biosynthesis of silver and gold nanoparticles using microbial biomass
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Department of Neutron Activation Analysis Division of Nuclear Physics Frank Laboratory of Neutron Physics Joint Institute for Nuclear Research. Biosynthesis of silver and gold nanoparticles using microbial biomass. Inga Zinicovscaia E-mail: [email protected]. - PowerPoint PPT PresentationTRANSCRIPT
Biosynthesis of silver and gold nanoparticles using microbial biomass
Inga Zinicovscaia
E-mail: [email protected]
Department of Neutron Activation Analysis Division of Nuclear Physics
Frank Laboratory of Neutron PhysicsJoint Institute for Nuclear Research
JINR Scientific Council, September 15-16, 2011
M.V. Frontasyeva, S.S. Pavlov
Frank Laboratory of Neutron Physics , JINR, Russian Federation
T. Kalabegishvili , E. Kirkesali , I. Murusidze ,
D. Pataraya, E.N. Ginturi
Andronikashvili Institute of Physics, Tbilisi , Georgia
I. Zinicovscaia, Gh. Duca
Institute of Chemistry of the Academy of Science of Moldova, Chisinau, Moldova
An important area of research in nanotechnology deals with synthesis of nanoparticles of different chemical composition and size. There is a growing need to develop environmentally gentle nanoparticle synthesis that does not use toxic chemicals in its process.
As a result, researchers in the field of nanoparticle synthesis have turned to biological systems. It is well known that many organisms, both uni-cellular and multi-cellular, are producing inorganic materials either intra- or extra-cellularly.
Introduction
3
Advantages of biological method
tightly controlled, highly reproducible
syntheses
biocompatible particles
the avoidance of toxic surfactants or organic solvents
4
Biotechnology of Biotechnology of
silver nanoparticlessilver nanoparticles
Application of silver nanoparticles
nonlinear optics
medicine
electronics
catalysis
microelectronics
6
UV-Vis spectra recorded after one week for the reaction mixture prepared using 1mM silver nitrate and 1 g
Streptomyces glaucus 71MD
+ AgNO3 1mM =
Experiment
yellowish brown pale yellow
7
Quanta 3D FEG
Resolution 1.2 nm
Magnification 5000–150000x
Voltage 1–30 kV
Scanning electron microscope
The Netherlands’ Firm “Systems for Microscopy and Analysis” (Moscow, Russia)
8
Objects of study
BacteriaStreptomyces glaucus 71MD
p
Blue-green microalga Spirulina platensis
9
Control
SEM micrographs of Spirulina platensis cells with silver nanoparticles
1 day
27 nm
10
C O
EDAX spectrum recorded from Spirulina platensis cells after formation of silver nanoparticles 11
Control
SEM micrographs of Streptomyces glaucus 71MD cells with silver nanoparticles
12 000x50 000x150 000x
12
EDAX spectrum of Streptomyces glaucus 71MD cells after exposure to silver nitrate solution
13
Biotechnology of Biotechnology of gold nanoparticlesgold nanoparticles
Application of gold nanoparticles
Catalysis
Chemical sensing
Biosensing
Medicine
15
Experiment
+ =HAuCl4
yellow red purple
Experiment I CHAuCl4 = 1mM
Incubation time: 1 − 6d
Experiment II Incubation time = constant CHAuCl4: 10-4 − 10-2M
16
UV-Vis spectra recorded after one week for the reaction mixture prepared using 1mM hydrated gold chloride and
1 g biomass of A. globiformis 151B
17
Control1 day2 days3 days5 days6 days
SEM micrographs of Spirulina platensis cells with gold nanoparticles at different incubation time
Experiment I
18
HAuCl410-4M10-3M5·10-3M
10-2M
SEM micrographs of Spirulina platensis cells with gold nanoparticles at different concentrations
Experiment II
19
EDAX spectrum of Sp. platensis cells after exposure to hydrated gold chloride solution
20
Conclusions
1. Production of silver and gold nanoparticles by blue-green microalgae Spirulina platensis and bacteria Streptomyces glaucus 71MD proceeds extra-cellularly
2. SEM and EDAX were used to characterize the silver and gold nanoparticles. SEM showed formation of nanoparticles in the range of:
a) 4–25 nm for Streptomyces glaucus 71MDb) 16–200 nm for Spirulina platensis (first
experiment)c) 15 nm–7 μm for Spirulina platensis (second
experiment)
21
For quantitative analysis of samples the
epithermal neutron activation analysis
(ENAA) in the radioanalytical complex
REGATA at the reactor IBR-2M will be
carried out by the end of 2011
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Elemental concentration in biomass of Streptomyces glaucus (irradiation time 8 s)
Element Energy, keV Concentration, µg/g Error, %
Ag 657.76 37 5
K 1524.58 3290 8
La 1596.21 15 14
Mn 846.75 25.0 6
Na 1368.55 381 5
Sb 564.24 1.3 15
The data were obtained by Sergey Pavlov (FLNP JINR) and Arnaud Faanhof (NECSA) in June 2011 at the reactor SAFARI-I
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Thank you for attention!Thank you for attention!