botristop at abim 2013, premiere biocontrol event
TRANSCRIPT
“Early Stage and Demonstration trial results - Quillaja saponaria extract, effective against Botrytis cinerea in table grapes as an
integrated solution for grower´s Botrytis Management Program”
Paulina Sepulveda, MSc – INIA ChileABIM 2013
-Research Collaboration-
Agenda
• Chile in the World• Exports of Table Grapes• Disease: Grey Mold caused by Botrytis cinerea• Available Control Solutions• Our Solution• Experimental and Demostration trials • Summary
Field trials 2011-2012Botristop
Thompson Seedless GrapesAGRÍCOLA LONQUÉN
ISLA DE MAIPO, CHILE
Field trials 2011-2012
Early Stage
Treatment Products Dose Botristop Quillay extract (BS) 2L-HectareIntegrated Boscalid-Botristop-Fenexamid- Botristop-
Botristoplabel
Grower Boscalid- Fenexamid-Citric extract-Fenexamid label
Control -----
•Randomized Blocks•5 Treatments (4 plants)•3 Replications.
Early Stage
•5 sprays every 10 days•From veraison to harvest.
T1
T5
T2
T4
T3
T4
T2
T3
T1
T5
T1
T4
T5
T2
T3
• 25 bunches per plant• Incidence (%), • Severity (Scale 0-4) • Disease index• Phytotoxicity
Evaluation Methodology
Pre harvest
Incidence of grey mold
05-03-2012:
Treatments
Perc
enta
ge
cc
ab
a
Disease index of grey mold
05-03-2012:Di
seas
e in
dex
Treatments
b
ab
a
b
Field Trials 2012-2013
Demonstration
Thompson Seedless GrapesAGRÍCOLA LONQUÉN
ISLA DE MAIPO, CHILE
Demonstration
Treatment
Spraying machinery800L/hectare
Field test size:1 hectare
Data sampling:
H9 H13
BS400Control
H14 H23H18
Grower
H19
BS200
H29 H33H28
integrated
H24
Treatment Products Dose
Botristop Quillay extract (BS) 2L/Hectare
Integrated Boscalid–Botristop- Fenexamid-Botristop- Fenexamid-Botristop
label
Grower Boscalid- Fenexamid—Fenexamid- Citrus extract
label
Control No applications
4-6 sprays every 10 days from veraison to harvest
Evaluation Methodology
• 75 bunches per treatment• Incidence (%), • Severity (Scale 0-4) • Disease index• Toxicity
1- Pre harvest 2- Harvest
• Total weight of infected bunches
Incidence of grey mold
Incidence %, 27/02/2013
bb
a
b
Incidence of grey mold
Incidence %, 27/02/2013
a
b
a
98,25%
Infected grapes
Control Botristop Integrated Growerkg/plant 1,838 0,705 0,486 0,534
Fit pre harvest result
Avg. kilograms of damaged grapes per plant
Pos
Post HarvestEvaluation
• SO2• Stored fruit at 0°C (30-45 days)• Evaluation of infected berries
Post Harvest Results
Grapes stored at 0°C
Summary• Quillay extract (BS) represents a new alternative
to control Botrytis cinerea.
• BS effectively decreased the incidence of Botrytis cinerea when sprayed alone or alternated with chemical fungicides.
• BS did not caused phytotoxicity in any of the doses evaluated.
• Quillay production platform addresses consistency challenges of formulation´s active compounds.
“Early Stage and Demonstration trial results - Quillaja saponaria extract effective against Botrytis cinerea in table grapes as an
integrated solution for grower´s Botrytis Management Program”
-Thank You-
V