biosensor-lau weng loon

7/29/2019 Biosensor-Lau Weng Loon

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FACULTY OF ENGINEERING AND

BUILT ENVIRONMENT (FKAB)

KKKC 4006: TEKNOLOGI PERANTI

TERMAJU

NAME LAU WENG LOON

MATRICES NO. A127573

DEPARTMENT JKEES

ASSIGNMENT BIOSENSOR AND ITS

SPECIFICATIONSSEMESTER/SESSION 1 / 2012/2013

LECTURER Dr. NAZRUL ANUAR BIN

NAYAN

DUE DATE 2th NOVEMBER 2012


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Biosensor

A definition of biosensor can be depicted as an analytical device merging a biorecognition

system such as DNA, enzymes, antibodies, microorganisms that is either closely connected to,

or integrated within, a physic-chemical transducer for instances, electrochemical, optical,

piezoelectric or thermometric according to the mode of signal transducer (Shew et al. 2011) .

Biosensor also used transducer in order to convert the biologicalrecognition step into signal

which able interfacing to a microprocessor for sake of control and measurement. (Eggins et al.

1996 & Iqbal et al. 2000). The biosensor able reacts to the existence of a specific analyte by

causing an electric signal exist which is proportional to the concentration of the analyte. Mass

density, dielectric permittivity, or several other physical quantity are varied by the

accumulation of analyte on the biosensor that can be converted to a measurable output signal

(Cooper et al. 2003). By the way, a biosensor constituent several bioactive components such

as an enzyme, an antibody, a cell, a tissue slice, a receptor or nucleic acid (Maria & Toby et

al. 2011). The consequence signal is produced by the complex interaction relating the

chemical equilibrium between an immobilized capture ligand and the traced analyte in which

both attachment and detachment occur simultaneously. On top of that, The ability of the

catalytic action of an enzyme or the affinity of antibodies or receptor proteins to modify the

electrical impedance of a suitably configured set of electrodes to determine the operation ofconductimetric biosensor (Norman et al. 1995). Mass transfer also able affect sensor output

signal and it may results in limitation of the rate of delivery of anayte molecules to surface.

(Choi et al. 2010). Besides that, biosensors also have been widely used for biological systems

for medicine and pharmaceutical industry such as drug monitoring and antibody

characterization. Moreover, biosensors practices able to ensure that the possibility of high

throughput analyses as well as the trace of multiple serovars or serogroups within a family of

infectious disease agents or antibodies against these agents in a effective manner. Apart from

that, there were several applications of biosensors such as: optical biosensor able to determine

very low levels of residues of veterinary drugs and growth hormones (Sternesjo et al. 1994),

magnetic nanoparticle biosensor exhibits excellent stability and high sensitivity of detection

with glucose (Wided et al. 2012)


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References

1. Cooper M.A.. 2003. Label-free screening of bio-molecular interactions.

Anal.Bioanal.Chem.377: 834842.

2. Shew B.Y., Kuo C.H., Huang Y.C. & Tsai Y.H.. 2005. UV-LIGA interferometer

biosensor based on the SU-8 optical waveguide. Sensors and Actuators A 120: 383389.

3. Maria N. VelascoGarcia &Toby Mottram. 2001. Biosensors in the livestock industry: an

automated ovulation prediction system for dairy cows. TRENDS in Biotechnology 19: 433

434

4. Ase Sternesjo, Catarina Mellgren, & Lennart Bjorck. 1994. Determination of

Sulfamethazine Residues in Milk by a Surface Plasmon Resonance-Based Biosensor Assay.

Analytical Biochemistry 226: 175- 181.

5. Choi Charles J., Belobraydich Alysia R., Chan Leo L., Mathias Patrick C. & Cunningham

Brian T.. 2010. Comparison of label-free biosensing in microplate, microfluidic, and spot-

based affinity capture assays.Analytical Biochemistry 405: 1-10.

6. Sheppard Norman F., Jr & Mears David J.. 1996. Model of an immobilized enzyme

conductimetric urea biosensor.Biosensors & Bioelectronics 11: 967979.

7. B.R. Eggins,Biosensors: An Introduction, Wiley, New York, 1996, pp.1117.

8. Iqbal S.S., Mayo M.W., Bruno J.G., Bronk B.V., Batt C.A. & J.P.

Chambers. 2000. A review of molecular recognition technologies for detection of biological

threat agents.Biosens. Bioelectron. 15: 549578.

9. Wided Nouira, Abderrazak Maaref, Hamid Elaissari, Francis Vocanson, Maryam Siadat &

Nicole Jaffrezic-Renault. 2012. Comparative study of conductometric glucose biosensor

based on gold and on magnetic nanoparticles. Materials Science and Engineering C33 (1):

298303.


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biosensor-lau weng loon

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