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Book of Abstracts
The 3rd International Conference on Bioscience
and Biotechnology
(BioTech 2018)
08th-09th March 2018
Colombo, Sri Lanka
Committee of the BioTech 2018
The International Institute of Knowledge Management (TIIKM)
Tel: +94(0) 11 3132827
ii
Disclaimer
The responsibility for opinions expressed, in articles, studies and other contributions in this
publication rests solely with their authors, and this publication does not constitute an
endorsement by the BioTech or TIIKM of the opinions so expressed in them.
Official website of the conference
www.bioscienceconference.com
Book of Abstracts of the 3rd International Conference on Bioscience and Biotechnology
(BioTech 2018)
Edited by Prof. Mark Smales and Dr. Chamari Hettiarachchi
ISBN 978-955-4903-95-1
Copyright @ 2018 TIIKM Publishing LLC
All rights are reserved according to the code of intellectual property act of Sri Lanka,
2003
Published by The International Institute of Knowledge Management (TIIKM), No:
531/18, Kotte Road, Pitakotte ,10100, Sri Lanka
Tel: +94(0) 11 3098521
Fax: +94(0) 11 2873371
iii
Hosting Partner:
University of Kent, United Kingdom
Academic Partners:
British College of Applied Studies (BCAS) Campus, Sri Lanka
Faculty of Food Technology and Biotechnology, University of Zagreb, Republic of Croatia
Anna University, Chennai, India
University of Malaya, Malaysia
Organized By:
The International Institute of Knowledge Management (TIIKM), Sri Lanka
PROF. MARK SMALES (Conference Co- Chair, BioTech 2018)
Professor of Industrial Biotechnology, Director of the
Industrial Biotechnology, University of Kent, United
Kingdom
DR. CHAMARI HETTIARACHCHI (Conference Co-Chair, BioTech 2018)
Senior lecturer in Molecular Biology and Biochemistry,
Department of Chemistry, University of Colombo
PROF. ATTA-UR-RAHMAN (Keynote Speaker, BioTech 2018)
Professor Emeritus/Patron-in-Chief, International Center
for Chemical and Biological Sciences, (H. E. J. Research
Institute of Chemistry and Dr. Panjwani Center for
Molecular Medicine and Drug Research) University of
Karachi, Karachi, Pakistan
PROF. DR. VLADIMIR MRŠA (Keynote Speaker, BioTech 2018)
Professor of Industrial Biotechnology, University of
Zagreb, Croatia
DR. ANURADHA DHANASEKARAN (Plenary Speaker, BioTech 2018)
Head of the Department of Biotechnology, Anna
University, India
DR. D. N.VINOD KUMAR (Session Chair, BioTech 2018)
National Institute of Oceanography, India
BioTech 2018 Committee
iv
DR. SELVAKUMAR DHARMARAJ (Session Chair, BioTech 2018)
Academy of Higher Education, India
DR. ANBU ARUNKUMAR (Session Chair, BioTech 2018)
Karpagam Academy of Higher Education, India
DR. PAVAN JUTUR (Session Chair, BioTech 2018)
International Centre for Genetic Engineering and
Biotechnology, India
DR. KASHIF AHMED (Session Chair, BioTech 2018)
NED University of Engineering & Technology, Pakistan
MR. ISANKA. P. GAMAGE (Conference Convener, BioTech 2018)
The International Institute of Knowledge
Management, Sri Lanka
MR. OSHADEE WITHANAWASAM (Conference Publication Chair, BioTech 2018)
The International Institute of Knowledge
Management, Sri Lanka
MS. ASHA RATHNAYAKE (Conference Secretariat, BioTech 2018)
The International Institute of Knowledge
Management, Sri Lanka
Editorial Board-ICOM 2013
Editors in Chief
Prof. Mark Smales, Professor of Industrial Biotechnology, Director of the Industrial Biotechnology, University
of Kent, United Kingdom
Dr. Chamari Hettiarachchi, Senior Lecturer in Molecular Biology and Biochemistry, Department of Chemistry,
University of Colombo
The Editorial Board is not responsible for the content of any research paper
Editorial Board - BioTech 2018
v
Prof. Oyaziwo Aluede, Department Of Educational Foundations And Management, Ambrose Alli
Dr. Amy Then Yee Hui, University of Malaya, Malaysia
Dr. Anupama Halmillawewa, University of Kelaniya, Sri Lanka
Prof. D. N. Rao, IIMS, India
Dr. Anushka Elvitigala, University of Sri Jayewardenepura, Sri Lanka
Dr. Farshid Talat, West Azarbaijan Agricultural and Natural Resources Research and Education Center, Iran
Dr. M. M. Kabir, East West University, Bangladesh
Prof. Deepal Mathew, University of Colombo, Sri Lanka
Asst. Prof. Azam Noori, Merrimack College, United States
Asst. Prof. Kashmir Singh, Panjab University, India
Dr. Sharmila Jayasena, University of Colombo, Sri Lanka
Dr. Murni Halim, Universiti Putra Malaysia, Malaysia
Dr. Siti Aqlima Ahmad, Universiti Putra Malaysia, Malaysia
Dr. Ahmad Muhaimin Roslan, Universiti Putra Malaysia, Malaysia
Dr. Mohamad Zulfazli Mohd Sobri, Universiti Putra Malaysia, Malaysia
Dr.C.D. Anuradha, Anna University, India
Dr. Mohamad Zulfazli Mohd Sobri, Universiti Putra Malaysia, Malaysia
Dr. Acga Cheng, University of Malaya, Malaysia
Scientific Committee - BioTech 2018
vi
MESSAGE FROM THE HOSTING PARTNER BioTech 2018
Dear Participants,
The University of Kent is delighted to welcome you to the 3rd International Conference on
Bioscience and Biotechnology 2018, Colombo, Sri Lanka, as a hosting partner of the event.
This meeting offers an exciting opportunity for the University of Kent to help foster and
develop new interactions between research scientists to share knowledge and instigate new
collaborations. The development of biotechnology, such that precision genome editing can be
undertaken and large scale sequencing is becoming a ‘routine’ tool of the trade, has given
scientists an unparalleled ability to manipulate and harness the power of biosciences and
apply this to health, agriculture and environmental issues (to name but a few) whilst ensuring
such tools are used in a responsible manner. The meeting here brings together delegates from
a wide range of disciplines to discuss their latest findings and interpretations across the
bioscience and biotechnology remit, providing a forum for the exchange of knowledge and
forging of new ideas.
We welcome you again to the conference and wish you a successful and productive meeting
in Colombo.
vii
MESSAGE FROM THE CONFERENCE CO-CHAIR BioTech 2018
Dear Participants,
It is with great pleasure that the I welcome you on behalf of the organizing committee to the
the 3rd International Conference on Bioscience and Biotechnology 2018 held in the beautiful
city of Colombo, Sri Lanka. This now established conference consists of 2 conference tracks,
Application of Biochemistry and Molecular Biology to Biotechnology and General
Biological Engineering. Within these two tracks there is a range of sessions and topics from
biodrug discovery and manufacturing to bioremediation and from biological systems and
models to nano-medicine. The full details of the wide and cross-disciplinary range of topics is
detailed on the website and abstract handbook. The breath of topics covered reflects the fact
that bioscience and biotechnology plays an important role in many facets of everyday life and
offers the potential to solve local and global challenges that face us all.
The scientific sessions cover cutting edge new science in the areas outlined. The extensive
program has been designed by the committee to give as many early career scientists the
opportunity to presentation alongside the high profile and keynote speakers that we have
attracted to the meeting. We thank all the speakers for agreeing to participate and those who
submitted abstracts. The poster session also provides opportunities for authors to present their
work and we encourage everyone to visit the posters and discuss the work with the authors.
The organizing committee would especially like to thank our sponsors. Their generosity helps
support this conference. I would also like to thank the rest of the organizing committee for
undertaking all their assigned tasks in a timely manner and working together so well.
Finally, the location, the scientific program, and the social events have been designed to
encourage networking and enhance scientific discussion amongst participants. It is
anticipated that the oral and poster presentations in this inspiring environment will generate
new ideas and the initiation of fruitful novel research projects and collaborations. Participants
and their engagement make a scientific meeting and it is up to us all to ensure this is a
successful conference that we all reflect upon favorably. We thank you for participating in
the conference and wish you a successful and productive meeting in Colombo.
Mark Smales
Conference Co-Chair BioTech 2018
Professor of Industrial Biotechnology,
Director of the Industrial Biotechnology,
University of Kent,
United Kingdom.
viii
MESSAGE FROM THE CONFERENCE CO-CHAIR BioTech 2018
On behalf of the organizing committee, it is my honor and pleasure to welcome all research
scientists, academics, postgraduate and graduate students from all over the world to
Colombo, Sri Lanka for the 3rd International Conference on Bioscience and Biotechnology
2018. The conference will be held from 8th to 9th March under the theme “Pursuing
innovation in Bioscience and Biotechnology” .
The word "biotechnology" has received its importance and significance in last two decades,
which is just unprecedented. Probability and possibilities behind this kind of attention
towards biotechnology is due to its unlimited potential to serve and benefit humanity. It has a
significant impact in almost all the domains of human life, may it be health, environment,
food and agriculture.
Bioscience and Biotechnology 2018 depends on generous knowledge donations of experts to
promote the scientific discoveries. It will be committed to promote open, transparent, and
international scientific exchange on the “New research and innovations in Bioscience and
Biotechnology” and contribute to the improvement of efforts taken to tackle local and global
impacts of Biological Science.
The conference is organized as a set of main tracks; Application of Biochemistry and
Molecular Biology to Biotechnology, General Biological Engineering, Biomedical
Engineering and Bioinformatics, Computational Biology for Biotechnology and other related
tracks Biostatics, Biometric, Bio measurement, Biomechanics, Biophysics, and System
Biology. It focuses on importance of Bioscience and Biotechnology through the keynotes
speeches, Plenary speeches, invited talks, technical programs and workshops.
I hope this two days of scientific discussions and presentations by leading experts in the
World as well as new research carried out by young scientist will provide a perfect amalgam
for novel discovery in both basic and applied research in the Biotechnology. The outcome of
the conference will pave ways to many new developments in the field of Biotechnology
Worldwide. I am sure that you all will tremendously benefit from the deliberation of this
conference in a warm and friendly environment. Your presence and deliberation will make
this conference remarkably successful in all aspects of Biotechnology.
I congratulate all of you for your commitment and active participation and wish you all the
success.
Dr. Chamari Hettiarachchi
Conference Co –Chair BioTech 2018
Senior Lecturer in Molecular Biology and Biochemistry,
Department of Chemistry,
University of Colombo,
Sri Lanka.
ix
Table of Contents Page No
New Challenges for Mammalian Expression Systems and Cell
Engineering Strategies Applied to Overcome These
J. Budge
02
Molecular Mechanisms of Yeast Cell Wall Biosynthesis and How
Can We Use it to Engineer the Surface of the Cell
V. Mrša, A. Sk. Hossain, A. Grbavac, M. Lozančić M and R. Teparić
03
Arabidopsis to Rice: Applying Knowledge Gained from Model Plant
to Enhance Agriculturally Important Crop Plants
C. Hettiarachchi
04
A1 01 Development of E. coli Platforms for Diterpene Synthesis
M. Fuchs
06
A2
02 Functional and Structual Characterization of Setaria digitata Novel
Protein (SdNP)
M. Gowthami, K.Varmila, Gayathri. N. Silva and R.S. Dassanayake
07
A3 03 Analysis of Papillary Thyroid Carcinoma Associated Biomarkers and
Genetic Abnormalities to Determine the Risk of Developing Cancer
in Patients Diagnosed with Different Thyroid Disorders
P. Shirani, T. Dhanushika, F.I. Moulana, A.A.H. Priyani, M.C.V. de
Silva and R.S. Dassanayake
08
ORAL PRESENTATIONS
APPLICATION OF BIOCHEMISTRY AND MOLECULAR BIOLOGY TO
BIOTECHNOLOGY (A)
KEYNOTE ABSTRACTS
x
A4 04 Behavioral and Biochemical Effects of Banana (Musa sapientum L)
Peel Extract Following Noise Stress in Male Mice
N. Samad and A. Muneer
09
A5 05 Phytoremediation Potential of Legume Plant Cajanus cajan (Pigeon
Pea) on Petroleum Oily Sludge Contaminated Soil
I. A. Allamin, S.A. Ahmad, N.A. Yasid, S.R.S. Abdullah, M.I.E. Halmi
and M.Y. Shukor
10
A6 06 Response Surface-Based Optimization and Kinetic Studies of Phenol
Degradation by Alcaligenes sp. AQ5-02
A. Aisami, N.A. Yasid, W.L.W. Johari, S.A. Ahmad and M.Y. Shukor
11
A7 07 Isolation and Identification of Cadmium Sensing Regulator – cadC
from Bacillus megaterium strain TWSL-4
W.M.N.H. Kumari, C.D. Wijayarathna and N.V. Chandrasekharan
12
A8 08 Efficient Heterotrophic Mangrove Bacteria for Carbon Sequestration
through Microbially Induced Calcium Carbonate Precipitation
(MICP)
N.V. Kumar, P. Ammini and J. Vijayan
13
A9 09 siRNA Mediated RNA Interference of Novel Parasitic Nematode-
Specific Protein of Setaria digitata in Culex quinquefasciatus
Showed Molting and Movement Detects and Development Arrest
M.B.C.L. Somarathne
14
A10 10 High Throughput Screening of Bioactive Secondary Metabolites in
Sri Lankan Medicinal Plants by Microfractionation and Targeted
Isolation of Antibacterial Flavonoids
S. Rajendran, S. Mohotti, T. Muhammad, A.A. Strömstedt, A.
Adhikari, E.D. de Silva, U. Göransson, C.M. Hettiarachchi and S.
Gunasekera
15
A11 11 Study of Spring Wheat Varieties for Identification of Forms Resistant
to Cadmium
R.A. Alybayeva, M.F. Mulyukova, A.E. Bektiyar, V.I. Kruzhayeva,
S.D. Atabayeva and S.Sh. Asrandina
16
xi
A12 12 Antioxidant and Glycemic Regulatory Properties of Blue Pea
(Clitoria ternatea L.) Flower Extract
S.A.T. Lakshan, N.Y. Jayanath, W.P.K.M. Abeysekera and W.K.S.M.
Abeysekera
17
A13 13 Enhanced Production, Purification and Characterisation of Industrial
Enzyme by Penicillium lilacinum (Thom, 1910)
K. Ahmed
18
A14 14 Polyamine and Mycorrhiza Based Mitigation of Cadmium Toxicity
in Sorghum vulagre L.
K. Prasann and D. Padmanabh
19
B1 15 Characterization of Plant Growth-Promoting Bacteria (PGPB)
Isolated from the Rhizosphere of Alfalfa (Medicago sativa L.)
M.M.A. Khan , A. El Omri , S.A. Kabli , A. Bahieldin , and S.M.S. Al-
Garni
20
B2 16 Environmental Risk Factors for Bacteriological Contamination in
Urban Drinking Water Wells in Jaffna Municipal Council Area
S.Sivathasan, S. Saravanan and K.Gunalan
21
C1 17 In Silico Construction and Structure Validation of Humanized
Aldolase Catalyitic Antibody 38C2
J.A.L.I. Sampath, A.N.L. Japahuge and R.S. Jayakody
22
C2 18 iOMICS: New Paradigm in Algal Biotechnology
P.P. Jutur
23
GENERAL BIOLOGICAL ENGINEERING (C)
(C
BIOMEDICINE ENGINEERING (B)
(
xii
C3 19 Whether Mankind Has a Chance of Succour from the Plant Kingdom
in Neurodegeneration? An In-Silico Investigation
S. Thiruchittampalam and S. Weerasinghe
24
C4 20 Proving the Efficacy of Mangiferin as a Neuroprotective Drug Using
Docking Studies
P. Singh, R. Samadarsi and D. Dutta
25
C5 21 Identification and In-Sillico Characterization of a Novel Hindiii
Isoschizomer from a Pseudomonas spp
S.N.J. Pathirana, M. Kajan, D.A.S. Elvitigala, G.H.C.M.
Hettiarachchi, C.M. Nanayakkara, R.G.S.C. Rajapakse and N.V.
Chandrasekharan
26
P1 22 Determination of Desirable Attributes of a Member of Burkholderia
cepacia Complex as a Biological Control Agent of Plant Pathogenic
Fungi
V.N.D. Bulathsinhalage and D.M. De Costa
28
P2 23 Molecular Characterization of Genus Puntius Using Mitochondrial
Gene Markers from Rivers of Southern Western Ghats, India
A.A. Arunkumar
29
P3 24 Development of Microbial Inoculum to Enhance the Degradation of
Organic Waste
J.K.A. De Silva, G.A.U. Jayasekera and C.M. Nanayakkara
30
P4 25 Enhanced Production of Bacillus thuringiensis Subspecies israelensis
Delta Endotoxin by Supplementation of Rotten Mango Juice and
Fish-Amino Acid as Medium Supplements
C. Gopinathan
31
P5 26 Rheological Properties and Antioxidant Activity of High Pressure
Processed Citrus Juices
S. Karlović, T. Bosiljkov, F. Dujmić, F. Troha, M. Škegro, M. Marelja,
32
POSTER PRESENTATIONS (P)
xiii
M. Brnčić and D. Ježek
P6 27 Benign Fabrication of Copper Nanoparticles from Flower Extract of
Cassia auriculata L
G.P. Chandran, T. Priyadharshini, C. Rajkuberan, N. Sekar and D.S.
Kumar
33
P7 28 0
2
Biosynthesis of Silver Nanoparticles Using Leaf Extract of Zingiber
officinale (Ginger), Allium cepa var. Aggregatum (Shallot), Capsicum
annuum (Bird’s eye chili) and Curcuma longa (Turmeric):
Determination and Comparison of Antioxidant, Antimicrobial
Properties with Leaves Water Extraction
Y.D.B. Peramuna and M. Kandiah
34
P8 29 An Iridoid Glycoside from the Sri Lankan Medicinal Plant
Wendlandia bicuspidata
S. Rajendran, S. Mohotti, E.D. de Silva, U. Göransson, S. Gunasekera
and C.M. Hettiarachchi
35
P9 30 The Application of Surfactants in Volatile Organic Compound (VOC)
Removal
L. Haddad, Z. Shareefdeen and H. Ahmed
36
P10 31 The Study of Genetic Variability in Related to Efficiency of Zinc
Optake in Different Genotypes of Triticum Aestivum L
A. H. Ghorttapeh, A. Majidi, J. Mozafari and A. Zyaeian
37
32 Stock Identification and Some Biological Aspects of Frigate Tuna
(Auxis thazard) in Sri Lankan Waters
D.R. Herath, U. Seneviratne, H.A.C.C. Perera and G.H.C.M.
Hettiarachchi
39
VIRTUAL PRESENTATIONS
xiv
33 Production of Protein Hydrolysates from Fish Skin for Dairy Products
E.I. Titov, G.V. Semenov, I.I. Ionova, I.S. Krasnova and N.S. Kozlov
40
34 Influence the Parameters of Freeze Drying on Antioxidant Activity of
Yoghurts with Different Tropical Fruits
G.V. Semenov, I.I. Ionova, Z.V. Volokitina and I.S. Krasnova
41
35 Influence of High Hydrostatic Pressure on Physical Properties of
Apple Juice
T. Bosiljkov, K. Klas, S. Karlović, F. Dujmić, M. Škegro, M. Marelja,
M. Brnčić and D. Ježek
42
36 Degradation of 2,4,6 TNT by Native Microorganisms Isolated from
TNT Contaminated Soil
M. Devasena and A. Thenmozhi
43
37 Multi Features Clustering Approach to Determine Parkinsonian
Disease upon Gait Movement Artifacts
S. Anujan and M. S. Karunarathne
44
38 Ultrasonic - Assisted Extraction of Bioactive Compounds from Waste
in Production of Red Wine
F. Dujmić, S. Karlović, T. Bosiljkov, F. Troha, M. Škegro, M. Marelja,
D. Ježek, R. Vidrih and M. Brnčić
45
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 1
KEYNOTE ABSTRACTS
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 2
NEW CHALLENGES FOR MAMMALIAN EXPRESSION SYSTEMS AND CELL
ENGINEERING STRATEGIES APPLIED TO OVERCOME THESE
J. Budge
University of Kent, United Kingdom
ABSTRACT
Recombinant biotherapeutic protein production from cultured mammalian cells has become an
established technology in the last 30 years. Adalimumab (Humira) was the first humanized
monoclonal antibody molecule generated using a mammalian host to attain FDA approval (tPA was
first biotherapeuitc with approval made in mammalian cells) and, since then, mammalian cells have
been employed to produce an array of different recombinant biotherapeutic protein molecules.
Chinese hamster ovary (CHO) cells have become the most widely used industrial mammalian host to
generate biotherapeutic proteins as they are robust and capable of folding, assembling and secreting
complex, multi-domain molecules with human-like post translational modifications. However, 18 of
the top 20 biggest selling recombinant therapeutics in 2014 attained FDA approval over a decade ago
and parts of the commercial focus of the industry is moving away from the development of single
high selling products and towards more bespoke drug discovery processes and subsequent synthesis.
Furthermore, many of the molecules on the market are monoclonal antibodies but an increasing
number of novel format molecules are being developed that bring about new challenges for previously
established host cell lines. Thus, in order to address the demands of a dynamically changing market it
is important to establish expression platforms necessary for both drug discovery, and subsequently
manufacturing, processes. Presented here is an approach to improve transient gene expression through
improved DNA delivery into CHO cells, and in combination with the induction of an extra
chromosomal maintenance system, achieve transient yields greater than either individually. It is
equally important to develop production platforms which have the capacity to generate challenging,
novel format molecules. Also presented here is a cell engineering approach which has proved
successful in improving yields and product quality of difficult-to-express molecules in CHO cells.
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 3
MOLECULAR MECHANISMS OF YEAST CELL WALL BIOSYNTHESIS AND
HOW CAN WE USE IT TO ENGINEER THE SURFACE OF THE CELL
V. Mrša, A. Sk. Hossain, A. Grbavac, M. Lozančić M and R. Teparić
Laboratory of Biochemistry, Faculty of Food Technology and Biotechnology, University of
Zagreb, Croatia
ABSTRACT
Exploring microbial cell walls and particularly molecular mechanisms of wall protein localization
opened new possibilities of surface display of homologous and heterologous proteins. By fusing
anchoring domains of surface proteins with biotechnologically interesting proteins their genetic
immobilization is achieved. Hybrid proteins are expressed in the host cells, secreted to the cell surface
and incorporated into the cell wall. In this way chemical immobilization of proteins to a solid matrix
is avoided by using production cells as matrices themselves. Both bacteria and yeasts have been used
for this purpose and a number of potential biotechnological applications of surface displayed proteins
from whole cell biocatalysts, to biosorbents and biosensors have been reported. When surface
immobilized enzymes are used, substrates do not need to cross membrane barriers, i.e. enzymes can
freely access any externally added substrate. Thus, enzyme purification is bypassed and multi-step
transformations can be achieved using cells displaying enzymes catalyzing cascade reactions.
Particularly interesting are yeast systems for surface display since most yeasts are GRAS
microorganisms, their cell walls bind more proteins, and cells are bigger. Besides, yeasts are generally
more suitable for expression of proteins from higher eukaryotes. However, surface display in yeast
cells is still limited by our comprehension of mechanisms of cell wall biosynthesis and by insufficient
understanding of their regulation. An overview of yeast cell walls biosynthesis, as well as the
regulatory mechanisms involved will be presented. Applications of gained knowledge in the
development of the surface display technology will be discussed on several examples.
Keywords: yeast cell walls, cell wall biosynthesis, surface display, genetic immobilization
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 4
ARABIDOPSIS TO RICE: APPLYING KNOWLEDGE GAINED FROM MODEL
PLANT TO ENHANCE AGRICULTURALLY IMPORTANT CROP PLANTS
C. Hettiarachchi
Department of Chemistry, University of Colombo, Sri Lanka
ABSTRACT
Light is of utmost importance to sessile plants, not only as the primary energy source for
photosynthesis, but also as an environmental signal, regulating their growth and development.
CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1) and ELONGATED HYPOCOTYL 5
(HY5) are two key regulators of plant light signaling pathway. COP1, an E3 ubiquitin ligase,
represses light dependent development by targeting positive regulators such as the transcription factor
HY5 for degradation in the dark. BBX21/ SALT TOLERANCE HOMOLOG 2 (STH2) is a B-box
encoding protein that interacts with both COP1 and HY5 in photomorphogenesis. BBX21 protein can
activate light dependent transcription and positively regulate the light mediated development of
plants.
It has been shown; the productivity of crop plants might be enhanced by over-expressing central
regulators of light signaling pathway. Bg 250 Sri Lankan rice variety was transformed with
Arabidopsis BBX21 gene via Agrobacterium mediated transformation to improve its quality and the
productivity. The T0 Bg 250 putative transgenic plants were screened for the presence of BBX21
gene by PCR and the PCR positive rice plants were further grown to obtain seeds. Transcript analysis
was conducted with homozygous T3 BBX21 transgenic plants and the results showed strong
expression of BBX21 gene in transgenic BG 250 plants compared to wild type Bg 250 plants.
Phenotypic studies of BBX21 T3 plants showed increased plant height, leaf length, leaf area, number
of panicles and bushy appearance due to increased number of tillers when compared with non-
transgenic plants.
Light stimulates germination of Arabidopsis seeds whereas the phytohormone Abscisic acid (ABA)
inhibits germination. BBX21 is a positive regulator of the light signals and appear to act as a negative
regulator of ABA signals suggesting that this protein might integrate these opposing signaling
pathways in plants. Genetic and expression analysis of double mutants between bbx21 and four key
regulators of ABA signaling showed that the expression of key regulators of ABA signaling is altered
in the bbx21 mutant indicating that the BBX21 gene plays an important role in response to drought
and salinity. At present research is being conducted to apply this knowledge to obtain drought and
salinity tolerant Bg 250 that could give higher yields per Hectare per year.
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 5
ORAL PRESENTATIONS
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 6
A1 [01]
DEVELOPMENT OF E. Coli PLATFORMS FOR DITERPENE SYNTHESIS
M. Fuchs
Technical University of Munich, Germany
ABSTRACT
The production of terpenoide drugs is often performed by a chemical (semi-)synthetic process
resulting in toxic chemical waste streams. Our research focuses on the development and optimization
of microbial production platforms for structurally minimized derivatives or artificially designed
compounds for pharmaceutical and agrochemical applications. Our processes do not affect land use
and are free of toxic waste streams. Hitherto, we have established a recombinant E. coli strain
carrying MEP pathway derived genes for IPP/DMAPP biosynthesis, which can be expanded with
different terpene cyclases. Based on molecular modeling terpene cyclases are engineered for the
production of new ring systems to get new compounds not only with anticancer but also with
antibiotic or anti-inflammatory effects. The taxadiene synthase, which forms the first dedicated
intermediate in taxol biosynthesis, taxadiene was engineered to produce verticillene, which also has
reported anticancer activity. The new terpenoide ring systems were further functionalized for
enhanced biological efficiency. The hydroxylation via lipases or stereo selective hydroxylases are
established possibilities is to attach acceptor functionalities for downstream acetylation or
benzoylaton. This was performed in a one-cell process or on purified terpenoid skeletons. In iterative
steps a systems biology approach improved the production strain. Here the product isolation from
fermentation media is a challenging task. In proof-of-concept studies product removal by extend
application of known cleaning strategies was tested using stationary adsorbers and counter current
chromatography methods.
Keywords: E. coli, diterpene drugs, functionalization, purification
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 7
A2 [02]
FUNCTIONAL AND STRUCTUAL CHARACTERIZATION OF Setaria digitata
NOVEL PROTEIN (SdNP)
M. Gowthami1, K.Varmila1, Gayathri. N. Silva1 and R.S. Dassanayake1
1Department of Chemistry, Faculty of Science, University of Colombo, Colombo, Sri Lanka
ABSTRACT
Wuchereria bancrofti, a human parasitic nematode, is the major causative agent of “lymphatic
filariasis”. Studying the parasitology of W. bancroft iis highly challenging due to the difficulties
inobtaining an adult parasite from the lymphatic system. Therefore, Setaria digitata, a bovine filarial
parasite is used as a model organism due to its close resemblance to W.bancrofti and other filarial
nematodes. Setaria digitata novel protein (SdNP) was identified from S. digitata, that is uniquely
expressed in the parasitic nematodes. Sequence analysis and bioinformatics studies done on SdNP
revealed the presence of two partial ATPase domains and potential sites for phosphorylation. The
research work presented here describes in-vitro functional and structural characterization of SdNP.An
enzyme coupled ATPase assay was used to detect the ATPase activity of SdNP and the results show
that SdNP hydrolyses ATP to ADP and inorganic Pi in a substrate-independent manner. Pro-Q
diamond phosphor protein dye was used to detect the phosphorylation state of the protein and the
results confirm that SdNPis a phosphor-protein. In addition, native-PAGE and gel-filtration
chromatography were carried out to analyze the oligomerization state of SdNP. The results obtained
show that SdNP is forming a stable oligomer (approximately a tetramer).Overall, the results confirm
that SdNP is a tetramer and a phosphoprotein that has the ability to bind and hydrolyze ATP. Studying
the structure and function of SdNP is of great importance because SdNP is only found in pathogenic
nematodes and can be used as a potential target to develop clade-specific drugs against lymphatic
filariasis.
Keywords: Setaria digitata, SdNP, ATPase activity, Oligomer, Phosphorylation
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 8
A3 [03]
ANALYSIS OF PAPILLARY THYROID CARCINOMA ASSOCIATED
BIOMARKERS AND GENETIC ABNORMALITIES TO DETERMINE THE RISK
OF DEVELOPING CANCER IN PATIENTS DIAGNOSED WITH DIFFERENT
THYROID DISORDERS
P. Shirani1, T. Dhanushika1, F.I. Moulana, A.A.H. Priyani 2, M.C.V. de Silva2 and
R.S. Dassanayake 1
1Department of Chemistry, Faculty of Science, University of Colombo, Colombo, Sri Lanka
2Department of Pathology, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka
ABSTRACT
Thyroid disorders such as multinodular goiter (MNG) and Hashimoto’s thyroiditis (HT) leading to
papillary thyroid carcinoma (PTC) is a subject of controversy that prevails in the scientific
community. This prospective study investigates correlation between these disorders and PTC by
analyzing NRASQ61R, which is one of the most prevalent point mutation in PTC, and also the
expression levels of thyroid-stimulating hormone receptor (TSHR), manganese superoxide dismutase
(MnSOD) and a receptor tyrosine kinase (c-RET) in microscopic foci with similar nuclear features for
PTC in patients with HT and MNG. A total of 22 formalin-fixed paraffin embedded (FFPE) tissue
samples were subjected to polymerase chain reaction-restriction fragment length polymorphism
(PCR-RFLP) to analyze NRASQ61R mutation, of which 18 FFPE samples were subjected to real-time
PCR relative quantification to analyze the gene expression levels in the test regions compared to the
selected control regions. In the detection of NRASQ61R mutation, 95.5% of samples tested were
positive for the mutation in both test and control areas. In the quantification of mRNA, 61% of
samples showed an elevation in TSHR levels, 78% in MnSOD levels while 50% in c-RET levels in the
test areas relative to the control areas. Elevation of mRNA levels suggests that there is a higher
potential of patients with MNG and HT to develop PTC. This is further supported by the presence of
NRASQ61R point mutation in most of the samples analyzed.
Keywords: MnSOD, c-RET, TSHR, NRASQ61R, PCR-RFLP, HT
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 9
A4 [04]
BEHAVIORAL AND BIOCHEMICAL EFFECTS OF BANANA (Musa sapientum L)
PEEL EXTRACT FOLLOWING NOISE STRESS IN MALE MICE
N. Samad and A. Muneer
Department of Biochemistry Bahauddin Zakariya University, Multan-Pakistan
ABSTRACT
The present study was aimed to investigate the anti-anxiety, antidepressant and memory enhancing
effects of banana (Musa sapientum L.) peel extract following acute noise stress in male mice. Locally
bred albino Wistar mice were divided into control and test groups (n=12). Control mice were received
drinking water while test groups were treated with banana peel extract (400 mg/kg; oral
administration). Animals were received their respective treatment for 14 days. Animals were
subdivided (n=6) into unstressed and stressed groups on day 15. Stressed groups were subjected to
noise stress for 4-h. Behavioral activities of animals were monitored 24-h after the 4-h noise stress.
Depression-like symptoms were measured by forced swimming test (FST). Anxiety like behavior was
monitored using light–dark transition test and plus maze activity test and memory functions of mice
were assessed by morris water maze (MWM) test. 1-h after the post administration of respective
treatment animals were decapitated and brain was removed for estimation of antioxidant enzymes
[catalase (CAT), superoxide dismutase (SOD), reduced glutathione (GSH)], acetylcholinestrase
(AChE) and oxidative stress marker malondialdehyde (MDA). In the present study banana peel
attenuated stressed-induced behavioral deficits and produced anxiolytic and antidepressant-like
effects. An increase in memory performance was also observed in banana peel treated animals. The
activities of all antioxidant enzymes were significantly greater in banana peel treated unstressed and
stressed animals while activity of AChE is decreased in unstressed animals. Conversely, banana peel
extract reduced the levels of MDA in both unstressed and stressed groups than control animals. It is
concluded that banana peel have anti-anxiety, antidepressant effect as well as strengthen the memory
possibly via its antioxidant mechanism following acute noise stress. Therefore, it is recommended that
supplementation of banana peel could be beneficial for the treatment of anxiety, and depression and
improve memory function.
Keywords: Banana peel, noise stress, depression, anxiety, memory, antioxidant enzymes
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 10
A5 [05]
PHYTOREMEDIATION POTENTIAL OF LEGUME PLANT Cajanus cajan
(PIGEON PEA) ON PETROLEUM OILY SLUDGE CONTAMINATED SOIL
I. A. Allamin1,2, S.A. Ahmad1, N.A. Yasid1, S.R.S. Abdullah3, M.I.E. Halmi4 and
M.Y. Shukor1
1Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences,
Universiti Putra Malaysia, Selangor, Malaysia
2Department of Microbiology, Faculty of Sciences, University of Maiduguri, Maiduguri,
Nigeria
3Department of Chemical and Process Engineering, Faculty of Engineering and Built
Environment, Universiti Kebangsaan Malaysia, Malaysia
4Department of Land Management, Faculty of Agriculture, University of Putra Malaysia,
Malaysia
ABSTRACT
A considerable amount of polluti is generated from petroleum industry during exploration,
production, transportation, storage, and refining processes. Most components of petroleum
hydrocarbon disposed or spilled to the environment are toxic, mutagenic and cancer-causing. There is
the need of effective and ecofriendly remediation measures. Thus, research in phytoremediation. C.
cajan a legume plant was screened on petroleum oily sludge contaminated soil. Plant tolerance to the
contaminant was monitored and determined (plant height, dry biomass and RGR). Rhizosphere soil
was sampled to determined (total heterotrophic bacteria (THB) using nutrient agar, nitrogen fixing
bacteria (NFB) using yeast extract mannitol agar, and hydrocarbon utilizing bacteria (HUB) using oil
agar). Degradation rates were estimated gravimetrically and quantified on GC-FID. Accumulation of
heavy metal and root exudates released by C. cajan was determined using AAS and HPLC
respectively. The response of the plant to oily sludge varied significantly to the untreated control and
among the treatments. The growth of C. cajan was slightly (but not significantly) influenced by the
oily sludge in soil; growth of C. cajan in relatively lower concentrations of petroleum oily sludge
slightly greater to the untreated control without petroleum oily sludge. A significant interaction was
observed for relative growth rates (RGRs) biomass of C. cajan. The removal of total petroleum
hydrocarbon (TPH) after 90 days in treatments with 1%, 2% and 3% shows 92%, 90% and 89%
respectively and 68.3% and 47.3% in relatively higher concentrations 4% and 5% respectively. As the
search for more eco-friendly and sustainable remediating green plant continues, C. cajan shows a
great potential in reclaiming petroleum oily sludge-contaminated soil. Our findings will provide
solutions to polluted soils and subsequent re-vegetation.
Keywords: Oily sludge, Cajanus cajan, phytoremediation and legume-microbe
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 11
A6 [06]
RESPONSE SURFACE-BASED OPTIMIZATION AND KINETIC STUDIES OF
PHENOL DEGRADATION BY Alcaligenes SP. AQ5-02
A. Aisami 1,2, N.A. Yasid1, W.L.W. Johari3, S.A. Ahmad1 and M.Y. Shukor1
1Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences,
Universiti Putra Malaysia, Malaysia
2Department of Biochemistry, Gombe State University, Nigeria
3Department of Environmental Sciences, Faculty of Environmental Studies, Universiti Putra
Malaysia, Malaysia
ABSTRACT
In Malaysia, phenol and phenolic compounds rank among the top three scheduled wastes with
thousands of tonnes being produced yearly for disposal. In Malaysia about 37.7 metric tonnes of
phenol and phenol-containing wastes are produced in 2014, there is also an incident of tanker
accidents Straits of Malacca in 2003 where tonnes of phenol were spilled. Bio-removal of phenol by
microorganisms especially bacteria has been demonstrated to be the most effective and economical
approach compared to physio-chemical methods. In this study, a phenol-degrading Alcaligenes sp.
AQ5-02 has been isolated from a contaminated area. Optimisation using Response Surface
Methodology showed that the highest degradation was achieved at pH 7.5 (phosphate buffer), 0.45
g/L ammonium sulphate and an optimum temperature at 30°C. Growth kinetic modelling of the
bacterium on phenol shows that the best model was Luong with the lowest values for root mean
square error or RMSE and adjusted Akaike Information criteria AICc, highest adjusted R2 values, and
with Bias Factor and Accuracy Factor nearest to unity (1.0). The calculated value for the Luong’s
constants maximal growth rate, half saturation constant for maximal growth, maximal concentration
of substrate tolerated and curve parameter that defines the steepness of the growth rate decline from
the maximum rate, symbolized by umax, Ks, Sm, and n were 0.07±0.01 hr-1, 0.18±0.03 g/L, 1.27±0.24
g/L and 6.60±0.94 for Alcaligenes sp. AQ05-02. Phenol was degraded through the meta pathway.
Keywords: Phenol, Alcaligenes sp., Response Surface Method, Luong, meta pathway
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 12
A7 [07]
ISOLATION AND IDENTIFICATION OF CADMIUM SENSING REGULATOR –
CADC FROM Bacillus megaterium STRAIN TWSL-4
W.M.N.H. Kumari, C.D. Wijayarathna and N.V. Chandrasekharan
Department of Chemistry, Faculty of Science, University of Colombo, Sri Lanka
ABSTRACT
In the search for an eco-friendly solution to decontaminate toxic heavy metals existing in industrial
effluents, a bacterial strain designated as TWSL-4 with metal ion (Cu2+, Cd2+ and Pb2+) resistance and
removal ability was isolated and identified up to the species level (B. megaterium) using 16S rRNA
gene analysis. Initial studies revealed the presence of genes involved in cadmium ion efflux
mechanism (P-type ATPases/cadA) in this bacterium. The current study was focused on finding genes
related to the cad operon. The upstream region of the cadA gene was amplified and cloned into a
pGMET vector. The 375 bp insert was sequenced. BLASTn analysis revealed the sequence to be 99%
homologous to the cadC gene (cds=119 aa) which codes for the Cadmium efflux system accessory
protein (CadC) of B. megaterium and 72% homologous to the cadC gene (cds=121 aa) of
Staphylococcus sp. BLASTp analysis showed 100% homology to ArsR family transcriptional
regulator of Bacillus sp. Protein sequences of both species shared similar features although protein
homology was around 70%. However the amino acids involved in binding at the ion binding site
(amino acids 57, 60 and 102) are conserved confirming functional similarity. Phylogenetic analysis of
the cadC gene revealed it to be highly related to the cadC gene of Bacillus sp. rather than
Staphylococcus sp. reflecting the BLAST search results. Overall results revealed the isolated gene to
code for cadmium sensing regulator with three sites; dimerization interface [polypeptide binding],
putative DNA binding site [nucleotide binding] and putative Zn2+ binding site [ion binding].
Keywords: heavy metals, industrial effluents, Bacillus megaterium, cad operon, cadC
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 13
A8 [08]
EFFICIENT HETEROTROPHIC MANGROVE BACTERIA FOR CARBON
SEQUESTRATION THROUGH MICROBIALLY INDUCED CALCIUM
CARBONATE PRECIPITATION (MICP)
N.V. Kumar, P. Ammini and J. Vijayan
National Institute of Oceanography-CSIR, Regional Centre, Dr. Salim Ali Road, India
ABSTRACT
Microbially induced calcium carbonate precipitation (MICP) is a natural bio-mineralization process
induced by microorganisms involving bacterial extracellular enzymes. Carbonic anhydrase and urease
are the major enzymes playing major role in the process of MICP. In this study, 15 bacterial colonies
(PV1-PV15) were isolated from mangrove sediments of Puthuvype, Kerala, India. The isolates were
screened for their carbonic anhydrase and urease enzyme production based on p-Nitro phenol acetate
assay and urease agar method respectively. The positive isolates were subjected to secondary
screening through quantitative method. Among them, a potential isolate was identified as
Virgibacillus halodenitrificans PV15 based on 16S rDNA sequencing. The isolate exhibited 40 U mL-
1 of carbonic anhydrase and 731.91 U mL-1 of urease activity. The enzyme kinetics was studied based
on Line weaver-Burk plots. The calcium precipitation assay to determine the carbon sequestration
ability of the microbial isolate showed a reduction of 72% of free calcium in the medium through
calcite precipitation. The carbonic anhydrase and urease had a synergistic effect in MICP. CA and
urease mediated calcium precipitation by V. halodenitrificans PV15 could contribute towards the
process of carbon dioxide sequestration. This isolate could be used for effectively for the carbon
dioxide sequestration application. Moreover, the enzyme mediated bio-mineralization is one of the
most economical methods for mitigating global warming.
Keywords: carbon sequestration, bacteria, carbonic anhydrase, urease, mangroves
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 14
A9 [09]
siRNA MEDIATED RNA INTERFERENCE OF NOVEL PARASITIC NEMATODE-
SPECIFIC PROTEIN OF Setaria digitata IN Culex quinquefasciatus SHOWED
MOLTING AND MOVEMENT DETECTS AND DEVELOPMENT ARREST
M.B.C.L. Somarathne
University of Colombo, Sri Lanka
ABSTRACT
Functional studies of animal parasitic nematodes at molecular level are being held back due to the
scarcity of available nematode culturing methods and gene manipulation tools. Therefore this study
was undertaken to utilize novel in-vivo culturing method in mosquito together with RNA interference
post transcriptional gene silencing tool in functional analysis of Setaria digitata nematode specific
gene (SDNP). Culex quinquefasciatus mosquitoes, the natural vector of the parasite were infected
with S. digitata microfilariae by micro-injection. RNAi treatment was executed with the introduction
of in-vitro synthesized Cy3 labeled siRNA in to the infected mosquitoes. Following the RNAi
treatment, red fluorescence was observed inside the larvae, indicating the uptake of siRNA. q-PCR
analysis demonstrated a significant reduction in the transcript (90% (P < 0.05) levels of SDNP in the
RNAi treated larvae compared to the non-treated, suggesting the RNAi pathways are operational in
the parasite. The resulted motility reduction, molting deformities (77% reduction in L2-L3
transformation) and the irregularities in the migration of the parasite in the mosquito followed by the
RNAi, pointed out the involvement of SDNP in locomotion and growth of the worm. Furthermore,
migration irregularities and increased mosquito survival in the test group eventually resulted in a
dramatic decrease in the parasitism of the larvae. Collectively, this study demonstrates the successful
utilization of mosquito as an in-vivo culture platform and RNAi as a robust tool in gene manipulation.
Moreover, according to the characteristics of SDNP, it can be suggested that it has a potent
candidature as a novel anthelminthic drug target.
Keywords: S.digitata, RNAi, in-vivo culturing, SDNP
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 15
A10 [10]
HIGH THROUGHPUT SCREENING OF BIOACTIVE SECONDARY
METABOLITES IN SRI LANKAN MEDICINAL PLANTS BY
MICROFRACTIONATION AND TARGETED ISOLATION OF ANTIBACTERIAL
FLAVONOIDS
S. Rajendran 1,2 , S. Mohotti1,2, T. Muhammad1, A.A. Strömstedt1, A. Adhikari3, E.D. de
Silva2, U. Göransson1, C.M. Hettiarachchi2 and S. Gunasekera1
1Division of Phamacognosy, Department of Medicinal Chemistry, Uppsala University,
Biomedical Centre, Sweden
2Department of Chemistry, Faculty of Science, University of Colombo, Sri Lanka
3International Centre for Chemical and Biological Sciences, H. E. J. Research Institute of
Chemistry, University of Karachi, Pakistan
ABSTRACT
Sri Lankan Ayurvedic system of traditional medicine derives from plant-based remedies handed down
from generation to generation over a period of 3000 years. Despite being an integral part of the Sri
Lankan culture, the scientific evidence for the biological activity of these traditional medicines are
limited and there’s a lack of scientific evidence for efficacy, toxicity, standardization of formulations
as well as adverse interactions of the medications. The current project was conducted to identify and
characterize chemical compounds present in selected Sri Lankan medicinal plants belonging to the
family Fabaceae, which have potential utility as therapeutic candidates for antimicrobial applications.
The medicinal plants were chosen based on their ethno pharmacology values. Organic crude extracts
of selected plants were subjected to agar disc diffusion assay against Staphylococcus aureus, Bacillus
cereus, Pseudomonas aeruginosaand Escherichia coli. Aqueous extracts of plantswere also subjected
to antibacterial activity screeningby a microfractionation protocol. Each aqueous crude plant extract
was fractionated into a 96 deep well micro titer plate using analytical HPLC (2 mg of crude extract to
obtain 45 x 1 ml fractions) and the fractions were screened against S. aureusin an optimized Minimum
Inhibitor Concentration assay. The corresponding masses in the bioactive microfractions were then
identified by liquid chromatography coupled to mass spectroscopy.Among the tested plants, Derris
scandens showed the highest activity against Staphylococcus aureus. D. scandenswas then subjected
to aactivity/mass guided fractionation protocol to isolate corresponding masses in the bioactive
fractions; four pure flavonoids (Dalpanitin, Rutin, Vicenin2 and Vicenin3) were isolated and their
structures were elucidated by NMR. Pure compounds were also subjected to biological assays in order
to confirm their antibacterial activity.
Keywords: Derris scandens, microfractionation, Traditional medicine, MIC, LCMS
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 16
A11 [11]
STUDY OF SPRING WHEAT VARIETIES FOR IDENTIFICATION OF FORMS
RESISTANT TO CADMIUM
R.A. Alybayeva, M.F. Mulyukova, A.E. Bektiyar, V.I. Kruzhayeva, S.D. Atabayeva and
S.Sh. Asrandina
Kazakh National University named after al-Farabi, Almaty, Republic of Kazakhstan
ABSTRACT
The purpose of our study was the identification of spring wheat varieties resistant to cadmium, the
priority pollutant in the East Kazakhstan region. The experiments were carried out in the condition of
model contamination of the medium on 14-day sprouts of various varieties of spring wheat from the
collection of the Kazakh Research Institute of Farming and Plant Growing grown on a nutrient
mixture containing 0.1 mM CaSO4 and Cd ions at a concentration of 40 mg/l (as a CdSO4 salt).
Screening of varieties of spring wheat under laboratory conditions made it possible to identify stable
and sensitive genotypes. In terms of growth, accumulation of cadmium in the aerial organs the most
resistant to cadmium are the varieties of spring wheat Samal and Kayyr, the least resistant are the
varieties Lutescens and Zhenis. In terms of growth, accumulation of cadmium in the roots the most
resistant varieties were spring wheat Kazakhstanskaya-15 and Kazakhstanskaya Early, the most
unstable - the variety Lutescens. A slight increase in the maintenance of chlorophyll a and b in the
presence of cadmium in most varieties was revealed. The fact of a smaller variability in the ratio of
a/b chlorophylls in more resistant to cadmium translocation to the aerial organs of genotypes is
revealed, compared with more sensitive ones. Cadmium-resistant varieties can be used in field studies
of soil contamination with cadmium to identify promising forms that combine metal resistance with
high yield and resistance to weather conditions.
Keywords: cadmium, spring wheat, growth parameters, cadmium content, chlorophyll content,
sensitive and resistant genotypes
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 17
A12 [12]
ANTIOXIDANT AND GLYCEMIC REGULATORY PROPERTIES OF BLUE PEA
(Clitoria ternatea L.) FLOWER EXTRACT
S.A.T. Lakshan1, N.Y. Jayanath2, W.P.K.M. Abeysekera3 and W.K.S.M. Abeysekera4
1,2University of Peradeniya, Sri Lanka
3Industrial Technology Institute, Sri Lanka
4University of Colombo, Sri Lanka
ABSTRACT
Flower of Clitoria ternatea is reported to have various biological activities world over. However,
there is no single study conducted in Sri Lanka on biological activities of this plant. This study
investigated antioxidantand glycemic regulatory properties of Clitoria ternatea flower. Hot water
extract of Clitoria ternatea blue flower was used in this study. Pre-optimized conditions of
temperature (59.6 ºC), time (37 min), and flower: water ratio (3 g/L) were used in extraction. In- vitro
antioxidant properties were investigated in terms of total phenolic content (TPC), total flavonoid
content (TFC), ferric reducing antioxidant power (FRAP), oxygen radical absorbance capacity
(ORAC) and DPPH and ABTS+ radical scavenging activities. Glycemic regulatory properties were
investigated using in vitro anti-amylase and anti-glucosidase assays. TPC, TFC, FRAP, ORAC, DPPH
(IC50) and ABTS+(IC50) radical scavenging activity antioxidant properties of the flower extract were
26.72±2.17 mg gallic acid equivalents/g, 14.25±0.58 mg quercetin equivalents/g, 5.13±0.54 mg trolox
equivalents (TE)/g, 36.73±1.06 mg TE/g, 45.31±1.47 μg/mL, and 6.74±0.10 µg/mL respectively.The
observed antioxidant properties were moderate compared to the reference standards used. Inhibition
of α-amylase by the flower extract was low (4.28±1.02 %) whereas no anti-glucosidase activity was
observed at the tested concentrations. In conclusion, the water extract of blue Clitoria ternatea flower
had antioxidant properties via various modes of actions with mild anti-amylase activity.
Keywords: Antioxidants, Glycemic regulatoryproperties, Blue pea flower, Hot water extract
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 18
A13 [13]
ENHANCED PRODUCTION, PURIFICATION AND CHARACTERISATION OF
INDUSTRIAL ENZYME BY Penicillium lilacinum (THOM, 1910)
K. Ahmed
Department of Chemistry, NED University of Engineering & Technology, Karachi, Pakistan
ABSTRACT
Recent years researchers have been motivated towards extensive exploring of living organism, which
could be utilized effectively in intense industrial conditions. The present study shows enhanced
production, purification and characterization of industrial enzyme, invertase (Beta-D-
fructofuranosidase) from Penicillium lilacinum (Thom, 1910). Various agricultural based by-products
(cotton stalk, sunflower waste, rice husk, molasses and date syrup) were used as energy source. The
highest amount of enzyme (13.05 Units/mL) was produced when the strain was cultured on growth
medium containing date syrup as energy source, yeast extract as a source of nitrogen, after 96 h of
incubation, at incubation temperature 40º C, initial pH of medium 8.0, inoculum size of 6x106 conidia
and 200 rev/min of agitation rate. The enzyme was also purified (7 folds than crude) and
characterized. Molecular mass of purified enzyme (65 kDa) was determined by 10 % SDS-PAGE.
Lineweaver-Burk Plot was used to determine Kinetic constants (Vmax 178.6 U/mL/min and Km 2.76
mM). Temperature and pH optima were 55º C and 5.5 respectively. MnCl2 (52.9 %), MgSO4 (48.9
%), BaCl2 (24.6 %), MgCl2 (9.6 %), CoCl2 (5.7 %) and NaCl (4.2 %) enhanced the relative activity of
enzyme and HgCl2 (-92.8 %), CuSO4 (-80.2 %) and CuCl2 (-76.6 %) were proved inhibitors. The
strain was showing enzyme activity even at extreme conditions of temperature (up to 60º C) and pH
(up to 9) so it could be potential candidate for industries.
Keywords: Invertase, Penicillium lilacinum, Submerged fermentation, Industrial enzyme
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 19
A14 [14]
POLYAMINE AND MYCORRHIZA BASED MITIGATION OF CADMIUM
TOXICITY IN Sorghum vulagre L.
K. Prasann1 and D. Padmanabh2
1Department of Agronomy, School of Agriculture, Lovely Professional University, India
2Department of Plant Physiology, Institute of Agricultural Sciences, Banaras Hindu
University, Varanasi, India
ABSTRACT
Cadmium is one of the most hazardous heavy metals in the atmosphere, soil and aquatic system which
is finally going into our food chain and responsible for serious environmental problem leading to the
health hazards in the living organisms. Sorghum vulgare L. plants when exposed to cadmium nitrate
with the concentrations of 70ppm and 150ppm per kg of soil for 90 days, exhibited phytotoxic
responses. The observations of specific responses were dependent on treatment combinations. The
significant hazardous effects and oxidative damage of cadmium nitrate (70 ppm and 150ppm) were
evident by increased MDA content and hydrogen peroxide content. The metal tolerance and
detoxification strategy adopted by the plant was investigated with reference to a non enzymatic
antioxidant system (proline) and the antioxidant enzymes viz., Superoxide dismutage, Polypheol
oxidase, Peroxidase and catalase, which were found to be reduced due to cadmium stress. However,
these responses were reversed by exogenous application of putrescine (2.5 and 5.0 mM) and
mycorrhiza (Glomus; 150 inoculants per kg of soil), more so, in their combined treatment, at different
DAS. Peaks of alcoholic, aldehyde and ketone functional groups were sharper and strong in plants
grown in higher concentration of cadmium after the observation under FT-IR. Whereas plants treated
with putrescine and Glomus showed a decreasing trend of sharpness of peaks with respect to non
treated plants. Dispersion Scanning Calorimetric data showed decreasing trend of stability with
increasing concentration of cadmium. This was expressed in the terms of glass transition phase
temperature. But combined treatment of putrescine and mycorrhiza enhanced the stability of sorghum
by reducing the ROS production in plant cell. On the basis of the data obtained, it is concluded that
plants responded up to 70 ppm cadmium nitrate with stress induced responses which were ameliorated
by combined application of putrescine and Glomus mycorrhiza.
Keywords: agriculture, biotic, cadmium, density, energy
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 20
B1 [15]
CHARACTERIZATION OF PLANT GROWTH-PROMOTING BACTERIA (PGPB)
ISOLATED FROM THE RHIZOSPHERE OF ALFALFA (Medicago sativa L.)
M.M.A. Khan 1, A. El Omri 1, S.A. Kabli 1, A. Bahieldin 1, and S.M.S. Al-Garni 1
1Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Saudi
Arabia
ABSTRACT
Application of plant growth-promoting bacteria (PGPB) is an environmentally-friendly approach to
enhance crop production. However, indigenous PGPB are likely to be more adaptive and may serve as
better plant growth promoters. In this study, twenty five bacteria were isolated from the rhizosphere of
alfalfa plant growing in Jeddah, Saudi Arabia, and assessed for indole-3-acetic acid (IAA), 1-
aminocyclopropane-1-carboxylic acid (ACC) deaminase, and inorganic phosphate (Pi) solubilization
activity in vitro. Out of 25 bacteria, ten isolates produced significant amount of IAA in presence of L-
tryptophane. The maximum (85 μg/ml) IAA was synthesized by KB-10. Only KB-25 displayed all the
three tested PGP traits i.e., IAA (19 μg/ml), ACC deaminase (0.1 μmol α-ketobutyrate/mg/h), and Pi
solubilization activity. These two strains were further tested for exopolysaccharides, ammonia, and
hydrogen cyanide (HCN) production ability in vitro. Both KB-10 and KB-25 were found positive for
ammonia, but negative for exopolysaccharides and HCN production. They were gram-negative, rod-
shaped, motile, catalase and oxidase positive. The 16S rRNA genes of KB-10 and KB-25 showed
100% sequence homology with Pseudomonas pseudoalcaligenes and P. putida, respectively. Based
on their phenotypic, biochemical and 16S rRNA gene phylogeny, KB-10 and KB-25 were tentatively
identified as P. pseudoalcaligenes KB-10 and P. putida KB-25, respectively. Currently, a pot
experiment is going on to evaluate the effectiveness of these strains to improve growth and
productivity of alfalfa in nutrient-impoverished soil. This study reports two PGPB strains native to
Saudi Arabia which may have the potential to be used as bioinoculants for sustainable alfalfa
cultivation.
Keywords: PGPB, IAA, ACC deaminase, Pseudomonas, alfalfa
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 21
B2 [16]
ENVIRONMENTAL RISK FACTORS FOR BACTERIOLOGICAL
CONTAMINATION IN URBAN DRINKING WATER WELLS IN JAFFNA
MUNICIPAL COUNCIL AREA
S.Sivathasan1, S. Saravanan2 and K.Gunalan3
1,2National Water Supply and Drainage Board, Jaffna, Sri Lanka
3Deparment of Fisheries, University of Jaffna, Sri Lanka
ABSTRACT
The Jaffna Municipal Council area (JMC) lies in the Southern part of Jaffna Peninsula. The Jaffna
lagoon forms the Southern boundary of the area. The JMC consists of Jaffna Divisional Secretariat
Division and part of Nallur Divisional Secretariat Division. The objective of the study was to define
the presence of bacterial contamination of Ground water used for domestic purposes in Jaffna
Municipal council area. Ninety one well locations were selected to represent the JMC area water
quality, and the locations include domestic and common dug wells and analyzed in December 2014 to
February 2015 (during the raining seasons) and results were compared with Sri Lankan drinking water
quality standard (SLS 614:2013). The results showed of bacterial contamination in the water sources
means that some forms of treatment needed to be done before consumption. The mean count of total
coliform and faecal coliform ranged from 0 to 705 CFU/100 mL and 0 to 637 CFU/100 mL water
sample, respectively. Most of costal area brackish water wells from Gurunagar, Paasiyoor,
Navanthurai and Colombothurai also showed high fecal contamination. The Jaffna Town does not
have proper sewerage facilities and every household are having individual toilet with a open dug pit.
These open pits are the source of contaminant for the bacterial pollution. The most basic and
important factor is the short distance / setback distances between the source of pollution and the well.
Every household have individual well because of the non-availability of pipe born water supply and
most of the people are using the water for their day to day needs as well as Drinking water. For
adequate determination of setback distances, information on attenuation and transport of pathogens or
microbial indicators in groundwater systems are needed. In the larger interests of public health, the
implementation of a sewerage scheme for the city is absolutely necessary.
Key words: E.coli, T.coli, Jaffna Municipal Council
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 22
C1 [17]
In Silico CONSTRUCTION AND STRUCTURE VALIDATION OF HUMANIZED
ALDOLASE CATALYITIC ANTIBODY 38C2
J.A.L.I. Sampath, A.N.L. Japahuge and R.S. Jayakody
Department of Chemistry, University of Sri Jayewardenepura, Sri Lanka
ABSTRACT
Catalytic antibodies are immunoglobulin proteins that have catalytic activity similar to enzymes.
However, unlike enzymes abenzymes are capable of accepting a wide variety of substrates. Aldolase
catalytic antibody 38C2 catalyzes aldol and retro-aldol reactions via enamine mechanism. Main
features of abenzyme 38C2 are substrate orthogonality to natural aldolase and wide range of substrate
tolerance. Therefore, 38C2 has been used in selective prodrug activation and thereby, developed as a
tool for selective chemotherapy. However, its 3D atomic structure is not known yet. In this study, it
was attempted to construct the 3D atomic structure of humanized abenzyme 38C2 using its primary
amino acid sequence. Modeling was carried out by principles of Homology modeling. The3D atomic
structure of abenzyme 33f12 (PDB ID: 1AXT) was used as the template for the model construction
which shares 80% amino acid sequence identity. The accuracy of the constructed model was
evaluated using Ramachandran plot and the scoring function Qmean. Further, Dynamic stability was
evaluated using molecular dynamic simulations. Binding of natural substrate, acetone, to the novel
structure was studied using automated molecular docking. Docked complex’s dynamic stability was
observed from molecular dynamic simulations. Finally, the first step of the catalytic reaction has been
successfully carried out with IRC/QST3 calculations with DFT/B3LYP-6-31G level of theory in the
extracted catalytic residues in implicit water. This study concluded that the 3D structure of humanized
abenzyme 38C2 has successfully constructed and validated. The novel structure can be used to
enhance its application in drug discovery.
Keywords: Abenzyme, Aldolase,Homology modelling
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 23
C2 [18]
iOMICS: NEW PARADIGM IN ALGAL BIOTECHNOLOGY
P.P. Jutur
International Centre for Genetic Engineering and Biotechnology, New Delhi, India
ABSTRACT
Decreasing fossil fuels and its impact on global warming has led to an increasing demand for its
replacement by sustainable renewable biofuels. Microalgae may offer a potential feedstock for
renewable biofuels capable of converting atmospheric CO2 to substantial biomass and valuable
biofuels, which is of great importance for the food and energy industries. Parachlorella kessleri, a
marine unicellular green alga belonging to class Trebouxiophyceae, accumulates large amounts of oil
i.e., lipids under nutrient-deprived (-N, -P, and -S) conditions. Understanding their genomic and
metabolic imprints is important for elucidating the physiological mechanisms of lipid accumulations
in this microalga subjected to nutrient deprivation. One of feed-backward approach using metabolic
and lipidomic profiles by gas chromatography-mass spectrometry (GC-MS) of P. kessleri under
nutrient starvation (-N, -P and -S) conditions shown relative quantities of more than 100 metabolites
were significantlyexpressed compared in all these three starvation conditions. Our results demonstrate
that in lipid metabolism, the quantities of neutral lipids increased significantly followed by the
decrease in other metabolites involved in photosynthesis, nitrogen assimilation etc. In conclusion, the
metabolomics and lipidomic profiles have identified a few common metabolites such as citric acid,
valine and trehalose to play a significant role in the over production of oil by this microalga subjected
to nutrient deprivation. Understanding the entire system through iOmics (integrated omics) profiling
will lead to identifying relevant metabolic pathways involved in the biosynthesis and degradation of
precursor molecules that may have the potential for biofuel production, aiming towards the vision of
tomorrow’s bioenergy needs.
Keywords: algae, biofuels, omics, synthetic biology
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 24
C3 [19]
WHETHER MANKIND HAS A CHANCE OF SUCCOUR FROM THE PLANT
KINGDOM IN NEURODEGENERATION? AN In-Silico INVESTIGATION
S. Thiruchittampalam and S. Weerasinghe
Department of Chemistry, University of Colombo, Sri Lanka
ABSTRACT
Neurodegenerative diseases, Alzheimer’s (AD), Parkinson’s (PD), prion and Huntington’s (HD)
diseases afflicting large number of the population worldwide have been consolidated aiming
advancement of therapy simultaneously due to the causal tie-ups such as oxidative stress, protein
misfolding and aggregation. Most drugs that have been tried against these disorders cannot stop the
progression of the diseases and the chronic usage of the drugs enervates sufferers and caregivers due
to the abrupt side effects. Since the critical involvement of accumulation of certain metal ions in
oxidative stress, protein misfolding and aggregation, chelation therapy could be urged as a sensible
therapeutic remedy for neurodegeneration. Therefore, suitable molecules for the diseases related metal
ions Fe(III), Cu(II), Zn(II) and Al(III) were identified, computationally, to be used as chelating agents
from the database (http://scienc.cmb.ac.lk/tools/slflora) of ~200 molecules of native plants and
endemic plants of Sri Lanka, envisioning minimum side effects. The stability of metal-ligand
complexes was determined by calculating free energies of binding from the free energy landscape
along the reaction coordinate resulted as the upshot of umbrella sampling molecular dynamics
simulations. The simulation findings spots therapeutic potential compounds from 12 plants for AD,
PD and prion disease. Moreover, there is no potential compound to assort for the HD.
Keywords: neurodegeneration, chelation therapy, in-silico, free energy
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 25
C4 [20]
PROVING THE EFFICACY OF MANGIFERIN AS A NEUROPROTECTIVE DRUG
USING DOCKING STUDIES
P. Singh, R. Samadarsi and D. Dutta
Department of Biotechnology, National Institute of Technology Durgapur, West Bengal,
India
ABSTRACT
Mangiferin, a bioactive Xanthonoid known for its anti-oxidizing& therapeutic nature, has been
involved in a variety of potential pharmacological activities. The aim of this work is to prove the
efficacy of mangiferin as a neuroprotective drug by docking it with β-amyloid precursorprotein which
causes Alzheimer’s. Alzheimer’s disease (AD) is a progressive neurodegenerative disease which is
characterized by progressive cognitive deterioration and behavioral changes along with the chronic
inflammation of neurons. Studies have shown that the deposition of amyloid-β (Aβ) peptide have
resulted in the inflammatory changes such as astrocytosis and microgliosis. Based on the quantitative
analysis of the molecular docking technique it was observed that mangiferin successfully docked
withamyloid-β (Aβ) peptide. The ligand binding affinity was high due to the presence of many
flexible docked conformers with the most stable conformer having a binding energy of -12.513
kcal/mol. The analysis illustratesthe use of a computational approach to assess the use of mangiferin
as a potential nutraceutical.
Keywords: Mangiferin, β-amyloidpeptide (Aβ),Alzheimer’s disease, AutoDock, Binding Energy
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 26
C5 [21]
IDENTIFICATION AND In-Sillico CHARACTERIZATION OF A NOVEL Hindiii
ISOSCHIZOMER FROM A Pseudomonas SPP
S.N.J. Pathirana1, M. Kajan1, D.A.S. Elvitigala1, G.H.C.M. Hettiarachchi1,
C.M. Nanayakkara2, R.G.S.C. Rajapakse3 and N.V. Chandrasekharan1
1Department of Chemistry, University of Colombo, Colombo 03, Sri Lanka
2Department of Plant Sciences, University of Colombo, Colombo 03, Sri Lanka 3Department
of Molecular Biology and Biotechnology, Faculty of Science, University of Peradeniya,
Peradeniya, Sri Lanka
ABSTRACT
Type II Restriction enzymes (REs) can be used to cleave double stranded DNA in a sequence specific
manner to produce fragments with desired ends. Herein, we report the first attempt to screen and
isolate REs from bacteria in Sri Lankan soil and water samples collected from different regions of the
country. Bacteria isolated from samples were grown in Luria-Bertani (LB) medium. Lysates were
prepared and aliquots incubated with lambda DNA, and gel electrophoresed. Distinct banding patterns
indicate the presence of REs. Nine isolates belonging to different genera (Lysinibacillus,
Acinetobacter, Pseudomonas and Brevibacillus) as confirmed by morphological and molecular
characterization using 16S rRNA were positive for the presence of restriction endonucleases. The
lysate of one isolate belonging to the genus Pseudomonas was found to produce a banding pattern
similar to that of lambda DNA cleaved with HindIII. The enzyme was partially purified and optimum
conditions for enzyme activity and its recognition sequence was determined. The whole genome of
the Pseudomonas sp was sequenced and the gene for the putative HindIII isoschizomer identified
together with genes coding for other restriction enzymes. The gene coding for the HindIII
isoschizomer was bioinformatically analyzed and its homology and evolutionary relationship to other
known isoschozomers of HindIII determined. Molecular docking studies were also carried out using
its DNA ligand, reinforcing its functionality. Work is underway to clone the gene for production of
the recombinant enzyme.
Keywords: Restriction Enzymes, HindIII, Isoschizomer Whole genome sequencing
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 27
POSTER
PRESENTATIONS
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 28
P1 [22]
DETERMINATION OF DESIRABLE ATTRIBUTES OF A MEMBER OF
Burkholderia cepacia COMPLEX AS A BIOLOGICAL CONTROL AGENT OF
PLANT PATHOGENIC FUNGI
V.N.D. Bulathsinhalage and D.M. De Costa
Department of Agricultural Biology, Faculty of Agriculture, University of Peradeniya, Sri
Lanka
ABSTRACT
The present study was conducted to determine some of the desirable attributes of a bacterium
belonging to Burkholderia cepacia complex as a potential biological control agent in integrated
management of postharvest diseases of fruits and vegetables. In vitro assay was done by dual culture
plate technique using five fungal pathogens causing economically-important postharvest diseases,
namely, Botryodiplodia spp., Fusarium spp., Pestalotia spp., Colletotrichum gloeosporioides and
Colletotrichum capsici. The antagonistic ability of the bacterium and the cell-free filtrate of the
bacterium against the fungal pathogens was determined under in vitro conditions at the ambient
temperature. Moreover, antagonistic ability of the bacterium against Botryodiplodia spp. at different
temperatures (27, 37, 47, 57, 67 and 77 oC) was also determined under in vitro conditions. The
member of the B. cepacia complex showed a 58%, 45%, 59%, 50% and 54% colony growth
inhibition of Botryodiplodia spp., Fusarium spp., Pestalotia spp., C. gloeosporioides and C. capsici,
respectively at the 6th day of incubation. The antagonistic ability of the bacterium retained till 47 oC
and gave the same percentage colony inhibition against Botryodiplodia spp. However, cell-free
filtrate, stored at ambient temperature did not show antagonism against the fungal pathogens used for
the study. The findings revealed the broad-spectrum ability of the bacterium to be used for the
management of postharvest fungal pathogens and the ability of integrating the bacterium with an
appropriate hot water treatment for the management of postharvest diseases.
Keywords: Postharvest diseases, Botryodiplodia spp., Antagonism, Cell-free filtrate, Dual plate
method
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 29
P2 [23]
MOLECULAR CHARACTERIZATION OF GENUS Puntius USING
MITOCHONDRIAL GENE MARKERS FROM RIVERS OF SOUTHERN WESTERN
GHATS, INDIA
A.A. Arunkumar
Department of Biotechnology, Karpagam Academy of Higher Education, Tamil Nadu, India
ABSTRACT
Freshwater ecosystems contain only about 0.01% of the Earth’s water and the system accounts about
45% of fish species known to date. Alteration in the micro and macro habitats in the system severely
affects the aquatic life especially fishes and also complicates the fish taxonomy. Among the different
kinds of fresh water fishes, the fishes grouped under the genus Puntius, belonging to Cypriniformes
family, have been considered as an important protein source of daily diets of human beings in south
India, in particular. The inter-genetic and intra-genetic relationships of the Puntius species have not
yet been well understood to date and the species are reported to exist in various water bodies of
peninsular India. In the present study,the genomic DNA was isolated and PCR amplification was
carried out using universal primers for partial mitochondrial CO1 and 16sRNA sequences of
approximately 650bp length. Based on the obtained sequence of phylogenetic tree was constructed
using the Neighbour-Joining method using by Molecular Evolutionary Genetics Analysis (MEGA).
The present study provides a clear indication of the patterns of CO1sequence divergence within and
among the species.Mitochondrial CO1and 6sRNA genes resolve the taxonomic ambiguities of
genus Puntius. The less intra-specific variation was observed than the intraspecific variation in
both CO1 and 16sRNA genes and the phylogenetic tree expresses all the species of Puntius from
Southern Western Ghats to originate from a common ancestral group (i.e. all the species of the
genus Puntius has similar molecular status by which they fall under a single phylogenetic clad)
further more supporting to the field of Taxonomy of fresh water fishes of India. The significances
of the study and timely measures needed to protect the species have also been concisely discussed.
Keywords: Puntius, Southern Western Ghats, CO1, 16sRNA, Phylogeny
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 30
P3 [24]
DEVELOPMENT OF MICROBIAL INOCULUM TO ENHANCE THE
DEGRADATION OF ORGANIC WASTE
J.K.A. De Silva1, G.A.U. Jayasekera1 and C.M. Nanayakkara1
1Department of Plant Sciences, University of Colombo, Sri Lanka
ABSTRACT
Organic waste has become a critical issue with rising population, urbanization and economic activities
in Sri Lanka. There are number of methods that are used for organic waste management majoring
organic composting. However, the main problem of this process is that it needs much time to degrade
naturally. Therefore, this study is focused on the development of microbial consortium to enhance the
degradation of organic waste. This study found out the effective method to reduce the time taken for
degradation of 5kg of organic waste within 30 days by using Pseudomonas sp., Bacillus sp. and
Aspergillus sp. which are isolated from the Kaduwela garbage site, Sri Lanka. These microorganisms
were selected based on their extracellular enzyme activity by an enzyme assay and identified by using
biochemical and morphological identification methods. There were two types of treatments that were
used as individual and combination of the above mentioned microorganisms in three different
concentrations such as Mcfarland 1, 3 and 5 of bacterial isolates and spore suspension of Aspergillus
sp. which equals to 3x108spores /ml, 9x108spores/ml and 1.2x109spores/ml. Each of these
concentrations was applied individually and 3 combined concentrations as low (T1), moderate (T2)
and high (T3) of above mentioned microorganisms. The efficiency of degradation was analyzed by
comparing C/N ratio, mean of degradation using One-way-Analysis of Variance (ANOVA) and
degradation percentage. According to the results, the highest concentration of combined consortium
(T3), showed the lowest C/N ratio as 15:1 and the highest mean of degradation as 66.67%. With
regard to the use of One-way ANOVA for combination, the mean value of control and T1 (P = 0.004),
control and T2 (P = 0.001), control and T3 (P = 0.000) results obtained revealed that there were
significantly different, confirming that highest concentration of combination of these three
microorganisms was the best consortium to degrade organic waste efficiently.
Keywords: Organic waste, microbial consortium, gravimetric analysis, C/N ratio
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 31
P4 [25]
ENHANCED PRODUCTION OF Bacillus thuringiensis SUBSPECIES israelensis
DELTA ENDOTOXIN BY SUPPLEMENTATION OF ROTTEN MANGO JUICE
AND FISH-AMINO ACID AS MEDIUM SUPPLEMENTS
C. Gopinathan
Bioprocess Laboratory, Department of Biotechnology, University of Calicut, Kerala, India
ABSTRACT
Mosquito borne diseases not only cause a loss of lives but also impose heavy health and economic
burdens. Extensive use of chemical insecticides for the control of malaria and other mosquito borne
diseases has led to the development of resistance in mosquitoes to these insecticides and hazards to
the environment. Biolarvicides of the strain Bacillus thuringiensis israelensis, serotype H-14 is highly
effective against mosquito larvae. Even though Bti products are efficient controls for mosquito and
black fly larvae, their use in developing countries is limited by their cost. Thus, there is a need to
reduce the overall production cost of Bti in order to make it competitive in the market. It depends on
many factors; however, the raw material cost is one of the most important criteria, which may
comprise >70% of the overall production cost. Fruit wastes are available in plenty and contains
mainly fructose as the carbon source, which is easily frementable and can susbstitute costly
susbstrates like glucose. Channelising huge quantities of rotten/waste mangoes which other wise are
discarded can substantially reduce production cost of Bti. Similarly fish -amino acid produced by
fermenting rotten fish and jaggerry/molassess has proved to be an excellent as a medium supplement,
especially to overproduce the much wanted delta endotoxin produced by Bti. India is one the counties
leading in fruit and vegetable production.It is also blessed with one of the longest coastline in the
world of approx. 7516.6 km. The total annual catch is around 4 million metric tonnes. In addition it is
second after brazil in sugar cane cultivation with annual yield of 3412 million metric tons. The
massive availability of fruit wastes (mangoes) and huge quantities of rotten /discarded fish , which are
freely available, all can be channelised for cost effective production of this value added product,
substantially lowering the media cost of Bti production when scale- up is attempted. Results show
biomass increase of upto 29% compared to control, when rottenmangoe juice was used as the main
carbon source. The toxicity improvements with fish -amino acid supplemented medium shows
considerable reduction in killing time of Aedes aegyptii larvae.
Keywords: Bacillus thuringiensis subspecies israelensis, rotten mango, fish amino acid, delta
endotoxin, agro wastes
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 32
P5 [26]
RHEOLOGICAL PROPERTIES AND ANTIOXIDANT ACTIVITY OF HIGH
PRESSURE PROCESSED CITRUS JUICES
S. Karlović, T. Bosiljkov, F. Dujmić, F. Troha, M. Škegro, M. Marelja, M. Brnčić and
D. Ježek
Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia
ABSTRACT
Since most bioactive compounds and some physical properties degrade during high-temperature
pasteurization, novel high pressure (HHP) food processing technology is investigated as a potentially
viable alternative. Changes in textural and rheological properties of citrus fruits (Citrus x clementina)
were analyzed in fresh juice (reference values), after conventional pasteurization, as well as after
high-pressure processing (200, 400 and 600 MPa during 4, 8 and 12 min at 25 oC). Shelf life and
antioxidant activity were also investigated. Based on obtained results and concluded statistical
analysis (ANOVA, Tukey) process parameters having p < 0.5 were chosen as adequate. The color of
pasteurized juices degraded significantly, while in HHP juices remains very close to reference values,
with E=1,65 during whole shelf life. Viscosity and density of HHP treated juices also remained
stable and within 3,2 % of fresh juices. There were no observed changes in pH values. A total
antioxidant capacity value was 369,61 mgL-1 in fresh juice, with less than 5 % loss observed at 400
MPa. Pressures over 400 MPa could be considered as excessive, and optimal process parameters were
400 MPa at 12 min. This optimized HHP process yields significantly better results than conventional
processing, and the product is in all aspects comparable to fresh juice during whole investigated shelf
life.
Research in this paper is part of Croatian Science Foundation research project HIGHPRESS-JUICE.
Keywords: high hydrostatic pressure, clementine juice, rheological properties, TOAC
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 33
P6 [27]
BENIGN FABRICATION OF COPPER NANOPARTICLES FROM FLOWER
EXTRACT OF Cassia auriculata L
G.P. Chandran 1, T. Priyadharshini1, C. Rajkuberan1, N. Sekar1 and D.S. Kumar2
1Dept of Biotechnology, FASH, Karpagam Academy of Higher Education, Tamil Nadu, India
2Department of Biochemistry, FASH, Karpagam Academy of Higher Education, Tamil Nadu,
India
ABSTRACT
The present study was aimed to synthesis copper nanoparticles (CuNPs) in a greener route using
aqueous flower extract of Cassia auriculata. Initially, synthesis of CuNPs was confirmed through
UV-Vis spectroscopy which shows the surface plasmonic resonance peak (SPR) at 276nm. FTIR
analysis provides clear evidence that protein fractions present in the flower extract act as reducing and
stabilizing bio-agents. Energy dispersive X-ray (EDAX) spectroscopy confirms the presence of
copper as a major constituent element. Electron microscopic studies such as Field emission scanning
electron microscopic (Fe-SEM) and Transmission electron microscope (TEM) reveals that
synthesized CuNPs are spherical in shape with the size range between 5 and 30 nm. Further, flower
aqueous extract and synthesized CuNPs were evaluated against different bacterial and fungal
pathogens and when compared to the flower extract, biosynthesized CuNPs exhibits a remarkable
antimicrobial activity. The inhibition zones were ranged from 10-15 mm. Likewise C. auriculata
synthesized CuNPs demonstrated cytotoxic activity against tested Colon cell lines (CaCo2) and Dog
kidney cell lines (MDCK). The IC- 50 value for the C. auriculata CuNPs against CaCo2 and MDCK
cell lines were found to be 40 μg/ml respectively. The output of this study clearly suggesting that
biosynthesized CuNPs using aqueous flower extract of C. auriculata can be used as promising
nanomaterial for therapeutic application in context with nano-drug formulation.
Keywords: Cassia auriculata; Copper, Antibacterial and toxicity
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 34
P7 [28]
BIOSYNTHESIS OF SILVER NANOPARTICLES USING LEAF EXTRACT OF
Zingiber officinale (GINGER), Allium cepa VAR. Aggregatum (SHALLOT), Capsicum
annuum (BIRD’S EYE CHILI) AND Curcuma longa (TURMERIC):
DETERMINATION AND COMPARISON OF ANTIOXIDANT, ANTIMICROBIAL
PROPERTIES WITH LEAVES WATER EXTRACTION
Y.D.B. Peramuna and M. Kandiah
BMS, School of Science, Sri Lanka
ABSTRACT
Most of the disease conditions occurs due to an imbalance between free radicals and antioxidant
capacity. Therefore, antioxidants intake by daily diet to maintain the homeostasis of free radicals and
antioxidants to minimize the risk of having disease conditions. This novel study focused the
determination of antioxidant and antimicrobial activities in four different plant leaves of Zingiber
officinale (Ginger), Allium cepa var. aggregatum (Shallot), Capsicum annuum (Bird’s eye chili) and
Curcuma longa (Turmeric) in Sri Lanka. The water extraction of each sample was used to determine
antioxidant capacity by Total Antioxidant Capacity (TAC) assay, Total Phenolic Content (TPC) assay
and Total Flavonoid Content (TFC) assay, ABTS radical scavenging activity, IC50 and ferric
reducing ability of plasma (FRAP) assay. Furthermore, leaves water extract samples were used to
determine antimicrobial activity on test organisms Escherichia coli (gram-negative) and
Staphylococcus aureus (gram-positive) by well diffusion technique. The formation of silver
nanoparticles from ginger, shallot, Bird’s eye chili and turmeric were confirmed by UV-
spectrophotometry spectrum which gave a peak absorbance values around 400 nm. The study was
further enhanced to compare antioxidant capacity and activity along with antimicrobial activity of
silver nanoparticles with leaves water extract. The statistical analysis of P < 0.05 (P = 0.031599) only
in TAC assay indicates a significant difference between leaves water extract and silver nanoparticles,
confirming highest total antioxidant capacity (741.818 g/dm3) to be present in silver nanoparticles.
The silver nanoparticles showed the highest antimicrobial activity on both Escherichia coli (2.65 cm)
and Staphylococcus aureus (2.10 cm).
Keywords: silver nanoparticles, Total Antioxidant Capacity, Total Phenolic Content, Total Flavonoid
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 35
P8 [29]
AN IRIDOID GLYCOSIDE FROM THE SRI LANKAN MEDICINAL PLANT
Wendlandia bicuspidata
S. Rajendran1,2, S. Mohotti2, E.D. de Silva2,U. Göransson1,S. Gunasekera1 and C.M.
Hettiarachchi2
1Division of Phamacognosy, Department of Medicinal Chemistry, Uppsala University,
Biomedical Centre, Sweden
2Department of Chemistry, Faculty of Science, University of Colombo, Colombo 03, Sri
Lanka
ABSTRACT
We are presently engaged in the screening of Sri Lankan medicinal plants belonging to the family
Rubiaceae for bioactive metabolites. Wendlandia bicuspidata Wight & Arn is a large tree found in the
hilly region of Sri Lanka, mostly in Central province that has medicinal applications in traditional
medicine. The presence of Scandoside methyl ester has been reported previously fromW. bicuspidata.
Now we report the isolation and identification of Ixoside from the aqueous leaf extract. The plant was
collected in the area Uda Peradeniya in central province and it was identified by the National
herbarium, Peradeniya. Air dried, powdered leaf material was sequentially extracted first with Hexane
then dichloromethane, Ethylacetate and finally with methanol. A high yield of crude compound was
obtained from themethanolic extract, showing antibacterial activity in the agar dick diffusion assay.
Methanolic crude extract was partitioned with water to obtain water dissolvable polar compounds.
The water extract was fractionated on preparative high performance liquid chromatography with
gradient elution using ACN/H2O. Fractions obtained from HPLC were further analyzed by mass
spectrometry to obtain mass profiles of the fractions. The fraction with the compound Ixoside was
further purified using analytical HPLC and characterized with MS and MSMS techniques. The
structure of the molecule was elucidated with NMR techniques C13, H1, COSY, HMBC and HSQC.
Ixoside was obtained as a white water soluble compound with Minimum Inhibitory Concentration
against Staphylococcus aureus at 100 µg/ml.
Keywords: Wendlandia bicuspidata, Iridoid glycoside, NMR
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 36
P9 [30]
THE APPLICATION OF SURFACTANTS IN VOLATILE ORGANIC COMPOUND
(VOC) REMOVAL
L. Haddad, Z. Shareefdeen and H. Ahmed
American University of Sharjah, Sharjah, UAE
ABSTRACT
Volatile organic compounds (VOCs) are currently being emitted in large quantities; thus stringent
regulations are imposed on industry to control emission of toxic VOC pollutants. This works
compares the advantages and disadvantages of VOC removal alternatives which are mainly divided
into two categories, destruction and recovery. For the destruction techniques, it explores thermal
oxidation, catalytic oxidation, and bio-filtration. As for the recovery techniques, it investigates
adsorption, condensation, membrane separation, and most importantly absorption. The main focus of
the research was to look into the application of absorption as a technique to reduce the concentration
of the VOCs (i.e. toluene) through exploring different surfactant solutions as absorbents. Different
types of surfactant solutions were looked into for optimum removal efficiencies, and for the
operational and design parameters which affect them. Finally, some environmental implications were
explored which include the hazards of both VOCs and surfactants, but most importantly the reuse and
regeneration of surfactant solutions.
Keywords: volatile organic compounds, surfactants, absorption, toluene
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 37
P10 [31]
THE STUDY OF GENETIC VARIABILITY IN RELATED TO EFFICIENCY OF
ZINC OPTAKE IN DIFFERENT GENOTYPES OF TRITICUM AESTIVUM L.
Abdollah Hassanzadeh Ghorttapeh1, Aziz Majidi1, Javad Mozafari 2, Abdohosin Zyaeian3
1Horticulture Crops Department, West Azarbaijan Agricultural and Natural Resources
Research and Education Center, Agricultural Research, Education and Extinction
Organization, (AREEO), Urmia, Iran.
2Agricultural Research, Education and Extinction Organization, Tehran, Iran.
3Soil and Water Research Institute (SWRI). AREEO, Karaj, Iran.
ABSTRACT
Diversity in agro ecosystems can improve the sustainability of cropping systems in a context of low
external inputs and unpredictable climate change. Agricultural ecosystems are related to economical
and society condition widely, that there are in world. The major target of agricultural ecosystems
management is maximum nutrient flow and human service materials. Nutrient efficiency in wheat is
very complex. It includes nutrient acquisition efficiency and nutrient use efficiency. 26 winter wheat
genotypes were used to investigate the interactive effects between genotypes and the use efficiencies
of the Zn micronutrient by the grain. An experiment was carried out in Agricultural and Natural
Resources Research and Education, Center of West Azerbaijan, Genotypes were planted in complete
randomized block design with three replications. The traits such as plant height, biological and grain
yield, harvest index, length of spike, number of kernel per spike, thousand kernel weight, weight of
spike Zn uptake in grain were measured or calculated. It was revealed that biomass weight of whole
plot, plant height, number of kernel per spike, grain yield of main culms, biological yield, length of
spike and number of spikelet/spike, were positively and grain protein percentage, negatively correlate
with grain yield. Variance analysis showed that there were highly significant differences among traits.
The results obtained in this study indicate that nutrient use efficiency of the Zn varies widely within
wheat genotypes. Some genotypes were identified as being Zn use efficiency. These are considered
low-input genotypes. It appears that a special breeding programmer of crop cultivars for low Zn
nutrient and stress condition could be successful. Improved cultivar response to Zn nutrient will help
to reduce inputs and hence protect the environment.
Keywords: Wheat, Zn use efficiency, Genetic variability, low input
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
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VIRTUAL
PRESENTATIONS
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
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[32]
STOCK IDENTIFICATION AND SOME BIOLOGICAL ASPECTS OF FRIGATE
TUNA (Auxis thazard) IN SRI LANKAN WATERS
D.R. Herath1,2, U. Seneviratne1, H.A.C.C. Perera1 and G.H.C.M. Hettiarachchi2
1Marine Biological Resources Division, National Aquatic Resources Research and
Development Agency, Colombo 15, Sri Lanka
2Department of Chemistry, Faculty of Science, University of Colombo, Colombo 3, Sri Lanka
ABSTRACT
This study was carried out to determine the biology and the genetic stock structure of Frigate tuna,
Auxis thazard, an important neritic tuna fish species found in the Indian Ocean. A total of 354 frigate
tuna samples were collected from October 2015 to September 2017 from the coastal areas of Chilaw
and Kalpitiya in the North western province, Negombo and Beruwela in the Western province,
Dodanduwa, Galle, Weligama and Mirissa in the Southern province and Trincomalee in the Eastern
province of Sri Lanka. The length weight relationship for the collected samples was shown to be
W=0.828L3.433, which shows that the species has a healthy growth pattern. The analysis of the
stomach contents showed that they are nonselective feeders, feeding on diverse prey items such as
small fish species (herrings, sardines), shrimps, cephalopods and stomatopod larvae. The fecundity of
female A. thazard was shown to be 48,056 to 267,000 eggs. The GSI for female A. thazard fish
peaked during the period May to July indicating that their spawning season is during this period. The
stock structure was analyzed by studying the sequences of the mitochondrial COI region and the
mitochondrial DNA D-loop region. The phylogenetic analysis carried out using 21 sequences of
mitochondrial COI region and 23 sequences of mitochondrial control region, showed the fish of the
different regions studied being clustered together for both molecular markers. Therefore, when
management plans are being developed for A.thazard, the stocks of the different coastal areas could
be considered as belonging to one unit.
Keywords: Frigate tuna, Auxis thazard, stock identification, feeding, fecundity
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
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[33]
PRODUCTION OF PROTEIN HYDROLYSATES FROM FISH SKIN FOR DAIRY
PRODUCTS
E.I. Titov, G.V. Semenov, I.I. Ionova, I.S. Krasnova and N.S. Kozlov
Department “Technology and Biotechnology Meat, Fish and Dairy Products”, Moscow State
University of Food Production, Russia
ABSTRACT
The aim of this research was study the fish protein hydrolysates (FPH) from Salmon (Salmo salar)
skin prepared by different methods. The four methods: enzymatic hydrolysis, acid hydrolysis,
chemical (with acids or alkali) and enzymatic hydrolysis with preserving agents upon the heating
proposed to produce fish protein hydrolysates (FPH). All methods modified and adapted to optimize
researches. All hydrolysates were vacuum freeze-dried and collagen content was determined in them.
The results showed that FPH prepared using enzymatic hydrolysis with preserving agents upon the
heating had greater amount of protein than FPH prepared using other methods. The initial fish odour
and off-flavours generated in fish hydrolysate were masked by chitosan solution, citric acid
preparation and sesame seeds. The sensory analysis of the finish product allowed choosing the most
rational content of components. This FPH may potentially serve as a good source of protein. It could
be as an emulsifier and as a foaming agent in dairy products.
Keywords: fish protein hydrolysates, hydrolysis, fish odour, citric acid preparation, sesame seeds,
chitosan
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
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[34]
INFLUENCE THE PARAMETERS OF FREEZE DRYING ON ANTIOXIDANT
ACTIVITY OF YOGHURTS WITH DIFFERENT TROPICAL FRUITS
G.V. Semenov, I.I. Ionova, Z.V. Volokitina and I.S. Krasnova
Department “Technology and Biotechnology Meat, Fish and Dairy Products”, Moscow State
University of Food Production, Russia
ABSTRACT
Main patterns of interrelationship between process parameters and the preservation of dry
components in final product were determined in this article. Tropical fruits (jackfruit, melon, and
banana) were frozen in pieces. The process of freeze drying of the fruit raw material was at the
temperature of phase converting ice-to-vapour (from 100C) below zero to 350C below zero) at an
appropriate vacuum level. At the secondary drying the temperature was always the same, equal to 38-
40 °C. The final moisture content of the dried samples was 1.7-2.0%. Freeze drying fruits were easily
breakable and converted into powder. Each sample of dry fruit’s powder was added to the yogurts
during the fermentation. The total antioxidant activity was measured in the finished products by
coulometric titration with electrogenerated bromine using an EKSPERT 006 coulometric analyzer.
Researches showed that freeze drying at increasingly lower primary drying temperatures leads to an
increase the content of antioxidants.
Keywords: vacuum freeze drying, tropical fruits, yogurt, antioxidant activity, coulometric titration
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
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[35]
INFLUENCE OF HIGH HYDROSTATIC PRESSURE ON PHYSICAL
PROPERTIES OF APPLE JUICE
T. Bosiljkov, K. Klas, S. Karlović, F. Dujmić, M. Škegro, M. Marelja, M. Brnčić and
D. Ježek
Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia
ABSTRACT
Innovative and eco – friendly technologies are widely used in food processing with minimum
modification on the sensorial and organoleptic characteristics. The main goal of this research was an
analysis of the influence of the high hydrostatic pressure on physical properties of apple juice. The
influence of high hydrostatic pressure on absorbance, density, and particle size distribution was
observed under following conditions: Pressure (p = 200, 400, 600 MPa); Time of treatment (t = 5, 10,
20, 30 minutes). The treatment was conducted on apple juice obtained with filter diameter 0,3 and 0,5
mm. Statistically significant influence of process parameters is expressed over p – values. ANOVA
factors having p<0,05 were taken into consideration. The stability of apple juice can be observed by
measuring the absorbance and expressed by JSI [h] (Juice Stability Index). Apple juice made with
filter diameter 0,5 mm has greater stability where values of the JSI are in the range 47 – 224 h with
optimal process parameters by 400 MPa and 20 minutes (232 h); 600 MPa and 10 minutes (244 h).
Particle size distribution depending on relative frequency indicates stability in the obtained interval
between 0,1 – 3 µm. High hydrostatic pressure and time of treatment have no significant influence on
density value.
Keywords: high hydrostatic pressure, apple juice, juice stability index, particle size distribution
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
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[36]
DEGRADATION OF 2,4,6 TNT BY NATIVE MICROORGANISMS ISOLATED
FROM TNT CONTAMINATED SOIL
M. Devasena and A. Thenmozhi
Sri Krishna College of Technology, Coimbatore, Tamilnadu, India
ABSTRACT
Soil contamination from toxic explosive is a major environmental concern at military shooting ranges,
storage sites and disposal areas in many parts of the world.2,4,6-Trinitrotoluene (TNT) is the most
widely used secondary explosive material and it constitutes a special contamination problem.TNT and
its derivatives are toxic and recalcitrant and are biodegraded relatively slowly in the environment. The
present work aims to isolate, characterize and enrich the microbial population in TNT contaminated
samples collected from different sites. The feasibility of using Bacillus subtilis, Pseudomonas
aeruginosa, Staphylococcus aureus and Serratia marcescens for TNT degradation was analysed
under various experimental conditions. Change in soil properties and change in TNT concentration
were made to understand the tolerance limit of the native microorganisms. Biodegradable ability of
the bacterial population was then performed with different concentrations of TNT. The degradation
potential, metabolites and the pathway for degradation were then elucidated and confirmed by HPLC
analysis. The native microbes were able to degrade TNT and may be possessing a huge potential for
enhanced degradation of contaminants.
Keywords: TNT, aerobic, anaerobic, Bacillus, subsurface
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
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[37]
MULTI FEATURES CLUSTERING APPROACH TO DETERMINE
PARKINSONIAN DISEASE UPON GAIT MOVEMENT ARTIFACTS
S. Anujan and M. S. Karunarathne
Sabaragamuwa University of Sri Lanka
ABSTRACT
Parkinson’s disease is a neurodegenerative brain disorder that progresses slowly in most elderly
people. Symptoms can take years to develop, and most people live for many years with the disease.
The symptoms caused by Parkinson’s include an ongoing loss of motor control as well as a wide
range of non-motor symptoms. Since the victims of Parkinsonian disease are senior citizens who often
have difficulties in motor ability, it is challenging to identify the symptoms of Parkinsonian disease at
the initial stages. Therefore, we have attempted to distinguish eight Parkinson’s patients from eight
healthy subjects using their gait information. Our analysis is based on cumulative vertical ground
reaction forces collected from 16 Sensors located under the left foot and right foot of 16 Parkinsonian
and non-Parkinsonian subjects during usual walking. We have applied time varied entropy techniques
(Sample Entropy and Approximate Entropy) and fast Fourier transform over the sensor measurements
sampled at 100 Hz. We calculated the mean values of sample entropy and approximate entropy
relevant to gait movement for each left leg and right leg of each subject. The fourth dominant
frequency were calculated for each gait movements conducting fast Fourier transform over sensor
measurements. The calculated Sample Entropy, Approximate Entropy and fourth dominant frequency
were ranged respectively (-4.4 to -5.2), (0.02 - 0.06) and (10 Hz- 65 Hz). The three features (Sample
Entropy, Approximate Entropy and Fourthly dominant frequency) of the movements were plotted in
three dimensional sphere. Indeed, the Parkinsonian patients and healthy subjects could be grouped
handily in to two clusters with 100% accuracy.
Keywords: Parkinson’s disease, Fast Fourier Transform, Sample Entropy, Approximate Entropy, Gait
Analysis
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
Page | 45
[38]
ULTRASONIC - ASSISTED EXTRACTION OF BIOACTIVE COMPOUNDS FROM
WASTE IN PRODUCTION OF RED WINE
F. Dujmić, S. Karlović, T. Bosiljkov, F. Troha, M. Škegro, M. Marelja, D. Ježek, R. Vidrih
and M. Brnčić
Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia
ABSTRACT
Grapes as the fruit are produced more than 60 million tons per year and are processed into wine and
other grape products, and consequently are source of large quantities of organic waste. Wine lees are
one of the by-products of the wine production. Wine lees mainly refer to remaining sediment in
vessels containing wine after fermentation and during the maturing of the wine. grape skins and seeds
contain large quantities of bioactive compounds, such as anthocyanins. These compounds can be
extracted using conventional techniques such as maceration, or novel nonthermal extraction
technologies that include high hydrostatic pressure, high-intensity ultrasound, as well as pulsed
electric fields. In this research, are investigated influence of ultrasonic parameters - on yield of
extracted polyphenolic compounds from wine lees of Blatina cultivar. Effect of process parameters
such as extraction time (five different times from 60 s to 2700 s), amplitude (five different values
from 20 % to 100 %) of 400 W ultrasonic processor and probe diameter (7 mm, and 40 mm) at
starting extraction temperature at 25 °C on quantity and composition of extracted bioactive
compounds was investigated. Optimization of ultrasound-assisted extraction of anthocyanins from
Blatina cultivar wine lees is carried out using Design Expert software. Results show increases
extraction yield from 20 % to 70 %. Optimal process time of ultrasonic extraction was up to 80 times
shorter compared to conventional extraction. Calculation of optimal UAE process parameters based
on the obtained data shows that the best results occur using 90 % of maximal amplitude, during 1800
s using 40 mm ultrasonic probe.
Keywords: Ultrasound, wine lees, extraction parameters, HPLC, antioxidants
The 3rd International Conference on Bioscience and Biotechnology (BioTech 2018)
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