biotek elx 808 – gen5 software screenshots technochrom … · 3/11 . fill in as shown in the...
TRANSCRIPT
![Page 1: BIOTEK ELx 808 – Gen5 Software Screenshots TECHNOCHROM … · 3/11 . Fill in as shown in the screenshot above and confirm settings with “OK” . Kinetic steps are defined as](https://reader035.vdocuments.us/reader035/viewer/2022070719/5edec97bad6a402d666a2360/html5/thumbnails/1.jpg)
1/11
BIOTEK ELx 808 – Gen5 Software Screenshots
TECHNOCHROM® FXIII
Table of contents 1 How to create a protocol with factor calculation ................................................................................. 2
1.1 Starting the software...................................................................................................................... 2
1.2 Procedure settings ......................................................................................................................... 2
1.3 Plate Layout settings ..................................................................................................................... 4 1.4 Data Reduction settings ................................................................................................................. 5
1.4.1 Define Well Analysis as shown below .................................................................................. 6
1.4.2 Define Transformations ......................................................................................................... 7
1.5 Report/Export settings ................................................................................................................... 9
In accordance with good clinical laboratory practices, your laboratory must validate this
instrument application prior to routine use.
ML-00-00383Rev01_FXIII F Factor Calculation_Screenshots_BIOTEK ELx808 Gen 5_FXIII
![Page 2: BIOTEK ELx 808 – Gen5 Software Screenshots TECHNOCHROM … · 3/11 . Fill in as shown in the screenshot above and confirm settings with “OK” . Kinetic steps are defined as](https://reader035.vdocuments.us/reader035/viewer/2022070719/5edec97bad6a402d666a2360/html5/thumbnails/2.jpg)
2/11
1 How to create a protocol with factor calculation
1.1 Starting the software
Double-click on the software icon on the desktop
Click on “Create a New Item”, “Protocol”.
1.2 Procedure settings
Double-click on “procedure” to set up a new protocol.
ML-00-00383Rev01_FXIII F Factor Calculation_Screenshots_BIOTEK ELx808 Gen 5_FXIII
![Page 3: BIOTEK ELx 808 – Gen5 Software Screenshots TECHNOCHROM … · 3/11 . Fill in as shown in the screenshot above and confirm settings with “OK” . Kinetic steps are defined as](https://reader035.vdocuments.us/reader035/viewer/2022070719/5edec97bad6a402d666a2360/html5/thumbnails/3.jpg)
3/11
Fill in as shown in the screenshot above and confirm settings with “OK”
Kinetic steps are defined as
Confirm settings with “OK”.
ML-00-00383Rev01_FXIII F Factor Calculation_Screenshots_BIOTEK ELx808 Gen 5_FXIII
![Page 4: BIOTEK ELx 808 – Gen5 Software Screenshots TECHNOCHROM … · 3/11 . Fill in as shown in the screenshot above and confirm settings with “OK” . Kinetic steps are defined as](https://reader035.vdocuments.us/reader035/viewer/2022070719/5edec97bad6a402d666a2360/html5/thumbnails/4.jpg)
4/11
Add calibrator 1 and Calibrator 0 (Blank), controls and samples.
Fill in the Layout as shown in the picture above. (Sample ID can be chosen individually!). Confirm settings with “OK”
1.3 Plate Layout settings
Note: The example below utilizes a STD & STD blank for the factor (F) calculation. The controls & samples do not have blanks as they were not deemed as necessary for this laboratory. Contact your BioTek representative to incorporate control & sample blank values into the reader analysis.
Double-click on “Plate Layout” to set up the plate layout.
ML-00-00383Rev01_FXIII F Factor Calculation_Screenshots_BIOTEK ELx808 Gen 5_FXIII
![Page 5: BIOTEK ELx 808 – Gen5 Software Screenshots TECHNOCHROM … · 3/11 . Fill in as shown in the screenshot above and confirm settings with “OK” . Kinetic steps are defined as](https://reader035.vdocuments.us/reader035/viewer/2022070719/5edec97bad6a402d666a2360/html5/thumbnails/5.jpg)
5/11
Attention!!! Calibrator value is lot dependent and concentration hase to be entered for each lot.
1.4 Data Reduction settings
Double-click on “Data Reduction” to set up the data reduction.
ML-00-00383Rev01_FXIII F Factor Calculation_Screenshots_BIOTEK ELx808 Gen 5_FXIII
![Page 6: BIOTEK ELx 808 – Gen5 Software Screenshots TECHNOCHROM … · 3/11 . Fill in as shown in the screenshot above and confirm settings with “OK” . Kinetic steps are defined as](https://reader035.vdocuments.us/reader035/viewer/2022070719/5edec97bad6a402d666a2360/html5/thumbnails/6.jpg)
6/11
1.4.1 Define Well Analysis as shown below
Kinetic 1
Confirm settings with “OK”
Kinetic 1
Confirm settings with “OK”
ML-00-00383Rev01_FXIII F Factor Calculation_Screenshots_BIOTEK ELx808 Gen 5_FXIII
![Page 7: BIOTEK ELx 808 – Gen5 Software Screenshots TECHNOCHROM … · 3/11 . Fill in as shown in the screenshot above and confirm settings with “OK” . Kinetic steps are defined as](https://reader035.vdocuments.us/reader035/viewer/2022070719/5edec97bad6a402d666a2360/html5/thumbnails/7.jpg)
7/11
1.4.2 Define Transformations
Custom SDT1-STD2
Ratio
ML-00-00383Rev01_FXIII F Factor Calculation_Screenshots_BIOTEK ELx808 Gen 5_FXIII
![Page 8: BIOTEK ELx 808 – Gen5 Software Screenshots TECHNOCHROM … · 3/11 . Fill in as shown in the screenshot above and confirm settings with “OK” . Kinetic steps are defined as](https://reader035.vdocuments.us/reader035/viewer/2022070719/5edec97bad6a402d666a2360/html5/thumbnails/8.jpg)
8/11
Custom Concentration
Confirm settings with “OK”
Filled in “Data Reduction” is
ML-00-00383Rev01_FXIII F Factor Calculation_Screenshots_BIOTEK ELx808 Gen 5_FXIII
![Page 9: BIOTEK ELx 808 – Gen5 Software Screenshots TECHNOCHROM … · 3/11 . Fill in as shown in the screenshot above and confirm settings with “OK” . Kinetic steps are defined as](https://reader035.vdocuments.us/reader035/viewer/2022070719/5edec97bad6a402d666a2360/html5/thumbnails/9.jpg)
9/11
1.5 Report/Export settings
Double-click on “Report/Export Builder” to set up the report and export.
Attention!!! These settings can be individually adapted
Double-click on “New Report” to set up the report
ML-00-00383Rev01_FXIII F Factor Calculation_Screenshots_BIOTEK ELx808 Gen 5_FXIII
![Page 10: BIOTEK ELx 808 – Gen5 Software Screenshots TECHNOCHROM … · 3/11 . Fill in as shown in the screenshot above and confirm settings with “OK” . Kinetic steps are defined as](https://reader035.vdocuments.us/reader035/viewer/2022070719/5edec97bad6a402d666a2360/html5/thumbnails/10.jpg)
10/11
Example of report settings
ML-00-00383Rev01_FXIII F Factor Calculation_Screenshots_BIOTEK ELx808 Gen 5_FXIII
![Page 11: BIOTEK ELx 808 – Gen5 Software Screenshots TECHNOCHROM … · 3/11 . Fill in as shown in the screenshot above and confirm settings with “OK” . Kinetic steps are defined as](https://reader035.vdocuments.us/reader035/viewer/2022070719/5edec97bad6a402d666a2360/html5/thumbnails/11.jpg)
11/11
Confirm settings with “OK”
Double-click on “New Export to text” to set up the export
Define the export content
Confirm settings with “OK”
ML-00-00383Rev01_FXIII F Factor Calculation_Screenshots_BIOTEK ELx808 Gen 5_FXIII