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Food Safety and Healthy Living – Book of Abstracts

BOOK OF ABSTRACTS

International Summer School

FOOD SAFETY AND HEALTHY LIVING

FSHL - 2019

June 30-July 3, 2019 Brasov, Romania

FOOD SAFETY AND HEALTHY LIVING FSHL – 2019


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Chairpersons

Mihaela BADEA, Transilvania University of Brasov, Romania

Laura FLOROIAN, Transilvania University of Brasov, Romania

Laura Elena GAMAN, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania

Patrizia RESTANI, Università degli Studi di Milano, Italy

Jean-Louis MARTY, University of Perpignan Via Domitia, France

International Scientific Committee

Valeriu ATANASIU, Carol Davila University of Medicine and Pharmacy, Bucharest

Mihaela BADEA, Transilvania University of Brasov, Romania

Doina Paula BALABAN, Ovidius University Constanta, Romania

Codruta Simona COBZAC, Babes-Bolyai Unversity of Cluj-Napoca, Romania

Gheorghe COMAN, Transilvania University of Brasov, Romania

Lorena DIMA, Transilvania University of Brasov, Romania

Dorin DRAGOS, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania

Laura FLOROIAN, Transilvania University of Brasov, Romania

Liviu GACEU, Transilvania University of Brasov, Romania

Laura Elena GAMAN, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania

Marilena GILCA, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania

Luis Alberto HENRÍQUEZ-HERNANDEZ, Universita Las Palmas de Gran Canaria, Spain

Viorel JINGA, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania

Jean Louis MARTY, University of Perpignan Via Domitia, France

Radovan METELKA, University of Pardubice, Czech Republic


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Marius MOGA, Transilvania University of Brasov, Romania

Corina MUSCUREL, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania

Gilvanda NUNES, University of Maranhao, São Luís, Maranhao, Brazil

Octavio PEREZ LUZARDO, Universita Las Palmas de Gran Canaria, Spain

Madalina PETRAN, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania

Lea POGACNIK, University of Ljubljana, Slovenia

Bogdan O. POPESCU, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania

Cristina POPOVICI, Technical University of Moldova, Moldova

Victor Lorin PURCAREA, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania

Mariana RADOI, Transilvania University of Brasov, Romania

Mugurel Petrinel RADOI, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania

Patrizia RESTANI, Universita degli Studi di Milano, Italy

Liliana ROGOZEA, Transilvania University of Brasov, Romania

Lluis SERRA-MAJEM, Universita Las Palmas de Gran Canaria, Spain

Mark SHAMTSYAN, St.Petersburg State Institute of Technology, Russian Federation

Rui SILVA, Universidade de Lisboa, Portugal

Irina STOIAN, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania

Polonca TREBSE, University of Ljubljana, University of Nova Gorica, Slovenia

Graziella TURDEAN, Babes-Bolyai Unversity of Cluj-Napoca, Romania


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FOOD SAFETY AND HEALTHY LIVING – FSHL 2019

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C1 Introduction in „FOOD SAFETY AND HEALTHY LIVING” Mihaela BADEA (1), Laura FLOROIAN (2), Laura GAMAN (3) 1-Faculty of Medicine, Transilvania University of Brasov, Romania 2-Faculty of Electrical Engineering and Computer Sciences, Transilvania University of Brasov, Romania 3-Faculty of Medicine, “Carol Davila” University of Medicine and Pharmacy Bucharest, Romania

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C2 Life sciences from the perspective of a nonlinear approach; theories, models, applications Florin MUNTEANU c.m. of Technical Sciences Academy of Romania

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C3 Oxidative stress induced by food contamination with metals Nicoleta TAUS Faculty of Medicine, Transilvania University of Brasov, Romania

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C4 The bacterial resistance to antibiotics – a serious global threat to public health Mihaela IDOMIR, Daniela NECULOIU Faculty of Medicine, Transilvania University of Brasov, Romania

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C5 Untapped potential of mushrooms for the well-being and for food industry Mark SHAMTSYAN Department of Technology of Microbiological Synthesses, Faculty of Chemical and Biotechnology, Saint Petersburg State Institute of

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Technology (Technical University), Saint Petersburg, Russia

C6 Mycotoxins – toxicity and detection in food matrices Jean Louis MARTY University of Perpignan via Domitia, France

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C7 Glucose monitoring using biosensors. Past-Present-Future Graziella TURDEAN Babes-Bolyai University of Cluj Napoca, Romania

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C8 Spectrophotometric analysis of medicinal plant extracts Simona Codruta COBZAC Babes-Bolyai University of Cluj Napoca, Romania

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C9 Biomonitoring of toxic chemical substances in Romanian population: serum levels of persistent organic pollutants and inorganic elements in adults and children Luis Alberto HENRÍQUEZ-HERNANDEZ, Universidad de Las Palmas de Gran Canaria, Spain

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C10 Ethical dilemmas and food safety attitude Liliana ROGOZEA Faculty of Medicine, Transilvania University of Brasov, Romania

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C11 The association between the Bisphenol A and polycystic ovary syndrome Marius MOGA, Florina ALDEA, Andreea BALAN, Oana DIMIENESCU, Calin COBELSCHI, Diana PANAIT Transilvania University of Brasov, Romania

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C12 The prevention of anaphylaxis in food allergy needs a multidisciplinary approach Patrizia RESTANI (1), Francesca COLOMBO(1), Simone BIELLA (1), Mihaela BADEA (2), Chiara

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DI LORENZO (1) 1 -Dept. of Pharmacological and Biomolecular Sciences, Università degli Studi di Milano, Italy 2 -Faculty of Medicine, Transilvania University of Brasov, Romania

C13 Polyphenols, from neuroprotection to safety Rui F.M. SILVA (1), Lea POGAČNIK (2) 1 -Research Institute for Medicines (iMed.ULisboa) and Department of Biochemistry and Human Biology (DBBH), Universidade de Lisboa, Lisbon, Portugal 2 -Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia

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C14 Invasive knotweeds – the source of bioactive substances Lea POGACNIK University of Ljubljana, Slovenia

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C15 In vitro models to evaluate the biological activity of plant-derived compounds Chiara DI LORENZO (1), Francesca COLOMBO (1), Simone BIELLA (1), Mario DELL’AGLI (1), Mihaela BADEA (2), Patrizia RESTANI (1) 1- Università degli Studi di Milano, Department of Pharmacological and Biomolecular Sciences, Milan, Italy 2 -Transilvania University of Brasov, Faculty of Medicine, Brasov, Romania

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C16 A highly sensitive xantine oxidase-based biosensor for the determination of antioxidant capacity in fruit samples Gilvanda S. NUNES (1), Magda M. BECKER (1,2), Jean-Louis MARTY (2), Gaelle CATANANTE (2) 1-Program in Biodiversity and Biotechnology, Bionorte, Federal University of Maranhão, Brazil 2-BAE-LBBM, University of Perpignan Via

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Domitia, France

C17 Recovery of bioactive components from fruit processing wastes Branka MOZETIC VODOPIVEC University of Nova Gorica, Slovenia

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C18 The role of genetic tests in weight loss Antoanela CURICI (1,2) 1-Faculty of Medicine, “Carol Davila” University of Medicine and Pharmacy Bucharest 2-Synevo Romania

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C19 Hygienic design for sustainability Liviu GACEU Faculty of Food and Tourism, Transilvania University of Brasov, Romania

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C20 Potential anticancer effects of food-related components Cristina POPOVICI Technical University of Moldova, Moldova

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C21 Nutrition in childhood - the premise for a healthy adulthood Bianca POPOVICI Faculty of Medicine, Transilvania University of Brasov, Romania

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C22 Accidental poisoning in children and chemical safety Elena Madalina PETRAN Faculty of Medicine, University of Medicine and Pharmacy Carol Davila, Bucharest, Romania

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C23 Cortisol, the alcohol of all our days Laura GAMAN Faculty of Medicine, University of Medicine and Pharmacy Carol Davila, Bucharest, Romania

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C24 The role of exposome in allergic diseases and asthma Ioana AGACHE Faculty of Medicine, Transilvania University of Brasov, Romania

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C1. Introduction in „FOOD SAFETY AND HEALTHY LIVING”

M. BADEA (1), L. FLOROIAN (2), L. GAMAN (3)

1-Faculty of Medicine, Transilvania University of Brasov,

Romania 2-Faculty of Electrical Engineering and Computer Sciences,

Transilvania University of Brasov, Romania 3-Faculty of Medicine, “Carol Davila” University of Medicine

and Pharmacy Bucharest, Romania

Started as an objective of a CEEPUS project -FOOD SAFETY FOR HEALTHY LIVING, the organization of this international summer school try to bring together the project partnership, but not only.

Transilvania University of Brasov is the CEEPUS project coordinator and valuable collaborators are involved also

„Carol Davila” University of Medicine and Pharmacy, Bucharest (Romania),

University Babes-Bolyai from Cluj-Napoca (Romania),

Faculty of Food Technology, Technical University of Moldova (Moldova),

Biotechnical Faculty, University of Ljubljana (Slovenia),

Faculty of Health Sciences, University of Ljubljana (Slovenia),

University of Rijeka (Croatia),

University of Nova Gorica (Slovenia),

University of Pardubice (Czech Republic),

University of Lódz (Poland),

University Ovidius Constanta (Romania).


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As co-coordinators of this event we have the mention University degli Study di Milano (Italy) and University of Perpignan (France).

We are happy to have with us academic staff with high visibility from Faculty of Pharmacy, University of Lisbon (Portugal), from St. Petersburg State Institute of Technology (Russia), from University Las Palmas de Gran Canaria (Spain), University of Maranhao, Sao Luis (Brazil) and from Center for Complexity Studies- (Romania).

The aim of this summer school is to disseminate multidisciplinary knowledge in new areas of food analysis using classic and modern techniques, linking environmental factors –food safety – nutrition - human health.

The main objective of the summer school FSHL 2019 is the education of undergraduate and postgraduate (master and PhD) students, coming from different institutions, teaching methodologies and pedagogical strategies for teaching/learning/evaluation in the field of food safety in order to have a healthy living.

Focused on subject areas of food control for a better life, the project identify topics as special monitoring of food composition (antioxidants), to correlate toxicological data (mycotoxins, heavy metals) with health status, to evaluate the possible methods and solution in order to increase the quality of life.

This international summer school “FOOD SAFETY FOR HEALTHY LIVING” will improve the inter-/multidisciplinary cooperation between higher education institutions in Europe, and not only.


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Mihaela BADEA

PhD (2005) in Chemistry - Babes-Bolyai University of Cluj-Napoca

Habilitation in Medicine, “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania

Professor of Biochemistry, Laboratory techniques, Organic chemistry, Methodology of scientific research at Faculty of Medicine, Transilvania University of Brasov, Romania

Coordinator of Clinical Laboratory, undergraduate specialisation (3 years), Faculty of Medicine, Transilvania University of Brasov

56 Nicolae Balcescu St., Brasov – 500019, Romania

Email – [email protected] [email protected]

Phone: (+040) 0725914533

ResearcherID: Z-1490-2018

URL: http://www.researcherid.com/rid/Z-1490-2018

ORCID: http://orcid.org/0000-0003-4824-2175 Research interests

Scientific and didactic activity demonstrated through articles and projects in the field of life sciences, international fellowships (in USA- University of North Carolina at Chapel Hill or financed by Balkan Environmental Association -B.EN.A) and research projects (CEEX projects for young researchers, FP7 project - Plant Food Supplements: Level of Intake, Benefit and Risk Assessment -PlantLIBRA no.245199).


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One of research aim is obtaining/optimization/application of enzyme biosensors with commercial acetylcholinesterase (Electric Eel) and mutants (Drosophila melanogaster) or immunosensors for detections of biologically active compounds (pesticides and mycotoxins) in different media: water, soil, plants, baby food, cereals, herbal supplements.

Recently the application of electrochemical detection was devoted to detection of other electroactive compounds with pro/antioxidant role.

Being in the same line with the most recent scientific studies of the moment (telediagnostic, telemonitoring systems, intelligent reconfigurable systems) and, in collaboration with groups from Faculty of IESC (UTBV), Dr. M. Badea conducted valuable scientific studies in this area, evidenced by completion of dissertation work for the master in medicine (2013), by coordinating an educational project for young master and PhD students (Erasmus Intensive Program -Telemonitoring and Telediagnosis for Life Sciences-12-EIP-E BRASOV01-BIS) and the organization in Brasov (July 24-26 ,2014) of the First International Conference on New Trends Sensing-Monitoring-Telediagnosis for Life Sciences (continued in 2015, 2017, 2018). Selected publications Badea M, Gaman L, Delia C, Ilea A, Leașu F, Henríquez-

Hernández LA, Luzardo OP, Rădoi M, Rogozea L., Trends of lipophilic, antioxidant and hematological parameters associated with conventional and electronic smoking habits in middle-age Romanians., J Clin Med. 2019 May 12;8(5). pii: E665

Miccoli A., Restani P., Floroian L., Taus N., Badea M.* , Cioca G., Bungau S., Sensitive electrochemical detection method of melatonin in food supplements, REV.CHIM. (Bucharest), 69, No. 4, 2018, 854-859


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Rauf S, Hayat Nawaz MA, Badea M, Marty JL, Hayat A, Nano-engineered biomimetic optical sensors for glucose monitoring in diabetes, Sensors 16 (11), 1931, 2016

Restani P., Di Lorenzo C., Garcia-Alvarez A., Badea M., Ceschi A., Egan B., Dima L., Lüde S., Maggi F.M., Marculescu A., Milà-Villarroel R., Raats M.M., Ribas-Barba L., Uusitalo L., Serra-Majem L., Adverse effects of plant food supplements self-reported by consumers in the plantlibra survey involving six European countries, PLoS One, 11(2) (2016) e0150089.

Badea M., Floroian L., Restani P., Cobzac SC., Moga M., Ochratoxin A Detection on Antibody- Immobilized on BSA-Functionalized Gold Electrodes, PLoS One., 11(7) (2016) e0160021.

Badea M, Luzardo OP, González-Antuña A, Zumbado M, Rogozea L, Floroian L, Alexandrescu D, Moga M, Gaman L, Radoi M, Boada LD, Henríquez-Hernández LA., Body burden of toxic metals and rare earth elements in non-smokers, cigarette smokers and electronic cigarette users., Environ Res. 2018;166:269-275.

Badea M., Braic M., Kiss A., Moga M., Pozna E., Pana I., Vladescu, A., Influence of Ag content on the antibacterial properties of SiC doped hydroxyapatite coatings, Ceramics International , 42(1) (2016) 1801-1811

Laura FLOROIAN PhD (2010) in Electronics,

Politehnica University of Bucharest

Associate Professor, Department of Automation and Information Technology

Faculty of Electrical Engineering and Computer

Science, Transilvania University of Brasov

5 Mihai Viteazu Str, Brasov, Romania

E-mail: [email protected]


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Phone: (+040) 0740163918

ORCID ID: 0000-0002-3289-8025 [https://orcid.org/0000-0002-3289-8025]

Research interests

Main research interests are on biomaterials fields, optical sensor for cell detection, biosensors for biological compounds and toxic compounds, advanced techniques for thin films deposition and advanced techniques for surface characterization. He is a member of many scientific societies: SRF – Romanian Society of Physics, Romanian Society of Automation and Technical Informatics (SRAIT), National Society for Medical Engineering and Biological Technology (SNMITB) and International Association of Online Engineering (IAOE).

Selected publications Badea M., Antuña A. G., Zumbado M., Rogozea L.,

Floroian L., Alexandrescu D., Moga M., Gaman L., Radoi M., Boada L. D, Henríquez-Hernández L. A., Body burden of toxic metals and rare earth elements in non-smokers, cigarette smokers and electronic cigarette users, Environmental Research, vol 166, pg. 269-275, 2018, ISSN: 0013-9351.

Floroian L., Ristoscu C., Candiani G., Pastori N., Moscatelli M., Mihailescu N., Negut I., Badea M., Gilca M., Chiesa R. and Mihailescu I.N., Antimicrobial thin films based on ayurvedic plants extracts embedded in a bioactive glass matrix, Applied Surface Science, vol 417, pg 224-234, 2017, ISSN: 0169-4332.

Floroian L., Ristoscu C., Mihailescu N., Negut I., Badea M., Ursutiu D., Chifiriuc M. C., Urzica I., Dyia H. M., Bleotu C., Mihailescu I N., Functionalized antimicrobial composite thin films printing for stainless steel implant coatings, Molecules, 2016, 21, pp. 740-758, ISSN: 1420-3049

Badea M., Floroian L., Restani P., Cobzac S.C., Moga M., Ochratoxin A Detection on Antibody- Immobilized on


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BSA-Functionalized Gold Electrodes, PLoS ONE 2016, 11(7): e0160021. doi:10.1371/journal.pone.0160021.

Floroian L., Samoila C, Badea M., Munteanu D., Ristoscu C., F. Sima, I. Negut, M. C. Chifiriuc, I. N. Mihailescu, Stainless steel surface biofunctionalization with PMMA-bioglass coatings: compositional, electrochemical corrosion studies and microbiological assay, Journal of Materials Science: Materials in Medicine, 2015, vol 26, pp. 195-209, ISSN: 0957-4530.


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C2. A non-linear approach of life sciences; theories, models, applications

Florin MUNTEANU

c.m. of Technical Sciences Academy of Romania

It is known that any method of analysis, any

technique for assessing the quality of a products due to the level of knowledge at a given moment, in short by the paradigm in which these methods and techniques have been created. This observation highlights the direct relationship between the level of knowledge and the concrete ways of applying and verifying this knowledge. When science development generates a leap of paradigm and technology makes it possible to apply practically the new conceptual framework, it is expected that substantial changes will occur in the capitalization of knowledge through the emergence of new materials, evaluation techniques and equipment. A non-linear approach of life sciences will initiate new definitions and perceptions of quality. In the last decades, such a paradigm leap was generated by:

The emergence and development of Complexity science (which allowed the study of nonlinear systems with hierarchical structure and evolution far from the thermodynamic equilibrium);

The new vision of the universe based on the information-energy-matter triad (which defines the existence of information interactions that ensure the morphogenesis and homeostasis of the biostructures interacting with the inorganic medium, characterized by the continuous increase of the entropy);


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The development of computational science (which allowed the modeling and simulation of complex phenomena, the extraction of information from large and heterogeneous data bases: data mining, artificial intelligence, deep learning).

This work aims to present a synthesis of these directions that characterize the new scientific vision on the phenomena and processes carried out in the Universe to stimulate creativity and innovation in the university environment, to define new research themes that can be approached in the bachelor, Masters and PhD themes.

Florin MUNTEANU

Born on March 27, 1952, in Braşov;

B.S. - Polytechnic University, 1976.

Doctorate in Sciences: Industrial Engineering;

Specialized in applied complexity science in engineering, econophysics, geodynamics and environment, as well as policies for integration into education of the informational and communicational technologies.

Member of the Academy of the Scientists from Romania, Chief Editor of Annals of the Academy of Romanian Scientists (ARS): Series: Earth Sciences.

Correspondent member of the Academy of Technical Sciences from Romania.

Founding President of the Center for Complexity Studies – UNESCO Center, the main promoter of the complexity science paradigm in Romania.

Founder of the youth training program for a scientific career in complexity science. NEXUS-T network, developed nationally, includes universities, high


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schools and secondary schools for the assurance of the continuity of the educational process, additionally with a special program of cognitive development and initiation, necessary for intuition development and for the abilities of integrating transdisciplinary the information from the surrounding environment.

Areas of specialty

Consultancy and mentoring for training in the human resources for knowledge based economy (mind building; transformational leadership).

Designing and production of the technical and conceptual framework for the transfer of knowledge and forming the specific abilities for working in interdisciplinary teams, capable to operate efficiently in a “stormy economy.

The coordination of the activities for continuous personal development through specific techniques for a continuous learning.


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C3. Oxidative stress induced by food contamination with metals

Nicoleta TAUS

Faculty of Medicine, Transilvania University of Brasov,

Romania

By means of water, soil, and during the processing or packaging, the food can be chemically contaminated with metals.

The purpose of this study is to carry out in the first part of the work, a synthesis of papers about contamination of the food with aluminium and cadmium, and in the second part of the paper is to reveal the presence of metals in the food and changes in oxidative stress induced by these.

One of the major mechanisms of toxicity associated with metals such as aluminium and cadmium has been attributed to the generation of reactive oxygen and nitrogen species.

Humans and animals can develop an imbalance between elements prooxidants and antioxidants elements in the body - a process called oxidative stress.

The oxidative stress induces liver damage (hepatotoxicity), to central nervous system (neurotoxicity), to DNA (genotoxic), may cause severe damage to bone and intestinal tract tissues, and to kidney.

The metals have capacity to induce free radicals that may cause lipid peroxidation, DNA damage, and protein oxidation.

Cadmium is one of the six substances incriminated RoHS (European Union's Restriction and Hazardous Substances) for cancer of the lung, prostate, pancreas and kidneys, and is classified in first place by carcinogenic Agency for Research on Cancer in the US.


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In 1912, the Food & Drug Administration in the US decided to exempt several substances from testing for food safety, including the aluminium, which was classified GRAS (Generally Regarded as Safe), so there has never been tested for safety and there no restriction on the amount and the use of aluminium.

After administration of large doses of aluminium hydroxide, have been reported, excessive aluminium deposits in the brain, liver, heart, and muscle. The first describes of the toxic effects of aluminium on the CNS, has been reported since 1921.

Subsequent studies have demonstrated the presence of aluminium in the onset of outbreaks susceptible to Alzheimer's disease.

The risk of food contamination by metals, was conducted by analyzing studies published in professional journals, and through a retrospective study conducted over a period of four years from the Sanitary Veterinary and Food Safety Brasov, which shows cadmium contamination of meat samples.

The changes caused by oxidative stress induced by aluminium was evidenced by dosing antioxidant enzymes, and by the pathological lesions studied to experimental animals.

Nicoleta TAUS

Medical degree “Carol Davila” University, Bucharest and absolved Magna Cum Laude her PhD in Clinical Pharmacology

Associate Professor of Pharmacology and Clinical Toxicology, at Faculty of Medicine, Transilvania University of Brasov, Romania

Department of Fundamental, Prophylactic and


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Clinical Disciplines Faculty of Medicine, Transilvania University of Brasov, Romania,


Email [email protected]

Phone: (+040) 072424627

Research interests

Main research interests are in the study of factors that influence oxidative stress.

We have analyzed the modifications induced by metals, drugs, plant extract with antioxidant and pro-oxidant action.

Another field is the use of biosensors for the detection of oxidative status under the influence of antiarrhythmic drugs, beta-blockers, or anticoagulants.

Selected publications Miccoli A., Restani P., Floroian L., Taus N., Badea M. ,

Cioca G. , Bungau S., Sensitive electrochemical detection method of melatonin in food supplements, REV.CHIM. (Bucharest), 69, No. 4, 2018, 854- 859.

Bodean OM,. Marcu RD, Spinu DA, Socea B, Diaconu CC, Munteanu O., Taus N., Monica Cirsto., Pelvic floor disorders in gynecological malignancies. An overlooked problem?, Journal of Mind and Medical Sciences, 2018; 5(1) 46

Floroian L., Calaver D., Manea A., Taus N., Badea M., Advanced antimicrobial thin coatings for dental implants functionalization. Problems, solutions and innovations in dental materials, 24th European Dental Materials Conference, London, 2017, Absract Booklet – 3-8 2017, p 35.

Taus N., Floroian L, Badea M., Enache D. V., Gherghel M., Farraj, M. , Oxidative stress induced by titanium implants, problems, solutions and innovations in dental materials, 24th European Dental Materials Conference, London, 2017, Absract Booklet – 3-8 2017, p 51.


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Taus N, Corcan O.E., Diclofenacum induce change in oxidative stress, New Trends on Sensing – Monitoring – Telediagnosis for Life Sciences- Brasov, Romania, September 3-5, 2015, pg 64.link:http://www.healthfoodenviron.unitbv.ro

Taus N, Pozna E., Marchitan C,. Antibacerial activity of essential oils of thyme, New Trends on Sensing – Monitoring – Telediagnosis for Life Sciences- Brasov, Romania, September 3-5, 2015, pg. 63.

Farraj M., Necula V., Taus N., Boicu M., Macri A., Mironescu A., The impact of aflatoxin and ochratoxin on animal organs - more experimental data, Environmental Science and Technology, 2014 vol 2, 176 -182. link:www.AASci.org/conference/env

Badea M., Pascu A.M., Nicolae R.A., Taus N., Nunes G.S. Moga M.A., Possible errors in electrochemical detection of blood glucose, In Sensing in Electroanalysis, Vol. 8 (K. Kalcher, R. Metelka, I. Švancara, K. Vytřas; Eds.), pp. 115-123. 2013/2014 University Press Centre, Pardubice, Czech Republic, ISBN 978-80-7395-783-4 (pdf) .

Taus N., Farraj M., Tanase S., Mironescu A., Boicu M.,

Necula V., Taus L.: Aluminium – a Chemical Neurotoxic

Agent, Bulletin of the Transilvania University of

Brasov, Vol. 6 (55), No. 2 – 2013, Series VI - Medical

Sciences, ISSN 2065-2216 (Print), ISSN 2065-2224 (CD-

ROM): 1-9.

Taus N., Moga M., Badea M., Bigiu N., Enache VD.,

Enache M, Taus L., Effects of pollen oxidases, Journal of

Environmental Protection and Ecology, Volume: 11 Issue

3 Pages: 1177 - 1182; Published: 2010.

Taus N., Badea M., Taus R., Scarneciu V, Environmental

tobacco smoke increases the incidence of asthma,

Journal of Environmental Protection and Ecology,

Volume: 9 Issue: 2 Pages: 316 - 322; Published: 2008.

Taus N., Badea M., Taus R., Prediger E, Indoors air

pollution and acute intoxication with carbon monoxide,

Journal of Environmental Protection and Ecology,

Volume: 9 Issue: 4 Pages:773 - 781; Published: 2008.


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C4. The bacterial resistance to antibiotics – a serious global threat to

public health

Mihaela IDOMIR, Daniela NECULOIU

Faculty of Medicine, Transilvania University of Brasov, Romania

The bacterial resistance to antibiotics represents a natural phenomenon that can be accelerated by the abusive and without discernment use of these substances in the therapy or prophylaxis of human diseases, agriculture or other economic sectors.

Consequences in human medicine are important consisting of increasing risk of death, increasing costs of medical care due to prolonged disease progression and infectivity, high risks associated with intensive care, major surgery, transplantation, therapy for premature babies or oncology therapy.

The mechanisms of bacterial resistance to antimicrobials are constitutive or induced by exposure to these drugs. For their in-depth understanding it is necessary to know the structure, physiology and molecular biology of bacteria, the mechanisms of action and the pharmacokinetics of antibiotics. The trends of the last decades consisted in the selection and spread of multi-resistant bacterial strains in contrast to the ever lesser development and introduction of new antibiotics. Nowadays, as signaled by the World Health Organization (WHO) since early 2014, antibiotic resistance has become a serious threat to global public health, with the risk of very high rates of morbidity and mortality by infections, similar to those before the era of antibiotics.


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Despite the political prioritization of antimicrobial resistance as a threat to public health, according to EARS-Net data, in many states in Europe, especially in the southern and eastern regions, including Romania, there are high levels of resistance of certain species to certain classes of antibiotics.

The optimization of the methods for detection of phenotypes of multiresistant bacteria of clinical and epidemiological significance, their confirmation by molecular biology methods, as well as the adoption of firm strategies limiting their spread in hospitals and in the community, is now an absolute priority throughout the globe.

Mihaela Elena IDOMIR PhD (2002) in Medicine,

Carol Davila University of Medicine and Pharmacy, Bucharest, Romania

Associate Professor of Bacteriology, Virusology, Parasitology, Department of Fundamental and Clinical Disciplines, Faculty of Medicine from Transilvania University of Brasov, Romania

Senior Doctor in Laboratory Medicine, Clinical County Emergency Hospital, Brasov, Romania

Member in the scientific editorial board of „Brasov Medical Journal” „Bulletin of the Transilvania University of Brasov”, member in the Romanian Society of Microbiology

56 Nicolae Balcescu Street, Brasov, 500019, Romania


Phone: (+040)0728958600


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Research Interests The main area of current interest refers to the

particularities of the etiological spectrum of nosocomial and communitary infections, antibiotic resistance patterns of the involved bacteria (Acinetobacter species, Pseudomonas aeruginosa, Klebsiella species, Escherichia coli, Providencia stuartii, Serratia marcescens, MRSA, VRE), the evaluation of phenotypes and genotypes of multidrug resistant strains implicated in nosocomial infections.

Other areas of my interest include antimicrobial resistance surveillance, the evaluation of changes of biological parameters associated with parasitic diseases (especially trichinellosis) or viral infections (infectious mononucleosis), biosensors in medicine, environmental pollutants detection. Publications

Books/ chapters: 13

ISI articles: 10, BDI articles: 24

Other medical journals articles: 129

Papers in conferences: 67

Selected publications

Idomir ME,The evaluation in dynamics of the antibiotic

resistance profile of Acinetobacter species in patients

from the intensive care unit, Jurnal Medical Brașovean,

nr. 2, 2018. pg. 34-39.

Idomir ME., Considerations on Involvement in

Hospitalized Patients and Antimicrobial Resistance of

Serratia species, Jurnal Medical Brașovean, nr. 2, 2017.

pg. 50-56.

Ionescu M., Mediavilla J. R., Chen L., Grigorescu D.,

Idomir M., Kreiswirth B., Roberts R. – Molecular

characterization and antibiotic susceptibility of

staphylococcus aureus from a multidisciplinary hospital in


26

Romania, Microbial Drug Resistance, vol. 16(4), 2010, pg.

263-272.

Nemet C., Dobrescu C., Bălescu A., Idomir M., Buvnariu

L., Rogozea L. – Retrospective epidemiological survey of

diagnostic errors and their implications in the

development of human trichinellosis in Braşov County-

Romania over a period of 10 years. Veterinary

parasitology, 194(2-4), 2013, pg.139

Bălescu A, Nemet C, Zamfir C, Ispas D, Idomir M.

Identifying risk factors for symptoms of severe

trichinellosis - a case study of 143 infected persons in

Brasov, Romania 2001-2008. Veterinary parasitology,

194(2-4), 2013, pg.142-144.

Taus N., Tărulescu S., Idomir M., Taus R. - Respiratory

exposure to air pollutants, Journal of Environmental

Protection and Ecology, vol. 9, no. 1, 2008, pg. 15-25.

Idomir M., Dima C., Nicolau A., Badea M., Taus N. –

Analysis of bacteriological al indicators of the water from

the source supply of Brasov, Journal of Environ-mental

Protection and Ecology, vol. 13, no. 3A, 2012, pg. 1881-

1886.

Idomir M., Idomir V., Repanovici A. - Web-Based

Application for Online Self-Testing and Knowledge

Evaluation in the Field of Microbiology, International

Journal of Education and Information Technologies, 2011,

5(2), pg. 141-148.

Bălescu A., Idomir M., Nemet C., Zamfir C., Ispas D.M. -

Clinical manifestations in human trichinellosis -

Retrospective epidemiological study, Program and

Abstract Book of 13th International Conference on

Trichinellosis, Changchun, China, 1st – 6th August, 2011,

p. 83.

Idomir M.E., Cocuz M.E., Cheşcă A., Nemet C. –

Analysis of Results and Optimization of a WEB-based

Program used for the Antibiotic Resistance Surveillance in

a Romanian Universitary Hospital, Proceedings of the


27

12th WSEAS international conference on Mathematical

and computational methods in science and engineering

(MACMESE'10), Faro, Portugalia, Nov. 3-5, 2010.

Cocuz M.E., Nemet C., Idomir M., Miclăuş R. - Software

guide suggestion to assist clinical medical diagnosis in

human trichinellosis in primary care, Proceedings of the

12th WSEAS international conference on Mathematical

and computational methods in science and engineering

(MACMESE'10), Faro, Portugalia, Nov. 3-5, 2010, pg.

164-168.

Idomir M.E., Rogozea L., Nemet C. – Web-services for

monitoring the resistance to antibiotics of pathogen

germs, Proceedings of the Applied Computing

Conference 2009 (ACC 09). Proceedings of the 11th

International Conference on Mathematical Methods and

Computation Techiques in Electrical engineering,

Vouliagmeni, Athens, Greece, WSEAS Press, 2009.


28

C5. Untapped potential of mushrooms for the well-being and for food industry

Mark SHAMTSYAN

Department of Technology of Microbiological Synthesses, Faculty of Chemical and Biotechnology, Saint Petersburg State Institute of Technology (Technical University), Saint

Petersburg, Russia

Mushrooms are a promicing source of variety of

biologically active compounds which can be used as pharmaceuticals, functional food supplements, plant protection products against pathogens and cosmeceutical preparations. According to approximate estimates, medicinal mushrooms have about 130 therapeutic functions. Mushrooms grow almlost everywhere and using available knowlegde and skills, growing of mushrooms may help improve nutrition security in different parts of the world. Among the biologically active substances released from mushrooms, special attention is given to enzymes, polysaccharides and polysaccharide-protein complexes. It is believed that among all fungal polysaccharides for the implementation of several activities, primarly β-glucans are respond.

Depending on the type of basidiomycetes, the structure of β-glucans is differently, what also leads to a change in its biological activity. Our studies demonstrate, that β-glucans of basidiomycetes have the following activities: Immunological, antitumour, antimicrobial, antiallergic, anti-inflammatory, anti- atherogenic, antiobesity, hypoglycemic, cardiovascular, antihypercholesterolemia and anti-diabetic effects. We also demonstrate, that mushroom beta-glucans can be successfully used to fortify common food products to give them additional functionality


29

Mark SHAMTSYAN

PhD (1997) in Biotechnology, St. Petersburg State Institute of Technology (Technical University)

Head of the Department of Technology of Microbiological Synthessis, Professor

St. Petersburg State Institute of Technology

Moskovsky prospect, 26, 190013, St. Petersburg,

Russia


Phone: (+7) 9602728168

GHI (Global Harmonization Initiative) Ambassador to Russia

Chair of Russian Section of EHEDG (European Hygienic Engineering and Design Group)

B-FoST (Black Sea Association of Food Science and Technology) ExCo member

Member of IAFoST (International Academy of Food Science and Technology)

Researcher ID: G-8618-2016

ORCID ID: 0000-0001-9824-2662

Scopus Author ID: 6504732524

Research interests

Main research are in the field of

biotechnology of production of biologically active

compounds, development of functional food products,

and influence of nutrition on health. Biologically active

compounds of fungal and bacterial origin, their

antioxiodant, immune modulative, antitumor,


30

antidiabetic, neuroprotective properties, as well as

influence on GUT microbiota and development of

functional food products or food supplements based on

these compounds.


Shamtsyan M., Konusova V., Maksimova Y.,

Goloshchev A., Panchenko A., Simbirtsev A.,

Petrishchev N., Denisova N.. Immunomodulating and

anti-tumor action of extracts of several mushrooms.

Journal of Biotechnology, 2004. V. 113 (1-3). 77-83.

Shamtsyan M., Bioactive compounds in mushrooms. In

Encyclopedia of Biotechnology in Agriculture and Food.

Edited by D. R. Heldman, D.G. Hoover, M.B. Wheeler.

Taylor & Francis, N.Y., 2010. P. 76-81.

Shamtsyan M., Vorobeychikov E., Konusova V.,

Simbirtsev A., Immunomodulating properties of higher

basidiomycetes mushrooms. Cytokines and

Inflammation. 2012. Vol. 11. № 1. P. 26–32.

Zukhurova M., Prosvirnina M., Daineko A.,

Simanenkova A., Petrishchev N., Sonin D., Galagudza

M., Shamtsyan M., Juneja L.R., Vlasov T.. L-theanine

Administration Results in Neuroprotection and Prevents

Glutamate Receptor Agonist-Mediated Injury in the Rat

Model of Cerebral Ischemia-Reperfusion. Phytother

Res. 2013 27(9):1282

Shamtsyan M., Dmitriyeva T., Kolesnikov B., Denisova

N.. Novel milk-clotting enzyme produced by Coprinus

lagopides basidial mushroom. LWT-Food Science and

Technology, 2014, 58 (2), 343–347

Shamtsyan M.. Food legislation and its harmonization

in Russia. Journal of the Science of Food and

Agriculture 2014, 94 (10), 1966–1969.

Shamtsyan M.. Potential to develop functional food

products from mushroom bioactive compounds. Journal


31

of Hygienic Engineering and Design, 2016, Vol. 15, pp.

51-59.

Shamtsyan M.. Mushroom Beta Glucans for

Development of Functional Food Products. Poceedings

of “Angel Kanchev” university of Ruse, Union of

scientists – Ruse – 2017. Reports Awarded with "Best

Paper" Crystal Prize. 2017 – Ruse, Silistra, Razgrad. P.

201-204

Antontceva E., Sorokin S., Krasnikova L., Shamtsyan

M.. Influence of Pleurotus ostreatus preparations on

fermentation products of lactic acid cultures. Journal of

Hygienic Engineering and Design V. 22, 2018, p. 47-52


32

C6. Mycotoxins – toxicity and detection in food matrices

Jean-Louis MARTY

University of Perpignan via Domitia, France

The analysis of foods to assess the presence of

chemical contaminants is a practice of crucial importance for ensuring food safety and quality. Over the past decade, the control of food safety has been mainly carried out through product testing rather than process control. The main problem with end-product testing is the high number of samples to be examined before one can decide on the safety of the product batch, especially when contaminants are expected to be heterogeneously distributed in the batch. The HACCP (Hazard Analysis Critical Control Point) system, which identifies specific hazards and measures for their control, is now generally accepted as the most effective system to ensure food safety.

Chemical contaminants can be present in foods and feed mainly as result of the use of agrochemicals, such as residues of pesticides and veterinary drug contamination from environmental sources (water, air and soil pollution), cross contamination or formation during the food processing, migration from food packaging materials, presence or contamination of natural toxins. The majority of chemical food contaminants are commonly analyzed using separative techniques coupled to various detectors such as GC-FID, GC-ECD, GC-MS, HPLC-UV, HPLC-FL, and HPLC-MS. Hence, the development of portable, rapid and sensitive detection method is essential for this purpose.

Natural toxins are defined as poisonous substances, which are synthesized by various


33

organisms as animals, certain plant species or by microorganisms. Due to their ubiquitous occurrence and the wide range of chemical structure of produced toxins, they represent an important economic and health risk. Among them, mycotoxins are toxic metabolites produced by fungi, mostly by saprophytic moulds on different foodstuffs. Additionally, they are also present in many plant pathogens. They have harmful effect on human and animal health. Mycotoxins are considered as harmful naturally occurring secondary metabolites [1]. Mycotoxin contamination has attracted the world wide attention, and has been considered as major financial problem due to the huge economic loses mainly based on human health, animal productivity, and national and international level trade [2].

The reported methods have some drawbacks such as high cost and less sensitivity etc. In order to address these issues, various methods have been developed. Among these, biosensors [3] are considered as promising tool in the assessment of mycotoxin food contamination. The presentation mainly focuses on the methods development in the sense that how mycotoxins can be detected through conventional analytical and new emerging bio-analytical approaches. References

[1] N.W. Turner, S. Subrahmanyam, S.A. Piletsky, Analytical methods for determination of mycotoxins: a review, Analytica Chimica Acta, 632 (2009) 168-180. [2] M. Vandeven, T. Whitaker, A. Slate, Statistical approach for risk assessment of aflatoxin sampling plan used by manufacturers for raw shelled peanuts, Journal of AOAC International, 85 (2002) 925-932. [3] B. Prieto-Simón, M. Campàs, Immunochemical tools for mycotoxin detection in food, Monatshefte für Chemie-Chemical Monthly, 140 (2009) 915-920.


34

Jean-Louis MARTY

Honorary Professor

Université de Perpignan Via Domitia 52 avenue Paul Alduy 66860, Perpignan Cedex France

Phone: +33 616814591

E-mail: [email protected] [email protected]

Co-founder of the company GTP Technology.

He has over 300 publications- including book chapters and text books.

He supervised 30 PhD with students from 12 nationalities.

He belongs to the Editorial Advisory Boards of some journals

Research Interest

Stabilization of enzymes and biomolecules and immobilization on various supports;

Development of enzyme sensors, immunosensors and aptasensors for the detection of pollutants in the environment; toxins and mycotoxins in food

Biosensors in F.I.A. systems,

Biosensors for agro-food and processing control

Studies of additives for neutraceutics and functional food

Analytical chemistry

Optical sensor

A new research interest beginning in 2016 is the smart packaging. The objective is to develop recyclable packaging with anti-oxidant and


35

antimicrobial activities. All the compounds used for the fabrication will be issue from bio-production


Khan R., Ben Aissa S., Sherazi T., Catanante G., Hayat

A., Marty JL, Development of an impedimetric aptasensor

for label free detection of patulin in apple juice,

Molecules 2019, 24(6), 1017

Majdinasab M., Mitsubayashi K., Marty JL, Optical and

Electrochemical Sensors and Biosensors for the

Detection of Quinolones, Trends in Biotechnology, 2019,

Goud K. Y, Kumar V S., Hayat A, K. Gobi V, Song H, Ki-

Hyun Kim, Marty JL, A highly sensitive electrochemical

immunosensor for zearalenone using screen-printed

disposable electrodes, Journal of Electroanalytical

Chemistry, 2019, 832, 336-342

Aissa SB, Mars A, Catanante G, Marty JL, Raouafi N.,

Design of a redox-active surface for ultrasensitive redox

capacitive aptasensing of aflatoxin M1 in milk,

Talanta, 2019, 195, 525-532

Nabok A., Al-Rubaye A. G., Al-Jawdah A. M.,

Tsargorodska A., Marty J.-L., Catanante G. , Szekacs

A., Takacs E., Novel optical biosensing technologies for

detection of mycotoxins, Optics and Laser Technology,

2019, 109, 212-221

Vasilescu A., Hayat A., Gaspar S., Marty J.L.,

Advantages of Carbon Nanomaterials in Electrochemical

Aptasensors for Food Analysis, 2018

Sharma A., Bueno D., Bhand S., Marty J.L., Muñoz R.,

Evaluation of various factors affecting fluorescence

emission behavior of Ochratoxin A: Effect of PH, Solvent

and Salt Composition, Biomedical Journal of scientific and

technical Research (BJSTR), 2018, 10, 4, 1-6

Majdinasab M., Hayat A. , Marty J.L., Aptamer-based

assays and aptasensors for detection of pathogenic


36

bacteria in food samples, TrAC - Trends in Analytical

Chemistry, 2018, 107, pp. 60-77

Al-Rubaye A.G. , Nabok A., Catanante G., Marty J.L.,

Takács E. , Székács A. , Label-free optical detection of

mycotoxins using specific aptamers immobilized on gold

nanostructures, Toxins, 2018, 10(7), 291

Sharma A., Khan R., Catanante G., Sherazi T. A., Bhand

S., Hayat A. , Marty J. L., Designed strategies for

fluorescence-based biosensors for the detection of

mycotoxins,Toxins 2018, 10, 197-215

Zejli H., Yugender Goud K., Marty J.L., Label free

aptasensor for ochratoxin A detection using

polythiophene-3 carboxylic acid, Talanta, 2018, 185, 513-

519

Al Rubaye A., Nabok A., Catanante G., Marty J.L, Takacs

E., Szekacs A., Detection of ochratoxin A in aptamer

assay using total internal reflection ellipsometry, Sensors

and Actuators B: Chemical, 2018, 263, 248-251

Rhouati A., Nasir M., Marty J-L., Hayat A., Photoinduced

discharge of electrons stored in a TiO2-MWCNT

composite to an analyte: application to the fluorometric

determination of hydrogen peroxide, glucose and aflatoxin

B1, Microchimica Acta, 2018, 185, 26,.

Rhouati A., Bulbul G., Latif U., Li Z., Hayat A., Marty J.L.,

Nano-aptasensing in mycotoxin analysis; recent updates

and progress, Toxins 2017, 9, 349-362

Goud K. Y., Hayat A., Satyanarayana M., Kumar V. S.,

Catanante G., Gobi K. V., Marty J.L., Aptamer-based

zearalenone assay basedon the use of a fluorescein

label and a functional graphene oxide as a quencher,

Microchim Acta (2017) 184:4401–4408

Vasilescu A., Marty J.L., Aptasensors, an analytical

solution for mycotoxins detection, Comprehensive

Analytical Chemistry, 2017, (77), 1-40

Goud K. Y., Hayat A., Catanante G., Satyanarayana M.,

Gobi K. V., Marty J.L., An electrochemical aptasensor


37

based on functionalized graphene oxide assisted

electrocatalytic signal amplification of methylene blue for

aflatoxin B1 detection, Electrochimica Acta 2017 (244)

96–103

Bueno D., Mishra R., Munoz R., Marty J-L., Low cost

optical device for detection of fluorescence from

Ochratoxin A using a CMOS sensor", Sensors &

Actuators: B. Chemical 2017, (246) 606–614

Bazin I., Tria S. A., Hayat A., Marty J-L., New

biorecognition molecules in biosensors for the detection of

toxins, Biosensors and Bioelectronics, 2016, 87, 285–298

Sharma A., Yugender Goud K, Hayat A., Bhand S., Marty

J-L., Recent advances in electrochemical-based sensing

platforms for aflatoxins detection, Chemosensors 2017, 5,

1-15.


38

C7. Glucose monitoring using biosensors.

Past-Present-Future

Graziella TURDEAN

Babes-Bolyai University of Cluj Napoca, Romania

Glucose is the main source of energy for cellular

activity in the living body; consequently the blood glucose concentration is a key indicator of patients’ health, particularly for symptoms associated with Diabetes mellitus.

Among many approaches existing for glucose measurements, electrochemical analysis using enzyme-based electrochemical glucose sensors is important, because it is simple and able of providing quantitative or semi-quantitative analytical information. Remarkable research for new concepts and sensor architectures has been made since 1967, when Updike and Hicks reported in their article the first enzyme based electrode for detection glucose [1]. Consequently, during the last 50 year, glucose monitoring market based on glucose sensors have been developed to exactly estimate the concentration of blood glucose and to assist the accurate delivery of the corresponding medication for homeostatic regulation [2].

A clear and concise view on the evolution of enzyme-based electrochemical glucose sensors, from the perspectives of materials, mode of sensing, device structure and designs fabrication processes, and system engineering, in order to provide insights into the currently available technology for blood glucose monitoring is presented.

The glucose sensors classification (generation I to III) based on the principles of the enzymatic glucose


39

reaction is comparatively discuss. Based on such principles, key considerations in the development of glucose monitoring systems are also presented. Considering the growing interest in painless and continuous monitoring of blood glucose, the systems for blood glucose monitoring and the latest progress in non-invasive methods are compared for an efficient next-generation diabetic care. References [1] Updike S. J., Hicks G. P., The Enzyme Electrode, Nature, 1967, 214, 986–988. [2] Bobrowski T, Schuhmann W., Long-term implantable glucose biosensors, Current Opinion in Electrochemistry 2018, 10, 112-119.

Graziella Liana TURDEAN

Associate professor at Chemical Engineering Department of “Babes-Bolyai” University (BBU) (Cluj-Napoca, Romania).

B.Sc. degree in Chemical Engineering in 1989 at “Babes-Bolyai” University (Cluj-Napoca, Romania)

M.Sc. degree in Science and Environmental Techniques in 1994 from University Paris XII-Val de Marne (Paris, France)

PhD degree in co-direction was obtained in 1997 at BBU in the field of Chemistry and at University Paris XII-Val de Marne (Paris, France) in the field of Sciences and Technique of Environment, with a thesis on “Amperometric biosensor for studying the inhibition of cholinesterases”.

Post-doc NATO-CNR stages at University “Tor Vergata” (Rome, Italy) and University “La Sapienza” (Rome, Italy) improved her professional career.


40

The habilitation degree was obtained in 2016 with the thesis entitled: “Bio/molecular information processing of the fascinating world of nanostructures: from micro- to “smart” electrodes”.

She has done short visits (1-3 weeks) at foreign universities as: University of Alcala de Henares (Spain), University of Pisa (Italy), University of Cagliari (Italy), Al Farabi Kazakh National University (Almaty, Kazakhstan), Polytechnic School of Chimborazo (Riobamba, Ecuador).

Research interests

materials for bio/electrochemical sensors,

nanotechnology and materials sciences,

electrochemical investigation techniques

applied bio/electroanalytical chemistry.

Publications

She published 54 scientific papers (44 papers in journals having ISI factor, and at 37 is first or corresponding author). The articles were cited (without self-citations) by 220 other papers. The participation at 83 international and 20 national conferences enriched her experience in validation of the research results. As teacher, she is author or co-author at 3 scholar books and 2 chapters of books. Also, she has experience as director of grants (4 national and 3 international) and as member in research teams (29 national and 3 international).


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C8. Spectrophotometric analysis of medicinal plant extracts

Simona Codruta COBZAC

Babes-Bolyai University of Cluj Napoca, Romania

In recent decades, medicinal herbs, as vegetal

material or in the form of hydroalcoholic extracts, glycerine macerates or volatile/ethereal oils, are increasingly used as remedies in phytotherapy or as food supplements. Their use for a particular purpose depends on their chemical composition and therefore it is necessary to identify the original plant material or the forgeries. For this purpose, the existing pharmacopoeias indicate the investigation of fingerprints obtained from chromatographic analyses such as GC, HPLC or TLC.

Considering that the UV-Vis spectrum of the hydroalcoholic extract is correlated with the composition of a herb, representing its specific fingerprint, this work aims to identify the medicinal plants based on their UV-Vis spectra and chemometric data interpretation. The influence of extraction experimental conditions and the herb provenience were also evaluated.

Hydroalcoholic extracts of 40 herbs of different provenience were obtained by maceration for 10 days. The normalised, standardised, derivative and unprocessed UV-Vis spectra were digitised in order to obtain numerical data. Data evaluation was perform by using multivariate exploratory techniques such as: cluster analysis (CA), factor analysis (FA) and principal component and classification analysis (PCCA).

All the extracts were obtained under standardised conditions. The spectra were acquired on 200-800nm range. Among the applied chemometric methods, K-mean cluster analysis reveals that the 500-200nm range contain all information needed for samples


42

classification/ identification. The best method for joining tree clustering was the Ward’s method as amalgamation rule and 1-Pearson r for distance measure. The best cluster classification/identification was obtained by using as row data the 4-th derivative order spectra.

Despite their provenience, the 40 analysed herbs

were classified according to their species. These results could be also used for the herb identification.

Simona Codruta Aurora COBZAC PhD (2001) in

Chemistry (Sample preparation), “Babes-Bolyai” University

Lecturer, Department of Chemistry,

Faculty of Chemistry and Chemical Engineering, “Babes-Bolyai” University

Arany Janos, 11, 400028, Cluj-Napoca, Romania


43


Phone: (+40) 264595388

Researcher ID: J-9250-2012

Research interests Main research interests are on the chromatographic analysis methods - Thin layer chromatography; Sample preparation methods for chromatographic analysis; Analysis of food samples, environment, pharmaceuticals, medicinal plants; Chemometric methods for data evaluation. Selected publications Casoni D, Olah N, Soran L, Cobzac S.C.A., Comparison

of different extraction techniques for the evaluation of polyphenols content in Summer savory extracts, Studia Universitatis Babes-Bolyai Chemia, 2017

Simion M, Cobzac S.C.A., Image Analysis Approaches to Improve the Thin Layer Chromatography – Chemometric-Based Investigations of Natural Extracts, Studia Universitatis Babes-Bolyai Chemia, 2017

Vlase L, Muntean D, Cobzac S.C.A., Filip L, Development and validation of an HPLC-UV method for determination of synthetic food colorants Revue Roumaine de Chimie, 2014

Pop D, Casoni D, Cobzac S.C.A.,, Tartrazine Determination from Mustard Sample by TLC-Photodensitometry and TLC-Digital Processing of Images, , JPC - Journal of Planar Chromatography - Modern TLC, 2012

Cobzac S.C.A., Casoni D, Fazakaş A.L, Sârbu C., Determination of food synthetic dyes in powders for jelly desserts using slit-scanning densitometry and image analysis methods, Journal of Liquid Chromatography & Related Technologies, 2012

Naşcu-Briciu R.D., Cobzac S.C.A., Baciu S., Optimum Ultrasound Assisted Extraction Conditions of Some Flavonoids from Green Tea Leaves. Control Quality of


44

Green Tea Product by TLC Fingerprinting, Analytical Letters, 2011

Cobzac S.C.A., Gocan S., Sample preparation for high performance liquid chromatography: Recent progress, Journal of Liquid Chromatography & Related Technologies, 2011


45

C9. Biomonitoring of toxic chemical substances in Romanian population:

serum levels of persistent organic pollutants and inorganic elements

in adults and children

Luis Alberto HENRÍQUEZ-HERNÁNDEZ (1), Mihaela BADEA (2), Corina Elena Delia(3), Manuel

ZUMBADO (1), Luis D. BOADA (1) Octavio Perez LUZARDO(1)

1 Universidad de Las Palmas de Gran Canaria, Department

of Clinical Sciences, Las Palmas de Gran Canaria, Spain 2 Transylvania University of Brasov, Faculty of Medicine,

Brasov, Romania 3 „Alessandrescu-Rusescu” National Institute for Mother and Child

Health, Bucharest, Romania

Persistent organochlorine pollutants (POPs) are a group of highly lipophilic substances resistant to degradation with capacity for bioaccumulation and biomagnification in the food chain. Although its use has been prohibited or limited for decades, POPs are often measured in human serum, adipose tissue or milk, soil, water, and food of animal or vegetable origin. Organohalogenated pollutants include organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (BDEs), and by extension polycyclic aromatic hydrocarbons (PAHs). In the other hand, inorganic elements are substances that are naturally found in the environment as part of the planet. Many of these elements are essential for living beings, and it is the amount of substance present in organisms that conditions their toxicity or deficiency.


46

The present study aimed to measure circulating levels of OCPs, PCBs, and BDEs one decade after the last available study published on the adult population of Romania. Additionally, we measured the circulating levels of 42 elements, including trace elements, elements in the ATSDR's priority pollutant list and rare earth elements (REE), in children from Romania.

We conducted a cross-sectional study that included 121 Romanian subjects and 89 Romanian children and adolescent who attended a medical consultation at the Alfred Rusescu Institute for Mother and Child Care (Bucharest, Romania) in the context of the National Program on malabsorption, malnutrition, and chronic diarrhea among children.

We measured the serum levels of 20 OCPs, 18 PCB congeners, 8 BDEs, and the 16 PAHs by gas chromatography coupled to mass spectrometry. We also determined the serum concentration levels of 43 elements, including the trace elements and other REE and minor elements (ME) considered “emerging pollutants”.

p,p'-DDE and β-HCH were detected in 100% and 62% of the series (median values = 2.1 and 0.5 μg/L); PCB-153 was detected in 77.7% of the subjects and PCB-28 showed the highest median concentration (0.5 μg/L); BDE-47 was detected at a low concentration in 11.6% of the individuals; phenanthrene and naphthalene were present in 98.3% and 38.8% of the serum samples (median values = 0.9 and 6.5 μg/L, respectively). Age was the main determinant of the total body burden of OCPs and PCBs. A positive correlation was observed between p,p'-DDE and age (Spearman Rho = 0.312, P < 0.0001), suggesting the prevalence of higher levels of pollutants at older ages.

Blood levels of elements included in the ATSDR's priority pollutant list and REE were low. The highest levels—those contained in the 95th percentile of


47

the distribution—were observed among children younger than six years. The sum of iron, selenium, barium, nickel, antimony, and cerium was positively associated with hemoglobin (Spearman rho = 0.217, P-value = 0.041), while the sum of copper, thallium, niobium, and tantalum was negatively associated (Spearman rho = -0.228, P-value = 0.032).

The present results showed a significant decrease in serum levels of p,p'-DDE and HCH in Romania -a country which has traditionally reported higher values than other Eastern European countries- as well as low levels of PCBs and BDEs. The presence of PAHs suggests an unknown source of exposure that deserves further investigation.

Given the inherent sensitivity of the child population, additional studies are needed to assess the effects of these elements on their health.

Luis Alberto HENRÍQUEZ HERNÁNDEZ

Ph.D. (2006) in Veterinary (Genomics and Molecular Biology), Universidad de Las Palmas de Gran Canaria

Associate Professor, Department of Clinical Sciences

Facultad de Ciencias de la Salud, Universidad de Las Palmas de Gran Canaria

Paseo Blas Cabrera Felipe s/n. CP 35.016, Las Palmas de Gran Canaria (Spain)


Phone: (+34) 928451461


48

Researcher ID: https://www.researchgate.net/profile/Luis_Alberto_Henriquez-Hernandez ORCID ID: orcid.org/0000-0003-3237-0316 Research interests

Environmental toxicology, persistent organochlorine pollutants, biomonitoring of human populations, level of contamination of food, inorganic elements, electronic waste, legal medicine, abuse drugs, ethanol in medico-legal autopsies, forensic sciences, toxicology. Publications years 2017-2019 Cabrera-Rodríguez R, Luzardo OP, Almeida-González M,

Boada LD, Zumbado M, Acosta-Dacal A, Rial-Berriel C, Henríquez-Hernández LA. Association between prenatal exposure to multiple persistent organic pollutants (POPs) and growth indicators in newborns. Environ Res. 2019 Apr;171:285-292.

Camacho M, Herrera A, Gómez M, Acosta-Dacal A, Martínez I, Henríquez-Hernández LA, Luzardo OP. Organic pollutants in marine plastic debris from Canary Islands beaches. Sci Total Environ. 2018 Dec 29;662:22-31.

Luzardo OP, Badea M, Zumbado M, Rogozea L, Floroian L, Ilea A, Moga M, Sechel G, Boada LD, Henríquez-Hernández LA. Body burden of organohalogenated pollutants and polycyclic aromatic hydrocarbons in Romanian population: Influence of age, gender, body mass index, and habitat. Sci Total Environ. 2019 Mar 15;656:709-716.

Zumbado M, Luzardo OP, Rodríguez-Hernández Á, Boada LD, Henríquez-Hernández LA. Differential exposure to 33 toxic elements through cigarette smoking, based on the type of tobacco and rolling paper used. Environ Res. 2019 Feb;169:368-376.

Ginés R, Camacho M, Henríquez-Hernández LA, Izquierdo M, Boada LD, Montero D, Robaina L, Zumbado


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M, Luzardo OP. Reduction of persistent and semi-persistent organic pollutants in fillets of farmed European seabass (Dicentrarchus labrax) fed low fish oil diets. Sci Total Environ. 2018 Dec 1;643:1239-1247.

Henríquez-Hernández LA, Luzardo OP, Boada LD, González-Antuña A, Domínguez-Bencomo AI, Zumbado M, Burillo-Putze G. Assessment of 22 inorganic elements in human amniotic fluid: a cross-sectional study conducted in Canary Islands (Spain). Int J Environ Health Res. 2019 Apr;29(2):130-139.

Badea M, Luzardo OP, González-Antuña A, Zumbado M, Rogozea L, Floroian L, Alexandrescu D, Moga M, Gaman L, Radoi M, Boada LD, Henríquez-Hernández LA. Body burden of toxic metals and rare earth elements in non-smokers, cigarette smokers and electronic cigarette users. Environ Res. 2018 Jun 13;166:269-275.

González-Antuña A, Camacho M, Henríquez-Hernández LA, Boada LD, Almeida-González M, Zumbado M, Luzardo OP. Simultaneous quantification of 49 elements associated to e-waste in human blood by ICP-MS for routine analysis. MethodsX. 2017 Oct 15;4:328-334.

Henríquez-Hernández LA, González-Antuña A, Boada LD, Carranza C, Pérez-Arellano JL, Almeida-González M, Camacho M, Zumbado M, Fernández-Fuertes F, Tapia-Martín M, Luzardo OP. Pattern of blood concentrations of 47 elements in two populations from the same geographical area but with different geological origin and lifestyles: Food Safety and Healthy Living – Book of Abstracts 118 Canary Islands (Spain) vs. Morocco. Sci Total Environ. 2018 Sep15;636:709-716.

Cabrera-Rodríguez R, Luzardo OP, González-Antuña A, Boada LD, Almeida-González M, Camacho M, Zumbado M, Acosta-Dacal AC, Rial-Berriel C, Henríquez-Hernández LA. Occurrence of 44 elements in human cord blood and their association with growth indicators in newborns. Environ Int. 2018 Jul;116:43-51.

Henríquez-Hernández LA, Boada LD, Carranza C, Pérez-Arellano JL, González-Antuña A, Camacho M, Almeida-González M, Zumbado M, Luzardo OP. Blood levels of toxic metals and rare earth elements commonly found in e-waste may exert subtle effects on hemoglobin


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concentration in sub-Saharan immigrants. Environ Int. 2017 Dec;109:20-28.

Henríquez-Hernández LA, Luzardo OP, Boada LD, Carranza C, Pérez Arellano JL, González-Antuña A, Almeida-González M, BarryRodríguez C, Zumbado M, Camacho M. Study of the influencing factors of the blood levels of toxic elements in Africans from 16 countries. Environ Pollut. 2017 Nov;230:817-828.

Henríquez-Hernández LA, Luzardo OP, Valerón PF, Zumbado M, Serra-Majem L, Camacho M, González-Antuña A, Boada LD. Persistent organic pollutants and risk of diabetes and obesity on healthy adults: Results from a cross-sectional study in Spain. Sci Total Environ. 2017 Dec 31;607-608:1096-1102.

Henríquez-Hernández LA, Luzardo OP, Zumbado M, Serra-Majem L, Valerón PF, Camacho M, Álvarez-Pérez J, Salas-Salvadó J, Boada LD. Determinants of increasing serum POPs in a population at high risk for cardiovascular disease. Results from the PREDIMEDCANARIAS study. Environ Res. 2017 Jul;156:477-484.

Octavio Pérez LUZARDO

VMD (1992), University of Zaragoza, Spain

PhD (1998), University of Las Palmas de Gran Canaria

Full Professor of Toxicology

Research Institute of Biomedical and Health Sciences (IUIBS), Universidad de Las Palmas de Gran Canaria

Paseo Blas Cabrera Felipe s/n. CP 35.016, Las Palmas de Gran Canaria (SPAIN)


Phone: (+34) 928451424

Researcher ID: https://www.researchgate.net/profile/Octavio_Luzardo


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ORCID ID: orcid.org/0000-0002-4153-3028 Brief curricular notes

Octavio Pérez Luzardo, VMD, PhD, has specialized in Analytical Toxicology with environmental applications, including food safety and risk analysis. He is the manager of the Clinical and Analytical Toxicology Laboratory at the Research Institute of Biomedical and Health Sciences (IUIBS) of Universidad de Las Palmas de Gran Canaria.

He has held various teaching positions for 25 years in the University, and in 2016 he has become Full Professor of Toxicology at the University of Las Palmas de Gran Canaria, with teaching duties in the Faculties of Medicine and Veterinary Medicine of this University, either in pregraduate and postgraduate programs. He is also Secretary of the Department of Clinical Sciences of this University.

He is the author of more than 150 publications with a total of 92 JCR publications (> 50% in Q1 journals) with more than 2500 citations, an H index of 27, and a i10 index of 54. He has written or edited several books or book chapters on topics. During these years he has played a prominent role in the field of environmental toxicology, developing numerous studies aimed at evaluating the presence of environmental contaminants in human, animal or environmental samples and evaluating their effects on living organisms. He is also a specialist in toxicological risk assessment and has developed several analytical methods, which have been fully validated and published in international journals. He has been the leading researcher of several national and European research projects. He is a member several excellence research networks, such as CIBER of Obesity and Nutrition of the Spanish Ministry of Health; European Raptor Biomonitoring Facility of the European Union; and the Human Biomonitoring Programme of the European Union.


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C10. Ethical dilemmas and food safety attitude

Liliana ROGOZEA


Romania

Food is style an important problem of our century, which concerns both rich and developing or poor countries, and is a concern not only at government level but also at individual level in all of these countries.

Even if there are countries where the discrepancy between people in access to basic nutrients is very high, declaratively any government speaks of some major concerns it has, considering it essential for a good governance to be able to provide water, food, access to education and to health system for all its citizens.

In accordance with the United Nations General Assembly decision starting with this year, 2019, June 7 will be proclaimed the World Food Safety Day, because, as Tom Heilandt said, "World Food Safety Day will be a chance for everyone to take time to think about something we often take for granted: food safety. "

In this context, economic development, encouraging governmental structures not only to check how food is produced and processed, but also to create / adopt a standard for healthy eating is essential to reduce the pathology induced by an incorrect diet. Certainly, the information we have at this time about the repercussions of a genetically modified diet, based on forced growth and last but not least on the development of synthetic food are not enough evaluate and grounded by the researchers.

The ethics of a healthy diet must be more than just having the right labels, which the population is able to understand and recognize. It must be an ethic of the


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future, concerned with the medium and long-term consequences on humanity.

The transition from analyzing the current impact to long-term analysis is important not only in former communist countries such as Romania but also in developed countries. The difference is that there are still different food quality standards in countries in different parts of the world. In this context, standards such as: The Codex Alimentarius or Halal standards have emerged, but these do not entirely solve the problem.

The next years will offer specialists the opportunity to help change attitudes and optics around its global food and safety.

Even if, over a long period, food safety is appears like a problem only for rich countries, in fact for developing or underdeveloped countries, it is also important and could represent an alternative for reducing the human pathology.

Liliana Marcela ROGOZEA

MD, Senior specialist in public Health, PhD in Medicine

Professor of Bioethics, History of Medicine and Health Promotion

Department of Fundamental, Prophylactic and Clinical Disciplines

Faculty of Medicine, Transilvania University of

Brasov

Vice-rector

56 Nicolae Bălcescu Str., Brașov 500019, Romania


Phone: (+40) 744656279


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Research interests

Ms. Liliana Rogozea, professor, MD, PhD, Vice-rector with public relation and promotion of university image. Her teaching and research fields are: counseling in ethics, health promotion, and human behaviour.

She has expertise in curriculum development, design of research methods in international project activities (e.g. Tempus S-JEP –12156 –97, Leonardo da Vinci RO/2006/97125/EX), member of MATRA Project „Reinforcement of the Integrated System of Extramural Mental Health Care Services in Three Counties in Romania” coordinator de: HealthNet-TPO M, September 2006, and in coordination with international co-operations, in conferences organizing. Other international project coordinate by prof Rogozea are: “RING – TransferRING Supports for Caregivers” (LLP-LDV-TOI-09-IT-0446), Health Rehabilitation through Physical Exercise (HARPE, Project no. 503202-LLP-1-2009-1-UK-ERASMUS-ECDSP), Healthy Europe through learning practice (HELP, LEONARDO DA VINCI 2011-1-GB2-LEO05-05499), ”Pervasive development disorders (Autism, Asperger Syndrome, ADHD)” (P_ASA LLP-LdV/VETPRO/2011/RO/309).

Also she is member of management team of “Equal opportunities for health: action for development” (CUAMM, OCI-NSA ED/2011/239-187). She has several publications abroad as well. She is a editor or co-editor of 2 medical journals and reviewer for few national and international medical journals.

Selected publications Roman, N., Tirziman, E., Sorea, D., Miclaus, R.,

Repanovici, A., Amaricai, E., & Rogozea, L. (2018). Ethical Dilemmas in the Interdisciplinary Approach to Informed Consent to Patients in Physiotherapy Services


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in Romania. Revista de Cercetare si Interventie Sociala, 63, 290.

ROGOZEA, Liliana, et al. Education, ethics and e-

Communication in medicine. In: Proceedings of the 8th

WSEAS international conference on Distance learning

and web engineering. Wisconsin/USA: World Scientific

and Engineering Academy and Society (WSEAS). 2008.

Rogozea, Liliana; Sechel, Gabriela; Fleancu, Andreea.

Ethical aspects in bioengineering research. In: WSEAS

International Conference. Proceedings. Mathematics and

Computers in Science and Engineering. World Scientific

and Engineering Academy and Society, 2009.

Purcaru, D., Preda, A., Popa, D., Moga, M. A., &

Rogozea, L. (2014). Informed consent: how much

awareness is there?. PloS one, 9(10), e110139.

Dima, L., Repanovici, A., Purcaru, D., & Rogozea, L.

(2014). Informed consent and e-communication in

medicine. Revista Romana de Bioetica, 12(2).

Rogozea, L. M., Diaconescu, D. E., Dinu, E. A., Badea,

O., Popa, D., Andreescu, O., & Leaşu, F. G. (2015).

Bioethical dilemmas in using animal in medical research.

Challenges and opportunities. Romanian journal of

morphology and embryology= Revue roumaine de

morphologie et embryologie, 56(3), 1227-1231.

Olimid, A. P., Rogozea, L. M., & Olimid, D. A. (2018).

Ethical approach to the genetic, biometric and health data

protection and processing in the new EU General Data

Protection Regulation (2018). Romanian journal of

morphology and embryology= Revue roumaine de

morphologie et embryologie, 59(2), 631-636.

Drugus, D., Leasu, F., Sechel, G., Repanovici, A.,

Azoicai, D., & Rogozea, L. (2015). Assessment of

personal qualities of healthcare professionals–

determining factor for the improvement of healthcare

services in the Romanian health system. The Medical-

Surgical Journal, 119(3), 843-848.


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C11. The association between the Bisphenol A and polycystic ovary

syndrome

Marius MOGA, Florina ALDEA, Andreea BALAN, Oana DIMIENESCU, Calin COBELSCHI, Diana

PANAIT

Transilvania University of Brasov, Romania

In the last decades, polycystic ovary syndrome (PCOS) is often found as the primary cause of infertility in young females. One of the triggers of this reproductive age endocrine disorder is recently proved to be the exposure to endocrine disrupting chemicals (EDC), especially Bisphenol A (BPA). BPA is considered to be a “weak-estrogen” interfering with the normal endocrine production of steroids. High concentration of BPA is observed both in serum and urine of females with PCOS and is responsible for the decline of ovarian reserve and poor oocyte quality by affecting the cellular division of the female gametes.

This systematic review is focused on the causalrelationship between BPA and PCOS, analyzing and summarizing experimental studies, case-control studies, case-reports,andmeta-analysis. After a systematic search on scientific engines like PubMed, CrossRef and Google Academic with key-words EDC, BPA, PCOS we identified 15 articles that fitted the point of interest of the present review.

The common objective of the selected studies is females diagnosed with PCOS. The women included in studies showed a higher prevalence of PCOS related to BPA at ages between 25 and 35, BMI in the 25-34 range, mostly nulliparous and with no record of abortion. First-morning urine sample and blood tests were performed to measure the concentration of BPA on


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selected patients, which was significantly higher in the PCOS groups versus control groups. There was also found an association between BPA and insulin resistance (r=0.273, P<0.05) and testosterone (r=0.192, P<0.05), respectively androstendione (r=0.257, P<0.05).

The results confirmed the implication of BPA in PCOS pathogenesis, sustained by increased ovarian androgen production and other hormonal and metabolic abnormalities. Key-words: endocrine disrupting chemicals, Bisphenol A, PCOS, reproductive disorders, steroid imbalance

Marius Alexandru MOGA Medical degree University of

Medicine and Pharmacy ‘Iuliu Hatieganu’, Cluj-Napoca, Romania and absolved 1st Class Honours as a Medical Doctor

PhD in Medicine (2004)- University of Medicine and Pharmacy of Bucharest

University Professor in the Department of Medical

and Surgical Specialties of the Faculty of Medicine, Transilvania University, Braşov Dean of Faculty of Medicine, Transilvania University, Braşov

Head of Obstetrics and Gynecology Clinic Department at the Clinic Hospital of Obstetrics and Gynecology „Dr. I.A. Sbârcea” Brasov


Phone: +40 722 54 80 49

ORCID ID: 0000-0002-2442-6917

Scopus: 24468455300

Research interests

Scientific and didactic activity demonstrated through a wide range of articles and projects in the field


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of life sciences, especially Obstetrics and Gynecology, and research projects (A phase 3, double blind, multicentre, randomised, placebo-controlled study to determine the efficacy and safety of SPL 7013 Gel (VivaGel) to prevent the recurrence of bacterial vaginosis- Principal Investigator; FP7 project - PlantLIBRA (Plant Food Supplements: Level of Intake, Benefit and Risk Assessment), etc.).

Main research interests are in the Obstetrics and Gynecology area, in the topics of gynecological diseases, normal fetal development and associated pregnancy-pathology. In the last years, the research area was extended, studying the mechanism of action of various natural products, which could be used for prophylactic purposes and as therapeutical agents in treating gynecological cancers. In addition, the Chinese Traditional Medicine, and other types of complementary and integrative medicine, these trends being a direction of development of medicine in the present era represent a new direction of the research activity. Selected publications Moga M., Obstetrics and gynecology, „Transilvania”

University Publishing House Brașov, 2010. ISBN: 978-973-598-010-8

Ciuca S., Badea M., Pozna E., Pana I., Kiss A., Floroian L., Semenescu A., Cotrut C.M., Moga M., Vladescu A., Evaluation of Ag containing hydroxyapatite coatings to the Candida albicans infection. Journal of Microbiological Methods. 2016, 125: 12-18. doi:10.1016/j.mimet.2016.03.016 (IF = 2,026 / 2014)

Moga M.A., Daniilidis A., Bigiu N.F., Andrei C., Dinas K., Festila D.G., Ganglioneuroblastoma during pregnancy – A rare case report. Clin. Exp. Obstet. Gynecol. XLIII (43), n. 2, 2016, pg. 265-267. doi: 10.12891/ceog2054.2016 (IF= 0,434 )

Moga M., Anastasiu C., Arvatescu C., Mironescu A., Congenital diaphragmatic hernia – a different postpartum


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outcome – case report. (1403/poster); In Proceedings (Book of Abstracts): ABSTRACTS of 11th World Congress of Perinatal Medicine. Moscow, June 19- 22, 2013 Published in Journal of Perinatal Medicine 2013, vol.41, suppl.1 ISSN:0936-174X , DOI 10.1515/jpm-2013-2002 (IF=1,425/2013)

Moga M., Arvatescu C., Anastasiu C., Ungureanu D., Mironescu A., Pascu A., Assessment of the differences between pH and lactate in combined samples of fetal scalp blood.. Journal of Perinatal Medicine. 43: 281, Oct 2015; Presented to the World Congress of Perinatal Medicine, 3-6 nov 2015, Madrid, Spain.; pag. 673

Moga M., Carp D., Sima RM., Ples L., A rare case of trophoblastic tumor with fallopian tube localization following ovarian stimulation. Journal of Surgical Sciences Vol.3 No.3, 2016, ISSN 2457-5364

Florina ALDEA Obstetrics and Gynecology

Resident Doctor since 2019, Clinical Hospital of Obstetrics and Gynecology “I.A. Sbarcea”, Brasov

36 George Baritiu St., Brasov – 500025, Romania

Email: [email protected]

Phone: (+40)752388556

Research Interests

The research interests in the Obstetrics and Gynecology domain covers maternal and fetal medicine concept and the impact of the external factors in pathogenesys of gynecological syndroms and diseases. Drawing attention to ethiopathogenic factors allows an overview of important reproductive health problems, as well as establishing the proper management suitable for the treatment and prevention of these conditions.


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Selected publications Moisei C., Sima R.M., Aldea F., Ples L., What the nature

can do when somebody wants to obtain a pregnancy? Case report and review of the literature. Maedica J Clin Med,Suppl. 2016, 11(202), 161.

Mehedintu C., Antonovici M., Teodorescu C., Berceanu C., Aldea F., Unilateral renal agenesia – Case presentation. Peri 2(1646)

Dimienescu O.G., Moga M.A., Aldea F., Anastasiu C.V., Arvatescu C.A., Balan A., Diagnostic si prognostic ecografic in infectiile cu CMV din sarcina – review. Gine Suppl 1, 2019, 23(1), 39.

Andreea BĂLAN

PhD Student in Pharmacology since November 2018, University Transilvania, Brasov

Associate Teacher, Department of Biochemistry, Faculty of Medicine, Transilvania University of Brasov


Email [email protected] [email protected]

Phone: (+40)769901194

ORCID ID: 0000-0002-2730-3074

Research Interests

The main interest in my research activity consist in the analysis of etiopathogenesis and new strategies of management of the pathologies that represent real public health problems, especially in the Obstetrics and Gynecology domain.

I manifest a special interest for gynecological cancers, carcinogenesis process, the factors involved in


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the progression to the malignancy and natural agents which may be used as therapeutical agents, such as polyphenols. Selected publications Moga M.A., Bălan A., Anastasiu C.V., Dimienescu O,G.,

Neculoiu CD, Gavriș C. An Overview on the Anticancer Activity of Azadirachta indica (Neem) in Gynecological Cancers. Int J Mol Sci. 2018, 19(12).

Moga M.A., Bălan A., Dimienescu OG, Dima L. Complementary/ alternative treatment in breast cancer: complement or crime? O ΓKO ΛOΓIKOI & ENΔOΣKOΠIKOI ΠPOBΛHMATIΣMOI (2019). ISBN 978-960-599-255-2

Moga M. A., Dimienescu O. G., Bălan A., Scîrneciu I., Barabas B., Ples L. (2018). Therapeutic Approaches of Botulinum Toxin in Gynecology. Toxins, 10(4), 169.

Moga, M.A., Bălan, A., Dimienescu, O.G., Burtea, V., Dragomir, R.M., Anastasiu, C.V. Circulating miRNAs as Biomarkers for Endometriosis and Endometriosis-Related Ovarian Cancer— An Overview. JCM. 2019

Moga, M.A., Dimienescu, O.G., Bigiu, N.F., Bălan, A., Ples, L. Postmenopausal vulvovaginal atrophy - a systematic review of a public health problem solved with CO2 Laser therapy. Archives of the Balkan Medical Union. 2018, Suppl 1, 53.

Oana Gabriela DIMIENESCU

PhD Student in Obstetrics and Gynecology since November 2014, University Transilvania, Brasov

Assistant – Department of Medical and Surgical Specialties, Faculty of Medicine, Transilvania University of Brasov, Romania



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Email [email protected]

Phone: +40-0268-412-185

ORCID ID: 0000-0001-9407-389X https://orcid.org/0000-0001-9407-389X Research interests

Main research interests are in the Obstetrics and Gynecology area, in the topics of gynecological cancers and carcinogenesis, associated to natural products, which might be used as complementary therapy.

Knowing the exact mechanism of the carcinogenesis and all the factors involved in this process, and also the properties of food natural products and food-borne molecules, either introduced in the regular diet or as additives or medicines, we could discover new management strategies for this incurable disease. Selected publications Moga M. A., Dimienescu O. G., Arvătescu C. A., Ifteni P.,

& Pleș L. (2018). Anticancer activity of toxins from bee and snake venom-an overview on ovarian cancer. Molecules, 23(3), 692.

Moga M. A., Dimienescu O. G., Arvatescu C. A., Mironescu A., Dracea L., Ples L. (2016). The role of natural polyphenols in the prevention and treatment of cervical cancer-an overview. Molecules, 21(8), 1055

Moga M.A., Bălan A., Anastasiu C.V., Dimienescu O.G., Neculoiu CD, Gavriș C. An Overview on the Anticancer Activity of Azadirachta indica (Neem) in Gynecological Cancers. Int J Mol Sci. 2018, 19(12).

Moga M.A., Balan A., Dimienescu O.G., Burtea V., Dragomir R.M., Anastasiu C.V. Circulating miRNAs as Biomarkers for Endometriosis and Endometriosis-Related Ovarian Cancer- An Overview. JCM. 2019

Dull A.M., Moga M.A., Dimienescu O.G., Sechel G., Burtea V., Anastasiu, C.V. Therapeutic approaches of resveratrol on endometriosis via anti-inflammatory and anti-angiogenic pathways. Molecules. 24(4), 667.


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Călin Pavel COBELSCHI Senior Clinical Lecturer,

Department of Medical and Surgical Specialities, Faculty of Medicine, Transilvania University of Brasov, Romania

Head of 2nd Clinic Dept of Surgery, County Emergency Clinic Hospital of Brasov

General Manager of the County Emergency Clinic Hospital of Brasov

Correspondence Address: County Emergency Clinic Hospital of Brasov, Calea Bucuresti, No .25-27, Brasov, Romania

Hospital telephone: 0368 320022

Mobile phones: 0724054654, 0752054654

e-mail adress: [email protected] Research interest

Project Manager Course (Certificate No. 5982 / 03.06.2010)

Course Manager of Environmental Management Systems (Certificate 833 / 05.02.2013)

National Conference of Surgery, 2nd Symposium of the Romanian Association of Hepato-Bilio-Pancreatic Surgery and Liver Transplantation, IXth Symposium and Postgraduate Course of the Romanian Section of IASGO with the title: "Actualities in Hepato-Bilio-Pancreatic Surgery and Transplant Hepatic "- Bucharest 18.-21-04-2007.

Twenty-third National Symposium with International Participation in Gastro Enterology, Digestive Endoscopy and Hepatology - Sibiu 14.-16.06.2007.

Medical Days of Brasov - 07.-08.12.2007

The XXIVth National Congress of Surgery-Eforie Nord, June 2008


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Congresses and Surgery Conferences, held in the country between 2009-2019

Selected publications Diaconu CC, Drăgoi CM, Bratu OG, Neagu TP, Pantea

Stoian A., Cobelschi PC, Nicolae AC, Iancu MA, Hainăroșie R., Stănescu AMA., Socea B., New approaches and perspectives for the pharmacological treatment of arterial hypertension, Farmacia Nr.3 -20018

Hogea M., Belecciu A., Pascu A.M., Poroch V., Cobelschi C., Misarca C., Surgical consideration in colon cancer management, Bulletin of the Transilvania University of Brasov, Seria VI, Vol. 11 (60), No. 1 – 2018

Nicorescu I., Udeanu DI, Pintilie L, Mititelu M, Moroșan E, Ioniță AC, Cobelschi CP, Arsene AL, Antimicrobial activity of a synthesized fluoroquinolone with potential therapeutic use, International Conference of Translational and Integrative Medicine – 2018

Velescu BS, Anuta V, Aldea A, Jinga M., Cobelschi PC, Zbârcea CE, Uivarosi V., Evaluation of protective effects of quercetin and vanadyl sulphate in alloxan induced diabetes model, Farmacia Vol 65, 2, 2017

Gavriş C., Dimienescu O., Baracan A., Neculoiu D, Cobelschi C., Risk of recurrent thrombosis related to antiphospholipid antibodies, soluble cd40l and p-selectin seric levels in patients with antiphospholipid syndrome, Archives of the Balkan Medical Union, vol 10 (59) No.1 – 2017

Arvătescu C.A., Dimienescu O.G., Casap S., Podaşcă C., Cobelschi C., Mironescu A., Congenital defects of the anterior abdominal wall and diaphragm, Bulletin of the Transilvania University of Brasov, vol 10 (59), nr 2 – 2017

Toader I, Cobelschi C, Mironescu A, Hogea M, Gheorghiu A, Maier A, Management of perineal wound post rectal amputation, Buletin of the Transilvania University of Brasov, vol. 10 (59), no. 2 – 2017

Cobelschi C, Hogea M, Panait D, Pascu A, Gavris C, Maier A., Ileocecal tuberculosis – case report, Buletin of The Transilvania University of Brasov series VI; Medical sciences vol 9 (58) no. 2 – 2016


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Elena Diana PANAIT Assistant Professor, Faculty of

Medicine, Transilvania University of Brasov


Phone: 0728587447

Graduation - Faculty of Medicine, Transilvania University of Brasov

56 Nicolae Balcescu St, Brasov, Romania

Research Interests Main research interests are on the biochemistry

area, in the topics of antioxidants, neurobiology, heavy metals neurotoxicity and general toxicity, anthropology and gynecology. Determining antioxidant properties of different biological fluids is the most important area of intervention as most of pathologies tend to bring this type of alterations to the human body. Selected publications Panait D., Jufa A., Florioan L., Pascu A., Badea M..

Microwave emit electromagnetic field: study of radiation leakage. Journal of Medicine and Life Vol. 10, Special Issue second edition, 2017 http://www.healthfoodenviron.unitbv.ro/2017/program/

Panait D., Maxim A., Martinescu C., Maier A., Bigiu N.. The evaluation of the incidence and characteristics of IUGR in the clinical hospital of obstetrics and gynecology between 2010-2014. Journal of Medicine and Life Vol. 10, Special Issue second edition, 2017

Panait D., Bigiu N., Martinescu C., Moga M., Pseudocholinesterase decrease following exposure to insecticides in a rural area cohort of Brasov county. - Bulletin of the Transilvania University of Brasov- Series VI : Medical Sciences, Vol 10(59). No 1 ;2017- trimis spre publicare

Panait D., Dima L., Coman Gh., Rogozea L., Badea M.. Studiul nivelului de cunoștințe privind vitamina C la


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studenți ai Facultății de Medicină, Jurnal Medical Brasovean, nr 1:37-42, 2017

Barbat O.A., Panait D., Martinescu C., Rogozea L., Badea M.. Quality of life in dyalisis patients-Brasov, 2016. Jurnal Medical Brasovean, nr 1:57-60, 2017

Panait D., Bigiu N., Martinescu C., Arvatescu C., Marcu V., Moga M.. The evolution of premature birth rate depending on socio-economic factors in Brasov county. Balkan Medical Union, vol 52, no 3, 249-253, 2017


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C12. The prevention of anaphylaxis in food allergy needs a multidisciplinary

approach

Patrizia RESTANI (1), Francesca COLOMBO(1), Simone BIELLA (1), Mihaela BADEA (2), Chiara DI

LORENZO (1)

1 -Dept. of Pharmacological and Biomolecular Sciences, Università degli Studi di Milano, Italy

2 -Faculty of Medicine, Transilvania University of Brasov, Romania

Starting from some facts of Italian news and from

the cases published in the scientific literature, we intend to make a review of those factors that can contribute to the onset of an anaphylactic phenomenon and make it more or less at risk for fatal event.

The risk that an anaphylactic shock evolves in a fatal event increases proportionally to the number of factors contributing to its induction and subsequent course.

Among the most important factors we should mention: 1) factors related to foods both from industrial production and collective catering (canteens or restaurants); 2) the behavior of the allergic subject that, in some cases, is not consistent with the severity of the allergic form.

In most cases, food industries are able to ensure a low cross-contamination rate, based on very selective and specific quality controls. On the other hand, the lack of legal limits related to "traces", pushes companies to indicate on the label "may contain traces of", "produced in a plant in which there are allergens ...". These sentences, that allow companies to protect themselves legally, create confusion and often lead allergic people to extreme behaviors: either they always ignore these


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warnings or exclude from the diet many products that could actually be consumed.

The situation in public area, such as canteens and restaurants, is even more complex. In this case the risk management depends exclusively on the personnel working in the structure, which must be specifically trained.

The risk of anaphylaxis, and in particular the fatal one, also depends very much on the allergic subject, who must be aware of his "sensitivity" and avoid the most critical situations, such as restaurants not known or with too much people at tables.

Finally, the pharmacological and social aspects must be well managed: auto-injectable epinephrine is the life-saving drug and must always be available to subjects with previous anaphylactic events; friends and family must be informed about the use of this device to rescue the allergic person in case of unconsciousness.

In cases, where anaphylaxis is associated with very small doses of allergens, oral desensitization at specialized hospitals would be advisable.

Patrizia RESTANI Dept. Pharmacological and

Biomolecular Sciences Università degli Studi di Milano, Italy Via Balzaretti 9 20133 Milano, Italy

1977-Doctor in Pharmaceutical Chemistry and

Technology (CTF) with the highest honours at the Università degli Studi di Milano, Italy


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1985-Post-graduation in Toxicology with the highest honours at the Università degli Studi di Milano, Italy.

2012-President of the course in Science and Safety of the Environment (SSCTA) at the Faculty of Drug Sciences (Scienze del Farmaco)

2016-Full Professor in Food Chemistry, Università degli Studi di Milano.

Teaching activities: Food Chemistry, Dietetic Products, Analytical methods for xenobiotic detection in foods, Food safety at the PhD course in “Nutrition and Health”, Dietetic Therapy at the Doctorate in “Nutritional Sciences”

National and international positions

Scientific Board of the Centre for “Caratterizzazione e sicurezza d'uso dei principi attivi di sostanze naturali [Characterization and safety of active compounds from natural origin]”, School of Pharmacy, State University of Milan

Collaboration to the Program for Continuous Education in Medicine of the Ministry of Health

Member of the delegation of the Italian Ministry of Agriculture at the International Organisation of Vine and Wine (OIV) from 2003 for:

- Food Safety (expert) - Wine and Health (expert) - Methods of analysis (expert)

President of the OIV Expert Group on Food Safety

Member of the Expert group of the Italian Ministry of Health for the Guidelines on “Food allergy”

Member of EFSA Focus Group on: o Allergenicity (GMO Panel) 2015-2016 as a

representative of the Member State Italy

Member of EFSA Working Groups on: o NUTRI Food Allergy 2015-2018 o Procedures under Article 8 of Regulation (EC)

No 1925/2006 (from 2016)


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Member of RENTIC (Register of Italian Certified Toxicologists) from 2003

Technical adviser and Expert witness for Italian Courts in relation to trials on food supplements or foods

Technical adviser for Ministry of Health for analytical control of food supplements containing botanicals

Technical adviser for Italian Antipoison Centres in relation to botanicals

Research programs managed as Project Leader

Municipality of Milan (2008-2009). Flagship project “Istituto dell’Alimentazione e della Salute [Institute of Food and Health]”: Molecular and clinical studies for the development of new approaches to the prevention, diagnosis and therapy of food allergy and intolerances

Ministry of Agriculture (2009-2010). Studies for the safety assessment for allergic subjects of wines fined with milk and egg proteins

PlantLIBRA (Plant Food Supplements: Level of Intake, Benefit and Risk Assessment) – EU project n. 245199 – 2010-2014

Selected publications Restani P., Uberti F., Ballabio C., Peñas E., Di Lorenzo

C., Danzi R., Tarantino C.. Assessment of the presence of allergenic residues in commercial wines fined with caseinates or egg white proteins. Proceedings of the XXXIV World Congress on Vine and Wine - Porto (Portugal), June 19-25, 2011. ID 50 - ISBN 978-989-20-2449-3.

Di Lorenzo C., Colombo F., Bavaresco L., Bosso A., Peres de Sousa L., Moro E., Restani P.. Evaluation of the phenolic profile and antioxidant activity of table grapes and unfermented derivatives. Proceedings of the XXXVII World Congress on Vine and Wine - Mendoza


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(Argentina), November 6-14, 2014. ID 2014-534 - ISBN: 979-10-91799-33-1.

Restani P. Adverse effects of plant food supplements: an update from PlantLIBRA survey. NUTRAfoods 15, 232-233, 2016. ISNN-1827-8590 (Print Edition); ISNN-2240-5291 (Electronic Edition).

Di Lorenzo C., Badea M., Colombo F., Orgiu F., Frigerio G., Pastor RF., Restani P.. Antioxidant activity of wine assessed by different in vitro methods. Proceedings of the 40th World Congress on Vine and Wine - Sofia (Bulgaria), May 29-June 2, 2017. BIO Web of Conferences 9, 04008. DOI: 10.105/bioconf/20170904008.

Dell'Agli M., Di Lorenzo C., Sangiovanni E., Williamson G., Meoni P., Restani P., Pieters R.. Benefits: tradition of use, experimental models and human studies to support health claims of botanicals. In: "Food supplements containing botanicals: benefits, side effects and regulatory aspects. The scientific inheritance of the EU Project PlantLIBRA". Ed.: P.Restani. Springer International Publishing AG, 2018, ISBN 978-3-319-62228-6, ISBN 978-3-319-62229-3 (eBook), DOI 10.1007/978-3-319-62229-3, pp. 117-139.

Di Lorenzo C., Dopter A., Vecchio S., Lüde S., Colombo F., Orgiu F., Restani P.. The other face of the moon: Side effects, interactions and molecules of concerns. In: "Food supplements containing botanicals: benefits, side effects and regulatory aspects. The scientific inheritance of the EU Project PlantLIBRA". Ed.: P.Restani. Springer International Publishing AG, 2018, ISBN 978-3-319-62228, pp.141-176.

Badea M., Boniglia C., Carratù B., Chizzola R., Franz C., Giammarioli S., Mosca M., Novak J., Steenkamp PA., Restani P.. Protocols for developing and testing methods applied to the quality control of botanicals. In: "Food supplements containing botanicals: benefits, side effects and regulatory aspects. The scientific inheritance of the EU Project PlantLIBRA". Ed.: P.Restani. Springer International Publishing AG, 2018, ISBN 978-3-319-62228, 197-208.

Di Lorenzo C., Ruzicka J., Colombo F., Orgiu F., Frigerio G., Novak J., Badea M., Restani P.,


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Classic/recommended methods and development of new methods to monitor phytochemical composition of plant food supplements and their content in active molecules. In: "Food supplements containing botanicals: benefits, side effects and regulatory aspects. The scientific inheritance of the EU Project PlantLIBRA". Ed.: P.Restani. Springer International Publishing AG, 2018, ISBN 978-3-319-62228, pp. 209-226.

Badea M., Floroian L., Marculescu A., Gaceu L., Moga M., Gaman L., Cobzac C., Chang Q., Xue J., Restani P. Classic/recommended methods and development of new methods to control residues and contaminants of botanicals. In: "Food supplements containing botanicals: benefits, side effects and regulatory aspects. The scientific inheritance of the EU Project PlantLIBRA". Ed.: P.Restani. Springer International Publishing AG, 2018, pp. 349-378.

Di Lorenzo C., Colombo F., Orgiu F., Frigerio G., Caruso D., Restani P.. Classic/recommended methods and development of new methods to control adulteration and counterfeits.In: "Food supplements containing botanicals: benefits, side effects and regulatory aspects. The scientific inheritance of the EU Project PlantLIBRA". Ed.: P.Restani. Springer International Publishing AG, 2018, pp. 379-394.

Restani P.. Food Supplements: Botanicals. In: Ferranti, P., Berry, E.M., Anderson, J.R. (Eds.), Encyclopedia of Food Security and Sustainability, vol. 2, pp. 414–417, 2019. Elsevier.


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C13. Polyphenols, from neuroprotection to safety

Rui F.M. SILVA (1), Lea POGAČNIK (2)

1 -Research Institute for Medicines (iMed.ULisboa) and

Department of Biochemistry and Human Biology (DBBH), Universidade de Lisboa, Lisbon, Portugal

2 -Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia

Polyphenols are naturally occurring

micronutrients that are present in many food sources like wine, green tea, grapes, vegetables, red fruits and coffee. It is generally accepted that most polyphenols are potent antioxidants and may also reveal inflammatory properties. Based on those properties, together with the relative abundance of those food products in human diet, many studies highlight their potential role in the prevention and treatment of various pathological conditions connected to oxidative stress and inflammation, like cancer, cardiovascular and neurodegenerative disorders, but also pollutant-induced cell damage.

Recent data expand the initial antioxidant-based mechanism of polyphenols action by showing that they are also able to modulate several cell-signalling pathways and mediators. A representative example of such actions is described by Patel et all (1) where the authors revise the pharmacological applications of curcumin in several diseases, as well as a wide range of pleiotropic actions in the modulation of cell signal molecules.

The proposed benefits of polyphenols, either as protective/prophylactic substances or as therapeutic molecules, may be achieved by the consumption of a natural polyphenol-enriched diet, by the use as food


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supplements or formulation as pharmaceutical drugs/nutraceuticals. It was also proved that the health effects of polyphenols depend on the amount consumed as well as on their bioavailability. However, the over consumption of polyphenols may raise safety concerns due to accumulation of high levels of these molecules in the organism, particularly if we consider the loose regulatory legislation regarding the commercialization and use of food supplements. We previously showed that high levels of quercetin and epigallocatechin gallate may lead to some neuronal demise in a primary neuron culture model. Furthermore, high levels of quercetin disrupted the barrier properties in a human blood-brain barrier culture cell model. Few studies are focused on the safe use of polyphenols for disease prevention and treatment, but they are paramount to further promote their use in human health.

In conclusion, polyphenols are promising molecules for preventive and therapeutic interventions in a wide range of pathological conditions, being cancer and dementia maybe the most impactful ones, but the relations between dosage, toxicity and safety must be well know and closely monitored. This work was supported by iMed.ULisboa, Fundação para a Ciência e Tecnologia (FCT), Portugal (UID/DTP/04138/2013) and Slovene Research Agency (P4-0121 and J7-4050), Slovene Human Resources Development and Scholarship Fund (11012-18/2012).

Patel SS, Acharya A, Ray RS, Agrawal R, Raghuwanshi R, Jain P. Cellular and molecular mechanisms of curcumin in prevention and treatment of disease. Crit Rev Food Sci Nutr 2019:1-53.


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Rui Fernando Marques DA SILVA

PhD (2001) in Pharmacy (Cell and Molecular Biology), Universidade de Lisboa

Assistant Professor, Department of Biochemistry and Human Biology

Head of Histology and of Neurobiology

Faculdade de Farmácia, Universidade de Lisboa Av. Prof. Gama Pinto, 1649-003 Lisboa, Portugal


Phone: (+351) 217946400 Ext. 14527 Researcher ID: G-1910-2011 ORCID ID: 0000-0002-0118-9357 Scopus: 36626089100

Research interests Main research interests are on the

Neurosciences area, in the topics of neurobiology, neurotoxicology, neurodevelopment and glial function associated to neurologic conditions and neurodegeneration. Neuroprotection mechanisms is the most relevant area of intervention, embracing the neuroprotective properties of food natural products and food-borne molecules, either introduced in the regular diet or as additives or medicines, by several cell and molecular mechanisms, beyond the traditional antioxidant properties described for food polyphenols. Selected publications Silva RFM and Pogačnik L. Food, polyphenols and

neuroprotection. Neural Regeneration Res 2017; 12: 582-3.

Garcia G, Nanni S, Figueira I, Ivanov I, McDougall GJ, Stewart D, Ferreira RB, Pinto P, Silva RF, Brites D, Santos CN. Bioaccessible (poly)phenol metabolites from


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raspberry protect neural cells from oxidative stress and attenuate microglia activation. Food Chem 2017; 15: 274-83.

Pogačnik L, Pirc K, Palmela I, Skrt M, Kim KS, Brites D, Brito MA, Ulrih NP, Silva RF. Potential for brain accessibility and analysis of stability of selected flavonoids in relation to neuroprotection in vitro. Brain Res 2016; 15: 17-26.

Silva RFM, Rodrigues CMP, Brites D. Rat cultured neuronal and glial cells respond differently to toxicity of unconjugated bilirubin. Pediatr Res 2002; 51: 535-41.

Silva RFM, Rodrigues CMP, Brites D. Bilirubin-induced apoptosis in cultured rat neural cells is aggravated by chenodeoxycholic acid but prevented by ursodeoxycholic acid. J Hepatol 2001; 34: 402-8.


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C14. Invasive knotweeds – the source of bioactive substances

Lea POGACNIK

University of Ljubljana, Slovenia

Knotweed is in Europe and America a highly invasive plant, originating from Asia. Three different taxons are identified in Europe, namely Japanese knotweed (Fallopia japonica), Sakhalin knotweed (Fallopia sachalinensis) and Bohemian knotweed (Fallopia x bohemica). The best-known among them is F. japonica that has been since ancient times used in traditional Chinese medicine for treatment of different kind of diseases (e.g. inflammatory diseases, hepatitis, tumors, burns and hyperlipidemia). Among tissues, the most commonly used are rhizomes for reducing fever, treatments of high blood pressure and body detoxification. Dried stems are used in alternative medicine for treatment of inflammatory diseases, hepatitis and diarrhea. Furthermore, in China and Japan, Itadori tea is a traditional herbal infusion used for treating heart disease.

Several studies have recently been performed to confirm that the Japanese knotweed extract possess several different bioactivities, namely antioxidant, antidiabetic, antiviral, antibacterial, and antimycotic activity. It was shown that many different bioactive components are responsible for this, among which stilbenes (e.g. emodin, resveratrol and resveratrol derivatives), anthraquinones, flavonoids (e.g. catechin, epicatechin) and lignins are the most predominant. Resveratrol, one of the most powerful antioxidants and recently also among the most studied polyphenols, is a naturally occurring polyphenol typically associated with grapes and red wine, but also abundantly present in


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knotweed. There are already a few supplements on the market with resveratrol originating from the rhizomes of Japanese knotweed.

However, much less studies have been performed on the other two knotweed species, particularly on the Bohemian knotweed, which is the most widely spread due to its highest invasivity among the three species. The reason can be also attributed to the fact that this species was created by the natural crossing of the other two taxons in Europe and appeared much later than the other two and it was many times also misclassified as Japanese knotweed.

The results of our recent studies confirmed antioxidant, antidiabetic, antimicrobial as well as anticancer activities of extracts obtained from different tissues (rhizomes, leaves, stems and flowers) of all three knotweed species.

This work was supported by Slovene Research Agency (P4-0121).

Lea POGAČNIK PhD (2001) in Chemistry

(Biochemistry), University of Ljubljana,

Faculty of Chemistry and Chemical Technology, Slovenia

University of Ljubljana, Biotechnical faculty, Department of Food Science and Technology

Jamnikarjeva 101, Ljubljana, Slovenia


Phone: (+386) 13203781

ORCID ID: 0000-0003-1008-0633 http://splet02.izum.si/cobiss/bibliography?code=15650


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Research interests preparation and characterisation of cianobacteria

species Arthrospira platensis extracts before and after the lactic acid fermentation

preparation and evaluation of bioactivities in extracts of different tissues of alien knotweed species, namely Japanese knotweed (Fallopia japonica), Giant knotweed (F. sachalinensis) and their interspecific hybrid – Bohemian knotweed (F. x bohemica)

evaluation of brain accessibility and neuroprotection of different polyphenols, namely quercetin, epigallocatechin gallate (EGCG), cyanidin-3-glucoside (C3G), and nicotine

simulation of digestion and evaluation of the stability of pomegranate juice anthocyanins

Selected publications Silva RF.M., Pogačnik L. Food, polyphenols and

neuroprotection. Neural Regeneration Research, ISSN 1673-5374, Apr. 2017, vol. 12, iss. 4, p. 582-583

Pogačnik L., Poklar Ulrih N,. Invasive knotweed species as a rich source of antioxidants. Journal of EcoAgroTourism, 2018, vol. 14, no. 1, p. 5-10

Pogačnik L., Rogelj A., Poklar Ulrih N.. Chemiluminescence method for evaluation of antioxidant capacities of different Invasive knotweed species. Analytical letters, 2016, vol. 49, no. 3, str. 350-363

Pogačnik L, Pirc K, Palmela I, Skrt M, Kim KS, Brites D, Brito MA, Ulrih NP, Silva RF. Potential for brain accessibility and analysis of stability of selected flavonoids in relation to neuroprotection in vitro. Brain research, 2016, vol. 1651, p. 17-26,

Pirc K, Škarabot M, Pogačnik L., Žerovnik E, Poklar Ulrih N., The effect of tyrosine residues on [alpha]-synuclein fibrillation. Acta chimica slovenica, ISSN 1318-0207. [Tiskana izd.], 2015, vol. 62, no. 1, p. 181-189

Pogačnik L., Poklar Ulrih N., Application of optimized chemiluminescence assay for determination of the antioxidant capacity of herbal extracts. Luminescence, 2012, vol. 27, no. 6, p. 505-510


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C15. In vitro models to evaluate the biological activity of plant-derived

compounds

Chiara DI LORENZO (1), Francesca COLOMBO (1), Simone BIELLA (1),

Mario DELL’AGLI (1), Mihaela BADEA (2), Patrizia RESTANI (1)

1- Università degli Studi di Milano, Department of

Pharmacological and Biomolecular Sciences, Milan, Italy

2 -Transilvania University of Brasov, Faculty of Medicine, Brasov, Romania

Botanicals and botanical preparations are widely consumed all over the world. They represent a source of phytochemicals, which have received increasing attention for their potential health-promoting effects. The assessment of beneficial effects to support a nutritional claim can be affected by different parameters; among others, the biological variability of plant materials (including different parts such as leaves, seeds, etc.) and the concentration of active molecules are particularly critical factors. Even though human studies are the most suitable approaches to correlate the intake of a Plant Food Supplement with the improvement of physiological parameters, in vitro approaches can be considered a preliminary and necessary starting point to check their efficacy. In addition, in vitro assays could provide information about the possible mechanisms of action of the active compounds1.

Among the numerous targets of botanical active compounds, oxidative stress and inflammation are good candidates, since they are factors frequently involved in the induction of several pathological conditions. For


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example, gastric inflammation represents the most common gastrointestinal disorder, where free radicals and oxidative stress produced by cytokine release can play a role.

In this presentation, in vitro approaches will be described for a suitable evaluation of botanical antioxidant and anti-inflammatory activity; particular attention will be payed to Camellia sinensis L. and Vitis vinifera L. extracts. Examples will be illustrated on the application of spectrophotometric, chromatographic and biochemical tests and techniques (e.g. DPPH, ORAC, High Performance Thin Layer Chromatography, biosensors) and biological assays (e.g. NF-kB assay). These methods will be critically compared with other experimental approaches described in the literature, and biomarkers most frequently affected by plant-derived compounds considered. M. Dell’Agli, C. Di Lorenzo, E. Sangiovanni, G. Williamson, P.

Meoni, P. Restani, R. Pieters. Chapter 4. In: Food supplements containing botanicals: benefits, side effects and regulatory aspects, Editor P. Restani, Eds Springer, 2018.

Chiara DI LORENZO

Graduation in Pharmacy at Faculty of Pharmacy, Università degli Studi di Milano (2004)

PhD in Pharmaco-Toxicological, Farmacognostic and Biotechnologial Sciences at the Faculty of Pharmacy, Università degli Studi di Milano (2013)

Post-graduation in Hospital Pharmacy at the Università degli studi di Milano (2008)

Post-doctoral fellowship at the Laboratory of Food Chemistry and Toxicology (responsible: Prof. Patrizia Restani), Dipartimento di Scienze Farmacologiche e Biomolecolari, Faculty of


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Pharmacy, Università degli Studi di Milano (2013-2017)

Researcher in Food Chemistry and Toxicology, Dipartimento di Scienze Farmacologiche e Biomolecolari, Faculty of Pharmacy, Università degli Studi di Milano (from 2017)


Phone: (+39) 0250318274

ORCID ID: 0000-0002-3289-8025 Research Interests

The main area of research includes: quality

control of plant food supplements, development of

analytical methods for the detection of active

substances and xenobiotics in foods and plant food

supplements. The main analytical techniques used are

chromatography (HPLC, TLC, HPTLC, GC) for the

characterization of food and food supplements;

electrophoresis applied to food analysis and dietetic

products; immunoenzymatic tecniques (immunoblotting

and ELISA) for the detection of food allergens in

complex food matrices.

Chiara Di Lorenzo is involved in numerous

national and international research programs in the field

of Food Safety, Dietetic products, Risk and Benefit

Assessment; the most important was the European

Project PlantLIBRA (Plant Food Supplements: Level of

Intake, Benefit and Risk Assessment) in the context of

the 7th EU Framework Program, involving 25 partners

distributed in 4 continents.

Chiara Di Lorenzo is responsible of the

laboratory activities at the courses of Food Chemistry

(Chemical Safety and Toxicological Environmental

Sciences, Faculty of Pharmacy, Università degli Studi di

Milano); Chiara Di Lorenzo holds seminars at: the

course of Dietetic Products (Faculty of Pharmacy,


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Università degli Studi di Milano); analytical methods for

xenobiotic detection in foods (training activities); Dietetic

Therapy/Food Supplements (Doctorate in Nutritional

Sciences), and is thesis assistant at the Faculty of

Pharmacy, Università degli Studi di Milano.

She is a member of the Italian Delegation

(Ministry of Agriculture) at the OIV (International

Organization of Vine and Wine) in the Group of Experts

"Consumption, Nutrition and Health".

Chiara Di Lorenzo is co-author of 29 international

peer-reviewed publications and attended as speaker to

17 scientific congresses.


Di Lorenzo C., Dell’Agli M., Colombo E., Restani P..

Metabolic syndrome and inflammation: a critical review of

in vitro and clinical approaches for benefit assessment of

plant food supplements. Evid Based Complement Alternat

Med. (2013) doi: 10.1155/2013/782461.

Di Lorenzo C., Dell’Agli M., Sangiovanni E., Dos Santos

A., Uberti F., Moro E., Bosisio E., Restani P.. Correlation

between catechin content and NF-B inhibition by

infusions of green and black tea. Plant Foods Hum Nutr

(2013) 68:149-54. doi: 10.1007/s11130-013-0354-0.

Sangiovanni E., Di Lorenzo C., Colombo E., Colombo F.,

Fumagalli M., Frigerio G., Restani P., Dell'Agli M.. The

effect of in vitro gastrointestinal digestion on the anti-

inflammatory activity of Vitis vinifera L. leaves. Food

Funct. (2015) 6:2453-63 doi: 10.1039/c5fo00410a.

Di Lorenzo C., Ruzicka J., Colombo F., Orgiu F., Frigerio

G., Novak J., Badea M., Restani P..,

Classic/recommended methods and development of new

methods to monitor phytochemical composition of plant

food supplements and their content in active molecules.

In: Food Supplements Containing Botanicals: Benefits,

Side Effects and Regulatory Aspects: The Scientific


84

Inheritance of the EU Project PlantLIBRA, pp. 209-226.

10.1007/978-3-319-62229-3_8.

Colombo F., Di Lorenzo C. (corresponding author),

Regazzoni L., Fumagalli M., Sangiovanni E., Peres de

Sousa L., Bavaresco L., Tomasi D., Bosso A., Aldini G.,

Restani P., Dell’Agli M.. Phenolic profile and anti-

inflammatory activity of sixteen Table Grape (Vitis vinifera

L.) varieties. Food & Function, 2019 (in press) DOI:

10.1039/c8fo02175a.


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C16. A highly sensitive xantine oxidase-based biosensor for the determination of

antioxidant capacity in fruit samples

Gilvanda S. NUNES (1), Magda M. BECKER (1,2),

Jean-Louis MARTY (2), Gaelle CATANANTE (2)

1-Program in Biodiversity and Biotechnology, Bionorte, Federal University of Maranhão, Brazil

2-BAE-LBBM, University of Perpignan Via Domitia, France

In view of the protection provided by antioxidants, the use of these compounds in nutraceutical, medicinal, therapeutic, foods and cosmetic applications have been extensively reported. Because of this, development of analytical tests for antioxidant capacity has received much attention. In general, spectrophotometric, electrochemical and chromatographic methods have been explored for this purpose. However, in the last two decades, electrochemical biosensors have been considered an efficient alternative for measuring the antioxidant capacity in foods, presenting a higher performance to conventional assays, as well as higher biological relevance.

This chapter describes the development of an amperometric biosensor using Prussian Blue (PB) modified electrodes containing xanthine oxidase (XOD) enzyme immobilized by photopolymerization into an azide-unit pendant water-soluble photopolymer (PVA-AWP) and the optimization of its biofunctionalization process by chemometric modelling. A three-channel screen-printed electrode was used as transducer.

The research focuses on (1) the optimization of the biofunctionalization conditions of XOD enzyme such as PVA/PWA ratio (RXP), neon time irradiation time


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(TP), and XOD charge (CXOD), (2) the evaluation of biosensor performance, and (3) the detection of the antioxidant capacity of standard antioxidant substances and fruit samples. Operational conditions for electrode preparation were: XOD:PVA/AWP ratio of 1:2; exposure time to light of 30 min; pH = 7.5 at room temperature and enzymatic charge of 8 mU per electrode. The biosensors are stable, fast, simple, selective, cost-effective and sensitive, with respectively detection and quantification limits for antioxidants of 2.17, and 7.15 µM.

The applicability of this biosensor was demonstrated by in vitro analysis of gallic acid as standard antioxidant and Amazonian fruits as natural sources.

Figure. Surface diagram for the current response depending on RXP and CXOD (A), TP and CXOD (B), TP and RXP (C).

Gilvanda Silva NUNES is Industrial Chemistry by the Federal University of Maranhão (UFMA, 1986). Master in Agrochemistry by Federal University of Viçosa (UFV, 1991). PhD in Chemistry by IQ / UNESP-Araraquara, SP (1999) (with an Internship at the CID / CSIC Department of Environmental Chemistry, Barcelona, Spain - 1997-1998). Post-Doctor in Biosensor area at University of Perpignan Via Domitia (UPVD, France, 2003-2004). Associate Professor

A B C


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(Analytical Chemistry area) in the Depto. of Chemistry of the UFV (1991-1994). Dr NUNES is currently a titular professor of the Department of Technological Chemistry of UFMA, where she has been developing research projects in the Environmental Chemistry and biosensors areas.

Magda Márcia BECKER Chemist at the Laboratory of

Pathology of the State of Roraima, Brazil

Chemistry teacher in High School of Roraima, Brazil

PhD in Biotechnology by Federal University of

Maranhão (UFMA, 2019) (with an internship at the University of Perpignan Via Domitia, France).

Has experience in chemistry, with emphasis on trace analysis, environmental chemistry, and amperometric biosensors.

E-mail: [email protected] Jean-Louis MARTY D.Sc., graduated in 1973 as Engineer of Biochemistry from National Institute of Applied Sciences in Lyon, received in 1980 Degree of Ph.D. in Agrochemical Sciences, and D.Sc. in 1987. Since 1993 he is a full Professor and began the research on biosensor at the University of Perpignan Via Domitia (France). He is involved in the development of biosensors in Environment, agro food and medical fields. He is a specialist of the development of AChE biosensors using recombinant enzymes. Many research works are devoted to enzyme sensors and immunosensors for the detection of marine and bacterial toxins, mycotoxins and various substrates in agro food industry. His expertise also covers the fictionalization of


88

surfaces for the immobilization of biomolecules. He initiated and maintains several collaborations with research groups all over the world. He has published more than 150 research articles. Gaëlle CATANANTE received her M.Sc. degree in chemistry from Aix-Marseille University, France in 2007 and received Ph.D. degree in biosensors from Universite de Perpignan, France in 2012. Her current fields of research interests are focusing on the conception of alternative analytic tools for assessment of different targets and development of biosensors in environmental agro food and medical fields.


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C17. Recovery of bioactive components from fruit processing wastes

Branka MOZETIC VODOPIVEC

University of Nova Gorica, School for viticulture and enology,

Wine research centre, Vipava, Slovenia

According to data of the UN Food and

Agriculture Organization, a third of all food produced

globally is lost or wasted. Most food waste occurs in the

supply chain, due to inadequate knowledge and

equipment, however fruit processing wastes disposal is

also growing problem. These wastes have high potential

for reuse as a concept of 'food for food', through the

production/extraction/acquisition of high‐value

compounds. Among these, phenols are the most

important from the antimicrobial and antioxidant aspect,

thus extending the possibility of their further use/re‐use

as food additives, nutraceuticals and also as agents

against plant and food pathogenic microorganisms.

The concentration of polyphenols is often greater

in the food processing wastes (pomaces) compared to

the final fruit product. Unfortunately, major parts of such

wastes still go to the landfill, and can thus cause

environmental pollution and huge losses of valuable

materials that could potentially be exploited for their

contents. Type and quantity of phenolic compounds in

wastes of fruit processing depends on chemical/physical

extraction techniques, fruit sort and cultivar and also

food processing technology which is more or less

efficient in transfer of phenols from original fruits to

product and wastes. Grape and apple pomace were


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already described as important source of polyphenols,

olive oil by-products can also represent precious

resource polyphenols, since 98% of olive fruit phenols

are lost during oil extraction into wastes.

Researchers are all the time trying to find

different ways of faster and most efficient and eco-

friendly extraction procedure for these compounds from

such plant material. The extraction techniques for

bioactive compounds from fruit wastes are mainly based

on solvent extraction (SE), while promising results are

also obtained from procedures like supercritical fluid

extraction (SFE), subcritical water extraction (SCW), use

of enzymes, hydrolysis, ultrasounds, microwaves and

recently also with ionic liquids.

Branka MOZETIČ VODOPIVEC

PhD in 2004 in Biotechnical Sciences – Food Science – University of Ljubljana, Biotechnical Faculty

Habilitation for Food Technology field At University

of Nova Gorica – since 2016 associate professor

Professor for Organic chemistry in viticulture and enology, Analytical methods in wine production, Controle of quality of grapes and wines, Enological Chemistry in School for viticulture and enology, University of Nova Gorica, Slovenia

Dean of School for viticulture and enology since 2011 – at University of Nova Gorica, Slovenia

Researcher of Wine research centre

University of Nova Gorica, School for viticulture and enology, Lanthieri Mansion, Glavni trg 8, Vipava,


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Slovenia

Email:[email protected], [email protected]

Phone: +386 59099701

Publications: http://izumbib.izum.si/bibliografije/Y20190510133242-A5062243.html

Research interests

Plant polyphenols (fruit, olives, grapes, juices, wine, etc.) isolation, quantification, identification using HPLC-DAD-MS/MS and UV-Vis spectrometry

Plant phenolics from fruit processing wastes – isolation, antioxidant and antimicrobial properties in real food environments

Piranoanthocyanins in wine – in relation to yeasts

Plant phenolics in relation to different environment impacts (maturation, storage, vintage, climatic impacts, processing technology ...)

Selected publication

Mozetič Vodopivec B., Trebše P., Hribar J.,

Determination and quantitation of anthocyanins and

hydroxycinnamic acids in different cultivars of sweet

cherries (Prunus avium L.) from Nova Gorica region

(Slovenia). Food technology and biotechnology,

ISSN 1330-9862, 2002, vol. 40, no. 3, str. 207-212.

Jerman T., Trebše P., Mozetič Vodopivec B., Ultrasound-assisted solid liquid extraction (USLE) of olive fruit (Olea euro- paea) phenolic compounds. Food chemistry, ISSN 0308-8146. [Print ed.], 2010, issue 1, vol. 123, str. 175-182, doi: 10.1016/j.foodchem.2010.04.006.

Jerman T., Mozetič Vodopivec B., Ultrasonic extraction of phenols from olive mill wastewater: comparison with conventional methods. Journal of


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agricultural and food chemistry, ISSN 0021-8561, 2011, vol. 59, no. 24, str. 12725-12731

Jerman T., Mozetič Vodopivec B. Optimisation of olive oil phenol extraction conditions using a high-power probe ultrasonication. Food chemistry, ISSN 0308-8146. [Print ed.], 2012, vol. 134, issue 4, str. 2481-2488

Jerman T., Mozetič Vodopivec B., The fate of olive fruit phenols during commercial olive oil processing : traditional press versus continuous two- and three-phase centrifuge. V: 1st Euro-Mediterranean Symposium 18-21 April 2011 Avignon, France. RENARD, Catherine (ur.). Fruit & Veg Processing, (Lebensmittel-Wissenschaft + Technologie, ISSN 0023-6438, vol. 49, no. 2). Amsterdam [etc.]: Elsevier, 2012, vol. 49, no. 2, str. 267-274,

Jerman T., Golc-Wondra A., Vrhovšek U., Mozetič Vodopivec B., Phenolic profiling of olives and their olive oil process-derived matrices using UPLC-DAD-ESI-QTOF-HRMS analysis. Journal of agricultural and food chemistry, ISSN 0021-8561, 2015, vol. 63, iss. 17, str. 3859-3872,

Jerman T., Golc-Wondra A., Vrhovšek U., Sivilotti P., Mozetič Vodopivec B., Olive fruit phenols transfer, transformation, and partition trail during laboratory-scale olive oil processing. Journal of agricultural and food chemistry, ISSN 0021-8561, 2015, vol. 63, no. 18, str. 4570-4579,

Bat K., Eler K., Mazej D., Mozetič Vodopivec B., Mulič I., Kump P., Ogrinc N., Isotopic and elemental characterisation of Slovenian apple juice according to geographical origin: preliminary results. Food chemistry, ISSN 0308-8146. [Print ed.], 2016, vol. 203, str. 86-94,

Klančnik A., Šikić Pogačar M., Troš, K., Tušek-Žnidarič M., Mozetič Vodopivec B., Smole Možina S.,. Anti-Campylobacter activity of resveratrol and an


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extract from waste Pinot noir grape skins and seeds, and resistance of C. jejuni planktonic and biofilm cells, mediated via the CmeABC efflux pump. Journal of applied microbiology, ISSN 1364-5072, Jan. 2017, vol. 122, iss. 1, str. 65-77,

Trošt K, Klančni A, Mozetič Vodopivec B., Sternad Lemut M., Jug Novšak K., Raspor P., Smole Možina S., Polyphenol, antioxidant and antimicrobial potential of six different white and red wine grape processing leftovers. Journal of the science of food and agriculture, ISSN 1097-0010, 2016, vol. 96, iss. 14, str. 4809-4820


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C18. The role of genetic tests in weight loss

Antoanela CURICI (1,2)

1-Faculty of Medicine, “Carol Davila” University in Medicine

and Pharmacy Bucharest

2-Synevo Romania

According to the Centre for Disease Control and

Prevention (CDC), in the past two decades, there has

been a steady rise in adult obesity in the United States,

from 19.4% in 1997 to 35.7% in 2010. Between 2011

and 2014, the prevalence of obesity was 36.5% in adults

and 17% in teenagers (1).

Also, a recent report by the World Health

Organization (WHO) shows that the entire Europe will

have to cope with the obesity epidemic, which will reach

alarming proportions in 2030. According to WHO

statistics, global prevalence of obesity has tripled since

1975. In 2016, the total number of overweight adults

exceeded 1.9 billion (including over 350 million obese

people), and about 10% of children were considered

overweight or obese. Obesity causes cardiovascular

diseases, type 2 diabetes or some forms of cancer

(approximately 25-30% of the cases of breast, colon,

endometrial, kidney and oesophageal cancer) (2).

In Romania, according to the PREDATORR

national epidemiologic study, initiated in 2013

(“Prevalence of diabetes mellitus, prediabetes,

overweight, obesity, dyslipidaemia, hyperuricaemia and

chronic kidney disease”), a global prevalence of obesity

(31.9%), overweight (34.7%), abdominal obesity


95

(73.9%) and metabolic syndrome (38.5%) was recorded

(3).

The pathogenesis of obesity is certainly complex,

involving multiple interactions between behavioural,

environmental and genetic factors. The increasing

prevalence of obesity can be partly attributed to

excessive food intake and relatively sedentary lifestyles

of modern times, but relatively recent studies have

shown that genetic factors significantly affect the risk of

becoming obese. Thus, it is estimated that ~ 30% - 40%

of the BMI (body mass index) variation can be attributed

to genetics and 60-70% to the environment (4).

There is a bidirectional relationship between

nutrition and the human genome, that has led to the

definition of new entities - nutrigenetics and

nutrigenomics. On the one hand, nutrigenetics studies

how the individual genetic profile can influence the

body’s response to various nutrients in the diet, and, on

the other hand, nutrigenomics analyses the effects of

bioactive components in food on gene expression (5).

Nutrigenetic tests aim at establishing the genetic

profile of a person in order to establish a personalised

diet that leads to weight loss and health improvement

(6).

Each person is the unique product of generations

of accumulation and combination of different genetic

traits. Some of those traits have negative effects on our

health. With the latest technology, it is now finally

possible to examine one's genes and determine his

personal health risks and strengths. In many cases,

taking advantage of this knowledge, and following some

precautionary measures, the diseases may be


96

prevented. This is the next step in preventive medicine

and a new generation of health care.

References

1. Ogden CL, Carroll MD, Fryar CD, Flegal KM. Prevalence of

Obesity Among Adults and Youth: United States 2011-2014.

NCHS Data Brief., No129, November 2015. Ref Type: Internet

Communication.

https://www.cdc.gov/nchs/data/databriefs/db219.pdf.

2. World Health Organization. Obesity and Overweight. Fact sheet

(reviewed February 2018). Ref Type: Internet Communication.

http://www.who.int/mediacentre/factsheets/fs311/en.

3. Popa S, Moţa M, Popa A, Moţa E, Serafinceanu C, Guja C,

Catrinoiu D, Hâncu N, Lichiardopol R, Bala C, Popa A, Roman

G, Radulian G, Timar R, Mihai B. Prevalence of

Overweight/Obesity, Abdominal Obesity and Metabolic

Syndrome and Atypical Cardiometabolic Phenotypes in the

Adult Romanian Population: PREDATORR Study. J Endocrinol

Invest. 2016 Sep;39(9):1045-53.

4. Pi-Sunyer FX. The Obesity Epidemic: Pathophysiology and

Consequences of Obesity. Obes Res. 2002 Dec;10 Suppl

2:97S.

5. Gregori D, Foltran F, Verduci E, Ballali S, Franchin L, Ghidina M,

Halpern GM, Giovannini M. A Genetic Perspective on Nutritional

profiles: Do We Still Need Them? J Nutrigenet Nutrigenomics.

2011;4(1):25-35.

6. Coletta A and Kreider RB. Genetic Profiling for Weight Loss:

Potential Candidate Genes. Bioenergetics 2015, 4:2 DOI:

10.4172/2167-7662.1000126


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Antoanela CURICI

Laboratory Medicine, MD

PhD (2015) in Medicine - Cellular biology and Histology, “Carol Davila” University of Medicine and Pharmacy Bucharest

Assistant Professor, Department of Cellular and

Molecular Biology and Histology, Faculty of Medicine, “Carol Davila” University of Medicine and Pharmacy Bucharest

Medical Director, Synevo Romania

Industriilor street, no. 25, Chiajna, Ilfov County


Phone: +40747.086.091

Research interests

Main research interests are clinical chemistry,

hematology, molecular biology and genetic disease -

correlations between the presence of different

polymorphism and clinical aspects.

Also improving quality in clinical laboratories, by

implementing and monitoring specific indicators.


Botezatu D, Tovaru M, Georgescu SR, Leahu OD,

Giurcaneanu C, Curici A. Interplay between prolactin

and pathogenesis of psoriasis vulgaris MAEDICA – a

Journal of Clinical Medicine 2016; 11(3):232-240.

Popescu LM, Curici A, Wang E, Zhang H, Hu S,

Gherghiceanu M. Telocytes and putative stem cells in

ageing human heart, J Cell Mol Med, 2015; 19(1):31-45.


98

Curici A, Codrici E, Mihai S, Pistol Tanase C, Popescu

BO, Ceafalan LC. CD105/endoglin expression in a

mouse model of acute muscle contusion, Romanian

Journal of neurology Volume XIII, No. 4, 2014, 186 -

192.

Cretoiu SM, Simionescu AA, Caravia L, Curici A,

Cretoiu D, Popescu LM. Complex effects of imatinib on

spontaneous and oxytocin-induced contractions in

human non-pregnant myometrium, Acta Physiol Hung,

2011; 98(3):329-38.

Popescu LM, Vidulescu C, Curici A, Caravia L,

Simionescu AA, Ciontea SM, Simion S. Imatinib inhibits

spontaneous rhythmic contractions of human uterus and

intestine, Eur J Pharmacol, 2006; 546(1-3):177-81.


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C19. Hygienic design for sustainability

Liviu GACEU

Faculty of Food and Tourism, Transilvania University of Brasov, Romania

This presentation starts with the definition of Hygienic design concept and European Hygienic Engineering & Design Group as a non-profit consortium of: Equipment manufacturers; Food industries; Suppliers to the food industries; Research institutes and universities; Public health authorities. Since 1989, the European Hygienic Engineering & Design Group is guiding the food industry in hygienic design solutions by offering practical guidelines, test procedures, training and education.

The Benefits of Hygienic Design are: Hazard control (allergens, microbiological, chemical, foreign matter); Improved product quality; Life cycle cost reduction; Quick change over execution; Pest control; Human safety; Housekeeping; Equipment reliability; Dietary compliance (Halal, Kosher, organic); Regulatory Compliance.

The objectives of EHEDG are :guidance on hygienic design and engineering to ensure food safety and quality: to offer a platform for the food industry to discuss issues on hygienic design; to develop guideline documents on hygienic design requirements and practices based on science and technology to ensure compliance to legislation; to maintain a transparent and unambiguous hygienic equipment certification scheme: to identify areas were hygienic design knowledge is insufficient and initiate and promote research and development in those areas.

The activity of EHEDG is marked by: guidelines, like: Air Handling; Bakery Equipment; Cleaning in Place;


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Cleaning Validation; Cleaning & Disinfection; Conveyor Systems; Dry Materials Handling; Fish Processing; Food Refrigeration; Heat Treatment; Hygienic Systems Integration; Hygienic Design Principles; Materials of Construction in Contact with Food .

The second subject evaluated in this presentation is: “Environmental impact during sanitation of Hygienic designed equipment vs conventionally designed equipment”.

Cleaning and Disinfection (Sanitation) of equipment and facilities in food industry is critical for food safety and quality. Frequency of sanitation is generally high to reach the high hygiene standards required for food safety reasons. Sanitation is one of the most water and energy consuming operations and wastewater generator in the food industries. As indicated in the FDM BREF 2006, cleaning and disinfection is the main water consumer operation in most food sectors, such as dairy, fish, drink or meat, with the amount depending on the type and size of equipment to be cleaned and the materials processed. The question that is following naturally is: “Hygienic Design is assuring Food Safety but is also environmental friendly?” The answer is done by the presentation of ECODHYBAT project that applied the big concept of hygienic design.

The project approached activities at industrial scale which are representative of dairy and fish processing. The equipment configuration was re-designed following hygienic criteria under an integrated approach. Sanitation demonstrative trials were performed in order to evaluate the environmental improvement achieved while hygienic level in equipment is maintained.

The expected results have to demonstrate that the design of productive equipment following hygienic


101

criteria reduces the overall impact of the sanitation operations while maintaining hygienic assurance levels.

The hygienic diagnosis was carried out in two fish processing lines in PESCANOVA. In the first case, the following equipment was selected: Mixer batter tank; Mixer paste tank; Mixer bread tank; Lobular pump; Butterfly valve; Ball valve; Hose coupling. In the second case study was used a production line of packaged milk. The following equipment was selected: Flash cooler; Aseptic cross-way valves; Tank cleaning devices; Dumping devices UHT; Agitators seal; Heat keeping tubes; Tank cover in aseptic tanks; End of the product pipe in aseptic lines; Anti splashing device for discharge tubes; Recover product machines (hopper and piston system); Conveyor belts; Centrifugal pumps; Single seat valves; Flow meters; Sensors.

The final results show reducing of water consumption by 36,5% and total water use (including indirect water use) by 30,5%. Direct energy consumption was rescuer by 27% and total energy consumption (including indirect energy consumption) was reduced by 33%. GHG emissions of CO2 was reduced by 34,7% and the volume of waste water was reduced by 36,5%.

Liviu GACEU

PhD (2001) Mechanical

Engineering – Transilvania

University of Brasov;

Habilitation in Engineering and

Management, Transilvania

University of Brasov;

Coordinator of Food Product Engineering study

programme (4 years), Transilvania University of

Brasov;


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President of Romanian Society for Agriculture,

Foood, Environment and Tourism, www.rosita.ro

President of the Romanian European Hygienic

Engineering and Design Group Regional Section:

https://ehedg.ro/

Ambassador for Romania of Global Harmonization

Initiative:

https://www.globalharmonization.net/romania

Chief Editor of the Journal of EcoAgroturism,

http://www.unitbv.ro/ecoagrituourism

Teacher of the course-level degree in following

subjects:

a. Computer Aided Design b. Machinery and equipment for milling and baking c. Application of the computer in Food and Tourism

Research team member of the Interdisciplinary Platform RENATSIL, Transilvania University of Brasov.

Computer Aided Design and Modeling: AUTOCAD, PRO-ENGINEER, I-DEAS, ABAQUS, ANSYS

Knowledge in computer networks, and operating the following programs: Microsoft Office, FoxPro, Adobe Photoshop, CorelDraw, MathLab, Labview

Castelului street no.148, Brasov, Romania

Phone:0268472222

Research Interests

Automation in food and agriculture

Technologies, equipment and facilities for milling and

baking

Grain-drying equipment

Programmer-computing;

Applied Electronics;


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Computer hardware and software;


Buculei A., Amariei S., Oroian M., Gutt G., Gaceu L.,

Birca A., Metals migration between product and metallic

package in canned meat, LWT - Food Science and

Technology, Volume 58, Issue 2, October 2014, Pages

364-374, Elsevier, ISSN: 0023-6438;

Sukhmanov V., Shatalov V., Petrova J., Birca A., Gaceu

L., The influence of high pressure on bio-system reaction

kinetics and the preservation of vitamin C, LWT - Food

Science and Technology, Volume 58, Issue 2, October

2014, Pages 375-380, Elsevier, ISSN: 0023-6438;

Thierheimer W., Tane N., Gruia R., Gaceu L.,

Thierheimer D., Ola D., Cojocaru V., Clinciu M., Risk

Arising from Transport Activities, Environmental

Engineering and Management Journal, December 2010,

pag. 1167-1170. ISSN: 1582-9596. Gheorghe Asachi

Technical University of Iasi, Romania;

Oprea O. B., Gaceu L., Tucu D., Valorisation of winery

waste by using GSP (grape seed powder) as flour

substitution in bakery industry, Symposium "Actual Tasks

on Agricultural Engineering", Opatija, Croatia, 2017, pp.

371-376, ISSN 1848-4425.

Frioui M., Shamtsyan M., Gaceu L., Oprea O. B., Mnerie

D., Rheological influence of (1–3)(1–6) mushrooms ß-

glucan, used as flour substitution in bakery industry,

Symposium "Actual Tasks on Agricultural Engineering",

Opatija, Croatia, 2017, pp. 377-384 , ISSN 1848-4425;

Spirchez C, Lepadatescu B., Gaceu L., Comparative

study of different effectiveness of building insulation of

wood in Romania, 12 th International Conference-

Standardization,Prototypes and Quality A means of


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Balkan Countries Collaboration, pag.135-138, 22-24,

2015, Kocaeli Univ. Izmit, Turkey;

Oprea O.B., Apostol L., Bungau S., Cioca G., Samuel

A.D., Badea M., Gaceu L., Researches on the Chemical

Composition and the Rheological Properties of Wheat and

Grape Epicarp Flour Mixes, REV.CHIM.(Bucharest),

Vol.69, Nr. 1, 2018, pag.70-75, ISSN 2537-5733.


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C20. Potential anticancer effects of food-related components

Cristina POPOVICI

Faculty of Food Technology, Technical University of

Moldova

Cancer imposes an enormous burden on societies in more and less economically developed countries alike. The occurrence of cancer is increasing due to the growth and ageing of the population, as well as the increasing prevalence of established risk factors, such as smoking, being overweight (relating to abnormal and/or inappropriate food consumption), physical inactivity, and changing reproductive patterns associated with urbanization and economic development.

There are three crucial characteristics of innovative anticancer agents relate to their capability in killing cancer cells resistant to pro-apoptotic stimuli (such as metastatic cancer cells), killing cancer cells through non-apoptotic-mediated cell death pathway, and actually impairing the biological behaviour of CSCs.

The majority of food-related components that are beneficial to human health are of natural origin; some of them display anticancer effects. However, the diet profiles of people from Africa, North America, South America, Asia, Australia, and Eastern, Western, North and South Europe markedly differ.

There are thousands food-related components of natural origin that can more or less impair cancer progression, and these components can originate from plants, macroscopic mushrooms, insects, and terrestrial (other than insects) and marine invertebrates. From more than a gross picture, diets based on a high consumption of “plants” (vegetables and fruits), fish and


106

insects and a low consumption of alcohol and mammalian fat apparently lead to a lower cancer incidence than diets based on a weak consumption of plants and a high consumption of red meal, mammalian fat and alcohol.

Potential anticancer effects of food-related components should be further researched in clinical trials on different models for their effectiveness and toxicological documentation. Furthermore, extensive research work should be carried out on these components to evaluate their possible applications, toxicological and particular genotoxic profile against a wide range of cancer in both either in-vitro or in-vivo.

Cristina POPOVICI PhD (2009) in Engineering

(Food Technology), Technical University of Moldova

Associate Professor (2017) of Food Science and Technology at Department of Food and Nutrition, Faculty of Food Technology, Technical University of Moldova

Habilitation (2019) in Engineering (Food Technology), Technical University of Moldova

Coordinator (2017) of International Projects at Technical University of Moldova

168 Stefan cel Mare bd., MD 2004 Chisinau, Republic of Moldova


Phone: (+373) 68241547

Researcher ID: C-3919-2019,

ORCID ID: 0000-0002-6985-8158

Scopus Author ID: 57200654499


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Research interests Main research interests are focused on following

topics: anticancer properties of food-related components; antioxidant profile and quality characteristics evaluation; advanced extraction methods (supercritical CO2, solid/liquid) of bioactive compounds; food fortification (in vitro and in vivo studies), nut milk processing and characterization; vegetable oil processing and stabilization; fermented goat milk products with functional properties. She has experience in teaching food science and technology courses and supervising bachelor, master and doctoral thesis.

Actually she is coordinating independent project for young researchers and is a team member of 8 national and international projects (INTERTOOL, ELEVATE, NUTRILAB).

She is also a member of the Scientific Seminar for the evaluation of PhD thesis at the Technical University of Moldova, she is an active member of the Scientific Committees of International Conferences (Bulgaria, Romania, Ukraine) and actually is a member of the editorial board of the Ukrainian Food Journal, ISSN 2304-974X. During time, she published more than 80 papers in different conference proceedings, specialized journals and 6 teaching materials. Selected publications Popovici C, Migalatiev O, Gaceu L, Golubi R, Caragia V.

Supercritical carbon dioxide extraction of lycopene from

industrial tomato waste. Modern Technologies in the Food

Industry 2018; 43-50.

Saykova I, Tylkowsky B, Popovici C. Extraction of

phenolic and flavonoid compounds from solid wastes of

grape seed oil production by cold pressing. J Chem Tech

and Metallurgy 2018; 2 (53): 177-190.


108

Popovici C, Caragia V, Cartasev A, Migaltiev O, Coev

Gh., Golubi R., Bogdan N., Grumeza I. InoBioProd:

Innovation Challenges and Scientific Perspectives.

Ukrainian Food Journal 2017; 6(2): 379-390.

Baerle A, Popovici C, Radu O, Tatarov P. Effect of

synthetic antioxidants on the oxidative stability of cold

pressed walnut oil. Journal of Food and Packaging

Science, Technique and Technologies 2016; Year V, No

9, 19-24.

Popovici C, Mija N, Birca A, Iatco I. Compliance with

labeling legislation of the Republic of Moldova in the field

of confectionery products. Acta Universitatis Cibiniensis.

Series E: Food Technology 2013; 17 (2): 61–67.

Popovici C, Saykova I, Tylkowsky B. Evaluation de

l’activité antioxydant des composés phénoliques par la

réactivité avec le radical libre DPPH. Revue électronique

internationale pour la science et la technologie 2009; 4 :

26 – 39.

Popovici C. The influence of natural antioxidants on the

oxidative stability of iodine – fortified sunflower oil in the

process of storage. Surface Engineering and Applied

Electrochemistry 2008; 44 (5): 415-421.

Sturza R, Deseatnicova O, Popovici C, Gudumac V,

Nastas I. Influence of iodinated oil and margarine on the

thyroid system of rats. Chemistry Journal of Moldova.

General, industrial and ecological chemistry 2008; 3 (1):

77 – 84.


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C21. Nutrition in childhood - the premise for a healthy adulthood

Bianca POPOVICI


Romania

Growing and developing processes of human are based on genetic factors as well as environmental factors which include also alimentation. The very first years of life when these processes are very intense and rapid are crucial for the future adulthood period. Each stage of development in childhood has a specific type of alimentation adapted to the specific condition, to the metabolic requirements and the capacity of the child correlated with gestational age, weight, age and particular condition.

We already know that features like weight, blood pressure the blood level of glucose or uric acid but also chronic diseases such obesity, hypertension, cardiovascular disease, diabetes mellitus type 2 have a inherited pattern and follow a “tracking phenomenon” from early childhood to the adulthood life. More, these chronic degenerative pathology are originated in fetal life and any favourable environmental condition such inappropriate alimentation can determinate clinical expression of it.

Several aspects regarding the pattern alimentation in children will be presented and also the connection with chronic diseases such obesity, high blood pressure, and metabolic syndrome. It shall be discussed the possible influence of the consumption of non-nutritious food and beverages on the health status and how these can influence the course of degenerative cardiovascular diseases starting from childhood.


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Finnaly, some new perspectives of healthy alimentation will be suggested in attempted of improving the knowledge and education of the people.

Bianca Elena POPOVICI

PhD (2010) in Pediatrics, “Gr.

T. Popa” University of Medicine

an Farmacy Iasi

Associate Professor,

Department of Pediatrics,

Faculty of Medicine,

“Transilvania” University Brasov

B-dul Eroilor nr. 29 Brașov România


Phone: (+40) 773724009

Research interests

Main research interests are cardiovascular

diseases in children, hypertension, obesity, metabolic

syndrome and the alimentation disorders connected.


Popovici BE. Epidemiology, clinic and diagnosis of

essential hypertension in childhood. Printing House StudIs,

Iași, 2015. ISBN 978-606-775-044-7

Buzinschi S., Dracea L, Epure H, Falup-Pecurariu O,

Popovici BE, Vodă D. Nutrition in pediatrics. Lux Libris

Printing House, Brașov, 2006. ISBN(10)-973-9458-72-6;

Popovici B.E., Mitrica M., Cosor A., The influention of the

alimentation pattern in adolescents health in Brasov

region. New Trends on Sensing- Monitoring- Telediagnosis

for Life Sciences, Brasov, Romania - July 24-26, 2014


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Popovici B.E., Mitrica M., Moga M.. Cardiovascular risk

factors impact in childhood. 54th Annual Meeting

European Society for Paediatric Research October 10th-

14th, 2013 –Porto, Portugal, Book of Abstract; 374

Popovici BE, Brumaru O. Aspects regarding of

cardiovascular risk factors in children. Romanian Journal

of Pediatrics 2010, vol LIX, no 4: 322-328

Popovici BE, Brumaru O. Correlations between essential

hypertension and carotid intima-media thickness in

children. Medical-Surgical Journal of The Medical and

Scientists Society Iaşi 2011 Ian-Martie 115(1)


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C22. Accidental poisoning in children and chemical safety

Elena Madalina PETRAN

Faculty of Medicine, University of Medicine and Pharmacy

Carol Davila, Bucharest, Romania

Accidental poisoning is a frequent incident in children life events. In Europe, almost 20% of all presentations and admissions in a children hospital are the result of accidental poisoning. This type of pathology is most common in young children, age group 1-5 years old, especially boys, when they start to explore and be aware of things around them.

Most poisonings occur at home, where different substances are not properly stored and misplaced giving the children access to them. A lot of household items ca be poisonous, like cleaning products, insecticides, different drugs, abuse substances like alcohol and cigarettes, decorative plants, petroleum by-products and other substances like carbon monoxide and nitrates. In many cases parents have witnessed the exposure to poisons and can describe and provide useful data about recipients, chemical composition, route of poisoning, quantity, time elapsed from occurrence, sings and symptoms that appear after the incident and first aid manoeuvres applied. In other cases, medical specialists must rapidly recognise, diagnose, investigate and start specific treatment to prevent severe outcomes. Specific treatment can be recommended and started by specialised medical staff according to poison way of action. Some poisonings require only monitoring, others gastric lavage, administration of activated charcoal, blood tests, specific medical investigations


113

and antidote treatment. According to poison’s chemical properties, time elapsed from the incident till specific treatment is started and the existence of comorbidities, the outcome of poisoned children can vary from full recovery to death.

Children are vulnerable to different threats in their environment as they are not yet aware of various dangers, as substances with toxic potential. Adult supervision and caution in placing toxicants out of children reach is critical and an important problem of public health and safety. Key words: accidental poisoning, children, chemical safety

Elena Madalina PETRAN PhD (2012) in Medicine (Clinical

Toxicology), “Carol Davila University of

Medicine and Pharmacy, Bucharest, Romania

2006-present: Pediatrics MD, “Grigore Alexandrescu

Emergency Clinical Hospital Bucharest, Romania

2015-present: Assistant lecturer, “Carol Davila University of Medicine and Pharmacy Bucharest, Romania, General Medicine Faculty, Biochemistry Department


Research interests

Understanding how biochemical mechanisms work in normal and ill children is main goal in managing normal development, using alternative therapies and also studying toxicological and metabolic implications in drugs and food safety.


114

Selected publications Dascultu D; Petran M E., Epidemiology of acute

metformin poisoning in children: a 5 year study, Clinical Toxicology Volume: 56 Issue: 6 Pages: 519-520 Meeting Abstract: 146 Published: 2018

Iosif L.; Gaman L. E.; Gilca, M.; Petran M. et al., Flavonoids are preserving phenolic acids in vegetable oils under microwave heating, Proceedings Of The Nutrition Society Volume: 75 Issue: OCE 2 Pages: E56-E56 Published: JAN 2016

Petran E. M; Stanca S; Ulmeanu C, Severe acute poisoning in children: A 5-year retrospective study, E.Clinical Toxicology Volume: 53 Issue: 4 Pages: 293-293 Meeting Abstract: 126 Published: MAY 2015


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C23. Cortisol, the alcohol of all our days

Laura GAMAN, Irina STOIAN, Marilena GILCA, Valeriu ATANASIU

Faculty of Medicine, University of Medicine and Pharmacy

Carol Davila, Bucharest, Romania

It is well-known that daily stress it is a risk factor for a variety of mental and physical health problems, like heart disease, asthma, neurodegenerative disorders, anxiety disorders, depression, chronic pain, , autoimmune diseases, fetal growth restriction, etc.

Many researche papers shows that chronic stress-induced cortisol elevation will affect feedback regulation leading to longterm damages in physical and psychological health

In this cotext we try to analyze how deep could be correlate the level of cortisol with this health problems and to purpose methods to reduce this.

Elena Laura GAMAN PhD (2006) in Pharmacy, „Carol

Davila” University of Medicine and Pharmacy Medicine

Associate Professor, Department of Biochemistry, Faculty of Medicine, „Carol Davila” University of Medicine and Pharmacy Medicine

Bdul Eroilor Sanitari no.8, sector5, Bucharest, Romania


Phone: (+40) 720049267


116

Research Interests Main research interest is the oxidative stress

associated with different diseases: mitochondrial disease in children, atherosclerosis and cardiovascular disease, neurological disease like schizophrenia, Alzheimer’s, diabetes, renal chronic disease. Selected Publications Gaman L, Dragos D, Vlad A, Robu G C, Radoi M P,

Stroica L, Badea M, Gilca M., Phytoceuticals in acute pancreatitis: targeting the balance between apoptosis and necrosis, Evidence-Based Complementary and Alternative Medicine, 2018, Article ID 5264592, 27 pages

Iosif L, Gaman L, Gilca M, Radoi M, Kovacs E, Stoian I, Dragos D., Vegetable oils microwave heating – CUPRAC, TEAC AND FRAP values in relation with oxidative parameters Rev de chimie 68 (8), 2017

Iosif L, Gaman L, Crihana I, Kovacs E, Stoian I, Lupescu O Microwave and electrical oven heating are having different effects on antioxidant/oxidative stress parameters of vegetable oils, Rev de chimie 67 (12), 2638-2642

Gilca M, Lixandru D, Gaman L, Vîrgolici B, Atanasiu V, Stoian I. Erythrocyte membrane stability to hydrogen peroxide is decreased in Alzheimer’s disease. Alzheimer Diseases & Associated Disorders, Oct-Dec; 28(4):358-63, 2014

Gilca M, Piriu G, Gaman L, Delia C, Iosif L, Atanasiu V, Stoian I A study of antioxidant activity in patients with schizophrenia taking atypical antipsychotics. Psychopharmacology, 2014., 231:4703-4710, 2014

Gilca M, Gaman L, Panait E, Stoian I, Atanasiu V. Chelidonium majus- and integrative review. Traditional information versus scientific findings, Forsch Komplementmed, 17(5):241-248, 2011


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C24. The role of exposome in allergic diseases and asthma

Ioana AGACHE


Romania

Ioana Octavia AGACHE

Medical degree “Carol Davila” University, Bucharest and absolved Magna Cum Laude her PhD in Internal Medicine.

Professor of Allergy and Clinical Immunology at Faculty of Medicine, Transilvania University of Brasov, Romania

President of the European Academy of Allergy and Clinical Immunology (EAACI) 2017-2019.

Research interests

Her research in the field of Asthma and Allergy and Clinical Immunology focused on asthma phenotypes and endotypes, immune modulation and immune tolerance and integrated management of allergic diseases, with a special focus on primary care and community pharmacists.

Ioana Agache is member of the Steering Committee and co-author of several international and European Guidelines, such as ARIA (Allergic Rhinitis and its impact on Asthma), EAACI Allergen Immunotherapy Guidelines and EAACI Food Allergy and Anaphylaxis Guidelines. Ioana Agache is actually Chair of the EAACI Guidelines on the use of Biologics in asthma and allergic diseases.

She is Editor of the Global Atlas of Asthma (2013), Global Atlas of Allergy (2014), Global Atlas of


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Allergic Rhinitis and Chronic Rhinosinusitis (2015), Implementing Precision Medicine in Best Practices of Chronic Airway Diseases (2018), Associate Editor of Clinical and Translational Allergy and a member of the Editorial Board for Allergy and Journal of Allergy and Clinical Immunology.

book of abstractsfshl.ro/2019/fshl-2019-book-of-abstracts.pdf · doina paula balaban, ovidius...

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