bt30 instructions for use biological indicator for dry heat sterilization
TRANSCRIPT
![Page 1: BT30 Instructions for use Biological indicator for Dry Heat sterilization](https://reader038.vdocuments.us/reader038/viewer/2022102611/56649f045503460f94c17798/html5/thumbnails/1.jpg)
BT30 Instructions for useBiological indicator for Dry Heat sterilization
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TUBE B
TUBE A
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Identify TUBE A writing the sterilizer Nº, load Nº and processing date on the indicator label.
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Pack the biological indicator along with the material for sterilization.
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Do not sterilize TUBE B.
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Sterilize as usual.
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Once the sterilization processhas finished, verify that IB labelcolor has changed to brown.Let the IB cool down.
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Break TUBE B ampoule with the crusher in BIONOVA® IC10/20 incubator or with an ampoule crusher.
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Remove and discard the cap of TUBE B under sterile environment or laminar flow.
STERILITYconditions
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Remove the cap of TUBE Aand discard the tube.
STERILITYconditions
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Put TUBE A cap inside TUBE Bin such a way that the sporespaper carrier gets in contactwith the culture medium.IMPORTANT! Make sure thecarrier does not get in contactwith the exterior side of thetube or any other surface.
STERILITYconditions
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Close the cap tightly.
STERILITYconditions
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Place the processed IB in the incubator.IMPORTANT! Use a non-sterilized biological indicator as a positive control each time aprocessed indicator is incubated.
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Incubate at 37±2 ºC for 48 hs. maximum.
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If after incubation the color of the growth indicator medium has not changed, the result is NEGATIVE, thus, the sterilization process is EFFICIENT.
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If after incubation the color of the growth indicator medium has changed to yellow,the result is POSITIVE, thus, the sterilization process HAS FAILED.
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Record the results and discard the tube immediately according to your country's healthcare and safety regulations.
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