bloodborne pathogens in the unc-ch laboratory setting

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Bloodborne Pathogens (BBP) Exposure Control Program For Laboratory Workers At UNC Chapel Hill In accordance with Occupational Safety and Health Administration (OSHA) Bloodborne Pathogen Standard 29 CFR 1910.1030 1

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Page 1: Bloodborne Pathogens in the UNC-CH Laboratory Setting

Bloodborne Pathogens (BBP)Exposure Control Program

For

Laboratory Workers

At UNC Chapel Hill

In accordance with

Occupational Safety and Health

Administration (OSHA)

Bloodborne Pathogen Standard29 CFR 1910.1030

1

Page 2: Bloodborne Pathogens in the UNC-CH Laboratory Setting

Questions?? Call 962-5507 between 8 a.m. & 5 p.m.

2

In case of emergency…

Knowing the right steps to take after an exposure incident is critical to reducing the likelihood of acquiring a bloodborne pathogen. Immediately after any exposure incident:

• Wash the exposed area with soap and water; if the exposure involves the eyes, you should flush with tap water

• Immediately report the incident to your supervisor and/or immediately call the University Employee Occupational Health (UEOHC) at 6-9119.

Emergency Contact Numbers

UEOHC Clinic: 966-9119

Emergency: 9-911

Questions regarding this training: 962-5507

Page 3: Bloodborne Pathogens in the UNC-CH Laboratory Setting

Questions?? Call 962-5507 between 8 a.m. & 5 p.m.

3

INTRODUCTION

Page 4: Bloodborne Pathogens in the UNC-CH Laboratory Setting

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4

BBP Training: Why should we do it?

1. It’s the law

2. It’s UNC’s responsibility

3. Knowing this information can protect your health and the health of others

Page 5: Bloodborne Pathogens in the UNC-CH Laboratory Setting

Questions?? Call 962-5507 between 8 a.m. & 5 p.m.

5

BBP Training: Why should we do it?

1. It’s the law2. It’s UNC’s responsibility 3. Knowing this information can protect your

health and the health of others

• On March 6, 1992, OSHA created the Bloodborne Pathogen (BBP) Standard to make sure all employers strive to keep their workers safe from bloodborne pathogens.

• OSHA considers the Senior Scientist or Principal Investigator (PI) of each laboratory to be an employer. You work under the guidance of your PI for some benefit; be it knowledge, experience, or monetary compensation.

• Most hazardous laboratory tasks are covered under the OSHA Laboratory Standard and, at UNC, these are tracked in your lab’s Laboratory Safety Plan.

• Some laboratory tasks are more likely to come into contact with bloodborne pathogens.

• OSHA regulates potential bloodborne pathogen exposure separately from the Laboratory Standard. The OSHA Standard requires annual training and a vaccination or declination for Hepatitis B virus.

The complete OSHA BloodbornePathogens Standard is available at the OSHA website.

Page 6: Bloodborne Pathogens in the UNC-CH Laboratory Setting

Questions?? Call 962-5507 between 8 a.m. & 5 p.m.

6

BBP Training: Why should we do it?

1. It’s the law2. It’s UNC’s responsibility 3. Knowing this information can protect your health and

the health of others

• Employers demonstrate to OSHA that they are following the Bloodborne Pathogens Standard by implementing an Exposure Control Plan.

• At UNC, every Principal Investigator adopts the UNC Laboratory Exposure Control Plan.

• The UNC Laboratory Exposure Control Plan lists the steps UNC is taking to keep lab workers safe. It is the plan to control every lab worker’s exposure to bloodborne pathogens.

• Annual training on the Exposure Control Plan is one requirement of the OSHA BBP Standard. The other requirement is a vaccination/declination for Hepatitis B virus.

• As a lab worker, this training fulfills your training requirement.

• The complete UNC Laboratory Exposure Control Plan is available on the EHS website.

Page 7: Bloodborne Pathogens in the UNC-CH Laboratory Setting

Questions?? Call 962-5507 between 8 a.m. & 5 p.m.

7

BBP training: Why should we do it?

1. It’s the law2. It’s UNC’s responsibility 3. Knowing this information can protect your

health and the health of others

• For lab workers, the potential for exposure to some bloodborne pathogens may exist during certain procedures, using particular devices, or when encountering spilled human material such as cell lines, blood , or body fluids, or improperly handling waste from your laboratory.

• Bloodborne pathogens and material potentially containing bloodborne pathogens are handled at Biosafety Level 2 (BSL-2).

• The OSHA Standard requires the use of the biohazard symbol to allow for communication of potential biological hazards.

Page 8: Bloodborne Pathogens in the UNC-CH Laboratory Setting

Questions?? Call 962-5507 between 8 a.m. & 5 p.m.

8

EXPOSURE DETERMINATION IN THE UNC LABORATORY

Page 9: Bloodborne Pathogens in the UNC-CH Laboratory Setting

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9

Exposure Determination

Each Principal Investigator is responsible for ensuring the completion of the exposure determination for each employee who works with blood and/or Other Potentially Infectious Material (OPIM) including human cell lines.

This should be completed upon orientation and “no later than 10 days after the date of employment” (OSHA). The exposure determination is documented via:

• The Laboratory Safety Plan Schedule A (updated annually);

• The employee Lab Worker Registration Form; and

• Followed-up during laboratory safety audits conducted by UNC EHS.

This determination must made without regard to use of PPE.

Page 10: Bloodborne Pathogens in the UNC-CH Laboratory Setting

Questions?? Call 962-5507 between 8 a.m. & 5 p.m.

10

I work in a lab, how do I determine“Occupational Exposure”?

The OSHA definition of Occupational Exposure is

Reasonably anticipated skin, eye, mucous membrane or parenteral contact with blood or other potentially infectious materials that may result from the performance of an employee’s job duties.

…for definitions f blood or OPIM other potentially infectious materials), read on

Parenteral contact is:

piercing mucous membranes or the skin by needlesticks, human bites, cuts and abrasions.

Page 11: Bloodborne Pathogens in the UNC-CH Laboratory Setting

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11

For the purpose of making an Exposure Determination, OSHA defines blood as:

Blood:

Human blood, human blood

components, products made from human blood

…so if you work with this material in a lab setting, you need to be enrolled in the Bloodborne Pathogens program.

Page 12: Bloodborne Pathogens in the UNC-CH Laboratory Setting

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12

Other Potentially Infectious Material

This includes:

• Certain human body fluids

• Any unfixed tissue or organ except intact skin from human (living or dead)

• Human cell lines

For the purpose of making an

Exposure Determination,

OSHA defines OPIM as:

Page 13: Bloodborne Pathogens in the UNC-CH Laboratory Setting

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13

OPIM: Other Potentially Infectious Material

Certain human body fluids include:

• Semen

• Vaginal secretions

• Cerebrospinal fluid

• Synovial fluid

• Pleural fluid

• Pericardial fluid

• Peritoneal fluid

• Amniotic fluid

• Saliva in dental procedures

• Any body fluid visibly contaminated with blood

• All body fluids when it is difficult to differentiate between body fluids

Page 14: Bloodborne Pathogens in the UNC-CH Laboratory Setting

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14

• OSHA's position has always been that workers handling human cell cultures (primary or characterized) fall under the Bloodborne Pathogen (BBP) Standard unless the cells have been tested and proven to be free of bloodborne pathogens.

• Because it is difficult to have cells tested for all bloodborne pathogens and ensure that they are never contaminated with bloodborne pathogens during research, UNC has taken the position that work with ALL human cell lines (including established lines) fall under the requirements of the BBP standard.

A HeLa Cell Line named after Heinratta Lacks, one of the earliest human cell lines

Human Cell Lines

•OSHA’s interpretation of the BBP Standard that includes human cell lines is available here: Applicability of 1910.1030 to established human cell lines (06/21/1994)

Page 15: Bloodborne Pathogens in the UNC-CH Laboratory Setting

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15

GENERAL RISKS AND TRANSMISSION

Page 16: Bloodborne Pathogens in the UNC-CH Laboratory Setting

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16

What are Bloodborne Pathogens?

OSHA defines bloodborne pathogens as:

Pathogenic microorganisms that are present in human blood that can cause disease in humans.

The risks and transmission of the following bloodborne pathogens will be discussed in this training:

• hepatitis B virus (HBV)• hepatitis C virus• human immunodeficiency virus (HIV)

Many diseases are linked to bloodborne pathogens, but few bloodborne pathogens are frequently responsible for infections in the workplace.

Page 17: Bloodborne Pathogens in the UNC-CH Laboratory Setting

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17

Other Bloodborne Pathogens Include:

• Non-A, non-B hepatitis

• Treponema pallidum (syphilis)

• Plasmodium spp. (malaria)

• Brucella spp.

• Leptospira interrogans

• Arboviruses

• Borreliae

• Creutzfeldt-Jakob disease agent

• Hemorrhagic fever viruses

Page 18: Bloodborne Pathogens in the UNC-CH Laboratory Setting

Occupational transmission of HIV among health care workers.

Questions?? Call 962-5507 between 8 a.m. & 5 p.m.

18

(Source: CDC 2003)

As of December 2001, occupational exposure to HIV has resulted in 57 documented cases of HIV among healthcare personnel in the United States.

Page 19: Bloodborne Pathogens in the UNC-CH Laboratory Setting

Questions?? Call 962-5507 between 8 a.m. & 5 p.m.

19

Laboratory Acquired HIV

Two workers in different laboratories producing large quantities of highlyconcentrated HIV were documented to have laboratory-acquired HIVinfections.

One worker's infection was presumed to have been caused by "undetected skin contact with virus culture supernatant". The most probable cause for this infection was inapparent parenteral exposure. Gloves of questionable integrity, skin cuts and abrasions, and one episode of a dermatitis-like condition represented portals for possible exposure and routes of infection.

The other worker's infection followed "an injury with a potentially contaminated needle". The most probable cause for the second worker's infection was parenteral inoculation. This worker recalled incurring an injury with a blunt cannula approximately 6 months before the first seropositive sample.

Source: MMWR 37(S-4);19-22, Publication date: 04/01/1988

Page 20: Bloodborne Pathogens in the UNC-CH Laboratory Setting

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20

Clinical Manifestations of HIV Infection

The clinical symptoms of HIV infection ranges from those who have no symptoms to those with severe immunodeficiency or Acquired Immune Deficiency Syndrome (AIDS).

Initial infection can be followed by an acute flu-like illness with non-specific symptoms such as fever, swollen lymph nodes, rash, malaise, sore throat, and headache.

Other symptoms can include anorexia, chronic diarrhea, weight loss, and fatigue.

Opportunistic infections and malignant diseases without a known cause for immune deficiency can also be indicative.

Page 21: Bloodborne Pathogens in the UNC-CH Laboratory Setting

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21

HIV Infection

Without treatment, the natural history of HIV infection can vary

considerably from person to person. The risk for disease progression increases with the duration of infection. Approximately 20-25% of HIV-infected adults will develop AIDS within 6 years after infection and 50% within 10 years. When an HIV-infected person develops certain diseases or conditions, they are then classified as having AIDS. Three of the most common clinical conditions are P. carinnii pneumonia, HIV wasting

syndrome and candidiasis of the esophagus.

Wasting Syndrome

Page 22: Bloodborne Pathogens in the UNC-CH Laboratory Setting

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22

Hepatitis B in the U.S.

Annually, there are 10,000 new cases of Hepatitis B virus (HBV) reported in the U.S., with an estimated one to two million carriers of HBV. The prevalence of serologic markers for present or past Hepatitis B infection is approximately 5%.

Studies have found a prevalence of Hepatitis B to be approximately 2% in the general U.S. population.

(Source: www.cdc.gov)

Viral hepatitis infects the liver

Page 23: Bloodborne Pathogens in the UNC-CH Laboratory Setting

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23

Estimated number of occupational Hepatitis B infections among U.S. health care workers, 1983–1999

In the mid-1980s, health care facilities began adopting “universal precautions” against exposure to body fluids. These were followed in 1992 with the OSHA BBP Standard.

Page 24: Bloodborne Pathogens in the UNC-CH Laboratory Setting

Questions?? Call 962-5507 between 8 a.m. & 5 p.m.

24

Clinical Manifestations of Hepatitis B Virus Infection

1. 67% to 75% of infected persons have no symptoms or mild flu-like illness

2. 25% to 33% have a more severe clinical course:

• Extreme fatigue, anorexia, nausea, abdominal and joint pain, rash, fever

• Symptoms last for 3-10 days. This is followed by onset of jaundice or dark urine.

• Severe acute liver failure with Hepatic encephalopathy (brain dysfunction directly due to liver dysfunction) can occur within 8 weeks of the onset of jaundice.

Hepatitis B virus

Page 25: Bloodborne Pathogens in the UNC-CH Laboratory Setting

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25

Hepatitis B VaccinationEmployees who have reasonably anticipated exposure to blood or OPIM will

be offered the Hepatitis B vaccination free of charge through the University Employee Occupational Health Clinic (UEOHC) located at 145 N. Medical Drive.

This vaccination must be made available within 10 working days of initial assignment or the Hepatitis B

declination form must be signed.

Page 26: Bloodborne Pathogens in the UNC-CH Laboratory Setting

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26

General Hepatitis B Vaccination Information

Recombinant vaccines for HBV were licensed in the US in 1986. Given as a series of three injections, the vaccine produces a high antibody titer in over 90% of the population.

The vaccine is safe and well tolerated by recipients. In about 1 out of 4 adults, soreness where the shot was given, lasting a day or two was noted and mild to moderate fever was noted in 1 out of 100 adults.

Employees with life threatening allergies to baker’s yeast should not receive the Hepatitis B vaccination.

For more information about the Hepatitis B vaccination series, view the CDC fact sheet.

Page 27: Bloodborne Pathogens in the UNC-CH Laboratory Setting

Hepatitis B Vaccination Requirement

Do you still need to schedule or decline the Hepatitis B vaccine? This requirement needs to be met within 10 working days after your exposure determination is made.

The vaccine is offered by UNC at no cost to you.

To obtain the Hepatitis B vaccination, complete these steps:

1. Fill out the Hepatitis B vaccination consent form available here.

2. If you don't have a medical record number, call 966-2555 to get one.

3. Schedule your appointment with the University Employee Occupational Health Clinic at 966-9119.

To Decline the Hepatitis B vaccination: Complete this form and follow the directions on the form to submit it to the University Employee Occupational Health Clinic.

Questions?? Call 962-5507 between 8 a.m. & 5 p.m.

27

Go on, do it now.

Page 28: Bloodborne Pathogens in the UNC-CH Laboratory Setting

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28

Clinical Manifestations of Hepatitis C Virus Infection

Hepatitis C is the most common chronic bloodborne infection in the US and is the leading indication for liver transplantation.• 25-30% of infections are asymptomatic• 55-85% of infected persons develop long-term infection • 70% of infected persons develop chronic liver disease • 5-20% of infected persons develop cirrhosis over a period of 20 to

30 years • 1-5% of infected persons die from the consequences of long term

infection (liver cancer or cirrhosis) Symptoms include jaundice, loss of appetite, fatigue, intermittent nausea, abdominal pain and vomiting.

There is no vaccine for Hepatitis C.

Page 29: Bloodborne Pathogens in the UNC-CH Laboratory Setting

Questions?? Call 962-5507 between 8 a.m. & 5 p.m.

29

Transmission

In the lab setting, bloodborne pathogens are transmitted by:• Percutaneous exposure such as needlesticks, cuts with sharp objects

contaminated with blood/OPIM

• Direct contact of blood/OPIM with non-intact skin.

• Mucous membrane exposure such as splashes of blood/OPIM to the eyes, nose and mouth.

HIV and HBV can also be transmitted from mother to unborn child. If you are pregnant or intend to become pregnant, please contact EHS at 962-

5507 for information about the UNC conceptus protection policy.

Page 30: Bloodborne Pathogens in the UNC-CH Laboratory Setting

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30

Health Care Worker BBP Exposures

82%

14%

3%1%

Percutaneous

Contact with mucous membranes

Exposure of broken or abraded skin

Human bites

Page 31: Bloodborne Pathogens in the UNC-CH Laboratory Setting

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31

Percutaneous Injuries

Up to 800,000 percutaneous injuries may occur annually among all U.S. health care workers (both hospital-based workers and those in other health care settings).

After percutaneous injury with a contaminated sharp instrument, the average risk of infection is 0.3% for HIV and ranges from 6% to 30% for hepatitis B.

Distribution of 10,378 reported percutaneous injuries among hospital workers by medical device associated with the injury, 1995-2000 (Source: CDC 2002)

Page 32: Bloodborne Pathogens in the UNC-CH Laboratory Setting

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32

Laboratory Associated InfectionsA study conducted by Pike in 1979 found that only a small proportion of all lab acquired infections (not just BBP) were associated with an identifiable accident. This study was updated in 1999 (Harding and Byers) and they also found a small percentage of lab acquired infections were linked to a specific accident.

Accidents Associated with Lab Acquired

Infections (Pike 1979)

25%

16%

26%

13%

14%6%

Needle and syringe

Broken glass, other sharp

Spills, sprays

Aspiration through pipette

Animal bite or scratch

Other

Page 33: Bloodborne Pathogens in the UNC-CH Laboratory Setting

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33

PREVENTING INCIDENTS AT UNC

Page 34: Bloodborne Pathogens in the UNC-CH Laboratory Setting

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34

Now that we’ve discussed how to respond in the event of a potential exposure, let’s look at how to protect ourselves and others from exposure.

Make sure you know what’s expected to keep your lab safe according to the OSHA BBP Standard:

(1) To prevent exposures to lab workers and

(2) To protect infrequent visitors to the lab (such as UNC Facilities Services workers, etc.) against exposures

PREVENTING INCIDENTS AT UNC

Page 35: Bloodborne Pathogens in the UNC-CH Laboratory Setting

Universal Precautions• Universal Precautions is an approach to infection control to prevent contact

with blood or "other potentially infectious materials". These laboratorianspractice Universal Precautions by safely handling all human blood and OPIM research material as if they are infected with HIV, HBV, and other bloodborne pathogens.

• Universal Precautions is a simple approach toward protecting yourself in the laboratory environment.

• With Universal Precautions, you wear PPE when you anticipate contact with any and all human blood, body fluids, or OPIM.

Questions?? Call 962-5507 between 8 a.m. & 5 p.m.

35

Page 36: Bloodborne Pathogens in the UNC-CH Laboratory Setting

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36

Biosafety Level 2 (BSL-2)• To implement proper Universal Precautions

under the OSHA BBP Standard at UNC, the lab worker is required to have basic knowledge of BSL-2 practices as described by the CDC/NIH.

• Unlike the Bloodborne Pathogens training that is required annually, the BSL-2 training is a one-time requirement. BSL-2 training is available online.

• Training history can be reviewed online to determine if you’ve met the requirement.

Page 37: Bloodborne Pathogens in the UNC-CH Laboratory Setting

Bloodborne Pathogens Spills

Spills may occur when containers of blood or other potentially infectious materials (OPIM) are dropped in the clinic or laboratory.

Employees designated to participate in emergency and decontamination procedures are exposed to blood or OPIM; they are to be thoroughly familiar with proper cleaning and decontamination procedures so that the contamination is contained and exposure to other people is minimized.

37Questions?? Call 962-5507 between 8 a.m.

& 5 p.m.

Page 38: Bloodborne Pathogens in the UNC-CH Laboratory Setting

Response to a BBP Spill

1. Evacuate laboratory and alert people in the immediate spill area

2. Remove any contaminated clothing and wash exposed skin with disinfectant

3. Notify PI and EHS (962-5507)

4. Wait 30 minutes before reentering. During this time the aerosol will be removed from the laboratory by the exhaust air ventilation system.

5. Re-enter wearing PPE --Lab coat with long sleeves, disposable gloves and shoe covers, eye/face protection

6. Clean-up

7. Use mechanical means to provide barrier from broken glass

8. Cover with paper towels or other absorbent materials

9. Pour appropriate disinfectant (i.e. diluted bleach) around and in spill area. Avoid splashing.

10. Allow 20 minutes contact time.

11. Use paper towels to wipe up spill working from the edges into the center

12. Place materials used to clean up spill, including PPE, in orange biohazard bag

13. Wash hands

14. Tape “X” on autoclave symbol with autoclave tape

15. Autoclave materials

16. Wash hands

Questions?? Call 962-5507 between 8 a.m. & 5 p.m.

38

1:10 dilution of household bleach

20 minute contact time Disinfect

Page 39: Bloodborne Pathogens in the UNC-CH Laboratory Setting

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Remember Your Sharps Precautions During a Spill

Contaminated broken glassware is cleaned up by mechanical means (e.g.

tongs, forceps, pieces of cardboard).

Questions?? Call 962-5507 between 8 a.m. & 5 p.m.

Page 40: Bloodborne Pathogens in the UNC-CH Laboratory Setting

Biohazard Waste Disposal

North Carolina Medical Waste Rules describes biological waste as:

Blood and body fluids in individual containers in volumes greater than 20 ml

20 ml liquid ~ ½ shot glass

Questions?? Call 962-5507 between 8 a.m. & 5 p.m.

40

Page 41: Bloodborne Pathogens in the UNC-CH Laboratory Setting

Biohazard WasteResearchers are responsible for properly treating Biohazard waste and

sharps generated in their labs. Waste is to be treated according to procedures outlined in the UNC Biological Waste Disposal Policy.

Most researchers treat their waste in an autoclave. Autoclaving is the most dependable procedure for the destruction of all forms of germs including bloodbornepathogens.

For more information, Autoclave Usage and Safety training is available online.

Questions?? Call 962-5507 between 8 a.m. & 5 p.m.

41

Page 42: Bloodborne Pathogens in the UNC-CH Laboratory Setting

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42

Biohazard labels are used to alert others of the potential presence of biohazardous materials such as human blood, body fluids, and OPIM.

These labels are fluorescent orange or red/orange with contrasting letters and have the universal biohazard symbol.

Biohazard Labels

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At UNC, biohazard labels are used on:• Containers of biohazard waste• Refrigerators and freezers used to store human specimens• Containers used to store, transport and ship human specimens• Any equipment that could be potentially contaminated with human blood,

OPIM, and other material that could allow bloodborne pathogens to live or to grow.

Biohazard Labels

Questions?? Call 962-5507 between 8 a.m. & 5 p.m.

Page 44: Bloodborne Pathogens in the UNC-CH Laboratory Setting

Engineering and Work Practice Controls

To eliminate or minimize employee exposure The BBP Standard requires the implementation of engineering and work practice controls.

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44

Page 45: Bloodborne Pathogens in the UNC-CH Laboratory Setting

Engineering and Work Practice Controls

Engineering controls are equipment or devices used to control exposures and at UNC include sharps disposal containers, safer needle devices, and biological safety cabinets.

Work Practice Controls specifically reduce the likelihood of exposure by altering the manner in which a task is performed.

Questions?? Call 962-5507 between 8 a.m. & 5 p.m.

45

Page 46: Bloodborne Pathogens in the UNC-CH Laboratory Setting

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Disposal of Biohazard Waste: SHARPSBecause percutaneous exposure (through the skin) is a primary route of

transmission at BSL-2, extreme caution should be taken with contaminated

needles or sharp instruments.

Needles/contaminated sharps:

• Must never be bent, recapped or removed unless there is no alternative

• May only be recapped using a mechanical device or one handed technique

• Must never be sheared or broken

Page 47: Bloodborne Pathogens in the UNC-CH Laboratory Setting

Needles & Sharps Precautions

47

Contaminated sharps must be

placed in red, hard walled plastic

containers labeled with the

biohazard symbol as shown.

When the container is no more

than 2/3 full, place autoclave

indicator tape over the biohazard

symbol in an “X” pattern as

shown.

Containers must be placed in

biohazard bag prior to

autoclaving.

Page 48: Bloodborne Pathogens in the UNC-CH Laboratory Setting

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48

Safer Needle Devices

• When possible, safer needle devices should be used in the laboratory.

• Examples of safer devices, including interactive animations, can be found on the OSHA website at the Safer Needles Section.

Page 49: Bloodborne Pathogens in the UNC-CH Laboratory Setting

Questions?? Call 962-5507 between 8 a.m. & 5 p.m. 49

Biological Safety Cabinets

Biological Safety Cabinets are used in laboratories when conducting procedures with a potential for creating aerosols or splashes of blood/OPIM.

Page 50: Bloodborne Pathogens in the UNC-CH Laboratory Setting

Minimize Splashes. Your technique is important. Germs can spread more readily when we cause splashing, spraying, spattering, and airborne droplets of blood or other potentially infectious materials.

Work Practice Controls

50Questions?? Call 962-5507 between 8 a.m.

& 5 p.m.

Hand washing. Hands are to be washed immediately or as soon as feasible after removal of gloves or other personal protective equipment.

Do not use sinks in food preparation areas. If hand washing facilities are not immediately available use antiseptic hand cleanser and/or disposable wipes.

Page 51: Bloodborne Pathogens in the UNC-CH Laboratory Setting

Work Practice ControlsPrevent Ingestion. Eating, drinking,

smoking, applying cosmetics or lip balm, and handling contact lenses are prohibited during duties where there is reasonable likelihood of occupational exposure to blood or other potentially infectious material.

Storage of food and drink is prohibited in refrigerators, freezers, shelves, cabinets or on countertops or bench tops where blood or other potentially infectious materials are present.

Questions?? Call 962-5507 between 8 a.m. & 5 p.m.

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Page 52: Bloodborne Pathogens in the UNC-CH Laboratory Setting

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52

Personal Protective Equipment

• Specialized clothing or equipment worn by an employee for protection against a hazard

• General work clothes (including uniforms) are not PPE

• It is UNC’s responsibility as an employer to provide PPE in the appropriate sizes that is readily accessible to your worksite or issued to you as a properly trained employee.

Page 53: Bloodborne Pathogens in the UNC-CH Laboratory Setting

Whenever your duties create the potential for exposure to blood or OPIM, personal protective equipment such as gloves and eye protection for cleaning spills must be available and utilized.

If a garment(s) is penetrated by blood or OPIM, the garment(s) must be removed immediately or as soon as feasible.

Personal Protective Equipment

53Questions?? Call 962-5507 between 8 a.m.

& 5 p.m.

Page 54: Bloodborne Pathogens in the UNC-CH Laboratory Setting

LAUNDRY

What to do if clothing becomes soiled with blood or OPIM?

1. Handle uniforms soiled with blood as little as possible, using gloves and appropriate protective clothing.

2. Place garment soiled with blood in bags that prevent leakage.

3. Contaminated uniforms cannot be taken home for cleaning. Facilities Services employees should contact Mark Baker (2-1261) so that arrangements can be made for laundering.

Questions?? Call 962-5507 between 8 a.m. & 5 p.m.

54

Page 55: Bloodborne Pathogens in the UNC-CH Laboratory Setting

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55

RESPONDING TO AN INCIDENT IN YOUR LAB

Page 56: Bloodborne Pathogens in the UNC-CH Laboratory Setting

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56

Now that we’ve discussed what it means to be exposed, let’s look what to do in the event of a potential exposure.

An important part of this training program is to make sure all lab workers know the following:

(1) if they have had an exposure incident and

(2) what to do after they have an exposure incident.

RESPONDING TO AN INCIDENT IN YOUR LAB

Page 57: Bloodborne Pathogens in the UNC-CH Laboratory Setting

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57

What is a BBP Exposure?The OSHA BBP Standard Definition:

“A specific eye, mouth, other mucous membrane, non-intact skin or parenteral contact with blood or OPIM that results from the performance of an employee’s duties.”

Also, remember:

UNC requires you to report ANY incident resulting in injury from the performance of your duties.

BBP Exposure = Blood, OPIM contact with: Eye,

Nose,Mouth,

Shaving cut, rash, etc.

Page 58: Bloodborne Pathogens in the UNC-CH Laboratory Setting

Questions?? Call 962-5507 between 8 a.m. & 5 p.m. 58

What is a BBP Exposure?

• When blood or OPIM enter an unprotected break in your skin such as an open wound, scratch, acne, rash; or

• When blood or OPIM splashes or gets into your eyes, nose, or mouth; or

• If you are cut or stuck by an object (it must break the skin) that is contaminated with blood or OPIM.

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Immediate Precautions

What to do for needlesticks, cuts from contaminated objects, animal bites or scratches:

1. Remove contaminated gloves and if possible, allow the wound to bleed freely for a minute.

2. Wash the wound with soap and water for 5 minutes and apply sterile gauze or a bandage, if necessary.

3. Decontaminate and remove protective lab clothing and proceed immediately to UEOHC or call HealthLink. If the injury requires immediate medical attention, go to the Emergency Room.

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Immediate Precautions

1. Rinse tissue surface with copious amounts of water.

2. Eyes should be irrigated for at least 5 minutes using the emergency eyewash station.

3. Decontaminate and remove protective lab clothing and proceed immediately to UEOHC or call HealthLink.

What to do for mucous membrane exposure (eyes, nose, or mouth):

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Report Exposures Immediately

After any exposure event during work hours, employees must be seen by the University Employee Occupational Health Clinic (UEOHC) for treatment and documentation of exposure. After hours, all calls to the UEOHC are routed to HealthLink (966-7890) through UNC Hospitals. You should request that the MD on call for UEOHC after hours bloodborne pathogen exposures be called. The on-call MD will determine the need for immediate prophylaxis and if needed, direct the worker to meet him/her in the ER, or otherwise arrange for appropriate blood tests to be drawn and medications to be dispensed.

Immediately report the incident to your supervisor and/or call the University Employee Occupational Health (UEOHC) at 6-9119.

Emergency Contact Numbers

UEOHC Clinic: 966-9119

Emergency: 9-911

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Post-Exposure Evaluation

Following the incident, UNC will provide you with a post exposure medical evaluation.

This evaluation may include:

• Documenting routes of exposure

• Documenting circumstances of the incident

• Identifying sources of contamination

• Blood tests for HIV, HBV with consent from employee

• Post exposure prophylaxis and counseling

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Post Exposure Evaluation

1. The Principal Investigator and EHS must be notified of all exposures.

2. A NCIC Form 19 must be completed by the employee at UEOHC

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Safety Resources

Below is a list of Safety Resources that can provide you with more safety information.

How to Protect Yourself from Needlestick Injury (NIOSH)

http://www.cdc.gov/niosh/docs/2000-135/pdfs/2000-135.pdf

Selecting, Evaluating, and Using Sharps Disposal Containers http://www.cdc.gov/niosh/docs/97-111/

Bloodborne Infectious DiseasesHIV/AIDS, Hepatitis B Virus, and Hepatitis C Virus

http://www.cdc.gov/niosh/topics/bbp/

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CDC & OSHA Standards

OSHA on Bloodborne PathogensTopics Page

http://www.osha.gov/SLTC/bloodbornepathogens/index.html

OSHA's Bloodborne Pathogens Standardhttp://www.osha.gov/pls/oshaweb/owadisp.show_document?p_table=STANDARDS&p_id=10051

Exposure to Blood: What Health Care Workers Need to Know (CDC)

http://www.cdc.gov/ncidod/dhqp/pdf/bbp/exp_to_blood.pdf

Sharps Safety: Workbook for Designing, Implementing, and Evaluating a Sharps Injury Prevention Program (CDC

http://www.cdc.gov/sharpssafety/

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Questions??

• If you have any questions, please contact Environment, Health and Safety at 962-5507 between 8 am and 5 pm.

Don’t forget to take the exam to get credit for this course!

To begin the exam, go tohttps://itsapps.unc.edu/SelfStudyUnits/?testid=38

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