 Using the Light Intensity Preset Switch (Fig. 5)

}The light intensity preset switch @ makes it possible to limit the lightintensity to a preselected level regardless of the position of the bright-ness adjustment knob. The light intensity preset switch has been presetbefore factory shipment to about 4 V.

1. Press the light intensity preset switch @ to the ON position. (The switchlights when it is ON.)

2. Using a small flat-blade screwdriver, turn the preset adjustment screw ²to obtain the required light intensity. Turning the screw clockwise increasesbrightness.

3. When the light intensity preset switch is set to OFF, the brightness returnsto the level set by the brightness adjustment knob.

}While the light intensity preset switch is ON, turning the brightness ad-justment knob does not affect brightness.

3 Using the Filter (Fig. 6)

}Insert only one 32 mm filter @ in the filter slider ² then engage the filterslider in the light path.

Usable Filters and Applications

32LND6 Light intensity adjustment filter which ad-justs the brightness of the light source.(Transmittance: 6%)

32LND25 Light intensity adjustment filter which ad-justs the brightness of the light source.(Transmittance: 25%)

32C (provided)/32LBD Daylight filter/color temperature conversionfilter.

32IF550 Green filter which enhances contrast inmonochrome photographs.

Fig. 4

Fig. 5

Fig. 6

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BX45A

3-2 Focusing Block

#The BX45A microscope features a low stage height. The user is recommended to take care so that the user’s handor finger does not interfere with the stage when turning the coarse adjustment knob.

1 Replacing the Fine Adjustment Knob (Fig. 7)

}The fine adjustment knob has been attached on the right side at thefactory.

}The fine adjustment knob is designed detachable to prevent interferencewith hand during manipulation of the X/Y-axis knobs. Usually attach theknob on the opposite side to the X/Y-axis knobs.

1. Remove the rubber cap @ by puling it out.2. Loosen the clamping screw ² and remove the fine adjustment knob ³.3. Remove the seal from the fine adjustment knob screw hole on the other

side and attach the new knob by reversing the removal procedure.4. Attach one of the provided seals on the screw hole ƒ of the fine adjust-

ment dial ³, from which the fine adjustment knob has been removed.}The fine adjustment dial | can be operated with your fingertip or finger

surface.

2 Adjusting the Coarse Adjustment Knob Tension (Fig. 8)

#The rotation tension of the coarse adjustment knob should be ad-justed using the tension adjustment ring.The coarse adjustment knob tension is preadjusted for easy use. How-ever, if desired, you can change the tension using the tension adjust-ment ring @. Turning the ring clockwise in the direction of the arrowincreases tension, and vice versa.The tension is too low if the objective drops by itself or focus is quicklylost after adjustment with the fine adjustment knob. In this case, turn thering in the direction of the arrow to increase tension.

3 Pre-focusing Lever (Fig. 9)

}The pre-focusing lever ensures that the objective does not come in con-tact with the specimen and simplifies focusing.After focusing on the specimen with the coarse adjustment knob, turnthis lever @ in the direction of the arrow and lock; the lower limit of coarseadjustment movement is set at the locked position.After changing a specimen, refocusing is easily accomplished by rotat-ing the coarse adjustment knob to reach the pre-focused position, thenmaking fine adjustments with the fine adjustment knob.

}The objective’s vertical movement activated by the fine adjustment knobis not locked.

#If you want to lower the objective to the lower limit, release the lockin advance.

Fig. 7

Fig. 8

Fig. 9

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3-3 Motorized Revolving Nosepiece

1 Adjusting the Confocality of Objective (Fig. 10 & 11)

}To maintain the accurate focusing even after the objective is switched,the height of the objective with the higher focal point is corrected byattaching a washer.Fifteen washers with three kinds of thickness (10, 30 and 50 µm), threeper kind, are provided with the microscope.

1. Engage the objective on the right in the light path and adjust focusing.For use in the confocality adjustment, read the scale indication of the fineadjustment knob (1 scale graduation: 1 µm).

2. Engage the objective on the left in the light path by operating the objec-tive switching button on the hand switch.Adjust focusing, read the scale indication of the fine adjustment knoband obtain the difference from the value read in step 1 above.

3. The objective to use the washer is determined by the rotation direction ofthe fine adjustment knob @. (Fig. 10) · Direction of the arrow: Objective on the left. · Opposite direction of the arrow: Objective on the right.

4. Remove the objective ², fit the washer | with the required thickness onthe screw ³, then attach the objective again.

5. Switch the objective and confirm that confocality is implemented.#Depending on the objective, perfect confocality may sometimes not

be achieved.

2 Centering the Objective (Fig. 12)

}Use the 2 centering knobs @, which are also used in centering the con-denser.The centering mechanism is provided only for the objective on the right.

1. Adjust focusing using the objective on the left, then move the targetregion in the specimen on the center of the field.

2. Using the objective switching button on the hand switch, switch the ob-jective to that on the right.

3. Insert the 2 centering knobs @ into the threaded holes and turn them tomove the target in the objective on the center.

}After completing centering, store the centering knobs in the accommo-dation position on the right side panel of the microscope (page 4) so asnot to lose them.

Fig. 10

Fig. 11

Fig. 12

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BX45A

3 Switching the Objective (Fig. 13)

Each press of the objective switching button @ on the hand switch en-gages the left and right objective alternately.

How to Reset Error Which May Occur During Switching

}If the objective hits the specimen or cannot be switched in a normalperiod of time due to a foreign object, the protection circuitry is activatedto abort the switching operation.To release from this condition, set the main switch of the microscope to“ ” (OFF) then “ I ” (ON) again.Then press the objective switching button on the hand switch; the objec-tive on the right will be engaged in the light path.

3-4 Stage

1 Placing the Specimen

#The dimensions of the slide glass should be 26 x 76 mm with thick-ness of 0.9 to 1.4 mm, and the cover glass should have thickness of0.17 mm.

#When observing very large specimens, remove the specimen holderand place the specimen directly on the stage.

Microscopy with Double-Specimen Holder (Fig. 14)

1. Turn the coarse adjustment knob @ to raise the objective.2. Open the spring-loaded curved finger ² on the specimen holder and

place the specimen on the stage from the front.3. After placing the specimen as far as it will go, gently release the curved

finger.

Microscopy with Single-Specimen Holder (Fig. 15)

The specimen can easily be placed by sliding it into the specimen holderfrom the front.

Fig. 13

Fig. 14

Fig. 15

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2 Adjusting the X/Y-Axis Knob Tension (Fig. 16)

1. Hold the X-axis knob @ and slide the Y-axis knob ² up to expose theadjustment knobs.

2. Turning the X-axis adjustment knob ³ or Y-axis adjustment knob | clock-wise (in the direction of the arrow) increases the tension and counter-clockwise decreases it.

#If the tension is adjusted too tight, a creaking sound may be heardduring stage travel, and the stage stopping accuracy may be imper-iled.

CAUTIONAfter long hours of use, the stage guide may be devi-ated and the stage travel range may be decreased.However, this is not malfunction and can be correctedeasily as described below.

[Treatment] X-axis direction: Hold the specimen holder and move the stageguide to the left and right so that it hits thestoppers.

Y-axis direction: Hold the upper stage and move it to the frontand rear so that it hits the stoppers.

Rubber Stage Knob Caps (Optional)

}When the X/Y-axis knobs are fitted with the rubber knob caps, the knobscan be adjusted without slipping and fine adjustment is possible byholding the knobs with a very light force. The rubber knob caps alsoreduces fatigue after long hours of operation.The U-SHGT thick type (thickness 5 mm) and U-SHG thin type (thickness2 mm) rubber knob caps are available.To attach the knob rubber caps:First fit the larger rubber knob cap to the Y-axis (upper) knob from belowit, then fit the smaller rubber knob cap to the X-axis (lower) knob frombelow it.

3 Rotating the Stage (Fig. 17)

1. Slightly loosen the stage clamping screw @ using the Allen screwdriver.2. The stage can be rotated both clockwise and counterclockwise.#A click may be heard and felt during rotation. However, this is due to

the construction of the substage and does not indicate a malfunc-tion.

}The angle of rotation varies depending on the knobs.

Angle of Rotation

Clockwise Counterclockwise

Right-hand knobs 230° 20°

Left-hand knobs 20° 230°

Fig. 16

Fig. 17

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BX45A

3-5 Observation Tube

1 Adjusting the Interpupillar Distance (Fig. 18)

While looking through the eyepieces, adjust for binocular vision until theleft and right fields of view coincide completely. The index dot · indicatesthe interpupillary distance.

}Note your interpupillary distance so that it can be quickly duplicated.

2 Adjusting the Diopter (Fig. 19)

1. Looking through the eyepiece without the diopter adjustment ring, rotatethe coarse and fine adjustment knobs to bring the specimen into focus.

2. Looking through the eyepiece with the diopter adjustment ring @, turn itto focus on the specimen.

3 Using the Eye Shades (Fig. 20)

When Wearing Eyeglasses

Use with the eye shades in the normal, folded-down position. This willprevent the eyeglasses from being scratched.

When Not Wearing Eyeglasses

Extend the folded eye shades in the direction of the arrow to preventextraneous light from entering between the eyepieces and eyes.

4 Using Eyepiece Micrometer Disks (Fig. 21)

Eyepiece micrometer disk can be inserted into the WH10X-H (or WH10X)eyepieces.Use a 24 mm dia. x 1.5 mm micrometer disk.Following Fig. 21, remove the micrometer mounting frame ² from theeyepiece and place a micrometer disk @ into the mounting frame whilefacing down the disk surface with the indication.Re-attach the micrometer mounting frame in the original position.

Fig. 18

Fig. 19

Fig. 20

Fig. 21

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5 Adjusting the Tilt (with the U-TBI3) (Fig. 22)

}Adjust the height and tilt angle of the observation tube to obtain the mostcomfortable viewing position.Holding the binocular section with both hands, raise or lower it to thedesired position.

#Never attempt to force the binocular section past the upper or lowerstop position. Applying excessive force could destroy the limitingmechanism.

#When the U-TBI3 is used, part of the peripheries of the field of viewmay become dark as the aperture iris diaphragm is stopped downto approximately the minimum aperture.

#When the U-TBI3 is used in combination with an intermediate tube(U-EPA2), insufficiency in light may occur in the peripheral area ofthe field of image.

U-ETBI (Fig. 23)

The U-ETBI is an ergonomic observation tube with normal field, capableof tilting adjustment with an erect image. The eyepiece position can beadjusted by 45 mm toward the front and rear.

Fig. 22

Fig. 23

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BX45A

3-6 Condenser

1 Centering the Condenser (Figs. 24 - 26)

1. Turn the condenser height adjustment lever @ in the direction of thearrow to raise the condenser to its upper limit.

2. Focus on the specimen using a lower-power objective.3. Rotate the field iris diaphragm dial ² in the direction of the arrow so that

the diaphragm image comes inside the field of view.4. Turn the condenser height adjustment lever @ in the opposite direction

of the arrow to focus on the diaphragm image.5. Insert the condenser centering knobs ³ into the two condenser center-

ing screws and turn the knobs to move the iris diaphragm image to thecenter of the field of view.

6. Gradually open the field iris diaphragm. The condenser is properly cen-tered if the iris image is centered and inscribed in the field of view.

7. During actual use, open the field diaphragm slightly until its image cir-cumscribes the field of view.

}When centering is complete, screw the centering knobs into the storagepositions on the right side of the microscope frame. (P. 4)

Effects of Field Iris Diaphragm (Fig. 26)

The field iris diaphragm restricts the diameter of the beam of light enter-ing the objective and thus excludes extraneous light, improving imagecontrast. The diameter of the field iris should be adjusted for objectivepower to the extent that it just circumscribes the field of view.

}When using a 4X objective, set the field iris diaphragm at maximum.

Fig. 24

Fig. 25

Fig. 26

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Aperture Iris Diaphragm (Fig. 27)

· The aperture iris diaphragm determines the numerical aperture of theillumination system. Matching the numerical aperture of the illuminationsystem with that of the objective provides better image resolution andcontrast, and also increases the depth of focus.

· Since the contrast of microscope specimens is ordinarily low, setting thecondenser aperture iris diaphragm to between 70% and 80% of the N.A.of the objective in use is usually recommended. If necessary, adjust theratio by removing the eyepiece and looking into the eyepiece sleevewhile adjusting the aperture iris diaphragm knob (| in Fig. 24) until theimage shown in Fig. 27 is seen.

3-7 Marker (U-MARKER)

}By mounting the marker @ on the 10X objective, a desired point can bemarked with a one-touch operation.The marker can be manipulated with either the left or right hand accord-ing to the positioning of the 10X objective. (For details, refer to the sepa-rate instruction manual for the U-MARKER.)

}The objective for use in cytodiagnosis should be the combination of thePlan10XCY and Plan series objective or the PlanFl10XCY and PlanFl se-ries objective.

#To prevent the pen tip of the marker from drying up, always attachthe exclusive cap ² on the pen tip whenever it is not used.

Fig. 27

Fig. 28

Objective pupil

70-80%

30-20%

Aperture irisdiaphragm image

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BX45ATRANSMITTED LIGHT SIMPLIFIED POLARIZEDLIGHT OBSERVATION

}The U-ANT analyzer (or a module incorporating analyzer) and the BX45-PO polarizer are required for polarized light obser-vation.

1 Attaching the BX45-PO Polarizer (Fig. 29)

1. Remove the filter slider @.2. In the slot, insert the BX45-PO polarizer ² all the way.}The cross-Nicol (dark) position is obtained when the polarizer lever comes

right beside the microscope frame.

2 Attaching the U-ANT Analyzer (Fig. 30)

1. Remove the rubber cap from the slider insertion slot above the revolvingnosepiece.

2. Hold the U-ANT analyzer ² so that the surface with indications face up,and drop the analyzer into the dummy slider @ provided with the BX45-PO by alighting their index markings. (The analyzer will be fixed by mag-netism.)

3. Insert the dummy slider @ containing the U-ANT into the slider insertionslot and push the slider all the way to engage the analyzer in the lightpath.

}The analyzer can be disengaged from the light path by pulling the sliderout by one step.

3 Observation Method

1. Engage the objective in the light path.2. Turn the polarizer lever slightly so that the field of view is darkest (cross-

Nicol position).3. Place the specimen on the stage and adjust the focusing.4. Adjust the field iris diaphragm so that its image circumscribes the field of

view.5. The contrast may sometimes be enhanced by closing the aperture iris

diaphragm slightly.

Fig. 29

Fig. 30

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TROUBLESHOOTING GUIDE

Under certain conditions, performance of the unit may be adversely affected by factors other than defects. If problems occur,please review the following list and take remedial action as needed. If you cannot solve the problem after checking theentire list, please contact your local Olympus representative for assistance.

Problem Cause Remedy Page

1. Optical System

a) Bulb does not light. Bulb is burned out. Replace bulb. 24

Power cord is unplugged. Plug power cord into power outlet. 25

b) Bulb lights but the field of view isdark.

Aperture and field iris diaphragm are notwide enough.

Open them to appropriate sizes.14/15

c) Field of view is obscured or notevenly illuminated.

Objective is not engaged correctly in lightpath.

Press objective switching button again.10

Condenser is not centered. Center it. 14

Field iris diaphragm is closed too much. Open it sufficiently. 14

Lamp bulb is not installed correctly. Push halogen bulb terminals all theway into stop position.

24

d) Dirt or dust is visible in the field ofview.

Dirt/dust on eyepiece. Clean thoroughly.

3Dirt/dust on condenser top lens.

Dirt/dust on specimen.

e) Visibility of observed image is poor. · Image is not sharp. · Contrast is poor. · Details are poorly visible. · Darkfield observation is poorly vis-

ible (with flare tendency)

Objective in use is not designed for UISseries.

Replace with a specified objective forUIS series.

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Aperture iris diaphragm is closed toomuch.

Open it sufficiently.15

Dirt/dust on extremity of objective. Clean it thoroughly.

3Dirt/dust on specimen.

Dirt/dust on condenser top lens.

Thickness of slide glass or cover glassis inappropriate.

Replace with slide or cover glass withoptimum thickness.

10

f ) One side of image is blurred. Objective is not correctly engaged in lightpath.

Press objective switching button again.10

Stage is not installed properly. Detach and re-attach stage. –

Specimen is not placed correctly onstage.

Place specimen correctly on top sur-face of stage and fix specimen slidewith specimen holder.

10

g) Image looks like flowing. Objective is not correctly engaged in lightpath.

Press objective switching button again.10

Condenser is not centered correctly. Center it correctly. 14

h) Field of view can be brightened onlyslightly by increasing voltage intensity.

Condenser is not centered correctly. Center it correctly. 14

Condenser is too low. Adjust its height correctly. 14

2. Electrical System

a) Bulb intermittently lights and goesout.

Bulb is nearly burnt out. Replace bulb. 24

A cord or connector is not properly con-nected.

Connect cords and plugs securely.25

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BX45A

Problem Cause Remedy Page

b) Lamp bulb burns out soon afterlighting.

The bulb in use is not the specified lamp. Replace with a standard bulb.24

c) Brightness cannot be varied withbrightness adjustment knob.

Light intensity preset switch is set to ON. Set it to OFF. 7

Lamp bulb is not installed. Attach lamp bulb. 24

Lamp bulb is burnt out. Replace lamp bulb. 24

Lamp socket is not connected. Connect lamp socket. 24

d) LEDs do not light.Pilot lamp does not light.

Voltage selector switch is set to 220-240V.

Set is to 100-120 V position.1

e) Motorized revolving nosepiece willnot work.

Connection of AC adapter and/or handswitch cable on connector or power out-let side is incorrect.

Connect cables correctly.25

Motorized revolving nosepiece isstopped in the middle due to an error.

Reset error by following the error re-setting instructions.

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3. Coarse/Fine Adjustment Knobs

a) Coarse adjustment knob is tooheavy to rotate.

Rotation tension adjustment ring is tootight.

Loosen ring to an optimum tightness.8

Upward/downward coarse adjustment isattempted while pre-focusing lever islocked.

Release lock of pre-focusing lever.8

b) Objective drifts down by itself or fo-cus is lost due to slipping of fineadjustment knob during observa-tion.

Tension adjustment ring is too loose. Tighten ring to an optimum tightness.

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c) Coarse adjustment cannot bringobjective low enough.

Pre-focusing lever is locked on the up-per side.

Release lock of pre-focusing lever.8

d) Objective hits specimen before it isbrought into focus.

Specimen is placed upside down. Place specimen correctly.–

4. Observation Tube

a) Field of view of one eye does notmatch that of the other.

Interpupillary distance is incorrect. Adjust interpupillary distance. 12

Diopter adjustment is incorrect. Adjust diopter. 12

Different eyepieces are used on left andright.

Change on eyepiece to match theother so that both sides are the sametype.

–

You are not accustomed to parallel opti-cal axis.

When looking into eyepieces, do notstare at image from the beginning butsee the overall field of view. It is some-times recommended to turn your eyesaway from eyepieces, look far off andlook into eyepieces again.

–

5. Stage

a) Image shifts when you touch stage. Stage is not properly mounted. Clamp stage. 11

b) X-axis traverse stops midway. Specimen is not correctly positioned. Place specimen correctly. 10

c) X-axis and/or Y-axis knobs too tightor too loose.

Tension of X-axis and/or Y-axis knobs toohigh or too low.

Adjust tension.11

19

d) Stroke has reduced. Stage guide is deviated. Use treatment method described onpage 11.

11

Problem Cause Remedy Page

6. Marker

a) Marking is impossible. Pen has run out of ink. Replace pen. –

Stroke adjustment is incorrect. Re-adjust stroke. –

Pen tip is dry. Wipe pen tip with tissue paper andretry writing.

–

b) Slide glass slips out as a result ofmarking.

Stroke adjustment is incorrect. Re-adjust stroke.–

c) Pen service life is very short. Pen tip is dry. Cap pen whenever possible. –

20

BX45A

SPECIFICATIONS

Item Specification

1. Optical system UIS (Universal Infinity System) optical system

2. Illumination system Transmitted Koehler illuminator built in.6 V, 30 W halogen bulb (pre-centered) 6V30WHAL (PHILIPS 5761).(Average life time: Approximately 100 hr. when used as directed)Intensity control range: Below 2 V to 5.9 V (continuously variable)Light preset switch: Preset voltage can be set arbitrarily between below 2 and 5.9 V.Rated input voltage: 100-120 V/220-240 V , 0.8 A/0.4 A, 50/60 Hz.Power consumption: 45 W.

3. Focusing system Revolving nosepiece height movement by roller guide (rack & pinion)Stroke per rotation: 0.1 mm (fine), 17.8 mm (coarse)Full stroke range: 15 mmCoarse adjustment pre-focusing leverRotation tension adjustment on coarse focus adjustment knob.

4. Motorized revolving nose-piece

2-position lateral swinging system (Rotation angle: 62°)Centering available for objective hole on the right.Operation by objective switching button on the hand switch.Mechanism for shock reduction in objective switching.Protection circuit (The motor is released in 3 sec. after penetration of a foreign object.)

5. AC adapter Rated input voltage: 100-120 V/220-240 V , 1.0 A/0.5 A

6. Observation tube Model U-BI30-2 U-TBI3 U-ETBI

Widefield binocular Widefield tilting binocu-lar

Widefield erected-imagetilting binocular

Field number FN 22

Tube tiltingangle

30°5°- 35°

(Continuously variable)0°-25°

(Continuously variable)

In terpupi l la rydistance adjust-ment

50 to 76 mm

7. Stage Model U-SVRC U-AVLC

Coaxial bottom right knob Coaxial bottom left knob

Rectangular ceramic-coated wire driven stage

Size 156 mm (Depth) x 191 mm (Width)

Cross move-ment mecha-nism

X/Y-axis knob tension adjustable.Movement range: X-axis 76 mm, Y-axis 52 mm

Specimen hold-ers (for single-specimen ob-servation)

U-HLS4U-HLST4

U-HRS4U-HRST4

Specimen hold-ers (for double-specimen ob-servation)

U-HLD4U-HLDT4

U-HRD4U-HRDT4

21

Item Specification

8. Condenser Abbe condenser, fixed.NA: 0.1 to 0.9Aperture iris diaphragm: Aperture iris diaphragm scale provided.Applicable objective magnifications: 4X to 40X

9. Operating environment · Indoor use. · Altitude: Max. 2000 meters · Ambient temperature: 5° to 40°C (41° to 104° F) · Maximum relative humidity: 80% for temperatures up to 31°C (88°F), decreasing linearly

through 70% at 34°C (93°F), 60% at 37°C (99°F), to 50% relative humidity at 40°C (104°F). · Supply voltage fluctuations: ±10% · Pollution degree: 2 (in accordance with IEC664) · Installation (overvoltage) category: II (in accordance with IEC664)

22

BX45A

OPTICAL CHARACTERISTICS

NOTE

Refer to the latest catalogue or consult your local Olympusrepresentative for the updated information on the eyepiecesand objectives that can be combined with this microscope.

Opticalcharacter Magni-

fication N.A.W.D.(mm)

Coverglassthick-ness

Reso-lution(µm)

Eyepiece

WHC10X (FN20)Remark

Objective Total mag.Depth of focus

(µm) Field of view

AchC Achromat*(FN20)

* Be sure to use the AchC series in combination with the WHC10X.

4X 0.10 28.90 — 3.4 40X 175 5.010X 0.25 6.30 0.17 1.34 100X 28.0 2.040X 0.65 0.62 0.17 0.52 400X 3.0 0.5

Name

Numerical aperture

Magnification

Mechanical tube length Cover glass thickness

The table below shows the optical characteristics of differenteyepiece and objective combinations. Objective specificationsare marked on the objective (as shown in the diagram on theright).

Opticalcharacter

Magni-fication

N.A. W.D.(mm)

Coverglassthick-ness

Reso-lution(µm)

Eyepiece

Remark

Objective

WH10X (FN22) WH15X (FN14)

Totalmag.

Depthof focus

(µm)

Fieldof view

Totalmag.

Depthof focus

(µm)

Fieldof view

10X 0.25 6.1 — 1.34 100X 28.0 2.2 150X 20.9 1.420X 0.40 3.0 — 0.84 200X 6.09 1.1 300X 4.64 0.740X 0.65 0.45 0.17 0.52 400X 3.04 0.55 600X 2.35 0.354X 0.10 22.0 — 3.36 40X 175 5.5 60X 85.8 3.5

10X 0.25 10.5 — 1.34 100X 28.0 2.2 150X 20.9 1.410XCY 0.25 10.5 — 1.34 100X 28.0 2.2 150X 20.9 1.420X 0.40 1.2 0.17 0.84 200X 6.09 1.1 300X 4.64 0.740X 0.65 0.56 0.17 0.52 400X 3.04 0.55 600X 2.35 0.354X 0.13 17.0 — 2.58 40X 127 5.5 60X 92.9 3.5

10X 0.30 10.0 — 1.12 100X 22.4 2.2 150X 16.5 1.420X 0.50 1.6 0.17 0.67 200X 7.00 1.1 300X 5.22 0.740X 0.75 0.51 0.17 0.45 400X 2.52 0.55 600X 1.93 0.354X 0.16 13.0 — 2.1 40X 99.5 5.5 60X 71.7 3.5

10X 0.40 3.1 0.17 0.84 100X 15.9 2.2 150X 11.5 1.420X 0.70 0.65 0.17 0.48 200X 4.65 1.1 300X 3.39 0.740X 0.85 0.2 0.11-0.23 0.39 400X 2.14 0.55 600X 1.62 0.35

1.25X 0.04 5.1 — 8.38 12.5X 872 17.6 18.75X 639 11.22X 0.08 6.0 — 4.19 20X 398 11.0 30X 287 7.0

40X 0.75 0.63 0 0.45 400X 1.66 0.55 600X 1.27 0.35

ND filter

Indicates the objective is provided with a correction collar.

AchAchromat(FN22)PlanPlan Achromat(FN22)

UPlanFlUniversal PlanApochromat(FN26.5)

UPlanApoUniversal PlanApochromat(FN26.5)PlanApoPlan Apochromat(FN26.5)UMPlanFlNo CoverUniversal PlanSemi-Apochromat(FN26.5)PlanFlPlanSemi-Apochromat(FN26.5)

10XCY 0.30 9.90 — 1.12 100X 22.0 2.2 150X 16.4 1.4

ND filter

23

ASSEMBLY

8-1 Assembly Diagram

The diagram below shows the sequence of assembly of the various modules. The numbers indicate the order of assembly.The module numbers shown in the following diagram are merely the typical examples. For the modules with which themodule numbers are not given, please consult your Olympus representative or the catalogues.

#When assembling the microscope, make sure that all parts are free of dust and dirt, and avoid scratching any partsor touching glass surfaces.Assembly steps enclosed in will be detailed on the subsequent pages.

}All assembly operations are possible by using the Allen screwdriver ( ) provided with the microscope.

EyepieceWH10X

Tube clampingscrew

Observation tubeU-BI30-2U-TBI3U-TR30-2U-ETBI

UIS objectivePlan10XCYPlanFI10XCY UIS

objectiveseries

MarkerU-MARKER

Specimen holderU-HLD-4U-HRD-4U-HLS-4U-HRS-4

Specimen holderclamping knob

StageU-SVRCU-SVLCU-SRG2

Stage clampingscrew

Intermediate attachmentU-EPA2U-DA, etc.

Hand switch

AC adapter

Halogen bulb6V30WHAL

Power cord

Lamp socketU-LS30-4

Microscope frameBX45ATF

Power cord

24

BX45A

8-2 Detailed Assembly Procedures

1 Attaching the Lamp Socket (Fig. 31 & 32)

The applicable bulb is the 6V30WHAL (PHILIPS 5761) halogen bulb.

1. Hold the bulb @ by wrapping it with a piece of gauze, and push the bulbpins ² all the way into the pin holes ³ of the lamp socket.

#To prevent reduced bulb life or cracking, do not touch the bulb withbare hands. If fingerprints are accidentally left on the bulb, wipe thebulb with a soft cloth.

2. Align the guide pins | with the guide holes ƒ on the rear of the micro-scope frame, then push the plug … all the way into the socket † so thatthe guide pins fit in the guide holes.Caution for Bulb Replacement During After UseThe bulb, lamp socket and areas near these will be extremely hotduring and right after use.Set the main switch to “ ” (OFF), disconnect the power cord fromthe wall outlet, then allow the old bulb and lamp socket to coolbefore replacing the bulb with a new one of the designated type.

5 Attaching the Objective (Fig. 33)

Screw in a lower-power objective and higher-power firmly into the motor-ized revolving nosepiece.

}When switching a 10X and 40X objectives alternately during observation,it is recommended to use the PlanFI10XCY or Plan10XCY because theseobjectives incorporate built-in ND filters so it is not required to adjust thebrightness when the objective is switched to the 40X, thereby improvingthe ease of operation.

Inserting the ND filter for Plan10X objective

}The ND filter (12.6ND25) for the Plan10X objective provides the objectivewith the same characteristics as the CY specification.

1. Drop in the ND filter @ in the 10X objective. (Fig. 33)2. To fix the ND filter, drop in the ring spring ² provided with the filter by

narrowing the ring. (Fig. 33)

Fig. 31

Fig. 32

Fig. 33

@

²

³

|ƒ

…†

@

²

25

7 Attaching the Cables and Cords (Fig. 34 & 35)

Always use the AC adapter and its power cord provided by Olympus.If no power cord is provided with the microscope, please select theproper power cord by referring to chapter “PROPER SELECTION OFTHE POWER SUPPLY CORD” on next page.The cables and cords are vulnerable when bent or twisted. Neversubject them to excessive force.Make sure that the main switch of the BX45ATF is set to “ ” (OFF)before connecting cables and cords.

1. Insert the hand switch plug @ into the HS connector ² on the rear of themicroscope.

}The hand switch is provided with double-side adhesive tape. Use it whenyou want to install the hand switch in an easy-to-operate position.

2. Insert the AC adapter’s power output jack ³ into the microscope’s powerinput jack |.

3. Insert the power cord’s connector … into the AC adapter’s power inputconnector ƒ, then insert the plug Š of the AC adapter power cord into awall power outlet ‰.The power cord should be connected to a grounded, 3-conductorpower outlet. If the power outlet is not grounded properly, Olympuscan no longer warrant the electrical safety performance of the equip-ment.

4. Insert the power cord’s connector † into the microscope’s power inputconnector ‡.

5. Insert the plug Š of the microscope power cord into a wall power outlet‰.If a power cord, AC adapter cord or hand switch code comes incontact with the lamp socket or the surroundings, the cord maymelt down, causing electric shock hazards. Be sure to distribute thecords at enough distance from the lamp socket.

Fig. 34

Fig. 35

@ ²

³|

ƒ…

† ‡

Š ‰

26

BX45A

PROPER SELECTION OF THE POWER SUPPLY CORD

If no power supply cord is provided, please select the proper power supply cord for the equipment by referring to “ Specifications ” and“ Certified Cord ” below:CAUTION: In case you use a non-approved power supply cord for Olympus products, Olympus can no longer warrant the

electrical safety of the equipment.

Specifications

Voltage RatingCurrent RatingTemperature RatingLengthFittings Configuration

125V AC (for 100-120V AC area) or, 250V AC (for 220-240V AC area)6A minimum60°C minimum3.05 m maximumGrounding type attachment pulg cap. Opposite terminates in molded-on IEC con-figuration appliance coupling.

Table 1 Certified Cord

A power supply cord should be certified by one of the agencies listed in Table 1 , or comprised of cordage marked with anagency marking per Table 1 or marked per Table 2. The fittings are to be marked with at least one of agencies listed inTable 1. In case you are unable to buy locally in your country the power supply cord which is approved by one of theagencies mentioned in Table 1, please use replacements approved by any othere quivalent and authorized agencies inyour country.

Country Agency CertificationMark Country Agency

CertificationMark

Argentina

Australia

Austria

Belgium

Canada

Denmark

Finland

France

Germany

Ireland

IRAM

SAA

ÖVE

CEBEC

CSA

DEMKO

FEI

UTE

VDE

NSAI

Italy

Japan

Netherlands

Norway

Spain

Sweden

Switzerland

United Kingdom

U.S.A.

IMQ

MITI

KEMA

NEMKO

AEE

SEMKO

SEV

ASTABSI

UL

27

Table 2 HAR Flexible Cord

APPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODS

Approval Organization

Printed or embossed Harmoniza-tion Marking (May be located onjacket or insulation of internal wir-ing)

Alternative Marking UtilizingBlack-Red-Yellow Thread (Lengthof color section in mm)

Black Red Yellow

Comite Electrotechnique Belge(CEBEC)

Verband Deutscher Elektrotechniker(VDE) e.V. Prüfstelle

Union Technique de d´Electricite´(UTE)

Instituto Italiano del Marchio diQualita´ (IMQ)

British Approvals Service for ElectricCables (BASEC)

N.V. KEMA

SEMKO AB Svenska ElektriskaMaterielkontorollanstalter

Österreichischer Verband fürElektrotechnik (ÖVK)

Danmarks Elektriske Materielkontrol(DEMKO)

National Standards Authority of Ireland(NSAI)

Norges Elektriske Materiellkontroll(NEMKO)

Asociacion Electrotecnica YElectronica Espanola (AEE)

Hellenic Organization forStandardization (ELOT)

Instituto Portugues da Qualidade(IPQ)

Schweizerischer ElektroTechnischer Verein (SEV)

Elektriska Inspektoratet

CEVEC <HAR>

<VDE> <HAR>

USE <HAR>

IEMMEQU <HAR>

BASEC <HAR>

KEMA-KEUR <HAR>

SEMKO <HAR>

<ÖVE> <HAR>

<DEMKO> <HAR>

<NSAI> <HAR>

NEMKO <HAR>

<UNDE> <HAR>

ELOT <HAR>

l np l <HAR>

SEV <HAR>

SETI <HAR>

10 30 10

30 10 10

30 30 10

10 30 50

10 10 30

10 30 30

10 10 50

30 10 50

30 10 30

30 30 50

10 10 70

30 10 70

30 30 70

10 10 90

10 30 90

10 30 90

Underwriters Laboratories Inc. (UL) SV, SVT, SJ or SJT, 3 X 18AWGCanadian Standards Association (CSA) SV, SVT, SJ or SJT, 3 X 18AWG

This device complies with the requirements of both directive 89/336/EEC concerning electromagnetic compatibilityand directive 73/23/EEC concerning low voltage. The CE marking indicates compliance with the above directives.

This publication is printed on recycled paper.

Printed in Japan 2000 08 M 005–@

2-43-2,Hatagaya, Shibuya-ku, Tokyo, Japan

Postfach 10 49 08, 20034, Hamburg, Germany

2 Corporate Center Drive, Melville, NY 11747-3157, U.S.A.

491B River Valley Road, #12-01/04 Valley Point Office Tower, Singapore 248373

2-8 Honduras Street, London EC1Y OTX, United Kingdom.

104 Ferntree Gully Road, Oakleigh, Victoria, 3166, Australia