bugs’n’mud e. coli, turbidity and flow relationships for the motueka river lucy mckergow and rob...

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Bugs’n’mud E. coli, turbidity and flow relationships for the Motueka River Lucy McKergow and Rob Davies- Colley

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Page 1: Bugs’n’mud E. coli, turbidity and flow relationships for the Motueka River Lucy McKergow and Rob Davies-Colley

Bugs’n’mud

E. coli, turbidity and flow relationships for the Motueka

River

Lucy McKergow and Rob Davies-Colley

Page 2: Bugs’n’mud E. coli, turbidity and flow relationships for the Motueka River Lucy McKergow and Rob Davies-Colley

Outline

• background• research questions• methods• results• conclusions

Page 3: Bugs’n’mud E. coli, turbidity and flow relationships for the Motueka River Lucy McKergow and Rob Davies-Colley

Background

• E. coli bacteria– indicator for freshwater recreation – source= faecal contamination from warm-

blooded animals– transport = surface runoff, subsurface flows,

direct deposition, re-entrainment of bed sediment

• MfE & MoH (2003) guidelines– <260 cfu/100ml acceptable

• in small streams turbidity can be used as a surrogate for E. coli

Page 4: Bugs’n’mud E. coli, turbidity and flow relationships for the Motueka River Lucy McKergow and Rob Davies-Colley

Research questions

• can turbidity be used as a surrogate for E. coli in large rivers?

• how many E. coli are exported to Tasman Bay?

Page 5: Bugs’n’mud E. coli, turbidity and flow relationships for the Motueka River Lucy McKergow and Rob Davies-Colley

Motueka River

• At Woodmans Bend– 2047 km2

catchment– native + exotic

forest 60%, pasture 20%

– mean flow 82 m3/s– median flow 47

m3/s

Page 6: Bugs’n’mud E. coli, turbidity and flow relationships for the Motueka River Lucy McKergow and Rob Davies-Colley

Dataset

• flood event samples – June 03-June 04– sample interval 10 to 30 minutes –auto

sampler– continuous turbidity - OBS– lab turbidity – NTU– E. coli – Colilert, most probable number/100

mL

• monthly sampling – May 03 – Dec 05

Page 7: Bugs’n’mud E. coli, turbidity and flow relationships for the Motueka River Lucy McKergow and Rob Davies-Colley

1

10

100

1000

10000

1May03 9Aug03 17Nov03 25Feb04 4Jun04 12Sep04 21Dec04

Date

E.

coli

(M

PN

/100

ml)

0

200

400

600

800

1000

1200

Flo

w (

cum

ecs)

event

monthly

flow

Monitoring period

event

monthly

flow

Page 8: Bugs’n’mud E. coli, turbidity and flow relationships for the Motueka River Lucy McKergow and Rob Davies-Colley

Concentrations

baseflow rising falling

E.

coli

(MP

N/1

00

ml)

100

101

102

103

104

105

• concentrations high during events – particularly on rising limbs of hydrographs

Kolmogorov-Smirnov p=0.000

Page 9: Bugs’n’mud E. coli, turbidity and flow relationships for the Motueka River Lucy McKergow and Rob Davies-Colley

E. coli vs flow

1

10

100

1000

10000

1 10 100 1000 10000Flow (m3/s)

E.

coli

(MP

N/1

00 m

l)

baseflowrisingfalling

Page 10: Bugs’n’mud E. coli, turbidity and flow relationships for the Motueka River Lucy McKergow and Rob Davies-Colley

E. coli vs turbidity

1

10

100

1000

10000

1 10 100 1000Lab turbidity (NTU)

E.

coli

(MP

N/1

00 m

l)

baseflowrisingfalling

Page 11: Bugs’n’mud E. coli, turbidity and flow relationships for the Motueka River Lucy McKergow and Rob Davies-Colley

0

1000

2000

3000

4000

5000

18Sep03 12:00 19Sep03 12:00 20Sep03 12:00 21Sep03 12:00 22Sep03 12:00

Date

E.

coli

(M

PN

/100

ml)

0

100

200

300

400

500

600

700

800

900

1000

Flo

w (

m3 /s

) a

nd t

urbi

dity

(N

TU

)

E. coli Flow Field turbidity (NTU)

18-22 Sep 03

E. coli

Turbidity

Flow

Page 12: Bugs’n’mud E. coli, turbidity and flow relationships for the Motueka River Lucy McKergow and Rob Davies-Colley

0

1000

2000

3000

4000

5000

6000

7000

8000

9000

18Jun04 19Jun04 20Jun04 21Jun04 22Jun04 23Jun04 24Jun04 25Jun04 26Jun04

Date

E.

coli

(MP

N/1

00 m

l)

0

200

400

600

800

1000

1200

Flo

w (

m3 /s

) an

d tu

rbid

ity (

NT

U)

E. coli Flow Field turbidity (NTU)

18-21 June 2004

Turbidity

Flow

E. coli

Page 13: Bugs’n’mud E. coli, turbidity and flow relationships for the Motueka River Lucy McKergow and Rob Davies-Colley

Loads

• LOADEST – USGS model– log-linear regression– lnQ, lnQ2, seasonality, decimal time

(centred to eliminate collinearity)

Page 14: Bugs’n’mud E. coli, turbidity and flow relationships for the Motueka River Lucy McKergow and Rob Davies-Colley

LOADEST

• E = 0.55• r2 = 0.69

• mean Ld = 1.4 x 107

#/day

• max Ld = 9 x 108 #/day

Inst loadobs

Daily loadpred

Page 15: Bugs’n’mud E. coli, turbidity and flow relationships for the Motueka River Lucy McKergow and Rob Davies-Colley

Conclusions

• bugs and mud are from different sources

• turbidity may not be a consistently useful surrogate for E. coli in large rivers– alternative is to use flow