b.sc. (biotechnology) degree programme

St. Peter’s Institute of Higher Education and Research

(Declared under section 3 of UGC Act 1956) Avadi, Chennai – 600 054.

B.Sc. (BIOTECHNOLOGY)

DEGREE PROGRAMME

(I to VI SEMESTERS)

REGULATIONS – 2016

REGULATIONS AND SYLLABI UNDER CHOICE

BASED CREDIT SYSTEM

(Effective from the Academic Year 2016-2017)

B.Sc. (BIOTECHNOLOGY) DEGREE PROGRAMME

Regulations – 2016

CHOICE BASED CREDIT SYSTEM (CBCS)

(Effective from the Academic Year 2016-‘2019) 1. Eligibility:

Candidates who passed Higher secondary Examinations with Physics, Chemistry, Biology or

Mathematics conducted by the Government of Tamil Nadu or an Examinations accepted

by the University as equivalent thereto are eligible for admission to three year B.Sc.

Programme in Biotechnology.

2. Duration:

Three years comprising 6 Semesters. Each semester has a minimum of 90 working days

with a minimum of 5 hours a day.

3. Medium:

English is the medium of instruction and examinations except for the language subjects.

4. Eligibility for the Award of Degree:

A candidate shall be eligible for the award of degree only if he/she has undergone the

prescribed course of study in the University for a period of not less than three academic

years (6 semesters), passed the examinations of all the six semesters prescribed

carrying 139 credits and also fulfilled such conditions as have been prescribed thereof.

5. Choice Based Credit System:

Choice Based Credit System is followed with one credit equivalent to one hour for theory

paper and two hours for a practical work per week in a cycle of 18 weeks (that is, one

credit is equal to 18 hours for each theory paper and one credit is equal to 36 hours for a

practical work in a semester in the Time Table. The total credit for the

B.Sc.(Biotechnology) Degree Programme (6 semesters) is 139 credits.

6. Weightage for a Continuous and End Assessment:

The weightage for Continuous Assessment (CA) and End Assessment (EA) is 25:75

unless the ratio is specifically mentioned in the Scheme of Examinations. The question

paper is set for a minimum of 100 marks.

7. Course of Study and Scheme of Examinations:

I Semester

Code No.

Course Title

Credit

Marks

CA EA Total

116UTMT01 /

UTET01 / UHIT01

Part I : Language -I (Tamil / Telugu / Hindi) 3 25 75 100

116UEHT02 Part II: English –I 3 25 75 100

116UBTT03 Part - III

Core Sub:

Cell biology 4 25 75 100

116UBTP01 Practical 1* 4 40 60 100

116UBTT04 Allied –I: Microbiology 5 25 75 100

116UBTT05 Non Major Elective I: 2 25 75 100

116UCCP01 Soft Skills (Common to all UG Branches) 2 50 50 100

Total 23 215 485 700

* practical examination will be conducted at the end of second semester. Non Major Electives I:

1. Microbiology 2. Plant Physiology 3. Animal Physiology

4. Bio-Diversity

II Semester

Code No.

Course Title

Credit

Marks

CA EA Total

216UTMT01 /

UTET01 / UHIT01

Part – I: Language -II (Tamil / Telugu / Hindi) 3 25 75 100

216UEHT02 Part II: English –II 3 25 75 100

216UBTT03 Part - III Core Sub:

Molecular Developmental Biology 4 25 75 100

216UBTP01 Practical 2* 4 40 60 100

216UBTT04 Allied –II: Chemistry 5 25 75 100

216UBTT05 Non Major Elective II : 2 25 75 100


Total 23 215 485 700

* practical examination will be conducted at the end of second semester.

Non Major Elective II: 1. Chemistry

2. Evolution 3. Behavioural Biology 4. Basics of Ecology

III Semester

Code No.

Course Title

Credit

Marks

CA EA Total

316UTMT01 / UTET01 / UHIT01

Part – I Language -III (Tamil / Telugu / Hindi) 3 25 75 100

316UEHT02 Part – II English –III 3 25 75 100

316UBTT03 Part - III Core Sub:

Genetics 5 25 75 100

316UBTP01 Genetics and Biochemistry

(Practical)

6 40 60 100

316UBTT04 Allied : Paper –I – Biochemistry 4 25 75 100

316UBTT05 Common Elective-I 2 50 50 100


Total 2 240 460 700

Common Elective-I Public Health Management

IV Semester

Code No.

Course Title

Credit

Marks

CA EA Total

416UTMT01 / UTET01 / UHIT01

Part – I Language-IV (Tamil / Telugu / Hindi) 3 25 75 100

416UEHT02 Part – II English –IV 3 25 75 100

416UBTT03 Part - III

Core Sub:

Plant Biotechnology 4 25 75 100

416UBTP01 Plant Biotechnology (Practical ) 4 40 60 100

416UBTT04 Allied Paper-II - Biophysics and Biostatistics 5 25 75 100

416UEST01 Part IV : Environmental Science 2 25 75 100


Total 23 215 485 700

V Semester

Code No.

Course Title

Credit

Marks

CA EA Total

516UBTT01

Core Sub:

Animal and Medical Biotechnology 4 25 75 100

516UBTT02 Bioinformatics 4 25 75 100

516UBTT03 Immunology 4 25 75 100

516UBTP01 Major practical: Animal, Medical

Biotechnology and Immunology.

4 40 60 100

516UBTT04 Elective I : Pharmaceutical Biotechnology 5 25 75 100

516UBTT05 Common Elective II: 2 50 50 100

516UVET01 Part IV : Value Education (Common to all UG

Branches)

2 25 75 100

Total 25 215 485 700

Elective I : Pharmaceutical Biotechnology Common Elective II: Integrated Agriculture Biology

VI Semester

Code No.

Course Title

Credit

Marks

CA EA Total

616UBTT01 Core Sub:

Genetic Engineering 4 25 75 100

616UBTT02 Bioprocess Technology 4 25 75 100

616UBTP01 Major practical: Genetic Engineering

and Bioprocess ( Practical)

5 40 60 100

616UBTT03 Elective II : Microbial Biotechnology 5 25 75 100

616UBTT04 Elective III : Environmental Biotechnology 5 25 75 100

616UEAT01 Part IV : Extension Activity (Common to all UG

Branches)

1 - - -

Total 24 140 360 500

8. Passing Requirements: The minimum pass mark (raw score) be 40% in End

Assessment (EA) and 40% in Continuous Assessment (CA) and End Assessment (EA) put together. No minimum mark (raw score) in Continuous Assessment (CA) is prescribed

unless it is specifically mentioned in the Scheme of Examinations.

9. CLASSIFICATION OF SUCCESSFUL CANDIDATES:

PART – I TAMIL/OTHER LANGUAGES: TAMIL/OTHER LANGUAGES OTIIER THAN ENGLISH: Successful candidates passing the examinations for the Language and securing the marks (i) 60 percent and above and (ii)

50 percent and above but below 60 percent in the aggregate shall be declared to have

passed the examination in the FIRST and SECOND Class respectively. All other successful

candidates shall be declared to have passed the examination in the THIRD Class.

PART – II ENGLISH:

ENGLISH: Successful candidates passing the examinations for English and securing the marks (i) 60 percent and above and (ii) 50 percent and above but below 60 percent in

the aggregate shall be declared to have passed the examination in the FIRST arid

SECOND Class respectively. All other successful candidates shall be declared to have

passed the examination in the THIRD Class.

PART - III CORE SUBJECTS, ALLIED SUBJECTS, AND PROJECT/ELECTIVES :

Successful candidates passing the examinations for Part-III Courses together and securing the marks (i),60 percent and above (ii) 50 percent and above but below 60 percent in the aggregate of the marks prescribed for the Part-III Courses together shall

be declared to have passed the examination in the FIRST and SECOND Class

respectively. All other successful candidates shall be declared to have passed the

examinations in the THIRD Class. .

PART-IV: Passing requirement as given in para 8 is applicable for Environmental Studies (EVS) and Value Education but there is no classification of successful candidates.

Extension Activity is rated as satisfactory by the Head of the Department as requirement for the award of degree.

10. Grading System: Grading System on a 10 Point Scale is followed with 1 mark = 0.1

Grade point to successful candidates as given below.

9

CONVERSION TABLE (1 mark = 0.1 Grade Point on a 10 Point Scale)

Range of Marks Grade Point Letter Grade Classification

90 to 100 9.0 to 10.0 O First Class

80 to 89 8.0 to 8.9 A First Class

70 to 79 7.0 to 7.9 B First Class

60 to 69 6.0 to 6.9 C First Class

50 to 59 5.0 to 5.9 D Second Class

40 to 49 4.0 to 4.9 E Third Class

0 to 39 0 to 3.9 F Reappearance

Procedure for Calculation

Cumulative Grade Point Average (CGPA) = Sum of Weighted Grade Points

Total Credits

= ∑ (CA+EA) C

∑C

Where Weighted Grade Points in each Course = Grade Points (CA+EA)

multiplied by Credits

= (CA+EA)C

Weighted Cumulative Percentage of Marks(WCPM) = CGPAx10

C- Credit, CA-Continuous Assessment, EA- End Assessment

11. Effective Period of Operation for the Arrear Candidates: Two Year grace period is provided for

the candidates to complete the arrear examination, if any.

Registrar

1

VISION & MISSION OF THE DEPARTMENT:

Vision

To produce higher caliber Biotechnologists to attain new heights in bioinformatics and bioprocess technology as per

industrial needs and to provide leaders in the field of Biotechnology.

Mission

To progress the department to attain center of excellence in bioinformatics and bioprocess technologies by providing

best Undergraduate, Postgraduate, Doctoral programs and R&D activities within a decade.

OBJECTIVES OF THE DEPARTMENT

1. To impart basic knowledge and skills of various aspects of biotechnology

2. To train the students for industrial need and to pursue further education

3. To inculcate entrepreneurship among the students so as to start their own ventures in the field of

biotechnology.

DESCRIPTION OF THE PROGRAMME

The B.Sc. Biotechnology program is a three-year degree. In the first two years, students will tackle core subjects

to ensure that they receive a solid grounding in fundamentals. Students will then specialize in the final year,

making their choice from a wide range of options and research projects. Our biotechnology courses contain topics

covering all aspects of the applied biotechnology industry, such as intellectual property and patents,

commercializing technology etc.

1

PROGRAMME EDUCATIONAL OBJECTIVES:-

S. No. PEOs PEO’s

I

PEO 1

Ability to apply knowledge of biology, science and technology

II

PEO 2

Ability to communicate effectively with biotechnologists, other professionals and community at large. The ability to explain the impact of engineering solutions in

societal, cultural, global and environmental context

III

PEO 3 Contribute towards technological development through academic research and

industrial practices.

IV

PEO 4

To produce research oriented Biotechnology graduates who will be employable in academic/Industry sponsored research and also who will be pursuing higher studies.

V

PEO 5

Ability to get employment in biotechnology and related industry and also to set up an industry.

1

PROGRAMME OUTCOMES:

Graduates of the course will have strong background in the interface of modern biology and skill based courses and be able to use these tools in business/industry and/or institutes wherever necessary.

Graduates will identify, formulate, research literature, and analyze complex science problems reaching

substantiated conclusions using first principles of mathematics, natural science, and applied sciences.

Graduates will demonstrate knowledge and understanding of the science and management principles and apply these to one's own work, as a member and leader in a team, to manage projects and in

multidisciplinary environments.

Graduates of the course will have the preparation and ability to engage in independent and life-long learning in the broadest context of technological change.

Graduates of the course will function effectively as an individual, and as a member or leader in diverse

teams, and in multidisciplinary settings.

Graduates of the course will communicate effectively on complex science activities with the science community and with the society at large.

Graduates of the course will apply ethical principles and commit to professional ethics and responsibilities and norms of the engineering and technology practice.

Graduates of the course will design solutions for complex science problems and design system components or processes that meet the specified needs with appropriate consideration for public health

and safety, and cultural, societal, and environmental considerations.

Program Specific Outcome (PSO)

To enable the student to emerge as:

PSO – 1: An expert in Biotechnology subjects knowledge

PSO – 2: An expert in Biotechnology practical skills.

PSO – 3: Efficient researcher using biotechnology practical skills.

PSO – 4: Development of own entrepreneur skills in biotechnology industry

PSO – 5: Well versed in the field of various biotechnology fields (medical, microbial, agricultural, environmental, plant and animal).

16

Subject code 116UBTT03

Subject

Name

CELL BIOLOGY

L T P C

Name of the

faculty

3

1

0

4

OBJECTIVES:

This course aims at providing the necessary basic concepts to the students to demonstrate the

knowledge and understanding of in-depth concept of cellular structure and their functions.

COURSEOUTCOME:

CO No

Course Outcomes

Knowledge Level

CO1

To develop the basic knowledge about the organization of the unicellular

and multicellular organisms

K1, K2

CO2

Apply the basic concepts of differentiation of cells

K2

CO3

To develop a basic knowledge about subcellular events of the cells.

K1,K2

CO4

Understand the knowledge of molecular mechanisms of cells.

K1,K2

CO5

Understand the knowledge of cellular differentiation.

K1,K2

K1 – Remember; K2 – Understand; K3 – Apply; K4 – Analyze; K5 – Evaluate; K6 – Create

COURSE DELIVERY METHODS Class room lecture - Black board; Digital platforms (PowerPoint Presentation; SWAYAM,

NPTEL)

ASSESSMENT METHODS

DIRECT ASSESSMENT

Internal Assessment(IA)

University Semester Examination

Assignments

Seminars

16

116UBTT03 - CELL BIOLOGY

UNIT- I Organisation of living organisms – Unicellular to higher organisms – organs – tissues – cells.

UNIT II

Cell: The dynamic cell- the molecules of life- the architecture and types of cells- differentiation of cells into tissues.

UNIT- III

Biomembranes, transport across cell membranes- subcellular organization of eukaryotic cells- microscopy and cell architecture.

UNIT- IV

Genetic code and the synthesis of macromolecules: structure of nucleic acids- nucleic acid synthesis- DNA

replication- repair- recombination- protein secretion and sorting, folding, modification, and degradation of proteins.

UNIT – V

Molecular structure of genes and chromosomes: Regulation of transcription in bacteria and eukaryotic cell.

RNA processing and post-transcriptional control- cell. RNA processing and post-transcriptional control- Regulation of gene expression. Hormones, viruses and gene expression; Nuclear-Cytoplasmic interaction.

UNIT – VI

Molecular aspects of cell division and cell cycle- cellular energetics- cell motility- cell-to-cell signaling-

hormones and receptors. Recommended Texts:

1. Cooper, G.M. 2000. The Cell- A molecular approach, II Edn., A.S.M. Press, Washington DC.

2. Lodish, H., Berk, A., Zipursky, S.L., Matusudaria, P., Baltimore, D. and Darnell, J., 2000. Molecular Cell Biology, Media Connected, W.H. Freeman and Company, New York.

Reference Books:

1. Brown, T.A 2001. Gene Cloning & DNA analysis. Blackwell Science, London. 2. Benjamin Lewis, 2000. Genes VII. Oxford University Press, London.

Web Sites:

1. http://www.spc.cc.tx.us/biology/bio_links1.htm

http://www.spc.cc.tx.us/biology/bio_links1.htm

17

116UBTP01 - Practical 1

CO No

Course Outcomes

Knowledge Level

CO1

Students will acquire knowledge and perform

experiments in the basic cell biological techniques

K3, K4

CO2

Students will gain knowledge and hands-On-training

on basic microbial techniques.

K3, K4

COURSE DELIVERY METHODS Class room lecture - Black board; Digital platforms (PowerPoint Presentation; SWAYAM,

NPTEL)

ASSESSMENT METHODS

DIRECT ASSESSMENT

Internal Assessment(IA)

University Semester Examination

Assignments

Seminars

18

116UBTP01 - Practical 1*

A. Cell Biology: Microscopy- RBC and WBC counting- Enumeration of WBC- Differential leukocyte Count- Salivary gland preparation from Chironomous larva- Mitosis preparation

from onion root tip and meiosis preparation from grasshopper testis- Enumeration of

prokaryotic cell- Buccal smear preparation- Cell fractionation (nucleus, mitochondria-

Demonstration). B. Microbiology: Sterilization techniques: Preparation of media, inoculation techniques –

Pour plate, spread plate and dilution techniques. Demonstration of microbial contamination

on culture plates. Micrometry- Wet preparation: Hay infusion broth, Hanging drop- Simple staining, Differential staining- Capsule staining- Spore staining- Inoculation techniques:

Pour plate- spread plate- Dilution techniques. Biochemical tests for identification of bacteria:

- Catalase test- Oxidase test- Urease test- IMVIC test- LAO test- Gelatin liquefaction- Starch

Degradation- Carbohydrate fermentation- Viable Bacteria; - morphological identification of Fungi.

17

116UBTT04 – MICROBIOLOGY (Allied)

CO No

Course Outcomes

Knowledge Level

CO1

To develop basic knowledge about the structure and

functions of the cellular components.

K1

CO2

Apply the basic concepts of methods of microbial

classification and identification

K1, K2

CO3 To develop a basic knowledge about the role of

microbes in food production.

K1,K2

CO4

Understand the knowledge of microbes in multiple areas

including pharmaceuticals and fertilizer industries.

K1,K2

CO5

Understand the knowledge and applications of industrial use of microbes.

K2, K3

Allied-116UBTT04 –MICROBIOLOGY

UNITI History and Scope of Microbiology- Anatomy of Prokaryotes and EuUkaryotes-

Bacteria, Fungi, Algae, Protozoa and Viruses- structunre and functions of the cellular components- Growth and nutritioin- media and culture.

UNITII Classifi–cation of microbes- DNA analysis, Ribosomal RNA analysis- NumeriIcal

taxonomy-Molecular taxonomy- methods of microbial identification. UNITIII-Environmental Microbiology- role of microorganisms in the productivity of ecosystems- Role of microorganisms in food production; dairy and non-dairy products- fermented foods and alcoholic beverages- production of food (single cell protein), Fuel (ethanol). UNITIV-Medical Microbiology- Pathogenic microbes- Bacterial, Viral, Fungal and Protozoan diseases. Cure, control and prevention- Pharmaceuticals (antibiotics, vaccines etc.), Biofertilizers (BGA), Biopesticides, biopolymers, biosurfactants.

UNITV-Industrial use of microbes- fermentation products- bioconversions- products of industrial microbiology- Streptomyces, yeasts (Saccharomyces, Hansenula), Spirulina and Penicillium. Recommended texts: Pelczar, M.J., Chan, E.C.S., King, N.R., 2001. Microbiology- Concepts and Applications. Tata McGraw – Hill, New Delhi. Ananthanarayan, R. and Paniker, C.K.J. 2000. A text book of Microbiology. 6th edition. Orient Longman Ltd., Hyderabad. Pelzar. 2000. Microbiology. 5th edition. Tata McGraw Hill., New Delhi. Ingraham, J.L., and Ingraham, C.A. 2000. Introduction to microbiology, 2nd edition. Brooks/Cole, Thomson Learning, USA. Reference books: Kathleen Park Talaro and Talaro, A. 1999. Foundation in Microbiology, 3rd edition,

17

McGraw-Hill, New York. Cappuccino, J.G and Sharman, N. 1999. Microbiology: A Laboratory manual, 4th edition. Addition Wesley Longman Inc., New York. Daniel Lim. 1998. Microbiology, 2nd edition. McGraw-Hill, New York. Web site: http://science.nhmccd.edu/biol/microbio.html.

http://science.nhmccd.edu/biol/microbio.html

17

116UBTT04 – MICROBIOLOGY (Allied)

CO No

Course Outcomes

Knowledge Level

CO1

develop basic knowledge about the structure and


K1

CO2



K1, K2

CO3 develop a basic knowledge about the role of


K1,K2

CO4



K1,K2

CO5

Understand the knowledge and applications of industrial

use of microbes.

K2, K3

116UBTT05 - NON MAJOR ELECTIVE - I

Microbiology

UNIT- I History and Scope of Microbiology- Anatomy of Prokaryotes and Eukaryotes- Bacteria, Fungi, Algae, Protozoa and Viruses- structure and functions of the cellular components- Growth and nutrition- media and culture.

UNIT- II

Classification of microbes- DNA analysis, Ribosomal RNA analysis- Numerical taxonomy- Molecular taxonomy- methods of microbial identification.

UNIT- III Environmental Microbiology- role of microorganisms in the productivity of ecosystems- Role

of microorganisms in food production; dairy and non-dairy products- fermented foods and alcoholic beverages- production of food (single cell protein), Fuel (ethanol). UNIT- IV

Medical Microbiology- Pathogenic microbes- Bacterial, Viral, Fungal and Protozoan diseases.

Cure, control and prevention- Pharmaceuticals (antibiotics, vaccines etc.), Biofertilizers (BGA), Biopesticides, biopolymers, biosurfactants.

UNIT- V Industrial use of microbes- fermentation products- bioconversions- products of industrial

microbiology- Streptomyces, yeasts (Saccharomyces, Hansenula), Spirulina and Penicillium. Recommended texts:

1. Pelczar, M.J., Chan, E.C.S., King, N.R., 2001. Microbiology- Concepts and

Applications. Tata McGraw – Hill, New Delhi. 2. Ananthanarayan, R. and Paniker, C.K.J. 2000. A text book of Microbiology. 6th

edition. Orient Longman Ltd., Hyderabad.

3. Pelzar. 2000. Microbiology. 5th edition. Tata McGraw Hill., New Delhi.

4. Ingraham, J.L., and Ingraham, C.A. 2000. Introduction to microbiology, 2nd

edition. Brooks/Cole, Thomson Learning, USA.

Reference books:

1. Kathleen Park Talaro and Talaro, A. 1999. Foundation in Microbiology, 3rd edition,

McGraw-Hill, New York. 2. Cappuccino, J.G and Sharman, N. 1999. Microbiology: A Laboratory manual, 4th

edition. Addition Wesley Longman Inc., New York. 3. Daniel Lim. 1998. Microbiology, 2nd edition. McGraw-Hill, New York.

Web site:

1. http://science.nhmccd.edu/biol/microbio.html

http://science.nhmccd.edu/biol/microbio.html

116UBTT05 - NON MAJOR ELECTIVE – I Microbiology

CO No

Course Outcomes

Knowledge Level

CO1

develop basic knowledge about the structure and


K1

CO2



K1, K2

CO3 develop a basic knowledge about the role of


K1,K2

CO4



K1,K2

CO5

Understand the knowledge of industrial use of microbes.

K1,K2

116UBTT05 - NON MAJOR ELECTIVE – I ANIMAL PHYSIOLOGY

CO No

Course Outcomes

Knowledge Level

CO1

develop basic knowledge of blood and circulation of

animal physiology.

K1

CO2

gain knowledge and understand the concept about

cardiovascular system of animals.

K1, K2

CO3 gain basic knowledge about the role and functions

of respiratory system.

K1,K2

CO4

understand the knowledge about the role, function of

nervous system.

K1,K2

CO5

gain knowledge about the physiology, metabolisms of excretory system of animals.

K1,K2

116UBTT05 - NON MAJOR ELECTIVE – I BIO-DIVERSITY

CO No

Course Outcomes

Knowledge Level

CO1

develop basic knowledge Principles and methods of

taxonomy.

K1

CO2

gain knowledge and understand the Levels of

structural organization.

K1, K2

CO3 gain basic knowledge about the Outline

classification of plants, animals and

microorganisms.

K1,K2

CO4

understand the knowledge about the Natural history of

Indian subcontinent.

K1,K2

CO5

gain knowledge about the organisms of health and agricultural importance.

K2, K3

PLANT PHYSIOLOGY

Unit I. Photosynthesis: Light harvesting complexes; mechanisms of electron transport; photoprotective mechanisms; CO2 fixation-C3, C4 and CAM pathways.

Unit II. Respiration and photorespiration: Citric acid cycle; plant mitochondrial electron transport and ATP synthesis; alternate oxidase; photorespiratory pathway.

Unit III. Nitrogen metabolism: Nitrate and ammonium assimilation; amino acid

biosynthesis. Unit IV. Plant hormones: Biosynthesis, storage, breakdown and transport; physiological effects and mechanisms of action.

Unit V. Stress physiology: Responses of plants to biotic (pathogen and insects) and

abiotic (water, temperature and salt) stresses; mechanisms of resistance to biotic

stress and tolerance to abiotic stress.

20

ANIMAL PHYSIOLOGY

Unit I. Blood and circulation: Blood corpuscles, haemopoiesis and formed elements,

plasma function, blood volume, blood volume regulation, blood groups, haemoglobin,

immunity, haemostasis. Unit II. Cardiovascular System: Comparative anatomy of heart structure, myogenic

heart, specialized tissue, ECG – its principle and significance, cardiac cycle, heart as a pump, blood pressure, neural and chemical regulation of all above.

Unit III. Respiratory system: Comparison of respiration in different species, anatomical

considerations, transport of gases, exchange of gases, waste elimination, neural and chemical regulation of respiration.

Unit IV. Nervous system: Neurons, action potential, gross neuroanatomy of the brain and

spinal cord, central and peripheral nervous system, neural control of muscle tone and posture.

Unit V. Excretory system: Comparative physiology of excretion, kidney, urine

formation, urine concentration, waste elimination, micturition, regulation of

water balance, blood volume, blood pressure, electrolyte balance, acid-base balance.

21

BIO-DIVERSITY Unit I. Principles and methods of taxonomy:Concepts of species and hierarchical taxa,

biological nomenclature, classical and quantititative methods of taxonomy of plants, animals

and microorganisms. Unit II. Levels of structural organization: Unicellular, colonial and multicellular forms; levels of organization of tissues, organs and systems; comparative anatomy.

Unit III. Outline classification of plants, animals and microorganisms:Important

criteria used for classification in each taxon; classification of plants, animals and

microorganisms; evolutionary relationships among taxa. Unit IV. Natural history of Indian subcontinent: Major habitat types of the

subcontinent, geographic origins and migrations of species; common Indian mammals, birds; seasonality and phenology of the subcontinent.

Unit V. Organisms of health and agricultural importance: Common parasites and

pathogens of humans, domestic animals and crops.

22

216UBTT03 - MOLECULAR DEVELOPMENTAL BIOLOGY

CO No

Course Outcomes

Knowledge Level

CO1

develop basic knowledge about the cellular

regulations and signaling pathways.

K1

CO2

gain knowledge about the eukaryotic transcription

process in different model organisms.

K2

CO3 gain basic knowledge about the myogenesis

process in mammals.

K1,K2

CO4

understand the knowledge about the neurogenesis in

different animal models.

K1,K2

CO5

gain knowledge about the embryogenesis in drosophila.

K2,K3

27

SEMESTER – II

216UBTT03 –MOLECULAR DEVELOPMENTAL BIOLOGY

UNIT- I Cellular regulation – cell cycle control, cell signaling pathways and differentiation.

UNIT- II

Transcription, expression and regulation in eukaryotic development with slime mould and C.

elegans as model systems UNIT- III Myogenesis in mammals- growth factors: Mitogens and Oncogenes.

UNIT- IV Neurogenesis in Drosophila and Mice- Regional specification in Drosophila.

UNIT- V Embryogenesis- Mammalian homologs in Drosophila ANT-C and BC-X.

Recommended Texts:

1. Gilbert, S. 2000. Developmental Biology. Seventh edition. Sinaver Associates Inc.

Publishers, MA. USA. 2. Tait, R.C. 1997. An Introduction to Molecular Biology, Horizon Scientific Press,

England.

Reference Books:

1. Lodish, H., Berk, A., Zipursky, S.L., Matsudaria, P., Baltimore, D. and Darnell,

J. 2000. Molecular Cell Biology. Media Connected. W.H. Freeman and

Company, New York. 2. Freifelder, D. 1990. Essentials of molecular biology. Narosa Publishing House, New

Delhi.

3. Watson, J.D., and Hopkins, N.H., Roberts, J.W., Steitz, J.A. and Weiner, A.M. 1988,

Molecular biology of the gene. 4th edition. Benjamin and Cummings Publishing Company, Inc., California.

Web Site:

1. http://web.wi.mit.edu/sive/pub/generallinks.html

http://web.wi.mit.edu/sive/pub/generallinks.html

28

216UBTP01 - Practical – 2

CO No

Course Outcomes

Knowledge Level

CO1


experiments in the molecular development biology.

K3, K4

CO2

Students will gain knowledge and hands-On-training

on basic volumetric analysis, aromatic primary and

secondary metabolites.

K3, K4

29

216UBTP01 - Practical – 2 A – Molecular Developmental Biology: Observation of living gametes (Grasshopper / Frogs).

Induction of ovulation and early fertilization in Bull frog - observation of development stages- gastrulation and organogenesis.- Observation of living chick embryos- cleavage and

gastrulation- Wound healing- cell aggregation in frog embryos- hormones in amphibian

metamorphosis. B- Chemistry: Volumetric analysis: Acidimetry- Alkalimetry- Permanganametry-

Dichlorometry- Iodimetry- Complexemetry- Analysis of Organic compounds with one

functional group: aldehyde, ketone, carboxylic acid, aromatic primary and secondary amine, phenol, aromatic ester, alcohol, nitrocompound, carbohydrates.

30

Allied 216UBTT04 – CHEMISTRY

CO No

Course Outcomes

Knowledge Level

CO1

Develop basic knowledge about the structure of ams

and its basic theory.

K1

CO2

Gain knowledge about the basic property of materials

on earth.

K2

CO3 Gain basic knowledge about the chemical changes

around our environment.

K1,K2

CO4

Understand the knowledge about the organic chemistry

and energy.

K1,K2

CO5

Gain knowledge about the chemistry in the living world.

K2,K3

31

Allied

216UBTT04 - CHEMISTRY

UNIT- I Structure of Atoms

Dalton’s Atomic theory- Subatomic particles- concepts of atoms and molecules- symbols for elements- electronic configuration of atoms- isotopes- shapes of atomic orbitals-periodical

table- periodic classification- periodicity- valency- chemical bond. UNIT- II Materials on earth

Properties of gas, liquid, solid, compound, mixtures, solutions, colloids, suspension- Acids, bases and salts- Conductors and non-conductors.

UNIT- III

Changes around us Slow and fast changes- reversible and irreversible reactions- exothermic and endothermic reactions- condition of chemical reactions- types of chemical reactions- mole concept and stoichiometry in chemical reaction- order of chemical reaction- technique used to determine

chemical reactions. UNIT- IV Organic chemistry and energy

Organic compounds- classification- functional groups- aromatic, aliphatic, heterocyclic

compounds- alkanes in gasoline- fuel from biogas, coal, hydrogen . UNIT- V Chemistry in living world

Physical and chemical properties of amino acids and proteins- properties and kinetics of

enzymes- thermodynamics. Recommended texts:

1. Mathews, P. 1996. Advanced chemistry, Cambridge University Press, Low Prize

Edition., Oxford. 2. Lee, J.D. 2001. Inorganic Chemistry. Blackwell Science., London.

3. Negi, A.S., and Anand. 2001. A text book of physical chemistry. Taj Press., New Delhi.

4. Sony, P.L. 2000. A text book inorganic Chemistry. Sultan Chand & Sons., New Delhi.

Reference books:

1. Voet, D. and Voet, J.G. 1995. Biochemistry, 2nd edition. John Wiley and Sons, Inc., New York.

2. Lehninger, A.L., Nelson D.L., and Cox, M.M. 1993. Principles of Biochemistry. 2nd

edition. CBS Publishers & Distributors, Delhi. 3. Amend, J.R., Mundy, B.P. and Armld, M.T. 1990. General Organic & Biological

Chemistry. Saunders College Publishing., London.

4. Greenwood, N.N. and Earnshaw, A. 1989. Chemistry of the Elements. Maxwell Macmillan intl. Ed., London.

5. Cotton, F.A and Wilkinson, G. 1989. Inorganic Chemistry. John Wiley and Sons, Inc.,

NewYork. 6. Finar, I.L. 1986. Organic Chemistry. Volume 1 & 2, ELBS., London.

Websites:

1. http://dir.yahoo.com/Science/Chemistry/

2. http://www.chemistry.mcmaster.ca/faculty/bader/aim/

http://dir.yahoo.com/Science/Chemistry/

http://www.chemistry.mcmaster.ca/faculty/bader/aim/

32

216UBBT05 - NON MAJOR ELECTIVE II CHEMISTRY

CO No

Course Outcomes

Knowledge Level

CO1

develop basic knowledge about the structure of ams

and its basic theory.

K1

CO2

gain knowledge about the basic property of materials

on earth.

K2

CO3 gain basic knowledge about the chemical changes

around our environment.

K1,K2

CO4

understand the knowledge about the organic chemistry

and energy.

K1,K2

CO5

gain knowledge about the chemistry in the living world.

K2,K3

33

216UBBT05 - NON MAJOR ELECTIVE II

CHEMISTRY

UNIT I. Structure of Atoms

Dalton’s Atomic theory- Subatomic particles- concepts of atoms and molecules- symbols for elements- electronic configuration of atoms- isotopes- shapes of atomic orbitals-periodical table- periodic classification- periodicity- valency- chemical bond.

UNIT II. Materials on earth

Properties of gas, liquid, solid, compound, mixtures, solutions, colloids, suspension- Acids,

bases and salts- Conductors and non-conductors. UNIT III. Changes around us Slow and fast changes- reversible and irreversible reactions- exothermic and endothermic

reactions- condition of chemical reactions- types of chemical reactions- mole concept and

stoichiometry in chemical reaction- order of chemical reaction- technique used to determine chemical reactions.

UNIT IV. Organic chemistry and energy Organic compounds- classification- functional groups- aromatic, aliphatic, heterocyclic

compounds- alkanes in gasoline- fuel from biogas, coal, hydrogen . UNIT V. Chemistry in living world

Physical and chemical properties of amino acids and proteins- properties and kinetics of enzymes- thermodynamics.

Recommended texts:

1. Mathews, P. 1996. Advanced chemistry, Cambridge University Press, Low Prize

Edition., Oxford. 2. Lee, J.D. 2001. Inorganic Chemistry. Blackwell Science., London.

3. Negi, A.S., and Anand. 2001. A text book of physical chemistry. Taj Press., New Delhi.

4. Sony, P.L. 2000. A text book inorganic Chemistry. Sultan Chand & Sons., New Delhi.

Reference books:

1. Voet, D. and Voet, J.G. 1995. Biochemistry, 2nd edition. John Wiley and Sons, Inc.,

New York. 2. Lehninger, A.L., Nelson D.L., and Cox, M.M. 1993. Principles of Biochemistry. 2nd

edition. CBS Publishers & Distributors, Delhi.

3. Amend, J.R., Mundy, B.P. and Armld, M.T. 1990. General Organic & Biological

Chemistry. Saunders College Publishing., London. 4. Greenwood, N.N. and Earnshaw, A. 1989. Chemistry of the Elements. Maxwell

Macmillan intl. Ed., London. 5. Cotton, F.A and Wilkinson, G. 1989. Inorganic Chemistry. John Wiley and Sons, Inc.,

NewYork. 6. Finar, I.L. 1986. Organic Chemistry. Volume 1 & 2, ELBS., London.

Websites:





34

216UBBT05 - NON MAJOR ELECTIVE EVOLUTION

CO No

Course Outcomes

Knowledge Level

CO1

develop basic knowledge about the Emergence of

evolutionary thoughts.

K1

CO2

gain knowledge about the Origin of molecules.

K2

CO3 gain basic knowledge about the Origin of cells and

unicellular evolution.

K1,K2

CO4

understand the knowledge about the Evolutionary

history.

K1,K2

CO5

gain knowledge about the Human evolution in the living world.

K2,K3

EVOLUTION Unit I. Emergence of evolutionary thoughts: Lamarck; Darwin–concepts of variation,

adaptation, fitness and natural selection. Unit II. Origin of molecules: Origin of basic biological molecules; abiotic synthesis of organic monomers and polymers.

Unit III. Origin of cells and unicellular evolution: Evolution of prokaryotes; origin of eukaryotic cells; evolution of unicellular eukaryotes.

Unit IV. Evolutionary history: The evolutionary time scale; eras, periods and epoch;

major events in the evolutionary time scale. Unit V. Human evolution: Stages in primate evolution including human.

35

216UBBT05 - NON MAJOR ELECTIVE BEHAVIOURAL BIOLOGY

CO No

Course Outcomes

Knowledge Level

CO1

develop basic knowledge about the

K1

CO2

gain knowledge about the Origin of molecules.

K2

CO3 gain basic knowledge about the Origin of cells and

unicellular evolution.

K1,K2

CO4

understand the knowledge about the Evolutionary

history.

K1,K2

CO5

gain knowledge about the Human evolution in the

living world.

K2,K3

BEHAVIOURAL BIOLOGY

Unit I. Approaches and methods in study of Behavior.

Unit II. Proximate and ultimate causation; altruism and evolution-group selection, kin

selection, reciprocal altruism. Unit III. Neural basis of learning, memory, cognition.

Unit IV. Sleep and arousal; biological clocks.

Unit V. Development of behavior; social communication; social dominance. Use of space and territoriality.

37

216UBBT05 - NON MAJOR ELECTIVE BASICS OF ECOLOGY

CO No

Course Outcomes

Knowledge Level

CO1

Development of basic knowledge about the basics

concepts on ecology

K1

CO2

Gain knowledge about the population of the ecology.

K2

CO3 Gain basic knowledge about the community of the

ecology.

K1,K2

CO4

Understand the knowledge about the ecological

succession.

K1,K2

CO5

Gain knowledge about the different forms of eco-system.

K2,K3

BASICS OF ECOLOGY

Unit I. The Environment: Physical environment; biotic environment; biotic and abiotic

interactions. Symbiosis. Concept of habitat and niche; Unit II. Population ecology: Characteristics of a population; population growth curves;

population regulation; life history strategies (r and K selection); concept of metapopulation – demes and dispersal, interdemic extinctions, age structured

populations.

Unit III. Community ecology: Nature of communities; community structure and

attributes; levels of species diversity and its measurement; edges and ecotones.

Unit IV. Ecological succession: Types; mechanisms; changes involved in succession; concept of climax.

Unit V. Ecosystems: terrestrial (forest, grassland) and aquatic (fresh water, marine, eustarine).

53

III SEMESTER

316UBTT03 - GENETICS

CO No

Course Outcomes

Knowledge Level

CO1

Develop basic knowledge about the history and

classical genetics.

K1

CO2

Gain knowledge about the basic concepts of blood

grouping and genetics behind it.

K2

CO3 Gain basic knowledge about the genetic mapping.

K1,K2

CO4

Understand the knowledge about the Evolutionary

history of DNA as genetic material.

K1,K2

CO5

Gain knowledge about the Human genome project.

K2,K3

316UBTT03 - GENETICS

UNIT I

Classical Genetics – Mendelian laws, monohybrid and dihybrid inheritance. Chromosome

structure and organization in prokaryotes and eukaryotes.

UNIT II

Multiple alleles and blood group antigens. Sex chromosomes and sex linked inherited disorders- X linked recessive, dominant inheritance, gender defective phenotypes.

UNIT III

Linkage, Crossing over and genetic mapping of chromosomes.

UNIT IV Identification of the DNA as the genetic material. Classical experiments of Hershey Chase,

Avery McLeod etc. Genetic recombination in bacteria: Conjugation, transduction, and transformation.

UNIT V Mutagens and Mutation. Principles of variation and selection process of speciation genetic

drift, pedigree analysis and Human genome project.

BOOKS FOR STUDY 1. Lewis, R.2001. Human genetics- concepts and application. 4th edition. McGraw

Hill.

2. Griffiths, Miller, J.H., An introduction to genetic analysis W.H.Freeman. New York.

3. Winter, P.C., Hickey, G.J. and Fletcher, H.L.2000. Instant notes in

genetics.Viva books, Ltd. 4. Gardener E.J. Simmons M.J.Slustad DP. 1991. Principles of Genetics. 5. Goodenough U. 1985. Genetics. Hold Saunders international.

54

316UBTP01 - GENETICS AND BIOCHEMISTRY - (PRACTICAL)

CO No

Course Outcomes

Knowledge Level

CO1


experiments in the basic quantitative and qualitative

analysis of biochemistry

K3

CO2


experiments in the genetics.

K3

316UBTP01 - GENETICS AND BIOCHEMISTRY - (PRACTICAL)

Biochemistry Practical VOLUMETRIC ANALYSIS:

Estimation of Glycine by formal titration – Estimation of glucose by Benedict’s method – Estimation of ascorbic acid using dichlorophenol indophenol as link solution.

QUALITATIVE AND QUANTITAIVE ANALYSIS:

Qualitative analysis of carbohydrates – Glucose, Fructose, Lactose, maltose and sucrose -

Qualitative analysis of amino acids: Arginine, cystein, tryptophan and tyrosine. QUANTITATIVE ANALYSIS: Colorimetric estimation a) protein by Lowry’s method, b)DNA using diphenylamine, c)

Glucose using orthotoludine method and d)Phosphorous by Fiske – Subba Rao method: Separation of amino acids by Paper chromatography.

Genetics Practical Preparations of culture medium and culture of Drosophila – methods of maintenance –

identifications of species and mutants. Identifications of human blood groups – mitotic stages of onion (Alium ceepa) root tip and meiotic stages of cockroach testes – giant chromosomes from Chironomous larvae/ Drosophila salivary glands.

ALLIED 316UBTT04 – BIOCHEMISTRY

55

CO No

Course Outcomes

Knowledge Level

CO1

Develop basic knowledge about the structure and

chemical properties of major metabolites.

K1

CO2

Gain knowledge about the basic concepts of structure and properties of Vitamins and Hormones.

K2

CO3 Gain basic knowledge about the principles and metabolic pathways of major metabolites.

K1,K2

ALLIED 316UBTT04 -BIOCHEMISTRY

UNIT I Structure, chemistry and properties of Carbohydrates; Lipids; and Proteins:

UNIT II

Classification of porphyrins, their structure and properties; structure of metalloporphyrins- haeme and chlorophyll; Vitamins and Hormones.

UNIT III

Principles of Bio-energetic; Metabolism of carbohydrates, fats, proteins, purines, pyrimidines- their biosynthesis & degradation; mechanism of oxidative phosphorylation & its inhibitors, photo phosphorylation.

UNIT IV

Prostaglandins, leukotrienes, thromboxines, interferons and interleukins; antibodies; alkaloids; plant and animal pigments.

UNIT V

Separation methods: Chromatography - electrophoresis and immunoelectrophoresis, high voltage electrophoresis and isoelectric focusing. Isolation methods – centrifugation, ultra – centrifugation, density gradient centrifugation.

BOOKS FOR STUDY

1. Sathyanarayana. U. 2002. Biochemistry. Books and allied Pvt. Ltd.

2. Murray, R.K., Granner, D.K., Mayes, P.A. and Rodwell, V.W. 2000. 3. Harper’s Biochemistry, 4th edition. McGraw-Hill. 4. Stryer, L. 1999. Biochemistry, 4th edition. W.H.Freeman & Company, New York.

5. Zubey, G.L. 1998. Biochemistry, 4th edition. McGraw-Hill.

6. Voet, D.and Voet, J.G.1995. Biochemistry, 2nd edition. John Willey and Sons, Inc.

7. Lehninger, A.L., Nelson, D.L and Cox, M.M.1993. Principles of Biochemistry, 8. 2nd edition. CBS Publishers and Distributors, Delhi.

56

Common Elective-I (316UBTT05)- III Semester

PUBLIC HEALTH MANAGEMENT

COURSE OUTCOME

1. Create awareness on disease

2. Understand the principles of disease management.

3. Provide knowledge about stem cells.

UNIT - I

Health – Basic concepts and Definition; Need for good health; factors affecting health, Basic

sanitation and personal hygiene – Food (Balanced diet) food habits and cleanliness, food

adulterants, avoiding smoking, drugs and alcohol.

UNIT - II

Public Health: Communicable diseases, Mode of disease transmission (Epidemic and endemic

diseases), Vaccination, Management of Hygiene in public places (Railway stations, Bus stands and

other public places) hospitals – Nosocomial / Hospital acquired infections and hygiene in

Educational institutions. UNIT - III

Occupational Health and Safety: Occupational health and hazards – physical, chemical and

biological hazards. Occupational diseases – Prevention and control. Health protection measures for

workers – health education – first aid. Management of medical emergencies. UNIT - IV

Disease: Introduction - principles of disease control - Infection- portal of entry; Disease- types of

diseases (Deficiency, infection, pollution diseases). Microbial flora of human. Host - Parasite

relationship. Management of communicable and non-communicable diseases. UNIT - V

Health and Disease: Basic concepts and Definition, Disease control and Levels of prevention,

Determinants and indicators of health, Health situation and trends in India. International bodies in

health protection and promotion. Role of quarantine rules, laws and enforcement in the preventive

measures of pandemic diseases.

REFERENCE BOOKS

1. Ananantanarayan, R. and Paniker, C.J.K “Textbook of Microbiology” 8th edition. Universities

Press, Orient Blackswan, 2005.

2. Park K, “Textbook of Preventive & Social Medicine” 22nd edition, Banarsidas Bhanot

publishers, 2013.

3. Roger Detels, Robert Beaglehole, Mary Ann Lansang, Martin Gulliford., “Oxford Textbook of

Public Health”, 5th edition. Oxford press, 2011.

57

416UBTT03 - PLANT BIOTECHNOLOGY

CO No

Course Outcomes

Knowledge Level

CO1

Develop basic knowledge about the plant genome

and whole genome organization.

K1

CO2

Gain knowledge about the mechanisms of plant

derived vectors and its functions.

K2

CO3 Gain basic knowledge about the applications of

plant derived vaccines.

K1,K2


plant derived metabolites and hormones.

K1,K2

CO5 Gain basic knowledge about the principles and

different types of plant tissue culture.

K3

58

416UBTT03 - PLANT BIOTECHNOLOGY UNIT I

Plant genome: Organization, structure of representative plant genes and gene families in

plants – chloroplast genome organization of mitochondrial genome. UNIT II

Agrobacterium and crown gall tumors – Mechanism of T-DNA transfer to plants, Ti Plasmid vectors and its utility – Plant viral vectors. Symbiotic nitrogen fixation in Rhizobia.

UNIT III

Seed storage proteins. Regeneration of gene expression in plant transgenic plants and applications – plant vaccine and plant development.

UNIT IV

Plant Hormones – IAA, GA and cytokinins – molecular basis of action – phytochrome – role

in photomorphogeneisis – Regulation of gene expression – abscisic acid – and stress – induced promoter switches in the control of gene expression – Ethylene and fruit ripening.

UNIT V

Plant tissue culture – suspension cultured cells – haploid plants – Cloning of hosts – micropropagation – somatic emryogenesis – protoplast isolation and applications.

BOOKS FOR STUDY:

1. Kojima, Lee, H. and Kun, Y. 2001. Photosynthetic microorganisms in

Environmental Biotechnology. Springer – Verlag.

2. Sudhir, M. 2000. Applied Biotechnology and plant Genetics. Dominant

publishers and distributors. 3. Trivedi, P.C.2000. Applied Biotechnology: Recent Advances. PANIMA

Publishing corporation.

4. Reynolds, P.H.S. (ed). 1999. Inducible gene expression in plants. CABI

Publishing, U.K. pp 1-247. 5. Chrispeels, M. J. and sadava, D.F. 1994. Plants, genes and agriculture. Jhones

and Bartlett.

6. Ignacimuthu. 1996. Applied Plant Biotechnology. Tata McGraw – Hill. Lycett, G.W. and Grierson, D. (ed). 1990. Genetic Engineering of crop plants.

7. Grierson and Covey, S.N.1988. Plant Molecular biology. Blackie.

8. Trigiano, R.N. and Gray, D.J. 1996. Plant tissue culture concepts and laboratory 9. exercise. CRC Press. BocaRatin, New York.

10. Street, H.E. 1977. Plant tissue culture. Blackwell Scientific Publications oxford, London.

11. Narayanaswamy S. 1994. Plant cell and tissue culture. Tata McGraw Hill Publishing Company limited, New Delhi.

59

416UBTP01 - PLANT BIOTECHNOLOGY (PRACTICAL)

CO No

Course Outcomes

Knowledge Level

CO1


experiments in cell and tissue culture and

maintenance of culture.

K3

416UBTP01 - PLANT BIOTECHNOLOGY (PRACTICAL) Plant Biotechnology Hands on training in cell and tissue culture and maintenance of culture

lines - Callus development and micropropagation of plants – Protoplast manipulation – Agrobacterium technology – Electropropagation – Biolistic transformation – Southern and Northern hybridization.

60

416UBTT04 - BIOPHYSICS AND BIOSTATISTICS

CO No

Course Outcomes

Knowledge Level

CO1

Develop basic knowledge about the scope and

methods of biophysics and level of molecular

organization.

K1

CO2

Gain knowledge about the structure and chemical

nature of polysaccharides.

K2


biostatistics and different forms of plotting graphs.

K2, K3

65

ALLIED- 416UBTT04 - BIOPHYSICS AND BIOSTATISTICS

UNIT I

Scope and methods and biophysics – levels of molecular organization – detailed structure of protein molecules at primary, secondary, tertiary and quaternary levels.

UNIT II

Analysis of protein, protein interaction and protein – nucleic acid interaction. Structure and chemical nature of polysaccharides.

UNIT III

Statistics – collection, classification, tabulations of Statistical Data – Diagrammatic

representation – graphs – plotted curve – Sampling method and standard errors – random sampling – means – confidence limits – standard errors – variance.

UNIT IV

Measures of central tendency – measures of dispersion – Skew ness, kurtosis, moments – Correlations and regression.

UNIT V

Probablity distributions – Binomial and negative binomial, compound and multinomial distributions – Tests of significance – t tests – F tests – Analysis of variance – Spread sheets – Data entry – Graphics display – word processes.

BOOKS FOR STUDY

1. Creighton, T.E.2002. Protein structure and molecular propertie. W.H. Freeman and Company.

2. Micklos, D.A. and Freyes, G.A. 2002. DNA Science. Cold Spring harbour laboratory Press.

3. Sundar Rao P.S.S., Jesudian G. and Richard J. 1987. An introduction to Bio-

Statistics (2nd Ed). Prestographik, Vellore, India.

4. Gupta, S.P. 1997. BiostatisticalMethods, S.Chand and Sons. 5. Elhance, d.n.1972. Fundamentals of Statistics, Kitab Mhal, Allahabad.

6. Sokal, P.R and Rohif, F.R. 1969. Biometry: The Principles and Practice of 7. Statistics, in Biological Research, Freeman and company, San Francisco.

8. Snedecor, George, W.Cochran and William, G. 1967. Statistical Methods, Sixth edition, Oxford and IBH Publishing Co., Oxford

9. Federer W.R 1955. Experimental Design: Theory and Applications, Oxford Publishing Co. Pvt. Ltd., New Delhi.

66

416UEST01 - ENVIRONMENTAL STUDIES

Vision

The importance of environmental science and environmental studies cannot be disputed. The need for sustainable development is a key to the future of mankind. Continuing problems of pollution, loss of forget, solid waste disposal, degradation of environment issues like economic productivity and national security, Global warming, the depletion of ozone layer and loss of biodiversity have made everyone aware of environmental issues. The United Nations Conference on Environment and Development held in Rio de Janerio in 1992 and world Summit on Sustainable Development at Johannesburg in 2002 have drawn the attention of people around the globe to the deteriorating condition of our environment. It is clear that no citizen of the earth can afford to be ignorant of environment issues. Environmental management has captured the attention of health care managers. Managing environmental hazards has become very important.

Human beings have been interested in ecology since the beginning of civilization. Even our

ancient scriptures have emphasized about practices and values of environmental

conservation. It is now even more critical than ever before for mankind as a whole to have a clear understanding of environmental concerns and to follow sustainable development

practices. India is rich in biodiversity, which provides various resources for people. It is also

basis for biotechnology. Only about 1.7 million living organisms have been described and

named globally. Still many more remain to be identified and described. Attempts are made to

conserve them in ex-situ and in-situ situations. Intellectual property rights (IPRs) have become important in a biodiversity-rich country like India to protect microbes, plants and

animals that have useful genetic properties. Destruction of habitats, over-use of energy

resource and environmental pollution have been found to be responsible for the loss of a

large number of life-forms. It is feared that a large proportion of life on earth may get wiped out in the near future.

Inspite of the deteriorating status of the environment, study of environment has so far not received adequate attention in our academic programmes. Recognizing this, the Hon’ble

Supreme Court directed the UGC to introduce a basic course on environment at every level

in college education. Accordingly, the matter was considered by UGC and it was decided

that a six months compulsory core module course in environmental studies many be prepared and compulsorily implemented in all the University/Colleges of India. The experts

committee appointed by the UGC has looked into all the pertinent questions, issues and

other relevant matters. This was followed by framing of the core module syllabus for

environmental studies for undergraduate courses of all branches of Higher Education. We

are deeply conscious that there are bound to be gaps between the ideal and real. Geniune endeavour is required to minimize the gaps by intellectual and material inputs. The success

of this course will depend on the initiative and drive of the teachers and the receptive

students.

Unit 1 : Multidisciplinary nature of environmental studies Definition, scope and importance, need for public awareness. (2 lectures)

Unit 2 : Natural Resources :

Renewable and non-renewable resources : Natural resources and associated problems.

a. Forest resources : Use and over-exploitation, deforestation, case studies. Timber

extraction, mining, dams and their effects on forest and tribal people. b. Water resources : Use and over-utilization of surface and ground water, floods,

drought, conflicts over water, dams-benefits and problems.

c. Mineral resources : Use and exploitation, environmental effects of extracting and using mineral resources, case studies.

d. Food resources : World food problems, changes caused by agriculture and over-

grazing, effects of modern agriculture, fertilizer-pesticide problems, water

logging, salinity, case studies. e. Energy resources : Growing energy needs, renewable and non renewable energy

67

sources, use of alternate energy sources. Case studies.

f. Land resources : Land as a resource, land degradation, man induced landslides,

soil erosion and desertification. • Role of an individual in conservation of natural resources. • Equitable use of resources for sustainable lifestyles. (8 lectures)

Unit 3 : Ecosystems

• Concept of an ecosystem. • Structure and function of an ecosystem.

• Producers, consumers and decomposers. • Energy flow in the ecosystem. • Ecological succession.

• Food chains, food webs and ecological pyramids. • Introduction, types, characteristic features, structure and function of the following

ecosystems :- (a) Forest ecosystem

(b) Grassland ecosystem (c) Desert ecosystem

(d) Aquatic ecosystems (ponds, streams, lakes, rivers, oceans, estuaries) (6

lectures)

Unit 4 : Biodiversity and its conservation (8 lectures)

• Introduction – Definition : genetic, species and ecosystem diversity.

• Biogeographical classification of India • Value of biodiversity : consumptive use, productive use, social, ethical, aesthetic and

option values • Biodiversity at global, National and local levels.

• Inida as a mega-diversity nation • Hot-sports of biodiversity. • Threats to biodiversity : habitat loss, poaching of wildlife, man-wildlife conflicts. • Endangered and endemic species of India

• Conservation of biodiversity : In-situ and Ex-situ conservation of biodiversity.

Unit 5 : Environmental Pollution (8 lectures) Definition • Cause, effects and control measures of :-

a. Air pollution b. Water pollution

c. Soil pollution

d. Marine pollution e. Noise pollution f. Thermal pollution

g. Nuclear hazards • Solid waste Management : Causes, effects and control measures of urban and • industrial wastes. • Role of an individual in prevention of pollution.

• Pollution case studies. • Diaster management : floods, earthquake, cyclone and landslides.

Unit 6 : Social Issues and the Environment (7 lectures)

• From Unsustainable to Sustainable development

• Urban problems related to energy • Water conservation, rain water harvesting, watershed management • Resettlement and rahabilitation of people; its problems and concerns. Case

• Studies

• Environmental ethics : Issues and possible solutions. • Climate change, global warming, acid rain, ozone layer depletion, nuclear • accidents and holocaust. Case Studies. • Wasteland reclamation.

68

• Consumerism and waste products. • Environment Protection Act. • Air (Prevention and Control of Pollution) Act.

• Water (Prevention and control of Pollution) Act • Wildlife Protection Act

• Forest Conservation Act • Issues involved in enforcement of environmental legislation. • Public awareness.

Unit 7 : Human Population and the Environment (6 lectures)

• Population growth, variation among nations. • Population explosion – Family Welfare Programme. • VII

• Environment and human health. • Human Rights. • Value Education.

• HIV/AIDS. • Women and Child Welfare. • Role of Information Technology in Environment and human health. • Case Studies.

Unit 8 : Field Work

Visit to a local area to document environmental assets rivers/forest/grassland/hill/moutain.

Visit to a local polluted site – urban / Rural / Industrial / Agricultural Study of common plants, insects, birds.

Study of simple ecosystems-pond,river,hill slopes,ect. (Field work Equal to 5 lecture

hours)

69

516UBTT01 - ANIMAL AND MEDICAL BIOTECHNOLOGY

CO No

Course Outcomes

Knowledge Level

CO1

Develop basic knowledge about the basic principles

and important technologies adapted in the field of

biotechnology

K1

CO2

Gain knowledge about the history and major accepts

of medical biotechnology.

K2

CO3 Gain basic knowledge about the Health Disease

Diagnosis.

K1,K2

CO4 Gain basic knowledge about the different forms of

Vaccines and its applications.

K1,K2

CO5 Gain basic knowledge about the genetic

engineering of microbes and molecules.

K3

70

V Semester

516UBTT01 - ANIMAL AND MEDICAL BIOTECHNOLOGY

UNIT I

Basic principles of Biotechnology – manipulation of reproductive process – Artificial

insemination – freezing of semen – Embryo technology – in vitro maturation and fertilization – Pregnancy diagnosis – Assisted reproductive technology – cloning strategies – transgenic

animals. UNIT II

Historical aspects – Medical Biotechnology – Pathogenic microbes – Bacterial, Viral, Fungal and Protozoan disease – diagnosis using modern techniques – probes – Cure, control and prevention.

UNIT III

Health Disease Diagnosis: Hybridoma Technique, Monoclonal antibodies, application of Probes for diagnosis of existing and emerging disease in animal and human disease.

UNIT IV

Vaccines – Production of recombinant vaccines – bacterial, viral or parasitic infections – DNA Vaccines. Synthetic peptide, anti-idiotype, deletion mutant and vaccine vectored vaccine – Prophylaxis.

UNIT V Genetic engineering of Microorganisms and molecules – Recombinant DNA, DNA/RNA

probes – monoclones – diagnosis – Animal cell culture – maintenance and culture of primary, secondary and continuous cell lines – applications.

BOOKS FOR STUDY

1. Click, B.R. and Pasternark. 2002. Molecular Biotechnology: Principle and applications

of recombinant DNA. ASM press.

2. Ramasamy. P. 2002. Trends in Biotechnology, University of Madras of Publications, Pearl Press.

3. Kreuzer, H. and Massey, A. 2001. Recombinant DNA and Biotechnology: A guide for teachers, 2nd edition. ASM Press Washington.

4. Traven. 2001. Biotechnology. Tata McGraw – Hill. 5. Walker, J.M. and Gingold, E.B. 1999.Molecular biology and Biotechnology, 3rd edition.

Panima Publishing Corporation.

6. Jenkins, N. (ed). 1999 Animal cell Biotechnology: Methods and protocols. Humana press, New Jesey. Pp 1-302.

7. Ignacimuthu. 1996. Basic Biotechnology. Tata McGraw-Hill.

8. Puhler, A.V.C.H. 1993. Genetic engineering of Animals. VCH Publishers, Weinbeiim, FRG. Watson, J.D., Gilman, M., Witknowski, J. and Zoller, M. 1992. Recombinant

DNA (2nd ed) Scientific American Books, NY.

9. Murray, E.T. 1991. Gene transfer and expression protocols – Methods in Molecular biology Vol.7. Humana Press.

10. Watsen, J.D., Hopkins, N.H., Roberts, J.W. Steitz, J.A. and Weiner, A.M. 1987.Molecular biology of gene. Benjamin/ Cummings 4th Ed. Vol.1&2.

71

516UBTT02 – BIOINFORMATICS

CO No

Course Outcomes

Knowledge Level

CO1

Develop basic knowledge about the basic principles

and important Genomic and cDNA sequences.

K1

CO2

Gain knowledge about the Gene therapy and novel

drug discoveries.

K2

CO3 Gain basic knowledge about the importance of gene

expression and recent development in gene

expression studies.

K1,K2

CO4 Gain basic knowledge about the Sequence analysis.

K1,K2

CO5 Gain basic knowledge about Protein database and

DNA and protein functions.

K3

516UBTT02 - BIOINFORMATICS UNIT I

Genomic and cDNA sequences: output management from different biological output sources, gene prediction rules and software – Human Genome Project – Mutations, Population studies.

UNIT II Gene therapy: Analysis of genomic and proteomic information with respect to biological

systems – Genome application – Transgenic animals and plants – pathway regulatory networks. Drug design / discovery and identification, synthesis of new drugs; Management of diverse chemical libraries.

UNIT III

Gene expression: Microarrays and recent developments in expression analysis: Genes; Oncogenes – protooncogenes – Classification of Cancer types: Application of Microarrays in

Drug toxicity testing, metabolic pathways. UNIT IV Sequence analysis (Proteins and Nucleic acids) Sequence alignment

methods – Proteomics: Proteins analysis – structural comparisons – 2D gel, Mass spec, protein and antibody arrays.

UNIT V Protein Database: Comparison of Protein sequences and Database

searching – methods for protein structure prediction – conserved patterns in protein

sequences and structures – Comparison of protein 3D structures – predicting functions based on DNA and protein sequences.

BOOKS FOR STUDY:

1. Pennington, S.R. and Punn, M.J. 2002.Proteomics: from protein sequence to function. Viva books Pri. Ltd.

2. Maleolm and Goosfship. J. 2001. Genotype to phenotype, 2nd edition. Bios

Scientific Publishers Ltd.

72

3. Misener, S. and Krawetz. S.A. 2000. Bioinformatics: Methods and Protocols. Humana press.

4. Attwood, T.K. and Parry-Smith, D.J.1999. Introduction to Bioinformatics. Pearson Education Asia.

5. Primrose, S.B. 1998. Principle of genome analysis. 2nd edition. Blackwell Science.

6. Durbin, R., Eddy, S., Krogh, A. and Mitchison, G. 1998. Biological sequence analysis.

Cambridge University Press. 7. Friedman, C.P. and Wyatt. J.C. 1997. Computers and Machine: Evaluation methods in medicinal information. Springer-verlag, New York.

8. Bishop, M.J. and Rawhings. C.J. 1997. DNA and protein sequence analysis: A practical approach. Oxford University press. New press. 9. Kolodner, R.M. 1997. Computer in Health care: Computerizing large integrated health

networks. Springer – Verlag, New York. 516UBTT03 – IMMUNOLOGY

CO No

Course Outcomes

Knowledge Level

CO1

Develop basic knowledge about the basic concepts

about the antigens and other biological molecules.

K1

CO2

Gain knowledge about the purification of

mononuclear cells from peripheral blood cells.

K2

CO3 Gain basic knowledge about the Hybridoma and

monoclonal antibody production

K1,K2

CO4 Gain basic knowledge about the In situ and in vivo

characterization of cells from tissues.

K1,K2

CO5 Gain basic knowledge about the importance and

significance of Immunotechnology and infectious

diseases.

K2

73

516UBTT03 - IMMUNOLOGY UNIT I

Antigen: Isolation, purification and characterization of various antigens and haptens from pathogens and other biological molecules.

UNIT II

Purification of mononuclear cells from peripheral blood: Isolation and Characterization of T cell subsets; B cells and macrophages; Macrophage cultures; Assay for Macrophage activation; Isolation of dendritic cells.

UNIT III

Hybridoma and monoclonal antibody production: Production of antibodies: purification of antibodies, Quantification of Immunoglobulins, Immunodiagosis and Applications of

Monoclonal antibodies in biomedical research. UNIT IV

Assessment of delayed hypersensitivity reactions; In situ and in vivo characterization of cells from tissues; HLA typing.

UNIT V

Biology and assay of cytokines; Vaccine technology including DNA vaccines; Immunotechnology and infectious diseases.

BOOKS FOR STUDY

1. Ramasamy, P and R.E.B.Henna, 2002. Immunity and inflammation. University of Madras Publications Division, M/S. Pearl press, Chennai, India.

2. Parslow, T.G., Stites, D.P. and Terr, A.L. 2001. Medical immunology, 10th edition. McGraw-Hill publishing.

3. Goldsby, R.A., Kindt, T.J. and Osborne, B.A. 2000. Kuby immunology. 4th edition. Freeman and company.

4. Zola, H. 2000. Monoclonal antibodies. Bios Scientific Publishers ltd. 5. Roitt, I. 1996. Immunology. Black well Scientific Publications.

6. Weir, D. M. 1992. Immunological techniques. 3 Volumes. Black well Scientific Publishers.

74

516UBTP01 - ANIMAL, MEDICAL BIOTECHNOLOGY AND IMMUNOLOGY

(PRACTICAL)

CO No

Course Outcomes

Knowledge Level

CO1


experiments in Animal and Medical biological

experiments including preparation of cell culture.

K3

CO2

Students will get hands on experience in different

immunological assays for diagnosing diseases and its

mechanisms.

K3

516UBTP01 - ANIMAL, MEDICAL BIOTECHNOLOGY AND IMMUNOLOGY

(PRACTICAL)

Animal and Medical Biotechnology

Preparation of tissue culture medium and membrane filtration; preparation of single cell

suspension from spleen and thymus; Cell counting and cell viability; Macrophage monolayer

from PEC and measurement of phagocytic activity; Trypsinization of monolayer and sub culturing; Cryopreservation and thawing; Measurement of doubling time; Role of serum in

cell culture.

Immunology Blood groups and Rh Typing – Handling of animals and Raising Antibodies – Direct

agglutinations – Slide and Tube methods:- Whole cell agglutination – Slide and Tube methods – Whole cell agglutination – Slide and tube methods – Serotyping by slide

Agglutination – Indirect Agglutination test: Particle Agglutination – Letex Hbs Ag, ASLO – Passive Haemagglutination – TPH – Precipitation – Single Radial Immuno Diffusion (SRID) – Double Immuno Diffusion – Amboceptor titration (Demonstration) – ELISA (Demonstration) – Skin test for demonstration cutaneous hypersensitivity.

75

ELECTIVE I 516UBTT04 - PHARMACEUTICAL BIOTECHNOLOGY

CO No

Course Outcomes

Knowledge Level

CO1

Develop basic knowledge about the application of

microbes in Pharmaceutical biotechnology.

K1

CO2

Gain knowledge about the basic concept of

Pharmacokinetics and Pharmacodynamics.

K2

CO3 Gain basic knowledge about the Protein

engineering and its clinical aspects.

K1,K2

CO4 Gain basic knowledge about the Pharmacology and

Formulations.

K1,K2

CO5 Gain basic knowledge about the importance and

process of development of drug discovery and

development.

K2

ELECTIVE I

516UBTT04 - PHARMACEUTICAL BIOTECHNOLOGY

UNIT 1 Pharmaceutical biotechnology an introduction. Microbes in pharmaceutical industry. Formulation of biotech products including biopharmaceutical considerations (Microbiological Considerations). Shelf life of protein based pharmaceuticals. Delivery of proteins - Rate and target site - specific delivery. Site specific delivery of protein drugs.

UNIT 2

Pharmacokinetics and Phermacodynamics - Peptide and protein drugs. Elimination of protein

Therapeutics and Distribution of therapeutics, Protein binding of proteins therapeutics, Heterogeneity of protein therapeutics. Chemical modification of protein therapeutics and

immunogenicity. UNIT 3

Protein engineering, Peptide chemistry and Peptidomimetics, catalytic Antibodies,

Glycobiology and biosensors. Impact of biotechnology on drug discovery. Gene therapy - ex vivo and in vivo gene therapy. Hematopoietic growth Factors, Chemical description,

pharmacology, Pharmaceutical Concerns, clinical and Practice aspects. UNIT 4

Pharmacology and Formulations- Vaccines, Modern vaccine technologies, pharmaceutical

aspects. Monoclonal antibody -Based pharmaceuticals, development of antibody based therapeutics. Formulation of monoclonal antibody- Based therapeutically.

UNIT 5

Biotechnology products in pipeline - Drug development, Protein Pharmaceutical in development. Nucleic acid therapies in development. development of Adhesion molecules, glycoprotein, and carbohydrate based pharmaceuticals and other products of glycobiology.

76

BOOKS FOR STUDY

1. Daniel Figeys (Ed.) 2005. Industrial proteomics: Applications for Biotechnology and Pharmaceuticals. Wiley and Sons, Incorporated.

2. O. Kayser, R.H. Muller. 2004. Pharmaceutical Biotechnology - Drug Discovery and

clinical applications. Wiley - VCH. 3. Heonrich Klefenz. 2002. Industrial Pharmaceutical Biotechnology.

4. Leon Shargel, Andrew B. C. Yu, Susanna Wu-Pong and Yu Andrew B.C.2004. Applied

Biopharmaceutics and pharmacokinetics. McGraw- Hill Companies. 5. Sefania Spada, Garywalsh. 2004. Directory of approved biopharmaceutical. 6. Garywalsh. 2003. Biopharmaceutical, biochemistry and biotechnology.

7. Thomas Lengauer (Ed) 2002. Bioinformatics - from Genomes to drugs. Vol.I and II. Wiley - VCH.

8. JOHN F. Corpenter (ed.) Mark C. Manning. 2002. Rational design of stable formulation theory and practice (Pharmaceutical Biotechnology). Plenum, US. Ist edition.

9. D.I.A. Crommelin et al, 2002. Pharmaceutical biology. Amazon prome publications.

10. Werner kalow, UA Meyer and Rachel F Tyndale. 2001.

77

516UVET01 - VALUE EDUCATION (COMMON TO ALL UG BRANCHES)

PART- IV VALUE EDUCATION - III YEAR – FIFTH SEMESTER CREDITS : 2

Objective : Values are socially accepted norms to evaluate objects, persons, and situations

that form part and parcel of sociality. A value system is a set of consistent values and

measures. Knowledge of the values are inculcated through education. It contributes in forming true human being, who are able to face life and make it meaningful. There are different kinds of values like, ethical or moral values, doctrinal or ideological values, social

values and aesthetic values. Values can be defined as broad preferences concerning appropriate courses of action or outcomes. As such, values reflect a person’s sense of right

and wrong or what “ought” to be. There are representative values like, “Equal rights for all”,“Excellence deserves admiration”. “People should be treated with respect and dignity”. Values tend to influence attitudes and behavior and help to solve common human problems. Values are related to the norms of a culture.

Unit I: Value education-its purpose and significance in the present world – Value system –

The role of culture and civilization-Holistic living – Balancing the outer and inner – Body, Mind and Intellectual level- Duties and responsibilities.

Unit II : Salient values for life- Truth, commitment, honesty and integrity, forgiveness and

love, empathy and ability to sacrifice, care, unity , and inclusiveness, Self esteem and self

confidence, punctuality – Time, task and resource management – Problem solving and decision making skills- Interpersonal and Intra personal relationship – Team work – Positive

and creative thinking. Unit III : Human Rights – Universal Declaration of Human Rights – Human Rights

violations – National Integration – Peace and non-violence – Dr. A P J Kalam’s ten points for

englightened citizenship – Social Values and Welfare of the citizen – The role of media in

value building.

Unit IV: Environment and Ecological balance – interdependence of all beings – living and

non-living. The binding of man and nature – Environment conservation and enrichment.

Unit V : Social Evils – Corruption, Cyber crime, Terrorism – Alcoholism, Drug addiction – Dowry – Domestic violence – untouchability – female infanticide – atrocities against women-

How to tackle them. Books for Reference: 1. M.G.Chitakra: Education and Human Values,

A.P.H.Publishing Corporation, New Delhi, 2003

78

Common Elective- II (516UBTT05) V-Semester

Integrated Agriculture Biology

COURSE OUTCOME

Understand about the agriculture and the modern development in it. To understand the

challenges in the production and improvement of crops.

UNIT - I

History of Indian Agriculture, Green Revolution in India, Cropping patterns in India, Soil, Rainfall

Patterns in India. UNIT - II

Microbes in Agriculture and Food: microbial enzymes and their applications in food processing

and agro-chemical industries, agro-waste utilization, biodegradable polymers and their

applications. UNIT - III

Production and utilization of essential amino-acids, chemicals from micro-algae, Micorrhiza:

Applications in agriculture and forestry. UNIT - IV

Genetic Engineering for Crop Improvement: Plant cell and tissue culture, gene transfer techniques

into plant cells, application in agricultural and food industries. UNIT - V

Plant Pathology – Major crops and major diseases in India. Developing diseases, Drought, Salinity,

Cold tolerant crops in India and its economic importance. Post-harvest and storage of grains. REFERENCE BOOKS

1. Textbook of Agricultural Biotechnology by Dr. Ahindra Nag, PHI Learning Private Ltd.,

New Delhi, 2009.

2. Agricultural Biotechnology Edited by Arie Altman, Pub. Marcel Dekker, Inc., 1998

79

616UBTT01 - GENETIC ENGINEERING

CO No

Course Outcomes

Knowledge Level

CO1

Develop basic knowledge about the basic concepts of

genetic engineering and different cloning strategies.

K1

CO2

Gain knowledge about the recombinant technology

and DNA finger printing technologies.

K2

CO3 Gain basic knowledge about the DNA sequencing

and applications of Polymerase chain reaction in

genetic engineering techniques.

K1,K2

CO4 Gain basic knowledge about the Expression systems and their applications.

K1,K2

VI Semester

616UBTT01 - GENETIC ENGINEERING

UNIT I Restriction and modification systems in bacteria. Restriction

enzymes. Cloning vectors. Core techniques in gene manipulation:

Cloning strategies; Construction of gene libraries and Probe.

UNIT II Recombinant technology: gene cloning – Selection and screening

for recombinants – RFLP, DNA finger printing.

UNIT III

DNA sequencing, Polymerase chain reaction; Ligase chain reaction, site directed mutagenesis.

UNIT IV

Expression systems and their applications: Production of protein from cloned genes;

UNIT V Gene cloning and manipulation in research, medicine and

agriculture.

BOOKS FOR STUDY: Thiel. 2002. Biotechnology DNA to Protein: A laboratory Project.

Tata McGraw-Hill. Ring, C.J.A. and Blair, E.D.2001. Genetically Engineered viruses. Development and application. Bios Scientific publishers.

Davidson, E.H.2001. Genomic regulatory systems: Development

and evaluation. Academic press. Kreuzee and Massey, A. 2001. Recombinant DNA & Biotechnology.

80

ASM Press.

Mukhopadhyay, S.N.2001.Process Biotechnology fundamentals. Viva books.

Jognand, S.N. 2000.Gene Biotechnology. Hemalaya publishers. Walker, M. and Gingold, E.B. 1999. Molecular biology and Biotechnology, 3rd edition. Panama Publishing Corporation.

Old, R.W. and Primrose, S.B.1998. Principles of An introduction to Genetic Engineering Blackwell Science.U.K.

Brown, T.A. 1995. Gene cloning an introduction. Chapman & Hall. London.

616UBTT02 - BIOPROCESS TECHNOLOGY

81

CO No

Course Outcomes

Knowledge Level

CO1

Develop basic knowledge about traditional and

modern applications of biotechnological process.

K1

CO2

Gain knowledge about the different fermentation process and its application in the biotechnology

industry.

K2

CO3 Gain basic knowledge about the Enzymatic

bioconversion processes and different fermentation

techniques and its industrial application.

K1,K2

CO4 Gain basic knowledge and acquire knowledge on

metabolic stoichiometry and energetics.

K1,K2

CO5 Gain basic knowledge about the Kinetics of

microbial growth and product formation

K1,K2

82

616UBTT02 - BIOPROCESS TECHNOLOGY

UNIT I

Introduction to bioprocess: An overview of traditional and modern applications of biotechnological process, integrated bioprocess and the various (Upstream and down stream) unit operations involved in bioprocesses.

UNIT II

Fermentation processes: General requirements of fermentation processes, main parameters to be monitored and controlled in fermentation processes, aerobic an anaerobic

fermentation processes and their application in the biotechnology industry. UNIT III

Enzymatic bioconversion processes: Kinetics and thermodynamics of enzyme – catalyzed reactions, basic design and configuration of immobilized enzyme enzyme reactors,

applications of immobilized enzyme technology. Media design and sterilization for fermentation processes: Medium requirements for fermentation processes and for industrial

fermentation. UNIT IV

Metabolic stoichiometry and energetics: Stoichiometry of cell growth and product

fermentation, elemental balances, degrees of reduction of substrate and biomass, yield coefficients of biomass and product formation, maintenance coefficients energetic analysis of microbial growth and product formation.

UNIT V

Kinetics of microbial growth and product formation: Phases of cell growth in batch cultures,

simple unstructured kinetic models for microbial growth – Growth associated (primary) and non- growth associated (secondary) product formation kinetics – leudeking – Piret models.

BOOKS FOR STUDY

1. Shuler, M.L. and Kargi, F. 2002. Bioprocess engineering – Basic concepts. Prentice Hall of India.

2. Shuler, M.L. and Kargi, F. 1992. Bioprocess engineering, Prentice Hall. 3. Bailey and Ollis, 1986. Biochemical Engineering Fundamentals, McGrawHill (2nd Ed.).

83

616UBTP01 - GENETIC ENGINEERING AND BIOPROCESS (PRACTICAL)

GENETIC ENGINEERING

CO No

Course Outcomes

Knowledge Level

CO1

Students will acquire knowledge and perform hands

on experiments in the different gene transfer

techniques in genetic engineering.

K3

CO2

Students will get hands on experience in Bioprocess

production of biomass and its estimation.

K3

616UBTP01 - GENETIC ENGINEERING AND BIOPROCESS (PRACTICAL) GENETIC ENGINEERING

Extraction and estimation of intracellular proteins from E. Coli – Lowry’s Method – Production of competent cells for transformation – Bacterial transformation – Isolation of genomic DNA – Extraction and estimation of RNA – Restriction Digestion of DNA –

Absorption spectra of Nucleic acid – Estimation of DNA by Diphenyl amine method – Melting

temperature of DNA, Agarose gel electrophoresis – SDS – PAGE – Agrobacterium mediated

gene transfer – Isolation of plasmid DNA – Screening of Recombinants- Southern

hybridization (DEMO) – Western Blotting (DEMO) – DNA amplification – PCR (DEMO). BIOPROCESS

Bioprocess – Fermentor – Part and design, types of fermentor / Bioreactor – Prodection of Biomass and its estimation (dry weight) – Isolation and characterization of microorganisms

involved in biodegradation of amylolytic activity by DNS method – Compost making – Production of wine from grapes using bakers yeast – Production of alcohol by S. cerevisae –

Isolation of Rhizobial colonies involved in biofertilization – Isolation of lactic acid bacteria.

84

ELECTIVE II -616UBTT03 - MICROBIAL BIOTECHNOLOGY

CO No

Course Outcomes

Knowledge Level

CO1

Develop basic knowledge about the history and

scope of microbial biotechnology.

K1

CO2

Gain knowledge about the Production of microbial

enzymes and applications.

K2

CO3 Gain basic knowledge about the microbial

applications in food industries.

K1,K2


production and application of bio pesticides and

fertilizers.

K1,K2

CO5 Gain basic knowledge about different

bioremediation techniques.

K1,K2

616UBTT03 - MICROBIAL BIOTECHNOLOGY

UNIT I History and scope of microbial biotechnology, microbial diversity and its use, cultivation and

preservation of microorganisms in small scale in fermentors, bioreactors, immobilized cells

and microbial polysaccharides- Microbial Biomass

UNIT II Production of microbial enzymes and applications, production of organic solvents- single cell

proteins. UNIT III

Beverages Production of beverages, beer, wine, microbes in banking- production of baker yeast, milk products.

UNIT IV

Biofertilizers and Biopesticides, Biomass from carbohydrates, higher alkanes, methanol,

biofertilizers – manufacture, formulation and utilization, biopesticides. UNIT V

Bioremediation: Microbes in mining, ore leaching, oil recovery, waste water treatment,

biodegradation of non cellulose and cellulosic wastes for environmental conservation, protein.

85

BOOKS AND STUDY:

1. El-mans, E.M.T., and Bryce, C.F.A 2002. Fermentation microbiology and Biotechnology. Taylor and Francis group.

2. Prave, P., Faust, V., Sitting W. and Sukatseh, D.A. (eds.). 1987. Fundamentals of

Biotechnology. WCH Weinhein. 3. Moo-Young, M. (ed.) 1985. Comprehensive biotechnology - Volume 2,3 and 4.

Pergamon Press. 4. Stanbury, P.F. and Whitaker. A. 1984.Principles of fermentation Technology.

Pergamon Press. 5. Coulson, J.M. and Rocjardspm, J.F. 1984. Chemical Engineering. Pregamon press.

86

ELECTIVE III 616UBTT04 - ENVIRONMENTAL BIOTECHNOLOGY

CO No

Course Outcomes

Knowledge Level

CO1

Develop basic knowledge about the Biofilm

Kinetics.

K1

CO2

Gain knowledge about the design of anaerobic sludge

digesters.

K2

CO3 Gain basic knowledge about the microbial

Denitrification process.

K1,K2


Detoxification of Hazardous chemicals.

K1,K2

CO5 Gain basic knowledge about different forms of

sewage and waste treatment and its application in

different industries.

K1,K2

ELECTIVE III

616UBTT04 - ENVIRONMENTAL BIOTECHNOLOGY

UNIT I

Biofilm Kinetics: Soluble microbial products and inert biomass. Reactors: Reactors types – A batch reactor – A continuous- flow stirred- tank reactor with effluent recycle – A plug –

flow reactor – A Plug flow reactor with effluent recycles – Reactors with recycle of settled cells.

UNIT II

Linking stoichiometric equations to mass balance equations – Engineering design of reactors – Reactors in series. Reactor configurations – Special factors for the design of anaerobic sludge digesters.

UNIT III

Denitrification: Physiology of denitrifying bacteria – Tertiary denitrification – One – sludge

denitrification – Drinking water treatment: Anaerobic treatment by methanogenesis – Uses for methanogenic treatment.

UNIT IV

Detoxification of Hazardous chemicals: Factors causing molecular recalcitrance –

Biodegradations of problem environmental contaminants – Bioremediation of problem environmental contaminants – Bioremediation: Engineering strategies for Evaluating

bioremediation. UNIT V

87

Sewage and waste treatment: Pollution monitoring, control and remediation (petroleum

industry, paper industry, chemical industry etc). BOOKS FOR STUDY:

1. Rittmann, B.E. and McCarty, P.L. 2001. Environmental Biotechnology: Principles and

applications. McGraw – Hill, New York.

2. Ahmed, N. Qureshi, F.M. and Khan, O.Y. 2001. Industrial Environmental Biotechnology. Horizon press.

3. Smith, J.E. 1996. Biotechnology, 3rd edition. Cambridge Low price edition. Cambridge

University press. 4. Sohal, H.S and Srivastava, A.K. 1994. Environmental and Biotechnology, 1st edition.

Ashish Publishing House, New Delhi.

88

616UEAT01 - EXTENSION ACTIVITY

CO No

Course Outcomes

Knowledge Level

CO1

Students will acquire knowledge and provide service

and evaluate the current societal needs through survey

in extension activities through NSS /NCC/ NSO

(Sports & Games) Rotract/ Youth Red cross or any

other service organizations.

K5

616UEAT01 - EXTENSION ACTIVITY (COMMON TO ALL UG BRANCHES)

A candidate shall be awarded a maximum of 1 Credits for Complusory Extension Service.

All the Students shall have to enrol for NSS /NCC/ NSO (Sports & Games) Rotract/ Youth Red cross or any other service organizations in the college and shall have to put in

Complusory minimum attendance of 40 hours which shall be duly certified by the Principal

of the college before 31st March in a year. If a student LACKS 40 HOURS ATTENDANCE in the First year, he/she shall have to compensate the same during the subsequent years.

Students those who complete minimum attendance of 40 hours in One year will get HALF A

CREDIT and those who complete the attendance of 80 or more hours in Two Years will ONE

CREDIT. Literacy and population Education Field Work shall be compulsory components in the above

extension service activities.

89

Registrar

b.sc. (biotechnology) degree programme

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