bt-m.tech-1-–-4-sem-2015-16

1

M. S. RAMAIAH INSTITUTE OF TECHNOLOGY

(Autonomous Institute, Affiliated to VTU)

BANGALORE-560 054

SYLLABUS

(For the Academic year 2015 2016)

I IV Semester M. Tech

Master of Technology

(Biotechnology)

2

About the Institution: M. S. Ramaiah Institute of Technology (MSRIT) was started in 1962 by the late Dr. M.S. Ramaiah,

our Founder Chairman who was a renowned visionary, philanthropist, and a pioneer in creating

several landmark infrastructure projects in India. Noticing the shortage of talented engineering

professionals required to build a modern India, Dr. M.S. Ramaiah envisioned MSRIT as an institute of

excellence imparting quality and affordable education. Part of Gokula Education Foundation, MSRIT

has grown over the years with significant contributions from various professionals in different

capacities, ably led by Dr. M.S. Ramaiah himself, whose personal commitment has seen the institution

through its formative years. Today, MSRIT stands tall as one of Indias finest names in Engineering Education and has produced around 35,000 engineering professionals who occupy responsible

positions across the globe.

About the Department:

Established in 2002 the department offers a four year BE Biotechnology Program (60 intake) and two

years PG Program, M-Tech in Biotechnology (18 intake). Biotechnology Department has nine well

equipped UG laboratories and one PG laboratory, four lecture halls and one Bioinformatics laboratory

dedicated to biotechnology students alone. It is recognized as a Research Center by Visvesvaraya

Technological University, Belgaum, where students for MS and Ph.D. by research can register. The

department has 14 well qualified faculty members (9 Ph.D and 5 MSc/M-Tech) carrying out research

in various areas of Biotechnology funded by DST, VTU, VGST, RGUHS and KSCST.

3

ORGANIZATION CHART

Dr. NVR Naidu

Principal

Dr. T.V. Suresh Kumar

Registrar (Academics)

Sri. Ramesh Naik

Registrar (Administration)

FACULTY

Faculty Name Qualification Designation Specialization

Dr. Channarayappa MSc (Agri),

Ph.D.(India), Ph.D.

(USA), PDF (USA),

PGDBA

Professor &

Head of the

Department

Biotechnology,

Molecular biology

interdisciplinary approach,

Agriculture

Dr. Bindu S MSc, Ph.D., PDF

(USA)

Associate

Professor

Food Biotechnology

Toxicology

Dr. Chandraprabha MN MSc (Engg.), Ph.D.

(Engg.)

Associate

Professor

Biochemical Engg.

Environmental BT

Dr. Dhamodhar P M.Sc, M.Phil., Ph.D. Associate

Professor

Immunotechnology

Biochemistry

Mr. Lokesh KN M. Pharm (Ph.D.) Assistant

Professor

Pharmaceutical Biotechnology

Dr. Ahalya N MSc, M.Phil, Ph.D.,

PDF

Associate

Professor

Microbiology

Environmental Biotechnology

Dr. Sharath R MSc, Ph.D., Assistant

Professor

Plant & Animal BT

Phytochemistry &

Pharmacology

Dr. Ravi Kumar YS MSc, Ph.D., PDF Assistant

Professor

Cancer Biology

Virology

Dr. Prabha M MSc, Ph.D., PDF Assistant

Professor

Medical biotechnology and

rDNA Technology

Dr. Sravanti V M.Sc., Ph.D. Assistant

Professor

Genomics & Proteomics,

Structural Biology

Mrs. Samrat K M. Tech Assistant

Professor

Nano-Biotechnology,

Microbial Biotechnology

Mr. Gokulakrishna M M. Tech Assistant

Professor

Bio process Engineering

Ms. Bhavya SG M. Tech Assistant

Professor

Enzyme Technology

Downstream processing

Mr. T P Krishna Murthy M. Tech Assistant

Professor

Bioinformatics, Biochemical

and Bioprocess Engineering

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Dr. Pradeepa K M.Sc., Ph.D. Assistant

Professor

Plant biotechnology

Phytochemistry-Pharmaco

5

Vision of the Institute is:

To Evolve into an autonomous institution of international standing for imparting quality technical

education

Mission of the institute

MSRIT shall deliver global quality technical education by nurturing a conducive learning

environment for a better tomorrow through continuous improvement and customization

Vision of the Department is:

To be a leading Biotechnology Engineering department that imparts quality technical education

with strong research component, to develop solutions in the field of food, health and

environment.

The mission of the Biotechnology department is:

To provide quality technical education in a conducive learning environment to produce

professionals, researchers with a zeal for lifelong learning and a commitment to society.

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Process of deriving the vision and mission of the department

Process of Deriving the PEOs of the programme

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Programme Educational Objectives (PEOs) of the program

PEO 1: To impart strong foundation in mathematics, basic and engineering sciences contributing to

Biotechnology.

PEO 2: To produce graduates who can pursue higher education and research in biotechnology and

allied fields.

PEO 3: To produce graduates with an ability to design, develop and implement research projects and

apply to solve problems related to areas of biotechnology.

PEO 4: To provide opportunities to students to work in multidisciplinary teams with professional

ethics, good communication, leadership skills and commitment to society.

Process of deriving the Programme Outcomes

The Programme outcomes are defined taking into account the feedback received from faculty, alumni,

Industry and also from guidelines put across by regulatory/professional bodies and graduate attributes

which are in line with programme educational objectives. The following Figure indicates the

information flow.

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PROGRAM OUTCOMES

By the time of Post graduation in Biotechnology Engineering, students should be able to:

a. Imbibe the essential concepts of both engineering and life sciences &apply it to a wide range of interdisciplinary work.

b. Understand the engineering design, conduct experiments in biotechnology and apply in the field by generating innovative, economical and feasible solutions.

c. Design and automate the processes and programs to accelerate the output for wide applications d. Perform and formulate both concept and empirical based equations and formulas in

biotechnology to solve the problems and to draw meaningful conclusions.

e. Update the modern techniques, skills and advanced engineering tools essential for applications in biotechnology.

f. Mould the students behavior, attitude and interpersonal skills to function in multi-disciplinary teams and setups.

g. Become a responsible citizen by being aware of his/her roles, duties, professional and ethical responsibilities and rights.

h. Develop soft-skills through classroom seminars, institutional and industry interactions, use of modern research and teaching aids

i. Encourage students to take courses from other branches of engineering to have broad-based education and multidisciplinary approach in a global and societal context

j. Create enthusiasm in the candidate for life-long learning and urge to contribute to technology and society by working in a need-based and problem solving projects.

k. Possess knowledge of contemporary issues for sharpening managerial and entrepreneurial skills to commercialize the technology & capture the markets for innovations.

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PROGRAM OUTCOME ASSESSMENT

The matrix given below describes assessment of program outcomes defined above against the

outcome ak as described by ABET

No Program outcome a b c d e f g h i j k

a Imbibe the essential concepts of both engineering

and life sciences and apply it to a wide range of

interdisciplinary work.

H H H H M L

b Understand the engineering design, conduct

experiments in biotechnology and apply in the field

by generating innovative, economical and feasible

solutions.

L H H M M M

c Design and automate the processes and programs to

accelerate the output for wide applications

M H H M L L

D Perform and formulate both concept and empirical

based equations and formulas in biotechnology to

solve the problems and to draw meaningful

conclusions.

L H H H L L

E Update the modern techniques, skills and advanced

engineering tools essential for applications in

biotechnology.

L M H M L L L M

f Mould the students behavior, attitude and interpersonal skills to function in multi-disciplinary

teams and setups.

H M LH L

g Become a responsible citizen by being aware of

his/her roles, duties, professional and ethical

responsibilities and rights

L M H L L L

h Develop soft-skills through classroom seminars,

institutional and industry interactions, use of modern

research and teaching aids

L L L H H L

i Encourage students to take courses from other

branches of engineering to have broad-based

education and multidisciplinary approach in a global

and societal context

L L M H H L L

j Create enthusiasm in the candidate for life-long

learning and urge to contribute to technology and

society by working in a need-based and problem

solving projects.

M L H H L H

k Possess knowledge of contemporary issues for

sharpening managerial and entrepreneurial skills to

commercialize the technology & capture the markets

for innovations.

L M H M H H

Applicable: M, high; M, medium and L, low

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SALIENT FEATURES OF THE PROGRAM First and second semester will focus more on course work in addition research work. The details of course work will be determined by the research guide and research advisory

committee to meet the requirements of the research project.

Compulsory seminar component has been introduced to prepare students for scientific data acquisition, interpretation and presentation to develop professional skills.

Postgraduate students will be assessed periodically based on the internal tests, assignments, practicals, and final examination and research presentations.

Students will be allowed to select their own research project with the approval of their guide(s) and advisory committee.

It is mandatory for PG students to write dissertation, publication of papers and presentation of research papers in the national and international conferences.

CONTINUAL QUALITY IMPROVEMENT

Program outcomes and course learning outcomes will be continuously assessed and evaluated

by faculty, subject experts, and students

giving pertinent information to faculty (lecturers) and Board of studies/examiners

on the effectiveness of the design, delivery, and direction of an educational program.

Improvements base d on periodic re view meeting outcomes and deliberations during BOS/BOE will

close the system loop and the process will continue towards quality improvement

FOCUSEDEDUCATION

Learningoutcomes

GoodGovernance

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37%

26%

7%

30%

CREDIT DISTRIBUTIONTotal number of credits required to Graduate: 100

Core courses Electives Seminars Research work

Course structure:

Breakdown of credits for the M. Tech Degree Curriculum

Semester Core

Courses

Electives Seminars Lab Research

work

Total

I 17 04 01 4 - 26

II 13 08 01 4 - 26

III 04 12 02 - 8 26

IV - - 02 - 20 22

Total 34 24 06 8 28 100

Credit distribution Based on L:T:P:S

Semester Credits*

L T P S Total

I 15 6 4 1 26

II 15 6 4 1 26

III 13 5 8 0 26

IV 0 2 20 0 22

Total 43 21 36 2 100

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Different stakeholders and their interactions contributed for establishment of outcome based

education.

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Board of Studies for the Term 2015-2016

1. Head of the Department concerned (Chairman, BOS):

2. At least four faculty members at different levels covering different specializations constituting

nominated by the Academic Council

3. Special invitees:

1) Two experts in the subject from outside the college

2) One expert from outside the college, nominated by the Vice Chancellor

3) Two representative from industry/corporate sector allied area relating to placement

nominated by the Academic Council

4) One postgraduate meritorious alumnus to be nominated by the Principal

Members of Board of Studies for the year 2015-16

Sl.

No.

Name Institution & Address Chairman /

Member

1 Dr. Channarayappa

Prof. & Head, Dept. of Biotechnology, MSRIT,

Bangalore. Mob: 9449425111

Chairman

2 Dr. Shivaprakash MK Professor and Head, Department of Microbiology,

University of Agricultural Sciences, GKVK Campus,

Bengaluru 560065

Mob: 9845838359

Member

3 Dr. BS Gowrishankar Prof. & Head , Dept. of Biotechnology, SIT, Tumkur

Mob: 9844461940

Member

4 Dr. Nagendra HG Professor and Head of Biotechnology, Sir M

Visvesvaraya Institute of Technology,

Krishnadevaraya Nagar, Hunsamarnahalli, via

Yelahanka, Bangalore-562157

Mob: 9916303565

Member

(VTU

Nominee)

5 Dr. Venkata Ranganna Lead Scientist, M/s. Connexious Life Sciences Pvt.

Ltd.,Bangalore-78,Mob: 9844117742

Email: [email protected]

Member

(Industry)

6 Dr. Manjunath Ramarao Group Director and Head, Biocon-Bristol-Myers

Squibb India Ltd. Biocon-Park, IV Phase

Bommasandra, Bangalore-560099

Mob: 9845238679

email:[email protected]

Member

(Industry)

7 Dr. Bindu S Associate Professor, Dept. of Biotechnology, MSRIT,

Bangalore. Mob: 9448704641

Member

8 Dr. Chandra Prabha MN Associate Professor, Dept. of Biotechnology, MSRIT,

Bangalore, Mob: 9845785174

Alumnus

Member

9 Dr. P Dhamodhar

Associate Professor, Dept. of Biotechnology, MSRIT,


Member

10 Dr. Sharath R Asst. Professor, Dept. of Biotechnology, MSRIT,


Member

14

M-Tech in Biotechnology

SCHEME OF TEACHING FOR THE ACADEMIC YEAR 2013-2014

I SEMESTER M.Tech BIOTECHNOLOGY

Sl. No. Subject

Code

Subject Credits*

L T P S Total

1 MBT 101 Cell Biology & metabolic Engg. 3 1 0 1 5

2 MBT 102 Recombinant DNA Technology 3 1 0 0 4

3 MBT 103 Advanced upstream & downstream

technology

3 1 0 0 4

4 MBT 104 Industrial & Environ. Biotechnology 3 1 0 0 4

5 MBTE Elective-1 4 0 0 0 4

6 MBT 106 Seminar-I 0 1 0 0 1

7 MBT 107L Practical -I 0 0 2 0 2

8 MBT 108L Practical -II 0 0 2 0 2

Total 16 5 4 1 26

II SEMESTER M.Tech BIOTECHNOLOGY

Sl. No. Subject

Code

Subject Credits*

L T P S Total

1 MBT 201 Bioprocess Engineering 3 1 0 1 5

2 MBT 202 Biotechnology of Alternative Fuels 3 1 0 0 4

3 MBT 203 Biopharmaceutical Technology 3 1 0 0 4



6 MBT 206 Seminar-II 0 1 0 0 1

7 MBT 207L Practical III 0 0 2 0 2

8 MBT 208L Practical -IV 0 0 2 0 2

Total 17 4 4 1 26

III SEMESTER M.Tech BIOTECHNOLOGY

Sl. No. Subject

Code

Subject Credits*

L T P S Total

1 MBT 301 Bioethics & Intellectual property rights 3 1 0 0 4




5 MBT 305 *Project work 0 0 8 0 8

6 MBT 306 Seminar-III (evaluation of research phase -I) 0 2 0 0 2

Total 15 3 8 0 26

IV SEMESTER M.Tech BIOTECHNOLOGY

Sl. No. Subject

Code

Subject Credits*

L T P S Total

1 MBT 405 *Project work 0 0 20 0 20

2 MBT 406 Seminar-IV (evaluation of research -phase II) 0 2 0 0 2

Total 0 2 20 0 22

*Project work includes: Laboratory work, Field studies, and other institutional/industrial visits.

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DEPARTMENTAL ELECTIVES M.Tech BIOTECHNOLOGY

Sl.

No.

Subject

Code

Subject Credits

L T P S Total

1 MBTE01 Nanobiotechnology 4 0 0 0 4

2 MBTE02 Genetic Engineering of Value Added Foods 4 0 0 0 4

3 MBTE03 Medical Biotechnology 4 0 0 0 4

4 MBTE04 Bioreaction Engineering 4 0 0 0 4

5 MBTE05 Toxicology and Forensic science 4 0 0 0 4

6 MBTE06 Plant Biotechnology 4 0 0 0 4

7 MBTE07 Experimental Designs 4 0 0 0 4

8 MBTE08 Applied Bioinformatics 4 0 0 0 4

9 MBTE09 Advanced Research Methodology 4 0 0 0 4

10 MBTE10 Applied Animal Biotechnology 4 0 0 0 4

11 MBTE11 Bioanalytical and Biophysical Techniques 4 0 0 0 4

12 MBTE12 Protein Engineering And Industrial Applications 4 0 0 0 4

13 MBTE13 Bioreactor Technology 4 0 0 0 4

*L, Lecture; T, Tutorial; P, Practical; S, Self studies.

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CELL BIOLOGY AND METABOLIC ENGINEERING

Sub Code

Credit

: MBT 101

: 3:1:0:1

CIE

SEE

: 50 Marks

: 50 Marks

Course coordinators: Dr Bindu S. and Dr. Ravikumar Y.S.

Objectives of the course: The course will help to:

1. Learn the fundaments of cell biology and understand the structures and purposes of basic components of cells, especially macromolecules, membranes, and organelles

2. Understand how these cellular components are used to generate and utilize energy 3. Gain a conceptual understanding of the molecular basis of various cellular processes 4. Design effective metabolic engineering strategies with available molecular biology tools

UNIT 1

Cell biology: Cell structure: prokaryotic and eukaryotic cells, Cytoskeleton, Structure and functions of

nucleus, mitochondria, ribosome, Golgi bodies, lysosomes, endoplasmic reticulum, peroxisomes,

chloroplasts, vacuoles, plasma membrane. Fluid mosaic theory of plasma membrane. Active and

passive transport systems of plasma membrane. Cell division and cell cycle regulation (mitosis and

meiosis). Cellular and sensory communications.

UNIT 2

Genetics and inheritance: Introduction to Mendelian genetics, Gene interactions and exceptions to

Mendelian laws. Different types of gene interactions. Structure and organization of genetic material in

eukaryotes, structure of chromosomes, special chromosomes. Linkage and recombination.

Introduction to microbial genetics.

UNIT 3

Plants: Photosynthesis: light and dark reaction, CO2 fixation, respiration and photorespiration,

electron transport chain and ATP synthesis in mitochondria.

Animals: Introduction to human digestive, circulation, respiration, excretion and reproductive

systems. Nervous systems: peripheral and CNS. Introduction to immune system: innate and adaptive

immunity, antibody structure and functions,

UNIT 4

Introduction to metabolic engineering: review of cellular metabolism, models for cellular reactions,

material balance and data consistency- Block box model, elemental balance, heat balance and analysis.

Regulation of enzyme activities and concentration. Regulation of transcription and translation.

Regulation of metabolic networks.

UNIT 5

Manipulation of metabolic pathways: enhancement of product yield and productivity-ethanol, amino

acids and solvents. Product spectrum and novel products: antibiotics, polyketides, vitamins and

biological pigments. Metabolic flux analysis: methods for determination of metabolic fluxes by

isotope labeling-fractional label enrichment. Application of metabolic flux analysis; amino acid

production by Glutamic acid bacteria and mammalian cell cultures, flux analysis of metabolic

networks- bottom-up and top-down approach.

Textbooks:

1. Channarayappa (2010) Cell biology, Universities Press (India Pvt Ltd., Hyderabad.

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2. Hardin J et al. (2012)Beckers World of the Cell VIII edn. Pearson Benjamin Cummings, San Francisco, USA

3. Gregory N. Stephanopounlos, Aritstos A. Aristidou and Jens Nielsen (1998) Metabolic Engineering: principles and methodologies. Academic Press, USA.

Reference Books

1. Shuler Ml and Kargi F (2010) Bioprocess engineering basic concepts, 2nd Edn. Prentice Hall. 2. Cortassa S, Aon MA, Lglesias AA and Lyod L (2002) An introduction and metabolic and

cellular Engineering. World Scientific Publications Pvt Ltd. Singapore.

3. Sujit K Chaudhuri (2010) Concise Medical Physiology, New Central Book Agency, Pvt. Ltd. 4. Pierce BA. (2012), Genetics A Conceptual Approach IV edn. W. H. Freeman and Company

New York

5. Bruce A. et al (2007) Molecular Biology of the Cell, 5th edition, Garland science, New York,USA.

6. Nestor V. Torres and Eberhard O. Voit (2002) Pathways and optimization in metabolic Cambridge University Press.

Course Delivery: Regular black Board teaching and interaction through tutorial class

Assessment and Evaluation Vis--vis Course outcome

What To

whom

When/ Where

(Frequency in

the course)

Max

marks

Evidence

collected

Contributing

to Course

Outcomes

Dir

ect

Ass

essm

ent

Met

hod

s

CIE

Internal

assessment

tests

Students

Thrice(Average

of the best two

will be

computed)

30 Blue books 1,2,3&4

Class-room

open book

assignment

Twice( Average

of the two will

be computed)

10 Assignment

reports 1,2,3&4

Surprise

Test Once 10

Quiz

answers 1,2,3&4

SE

E

Standard

examination

End of course

(Answering 5 of

10 questions)

100 Answer

scripts 1,2,3&4

Ind

irec

t

Ass

essm

ent

Met

hod

s Students feedback

Students

Middle of the

course -

Feedback

forms

1,2,3&4,

delivery of the

course

End of course

survey End of course -

Question-

naire

1,2,3&4,

assessment

methods

Questions for CIE and SEE will be designed to evaluate the various educational

components

S.No Blooms Category Test 1 Test 2 Test 3 Semester-End Examination

1 Remember 25 20 20 25

2 Understand 35 30 30 30

3 Apply 25 30 30 30

4 Analyze 15 15 10 10

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5 Evaluate 0 5 10 5

6 Create 0 0 0 0

Course Outcome: On completion of this course student will have improved ability:-

1. To describe basic biological concepts and principles. 2. To appreciate the different levels of biological organization. 3. To understand that biology has a chemical, physical, and mathematical basis and to explain the

importance of the scientific method to understand natural phenomena.

4. To integrate modern biology with engineering principles

Mapping of course outcome with program outcomes

Course

Outcomes

Program Outcome

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO

11

PO

12

1. X

2. X

3. X X X

4. X X X

RECOMBINANT DNA TECHNOLOGY

Sub Code

Credits

: MBT 102

: 3:1:0:0

CIE

SEE

: 50 Marks

: 50 Marks

Course coordinators: DR. Channarayappa and DR. Prabha M.

Objectives of the course: The course will provide to:

1. Study the mechanism of central dogma and advanced applications of molecular biology. 2. Study the role of the genes, enzymes, proteins and their modifications by genetic engineering

techniques.

3. Construct the transgenic plants and animals for research, Diagnosis, Medicine and Health. 4. Know the advanced applications of recombinant DNA technology in industry, Food,

agriculture and environment.

Syllabus

UNIT-1

Introduction to Recombinant DNA technology: Introduction to recombinant DNA technology. The

importance of recombinant DNA technology. Vectors: Structure and function of cloning and

expression vectors. Regulatory sequences of prokaryotic and eukaryotic genes. Different host systems

for cloning and expression. Transformation techniques: physical, chemical and biological.

UNIT-2

Important enzymes used in the recombinant DNA technology: Enzymes are the molecular tools.

Enzymes cleave nucleic acids: nucleases, restriction endonucleases, RNases. Enzymes synthesize

nucleic acids: DNA and RNA polymerases, ligases. Enzymes in modification of DNA: DNA

methylases, phosphatases, kinases, topoisomerases. Construction and screening of genomic and cDNA

libraries.

UNIT-3

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Advanced techniques used in recombinant DNA technology: Isolation of DNA and RNA.

Estimation of purity and quantity of nucleic acids, Polymerase chain reaction (PCR),

Autoradiography, DNA sequencing. Detection of DNA, RNA and proteins by Southern blotting,

Northern blotting, western blotting and in situ hybridization techniques. Site-specific mutagenesis,

Gene mapping and Microarrays.

UNIT-4

Applications of recombinant DNA technology in Medicine and Health: Production of specialty

chemicals and proteins: secondary metabolites, phytochemicals. Genetically modified microbes

(Recombinant bacteria) for the production of commercial scale production of proteins and

pharmaceuticals, antibiotics, enzymes, insulin, growth hormones, monoclonal antibodies. Applications

rDNA in diagnosis of pathogens and abnormal genes. Transgenic animals. Transgenic animals for

production of proteins and pharmaceuticals. Genetically modified insect cells for the production of

commercially important bioproducts.

UNIT-5

Applications of recombinant DNA technology in agriculture, industry and environment:

Transgenic plants, Transgenic crops for increased yield, resistance to biotic and abiotic stresses,

clearing oil spills. Application of transgenic plants. Industrial production of specialty chemicals and

proteins: organic molecules and commercially important proteins. Biosafety regulations and

evaluation of genetically modified microorganisms (GMOs), plants and animals.

Text books:

1. Channarayappa (2006) Molecular Biotechnology: Principles and Practices. Universities Press (India) Pvt. Ltd. Worldwide publishing: CRC Press, Taylor and Francis.

2. Russell, David W, Sambrook, Joseph (2001). Molecular cloning: a laboratory manual. Volumes I - III. Cold Spring Harbor laboratory Press, USA.

Reference Books:

1. David S Latchman (1994) From Genetics to Gene Therapy the molecular pathology of human disease by, BIOS scientific publishers, sixth edition.

2. Old RW and Primrose SB (1993) Principles of gene manipulation, an introduction to genetic engineering. Blackwell Scientific Publications.

3. Benjamin Lewis (2008) Genes VIII. Oxford University & Cell Press 4. Channarayappa (2010) Cell Biology. Universities Press (India) Private Limited. 5. Molecular Biology (2015) Universities Press (India) Private Limited.



What To whom When/ Where

(Frequency in

the course)

Max

marks

Evidence

collected

Contributing

to Course

Outcomes

Dir

ect

Ass

essm

ent

Met

hod

s

CIE

Internal

assessment

tests

Students

Thrice(Average

of the best two

will be

computed)


Class-room

open book

assignment

Twice( Average

of the two will

be computed)

10 Assignment

reports 1,2,3&4

Surprise

Test Once 10

Quiz

answers 1,2,3&4

20

SE

E

Standard

examination

End of course

(Answering 5 of

10 questions)

100 Answer

scripts 1,2,3&4

Ind

irec

t

Ass

essm

ent

Met

hod

s Students feedback

Students

Middle of the

course -

Feedback

forms

1,2,3&4,

delivery of the

course

End of course


Question-

naire

1,2,3&4,

assessment

methods


components

SL NO. Blooms Category Semester-End Exam

1 Remember 30

2 Understand 30

3 Apply 20

4 Analyze 25

5 Evaluate 5

6 Create 0


1. To know the importance of recombinant DNA technology and its applications. 2. To engineer the genes to regulate expression and produce biomolecules. 3. To develop genetically modified organisms (GMOs) of microbes, animals and plants that can

produce industrially important compounds.

4. To assess the advantages and disadvantages of recombinant DNA technology and its future applications.


Course Outcomes

Program Outcome

a B c D e f g h i j k

1. X X X X X X X X X

2. X X X X X X X X X

3. X X X X X X X X X X

4. X X X X X X X X X X

ADVANCED UPSTREAM AND DOWNSTREAM PROCESSING

Sub Code

Credit

: MBT 103

: 3:1:0:0

CIE

SEE

: 50 Marks

: 50 Marks

Course coordinators: Dr. Chandraprabha M N and Mr. Lokesh K N


1. Learn the fundaments of upstream and downstream processing 2. Understand the principle, working and application of major unit operations in Bioprocessing of

industrially important products.

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3. Understand strategies for development of novel Bioprocessing protocol by applying the concise principles of upstream and downstream processing.

4. Understand principles of different methods of characterization of biomolecules and formulation strategies in order to enhance shelf life of product.

UNIT 1

Media development and Design of Biological reactors: Introduction, Types of cell culture media,

components of animal origin, inoculum development strategies, Ideal reactors, Reactor dynamics,

Sterilization of reactors, Immobilized biocatalysts, Multiphase Reactors, Animal and plant cell reactor

technology.

UNIT 2

Mammalian and plant cell culture technology: Introduction. Cell line transfection and selection,

Increase in efficiency in selecting a producer cell line, Stability of gene expression, Optimization of

the fermentation process, Bioreactors. Bioreactor consideration for plant cells.

UNIT 3

Cell disruption and protein enrichment operations: Centrifugation; Sedimentation; Flocculation;

Microfiltration; Sonication; Bead mills; Homogenizers; Chemical lysis; Enzymatic lysis. Membrane

based purification: Ultrafiltration; Reverse osmosis; Dialysis; Diafiltration;

Pervaporation;precipitation (Ammonium sulfate solvent). Extraction (solvent aquious two phase,

supercritical)..

UNIT 4

Adsorption and chromatography: size, charge, shape, hydrophobic interactions, Biological affinity;

Process configurations (packed bed, expanded bed, simulated moving beds).Electrophoretic technique,

Electrophoresis.

UNIT 5

Product polishing techniques; Crystallization; Drying. Case studies; product formulation and additives,

freeze drying process.

Text Books:

1. Harris ELV and Angal S (1988) Protein Purification Methods, Ed. IRL Press at Oxford University Press.

2. Belter PA, Cussler EL and Wei-Shou Hu (2001) Bioseparations-Downstream Processing for Biotechnology, Wiley-Interscience Publication.

Reference Books: 1. Michael Butler (2007) Cell Culture and Upstream Processing, T & F informa. 2. James E. Bailey and David F. Ollis (1997) Bioprocess Engineering fundamentals. Mc Graw Hill

Book Publication.

3. Bailey JE and Ollis DF (2010) Biochemical Engineering Fundamentals, 2nd Edn, Mc-Graw Hill, Inc.

4. Scopes RK Berlin (1982) Protein Purification: Principles and Practice, Springer.




(Frequency in

the course)

Max

marks

Evidence

collected

Contributing

to Course

Outcomes

22

Dir

ect

Ass

essm

ent

Met

hod

s

CIE

Internal

assessment

tests

Students

Thrice(Average

of the best two

will be

computed)


Class-room

open book

assignment

Twice( Average

of the two will

be computed)

10 Assignment

reports 1,2,3&4

Surprise

Test Once 10

Quiz

answers 1,2,3&4

SE

E

Standard

examination

End of course

(Answering 5 of

10 questions)

100 Answer

scripts 1,2,3&4

Ind

irec

t

Ass

essm

ent

Met

hod

s Students feedback

Students

Middle of the

course -

Feedback

forms

1,2,3&4,

delivery of the

course

End of course


Question-

naire

1,2,3&4,

assessment

methods


components


1 Remember 30

2 Understand 30

3 Apply 15

4 Analyze 20

5 Evaluate 5

6 Create 0


1. To better understand the applied concepts of upstream and downstream processing. 2. To acquire real-time working knowledge on media formulation, sterilization and optimization

process which are considered as pre-requisites of upstream processing.

3. To execute precise and efficient bioseparation process, which in cost effective and yield high degree of pure substance.

4. To develop novel Bioprocess which is gives high resolution, economical bioproducts.


Course Outcomes Program Outcome

a b c d e f g h i j k

1. X X x X X X X

2. X X X X X X X

3. X X X X X X X

4. X X X X X X X x X

INDUSTRIAL & ENVIRONMENTAL BIOTECHNOLOGY

23

Sub Code

Credit

: MBT 104

: 3:1:0:0

CIE

SEE

: 50 Marks

: 50 Marks

Course coordinators: Mrs. Bhavya S G and Dr. Chandraprabha, M.N.


1. Highlight the importance and methods involved in screening of industrial important

microorganisms.

2. Give an insight to manage metabolic processes at both molecular, cell and engineering level.

3. Provide a knowledge on mechanisms enabling microorganisms to produce useful industrial

products of microbial origin

4. Discuss the concepts of various effluent treatments applied to industrial waste generation.

UNIT-1

Introduction to industrial biotechnology: An introduction to fermentation processes - the range of

fermentation processes. Microorganisms used in industrial microbiological processes - the isolation,

preservation, Screening for Productive Strains and Strain Improvement in Biotechnological Organisms

Manipulation of the genome of industrial organisms. Use of recombinant system for the

improvement of industrial microorganisms, Media and materials required for industrial

microbiological processes - sources, formulation, antifoams and optimization.

UNIT-2

Metabolic Pathways for the Biosynthesis of Industrial Products: The Nature of Metabolic

Pathways, Industrial Microbiological Products as Primary and Secondary Metabolites, Trophophase-

idiophase Relationships in the Production of Secondary Products, Role of Secondary Metabolites in

the Physiology of Organisms, Pathways for the Synthesis of Primary and Secondary Metabolites of

Industrial Importance. Carbon Pathways for the Formation of Some Industrial Products Derived from

Primary Metabolism.

UNIT-3

Production of enzymes, organic acids and solvents: Carbon Pathways for the Formation of Some

Products of Microbial Secondary Metabolism of Industrial Importance. Mechanisms Enabling

Microorganisms to Avoid Overproduction of Primary Metabolic Products Through Enzyme

Regulation, Derangement or Bypassing of Regulatory Mechanisms for the Over-production of Primary

Metabolites, Regulation of Overproduction in Secondary Metabolites. Industrial products produced by

microorganisms - Enzymes (amylase, proteases), organic acids (lactic acid, citric acid, vinegar), Ethyl

alcohol.

UNIT-4

Production of antibiotics and health care products: Mechanisms Enabling Microorganisms to

Avoid Overproduction of Primary Metabolic Products Through Enzyme Regulation, Derangement or

Bypassing of Regulatory Mechanisms for the Over-production of Primary Metabolites, Regulation of

Overproduction in Secondary Metabolites - Production of important antibiotics - penicillin,

Cephalosporins, streptomycin, erythromycin, bacitracin, Other beta-lactam antibiotics and

tetracyclines. Production of Vitamins B12 &. Baker's yeast production and hormones. Microbial

transformation of Steroids and sterols. amino acids (L-lysine, L-glutamic acid) as food supplement.

24

UNIT-5

Environmental Biotechnology: Treatment of Wastes in Industry- Wastes from Major Industries,

Systems for the Treatment of Wastes- Aerobic breakdown of raw waste waters, Treatment of the

Sludge- Anaerobic Breakdown of Sludge. Hazardous waste management - Bioremediation, Biological

detoxification- examples of biotechnological applications for hazardous waste management. Mining

and Metal biotechnology, microbial transformation, accumulation and concentration of metals, metal

leaching, extraction and future prospects.

Textbooks:

1. Modern industrial microbiology and biotechnology (2007) nduka okafor, science publishers.

2. Industrial Microbiology (2002), Prescott and Dunns, AVI Publishing Company Inc.

3. Environmental Biotechnology (2005), Alan Scragg, oxford university press.

Reference Books:

1. Stanbury PE, Whitaker A, and Hall SJ (1999) Principles of Fermentation Technology by,

Butterworth Heineman, Aditya Books (P) Ltd.

2. Wulf Crueger and Anneliese Crueger, (2002) A text book of Industrial Microbiology, Panima

Publishing Corporation.




(Frequency in

the course)

Max

marks

Evidence

collected

Contributing

to Course

Outcomes

Dir

ect

Ass

essm

ent

Met

hod

s

CIE

Internal

assessment

tests

Students

Thrice(Average

of the best two

will be

computed)


Class-room

open book

assignment

Twice( Average

of the two will

be computed)

10 Assignment

reports 1,2,3&4

Surprise

Test Once 10

Quiz

answers 1,2,3&4

SE

E

Standard

examination

End of course

(Answering 5 of

10 questions)

100 Answer

scripts 1,2,3&4

Ind

irec

t

Ass

essm

ent

Met

hod

s

Students

feedback

Students

Middle of the

course -

Feedback

forms

1,2,3&4,

delivery of the

course

End of course


Question-

naire

1,2,3&4,

assessment

methods


components


25

1 Remember 30

2 Understand 30

3 Apply 15

4 Analyze 20

5 Evaluate 5

6 Create 0

Course outcomes: Upon completion of this course, the students will be able to:

1. Describe the methods involved in isolation and screening of potential microorganisms for

production of industrial bioproducts.

2. Analyze the different metabolic pathways and its metabolism for the production of desired

processes.

3. Choose and manage appropriate mechanism of microbiological processes to produce

microbiological products.

4. Apply the principles of waste management to treat waste form the bioprocess industries.



A b c d e f g h i j k

1 X X X X

2 X X X X X

3 X X X X X X X

4 X X X X X X X

PRACTICAL I

Sub Code

Credit

: MBT 107L

: 0:0:2:0

CIE

SEE

: 50 Marks

: 50 Marks

Course coordinators: Dr. Sravanti V., Dr. Ahalya N.

Objectives of the course:

1. To improve experimental skills and experimental design methodologies. 2. To isolate, grow and characterize microbes from various sources. 3. To measure pollution levels in waste waters. 4. To provide training in viral and microbial growth in various environmental conditions.

LAB SESSIONS

LIST OF EXPERIMENTS:

1) Isolation of lypolytic bacteria. 2) Production of Amylase by solid state fermentation. 3) Demostration of lysogeny in E. coli. 4) Measurement of BOD/COD of waste water. 5) Determination of sugar/glucose transport in S. cerevisiae. 6) Production and estimation of citric acid from microbes. 7) Production and estimation of lactic acid from microbes. 8) Determination of growth curve and growth inhibition.

26

9) Cultivation of Anaerobes. 10) Production and estimation of Single Cell Protein. 11) Glucose uptake by Yeast and Other bacteria. 12) Ultraviolet irradiation survival curve. 13) Control of micro organism by physical and chemical factors; effects of pH, osmotic factors. 14) Isolation of Yeast and study of its characters.

Note: At least any 12 experiments must be performed

Text Books:

1. Gerhardt, P., Murray, R.G., Wood, W.A. and Kreig, N.R. (1994) Methods of General and Molecular Bacteriology, Ed. American Society for Microbiology, Washington D.C.

2. P. M. Rhodes and P. F. Stanbury (1997) Applied Microbial Physiology : A Practical Approach, IRL Press

Reference books:

1. Cappuccino J.G, Sherman N (1999) Microbiology: A Laboratory Manual, 4th Edn., Addison-

Wesley International Student.

2. Prescott, Harley and Klein (2008) Laboratory Exercises in Microbiology, 7th Ed Harley, McGraw-

Hill, USA

3. Pollack RA, Walter F, Mondschein W, Modesto R (2004) Laboratory Exercises in Microbiology,

2nd Edn.John Wiley Publication.

Course Delivery: Regular black board teaching and interaction through Laboratory sessions



(Frequency in

the course)

Max

mark

s

Evidence

collected

Contributing

to Course

Outcomes

Dir

ect

Ass

essm

ent

Met

hod

s

CIE

Internal

assessment

tests

Students

Once, End of

course 30 Blue books 1,2,3&4

Lab work

Assesment

Continuous 10 Viva- Oral

questions 1,2,3&4

Continuous 10

Record &

Observation

book

1,2,3

SE

E

Standard

examination End of course 50

Answer

scripts 1,2,3&4

Ind

irec

t

Ass

essm

ent

Met

hod

s

End of course

survey Students End of course -

Question-

naire 1,2,3&4


components

SL NO. Blooms Category IA Semester-End Exam

1 Remember 15 10

27

2 Understand 15 20

3 Apply 20 25

4 Analyze 25 25

5 Evaluate 5 15

6 Create 5

Course Outcome: On completion of this course student will have improved ability:

1. To design, execute, and analyze experiments independently. 2. To apply the microbial isolation and growth technique to novel/unknown microbes. 3. To design experiments to monitor the pollution levels of contaminated water. 4. To redesign the lab experiments to extend them into research projects.



A b c d E f g h i j k

1 X X X

2 X X X

3 X X X

4 X X X

PRACTICAL - II

Sub Code

Credits

: MBT108L

: 0:2:0:0

CIE

SEE

: 50 Marks

: 50 Marks

Course coordinators: DR. Prabha, M. and DR. Channarayappa


1. Study the gene cloning and transformation into bacteria. 2. Learn the establishment of cell culture and cell transfection. 3. Production and characterization of recombinant proteins. 4. Analyze the nucleic acids and recombinant proteins with respective blotting techniques.

LAB SESSIONS


1) Introduction to recombinant DNA technology and good laboratory practices. 2) Cell distruption 3) Protein precipitation by NH4SO4 by solvent methods and its recovery 4) Protein purification by chromatography. 5) Cloning: transfer a gene into plasmid vector and transformation into bacteria. 6) Screening of recombinant bacteria. 7) Establishment of plant/animal cell culture. 8) Transformation of plant/animal cells through vector carrying gene of interest. 9) Screening and characterization of transformed cells. 10) Estimation and Isolation of total DNA from tissues/cells 11) Characterization of DNA by Southern blot 12) Detection of recombinant proteins by Western blotting 13) Amplification of target DNA by PCR

28

14) Isolation and quantification of RNA


Text books:

1. Russell, David W, Sambrook, Joseph (2001). Molecular cloning: a laboratory manual. Volumes I - III. Cold Spring Harbor laboratory Press, USA.

2. Channarayappa (2006) Molecular Biotechnology: Principles and Practices. Universities Press (India) Pvt. Ltd. Worldwide publishing: CRC Press, Taylor and Francis.

3. Harris ELV and Angal S (1988) Protein purification methods, Ed. IRL Press at Oxford University press.

Reference Books:

1. Brondyk W. H. (2009) Chapter 11 selecting an appropriate method for expressing a recombinant protein. Methods in enzymology 463: 131-147.

2. Old RW and Primrose SB (1993) Principles of gene manipulation, an introduction to genetic engineering. Blackwell Scientific Publications.



What To whom When/

Where

(Frequency

in the course)

Max

marks

Evidence

collected

Contributing

to Course

Outcomes

Dir

ect

Ass

essm

ent

Met

hod

s CIE

Internal

assessment

tests

Students

Once 30 Blue books 1,2,3&4

Lab

Assesment Continuous 10 Attendance 1,2,3&4

Record Continuous 10 Record 1,2,3&4

SE

E

Standard


Answer

scripts 1,2,3&4

Ind

irec

t

Ass

essm

en

t M

eth

od

s Students feedback

Students

Middle of the

course -

Feedback

forms 1,2,3&4

End of course


Question-

naire

1,2,3&4 and

assessment

methods


components


1 Remember 20

2 Understand 20

3 Apply 35

4 Analyze 20

5 Evaluate 5

6 Create 0

29


1. To know the importance of cloning to understand the gene transformation to bacteria

2. To understand the benefits of cell culture in the applications of genetic engineering.

3. To gain the knowledge in the production of recombinant proteins in research.

4. To know the characteristics of nucleic acids and recombinant DNA proteins in the applications

of health, medicine, industry, Food, agriculture and environment.




1 X X X X X X X X

2 X X X X X X X X X X

3 X X X X X X X X

4 X X X X X X X X X

30

BIOPROCESS ENGINEERING

Sub Code

Credit

: MBT 201

: 3:1:0:0

CIE

SEE

: 50 Marks

: 50 Marks

Course coordinators: Mrs. Bhavya SG and Dr. Chandraprabha MN


1. To identify the essential concepts of bioprocessing and emphasizes the integration of biology

and chemical engineering.

2. To acquire knowledge on kinetics of microbial growth and biocatalyst.

3. To apply the principles of biochemical engineering in design and scale-up of industrial

bioprocess.

4. To develop sustainable bioprocess by applying principles of process economics.

UNIT-1

Bioprocessing Fundamentals:

Biotechnology and Bio-process engineering-Historical development of bioprocess technology-

Difference in approaches by biologist and engineer-Introduction to Bioproducts- Bioprocess principles

and operations- Outline of a bioprocess and the various unit operations involved in bioprocesses. Steps

in bioprocess development-General material balance equation for steady state (for manufacture of

penicillin and ethanol) -Generalized bioprocess flow sheets: example of penicillin/Bacitracin/ethanol.

Bio-process regulatory constraints.

UNIT-2

Microbial growth and product formulation:

Quantification of cell concentration, Phases of cell growth in bath culture, growth associated and non-

growth associated product formation kinetics, environmental factors affecting growth kinetics. Heat

generation by microbial growth. Structured and unstructured models for microbial growth- Substrate

limited growth-models with growth inhibitors- growth model for filamentous organisms. Microbial

interaction in mixed cultures: Major classes of microbial interactions, microbial participation in the

natural cycles of matter, Industrial utilization of mixed cultures in biological waste water treatment.

UNIT-3

Enzyme kinetics:

Specificities of enzyme catalysis-Mechanistic models for simple enzyme kinetics: Michaelis-Menten

Equation and Briggs-Haldane Equation. Experimental determination of rate parameters for Michaelis-

Menten type kinetics, models for inhibited enzyme kinetics. Effect of pH and Temperature,

Immobilized enzyme systems: Methods of Immobilization, Diffusional limitations in immobilized

enzyme systems. Industrial utilization of enzymes.

UNIT-4

Fermentation Principles:

Fermentation Process-General requirements of fermentation Process; An overview of aerobic and

anaerobic fermentation process and their application in industry. Media Design: Medium requirements

for fermentation process-examples of simple and complex media; Design and usage of commercial

31

media for industrial fermentations, Sterilization: Batch and continuous heat sterilization-sterilization

of Liquid media, Filter sterilization of liquids. Thermal death kinetics. Bioreactors and their modes of

operations.

UNIT-5

Development of sustainable Bioprocesses:

Modeling and Assessment in Process Development- Types of Bioprocess and Biocatalysts, Raw

Materials for industrial bioprocess- Biologics and Biosimilars- Process and fermentation models for

development of processes for bioproducts and biopharmaceuticals Sustainability assessment of

Bioprocess. Process economics of bioproducts.

Text Books:

1. Pauline M Doran., Bioprocess Engineering Principles, 2nd Edition, Academic Press, USA,

2013.

2. Michael L Shuler & Fikret Kargi., Bioprocess Engineering: Basic Concepts., 2nd Edition,

Prentice Hall of India, New Delhi, 2008.

3. Elmar Heinzle, Arno P. Biwer, Charles L. Cooney. Development of Sustainable Bioprocesses

Modeling and Assessment, John Wiley & Sons Ltd, 2006.

Reference Books:

1. James E Bailey & David F Ollis., Biochemical Engineering Fundamentals, 2nd Edition,

McGraw Hill Book Co.-Singapore, 1986.

2. Tapobrata Panda., Bioreactors: Analysis and Design, 1st Edition, Tata McGraw Hill Education

Private Limited, New Delhi, 2011.

3. Douglas S. Clark, Harvey W. Blanch., Biochemical Engineering, 2nd Edition, CRC Press,

1995.



What To

whom

When/ Where

(Frequency in

the course)

Max

marks

Evidence

collected

Contributing

to Course

Outcomes

Dir

ect

Ass

essm

ent

Met

hod

s

CIE

Internal

assessment

tests

Students

Thrice(Average

of the best two

will be

computed)


Class-room

open book

assignment

Twice( Average

of the two will

be computed)

10 Assignment

reports 1,2,3&4

Surprise

Test Once 10

Quiz

answers 1,2,3&4

SEE Standard

examination

End of course

(Answering 5 of

10 questions)

100 Answer

scripts 1,2,3&4

In dir

ect

As

ses

sm ent

M eth

od s Students feedback Students

Middle of the

course -

Feedback

forms

1,2,3&4,

delivery of the

32

course

End of course


Question-

naire

1,2,3&4,

assessment

methods


components


1 Remember 30

2 Understand 30

3 Apply 15

4 Analyze 20

5 Evaluate 5

6 Create 0

Course Outcome: On completion of this course student will have improved ability to:-

1. Describe the steps in a catalytic mechanism and how one goes about deriving a rate law, mechanism, and rate-limiting step that are consistent with biological experimental data.

2. Ability to understand the chemical and physical transport processes and their mechanism in bioreactors.

3. To apply the design procedures of process equipment used in biochemical process plants to design novel bioreactors.

4. To apply and analyze metabolic engineering concepts in industrial bioprocess.


Course Outcomes

Program Outcome


1 X X X X X

2 X X X X X X

3 X X X X X X

4 X X X X X X X

BIOTECHNOLOGY OF ALTERNATIVE FUELS

Sub Code

Credit

: MBT 202

: 3:1:0:0

CIE

SEE

: 50 Marks

: 50 Marks

Course coordinators: Dr. Channrayappa and Dr. Ahalya, N.


1. Know the different sources of energy to use as alternative fuel 2. Understand the principle and procedure for converting raw material into biofuel 3. Develop design and working principle for biodiesel production unit 4. Standardize the biodiesel efficiency and calculate cost benefit ratio of biofuel.

UNIT 1

33

Introduction to energy, sources of energy, renewable energy. non-renewable energy resources.

Alternative biofuels, bio-diesel. List of vegetable oils, biodiesel from vegetable oils and animal fats.

Production of biodiesel. Biofuel plants and their design.

UNIT 2

General aspects of trans-esterification. Factors influencing the transesterification Biodiesel fuel

properties. Characteristics of efficient biodiesel. glycerol - biodiesel byproduct. biodiesel and the

environment. Biodiesel standards. Biofuel policy. Biofuel and biodiesel in India and Biodiesel around

the world.

UNIT 3

Design and development of biofuel production unit. Assessing effect of variation of injection pressure

on performance and exhaust emission of C.I. engine using biodiesel, combustion enrichment and

emission reduction of biodiesel using exhaust gas recirculation and fuel conditioning,

UNIT 4

Effect of air preheating influence on performance and emission characteristics of di diesel engine

using Jatropha curcas, coconut oil, pongamia (karanj) oil, karanji seed (Pongamia glabra) oil-diesel,

esterified mahua oil, tree borne seed oils-engine, diesel-vegetable oil blends and pungam methyl ester.

Ricardo variable compression engine.

UNIT 5

Emission testing of diesel engine on B20 biodiesel blend as per EMA durability test/nitin,

Environmental effects of biodiesel-exhaust emission, establishing auto-gasification of coir pith using

thermo gravimetric analysis. Calculate the biofuel cost benefit ratios for various biofuels.

Textbooks:

1. Meena Devi GS and Nagendra Prasad (2007) Fuels & Biofuels, Vijayalakshmi Agrobios India 2. Poonia MP and Mehla SK (2008) Biofuels Engine Performance & Exhaust Emissions, Pointer

Pub, India

Reference Books:

1. Ahindra Nag (2007) Biofuels Refining and Performance, McGraw-Hill Professional, India 2. Ayhan Demirbas (2008) Biofuels: Securing the Planets Future Energy Needs (Green Energy and

Technology), Springer, Netherland



What To

whom

When/ Where

(Frequency in

the course)

Max

marks

Evidence

collected

Contributing

to Course

Outcomes

Dir

ect

Ass

essm

ent

Met

hod

s

CIE

Internal

assessment

tests

Students

Thrice(Average

of the best two

will be

computed)


Class-room

open book

assignment

Twice( Average

of the two will

be computed)

10 Assignment

reports 1,2,3&4

Surprise

Test Once 10

Quiz

answers 1,2,3&4

34

SE

E

Standard

examination

End of course

(Answering 5

of 10 questions)

100 Answer

scripts 1,2,3&4

Ind

irec

t

Ass

essm

ent

Met

hod

s

Students

feedback

Students

Middle of the

course -

Feedback

forms

1,2,3&4,

delivery of the

course

End of course


Question-

naire

1,2,3&4,

assessment

methods


components

ASSESSMENT PATTERN:

SL NO. Blooms Category End- Semester Examination

1 Remember 25

2 Understand 25

3 Apply 25

4 Analyze 15

5 Evaluate 10

6 Create 0

Outcome of the course: The students will be able to

1. Identify the biofuel source to use as an alternative energy to fossil fuel 2. Standardize the process to convert raw material into biodiesel. 3. He can standardize the designs and improve the biodiesel production efficiency at industrial

scale.

4. Evaluate different biodiesel sources for various parameters to meet the national and international standards and work out economical feasibility of different energy sources.



a b c D e f g h i j K

1 X X X X X X

2 X X X X X

3 X X X X X

4 X X X X X

BIOPHARMACEUTICAL TECHNOLOGY

Sub Code

Credit

: MBT 203

: 3:1:0:0

CIE

SEE

: 50 Marks

: 50 Marks

Course coordinators: Mr. Lokesh, K.N. and Dr. Dhamodhar P.


1. Introduction to pharmaceutical Biotechnology and its applied aspects in drug discovery and development.

35

2. Understand the principle of development of immunotherapeutic and recombinant therapeutic agents.

3. Understanding the influence of nanotechnology in designing and development of bio-therapeutics.

4. Study of strategies for formulation of biotherapeutic products.

UNIT-1

Drug development process of protein based therapeutics: Transforming New Molecular Entities

into Drugs, Differences between Development of Biotechnology Products of Macromolecules and

Chemical Products, Current Trends in Drug Development, Drug designing: Rational, combinatorial

and High Throughput screening.

UNIT-2

Immuno-pharmacology: Overview to immunopharmacology, Antibody-mediated response,

Vaccines, Cell mediated immune response, Cancer immunotherapy, Immunosuppressant and

immunostimulators.

UNIT-3 Biotherapeutics: Hematopoietic Growth Factors and Coagulation Factors, Interferons and Cytokines for Anti-infective and Cancer Therapy, Hormones, Enzymes, Antibodies and Derivatives.

UNIT-4

Nanotechnology for the delivery of proteins and nucleic acids based therapeutics: Introduction to

Nanotechnology in drug deliver, Nano-sized Advanced Delivery Systems as Parenteral formulation

Strategies for Hydrophobic Anti-cancer Drugs, Engineering of Amphiphilic Block Copolymers for

Drug and Gene Delivery, Nanoemulsions for Intravenous Drug Delivery. Nanotechnology for Cancer

Chemotherapy, Nanotechnology for Cancer Vaccine Delivery.

UNIT 5

Formulation of proteins and peptides: Making Small Protein Particles, Lyophilization, Multiphase

Drug Delivery Systems, Protein Compaction, Self-Emulsifying Drug Delivery Systems, skin and

parental drug delivery system.

Text books:

1. Christine M. Bladon (2002) Pharmaceutical Chemistry, John Wiley & Sons, Ltd. 2. Manfred E. Wolff (2000) Burgers Medicinal Chemistry and Drug Discovery (5th edition) A

Wiley & Sons, Inc.

3. Grietje Molema and Dirk KF. Meije (2002) Drug Targeting Organ-Specific Strategies r. Wiley-VCH.

4. Melgardt M. de Villiers (2007) Nanotechnology in Drug Delivery, Springer.

Reference Books: 1. Rodney JY, Milo Gibaldi (2003) Biotechnology and Biopharmaceuticals transforming proteins

and genes into drugs, A John Wiley & Sons, Inc., Publication.

2. Gavin Brooks (1998) Biotechnology in Healthcare, An introduction to biopharmaceuticals, Pharmaceutical Press (London).

3. Shayne cox gad ( 2007) Handbook of pharmaceutical Biotechnology A John Wiley & Sons, Inc., Publication

4. Grietje Molema and Dirk KF (2002) Drug Targeting Organ-Specific Strategies by Meijer. Wiley-VCH.

36


Assess

ment and Evaluation Vis--vis Course outcome


(Frequency in

the course)

Max

marks

Evidence

collected

Contributing

to Course

Outcomes

Dir

ect

Ass

essm

ent

Met

hod

s

CIE

Inte

rnal

asse

ssm

ent

test

s

Students

Thrice(Average

of the best two

will be

computed)


Cla

ss-

room

open

book

assi

gn

men

t Twice( Average

of the two will

be computed)

10 Assignment

reports 1,2,3&4

Surprise

Test Once 10

Quiz

answers 1,2,3&4

SE

E

Standard

examination

End of course

(Answering 5 of

10 questions)

100 Answer

scripts 1,2,3&4

Ind

irec

t

Ass

essm

ent

Met

hod

s

Students

feedback

Students

Middle of the

course -

Feedback

forms

1,2,3&4,

delivery of the

course

End of course


Question-

naire

1,2,3&4,

assessment

methods


components

Course Outcome: On completion of this course student will have improved ability to:

1. To Impart principle aspects of pharmaceutical biotechnology in research and development existing and novel biotherapeutic proteins.

2. Understanding of basic mode of protein based drug formulation and development by understanding the physico-chemical and pharmacological properties of drugs.

3. To apply the recent trends of nanotechnology in production, research- development of nano-medicines.

4. Understanding the pre-requisite basic information required to work in biopharmaceutical or clinical research organization


Course Outcomes

Program Outcome



1 Remember 30

2 Understand 30

3 Apply 15

4 Analyze 20

5 Evaluate 5

6 Create 0

37

1 X X X X x x

2 X X X X X

3 X X X X X X X

4 X X X X X X x x

PRACTICALS - III

Sub Code

Credits

: MBT 207L

: 0:0:2:0

CIE

SEE

: 50 Marks

: 50 Marks

Course coordinators: DR. Channarayappa and Dr. Ravikumar, Y.S.

LAB SESSIONS


1. Study the production of biodiesel from various plant sources 2. Learn the parameters and properties of biodiesel 3. Describe cross-flow filtration with membranes and analyze the adsorption process for samples. 4. Estimate the phytochemicals from plant sources and study the suspension culture


1. Estimation of total oil content of seed

2. Determination of density and Viscosity of oil/biodiesel

3. Estimation of free fatty acids (Acid value) in oil / biodiesel

4. Determination of iodine value of the oil

5. Determination of Saponification value of oil

6. Production of BioDiesel by Conventional Transesterification

7. Cultivation of Oil-Producing algae

8. Production of biodiesel from algae

9. Production of bio-ethanol from various surceases

10. Micro propagation of oil yielding plants

11. Production and Estimation of citric acid

12. Estimation of Lycopene from tomato fruits

13. Estimation of Anthocyanin from leaf /callus tissue

14. Induction of Secondary metabolite Anthocyanin/catheranthin


Reference Books



What To whom When/

Where

(Frequency

in the course)

Max

marks

Evidence

collected

Contributing

to Course

Outcomes

Dir

ect

Ass

essm

ent

Met

hod

s C

IE

Internal

assessment

tests Students


Lab Continuous 10 Attendance 1,2,3&4

38

Assesment


SE

E

Standard


Answer

scripts 1,2,3&4

Ind

irec

t

Ass

essm

en

t M

eth

od

s Students feedback

Students

Middle of the

course -

Feedback

forms 1,2,3&4

End of course


Question-

naire

1,2,3&4 and

assessment

methods


components


1 Remember 20

2 Understand 20

3 Apply 35

4 Analyze 20

5 Evaluate 5

6 Create 0


1. To learn the biodiesel production

2. To understand the parameters of biodiesel and its a

3. To purify the samples with membranes in cross-flow filtration and analysis of the seperation by

adsorption.

4. To quantify the phytochemicals from plant sources and to develop the callus from suspension

culture




1 X X X X X X

2 X X X X X X


4 X X X X X X

PRACTICALS - IV

Sub Code

Credits

: MBT 208L

: 0:0:2:0

CIE

SEE

: 50 Marks

: 50 Marks

Course coordinators: Mr. Lokesh, K N and Dr. Dhamodhar P

LAB SESSIONS


1. Basic biopharmaceutical techniques which are used to standardization and characterization of biopharmaceuticals

39

2. Imparting knowledge of in vitro models to assess biological potency of therapeutic compounds.

3. To learn the techniques of isolation and purification of bioactive compounds from plants


1) Determination of minimum inhibitory concentration of given antibiotic. 2) LAL test for parenteral injection. 3) Bacteriological Test for Water for injection (WFI). 4) Standardization of herbalformulation. 5) Validation of Autoclave. 6) Lyophilization of protein based product. 7) Analysis of multicomponet formulation by spectroscopic method. 8) Bioassay of Vitamins/ Antibiotics. 9) Tri- dot test for HIV 10) Determination of Partition coefficient of given formulation. 11) Determination of antioxidant activity of given formulation. 12) Extraction and isolation of Nutraceuticals from given sample. 13) ELISA techniques (Demo). 14) Preparation of controlled release formulation. 15) Preparation of liposome for drug delivery.


Textbooks:

1. Industrial Pharmaceutical Biotechnology by Heinrich Klefenz, Wiley-VCH edition.,1995 2. Gary Walsh (1998) Biopharmaceuticals: Biochemistry and Biotechnology.

Reference Books: 1. Gregory Bock, Dalia Cohen, Jamie Goode, Novartis and J. Craig Venter (2001) From Genome

to Therapy: Integrating New Technologies with Drug Development - No. 229.

2. Susanna Wu-Pong, Yongyut Rojanasakul, and Joseph Robinson (2006) Biopharmaceutical Drug Design and Development.

3. Herbert A Kirst, Wu-Kuang Yeh, Milton J (2001) Enzyme technologies for pharmaceutical and biotechnological applications



What To whom When/

Where

(Frequency

in the course)

Max

marks

Evidence

collected

Contributing

to Course

Outcomes

Dir

ect

Ass

essm

ent

Met

hod

s CIE

Internal

assessment

tests

Students


Lab

Assesment Continuous 10 Attendance 1,2,3&4


SE

E

Standard


Answer

scripts 1,2,3&4

40

Ind

irec

t

Ass

essm

en

t M

eth

od

s Students feedback

Students

Middle of the

course -

Feedback

forms 1,2,3&4

End of course


Question-

naire

1,2,3&4 and

assessment

methods


components


1 Remember 20

2 Understand 20

3 Apply 35

4 Analyze 20

5 Evaluate 5

6 Create 0


1. Students will be capable to perform important quality control tests to validate quality of

product.

2. Able to standardize the therapeutic compounds.

3. Competent to work in Pharmaceutical /Clinical research organization.

4. Improving the research aptitude of students by imparting the knowledge of nanotechnology

and advanced drug delivery system




1 X X X X X X

2 X X X X X X


4 X X X X X X

41

BIOETHICS & INTELLECTUAL PROPERTY RIGHTS

Sub Code

Credits

: MBT 301

: 3:1:0:0

CIE

SEE

: 50 Marks

: 50 Marks

Course coordinator: Dr Bindu S


1) Instill awareness on ethical issues connected to BT through case studies. 2) Introduce the concept of Biosafety & its significance through case studies. 3) Familiarize students with International as well as national biosafety regulations and case

studies as applied to transgenic research.

4) Make the students conversant with Intellectual property rights and their protection.

UNIT-1

Introduction to Bioethics and Biosafety: definition and needs of Bioethics, Social and Ethical issues

in biotechnology. Application of bioethics: the expanding scope of ethics from biomedical practice to

biotechnology. Introduction to Biosafety: definition and needs of biosafety, levels of biosafety,

applications of biosafety at work place, Biosafety during development of biotech products. Examples

and case studies.

UNIT-2

Ethical Issues: Ethical issues regarding genetically modified organisms (foods and crops); bioethics

in biodiversity and resource management. Animal cloning and human cloning and their ethical

aspects. Testing of drugs on human volunteers, organ transplantation and ethical issues;

Xenotransplantion and its ethical and social issues. Human Genome project.

UNIT-3

Biosafety regulations in transgenic research: National and international guidelines on rDNA

technology. MOEF guidelines, Good laboratory practice, Good manufacturing practice and FDA

regulations, Regulations for recombinant DNA research and manufacturing process, Public perception.

National Institute of health (NIH) guideline, guidelines for research in transgenic organisms.

UNIT-4

Introduction to IPR: IP definition and needs, GATT & WTO, Different forms of IPR Copyrights, Trademarks, Industrial designs, Geographical Indications, Traditional Knowledge, Plant varieties,

Trade Secrets. WIPO, TRIPS, Role of IPR in Research and Development.

Trademarks and copyrights: nature of trademarks and branding, tips on names for trademarks,

acquiring trademarks protection, brand valuation, packaging and selling, increase the value of a

technology through the use of trademark. Introduction and characteristics of copyrights and

neighboring rights, performers and broadcasting organizations rights, transfer of copyrights.

UNIT-5

Patents: Introduction of Patents, patent as an intellectual property, Brief history of patents-Indian and

global scenario, types of patents, patent life cycle, criteria for patenting, novelty, inventiveness, utility,

patentable subject matter, inventions that are not patentable, term of patent, maintenance of a patent,

granted patents Vs. patent publications.

Ideas: Generation and review of ideas, documenting ideas, literature scanning for possibility of IP

rights, decision to go for IP protection or not, and consideration of choice of IP protection, disclosure,

inventors interview, Process and Product Patents.

42

Textbooks:

1. Sateesh M.K (2008) Bioethics & Biosafety, IK Publishers. 2. Traynor PL (2000) Biosafety Management, Virginia polytechnic Institute Publication. 3. N K Acharya (2007) Text book on Intellectual Property Rights, Asia Law house, 4th edn.

Reference Books:

1. Sasson A (1993) Biotechnologies in developing countries present and future, UNESCO Publishers. 2. Rao MB (2003) WTO and International Trade, Vikas Publishing House Pvt. Ltd. 3. Erbisch FH and Maredia KM (2003) Intellectual Property Rights in Agricultural Biotechnology,

Orient Longman Ltd.

4. Deborah E Bouchoux (2005) Intellectual Property Rights, Delmar Cengage learning 5. Patent Fundamentals for Scientists and Engineers, Thomas T Gordon and Arthur S Cookfair, CRC

Press (1995).

Course Delivery: Regular black Board teaching, Using teaching aids like power point presentation

and interaction through tutorial class



(Frequency in

the course)

Max

marks

Evidence

collected

Contributing

to Course

Outcomes

Dir

ect

Ass

essm

ent

Met

hod

s

CIE

Internal

assessment

tests

Students

Thrice(Average

of the best two

will be

computed)


Assignment Once 10 Assignment

reports 1&2

Mini Project

Once 10

Mini Project

reports

3&4

SE

E

Standard

examination

End of course

(Answering 5 of

10 questions)

100 Answer

scripts 1,2,3&4

Ind

irec

t

Ass

essm

ent

Met

hod

s

Students feedback

Students

Middle of the

course -

Feedback

forms 1&2

End of course


Question-

naire 1,2,3&4


components

S.No Blooms Category Test 1 Test 2 Test 3 Semester-End Examination

1 Remember 10 10 10 10

2 Understand 30 30 30 30

43

3 Apply 30 30 30 30

4 Analyze 30 30 30 30

5 Evaluate 0 0 0 0

6 Create 0 0 0 0

Course Outcome: On completion of this course student will be

1) Aware of the ethical issues involved in BT. 2) Capable of understanding biosafety issues in BT 3) Conversant with biosafety regulations & its applications in real life situations connected to

transgenic research.

4) Conversant with the procedures used to protect intellectual property rights.


Course

Outcomes

Program Outcome

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

1 X X X X

2 X X X X

3 X X X X X X

4 X X X X X X X

NANO-BIOTECHNOLOGY

Sub Code

Credits

: MBTE01

: 4:0:0:0

CIE

SEE

: 50 Marks

: 50 Marks

Course coordinator: Dr Ravikumar Y S and Dr. Bindu, S


1) Provide an overview of the fundamental concepts of nanotechnology 2) Develop an understanding of bionanomaterials 3) Understand the applications in the area of Biotechnology especially in health and medical

Biotechnology.

4) Provide an understanding of the benefits & risks of nanotechnology.

UNIT-1

Introduction to Nanotechnology and Nanobiotechnology. History and scope of nano technology;

role of size in nanomaterials: Properties of nano materials- Physical & Chemical properties.

Classification of nano particles- nano-clusters, nanotubes, nanowires and nanodots. Electronic

structure: quantum dots, quantum wires and quantum wells, confinement of electrons energy

quantization, Semiconductor nanocrystals, carbon nanotubes, quantum wells.

UNIT 2 Synthesis of Nanomaterials: Chemical Method: Chemical precipitation and coprecipitation; Metal

nanocrystals by reduction, Sol-gel synthesis; Microemulsions or reverse micelles, myle formation;

Solvothermal synthesis; Thermolysis routes, Microwave heating synthesis; Sonochemical synthesis;

Electrochemical synthesis; , Photochemical synthesis, Synthesis in supercritical fluids. Physical

Methods: Vapor deposition and different types of epitaxial growth techniques- pulsed laser deposition

- Magnetron sputtering - Micro lithography (photolithography, soft lithography, micromachining, e-

44

beam writing, and scanning probe patterning). Biological Methods: Microbial production of inorganic

nanoparticles Magnetosomes .DNA based nanostructures

UNIT-3

Characterization of Nanomaterials : Structural Characterization: X-ray diffraction, Small angle X-

ray Scattering, Optical Microscope and their description, Scanning Electron Microscopy (SEM),

Scanning Probe Microscopy (SPM), , Scanning Tunneling Microscopy (STM), Atomic force

Microscopy (AFM). Spectroscopic characterizations: application of UV-VIS-IR Raman spectroscopy

for analysis of nanomaterials, Surface Characterization: X-ray Photoelectron Spectroscopy (XPS),

Auger electron spectroscopy, Low Energy Ion, Scattering Spectroscopy (LEISS), Secondary Ion Mass

Spectroscopy (SIMS), Rutherford Backscattering Spectroscopy (RBS). Resonance Methods: Electron

Spin Resonance (ESR), Ferromagnetic Resonance (FMR), Nuclear Magnetic Resonance (NMR),

Mossbauer Spectroscopy.

UNIT 4

Biological Nano-materials: Protein based nanostructures building blocks and templates Proteins as transducers and amplifiers of biomolecular recognition events Nanobioelectronic devices and polymer nanocontainers. DNA based nanostructures Topographic and Electrostatic properties of DNA and proteins Hybrid conjugates of gold nanoparticles DNA oligomers Use of DNA molecules in nanomechanics and Computing. Nano diamonds. Biocompatable polymers: liposomes,

dendrimers, chitosan

UNIT 5 Biological Application of Nanotechnology: Nanoparticles in Therapeutic applications Drug delivery, imaging and cancer treatment, bone substitutes and dentistry, Implants and Prosthesis,

Reconstructive Intervention and Surgery, Nanorobotics in Surgery, Photodynamic Therapy, Neuro-

electronic Interfaces, Protein Engineering. Nanotechnology in Agriculture and Food Technology,

Biosensors: Principles- DNA based biosensors Protein based biosensors, Nanosensors in Diagnosis. DNA Templated Electronics, Sequence specific molecular lithography, Single Biomolecule. Manipulation for Bioelectronics, DNA as a semiconductor. Environmental issues, toxicity of

nanomaterials., ethical issues, the future of nanotechnology in medicine.

Text books:

1. Edelstein A.S, Cammaratra R.C (1996) Nanomaterials: Synthesis, Properties and Applications, Second Edition, CRC PressTaylor and Francis group New York USA

2. Christof M. Niemeyer, Chad A. Mirkin (2004)Nanobiotechnology: Concepts, Applications and Perspectives John Wiley & Sons

3. Yubing Xie (2012)The Nanobiotechnology Handbook CRC Press Taylor and Francis group

New York USA

Reference Books:

1. Richard Booker and Earl Boysen (2005) Nanotechnology, Wiley Dreamtech. 2. Chapman & Hall (2002) NanobiotechnologyBasic Science & Emerging Technologies, CRC

Press.

3. Eric K Drexler, Pelerson C, Pergamit G (1993) Unbounding the future. William M

bt-m.tech-1-–-4-sem-2015-16

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