biovet catalog of products
DESCRIPTION
Veterinary High performance animal diagnostic kits for laboratory, clinical and on site use. Bovicheck®, Poultry Check®, Swinecheck®, Ovucheck®, Entericheck®, Pathasure Enteritis®, etc.TRANSCRIPT
4375, av. Beaudry, Saint-Hyacinthe QC J2S 8W2 CANADA Phone 450 771-7291 • Fax 450 771-4158
www.biovet.ca
1-888-8BIOVET (824-6838)
About Biovet
Biovet operates a USDA and CFIA certified laboratory offering a complete array of innovative diagnostic services for veterinarians. More than a reference in Veterinary diagnostic, our laboratory is accredited by SCC and MSDEFCC for microbiological analyzes on foods and drinking water and Non-drinking water.
Additionally, Biovet is the only Canadian company to develop, manufacture and market high performance animal diagnostic kits. The head office is located in Saint-Hyacinthe, within the city of biotechnology, with a subsidiary in the United States.
For further information, visit our web site at www.biovet.ca or contact us at:
USA1-877-8BIOVET
Canada & International 1-888-8BIOVET
AG
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DIAGNOSTIC KITS
AGRI-FOOD xMAP® Salmonella Serotyping Assay Kit by Luminex®
AVIAN Poultry Check MP® MS-MG
Poultry Check MP® MS-MG-AI
xMAP® Flock Monitor IBDV-NDV-IBV-REO by Luminex®
BOVINE Bovichek® BoHV-1 gB (IBR)
Bovichek® Lepto HP
Bovichek® Leukemia – 2 or 5 plates
Bovichek® M. bovis (Improved) – 1 or 2 plate
Bovichek® M. avium ssp paratuberculosis (Johne’s)
Bovichek® Neospora
Bovine ENTERICHEK®
Bovine ENTERICHEK® CRYPTO
BVDV Ag – 2 or 5 plates
FMDV NSP-3B cELISA (multi-species)
Pathasure Enteritis 3
Pathasure Enteritis 4
SWINE
Swinecheck MP® APP 1-9-11, 2, 3-6-8-15, 4-7, 5, 10 and 12
Swinecheck MP® APP 1-9-11, 2, 3-6-8-15, 4-7, and 5
Swinecheck MP® PRRS (NA + EU), SIV, PCV2
(other Elisa kits)
FMDV NSP-3B cELISA (multi-species)
Swinecheck® APP 1-9-11
Swinecheck® APP 2
Swinecheck® APP 3, 6, 8
Swinecheck® APP 4, 7
Swinecheck® APP 5a, 5b
Swinecheck® Mix-APP
All kits available internationally with the following permit requirements:
CFIA-licensed;
CFIA-licensed for export – requires a permit to release veterinary biologics from CFIA in Canada;
Requires a permit to release veterinary biologics from CFIA in Canada. This product is not licensed by CFIA and any claims made have not been substantiated by CFIA;
USDA approved;
Requires a Research and Evaluation import permit from the USDA in the US. This product is not licensed by the USDA and any claims made have not been substantiated by the USDA.
NEW SOON AVAILABLE
CFIA: Canadian Food Inspection Agency;
MSDEFCC: ministry of sustainable Development, Environment and the Fight against Climate Change;
SCC: Standards Council of Canada;
USDA: United States Department of Agriculture.
Rev. 2015-12-15
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SWINE (other Elisa kits) — [Continued]
Swinecheck® APP 10
Swinecheck® APP 12
Swinecheck® Mix-APP 10-12
Swinecheck® H1N1
Swinecheck® H3N2
Swinecheck® HPS
Swinecheck® PCV2 indirect – 2 or 5 plates
Swinecheck® PED indirect – 2 or 5 plates
Swinecheck® PRRSV type 1 and 2
Swinecheck® TGEV – 2 or 5 plates
Swinecheck® TGEV-PRCV – 2 or 5 plates
OVUCHECK® (Progesterone measurement) Ovucheck® Milk
Ovucheck® Plasma
Ovucheck® Premate 10-5
Ovucheck® Premate porcine
Ovucheck® Rapid Well
Ovucheck® Reader
OTHERS
FMDV NSP-3B cELISA (multi-species)
Dermatophytes
Bio-Tubes: Blood sample test kit
Sampling Kit for collection of oral fluids
Diagnostic kits
-
Diagnostic kits
2014-10-22
xMAP® Salmonella Serotyping Assay Kit by
PRODUCT CODE: TRD-502
DESCRIPTION
The Luminex® xMAP® Salmonella Serotyping Assay (SSA) is a multiplex, nucleic acid-based assay for research or epidemiological use in identifying the serotype of a Salmonella isolate. It is not for diagnosis of Salmonella spp. contamination or infection. This assay has been evaluated with Salmonella isolates only.
SUMMARY AND EXPLANATION
The xMAP Salmonella Serotyping Assay is a microsphere-based, molecular serotyping assay that detects genes involved in the expression of serotype-specific antigens. To determine a Salmonella serotype, the somatic O antigen and all phases of the motility H antigen must be identified. By detecting the genes involved in antigen expression rather than the antigens directly, as in traditional plate or tube agglutination, rough and more problematic isolates can be identified quickly and without the need for motility antigen-phase inversion. Molecular based serotyping removes the subjectivity of agglutination analysis and is not prone to common issues such as antisera lot-to-lot inconsistency. The assay serotypes approximately 85% of the top 100 serotypes most commonly encountered in testing laboratories, while providing partial serotype information for many other serotypes. These serotypes represent greater than 90% of the serotypes routinely encountered in public health laboratory settings.
CHARACTERISTICS
Molecular assay with results that are identical to traditional serotyping (Kauffman-White Scheme)
Ability to serotype rough and problematic isolates
No phase inversions required
Increased reliability and efficiency with decreased costs
Removes subjectivity
Eliminates antisera lot to lot variance
Time to result: ~ 4 hrs vs. days/weeks
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
ASSAY PROCEDURE TIME
1. DNA extraction 30 minutes
2. Amplification of O, H and AT genes 3 hours
3. Hybridization with Luminex beads 45 minutes
4. Add reporter and incubate 10 minutes
5. Plate reading
6. Data analysis
Diagnostic kits
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2014-12-23
Poultry Check MP® MS-MG
PRODUCT CODE: TRM-555
DESCRIPTION
Poultry Check MP® Ms-Mg Assay is a multiplexed fluorometric immunoassay (MFIA) intended for detecting antibodies to Mycoplasma synoviae (MS) and Mycoplasma gallisepticum (MG) in chicken serum.
SUMMARY AND EXPLANATION
Mycoplasma synoviae (MS) and Mycoplasma gallisepticum (MG) are two major bacterial pathogens for poultry flocks. The surveillance of poultry flocks for MS and MG relies on regular testing of serum samples for the presence of specific antibodies.
CHARACTERISTICS
Multiplexed fluorometric immunoassay (MFIA)
Results in less than 2 hours
460 tests per kit ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Preparation of samples and reagents
2. Incubation of samples and controls 60 minutes / 19°C to 26°C
3. Serial washings
4. Incubation of conjugate 15 minutes / 19°C to 26°C
5. Serial washings
6. Revelation with substrate 15 minutes / 19°C to 26°C
7. Serial washings
8. Plate reading with a special reader
IMPORTANT :
Requires a permit to release veterinary biologics from CFIA in Canada. This product is not licensed by CFIA and any claims made have not been substantiated by CFIA.
Requires a Research and Evaluation import permit from the USDA in the US. This product is not licensed by the USDA and any claims made have not been substantiated by the USDA.
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2014-12-23
xMAP® Flock Monitor IBDV-NDV-IBV-REO by
PRODUCT CODE: TRD-500
DESCRIPTION
The Luminex® xMAP® Flock Monitor Assay is a mutiplex immunoassay intended for determining the presence of antibodies for Infectious Bursal Disease Virus (IBDV), Newcastle Disease Virus (NDV), Infectious Bronchitis Virus (IBV), and Avian Reovirus (REO) in chicken serum. This test kit is not indicated for diagnosis of disease in individidual birds.
SUMMARY AND EXPLANATION
Serological testing is routinely used to monitor antibody response to vaccination against Infectious Bursal Disease Virus (IBDV), Newcastle Disease Virus (NDV), Infectious Bronchitis Virus (IBV), and Avian Reovirus (REO). Luminex® xMAP® Flock Monitor allows determining antibody titers against these four viruses in a single assay.
CHARACTERISTICS
Multiplexed fluorometric immunoassay (MFIA)
Results in less than 2 hours
460 tests per kit
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Preparation of samples and reagents
2. Incubation of samples and controls with the bead mix
60 minutes / 19°C to 26°C
3. Washings
4. Incubation of the detection antibody 30 minutes / 19°C to 26°C
5. Washings
6. Incubation of the reporter 30 minutes / 19°C to 26°C
7. Washings
8. Resuspending beads
9. Plate reading
10. Data analysis
IMPORTANT :
Approved by USDA.
Requires a permit to release veterinary biologics from CFIA in Canada. This product is not licensed by CFIA and any claims made have not been substantiated by CFIA.
Diagnostic kits
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2014-12-23
BOVICHEK® BoHV-1 gB
PRODUCT CODE: TRM-550
DESCRIPTION
This kit is based on an immunoenzymatic assay for the detection of antibodies against bovine herpes virus 1 (BoHV-1) in bovine serum using BoHV-1-gB specific monoclonal antibodies.
SUMMARY AND EXPLANATION
Infectious bovine rhinotracheitis (IBR) is a highly contagious disease caused by BoHV-1. The virus which affects domestic and wild cattle is present worldwide but has been eradicated from several countries. The disease is characterized by clinical signs of the upper respiratory tract (rhinitis, conjunctivitis, tracheitis) accompanied by signs of general illness (fever, depression, inappetence, abortion). Moreover the virus can also infect the genital tract and cause pustular vulvovaginitis and balanoposthitis. Mortality is usually low and numerous infections are subclinical. Following infection the virus persists in a latent state for the entire life of the animals. It can be occasionally reactivated eventually resulting in virus shedding without clinical signs.
CHARACTERISTICS
2 x 96 tests
Sensitive, specific, and reproducible
Rapid (about 2 hrs 30)
Easy to perform
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Dispense samples and controls and incubate 1 hour at 37°C
2. Wash
3. Dispense conjugate and incubate 1 hour at 37°C
4. Wash
5. Dispense substrate and incubate 15 minutes at room temperature
6. Dispense stop solution and read at 450 nm
IMPORTANT :
Requires a permit to release veterinary biologics from CFIA in Canada. This product is not licensed by CFIA and any claims made have not been substantiated by CFIA.
Requires a Research and Evaluation import permit from the USDA in the US. This product is not licensed by the USDA and any claims made have not been substantiated by the USDA.
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2014-12-23
BOVICHEK® Lepto HP
PRODUCT CODE: TRM-533
DESCRIPTION
This is an immunoenzymatic assay for the detection of antibodies against Leptospira pomona and hardjo in bovine serum.
SUMMARY AND EXPLANATION
Controls and bovine serum samples are incubated in wells coated with either L. pomona or L. hardjo antigens (Ag). The antibodies (Ab) specific to L. pomona or L. hardjo present in positive serum samples will bind the Ag in the wells. After several washes to eliminate unbound substances, a conjugate (protein G coupled to peroxydase) is added. After incubation the excess of this conjugate is eliminated by a second series of washes and its attachment to bovine antibodies is revealed with a chromogenous substrate. The conjugate, if present, reacts with the substrate and a blue color develops. The reaction is then stopped and the optical densities are read. The intensity of the color is proportional to the quantity of Ab in the serum samples.
CHARACTERISTICS
Indirect ELISA
Specific antigens of Leptospira Hardjo and Pomona
Fast execution: less than 2 hours
96 tests for Leptospira Hardjo et Pomona kit
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Serum dilution
2. Samples incubation and control 30 minutes at 23 + 2°C
3. Successive washes
4. Conjugate incubation 30 minutes at 23 + 2°C
5. Successive washes
6. Reading with substrate 15 minutes at 23 + 2°C
7. Stopping enzymatic reaction
8. Measuring optical densities
9. Calculation and interpretation of results
IMPORTANT :
Requires a permit to release veterinary biologics from CFIA in Canada. This product is not licensed by CFIA and any claims made have not been substantiated by CFIA.
Requires a Research and Evaluation import permit from the USDA in the US. This product is not licensed by the USDA and any claims made have not been substantiated by the USDA.
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2015-04-30
BOVICHEK® Leukemia – 2 or 5 plates
PRODUCT CODE: TRM-506/509
DESCRIPTION
This kit is an immunoenzymatic assay intended for the detection of Bovine Leukemia Virus (BLV) antibodies in cattle serum and milk.
SUMMARY AND EXPLANATION
BLV is a retrovirus which causes enzootic bovine leukosis (EBL). Most infections with BLV are subclinical, but a proportion of adult cattle develop persistent lymphocytosis, and eventually lymphosarcomas (tumours) in various organs. Clinical signs, if present, depend on the organs affected.
BLV is present in blood lymphocytes and in tumour cells as provirus integrated into the DNA of the cell. It is also found in the cellular fraction of various body fluids (e.g. saliva, milk). Natural transmission depends on the transfer of infected cells (e.g. during parturition). Some blood-sucking insects may also transmit the virus mechanically. Artificial transmission occurs, especially by blood-contaminated needles, surgical equipment, gloves used for rectal examinations, etc.
Infection with BLV in cattle gives rise to a persistent antibody response. Antibodies can first be detected 3-16 weeks after infection. They are present in both serum and milk. Maternally derived antibodies may take up to 6 or 7 months to disappear. The antibodies most readily detected are those directed towards the envelope glycoprotein gp51 of the virus.
Routine diagnosis of BLV infection is based on the detection of specific antibodies. Serological testing is used in certification programs, eradication programs and for commercial exchanges.
CHARACTERISTICS
Blocking ELISA: antigens specific to BLV (gp 51 protein)
Rapid (less than 2 hours)
Tests per kit: o 2 plates: up to 184 samples tested individually or pooled o 5 plates: up to 460 samples tested individually or pooled
(up to 10 serum samples or 2 milk samples per pool)
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Preparation of samples
2. Incubation of samples and controls 1h00 / 37ºC
3. Successive washes
4. Incubation with conjugate 30 min / 37ºC
5. Successive washes
6. Revelation with substrate 10 min / room temperature
7. Stop the reaction
8. Reading optical densities 450 nm
IMPORTANT :
Requires a permit to release veterinary biologics from CFIA in Canada. This product is not licensed by CFIA and any claims made have not been substantiated by CFIA.
Requires a Research and Evaluation import permit from the USDA in the US. This product is not licensed by the USDA and any claims made have not been substantiated by the USDA.
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2014-12-23
BOVICHEK® M. avium ssp paratuberculosis (Johne’s)
PRODUCT CODE: TRM-524
DESCRIPTION
This is an immunoenzymatic assay for the detection of antibodies against Mycobacterium avium ssp paratuberculosis (Map) in bovine serum.
SUMMARY AND EXPLANATION
Map is the etiological agent of Johne’s disease (synonym: paratuberculosis). This disease affects several ruminant species. Map multiplies in the intestinal mucosa and induces a progressive enteritis resulting in chronic diarrhea, wasting, drop in milk production and eventually death. The diagnosis of Johne’s is based on the demonstration of Map in the feces or tissues or of Map specific antibodies in the serum or milk. The most sensitive serological assays to detect Map specific antibodies are enzyme linked immune enzymatic assays (ELISA). The sensitivity of these tests increases with the progression of the infection. Some animals free from Map have antibodies directed against other environmental mycobacteria that may cause non-specific reactions. Pre-treatment of their sera with selected mycobacteria allows removing these non-specific antibodies.
CHARACTERISTICS
Test per kit: up to 92 samples
Indirect ELISA
Absorption of the samples with selected mycobacteria
Results within two hours
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Incubation of the samples with the absorbent
30 minutes at 23°C ± 2°C
2. Incubation of the absorbed samples 30 minutes at 23°C ± 2°C
3. Successive washings
4. Incubation with the conjugate 30 minutes at 23°C ± 2°C
5. Successive washings
6. Stop the reaction
7. Incubation with the substrate 30 minutes at 23°C ± 2°C
8. Plate reading (450 nm)
IMPORTANT :
Requires a permit to release veterinary biologics from CFIA in Canada. This product is not licensed by CFIA and any claims made have not been substantiated by CFIA.
Requires a Research and Evaluation import permit from the USDA in the US. This product is not licensed by the USDA and any claims made have not been substantiated by the USDA.
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2015-02-26
BOVICHEK® M. bovis – 1 or 2 plates
PRODUCT CODE: TRM-501/502
DESCRIPTION
This is an immunoenzymatic assay for the detection of antibodies against Mycoplasma bovis (M.bovis) in bovine serum and milk.
SUMMARY AND EXPLANATION
Epidemiologic studies have revealed the pathogenic properties of M. bovis in cattle. M. bovis is one of the pathogens responsible for mastitis in the cow and pneumopathy in the calf. M. bovis is also involved in abortion. Serodiagnosis is a valuable tool to diagnose M. bovis infections since immune response is early, important, stable, and persistent in time.
CHARACTERISTICS
Indirect ELISA: Specific antigens to M. Bovis
Interpretation based on positivity level
Rapid (less than 2 hours)
Tests per kit: o 1 plate: 48 samples tested as duplicate o 2 plates: 96 samples tested as duplicate
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Preparation of samples
2. Incubation of samples and controls 30 min / 23°C
3. Successive washes
4. Incubation with conjugate 30 min / 23°C
5. Successive washes
6. Revelation with substrate 10 min / 23°C
7. Stop the reaction
8. Reading optical densities 450 nm
IMPORTANT :
Requires a permit to release veterinary biologics from CFIA in Canada. This product is not licensed by CFIA and any claims made have not been substantiated by CFIA.
Requires a Research and Evaluation import permit from the USDA in the US. This product is not licensed by the USDA and any claims made have not been substantiated by the USDA.
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2015-12-15
BOVICHEK® Neospora
PRODUCT CODE: TRM-500
DESCRIPTION
This is an immunoenzymatic assay for the detection of antibodies against Neospora caninum in bovine serum and milk.
SUMMARY AND EXPLANATION
Neospora caninum is a protozoa identified by Dubey et al. in 1988. In cattle, this protozoa is responsible for an important proportion of spontaneous abortions. Once the animal is infected, the chance of it undergoing abortion is 2- to 3-fold greater than in noninfected animals. Furthermore, if abortion does not occur, the animal will likely, 8 times out of 10, give birth to infected cattle.
CHARACTERISTICS
Indirect ELISA: Specific antigens to N. caninum (Strain NC-1)
Rapid (less than 2 hours)
Maximum number of samples tested per kit : 2 x 90
When considering suspicious results as positive: - the relative sensitivity of this test is 99.4% (95% CI = 96.1 – 100%) - the relative specificity is 99.1% (95% CI = 98.1 – 99.6%).
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Preparation of samples
2. Incubation of samples and controls 45 min / 37°C
3. Successive washes
4. Incubation with conjugate 30 min / 37°C
5. Successive washes
6. Revelation with substrate 15 min / 23°C
7. Stop the reaction
8. Reading optical densities 405 nm and reference filter at 490 nm
IMPORTANT :
Requires a permit to release veterinary biologics from CFIA in Canada. This product is not licensed by CFIA and any claims made have not been substantiated by CFIA.
Requires a Research and Evaluation import permit from the USDA in the US. This product is not licensed by the USDA and any claims made have not been substantiated by the USDA.
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2014-10-20
PRODUCT CODE: TRM-504
APPLICATION
BOVINE ENTERICHEK is a lateral flow immunochromatography assay allowing to detect Escherichia coli K99, Rotavirus, Coronavirus or Cryptosporidium in bovine feces present in their environment. Each kit contains enough reagents to examine 5 fecal samples for each of the 4 above mentioned agents. The test is particularly user-friendly and can be performed on the farm itself.
DISEASE DESCRIPTION
Neonatal diarrhea is a major disease of calves. The most important infectious agents causing the disease are Rotavirus, Coronavirus, Escherichia coli K99 and Cryptosporidium. Identifying these agents in fecal samples allows to determine to which agents calves are exposed and to take appropriate control measures.
CHARACTERISTICS
Lateral flow immunochromatography assay
Qualitative assay
Reagents ready to use
Rapid (15 minutes)
Tests per kit : 5 tests for 4 agents
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Dilute fecal samples
2. Immerse strips into diluted fecal samples 10 min, room temperature
3. Visual reading
Trousses diagnostiques
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2014-11-21
PRODUCT CODE: TRM-511
APPLICATION
La trousse ENTERICHECK CRYPTO est un test d’immunochromatographie latérale qui permet de détecter la présence de Cryptosporidium dans les matières fécales qui sont présentes dans leur environnement. Chaque trousse contient les réactifs suffisants pour examiner 10 échantillons de matières fécales. Grâce à la facilité d’emploi de la trousse, les tests peuvent être réalisés sur la ferme même.
DESCRIPTION DE LA MALADIE
Cryptosporidium est un des principaux agents de diarrhée néonatale chez le veau. L’identification de cet agent dans les matières fécales permet de déterminer ceux auxquels les veaux sont exposés afin de prendre les mesures de contrôle appropriées.
CARACTÉRISTIQUES
Test d’immunochromatographie latérale
Test qualitatif
Composantes prêtes à l’emploi
Exécution rapide : 15 minutes
Nombre de tests par trousse : 10
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Diluer les matières fécales
2. Immerger les bandelettes dans les matières fécales diluées.
10 min, température de la pièce
3. Lecture visuelle
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2015-04-30
BVDV Ag – 2 or 5 plates
PRODUCT CODE: TRM-508/510
DESCRIPTION
This kit is an immunoenzymatic assay intended for the detection of Bovine Viral Diarrhea virus (BVDV) in tissues and blood of persistently infected cattle.
SUMMARY AND EXPLANATION
Bovine Viral Diarrhea virus (BVDV) is responsible for a variety of economically important disorders in cattle (diarrhea, abortion, pneumonia, etc.). Foetal infections with BVDV during the first trimester of pregnancy can result in the birth of persistently infected (PI) calves. Such animals excrete large quantities of the virus throughout their entire life. Removal of PI animals is the corner stone of efficient BVDV control programs.
CHARACTERISTICS
Capture ELISA: specific detection of BVDV antigens
Rapid: less than 3.5 hours
Tests per kit:
o 2 plates: 192 tests
o 5 plates: 460 tests
PROCÉDURE GÉNÉRALE TEMPS D’INCUBATION/ TEMP.
1. Preparation of samples 15-30 minutes
2. Incubation of samples and controls 1 hour/room temperature
3. Successive washes
4. Incubation with first conjugate 1 hour/room temperature
5. Successive washes
6. Incubation with second conjugate 1 hour/room temperature
7. Successive washes
8. Revelation with substrate 5 minutes/room temperature
9. Reading the results
IMPORTANT :
CFIA-licensed.
Requires a Research and Evaluation import permit from the USDA in the US. This product is not licensed by the USDA and any claims made have not been substantiated by the USDA.
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2014-12-23
Pathasure Enteritis 3
PRODUCT CODE: TRM-503
DESCRIPTION
This kit is an immunoenzymatic assay intended for the detection of Rotavirus, Coronavirus and Escherichia coli (E. coli) K99 antigens in bovine feces.
SUMMARY AND EXPLANATION
Diarrhea problems in the calf are often caused by Rotavirus, Coronavirus, or E. coli K99. These problems can lead to important economical losses since affected animals generally show reduced weight gain, sometimes resulting in death. Quick identification of the pathogen involved helps in disease control. It is recommended to test the feces of at least 3 calves per infected herd throughout symptom evolution.
CHARACTERISTICS
Indirect ELISA: Specific Antibodies to Rotavirus, Coronavirus and E. coli K99
Rapid (Less than 2 hours)
Qualitative test
Test per kit : 1 well is needed to test each agent Example: 30 fecal samples can be tested for the 3 agents 94 fecal samples can be tested for 1 agent only
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Preparation of samples
2. Incubation of samples and controls 30 min / 23°C
3. Successive washes
4. Incubation with conjugates 30 min / 23°C
5. Successive washes
6. Revelation with substrates 15 min / 23°C
7. Reading the results Visual
IMPORTANT :
CFIA-licensed for export – requires a permit to release veterinary biologics from CFIA in Canada.
Requires a Research and Evaluation import permit from the USDA in the US. This product is not licensed by the USDA and any claims made have not been substantiated by the USDA.
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2014-12-23
Pathasure Enteritis 4
PRODUCT CODE: TRM-507
DESCRIPTION
This kit is an immunoenzymatic assay intended for the detection of Rotavirus, Coronavirus, Escherichia coli (E.coli) K99 and Crytosporidium parvum antigens in bovine feces.
SUMMARY AND EXPLANATION
Diarrhea problems in the calf are often caused by Rotavirus, Coronavirus, E. coli K99 or Cryptosporidium parvum. These problems can lead to important economical losses since affected animals generally show reduced weight gain, sometimes resulting in death. Quick identification of the pathogen involved helps in disease control. It is recommended to test the feces of at least 3 calves per infected herd throughout symptom evolution.
CHARACTERISTICS
Indirect ELISA: Specific Antibodies to Rotavirus, Coronavirus, E. coli K99, Cryptosporidium parvum
Rapid (Less than 2 hours)
Qualitative test
Test per kit : 1 well is needed to test each agent Example: 22 fecal samples can be tested for the 4 agents 94 fecal samples can be tested for 1 agent only
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Preparation of samples
2. Incubation of samples and controls 30 min / 23°C
3. Successive washes
4. Incubation with conjugates 30 min / 23°C
5. Successive washes
6. Revelation with substrates 15 min / 23°C
7. Reading the results Visual
IMPORTANT :
CFIA-licensed for export – requires a permit to release veterinary biologics from CFIA in Canada.
Requires a Research and Evaluation import permit from the USDA in the US. This product is not licensed by the USDA and any claims made have not been substantiated by the USDA.
Diagnostic kits
SW IN E
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2015-04-30
SWINECHECK MP® APP 1-9-11, 2, 3-6-8-15, 4-7, and 5
PRODUCT CODE: TRM-557
DESCRIPTION
Swinecheck MP® APP assay is a mutiplex immunoassay intended for determining the presence of antibodies to Actinobacillus pleuropneumoniae (APP) serogroups 1-9-11, 2, 3-6-8-15, 4-7, and 5 in swine serum.
SUMMARY AND EXPLANATION
Swinecheck MP® APP assay is a microsphere-based multiplexed fluorometric immunoassay (MFIA)-type antibody detection test. A total of 5 distinct microsphere sets (coated with either APP 1-9-11, APP 2, APP 3-6-8-15, APP 4-7, or APP 5 antigens) are incubated in diluted swine serum and then washed. Successive incubations with biotinylated anti-swine IgG (Detection Antibody) and streptavidin R-phycoerythrin (SA-PE) reporter follow, each succeeded by a wash step to remove unbound materials. Users place plates in the plate analyzer, which captures the fluorescence intensity of the microspheres and SA-PE reporter. An S/P ratio of the SA-PE on the antigen-coated microspheres above the provided cut-offs indicates antibody is present in the sample.
CHARACTERISTICS
Multiplexed fluorometric immunoassay (MFIA)
5 plates
Results available within 2.5 hours
Tests per kit: up to 445 samples
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Preparation of samples
2. Incubation of samples and controls 1hr / 19-26°C
3. Serial washings
4. Incubation with conjugate A 30 min / 19-26°C
5. Serial washings
6. Incubation with conjugate B 30 min / 19-26°C
7. Serial washings
8. Plate reading
IMPORTANT :
Requires a permit to release veterinary biologics from CFIA in Canada. This product is not licensed by CFIA and any claims made have not been substantiated by CFIA.
Requires a Research and Evaluation import permit from the USDA in the US. This product is not licensed by the USDA and any claims made have not been substantiated by the USDA.
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2015-12-17
SWINECHECK MP® PRRSV SIV and PCV2
PRODUCT CODE: TRM-554
DESCRIPTION
Swinecheck MP® PRRSV, SIV, PCV2 assay is a mutiplex immunoassay intended for determining the presence of antibodies to Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) type 1 and 2, Swine Influenza Virus (SIV) type A, and Porcine Circovirus type 2 (PCV2) in swine serum.
SUMMARY AND EXPLANATION
Swinecheck MP® PRRSV, SIV, PCV2 assay is a microsphere-based multiplex fluorescent immunoassay (MFIA)-type antibody detection test. A total of 4 distinct microsphere sets [coated with either PRRSV NA (type 2), PRRSV EU (type 1), SIV, and PCV2 antigens] are incubated in diluted swine serum and then washed. Successive incubations with biotinylated anti-swine IgG (Detection Antibody) and streptavidin R-phycoerythrin (SA-PE) reporter follow, each succeeded by a wash step to remove unbound reagent. Users place plates in the plate analyzer, which captures the fluorescence intensity of the microspheres and SA-PE reporter. An S/P ratio of the SA-PE on the antigen-coated microspheres above the provided cut-offs indicates antibody is present in the sample.
CHARACTERISTICS
Multiplexed fluorometric immunoassay (MFIA)
5 plates
Results available within 2.5 hours
Tests per kit: up to 445 samples
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Preparation of samples
2. Incubation of samples and controls 1hr / 19-26°C
3. Serial washings
4. Incubation with secundary antibodies 30 min / 19-26°C
5. Serial washings
6. Incubation with fluorescing reporter 30 min / 19-26°C
7. Serial washings
8. Plate reading
IMPORTANT :
Requires a permit to release veterinary biologics from CFIA in Canada. This product is not licensed by CFIA and any claims made have not been substantiated by CFIA.
Requires a Research and Evaluation import permit from the USDA in the US. This product is not licensed by the USDA and any claims made have not been substantiated by the USDA.
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2015-04-30
SWINECHECK® APP 1-9-11
PRODUCT CODE: TRM-514
DESCRIPTION
This is an immunoenzymatic assay for the detection of antibodies against Actinobacillus pleuropneumoniae serotypes 1, 9, 11 in porcine serum.
SUMMARY AND EXPLANATION
Pleuropneumonia caused by Actinobacillus pleuropneumoniae serotypes 1, 9, 11 is an important porcine disease that can result in considerable economic losses. Herds are often chronically infected without clinical symptoms. However, following a stressful situation, several clinical cases with mortality can occur. Since horizontal transmission through airways is quite important and infection is usually inapparent, serology becomes an indispensable tool for its detection.
CHARACTERISTICS
Indirect ELISA: Specific antigens to serotypes 1, 9, 11
Rapid (Less than 2 hours)
Tests per kit: 184 samples
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Preparation of samples
2. Incubation of samples and controls 30 min / 23°C
3. Successive washes
4. Incubation of conjugate 30 min / 23°C
5. Successive washes
6. Revelation with substrate 23 min / 23°C
7. Stop the reaction
8. Reading the optical densities 405 nm (reference filter at 490 nm)
IMPORTANT :
CFIA-licensed for export – requires a permit to release veterinary biologics from CFIA in Canada.
Requires a Research and Evaluation import permit from the USDA in the US. This product is not licensed by the USDA and any claims made have not been substantiated by the USDA.
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2015-04-30
SWINECHECK® APP 2
PRODUCT CODE: TRM-516
DESCRIPTION
This is an immunoenzymatic assay for the detection of antibodies against Actinobacillus pleuropneumoniae serotype 2 in porcine serum.
SUMMARY AND EXPLANATION
Pleuropneumonia caused by Actinobacillus pleuropneumoniae serotype 2 is an important porcine disease that can result in considerable economic losses. Herds are often chronically infected without clinical symptoms. However, following a stressful situation, several clinical cases with mortality can occur. Since horizontal transmission through airways is quite important and infection is usually inapparent, serology becomes an indispensable tool for its detection.
CHARACTERISTICS
Indirect ELISA: Specific antigens to serotype 2
Rapid (Less than 2 hours)
Tests per kit: 184 samples
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Preparation of samples
2. Incubation of samples and controls 30 min / 23°C
3. Successive washes
4. Incubation of conjugate 30 min / 23°C
5. Successive washes
6. Revelation with substrate 23 min / 23°C
7. Stop the reaction
8. Reading the optical densities 405 nm (reference filter at 490 nm)
IMPORTANT :
CFIA-licensed for export – requires a permit to release veterinary biologics from CFIA in Canada.
Requires a Research and Evaluation import permit from the USDA in the US. This product is not licensed by the USDA and any claims made have not been substantiated by the USDA.
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2015-04-30
SWINECHECK® APP 3, 6, 8
PRODUCT CODE: TRM-517
DESCRIPTION
This is an immunoenzymatic assay for the detection of antibodies against Actinobacillus pleuropneumoniae serotypes 3, 6, 8 in porcine serum.
SUMMARY AND EXPLANATION
Pleuropneumonia caused by Actinobacillus pleuropneumoniae serotypes 3, 6, 8 is an important porcine disease that can result in considerable economic losses. Herds are often chronically infected without clinical symptoms. However, following a stressful situation, several clinical cases with mortality can occur. Since horizontal transmission through airways is quite important and infection is usually inapparent, serology becomes an indispensable tool for its detection.
CHARACTERISTICS
Indirect ELISA: Specific antigens to serotypes 3, 6, 8
Rapid (Less than 2 hours)
Tests per kit: 184 samples
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Preparation of samples
2. Incubation of samples and controls 30 min / 23°C
3. Successive washes
4. Incubation of conjugate 30 min / 23°C
5. Successive washes
6. Revelation with substrate 23 min / 23°C
7. Stop the reaction
8. Reading the optical densities 405 nm (reference filter at 490 nm)
IMPORTANT :
CFIA-licensed for export – requires a permit to release veterinary biologics from CFIA in Canada.
Requires a Research and Evaluation import permit from the USDA in the US. This product is not licensed by the USDA and any claims made have not been substantiated by the USDA.
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2015-04-30
SWINECHECK® APP 4, 7
PRODUCT CODE: TRM-518
DESCRIPTION
This is an immunoenzymatic assay for the detection of antibodies against Actinobacillus pleuropneumoniae serotypes 4, 7 in porcine serum.
SUMMARY AND EXPLANATION
Pleuropneumonia caused by Actinobacillus pleuropneumoniae serotypes 4, 7 is an important porcine disease that can result in considerable economic losses. Herds are often chronically infected without clinical symptoms. However, following a stressful situation, several clinical cases with mortality can occur. Since horizontal transmission through airways is quite important and infection is usually inapparent, serology becomes an indispensable tool for its detection.
CHARACTERISTICS
Indirect ELISA: Specific antigens to serotypes 4, 7
Rapid (Less than 2 hours)
Tests per kit: 184 samples
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Preparation of samples
2. Incubation of samples and controls 30 min / 23°C
3. Successive washes
4. Incubation of conjugate 30 min / 23°C
5. Successive washes
6. Revelation with substrate 23 min / 23°C
7. Stop the reaction
8. Reading the optical densities 405 nm (reference filter at 490 nm)
IMPORTANT :
CFIA-licensed for export – requires a permit to release veterinary biologics from CFIA in Canada.
Requires a Research and Evaluation import permit from the USDA in the US. This product is not licensed by the USDA and any claims made have not been substantiated by the USDA.
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2015-04-30
Swinecheck® APP 5a, 5b
PRODUCT CODE: TRM-515
DESCRIPTION
This is an immunoenzymatic assay for the detection of antibodies against Actinobacillus pleuropneumoniae serotypes 5a, 5b in porcine serum.
SUMMARY AND EXPLANATION
Pleuropneumonia caused by Actinobacillus pleuropneumoniae serotypes 5a, 5b is an important porcine disease that can result in considerable economic losses. Herds are often chronically infected without clinical symptoms. However, following a stressful situation, several clinical cases with mortality can occur. Since horizontal transmission through airways is quite important and infection is usually inapparent, serology becomes an indispensable tool for its detection.
CHARACTERISTICS
Indirect ELISA: Specific antigens to serotypes 5a, 5b
Rapid (Less than 2 hours)
Tests per kit: 184 samples
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Preparation of samples
2. Incubation of samples and controls 30 min / 23°C
3. Successive washes
4. Incubation of conjugate 30 min / 23°C
5. Successive washes
6. Revelation with substrate 23 min / 23°C
7. Stop the reaction
8. Reading optical densities 405 nm (reference filter at 490 nm)
IMPORTANT :
CFIA-licensed for export – requires a permit to release veterinary biologics from CFIA in Canada.
Requires a Research and Evaluation import permit from the USDA in the US. This product is not licensed by the USDA and any claims made have not been substantiated by the USDA.
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2015-04-30
SWINECHECK® Mix-APP
PRODUCT CODE: TRM-537
DESCRIPTION
This is an immunoenzymatic assay for the detection of antibodies against Actinobacillus pleuropneumoniae serotypes 1-2-9-11, 3-6-8-15 and 4-5-7 in porcine serum.
SUMMARY AND EXPLANATION
Pleuropneumonia caused by Actinobacillus pleuropneumoniae (APP) is an important swine disease that can result in considerable economic losses. Serological testing is a major diagnostic tool to detect herds infected with APP or to certify the absence of infection. There are presently 15 recognized serotypes of APP. As the economical importance of these serotypes varies significantly depending on the country, serological testing is usually limited to the most relevant serotypes and is based on serotype specific tests.
In herds considered to be free of several serotypes, a screening test able to simultaneously detect antibodies against the most relevant serotypes would simplify testing and reduce testing costs. In case of positive or suspect reactions, samples can be examined using relevant serotype specific tests.
CHARACTERISTICS
Indirect ELISA: Specific antigens to Actinobacillus pleuropneumoniae
Rapid (Less than 2 hours)
Test per kit: 180 samples tested for serotypes 1-2-9-11 and 4-5-7, 184 samples tested for serotypes 3-6-8-15
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Preparation of samples
2. Incubation of samples and controls 30 min / 23°C
3. Successive washes
4. Incubation of conjugate 30 min / 23°C
5. Successive washes
6. Revelation with substrate 23 min / 23°C
7. Stop the reaction
8. Reading the optical densities 405 nm (reference filter at 490 nm)
IMPORTANT :
Requires a permit to release veterinary biologics from CFIA in Canada. This product is not licensed by CFIA and any claims made have not been substantiated by CFIA.
Requires a Research and Evaluation import permit from the USDA in the US. This product is not licensed by the USDA and any claims made have not been substantiated by the USDA.
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2015-04-30
SWINECHECK® APP 10
PRODUCT CODE: TRM-519
DESCRIPTION
This is an immunoenzymatic assay for the detection of antibodies against Actinobacillus pleuropneumoniae serotype 10 in porcine serum.
SUMMARY AND EXPLANATION
Pleuropneumonia caused by Actinobacillus pleuropneumoniae serotype 10 is an important porcine disease that can result in considerable economic losses. Herds are often chronically infected without clinical symptoms. However, following a stressful situation, several clinical cases with mortality can occur. Since horizontal transmission through airways is quite important and infection is usually inapparent, serology becomes an indispensable tool for its detection.
CHARACTERISTICS
Indirect ELISA: Specific antigens to serotype 10
Rapid (Less than 2 hours)
Tests per kit: 184 samples
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Preparation of samples
2. Incubation of samples and controls 30 min / 23°C
3. Successive washes
4. Incubation of conjugate 30 min / 23°C
5. Successive washes
6. Revelation with substrate 23 min / 23°C
7. Stop the reaction
8. Reading the optical densities 405 nm (reference filter at 490 nm)
IMPORTANT :
Requires a permit to release veterinary biologics from CFIA in Canada. This product is not licensed by CFIA and any claims made have not been substantiated by CFIA.
Requires a Research and Evaluation import permit from the USDA in the US. This product is not licensed by the USDA and any claims made have not been substantiated by the USDA.
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2015-04-30
SWINECHECK® APP 12
PRODUCT CODE: TRM-520
DESCRIPTION
This is an immunoenzymatic assay for the detection of antibodies against Actinobacillus pleuropneumoniae serotype 12 in porcine serum.
SUMMARY AND EXPLANATION
Pleuropneumonia caused by Actinobacillus pleuropneumoniae serotype 12 is an important porcine disease that can result in considerable economic losses. Herds are often chronically infected without clinical symptoms. However, following a stressful situation, several clinical cases with mortality can occur. Since horizontal transmission through airways is quite important and infection is usually inapparent, serology becomes an indispensable tool for its detection.
CHARACTERISTICS
Indirect ELISA: Specific antigens to serotype 12
Rapid (Less than 2 hours)
Tests per kit: 184 samples
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Preparation of samples
2. Incubation of samples and controls 30 min / 23°C
3. Successive washes
4. Incubation of conjugate 30 min / 23°C
5. Successive washes
6. Revelation with substrate 23 min / 23°C
7. Stop the reaction
8. Reading the optical densities 405 nm (reference filter at 490 nm)
IMPORTANT :
Requires a permit to release veterinary biologics from CFIA in Canada. This product is not licensed by CFIA and any claims made have not been substantiated by CFIA.
Requires a Research and Evaluation import permit from the USDA in the US. This product is not licensed by the USDA and any claims made have not been substantiated by the USDA.
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2015-04-30
SWINECHECK® Mix-APP 10-12
PRODUCT CODE: TRM-538
DESCRIPTION
This is an immunoenzymatic assay for the detection of antibodies against Actinobacillus pleuropneumoniae serotypes 10-12 in porcine serum. It can be used in complement to kit Swinecheck® mix-APP 1-2-9-11, 3-6-8-15, and 4-5-7.
SUMMARY AND EXPLANATION
Pleuropneumonia caused by Actinobacillus pleuropneumoniae serotypes 10 and 12 is an important porcine disease that can result in considerable economic losses. Herds are often chronically infected without clinical symptoms. However, following a stressful situation, several clinical cases with mortality can occur. Since horizontal transmission through airways is quite important and infection is usually inapparent, serology becomes an indispensable tool for its detection.
CHARACTERISTICS
Indirect ELISA: Specific antigens against APP serotypes 10 and 12
Rapid (Less than 2 hours)
Test per kit: 184 samples
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Preparation of samples
2. Incubation of samples and controls 30 min / 23°C
3. Successive washes
4. Incubation of conjugate 30 min / 23°C
5. Successive washes
6. Revelation with substrate 23 min / 23°C
7. Stop the reaction
8. Reading the optical densities 405 nm (reference filter at 490 nm)
IMPORTANT :
Requires a permit to release veterinary biologics from CFIA in Canada. This product is not licensed by CFIA and any claims made have not been substantiated by CFIA.
Requires a Research and Evaluation import permit from the USDA in the US. This product is not licensed by the USDA and any claims made have not been substantiated by the USDA.
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2015-06-15
SWINECHECK® H1N1 – 2 or 5 plates
PRODUCT CODE: TRM-512
DESCRIPTION
This is an immunoenzymatic assay for the detection of swine influenza (H1N1) antibodies in porcine serum.
SUMMARY AND EXPLANATION
The influenza virus is responsible for swine flu, one of the most common diseases throughout the world. Swine flu is contagious and mainly characterized by coughing, anorexia, lethargy, and nasal discharge. Other characteristics, although seldom observed, include abortion for sows and death for piglets. This virus has several subtypes, the most common being H1N1 and H3N2. The differentiation between the subtypes is important since it will optimize vaccine selection.
CHARACTERISTICS
Blocking ELISA: Specific antigens to swine influenza (H1N1)
Results available within 3 hours
Tests per kit:
o 2 plates: 180 samples
o 5 plates: 450 samples
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Preparation of samples
2. Incubation of samples and controls 1 hour / 23°C
3. Successive washes
4. Incubation with monodonal antibody 15 min / 23°C
5. Successive washes
6. Incubation with conjugate 1 hour / 23°C
7. Successive washes
8. Revelation with substrate 10 min / 23°C
9. Stop the reaction
10. Reading optical densities 450 nm
IMPORTANT :
Requires a permit to release veterinary biologics from CFIA in Canada. This product is not licensed by CFIA and any claims made have not been substantiated by CFIA.
Requires a Research and Evaluation import permit from the USDA in the US. This product is not licensed by the USDA and any claims made have not been substantiated by the USDA.
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2014-04-30
SWINECHECK® H3N2
PRODUCT CODE: TRM-513
DESCRIPTION
This is an immunoenzymatic assay for the detection of swine influenza (H3N2) antibodies in porcine serum.
SUMMARY AND EXPLANATION
The influenza virus is responsible for swine flu, one of the most common diseases throughout the world. Swine flu is contagious and mainly characterized by coughing, anorexia, lethargy, and nasal discharge. Other characteristics, although seldom observed, include abortion for sows and death for piglets. This virus has several subtypes, the most common being H1N1 and H3N2. The differentiation between the subtypes is important since it will optimize vaccine selection.
CHARACTERISTICS
Indirect ELISA: Specific antigens to swine influenza (H3N2)
Rapid (less than 2 hours)
Tests per kit: 92 samples
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Preparation of samples
2. Incubation of samples and controls 30 min / 37°C
3. Successive washes
4. Incubation with conjugate 30 min / 37°C
5. Successive washes
6. Revelation with substrate 15 min / 23°C
7. Stop the reaction
8. Reading the optical densities 405 nm (reference filter at 490 nm)
IMPORTANT :
Requires a permit to release veterinary biologics from CFIA in Canada. This product is not licensed by CFIA and any claims made have not been substantiated by CFIA.
Requires a Research and Evaluation import permit from the USDA in the US. This product is not licensed by the USDA and any claims made have not been substantiated by the USDA.
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2015-04-30
SWINECHECK® HPS
PRODUCT CODE: TRM-521
DESCRIPTION
This is an immunoenzymatic assay for the detection of antibodies against Haemophilus parasuis (HPS) in porcine serum.
SUMMARY AND EXPLANATION
HPS is responsible for Glasser syndrome, a condition characterized by septicaemia which results in arthritis and meningitis. To date, 15 different serotypes have been identified with serotypes 1, 5, 10, 12, 13 and 14 being the most pathogenic.
CHARACTERISTICS
Indirect ELISA: Specific antigens to Haemophilus parasuis.
Rapid (less than 2 hours)
Test per kit: 92 samples tested as simplicate
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Preparation of samples
2. Incubation of samples and controls 30 min / 23°C
3. Successive washes
4. Incubation of conjugate 30 min / 23°C
5. Successive washes
6. Revelation with substrate 20 min / 23°C
7. Stop the reaction
8. Reading of optical densities 405 nm and reference filter at 490 nm
IMPORTANT :
Requires a permit to release veterinary biologics from CFIA in Canada. This product is not licensed by CFIA and any claims made have not been substantiated by CFIA.
Requires a Research and Evaluation import permit from the USDA in the US. This product is not licensed by the USDA and any claims made have not been substantiated by the USDA.
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2014-04-30
SWINECHECK® PCV2 indirect – 2 or 5 plates
PRODUCT CODE: TRM-559
DESCRIPTION
Swinecheck® PCV2 IgG indirect is an immunoenzymatic assay for the detection of IgG antibodies to porcine circovirus type 2 (PCV2) in porcine serum.
SUMMARY AND EXPLANATION
PCV2 has been associated to various clinical signs in weaned and growing pigs (the so-called Porcine Circovirus Associated Diseases, PCVAD), the most important being the Post-Weaning Multisystemic Wasting Syndrome (PMWS). Other frequent conditions associated with PCV2 include Porcine Dermatitis and Nephropathy Syndrome (PDNS), enteritis, myocarditis, and abortions or stillbirths.
Diagnosis of PCVAD relies on the demonstration of typical lesions associated with the presence of PCV2 nucleic acids or antigens. Measurement of antibodies (serology) may be helpful in evaluating virus exposure. It is also useful to evaluate the decrease of maternally derived antibodies and to implement efficient vaccination programs. Numerous serological assays have been developed to detect PCV2 antibodies (IFA, IPMA, ELISA, etc.). Enzyme-Linked Immuno Assays (ELISA) are the most popular as they are easy to perform and to be automatized.
CHARACTERISTICS
Test per kit: up to 184 samples
Recombinant antigen (PCV2 capsid)
Indirect quantitative ELISA
Results within two hours
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Incubation of the samples 60 minutes at 23°C ± 2°C
2. Serial washings
3. Incubation with the conjugate 30 minutes at 23°C ± 2°C
4. Serial washings
5. Incubation with the substrate 10 minutes at 23°C ± 2°C
6. Plate reading (450 nm)
7. Interpretation of the results
IMPORTANT :
Requires a permit to release veterinary biologics from CFIA in Canada. This product is not licensed by CFIA and any claims made have not been substantiated by CFIA.
Requires a Research and Evaluation import permit from the USDA in the US. This product is not licensed by the USDA and any claims made have not been substantiated by the USDA.
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2015-04-30
SWINECHECK® PED indirect – 2 or 5 plates
PRODUCT CODE: TRM-556/558
DESCRIPTION
This kit is based on an immunoenzymatic assay (ELISA) for the detection of antibodies to Porcine Epidemic Diarrhea Virus (PEDV) in porcine serum samples.
SUMMARY AND EXPLANATION
Porcine epidemic diarrhea (PED) is a swine disease characterized by acute diarrhea and vomiting. The disease is very contagious and affects pigs of all ages. The mortality may reach 100% in young piglets but is negligible in mature pigs.
PED is caused by a coronavirus distinct from the other porcine coronaviruses more especially the transmissible gastroenteritis virus (TGEV). PED was first reported in Europe in the late ’60 and is now prevalent in Asian South East and more recently in the USA.
PED diagnosis relies on the demonstration of PEDV nucleic acids or antigens in various specimens or of PEDV antibodies in serum. Several serological tests have been developed to detect PEDV antibodies. Enzyme-Linked Immuno Assays (ELISA) are easy to perform and to be automatized.
CHARACTERISTICS
Indirect ELISA: Specific antigens to PEDV
2 breakable plates/kit
Results available within 4 hours
Tests per kit:
o 2 plates: up to 184 samples
o 5 plates: up to 460 samples
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Preparation of samples
2. Incubation of samples and controls 1hr / 23°C
3. Successive washes
4. Incubation with conjugate A 1 hr / 23°C
5. Successive washes
6. Incubation with conjugate B 1 hr / 23°C
7. Successive washes
8. Revelation with substrate 10 min / 23°C
9. Stop the reaction
10. Reading optical densities 450 nm
IMPORTANT :
Requires a permit to release veterinary biologics from CFIA in Canada. This product is not licensed by CFIA and any claims made have not been substantiated by CFIA.
Requires a Research and Evaluation import permit from the USDA in the US. This product is not licensed by the USDA and any claims made have not been substantiated by the USDA.
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2015-04-30
SWINECHECK® PRRSV type 1 and 2 indirect
PRODUCT CODE: TRM-525
DESCRIPTION
Swinecheck® PRRSV type 1 and 2 indirect is an immunoenzymatic assay for the detection of antibodies to porcine reproductive and respiratory syndrome virus (PRRSV) type 1 and 2 strains in porcine serum or plasma samples (EDTA or heparin).
SUMMARY AND EXPLANATION
Porcine reproductive and respiratory syndrome virus (PRRS) is caused by the PRRSV which comprises two major genotypes: type 1 (“European”, EU) and type 2 (“North-American”, NA). The disease appeared in the late ’80 in Europe and North America and is now present in most swine producing countries. PRRS is characterized by reproductive problems such as increased abortion in late gestation, mummification and stillbirth as well as increased mortality rates and pneumonia in young pigs.
CHARACTERISTICS
Test per kit: up to 460 samples
Indirect ELISA
Detects antibodies to both type 1 and 2 of PRRSV
Results within less than two hours
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Incubation of the samples 30 minutes at 23°C + 2°C
2. Serial washings
3. Incubation with the conjugate 30 minutes at 23°C + 2°C
4. Serial washings
5. Incubation with the substrate 15 minutes at 23°C + 2°C
6. Plate reading (450 nm)
7. Interpretation of the results
IMPORTANT :
Requires a permit to release veterinary biologics from CFIA in Canada. This product is not licensed by CFIA and any claims made have not been substantiated by CFIA.
Requires a Research and Evaluation import permit from the USDA in the US. This product is not licensed by the USDA and any claims made have not been substantiated by the USDA.
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2015-04-30
SWINECHECK® TGEV recombinant – 2 or 5 plates
PRODUCT CODE: TRM-534/539
DESCRIPTION
This kit is based on an immunoenzymatic assay for the detection of antibodies against transmissible gastroenteritis virus (TGEV) in porcine serum.
SUMMARY AND EXPLANATION
Transmissible gastroenteritis (TGE) is a highly contagious enteric disease of swine caused by TGEV. TGEV affects swine of all ages. It multiplies in the digestive and respiratory tracts and causes vomiting and diarrhea. The mortality rate in piglets under 2 weeks of age is near 100%. In older pigs, the disease is milder and can even occasionally go unnoticed.
A fast and accurate diagnosis of TGE is essential to prevent dissemination of the disease. Diagnosis is mostly based on viral identification. However detection of antibodies is very useful in older pigs that do not always display typical signs of disease. Serological tests are also regularly required for commercial exchanges.
Antibodies against TGEV can be detected using the virus neutralization assay (VN). However, the VN test requires cell culture facilities and the use of infectious TGEV which limits its use to specialized laboratories.
Moreover, it is very time consuming and results are available after several days only. By contrast, enzyme-linked immunosorbent assays (ELISA) can be run in most laboratories and results are available within a few hours.
Use of monoclonal antibodies specific to TGEV in a blocking ELISA assay allows the detection of TGEV antibodies whereas antibodies directed against porcine respiratory coronavirus (PRCV), a closely related swine coronavirus, are not detected. The specificity of the assay is improved by using a recombinant protein as antigen.
CHARACTERISTICS
Blocking ELISA: Specific antigens to TGEV
Rapid (less than 2 hours)
Test per kit:
o 2 plates: 184
o 5 plates: 460
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Preparation of samples
2. Incubation of samples and controls 1h00 / 37°C
3. Successive washes
4. Incubation with conjugate 30 min / 37°C
5. Successive washes
6. Revelation with substrate 10 min / 23°C
7. Stop the reaction
8. Reading optical densities 450 nm
IMPORTANT :
CFIA-licensed.
Requires a Research and Evaluation import permit from the USDA in the US. This product is not licensed by the USDA and any claims made have not been substantiated by the USDA.
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2015-04-30
SWINECHECK® TGEV/PRCV recombinant – 2 or 5 plates
PRODUCT CODE: TRM-535/552
DESCRIPTION
This kit is based on an immunoenzymatic assay for the detection and differentiation of antibodies against transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV) in porcine serum.
SUMMARY AND EXPLANATION
Transmissible gastroenteritis (TGE) is a highly contagious enteric disease of swine caused by TGEV. TGEV affects swine of all ages. It multiplies in the digestive and respiratory tracts and causes vomiting and diarrhea. The mortality rate in piglets under 2 weeks of age is near 100%. In older pigs, the disease is milder and can even occasionally go unnoticed.
PRCV is a respiratory variant of TGEV which has lost the ability to colonize the digestive tract. PRCV infects swine of all ages and can cause benign respiratory infections. Its close relationship with TGEV complicates the diagnosis of TGE.
A fast and accurate diagnosis of TGE is essential to prevent dissemination of the disease. Diagnosis is mostly based on viral identification. However detection of antibodies is very useful in older pigs that do not always display typical signs of disease. Serological tests are also regularly required for commercial exchanges.
As antibodies against TGEV and PRCV show complete cross-reaction by neutralization, the differentiation of antibodies aimed against these two viruses is impossible by seroneutralization testing. However, the use of monoclonal antibodies in a competitive ELISA assay allows the detection and differentiation of infections caused by these two viruses. The specificity of the assay is improved by using a recombinant protein as antigen.
CHARACTERISTICS
Blocking ELISA: Specific antigens to TGEV
Rapid (less than 2 hours)
Test per kit:
o 2 plates: 88 samples tested for each virus
o 5 plates: 220 samples tested for each virus
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Preparation of samples
2. Incubation of samples and controls 1h00 / 37°C
3. Successive washes
4. Incubation with conjugate 30 min / 37°C
5. Successive washes
6. Revelation with substrate 10 min / 23°C
7. Stop the reaction
8. Reading optical densities 450 nm
IMPORTANT :
CFIA-licensed.
Requires a Research and Evaluation import permit from the USDA in the US. This product is not licensed by the USDA and any claims made have not been substantiated by the USDA.
Diagnostic kits
O V U C H E C K
( P r o g e s t e r o n e m e a s u r e m e n t )
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2015-04-30
OVUCHECK® Milk
PRODUCT CODE: TRM-547
DESCRIPTION
The OVUCHECK® MILK ELISA is an immunoenzymatic test which provides a simple, reliable and precise measurement of progesterone in whole milk from dairy cows. The concentration range covered by the reagents is 1 to 30 ng/mL. Each kit contains sufficient reagents for up to 92 tests plus four standards. OVUCHECK® MILK is used for oestrus detection and assessment of pregnancy status/luteal function in cows.
SUMMARY AND EXPLANATION
The OVUCHECK® MILK test is based on the competitive binding of unlabelled progesterone present in the standard or whole milk sample, and a fixed quantity of progesterone labeled with the enzyme alkaline phosphatase (AP), to binding sites on a limited amount of specific progesterone antibodies. The wells are pre-coated with antibodies, providing a solid phase for the convenient separation of the bound progesterone from the free progesterone in the milk. After incubation, all components other than those bound to the plate wells are washed away. The amount of bound AP-labelled progesterone remaining on the wells is inversely proportional to the concentration of the unlabelled progesterone present in the sample. The bound labelled progesterone is then measured by reacting the AP with its substrate during a second incubation. The colour produced is measured spectrophotometrically and the concentration of progesterone in the milk is determined from a standard curve. Alternatively, the colour can be interpreted visually.
CHARACTERISTICS
Qualitative test
Competitive binding of progesterone
Standard curve determined by progesterone standards (1, 5, 10 and 30 ng/ml)
Tests per kit: 92 possible tests
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Preparation of substrate
2. Incubation of samples, standards and conjugate 30 min / AT
3. Successive washes
4. Incubation with substrate 30 min / AT
5. Stop the reaction
6. Reading of the results 405 nm
AT: Ambiant Temperature
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2015-04-30
OVUCHECK® Plasma
PRODUCT CODE: TRM-546
DESCRIPTION
The OVUCHECK® PLASMA ELISA kit is an immunoenzymatic test which provides a simple, reliable and precise measurement of progesterone in plasma or serum of cows and bitches. Each kit con-tains sufficient reagents for up to 92 tests plus four standards. OVUCHECK® PLASMA is used for oestrus detection and assessment of pregnancy status and luteal function.
SUMMARY AND EXPLANATION
The OVUCHECK® PLASMA test is based on the competitive binding of unlabelled progesterone present in the standard or sample, and a fixed quantity of progesterone labelled with the enzyme alkaline phosphatase (AP) (conjugate), to binding sites on a limited amount of specific progester-one antibodies. The wells are pre-coated with antibodies, providing a solid phase for the capture of the progesterone present in the samples, the standards or the conjugate. After incubation, all components other than those bound to the plate wells are washed away. The amount of bound AP-labelled progesterone remaining on the wells is inversely proportional to the concentration of the unlabelled progesterone present in the sample. The bound labelled progesterone is then measured by making the AP react with its substrate during a second incubation. The colour pro-duced is measured spectrophotometrically and the concentration of progesterone in the sample is determined from a standard curve.
CHARACTERISTICS
Qualitative test
Competitive binding of progesterone
Standard curve determined by progesterone standards (1, 2.5, 5 and 10 ng/mL)
Tests per kit: 92 possible tests
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Preparation of substrate
2. Incubation of samples, standards and conjugate 30 min / AT
3. Successive washes
4. Incubation with substrate 30 min / AT
5. Stop the reaction
6. Reading of the results 405 nm
AT: Ambiant Temperature
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2015-04-30
OVUCHECK® Premate 5 and 10
PRODUCT CODE: TRM-540-541
DESCRIPTION
OVUCHECK® PREMATE is a semi-quantitative test for the measurement of progesterone in a drop of plasma or serum of the bitch. The quantity of progesterone present is indicated by a change in colour, which is compared with high and low progesterone standards. This kit is quick and simple to use. It gives reliable information which allows you to:
- plan mating at the optimum time - determine the time of whelping - investigate the causes of infertility (anovular cycles, silent heats with normal
ovulation)
SUMMARY AND EXPLANATION
Hormonal changes occurring in the bitch in the course of pro-oestrus, oestrus, beginning of dioestrus, and gestation are described below.
At the end of pro-oestrus, the drop in oestrogen levels, secreted by the maturing ovarian follicle, causes the luteinising hormone (LH) surge. Ovulation normally happens 24 to 48 hours after the LH surge.
The follicle gradually produces progesterone above basal levels (<0.5 ng/mL) a few days before the LH surge. When the LH surge occurs, progesterone levels may reach 2 to 4 ng/mL.
At the time of ovulation, the progesterone level is generally between 4 and 10 ng/mL. This normally happens 11 to 13 days after the start of pro-oestrus (characterized by the presence of blood and swelling of the vulva).
After ovulation, progesterone levels continue to rise to reach maximal levels 2 to 3 weeks after the start of dioestrus. However, there are large variations between breeds and individuals. The determination of progesterone level with OVUCHECK® PREMATE in association with other elements such as the behaviour of the dog and vaginal cytology allows determination of the time of ovulation and therefore the optimal time for mating.
At the end of gestation, 12 to 24 hours before giving birth, the level of progesterone falls again to reach values of less than 2 ng/mL. Thus, a level of progesterone higher than the low standard of the OVUCHECK® PREMATE test shows that parturition will not occur in the next 12 to 24 hours.
CHARACTERISTICS
Semi-quantitative test
Competitive binding of progesterone
Rapid (less than 1 hour)
Tests per kit: 5 tests Premate 5
10 tests Premate 10
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Preparation of substrate
2. Incubation of samples, standards and conjugate 15 min / AT
3. Successive washes
4. Incubation with substrate 15 min / AT
5. Interpretation of the results
AT: Ambiant Temperature
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2015-04-30
OVUCHECK® Premate porcine
PRODUCT CODE: TRM-526
DESCRIPTION
OVUCHECK® PREMATE PORCINE is an immunoenzymatic (ELISA) kit which provides a simple and reliable measurement of progesterone in plasma or serum of female swine (gilts, sows). OVUCHECK® PREMATE PORCINE is intended for assessment of ovarian function and provides a reliable aid to sow reproduction management. Each kit contains sufficient reagents for up to 28 tests.
SUMMARY AND EXPLANATION
The OVUCHECK® PREMATE PORCINE test is based on the competitive binding of unlabelled progesterone present in the standard or sample, and a fixed quantity of progesterone labelled with the enzyme alkaline phosphatase (AP) (conjugate), to binding sites on a limited amount of specific progesterone antibodies.
The wells are pre-coated with specific progesterone antibodies, providing a solid phase for the capture of the progesterone present in the samples, the standards or the conjugate. After incubation, all components other than those bound to the plate wells are washed away. The amount of bound AP-labelled progesterone remaining on the wells is inversely proportional to the concentration of the unlabelled progesterone present in the sample. The bound labelled progesterone is then measured by making the AP react with its substrate during a second incubation. The colour obtained with the sample is compared to that produced by two standards of 2.5 and 5.0 ng of progesterone/mL.
CHARACTERISTICS
Competitve ELISA
Semi-quantitative test
2 progesterone standards (2.5 et 5.0 ng/mL)
Tests per kit: 28
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2015-04-30
OVUCHECK® Rapid Well
PRODUCT CODE: TRM-548
DESCRIPTION
OVUCHECK® RAPID WELL is used to measure the level of progesterone in a drop of cow’s milk. The quantity of progesterone present is indicated by a change in colour, which is compared with a standard. OVUCHECK® RAPID WELL is a simple and rapid test which provides a reliable aid to cow fertility management.
SUMMARY AND EXPLANATION
The amount of progesterone is almost zero at oestrus (heat) and then increases and remains at a high level. The progesterone level falls suddenly 17 to 20 days after the last heat, indicating the onset of the next cycle. This fall does not occur if the animal is pregnant.
CHARACTERISTICS
Qualitative test
Rapid (Less than 30 minutes)
Competitive binding of progesterone
Tests per kit: 31 possible tests
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Incubation of samples, standard and conjugate 5 min / AT
2. Successive washes
3. Incubation of substrate B and C 5 min / AT
4. Interpretation of the results
AT: Ambiant Temperature
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2015-04-30
OVUCHECK® Reader
PRODUCT CODE: TRD-751
DESCRIPTION
OVUCHECK® Reader is a Convertible Well-strip/Cuvette Reader and is a portable miniphotometer designed to do ELISA testings in either 1x8/1x12 microwell strips or round/square cuvettes by interference filter for wavelength of 400-700nm.
OVUCHECK® Reader is a user-friendly ELISA reader. All operations are carried out with only two touch buttons by following instruction on the LCD display, and results can be obtained within two seconds.
OVUCHECK® Reader undertakes testings with an extreme economic Reagent consumption, in microwell at 0.3ml and in cuvette at 0.5ml.
SUMMARY AND EXPLANATION
OVUCHECK READER® can be use with Biovet Ovucheck® kits to measure progesterone levels in milk or blood.
CHARACTERISTICS
portable, compact, accurate, easy to use and affordable
Holders for 12mm diameter tubes or 9 x 13mm well strips.
Houses multiple filters, including the 405 nm and 550 nm filters
Changing filters and holders is fast and very easy.
Instructions that are as simple as using a video device.
Measurement accuracy comparable to more expensive devices.
Diagnostic kits
OTHE R S
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2015-04-30
Dermatophytes
PRODUCT CODE: TRM-560
DESCRIPTION
This is a culture medium for rapid isolation and identification of dermatophytes.
SUMMARY AND EXPLANATION
In order to directly apply this method on samples taken in surgery or in the field, this kit is easy to use, giving the possibility of having a diagnosis in a few days. It contains specific nutrients, which facilitate the growth of dermatophytes and selective antibiotics which prevent the growth of non-pathogenic saprophytic mycetes and bacteria. The interpretation relies on the pH color indicator, which immediately shows the presence of dermatophytes by a color change, because Microsporum, Trichophyton and Epidermophyton produce alkaline metabolites.
CHARACTERISTICS
Rapid isolation and identification of dermatophytes.
Color change: 24-72 hours in presence of dermatophytes
Tests per kit: 10 tests / package
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Carry out the sampling (hair, skin, scab, etc)
2. Place the sample on the edge of the culture medium.
3. Incubate at room temperature 48 hours / 23°C
4. Leave the sample in position for about 10 to 14 days to identify the colonies.
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2015-04-30
Bio-Tubes® - Blood sample test kit
PRODUCT CODE: TRM-531
MATERIALS PROVIDED
100 Bio-Tubes® with screw caps.
MATERIALS REQUIRED BUT NOT PROVIDED
100 hypodermic needles 18G 1 ½ (single use)
Rubber bands
Rubbing alcohol
Gauze
Permanent marker
Stickers
PROCEDURE
1. Apply a rubber band at the base of the ear like a tourniquet
2. Rub the outside of the ear with alcohol
3. Allow to air dry
4. Attach the needle to the hub of the Bio-Tube
5. Gently insert the needle into the largest vein of the ear
6. Let the blood flow into the tube by gravity (can be harvested in a 1.5 ml)
7. Carefully remove the needle from the vein
8. Remove the rubber band at the base of the ear
9. Apply pressure on the puncture site to start clotting
10. Remove the needle from the hub of the Bio-Tube
11. Dispose of the needle appropriately
12. Unscrew the Bio-Tube and remove the hub with the pipe
13. Close the Bio-Tube with a screw cap
14. Identify the Bio-Tube with a permanent marker or a sticker
15. Let the Bio-Tube clot at room temperature for 30-45 minutes
16. Place the Bio-Tube in a Ziploc® bag
17. Bio-Tubes can be refrigerated before shipping to the laboratory
18. Complete a request form
19. Place the Ziploc® bag inside another Ziploc® bag with the request
20. Send to the laboratory in a cooler with ice packs
Diagnostic kits
Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996
www.biovet.ca
2015-04-30
Sampling Kit for collection of oral fluids
PRODUCT CODE: TRM-530
MATERIALS PROVIDED
cotton rope (about 30 feet)
10 tubes 50 ml
10 pairs of vinyl gloves
10 small plastic bags
10 medium plastic bags
MATERIALS REQUIRED BUT NOT PROVIDED
1 pair of scissors
Permanent marker
PROCEDURE
1. Fold the cotton rope in half and fix tightly in an easily accessible place for the animals
2. The free ends of the rope should be at shoulder height of the animals in no way contacting the ground.
3. Leave the rope in place for about thirty minutes
4. When the rope is sufficiently soaked, untie it
5. Place the tip that was chewed by animals in the plastic bag
6. Twist it with your hands to express the oral fluids into the plastic bag
7. Remove the rope
8. Cut one corner of the plastic bag
9. Pour the oral fluids into the Falcon tube
o The laboratory requires a minimum 3 ml of fluid
10. Close the Falcon tube tightly
11. Identify the tube with a permanent marker or a sticker
12. Place it in a plastic bag
13. Immediately put the bag in a cooler on ice packs
14. If samples cannot reach the laboratory the same day, freeze them (-20°)
15. Complete a request form
16. Place the plastic bag inside another bag with the request
17. Send that to the laboratory in a cooler with ice packs
ASSAY PROCEDURE INCUBATION TIME/ TEMP.
1. Preparation of substrate
2. Addition of samples, standards and conjugate and incubation
15 min/RT
3. Washings
4. Addition of substrate and incubation 15 min/RT
5. Stopping of the enzymatic reaction
6. Revelation with substrate
7. Stopping of the enzymatic reaction
8. Comparison of sample and standard colorations
RT: Room Temperature
See reverse for Sampling kits and diagnostic Services
Sampling Kits Bio-Tubes® - Blood sampling kit (PRRS) Collection of oral fluids (Influenza, PCV2, PRRS, PEDV)
Biovet is a biotechnology company providing unique expertise in the field of diagnostics for
animal health and agro-industry specialists. Biovet operates a USDA and CFIA certified* laboratory offering a complete array of innovative diagnostic services for veterinarians. Biovet employs more than 50 people including 15 scientists.
Two laboratories in North America to meet your diagnostic needs.
Canada Biovet Canada 4375, Beaudry avenue Saint-Hyacinthe, QC J2S 8W2 Canada Tel.: 450-771-7291 Fax: 450-771-4158 Toll free : 888-824-6838 [email protected]
USA Biovet USA 9025 Penn Avenue South Bloomington, MN 55431 USA Tel.: 952-884-3113 Fax: 952-884-3473 Toll free : 877-824-6838 [email protected]
888 824-6838 [email protected]