bsc1010c final lab practical review click on enter to see the answer. click enter again to move to...
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BSC1010C Final Lab Practical Review
Click on enter to see the answer.Click enter again to move to next slide.
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What was the substratein the enzyme experiment?
Hydrogen Peroxide (H2O2)
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What was the enzyme in the enzyme experiment?
catalase
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What was the source of thisenzyme in the enzyme lab?
Sheep’s blood
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What was/were the product(s)in the enzyme experiment?
Water and oxygen gas
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What was the optimum pHfor the enzyme?
pH 7
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Why is the enzyme less active at very high or very low pH?
Enzyme is denatured and reaction stops
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What was the optimum temperaturefor the enzyme experiment?
40 C
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Why do you see little gas produced at low temperatures?
Enzyme and substrate molecules are moving very slowly, so less enzyme-substrate collisions,
So less reactions
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Why do you see little or no gas production at high temperatures?
The enzyme, which is a protein, is denaturedat high temperatures and reaction stops.
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What did we measure to determinethe rate of the enzyme reaction?
Oxygen gas pressure – rate of oxygen gas production
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What was the control in the enzyme experiment?
Distilled water in place of enzyme
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What was the inhibitor used in enzyme experiment?
Copper sulfate (CuSO4)
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What should happen when the inhibitor is added in the enzyme experiment?
Reaction should stop as the inhibitor is preventing the substrate and enzyme from coming together
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What happened to the enzyme reaction whenthe temperature was raised to 60 C?
Reaction stopped because enzymewas denatured
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What is this plant?
Coleus
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What is the name of the pigmentin these leaves that is water-soluble?
What color is this pigment?
Anthocyaninred
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What pigment is alcohol-soluble?What color is this pigment?
Chlorophyllgreen
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What was used to indirectly detectphotosynthesis in these leaves?
IKI
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What areas of the leaf carried out photosynthesis according to IKI test?
Green areas (where chlorophyll was located)
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What is the name of the techniquethat uses this setup?
chromatography
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What was this setup used toseparate?
Plant pigments
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What is the genus of plant used to get these pigments from the leaves?
Magnolia
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Name 3 things that determine the movement of the pigments up the paper.
1. Size of pigment molecule (molecular weight)2. Solubility of pigment in the solvent
3. H bonding of pigment to paper
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What causes the solvent tomove up the paper?
Capillary action (adhesion + cohesion)
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What is used to set 100%in absorption spectrum experiment?
acetone
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What is the equation forcell respiration? (balanced, please)
C6H12O6 + 6 O2 6 CO2 + 6 H2O
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What is the balanced equationfor photosynthesis?
6 CO2 + 6 H2O C6H12O6 + 6 O2
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Given cell respiration and photosynthesis,which happens at all times?
Cell respiration
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Given cell respiration and photosynthesis, which happens only in the light?
photosynthesis
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Where is photosynthesis occurringin the following graph?
A and C
(Photosynthesis is taking in CO2 so level falls)
A B C D
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What happens to CO2 levels in the respirometerin the light? WHY?
Decline Because photosynthesis is taking in CO2.
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What happens to CO2 levels in the respirometerin the dark? WHY?
They go up.Photosynthesis is NOT occurring so only cell
respiration which is producing CO2
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What genus of plant was used in the cell respiration – photosynthesis experiment?
Viburnum
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What is the purpose of the beaker of water that was placed between the light source and the leaves?
To act as a heat sink (absorb the heat given off by the light)
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What are the 2 typesof nuclear division?
Mitosis and Meiosis
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In mitosis, how many cells are formed?What is their chromosome #?
2 cells
Diploid number
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In meiosis, how many cells are formed?What is their chromosome #?
4 cells
Haploid #
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What is the name of this region?
Root cap
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What is the name of this region?
Region of maturation or differentiation
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What is the name of this region?
Region ofelongation
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What is the name of this region?
Apical meristemOr
Region of cell division
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What is this stage? Is it a plant or animal cell?
Anaphase
Plant cell
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What stage is this?What is arrow pointing to?
Telophase
Cleavage furrow
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What is this phase?Plant or animal cell?
Prophase
Plant cell
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What is this phase?Plant or animal cell?
Metaphase
Plant cell
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What is this phase?What is arrow pointing to?
Telophase
Cell plate of plant cell
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What is this phase?Plant or animal cell?
Anaphase
Animal cell
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What is this phase of meiosis?
Metaphase I
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What is this phase of meiosis?
Anaphase I
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What is this phase of meiosis?
Telophase I
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What specimen was used to study mitosisin animals?
Whitefish blastula
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What specimen was used to studymitosis in plants?
Onion root tip
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What cell division process occursin somatic cells?
mitosis
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What cell division process producesgametes and spores?
meiosis
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What cell division process is precededby S in the cell cycle?
Both Mitosis and Meiosis I
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How many chromosomes are in the cell below?
2
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What is this phase of meiosis?
Prophase I
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What are the monomers that make up DNA?
nucleotides
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If a piece of DNA has 4 sites recognized by the enzyme, how many fragments will be produced?
5
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Enzymes that cut up DNA are called ___.
Restriction enzymesOr
Restriction endonucleases
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Name an example of these enzymes.
EcoRI
HindIII
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What produces the enzymes that cut up DNA?Why?
Bacteria
To cut up invading phage (bacterial viruses)
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What is the source of the DNA used in this experiment?
Bacteriophage lambda
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What is this piece of equipment?
micropipet
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What is the volume this is set to measure(specify units)?
50 L
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What is the purpose of the 2 “stop points”?
1st Stop – take up the liquid from tube2nd Step – dispense the liquid into new tube
Side blue button = ejects the tip
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What technique is used to separatethe cut up pieces (fragments) of DNA?
electrophoresis
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What is the name of the gel thatis used to support the fragments during
this separation?
agarose
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Convert 0.6 mL to L
600 L
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What is the size (in bp) of this fragment?
6,527 bp
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Given the following fragments:500 bp 5,000 bp
2500 bp 25,000 bp Which fragment in the gel below is
the 25,000 bp fragment?
A
A B C D
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What is the charge on DNA?
What gives it this charge?
Negative
The phosphate group
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What are the places where the enzyme cuts DNA called?
Restriction sites
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A piece of DNA was cut by 3 different enzymes. The standard is in well A. How many “cuts” were made by the enzyme in well
B?
Two cuts
A B C D