bioprocessing media and buffers product catalog

Bioprocessing Media and BuffersProduct Catalog

– For cell line development and media selection k Standard off-the-shelf media High quality | high performance serum-free formulation for all cell lines in bottles and small bags

– For media optimization k Customized media optimization service and support R&D specialists can optimize or fully develop new custom media engineered to your unique cell culture specifications

– For process development k Fast and clean custom production An expedited production option for those that require high quality cell culture media for process optimization or process development as well as quick buffer delivery

– For production k Custom media production under cGMP Protocols Small to large batch production following Current Good Manu-facturing Practices (cGMP 21CFR820) in ISO certified facilities Manufacturing practices compliant with standard regulations

Complete Solution

Media | Buffer Preparation Upstream Process

Pre-Viral Downstream Process

Post-Viral Downstream Process

LevMixer

Protein A Chromatography

FlexAct®

Biostat® B, RM, UniVesselFlexAct®

Low-pH Virus Inactivation

UF | DF

Sterile Filtration

CEX Chromatography

Storage

Storage

Storage

Wash | Equilibration | Elution buffers

Media

pH Adjustments buffers

Media and Feeds

Flush | Equilibration buffers

Dilution | Stabilization buffers

Processing Path Showing USP | DSP Elements

Wash | Equilibration | Elution buffers

Pre-Clinical

Wash | Flush | tize | Diafiltration buffers

A Media Solution to Fit Your Cell Line, Schedule and Budget

Features Benefits

Media and buffers in Sartorius Stedim bags kSame material for single use bioreactor bags to minimize the risk of leachables

Full media qualification package and support. All liquid media filtered through Sartorius filters

kOptimized media filtration and guaranteed successful scale-up thanks to complete support provided

Preweighed powder buffer and media in dispensing bags k Simple, ready-to-use media and buffers fully compatible with mixing technology

Integrated media feed strategies and PAT automation k Fully monitored and controlled cell culture conditionsProducts fully integrated into the Sartorius upstream and downstream solutions

kOne-stop supplier gives you full support so you can focus on your core tasks

Complete Solution

All the equipment and solutions you may need for your media and buffer preparation steps can also be provided by Sartorius, please refer to Section Related Products page 100-108.

Sterile Filtration

Biostat STR®

Polishing

FlexAct®

Storage

Depth Filtration

FlexAct®

Polishing

Virus Filtration

Storage

Storage

Filling Freezing

Media and Feeds

Flush | Equilibration buffers

Dilution | Stabilization buffers

Buffer Flush buffers

Cell Growth Media Buffers

Wash buffers Wash | Equilibration | Elution buffers

Glossary for Pictograms | Product FeaturesThe following pictograms indicate the available features of our products presented in a format that allows you to easily identify the information that matters to you most.

Animal Origin: formulation contains animal or human origin components

Non Animal Origin: the formulation is entirely made from non-animal and non-human origin components

Chemically Defined: The exactconcentration and size of everycomponent is known

For Further Manufacturing Use

Research Use Only

Product available in liquid format

Product available in powder format

Protein-free*: the formulation does not contain any animal-derived proteins

As part of our high manufacturing standards, all the products we supply in liquid format have been sterile filtered through Sartorius and made according to cGMP (21CFR820) conditions at ISO facilities.

* human recombinant insulin is NOT considered a protein, both because it is fully characterized, and because of its size (<5kDa) per Lonza definition this means that it does not include any proteins/peptides >10 kDa.

Bioprocessing Media and Buffers

Table of Contents

Upstream ApplicationsMulti Purpose Media p.14

Ultra Culture™ p.14

Insect-Xpress™ p.15

Recombinant Proteins p.17

PowerCHO™ Protein Expression Media p.18

PowerCHO™ Advance™ p.20

ProCHO™ p.22

Feeds p.23

GS-CHO p.24

CHO Media – The Optimization Tool Box p.26

CHOptimizer – The Media Builder Box p.28

ProNSO™ p.30

UltraDoma™ p.32

UltraDOMA-PF™ p.33

ProDoma™ p.34

Viral Vaccines p.35

ProMDCK™ p.36

UltraMDCK™ p.37

ProVero™ 1 p.38

ProPer™ 1 p.39

PERMEXCIS® p.40

Pro293™ p.42

Regenerative Medicine p.45

X-Vivo™ p.46

L7™ hPSC Culture System p.48

FGM-CD™ (Fibroblast Growth Media) p.50

KGM-CD™ p.51

MSCGM-CD™ p.52

Bioprocessing Media and Buffers

Downstream StepsThe Downstream Buffer Offering p.54

Buffer Packaging and Options p.56

DSP Buffers p.57

PBS p.58

DPBS p.59

Hanks p.60

Hepes p.60

Earle’s p.61

Other Buffers p.61

WFI p.62

ReagentsSodium bicarbonate p.64

Sodium pyruvate p.64

Insulin p.65

Trypsin p.65

L-Glutamine p.66

Ultra Glutamine p.66

Penicillin p.67

Amphotericin p.68

Gentamycin p.68

ProFreeze™ p.69

Anticlumping Agents p.70

ServicesCustomization p.74

Support p.74

Validation p.74

Quality p.76

Packaging and Shipping p.80

Introduction p.8

Flexibility p.10

Custom Production p.11

Table of Contents

Related ProductsSolutions for Media Preparation p.100

Sartopore® 2 XL Filter Family p.102

Sartogard PreFilter Familyy p.103

Virosart® p.104

UVivatec® p.105

Flexel® for Mixing1 p.106

FlexAct® MP p.107

Which Bioreactor Fits your Needs? p.108

Classical MediaBME (Basal Medium Eagle) p.84

DMEM (Dulbecco’s Modified Eagle’s Medium) p.84

EMEM (Eagle Minimum Essential Medium) p.86

DMEM:F12 Medium p.88

GMEM (Glasgow Minimum Essential Medium) p.88

Grace’s Insect p.89

Ham’s p.89

IMDM (Iscove’s Modified Dulbecco’s Medium) p.90

L-15 (Leibovitz) p.90

Mc Coy’s p.91

M199 p.91

PC-1™ p.92

HL-1™ p.94

RPMI p.96

NCTC-109 p.97

William’s E p.97

Richter’s p.97

UltraMEM™ p.98

8

In January 2013, Sartorius Stedim Biotech and Lonza joined forces to supply high quality, innovative cell culture media and buffers as well as complementary single-use solutions for biopharmaceutical, vaccine and cell therapy development and production.

Lonza-Sartorius offers a wide range of liquid and powder media products taking advantage of their respective expertise to help you scale-up your project and optimize your processes thanks to our leading position in single-use applications such as fluid management, filtration and process analytical technology.

Lonza-Sartorius is a pioneer in state-of-the-art micronization and high-quality powder production and is ISO-certified with a successful audit track record following current Good Manufacturing Practices 21CFR820.

Introduction

Florenz Sartorius mechanical workshop is founded

Sartorius starts commercial-scale manufacturing of membrane filters

Sartorius is founded as the original manufacturer of EVA plastic bags for total parental nutrition

1870 1922 1990 19941948 1970 1974 1979

Founded Media Production starts

Grand opening of the Walkersville facility in Maryland, USA

Initial manufacture of liquid media in Verviers, Belgium, EU

Stedim creates the market for bag systems used in biopharmaceutical manufacturing processes

9

Introduction

1999 2000 20142007 2008

Acquisition of Cambrex Bioscience division by Lonza

Sartorius acquires B. Braun Biotech

Sartorius Stedim Biotech is established by the reverse merger of Sartorius AG‘s Biotech division into Stedim

Sartorius acquires Wave Biotech

Sartorius acquires

Sartorius acquires Vivascience

Complete offering with complementary product lines to address all your bioprocessing needs

Long-term Collaboration

2013 2015

Sartorius acquires UK-based CRO, BioOutsource

50 years of culture media experience backed by 150 years of pharma industry innovations have led to long-term relationships with dual sourced raw material suppliers which guarantees supply and quality as well as excellent regulatory support for all your projects.

The Cell Culture Media division of Sartorius is pleased to present to you this catalogue which displays the wide variety of products we offer.

Sartorius acquires Cellca – a cell line development company

10

Flexibility

Verviers, Belgium

Sartorius helps its customers develop and manufacture biotech medicines reliably, quickly, and economically. Sartorius operates as a global player in dynamic markets around the world. Yet the group has retained much of its home-grown identity – strong regional ties and a personal corporate culture that has remained strongly intact since the company’s origins as a family business.

Today, more than 6,000 people work together at Sartorius worldwide. They hail from a diversity of cultures with different traditions, work styles and languages. What unites all employees at Sartorius is their strong identification with the company and its common vision, mutual goals and strategies, as well as a common set of values they all share: Sustainability, Openness and Enjoyment.

The Sartorius Bioprocess Solutions Division is a leading international provider of integrated solutions for the biopharmaceutical industry. With our extensive portfolio of products, technologies and services, we cover wide areas of the biopharmaceutical process chain, ranging from fermentation, cell cultivation, filtration and

purification to media storage and transportation. In process filtration, fermentation and fluid management, our company holds leading positions in the markets it serves across the globe. Our single-use products that have meanwhile become established in nearly all process steps are playing an increasingly important role in the manufacture of biopharmaceuticals. As a result, we help our customers produce biological medications and vaccines safely and efficiently.

k Assurance of supply – dual production sitesk Batch size scalability – liquid media: 1 L to 10,000 L k Powder media: 7 kg to 7,000 kg k Wide range of primary and secondary packaging

to meet specific needs k Off-the-shelf and fully customized solutions

and formulations k Media formulation options include animal origin

(AO) and non-animal origin (NAO), serum-free, protein-free and chemically defined with full traceability to provide solutions for any type of process

k Media formulation options for a broad range of cells such as hybridoma, CHO, insect cells, kidney cells, primary cells, stem cells and microorganisms

Features

11

In addition to off-the-shelf media and buffers, Sartorius also offers tailor-made production of Lonzas' proprietary formulations or of our customers’s own formulations. We offer a wide array of liquid and powder solutions along with the expertise to help you develop your project from clinical trials to commercialization. Offered

Dedicated support from both Sartorius and Lonza teams are assigned for each custom manufacturing project. Each project is treated with high confidentiality and matches customer requirements.Specific documentation is available to help our customers build a regulatory dossier.* up to 4000 L in small bags or bottles for AO and NAO media and up to 10000 L in big bags (> 50 L size)

Please contact your local representative for more detailed information on how Sartorius can support your project.

Fully Traceable Raw Materials for Media and Disposable System Scalability from R&D to Commercialization

Process Development

Scale-Up | Pilot Plant

CTM (Clinical Trial)Launch Facility

Commercial Manufacturing

Walkersville, MD, USA

Walkersville MD, USA Verviers, Belgium

cGMP (21CFR820) based manufacturing facility ✓ ✓

ISO 9001:2008 certified ✓ ✓

ISO 13485:2008 certified ✓ ✓

Liquid media capabilities (per batch) Up to 10,000 L Up to 10,000 L*

Powder media capabilities (per batch) NAO Medium

Up to 700,000 L Up to 100,000 L

Batch capacity 7-7290 Kg 20-1200 Kg

products and services include specially engineered media and buffers for dedicated USP | DSP | F&F unit operations. Taking benefit of the 2 manufacturing sites of Lonza in Belgium (Verviers) and USA (Walkersville), we offer a capacity of liquid production up to 10,000 L per batch and up to 7 tons of powder.

Custom Production

Cell LineDevelopment

12

A broad range of off-the-shelf media are available in liquid and powder format to provide you with maximum flexibility during early development.We offer special kits for cell line and media development as liquid (1 – 20 L) and as powder versions (up to 5 kg).

Our Range of Cell Culture Media

Cells Medium Culture Primary application

CHO (e.g., DG44, CHO-S, CHO K1, DHFR–)

UltraCHO Suspension and Adherent Proteins

ProCHO®-5 Suspension Proteins

PowerCHO™-2 Suspension Proteins

PowerCHO™-3 Suspension Proteins

ProCHO®-AT Adherent Proteins

PowerCHO™ Advance Suspension Proteins

CHOK1SV (GS platform) PowerCHO™-GS Suspension Proteins

Lonza GS V8 Media and feeds* Suspension Proteins

Hybridoma HL-1 Suspension Proteins

ProDoma-3 Suspension Proteins

UltraDoma Suspension Proteins

UltraDoma PF Suspension Proteins

NS0 ProNS0 Suspension Proteins

Insect cells (e.g., Sf9, Hi 5) Insect Xpress Suspension Proteins | Vaccines

Vero ProVero-1 Adherent Vaccines

PC-1 Adherent Vaccines

ProMDCK Suspension and Adherent Vaccines

MDCK UltraMDCK Adherent Vaccines

ProMDCK Suspension and Adherent Vaccines

HEK 293 Pro293a Adherent Vaccines

Pro293s Suspension Vaccines

PerC6* Permexcis* Suspension Vaccines

PerC6 (and retinoblastoma cell lines) ProPER1 Suspension Vaccines

Hematopoietic cells (e.g., T, NK, DC)

X-Vivo 10 Suspension Cell therapy



HL-1 Suspension Cell therapy

Mesenchymal stem cells MSCGM CD Adherent Cell therapy

Adult dermal fibroblasts FGM CD Adherent Cell therapy

Adult and neonatal primary keratinocytes

KGM CD Adherent Cell therapy

NAO: Non-animal originCD: Chemically defined* License required

Upstream Applications

Sartorius can offer specialty media dedicated to vaccines and cell therapy applications depending on the cell lines used. Sartorius offers mainly serum-free media and some of them are non-animal origin (NAO) certified to ensure maximal safety for customers whose goal is to develop cell cultivation processes dedicated to dedicated to protein manufacturing, vaccines and regenerative medicine applications…

Upstream Processing

USP

Multi Purpose Media p.14

Ultra Culture™ p.14

Insect-Xpress™ p.15

Recombinant Proteins p.17

PowerCHO™ Protein Expression Media

p.18


ProCHO™ p.22

Feeds p.23

GS-CHO p.24

CHO Media - The Optimization Tool Box

p.26

CHOptimizer: The Media Builder Box

p.28

ProNSO™ p.30

UltraDoma™ p.32


ProDoma™ p.34

Viral Vaccines p.35

ProMDCK™ p.36

UltraMDCK™ p.37

ProVero™ 1 p.38

ProPer™ 1 p.39

PERMEXCIS® p.40

Pro293™ p.42

Regenerative Medicine

p.45

X-Vivo™ p.46

L7™ hPSC Culture System p.48

FGM-CD™ Fibroblast Growth Media

p.50

KGM-CD™ p.51

MSCGM-CD™ p.52

14

Ordering Information

UltraCULTURE™

UltraCULTURE™ Medium is a serum-free medium designed for the cultivation of a wide variety of mammalian cell types. The medium consists of a DMEM:F-12 base which is supplemented with recombinant human insulin, bovine transferrin and a purified mixture of bovine serum proteins including albumin. The total protein concentration of UltraCULTURE™ Medium is approximately 3 mg/mL.

UltraCULTURE™ Medium does not contain L-glutamine. UltraCULTURE™ Medium has been used to grow a variety of cells of primary origin and established cell lines. The medium may be used to support the fusion of cells during the formation of hybridomas.

UltraCULTURE™ Medium has been used to grow cells of lymphoid origin including monocyte and macrophage cell lines. Epithelial and fibroblastic cells have been cultivated in UltraCULTURE™ Medium and endothelial cells have been cultivated under reduced serum concentration (5% fetal bovine serum using UltraCULTURE™ Medium as a base formulation).

UltraCULTURE™ Medium has also been used as a complete medium to support the generation of viral particles for use in vaccine production. UltraCULTURE™ Medium may be supplemented with cryoprotective medium to cryopreserve cells in a serum-free environment.

EB-3 Human Burkitt’s lymphoma

K-562 Human Chronic myelocytic leukemia

HNK Human Neonatal kidney (primary)

HTC29 Human Colon Cancer

TT Human Medulary thyroid tumor

MB231 Human Breast carcinoma

U138 Human Glioma

FM3A Mouse Breast cancer

NS-1 Mouse Myeloma

Cell line Source Cell type

HEL, N-10 Human Fetal lung diploid fibroblast

HeLa Human Uterine cancer

HuL-1,2 Human Liver (normal)

HuK-1 Human Kidney (normal)

HuS-1AT Human Skin

HEC Human Embryonic cancer

HL-60 Human Acute promyelocytic leukemia

Raji Human Burkitt’s lymphoma

Partial List of Cells Cultivated with UltraCULTURE™ Medium:

L Mouse Subcutaneous

P388D1 Mouse Macrophage-like

P815 Mouse Mast cell tumor

T3 Mouse Pituitary

B82 Mouse L cells –connective tissue

RPL-1 Rat Peritoneum

RSP-2 Rat Spleen

RLG-1 Rat Lung

Lym-1 Rat Lymph node

RCR-1 Rat Brain

235-1,MNQ Rat Pituitary

GC, GH3 Rat Pituitary

CA77 Rat Medulary thyroid tumor

Rat-1 Rat Fibroblast

JTC-12 Monkey Kidney

COS1, COS7 African green

monkey

SV40 transformed kidney

Features of the standard product

Storage 2ºC-8ºC


Cat. No. NA Cat. No. EU Product Description Size

BP12-725F BEBP12-725F UltraCULTURE serum-free w/o L-Gln 500 mL 500 mL

15

Multi Purpose Media

Related Products


Insect-XPRESS™ Medium was developed to support the growth of invertebrate cell lines, derived from the fall army worm, Spodoptera frugiperda mainly Sf9 and Sf21.The Insect-XPRESS™ Medium formulation is protein-free (all components are less than or equal to 10,000 mwt.) and has been designed to support the growth of insect cells under attachment-dependent or attachment-independent conditions.

Cell densities in excess of 8.3 + 106 cells/mL have been achieved with suspension cultures of Sf9 cells using Insect-XPRESS™ Medium and an excess of oxygen. Insect-XPRESS™ Medium may also be used for stationary monolayer cultures and shakeflask cultures. The Insect-XPRESS™ 1X liquid formulation contains L-glutamine and supports the production of recombinant proteins by cells infected with viral vectors such as the Baculovirus. Insect-XPRESS™ Medium may be used as a base for the cryopreservation of insect cells. We recommend that the Insect-XPRESS™ Medium liquid be mixed 50:50 with Cryoprotective Medium (Cat. No. 12-132) prior to cryopreservation.

Applicationsk Baculovirus propagationk Recombinant protein expression

Insect-XPRESS™

Product Name Page

ProFreeze™CD(2X) FreezeMedium – Chemically Defined 69

Grace‘s Insect Medium 89

Storage 2ºC-8ºC


Cat. No NA Cat. No EU Product Description Size

BE12-730P10 BE12-730P10 STD Insect-XPRESS w/L-Gln, 10 L bag 10 L

BP12-730Q BEBP12-730Q Insect-XPRESS w/L-Gln 1 L 1 L

BP12-730F BEBP12-730F Insect-XPRESS w/L-Gln, 500 mL 500 mL

Recombinant ProteinsRecombinant proteins are produced after insertion of the gene of interest into a suitable host cell genome. Cell culture media is needed in all upstream processes with animal cells as expression hosts. Choice of media depends on the cell line used, which itself is selected depending on the product of interest. In the early days of cell cultivation, the easiest way to promote cell growth was adding serum. Still today, in many lab applications, serum is a multipurpose supplement.

Serum is a natural product, which varies in composition and bears risk of infectious reagents, which is why serum-free media (SFM) are preferred. New generations of SFM contain no components of animal origin (classified as NAO). Downstream processing is simplified due to the medias' reduced protein content.

We offer a full range of products such as standard or customized cell culture media with various features, such as serum-free, protein-free, chemically defined and non-animal origin.Although the media is important, we also consider the entire process to bring you a complete and fit-for- purpose solutions through our fully complementary systems across the entire Sartorius portfolio.

Recombinant Proteins


p.18


ProCHO™ p.22

Feeds p.23

GS-CHO p.24

CHO Media – The Optimization Tool Box

p.26

CHOptimizer – MBB: The Media Builder Box

p.28

ProNSO™ p.30

UltraDoma™ p.32


ProDoma™ p.34

18

PowerCHO™ Chemically Defined, Serum-free CHO Media are the next generation in CHO media, optimized for cell growth and protein production. The PowerCHO™ Series are hydrolysate-free, serum-free and non-animal origin media for supporting high density CHO cells in suspension. For therapeutic bioprocessing applications, these protein-free formulations also facilitate both down-stream purification and regulatory compliance.


Our years of extensive experience in providing serum-free CHO media to research and industrial customers provides the basis for making great media even better. Completely chemically defined and serum-free, these media outperform with multiple cell lines.

PowerCHO™ Media deliver many unique benefits including consistent results, simplified downstream purification, and maximized yield.

Whether or not you choose serum-free media for the convenience and consistency in your research applications, or if your work leads to downstream therapeutic or biopharmaceutical applications, PowerCHO™ Media offer the best choice for initiating your studies. To learn more about PowerCHO™ Media and other Specialty Media products, contact Sartorius.



Storage 2ºC-8ºC

19




12-771P10 BE12-771P10 STD PowerCHO™-2 CD, w/o L-Gln w/o PR, 10 L 10 L

12-771P20 BE12-771P20 STD PowerCHO™-2 CD, w/o L-Gln w/o PR, 20 L 20 L

15-771ND BE15-771ND STD PowerCHO™2 Powder kit 10 L 10 L

15-771NF BE15-771NF STD PowerCHO™2 Powder kit 50 L 50 L

15-771NJ BE15-771NJ STD PowerCHO™2 Powder kit 100 L 100 L

BE15-776D BE15-776D STD PowerCHO™ GS Powder kit 10 L 10 L

N/A BE15-770F STD PowerCHO™1 Powder kit 50 L 50 L

N/A BE15-771L STD PowerCHO™2 Powder kit 500 L 500 L

N/A BE15-776F STD PowerCHO™ GS Powder kit 50 L 50 L

BP12-770Q BP12-770Q PowerCHO™-1 serum-free medium 1 L 1 L

BEBP12-776Q BEBP12-776Q PowerCHO™ - GS, 1 L bottle 1 L

N/A BEBP02-042Q PowerCHO™-2 CD, w/o glucose, 1 L 1 L

BP12-771Q BEBP12-771Q PowerCHO™-2 CD, w/o L-Gln w/o PR or HT,1 L 1 L

BP12-772Q BEBP12-772Q PowerCHO™-3 CD, w/o L-Gln w/o PR or HT,1 L 1 L

Related Products

See list of feeds p.23.

20

The new PowerCHO™ Advance™ allows superior performance when compared to leading CHO media in the market. PowerCHO™ Advance™ provides optimal conditions in shake flasks as well as bioreactors to allow flexibility to all customers working with CHO cells. This new and improved version of PowerCHO™ allows for greater filterability and easy scale up with a regulatory friendly product.

Storagek 2°C to 8°C in a dark location is recommended before and

after L-glutamine supplementationk When stored in proper conditions this media can be used for

12 months from the date of manufacture


Key featuresk Chemically defined, no raw materials of animal origin,

serum-free and manufactured to regulatory standardsk Designed for growing and feeding CHO cells in serum-free

conditionsk Designed to work together as a complete system which allows

for easy scale upk PowerCHO™ Advance™ as a basal formulation and PowerFeed™

Advance™ as a feed for fed-batch culturesk Allows for easier filtration while maintaining cell growth and

viabilityk Provides protein titers equivalent or better compared to

competitorsk Labeled “For Further Manufacturing Use”k For cell culture use only and not approved for human or

veterinary use and neither for application in humans or animals, or for use in vitro diagnostic or clinical procedures

Advance Compared to Competitors

Storage 2ºC-8ºCPowerCHO™ Advance™


0%

20%

40%

60%

80%

100%

120%

0

50

100

150

200

250

300

350

400

PC Adv-PF Adv

A1-a1 A1-a2 A2-a1 A2-a2 B1-b1 B2-b1 C1-c1 D1-d1 D1-d2

Tota

l IgG

1

Tota

l cel

l (x

106 )

PowerCHO™ Advance with Feed Compared to Competitors in Shake Flasks - DP12 CHO

Day5 - total cells Day6 - total cells Day7 - total cells Day8 - total cells

Day9 - total cells Day10 - total cells Day12 - total cells

21


Upcoming new product:For more information about its availability please contact your local MPM or email [email protected]

Cat. No. NA Cat. No. EU Product Name Size Options on Request

12-927Q 12-927Q STD PowerCHO™ Advance™ 1 L


Related Products


22


The following media systems are available:

k ProCHO™ 4 Medium – For concurrent transition of adherent CHO cells to serum-free and suspension culture; supports faster doubling times. Cat. No. 12-029Q contains phenol red and 0.1% Pluronic® F-68. Does not contain L-glutamine, hypoxanthine, or thymidine. Cat. No. 04-919Q is similar to 12-029Q except that it does not contain phenol red.

k ProCHO™ 5 Medium – For CHO cells already growing in suspension; supports increased protein production. Contains 0.1% Pluronic® F-68. Does not contain L-glutamine, phenol red, hypoxanthine, or thymidine.

k ProCHO™ AT Medium – For adherent culture of CHO cells. Contains L-glutamine. Does not contain hypoxanthine or thymidine.

ProCHO™Non-animal Origin CHO Expression Media


Features of the standard productProCHO™ Protein-free CHO Media were developed speci fically to facilitate the production and downstream processing of recombinant proteins expressed in CHO cells. These protein-free formulations support high-density cultures without the need for animal-derived components. Very low levels of recombinant insulin facilitate both downstream puri cation and regulatory compliance.

Storage 2ºC-8ºC


15-766D BE15-766D STD ProCHO™ 5 Powder kit w/o PR 10 L 10 L

15-766F BE15-766F STD ProCHO™ 5 Powder kit w/o PR 50 L 50 L

15-766L BE15-766L STD ProCHO™ 5 Powder kit w/o PR 500 L 500 L

BE12-766P10 BE12-766P10 STD ProCHO™ 5w/Pluronicw/oL-Gln, HT,10 L 10 L

BE12-766P20 BE12-766P20 STD ProCHO™ 5w/Pluronicw/o L-Gln, HT,20 L 20 L

BP04-919Q BP04-919Q ProCHO™ 4 w/o Phenol Red, 1 L 1 L

BP12-029Q BEBP12-029Q ProCHO™ 4 w/o L-Gln, HT 1 L 1 L

N/A BEBP02-041Q ProCHO™ 5 w/o glucose, 1 L 1 L

BP12-766Q BEBP12-766Q ProCHO™ 5 w/Pluronic w/o L-Gln or HT, 1 L 1 L

BEBP02-016Q BEBP02-016Q ProCHO™ AT w/L-Gln 1 L, w/o HT 1 L

Related Products


23

Product Lipids Glucose Trace Elements Amino Acids Vitamins Hydrolysates Proteins

ProCHO4 h h h h h h -

ProCHO5 h h h h h h -

PowerCHO™-1 h h h h h - -



PowerCHO™ Advance™

h h h h h - -

CHOptimizer* h h h h h - -

Which of our CHO Media better fits your requirements?


* Learn more about the CHOptimizer on page 26.

“ PowerFeed™ A and Xtreme™ feeds are formulated to maximize cell growth using Lonza CHO cell culture media ProCHO™ 4, -5 and PowerCHO™ 2.

PowerFeed™ A and Xtreme™ feeds are Chemically Defined and Protein-free feeds. Both products are formulated using all Non Animal Origin components.”

Feeds


02-044Q BE02-044Q STD PowerFeed A, 1L bottle 1 L

15-044D BE15-044D STD PowerFeed A Powder kit 10 L 10 L

15-044F BE15-044F STD PowerFeed A Powder kit 50 L 50 L

15-044J BE15-044J STD PowerFeed A Powder kit 100 L 100 L

15-044Q BE15-044Q STD PowerFeed A Powder kit 200 L 200 L

BE02-056Q BE02-056Q STD CHO Xtreme Feed™ CD - 1 L bottle 1 L

24


Lonza’s powerful proprietary GS System™ uses a robust viral promoter and selection via glutamine metabolism to provide extremely rapid development of high-yielding, stable, cGMP-compatible mammalian cell lines.

The GS System™ is successfully used by over 85 global pharmaceutical and biotechnology companies and is familiar to all worldwide regulatory authorities.

The international use of the GS System™ by both commercial organizations and academic institutions has led to a large body of published information. New users can draw upon this material to expand their knowledge and skill base and improve their use of the GS System™.

GS-CHO™Version 8 (v8) Commercial Platform ProcessLatest version of the cell culture media and feed platform for the GS Gene Expression System™. This new offering includes Lonza’s proprietary chemically defined, animal component-free (CDACF) Version 8 (v8) Media and Feeds Platform which was designed in conjunction with the GS-CHO cell line.

The new v8 Platform incorporates GS-CHO specific CDACF cell culture medium, feed base powders and supplements and is globally manufactured and supplied by our custom media production facilities. The CDACF v8 Platform has been shown to reach yields up to 9.6 g/L and has also been used successfully with DHFR-CHO cell lines. This media is free from both royalty and license fees and is available to all holders of the GS Gene Expression System™.

The Version 8 AdvantageChemically defined and animal component-free (CDACF)Developed by Lonza specifically for GS-CHO cell linesNo royalties or license fees addedIncreased antibody yieldImproved cellular metabolism


25



BE02-043E BE02-043E STD FeCitrate1000x PowerCHO™-GS kit,100 mL 100 mL

BE15-776D BE15-776D STD PowerCHO™ GS Powder kit 10 L 10 L

N/A BE15-776F STD PowerCHO™ GS Powder kit 50 L 50 L

BEBP12-776Q BEBP12-776Q PowerCHO™ - GS, 1 L bottle 1 L

BESP904 BESP904 Lonza medium base 1 20,18 g

BESP905 BESP905 Feed Base K 10,23 g

BESP906 BESP906 Feed Base L 28,7g

BESP907 BESP907 Feed Base M 2,36 g

BESP908 BESP908 Feed Base N 0,18 g

BESP799 BESP799 Feed base F powder 1,75 g

BESP862 BESP862 Feed base E powder 1,72 g

BESP808 BESP808 CHO Vitamin Mix 0,18 g

BESP794 BESP794 Feed Base A 43,06 g

BESP795 BESP795 Feed Base B 112,2 g

BESP734 BESP734 NMB1 22,04 g

BESP735 BESP735 NMB2 18,34 g

BESP1023 BESP1023 Feed Base R 114,52 g

BESP876 BESP876 Feed Base 2 6,47g

BESP887 BESP887 MEM Vitamin Powder 9,31 g

BESP899 BESP899 Feed Base G 42,63 g

BESP900 BESP900 Feed Base H 69,04 g

BESP901 BESP901 Feed Base J 133,87 g

BESP1021 BESP1021 LMdB2 19,97 g

BESP1022 BESP1022 Feed Base P 72,17 g

BESP797 BESP797 Feed Base D 51,88 g

BESP531E BESP531E SPE 100 mL

BESP531F BESP531F SPE 500 mL

BESP531Q BESP531Q SPE 1000 mL

BESP909E BESP909E LTEM ONE 100 mL

BESP909Q BESP909Q LTEM ONE 1000 mL

BESP910E BESP910E LTEM TWO 100 mL

BESP910Q BESP910Q LTEM TWO 1000 mL

BESP969E50 BESP969E50 SP6 50 mL

BESP969F BESP969F SP6 500 mL






BESP807E BESP807E CHO Trace Element Stock Solution 100 mL

BESP807Q BESP807Q CHO Trace Element Stock Solution 500 mL


CHO Media – The Optimization Tool Box

26

Benchmarking KitStandard Media Testing

Standard solutionsFast and EasyNo Optimization

CHOptimizer

The Media Builder BoxFast Optimization processFinal Formulation under controlFit-for-purposeProcess Optimization

Do It Yourself

State of the art multi-bioreactor (ambr) linked to DoE software under AS support

Fully outsourced process bringing you a turnkey solution


The biopharmaceutical industry uses mainly animal cell cultures for the production of proteins targeting human and animal diseases, the most commonly used cell line being CHO. These processes are highly challenging due to multiple factors, such as:

– Regulatory authorities, such as the FDA and the EMA, that supervise production processes and products to ensure the safety of commercial products

– Variability of cells and proteins that are produced– Variability of culture conditions and parametersIt is impossible to have one fit-for-all media, as cell lines all have their specificities and all need different feeding routines to outperform.

The best approach for you depends on several parameters such as your timeline, your target market, your cells, your product lifecycle, etc. We strongly believe in a systemic approach, giving you the flexibility to navigate between standard media to fully optimized custom media.

27

Media OptimizationService Project

Start from “scratch”Full serviceNeed to ship your cellsFully CustomizedOutsource and save


28

CHOptimizerThe Media Builder BoxBiopharmaceutical industry uses mainly animal cell cultures for the production of proteins targeting human and animal diseases. New development processes are complex and highly challenging due to multiple factors as:

k Variability of the cell lines, vectors and proteins to produce and other requirements such as quality attributes

k Variability of the culture conditions and parameters k Quantities needed are variable, application dependent, with focus on

quality, time lines and cost-effectivenessk Regulatory authorities, e. g. FDA, EMA, supervise production processes

and products to ensure compliance and safety of the commercial product

A Service k Application Specialist – Full customer support

4 basal media k DoE run of 21 blends + 3 controls/center points

Full Protocols k Optimized process parameters

A 3 phases approach k Media Composition linked to Supply Agreement

Results in a unique custom medium and feed with a custom feed strategy.

CHOptimizer includes:– ~10 days of Application Specialist work– Media supplies including optimized media

and feeds– DoE software analytics– Media Spent Analysis (96 tests)– ambr15 and consumables

Featuresh Coupled DOE softwareh Reduce time to market with fully

optimized mediah Platform approach with control on process

and costs.h State-of-the-art multiple parallel single

use automated stirred-tank bioreactorsystem (ambr)

h No need of shipping cell line

Complementary products– Benchmarking Kit: Standard product

testing– Application Specialist Support– TAP ambr system– Media Optimization Tool Box– Cellca offerings

CHOptimizer

First run 24 blends from

4 base solutions

First run 16-39 blends from

4 base solutions

Use of ambr Second run in house

YES

NO

PHASE I


28

29

CHOptimizerThe Media Builder Box

How does it fit strategically?

Second run 3-4 blends from DoE

simulation 2-3 replicates

Process Optimization

Second run 3-4 blends from DoE

simulation 2-3 replicates

Third run in house

Third run Final solutions with

feedsProcess/Feed

StrategyOptimization

Third run Final solutions with

feeds

Media Optimized

Custom Optimized Media

PHASE II PHASE III


Value Added Media Optimization Service Media Benchmarking Kit CHOptimizer

Flexibility - ++ ++

Platform Approach -- -- +++

Plug and Play -- + +++

Time to market --- ++++ ++

Optimized Media +++ -- +++

DoE Approach (++) -- +++ Ambr + MODDE

30

ProNS0™ Protein Expression MediaThe NS0 cell line is widely used for recombinant mammalian protein expression. Some of the reasons to select this mouse myeloma platform are:– Forms stably producing hybrid cells with high levels of protein

production– Lacks ability to secrete endogenous antibody or antibody

fragments– Resists aggregation clumping in suspension culture– Lacks adverse protease activity on recombinant protein product

(in many cases)

BioWhittaker® ProNS0™ 1 Chemically Defined, Protein-free Media, together with ProNS0™ Lipid CD Supplement, are designed to meet a growing demand for optimized NS0 formulations.



BP12-773Q BP12-773Q ProNS0™ 1 CD 1 L

BP12-775J BP12-775J ProNS0™ Lipid CD 5 mL

ProNS0™ Mediak Two optimized formulas are available to cover the

broadest range of NS0 nutritional needsk Further maximize protein production by titration with

ProNS0™ Lipid CD Supplementk Protein-free and chemically defined formulationsk Product purification is simplifiedk Lot-to-lot consistency ensures dependable performancek Non-animal origin components reduce regulatory

burdens

This trio of NS0 products provides the answer to maximal protein productivity.

ProNS0™ Chemically Defined, Protein-free MediaTwo distinct formulas are offered to cover a broad range of nutritional needs. Titering the separate lipid supplement permits further optimization that can have a significant positive impact on protein production. Suggested starting concentration for optional addition of ProNS0™ Lipid CD Supplement is 5 mL per liter.

Basal Media 2ºC-8ºCLipid Supplement -10ºC--20ºC



31

Day 6 mg/mL

0

0.05

ProNS00 ™ 1 CD

Competitor 1 Competitor 2 Competitor 3

0.1

0.15

0.2

Figure 1. ProNS0™ Media outperform competitive media for recombinant IgG production in NS0 cells. Duplicate 125 mL shaker flasks were seeded at a density of 200,000 NS0 cells per mL in a 30 mL volume. Shake rate was 100 rpm. Cells were cultured in their respective test media for one passage prior to test initiation.

IgG

(mg/

mL)

ProNS0™ Media

Comparative protein production data are presented in Figure 1. All media were prepared according to manufacturer instructions. ProNS0™ Media were supplemented with 5 mL per liter of ProNS0™ Lipid CD. Competitive media either contained lipid or were supplemented with manufacturer’s companion lipid according to manufacturer recommendations. The batch cultures were not fed or further supplemented.

Samples were taken daily to measure viable cells and IgG concentration. Cell counts and viability were measured using a Vi-Cell™ Viable Cell Analyzer (Beckman Coulter). IgG concentrations were measured by HPLC.

The data show that both ProNS0™ 1 and 2 Chemically Defined, Protein-free Media yield superior protein production compared to competitive media.


32

UltraDOMA™

UltraDOMA™ Serum-free Hybridoma Medium is a formulation designed for the cultivation of murine, human, and chimeric hybridomas in batch culture and in hollow fiber bioreactors.UltraDOMA™ Medium is supplemented with recombinant human insulin, bovine transferrin and bovine albumin. The total protein concentration is 30 μg/ml. UltraDOMA™ Medium does not contain L-glutamine.Cells that are adapted for growth in UltraDOMA™ Medium can be maintained in the medium indefinitely and can be cryopreserved in UltraDOMA™ Medium supplemented with Cryoprotective Freezing Medium.

Applicationsk Hybridoma cell growthk Monoclonal antibody production

Partial List of Cell Types Cultivated with UltraDOMA™ Medium

Cell Type

Murine hybridomas

NS-1 derived myelomas

SP-2 derived myelomas

Human hybridomas (with 0.5% FBS)

Performance and Quality TestsUltraDOMA™ Medium contains no human-derived proteins


Storage 2ºC-8ºC




N/A BEBP12-723F UltraDOMA™ serum-free w/o L-Gln 500 mL 500 mL

BP12-723B BP12-723B UltraDOMA™ SF hybridoma medium, 500 mL 500 mL

33

UltraDOMA-PF™Partial List of Cell Types Cultivated with UltraDOMA-PF™ Medium

UltraDOMA-PF™ Protein-free Hybridoma Media are formulations designed for use with hybridoma cell lines of murine, human, and chimeric origin. UltraDOMA-PF™ Media are completely defined media and do not contain peptides or tissue extracts. The use of UltraDOMA-PF™ Media significantly simplifies downstream processing since all proteins present in a given cell culture supernatant are produced by the cells.UltraDOMA-PF™ Media are designed for lab scale or industrial scale use. It is available as a 1X liquid or as a powder.L-glutamine and HEPES buffer are included in the formulation.

Applicationsk Hybridoma and myeloma growthk Monoclonal antibody production

Cell Type

Murine hybridomas

NS-1 derived myelomas

SP-2 derived myelomas

Human hybridomas (with 0.5% FBS)

Rat hybridomas

Some transfected Chinese Hamster Ovary (CHO) cell lines

Human lymphoid origin cells

Murine lymphoid origin cells


Storage 2ºC-8ºC




BP12-727F BP12-727F UltraDOMA-PF™ Hybridoma Medium, 500 mL 500 mL

34

ProDoma™

ProDoma™ Serum-free Hybridoma Media is designed for cultivation of murine, human, and chimeric hybridomas.

ProDoma™ Media are protein-free with a low amount of human recombinant insulin. All ProDoma™ Media include HEPES as well as sodium bicarbonate in the formulation.

Applicationsk Hybridoma cell growthk Monoclonal antibody production


Storage 2ºC-8ºC



BEBP02-029Q BEBP02-029Q ProDoma™ 1 serum-free medium, 1 L 1 L

BEBP02-032Q BEBP02-032Q ProDoma™ 3 woL-gln,PR w 0.1% Pluro F68 1 L 1 L


35

Viral vaccines are produced by viral infection of susceptible animal cells or by recombinant expression in suitable host cells. A variety of adherent cell lines are used for production of viral vaccines, mostly cells derived from kidneys, such as HEK293, Vero, MDCK, and BHK21, although others cell types can also be used (EB66, PerC6). Specialty media offered in this category have been designed to allow maximum cell growth and infection rate.

Viral Vaccines

Viral Vaccines

ProMDCK™ p.36

UltraMDCK™ p.37

ProVero™ 1 p.38

ProPer™ 1 p.39

PERMEXCIS® p.40

Pro239™ p.42

36


Related Products

Cat. No. NA Cat. No. EU Product Name Size

12-749Q 12-749Q UltraMDCK™ 1 L

17-605 17-605 L-glutamine, 200 mM 100 mL

ProMDCK™ are serum-free media dedicated to the growth and infection of Madin-Darby Canine Kidney (MDCK) cells in all conditions, either for research of for further manufacturing purposes. ProMDCK™ has been optimized for both expansion and virus infection of MDCK cells in planar culture conditions (2D version) and in stirred bioreactors, bound onto microcarriers (3D version).

Applicationsk 2D version: MDCK cells growth in planar culture for research

(cell biology studies such as the mechanisms of passive salt, protein, lipid and drug transports, in vitro growth regulation and cytotoxicity studies), bioreactor seeding or QC tests

k 3D version: MDCK cells infection with virus (Influenza strains, poliovirus…) for vaccines manufacturing

ProMDCK™ media – 2D and 3D versions

Featuresk Easy to scale-up: Provides a suitable environment for MDCK

cells for all upstream steps (seeding, growth, infection) and manufacturing scales (T-flask to bioreactor) without any transition phase or adaptation needs.

k Efficiency: Increases doubling time and virus productivityk Regulatory-friendly: Serum-free media manufactured

following cGMP (21CFR820 guidelines) for maximum safety

Storage 2ºC-8ºC



NEW!


12-924Q N/A ProMDCK™ (2D) 1 L

12-925Q N/A ProMDCK™ (3D) 1 L

37

UltraMDCK™

UltraMDCK™ Medium is a serum-free and chemically defined medium designed to support the growth of MDCK cells at low and high plating densities. UltraMDCK™ is an optimized basal medium supplemented with only two proteins – recombinant human insulin and bovine transferrin, manufactured following cGMP guidelines and yielding a very low protein content formulation.

The MDCK (Madin Carby Canine Cells) cell line was originated by S.H. Madin and N.B. Darby in 1958 from a kidney of an apparently normal, adult, female cocker spaniel. The cells are heteroploid with an epithelial like morphology.

MDCK cells grown in UltraMDCK™ medium are smaller and more densely packed than cells grown in the presence of serum. Moreover, cultures can stay confluent for at least two weeks without medium change. Cells will continue to grow from the monolayer forming spherical structures called “floaters”. “Floaters” can be harvested, pelleted by centrifugation and plated into fresh medium. They will re-attach and grow into a new monolayer.

A monolayer of MDCK cells is difficult to trypsinize especially when grown in a serum-supplemented medium. However, when grown in UltraMDCK™ medium, trypsinization becomes less difficult. UltraMDCK™ medium is offered in 1 liter plastic bottles as a “complete” medium. UltraMDCK™ medium has a shelf-life of one year.

Featuresk Efficiency

Superior cell growth characteristics beyond confluence and high viral productivity

k Regulatory-friendly A defined serum-free medium environment without the inconsistencies and concerns of serum and manufactured following cGMP with selected raw materials

k Simplified downstream process Easy purification procedures in manufacturing scale as it is chemically defined and contains only one protein and one peptide

ApplicationsMDCK cells are used in the isolation of influenza A and influenza B viruses. They have been found to be susceptible to vesicular stomatitis (Indiana strain) virus, vaccina, Coxsackie B-5, reovirus types 2, 3 and adenovirus types 4 and 51. MDCK cells have been used in functional studies such as the mechanisms of passive salt transport2, protein3, lipid4 and drug5 transport or in vitro growth regulation studies6 and cytotoxicity tests7. MDCK cells are currently used for marketed polio and flu vaccines manufacturing.


Storage 2ºC-8ºC


Viral Vaccines


BP12-749Q BEBP12-749Q UltraMDCK™ serum-free 1 L 1 L

38

ProVero™ 1 Serum-free Media

ProVero™ 1 Serum-free Medium is a protein-free medium designed to support the growth of Vero and MDCK kidney cells.ProVero™ 1 Medium includes HEPES and sodium bicarbonate buffer. The absence of proteins and the very low levels of human recombinant insulin facilitate both downstream processing and regulatory compliance.Some Vero cell strains require additional supplementation with 5.0 μg/L rhEGF for optimal cell growth.

Applicationsk Virus productionk Recombinant protein production


Storage 2ºC-8ºC




12-749Q UltraMDCK™ 1 L

12-769E ProFreeze™ NAO 2X 100 mL

Related Products


BEBP02-030Q BEBP02-030Q ProVero™ 1 NAO Medium for Vero Cells, 1 L 1 L

39

Related Products


L-Glutamine 100 mL

ProFreeze™ CD (2X) Freeze Medium – Chemically 100 mL


ProPer™ 1 Serum-free Medium – Chemically Defined, is an animal-origin component-free, chemically defined, serum-free medium for growth of human embryonic retinoblast cells (PER.C6® and related cell lines) in suspension. The medium contains a low amount of human recombinant insulin. HEPES and sodium bicarbonate are also present in the formulation.

Applicationsk Recombinant protein and virus productionk Growth of human embryonic retinoblast cells (PER.C6® and

related cell lines) in suspension

ProPer™ 1 Serum-free Media

PER.C6® Cells growing in ProPer™ 1 Medium.

Storage 2ºC-8ºC

Viral Vaccines



BEBP02-028Q BEBP02-028Q ProPer™ 1 wo L-gln, PR w 0.1% Pluro F68 1 L 1 L

40

PER.C6® Technology is a Crucell proprietary human cell-based platform designed for large-scale production of recombinant proteins, including antibodies, vaccines and gene therapy products, under serum-free culture conditions. Cell lines have been immortalized with specific adenovector genes, leading to high viable cell densities and high PCDs (picogram/cell/day), resulting in high yields. PERMEXCIS® Virus Production Medium has been optimized for use with the PER.C6® Cell Line. PERMEXCIS® Medium is chemically defined, serum-free, low protein (<200 ng/mL), with Pluronic® F-68, and without L-glutamine or phenol red.


Related Products

PERMEXCIS® Medium for Per.C6® cells

Featuresk Reduces regulatory hurdles with a non-animal origin statusk Maintains high viability (> 90%) at high cell densities for

superior performancek Achieves a maximum culture performance through a balanced

formulationk Saves validation time with no weaning required during growth

transition

Applicationsk Virus productionk Growth of human embryonic retinoblast cells (PER.C6® and

related cell lines) in suspension


02-028Q 02-028Q ProPER-1 100 mL

17-605E 17-605E L-Glutamine 200 mM solution 100 mL

17-605F 17-605F L-Glutamine 200 mM solution 500 mL

17-605E/U1 17-605E/U1 UltraGlutamine™ | 200 mM 100 mL

17-605F/U1 17-605F/U1 UltraGlutamine™ | 200 mM 500 mL

12-769E 12-769E ProFreeze™ NAO 2+ 100 mL

Storage 2ºC-8ºC


PERMEXCIS® medium use is restricted only to PER.C6® Cell License Holders. Shipping will not be possible without license justification (please add license number on order or inform Sartorius customer services).

Per. C6® is a trademark of Crucell.



BE02-039Q BE02-039Q GLS PERMEXCIS® virus production med, 1 L 1 L

41

Permexcis® Medium Provides Superior Growth and Cell Density with Lowest Population Doubling Times

Figure 1 PER.C6® Cells were cultured in 10 L roller bottles in several types of serum-free media. Cultures grown in Permexcis® Medium displayed a higher cell growth and the lowest population doubling time as compared to the competition.

0

1

1 2 3 4 5 6 7 8 9

2

3

4

5

6

7

Media Type Average PDT (h)Permexcis® Medium 23

Competitor A 27

Competitor B 26

Competitor C 28

Competitor D 30

Viab

le C

ell D

ensi

ty (1

06 vc/

mL)

Time (days)

Permexcis® Medium

Competitor A

Competitor B

Competitor C

Competitor D

Total cells Viability

PER.C6® Cells in Permexcis® Medium: 2 L iCeP PER.C6® Cells in Permexcis® Medium: 10 L iCeP

0

25 25

2.5 5.0 7.5 10.0 12.5 15.0

50 50

75 75

100 100

Figures 2 and 3 PER.C6® Cells grown in an intensified cell production process with Permexcis® Medium achieved a cell density above 100 ×106 (Vc/mL). PER.C6® Cells were grown in a 2 L bioreactor via perfusion method for 13 days and a 10 L bioreactor for 15 days respectively.

Cell

Den

sity

(×10

6 cel

ls/m

L)

Viability (%)

Time (days)

0

50 25

2.5 5.0 7.5 10.0 12.5 15.0

100 50

150 75

200 100

Cell

Den

sity

(×10

6 cel

ls/m

L)

Viability (%)

Time (days)

Viable Cell Density

Viral Vaccines

42

Pro293™ Serum-free MediaPro293™ Serum-free Media – Chemically Defined were optimized to support high-density growth and recombinant protein production in 293 neo-natal kidney cells (HEK293). They are chemically defined to ease regulatory compliance and downstream protein purification. They contain very low levels of recombinant human insulin, and are free of animal origin components.


Applicationsk Pro293™ s Medium – for 293 cells growing in suspension culturek Pro293™ a Medium – for 293 cells growing in adherent culturek Recombinant protein production in 293 cells

Storage 2ºC-8ºC




BP12-764Q BEBP12-764Q Pro293™a-CDM, for adherent cells, 1 L 1 L

N/A BEBP02-025Q Pro293™s-CDM, 1 L bottle 1 L

BP12-765Q BP12-765Q Pro293™s-CDM, for suspension cells, 1 L 1 L

43

Pro293™s easy adaptation to suspension culture

Pro293™s-CDM Commercial supplier A

12

10

8

6

4

2

0

Viab

le C

ells

/mL

(×10

5 )

0 1 2 3 4 5 6 7 8Days

Pro293™a supports adherent high-density cell growth

Pro293™a-CDM Commercial supplier B

60,000

50,000

40,000

30,000

20,000

10,000

0

Viab

le C

ells

(RFU

)

0 2 3 4 5Days

Viral Vaccines

In cell therapy treatments, also called regenerative medicine, cells are treated as the medical product, replacing missing or dysfunctional patient´s cells. Cells are taken from the donor, cultivated, expanded and eventually manipulated ex vivo before administrated to the patient. The provided cells can either be autologous (where the recipient is also the donor) or allogeneic (where the donor and recipient are distinct individuals). The cells used in cell therapy can be primary cells or from cell lines. The most important cells are hematopoietic cells (T or B lymphocytes, Dendritic or Natural killer cells), tissue cells (skin, etc.), and stem cells isolated from bone marrow, fat tissue or in very rare cases embryos.



X-Vivo™ p.46

L7™ hPSC Culture System

p.48

FGM-CD™ p.50

KGM-CD™ p.51

MSCGM-CD™ p.52

46

USP

Applicationsk Proliferation of PBL, TIL, LAKk Cultivation of human monocytes, macrophages, NK and

dendritic cellsk Cultivation of stem cellsk Cryopreservation and transplantation of human tissues

and organs Cell and Gene therapies

Featuresk Provides nutritionally complete and balanced environments

for a variety of primary and immortalized hematopoietic cells (PBL, LAK, TIL…)

k More efficient than classical media with serumk Serum free and defined media – regulatory friendly – for cell

or gene therapy applications

Proven efficiency on:k Human and murine LAK cellsk Peripheral blood monocytesk Human and murine TILk Human T cellsk Human and murine macrophagesk Human and murine hemotopoietic stem cells

Other human tissues

Technical Specifications:– Does not contain serum (serum free), exogenous growth

factors, artificial stimulators or undefined components– Contains human albumin but at a high quality level

(for injection) and licensed as excipient– Contains human transferrin but available also in a recombinant

form and from non-animal origin– Manufactured under current GMPs and listed with the FDA

in a product Master File

X-Vivo™ Media

The X-Vivo™ media formulations have been designed to support the generation of hematopoietic cells including like peripheral blood lymphocytes (PBL), lymphokine activated killer (LAK) cells and tumor infiltrating lymphocytes (TIL) from healthy donors or patients. These media can also support the ex vivo growth of human monocytes, macrophages, granulocytes, natural killer (NK) cells or other hematopoietic cells. They are a suitable serum free environment for primary cells but also for stem cells, cancer cells or different but related hematopoietic cell lines.

The three different formulations of X-Vivo™ (X-Vivo™ 10, 15 and 20) are similar and can be suitable for the different applications. We recommend testing samples of the different formulations for each application. These media have been referenced in a lot of scientific publications and some comparative studies have proven that X-Vivo™ is a high efficiency serum free medium suitable for numerous different cell types.

Defined media specifically designed by Lonza for high efficiency hematopoietic cell cultivation

Storage 2ºC-8ºC



47


X-VIVO™ product range (with native human transferrin)

Products with recombinant transferrin

Cat. No. NA Cat. No. EU Product Name Product Description Size

BE02-055Q BE02-055Q X-Vivo™ 10 without gentamicin nor red phenol (04-743) with recombinant

transferrin

1 L Bottle

BE02-053Q BE02-053Q X-Vivo™ 15 with gentamicin and phenol red (04-418) with recombinant

transferrin

1 L Bottle

BE02-054Q BE02-054Q X-Vivo™ 15 without gentamicin nor phenol red (04-744) with recombinant

transferrin

1 L Bottle



08-879H 08-879H STD X-Vivo 15 w/o gentamicin and pr, 5 L 5 L

BEBP04-743Q BEBP04-743Q X-Vivo™ 10 w/o Gentamycin w/o PR 1 L 1 L

BP04-380Q BEBP04-380Q X-Vivo™ 10 w/ Gentamycin and PR, 1 L 1 L


N/A BEBP04-418F X-Vivo™ 15 w/ Gentamycin and PR, 500 mL 500 mL

BP04-744Q BEBP04-744Q X-Vivo™ 15 w/o Gent or Phenol Red, 1 L 1 L


BEBP02-055Q BEBP02-055Q X-Vivo™ 10 (04-743) contains rec transf -1 L 1 L

BEBP02-053Q BEBP02-053Q X-Vivo™ 15 (04-418) contains rec transf, 1 L 1 L

BEBP02-054Q BEBP02-054Q X-Vivo™ 15 (04-744) contains rec transf, 1 L 1 L

48

L7™ hPSC Culture System

L7™ system is a culture platform (medium, matrix, subculture reagent, and cryopreservation medium) that supports every-other-day feeding of human Pluripotent Stem Cells (PSCs) in a defined system. The L7™ System is a xeno-free and fully defined system for the maintenance and expansion of human ESCs and iPSCs. The L7™ hPSC Culture System is a robust culture system that supports every other day feeding of pluripotent stem cells. As a complete solution, L7™ includes different products that are very safe and efficient if used together:

– L7™ hPSC Matrix– L7™ hPSC Medium BulletKit™ – L7™ hPSC Passaging Solution– L7™ hPSC Cryosolution


Featuresk Robust performance and compatibility with most linesk Reduced workload and increased flexibility by supporting

every-other-day feedingk Efficient differentiation into all three germ layersk Easy transition to clinical applicationsk Complete system for the maintenance of human ESCs

and iPSCs


Use all of the L7™ PSC Culture System components for proven performance with PSC growth, expansion, and maintenance.

L7™ hPSC Basal Medium and Medium SupplementThis medium was specifically designed and formulated with a basal medium and a supplement of growth factors to support long-term growth and maintenance of undifferentiated PSCs.

L7™ hPSC MatrixLonza’s recominant hPSC Matrix is xeno-free and defined. When used in conjunction with L7™ hPSC Basal Medium, it is proven to support efficient attachment, maintain pluripotency and stable karyotype for over 40+ passages in many hPSC lines.

L7 hPSC Passaging SolutionLonza’s L7™ hPSC Passaging Solution is a chemically-defined, non-enzymatic cell detachment formulation that gently dislodgesmulticellular colony fragments without the need for mechanical manipulation. This user friendly method simplifies routine maintenance of your valuable hESC and iPSC lines.

L7™ hPSC Cryopreservation SolutionLonza’s Cryopreservation Solution supports efficient recovery of cryopreserved hPSCs under xeno-free and defined conditions.

L7™ hPSC Medium BulletKit™ L7™ hPSC Matrix L7™ hPSC Passaging Solution L7™ hPSC Cryosolution

Colony Appearance

Colony Selection and Expansion

Banking Characterization



04-1193E N/A L7™ hPSC Passaging Solution 100 mL

00215550 N/A L7™ hPSC Cryosolution 50 mL

00215549 N/A L7™ hPSC Matrix 1 mg

00215506 N/A L7™ hPSC Media BulletKit™ 500 mL Basal medium + 5 mL Supplement

04-1191F N/A L7™ hPSC Basal Medium 500 mL

04-1192J N/A L7™ hPSC Supplement 5 mL

49


Reviving and Priming Reprogramming

Colony Appearance

Colony Selection and Epansion

Banking and Characterization

1 Workflow

7 Components

1 Provider

L7™

1 Clonetics™ and Poietics™ Primary Cells

2 L7™ Reprogramming Bundles

3 Nucleofector™ Technology

4 L7™ hPSC Medium BulletKit™

5 L7™ hPSC Matrix

6 L7™ hPSC Passaging Solution

7 L7™ hPSC Cryosolution

50

FGM-CD™ Fibroblast Growth MediaFeaturesk Serum-free and chemically defined medium for expansionk Maintain the fibroblasts characteristicsk Promote collagen production


Collagen Production by NHDF-Ad grown in FGM-CD™

140

120

100

80

60

40

20

0Donor I

Colla

gen

(μg)

FGM-CO™ DMEM+10%FBS

Donor II Donor III Donor IV

FGM-CO™

Fibroblast

CD90™

Collagen I

DMEM+10%FBS

Basal medium: 2°C to8°C

Supplements: -10°C to -20°C




199019 199019 FBM-CD™

199020 199020 FGM-CD™ SinglQuots kit Kit

00199041 00199041 FBM-CD™ Bullet Kit Kit

51

TheraPEAK™ KGM-CD™ Keratinocyte Growth Media

Featuresk Contains only non-animal orgin components

k KGM-CD™ medium supports both neonatal and adult keratinocytes

k Supports formation of an excellent barrier resulting in high TEER values

k Coating plates with fibronectin or other non-animal derived matrix

material is necessary for successful keratinocyte isolations with KGM-CD™

medium


Basal medium: 2°C to8°C





CC4455 CC4455 BulletKit KGM-CD™ Kit

CC-4456 CC-4456 BulletKit KGM-CD™ EA CC3255+CC4455 Kit

52

TheraPEAK™ MSCGM-CD™ Mesenchymal Stem Cell Growth Media

TheraPEAK™ MSCGM-CD™ Mesenchymal Stem Cell Growth

Medium – Chemically Defined (with L-glutamine, without

phenol red and antibiotics).


Featuresk Supports human mesenchymal stem cell growth

(multi-potent adult stem cells)

k Differentiation into osteoblasts, chondrocytes and adipocytes

k Chemically defined and serum-free

k For further manufacturing or laboratory use

Poietics™ hMSC grown in

MSCGM-CD™ Medium

Poietics™ hMSC grown in DMEM

with 10% FBS

Basal medium: 2°C to 8°C,





190632 190632 MSCGM-CD™ bulletkit Kit

192125 192125 MSCGM-CD™ SingleQuots Kit Kit

190620 190620 MSCBM-CD™ 500 mL 500 mL

Sartorius Lonza offers a full portfolio of standard or custom buffer products to optimize your process needs in all key DSP steps and applications including:– Cell harvest– Concentration | Diafiltration– Capture– Polishing – Virus clearance | Inactivation– Bulk drug substance preparation– Final fill

As well as:– Organ preservation and transport – Tissue conservation

While the emphasis in upstream cell culture processing is growth and titer optimization, the downstream focus is purification, concentration, and preservation. These process demands can result in the need for very large buffer volumes and Sartorius Lonza is well equipped to work with you on scalability and minimizing the burden associated with transport logistics. Custom buffers will be prepared to your exact specifications of composition, molarity, pH, and quality. Global manufacturing facilities follow cGMP (21CFR820) and ISO (9001:2008) regulatory protocols to ensure a consistent, reliable, and uninterrupted supply of your powdered and liquid buffer in your preferred packaging. Whether you need a standard off-the-shelf buffer or customized solution, Sartorius Lonza has an experienced team to help guide your selection and development.

Downstream Steps

Downstream Steps

The Downstream Buffer Offering

p.54

Buffer Packaging and Options

p.56

DSP Buffers p.10

PBS p.58

DPBS p.59

Hanks p.60

Earle’s p.60

Hepes p.61

Other Buffers p.61

WFI p.62

54

Downstream Steps

Sartorius offers a wide range of process buffers for purification of monoclonal antibodies, recombinant proteins, vaccine and antibody drug conjugates.

The Downstream Buffer Offering

Buffer needs and volumes can become very large as processes grow and Sartorius Lonza is well equipped to work with you on minimizing the burden associated with transport logistics.Sartorius offers an extensive array of downstream processing buffers for protein, cellular, and viral-based therapeutics. Appropriate for the techniques you perform and for each steps of your techniques (see above).

Any buffers can be prepared to your exact specifications of composition, molarity, pH… This includes:

Harvest Concentration | Diafiltration Capture Polishing Virus Clearance | InactivationBulk Drug Substance Preparation

Fill and Finish

Centrifugation Crossflow Filtration Affinity Chromatography CHR CEX VR Filtration Formulation Final FormulationWFI Equilibration Buffer Equilibration Buffer WFI Formulation Buffer Formulation Buffer

Dynamic Body Feed Flushing Buffer Wash Buffer Wash Buffer Post-Flush Buffer WFI

Washing Buffer Elution Buffer Elution Buffer Sterile FiltrationCrossflow Filtration Regeneration Buffer Regeneration Buffer UV-C Innactivation WFI

WFI UF | DF Sanitization Buffer Sanitization Buffer Flush Buffer Post-Flush Buffer

Flushing Buffer WFI Storage Buffer Storage Buffer

Washing Buffer Equilibration Buffer Dilution Buffer Dilution Buffer Solvent | Detergent Innactivation Controlled Freeze and ThawDiafiltration Buffer Solvent Buffer Freezing Buffer

Depth Filtration Sanitization Buffer Low-pH Virus Inactivation Membrane Adsoption AEX Flush Buffer WFI

WFI Acid Sanitization Buffer

Post-Flush Buffer Base Flushing Buffer UF | DFDilution Buffer Equilibration Buffer WFI

Post-Flush Buffer Diafiltration Buffer

Strip Buffer Sanitization Buffer

mAbs Generic Downstream Process Buffer Usage

k Water for injection (WFI) k Strong Acids and Bases k Balanced Salt Solutions (BSS) – Acetate buffers – Citrate buffers – Potassium buffersk Tris Formulations k Phosphate Buffered Saline (PBS)

and DPBSk Cell Harvesting buffers k Glycine

k Freezing mediak Dedicated formulation bufferk Physiological solutionsk …

As well as other buffers:k Hepesk Earlesk Hanksk D Glucose …

55

Buffers

Harvest Concentration | Diafiltration Capture Polishing Virus Clearance | InactivationBulk Drug Substance Preparation

Fill and Finish

Centrifugation Crossflow Filtration Affinity Chromatography CHR CEX VR Filtration Formulation Final FormulationWFI Equilibration Buffer Equilibration Buffer WFI Formulation Buffer Formulation Buffer

Dynamic Body Feed Flushing Buffer Wash Buffer Wash Buffer Post-Flush Buffer WFI

Washing Buffer Elution Buffer Elution Buffer Sterile FiltrationCrossflow Filtration Regeneration Buffer Regeneration Buffer UV-C Innactivation WFI

WFI UF | DF Sanitization Buffer Sanitization Buffer Flush Buffer Post-Flush Buffer

Flushing Buffer WFI Storage Buffer Storage Buffer

Washing Buffer Equilibration Buffer Dilution Buffer Dilution Buffer Solvent | Detergent Innactivation Controlled Freeze and ThawDiafiltration Buffer Solvent Buffer Freezing Buffer

Depth Filtration Sanitization Buffer Low-pH Virus Inactivation Membrane Adsoption AEX Flush Buffer WFI

WFI Acid Sanitization Buffer

Post-Flush Buffer Base Flushing Buffer UF | DFDilution Buffer Equilibration Buffer WFI

Post-Flush Buffer Diafiltration Buffer

Strip Buffer Sanitization Buffer

With our downstream offerings the priority is on quality, flexibility, and the assurance that there are no interruptions to supply so your required product is there when you need it, in your preferred format.

The process begins with approved and specified raw materials obtained from qualified vendors. Our insistence on superior-quality materials includes complete NAO (non-animal origin) traceability and our Water for Injection (WFI) systems meet both US and European Pharmacopoeia standards. Standard testing on finished media products includes tests for sterility, osmolality, pH, and endotoxin content.

Global manufacturing facilities follow cGMP (21CFR820) and ISO (9001:2008) regulatory protocols to ensure a consistent, reliable supply of your powdered and liquid media.

Every product we manufacture is backed by the expertise of our professional customer service, technical service and field representatives. Our staff has been trained to meet your service needs whether they are in custom packaging, specialized delivery, follow-up or consulting. We will help our clients match their formulations with the packaging they need to fit their unique system. We can also aid in addressing our client's specific inventory needs and issues. From the time of purchase to the day of shipment, our team will stand by our clients and their product.

Buffer quality systems

56

Downstream Steps

Buffer solutions are available in both liquid or powder formats and a corresponding range of convenient packaging options. Whether you need a standard off-the-shelf buffer or a customized solution, Sartorius Lonza has an experienced team to help guide your selection and development.

Buffer Packaging and Options

The Processk Transfer the pre-weighted dispensing bags or liner

in the mixing room (they are wrapped in overlay to facilitate access to class 8 room)

k Add the desire water quantity to get a required volume of buffer

k Check the pHk Filter as recommended in the protocol

The Benefitk Do not have to purchase individual raw materials

and store itk Do not have to qualify raw material suppliers and

run QC/QA test of new raw materials batchesk Will not have to weigh the powder at the desired

quantityk Will not have to mix the various components of

the buffers in a container to be transported in your mixing room

k Will not have to adjust pH (just validate it)k Will not have to define a filtration protocol

(it will be provided to you)

k A custom buffer composition at your exact specification

k pH pre-adjustedk Powder quantity adjusted to the exact quantity

required for your desired liquid volumek Fully qualified raw material

k Buffer in powder format from 100 g bottles to 20 KKgs drums, for you to weigh, rehydrate, adjust pH and filter in your premises

Enhanced Buffer Formatsk Powder buffers ready for rehydratation either:– Packaged in dispensing bags to be used with

closed mixing system– Or packaged in “liners” for mixing drums to be

used with an open mixing solution

k Ready-to-use liquid buffers manufactured under cGMP (21CFR820) and ISO (9001:2008) available in bottles from 0,5 L to 5 L as well as in bags from 1 L to 1000 L

The Traditional Offering

NEW!

57

Buffers

Buffer Powder in Liners for Open Drum Mixing Solutions

Pre-weighed, pre pH-adjusted powder buffer in a liner

Liner | bag for powder transportation:– Connection to common mixing systems– Tubings for sterile filtration and final fill– Predetermined pH and osmolarity

Installation of the liner into a mixing drum and removal of the clamp

Installation of the liner into a mixing drum and removal of the clamp

Final Storage Bag for the sterile buffer

Powder Bag:– Connection to Lonza Filling Station– Robust for transportation– Connection to common mixing Systems

Closed Mixing System:– Semi-sterile preparation of the medium– No pH adjustment + osmolarity

Optional transfer to storage bag

Storage bag if required:– Customer decision– Quality control of the

final product (sterility, pH, osmolarity, conductivity, endotoxin)

Buffer Powder in Dispensing Bags for Closed Mixing Solution

Features of pre-weighed buffer in dispensing bags and liners:Reduce QA for raw material inspection as well as finished goods k Risk avoidance and cost saving

No powder weighing steps k No need of weighing room, saves time and reduces errors

Complete solution =Full re-suspension | pH and osmolarity adjustment | filtration protocol provided

k Result consistency

Less dust or no dust k Safer

Fully compatible solution for either open drum mixing or closed mixing systems k Ease of use

Easy to store k Storage at ambient | lower storage space up to the mixing step

Same buffer available in liquid (0,5 L to 10 L) or in small drums k Consistency from early PD to large manufacturing scale

58

Phosphate Buffered Salines

Cat. No. Size Without Phenol Red Without Calcium or Magnesium With Hepes

17-516F 500 mL • •

17-516Q 1 L • •

17-517Q 1 L • •

17-737E 100 mL •

17-737F 500 mL •

BE02-017F 500 mL

12-624E 100 mL

17-711E 100 mL •


Storage 15ºC-30ºC

Downstream Steps



17-517Q BE17-517Q PBS-10x w/o Ca, Mg 1 L 1L

17-516Q/12 BE17-516Q/12 PBS-1X w/o Ca, Mg 12 × 1 L 12 × 1 L

17-516F/12 BE17-516F/12 PBS-1X w/o Ca, Mg 12 × 500 mL 12 × 500 mL

17-516Q BE17-516Q PBS-1X w/o Ca, Mg 1 L 1 L

17-516F BE17-516F PBS-1X w/o Ca, Mg 500 mL 500 mL

17-516F/24 17-516F/24 PBS-1X, w/o Ca, Mg 24 × 500 mL 24 × 500 mL

BE02-017F BE02-017F PBS-EDTA 500 mL 500 mL

59

Buffers

Dulbecco’s Phosphate Buffered Saline

Cat. No. Size Without Phenol Red With Calcium and Magnesium With Glucose With Sodium Pyruvate

17-513F 500 mL • •

17-513Q 1 L • •

17-512F 500 mL •

17-512Q 1 L •

04-479Q 1 L • • • •

17-515F 500 mL (10 ×) •

17-515Q 1 L (10 ×) •


Storage 15ºC-30ºC



BE15-512D BE15-512D STD DPBS Powder w/o Ca, Mg 10 L 10 L

N/A BE15-512F STD DPBS Powder w/o Ca, Mg 50 L 50 L

N/A BE15-512K STD DPBS Powder w/o Ca, Mg 1000 L 1000 L

17-512Q/12 BE17-512Q/12 DPBS 0.0095M(PO4) w/o Ca and Mg, 12 × 1 L 12x1 L

17-512Q BE17-512Q DPBS 0.0095M(PO4) w/o Ca and Mg, 1 L 1 L

17-512F BE17-512F DPBS 0.0095M(PO4) w/o Ca and Mg, 500 mL 500 mL

17-512F/12 BE17-512F/12 DPBS 0.0095M(PO4) w/o Ca&Mg, 12 × 500 mL 12x500 mL

17-515Q BE17-515Q DPBS-10x w/o Ca, Mg 1 L 1 L

17-515F BE17-515F DPBS-10X w/o Ca, Mg 500 mL 500 mL

04-479Q 04-479Q DPBS-1X w/ Ca & Mg Glucose and Pyr, 1 L 1 L

17-513Q BE17-513Q DPBS-1X w/ Ca, Mg 1 L 1 L

17-513F BE17-513F DPBS-1X w/ Ca, Mg 500 mL 500 mL

N/A BE17-513F/12 DPBS-1X w/ Ca, Mg BOX of 12 bottles 12 × 500 mL

17-512F/24 17-512F/24 DPBS-1X w/o Ca++, Mg++ 24 × 500 mL 24 × 500 mL

60

Balanced Salt Solutions

Earle’s Balanced Salt Solution

Hank’s Balanced Salt Solution

Cat. No. Size With Phenol Red With Calcium and Magnesium

10-508F 500 mL • •

10-508Q 1 L • •

10-543F 500 mL •

10-543Q 1 L •

10-527F 500 mL •

10-547F 500 mL

04-315Q 1 L



Storage 15ºC-30ºC

Storage 15ºC-30ºC

Downstream Steps



08-003A 08-003A STD HANKS’ BSS, W/O CA,MG OR PR, 4 L BG 4 L

10-527F/12 10-527F/12 HANKS’ BSS, W/O Phenol Red 12 × 500 mL 12 × 500 mL

10-508Q 10-508Q Hanks’ BSS, with Phenol Red, 1 L 1L

10-508F BE10-508F HBSS w/ Phenol Red 500 mL 500 mL

10-543F BE10-543F HBSS w/ PR w/o Ca, Mg 500 mL 500 mL

N/A BE10-543F/12 HBSS w/ PR w/o Ca, Mg BOX of 12 bottles 12 × 500 mL

10-527F BE10-527F HBSS w/o Phenol Red 500 mL 500 mL

10-547F BE10-547F HBSS w/o PR, Ca or Mg 500 mL 500 mL

04-315Q 04-315Q HBSS, w/o Ca++,Mg++ or Phenol Red, 1 L 1 L

10-543Q 10-543Q HBSS, w/o Ca++,Mg++ w/ phenol red, 1 L 1 L


N/A BE02-027F EBSS w/ HEPES low NaHCO3, 500 mL 500 mL

N/A BE10-502F EBSS w/ Phenol Red 500 mL 500 mL

61

Buffers

HEPES BufferOrdering Information


Other Buffers

Storage 15ºC-30ºC


BE17-737E 17-737E HEPES Buf,1M Stk in normal saline, 100 mL 100 mL

17-737F 17-737F HEPES Buffer,1M in normal saline, 500 mL 500 mL


12-747F 12-747F UltraSaline A without Phenol Red, 500 mL 500 mL

10-539B 10-539B Dextrose Gelatin Veronal Buffer, 500 mL 500 mL

12-624E 12-624E Veronal Buffer (5X) 100 mL 100 mL

17-711E BE17-711E Versene (EDTA) 0.02% 100 mL 100 mL

Citrate buffer

Acetate Buffer

Potassium phosphate buffer

Glycine buffer

D Glucose buffer

Tris buffer

Ammonium Sulfate

Sodium Hydroxide

Histidine

Sodium chloride

62

Buffers

WFIDefinitionOur off-the-shelf WFI products are available in 500 mL or 1 L bottles, 20 L and 200 L bags. We can also provide made to-order WFI in custom packaging options to fit your application or direct ready-to-use solutions (Cell Culture Media, Buffers, Organ Transplant Solutions). Delivery time: 24 hours to 1 week from reception of Purchase Order (Add an extra week for Asia). Facilities follow cGMP 21CFR820 and are certified ISO9001 and ISO13485.

Product DescriptionWater For Injection produced in bulk; meeting USP and EP Pharmacopeia criteria; filtered using Sartopore II filters into primary packaging (bottles and | or bags)– Available in 500 mL or 1 L bottles, in 20 L or 200 L Bags off

the shelf

Bulk packaged for commercial use elsewhere, not for parenteral administration. Our WFI Quality Water is aseptically processed from a validated Water For Injection System that meets current USP and EP specifications in the loop. Our WFI Quality Water is not to be confused with Sterile Water for Injection which is intended for use in extemporaneous prescription compounding, and as a diluent for injectable parenteral products. In addition, Sterile Water for Injection must be packaged in single dose containers not larger than 1 liter in size.

This product is manufactured aseptically under cGMP 21CFR820 conditions by a validated sterile filtration method and tested according to current U.S.P. and E.P. This product has been produced for laboratory | manufacturing use. Use for in vitro diagnostic procedures has not been established. It is the end user’s responsibility to ensure that the final products meets the requirements of the application.

FeaturesVery Low Endotoxin level and

Sterilek Minimization of Contamination

High Quality made under cGMP

21CFR820 conditionsk Regulatory Friendly and Risk

Mini mization

No need for internal installation of

WFI loopk Significant Savings

ApplicationSubject to the proviso, customer checks quality according to CoA for suitability to his | her needs UpStream: Media Preparation and HydrationDownstream: Buffer PreparationUpStream & Downstream: Cleaning, Washing, Rinsing (filters, equipment)




08-199D N/A STD WFI Comm. Use Elsewhere, 100 L bag 100 L

08-199E N/A STD WFI Comm. Use Elsewhere, 200 L drum 200 L

08-199ES N/A STD WFI Sample Bag – 2 L bag 2 L

08-199F N/A STD WFI Comm. Use Elsewhere, 20 L bag 20 L

N/A BE02-038P0.5 Bulk Packaged ster.filtered WFI – 500 mL 500 mL

N/A BE02-038P20 Bulk Packaged sterile filtered WFI-20 L 20 L

N/A BE02-038P200 Bulk Packaged sterile filtered WFI-200 L 200 L

17-724Q BE17-724Q USP WFI bulk Sterile filtered 1 L 1 L

17-724F BE17-724F USP WFI bulk Sterile filtered 500 mL 500 mL

N/A BE17-724F/12 WFI Water-Cell Culture BOX of 12 bottles 12 × 500 mL

N/A BE17-724Q/12 WFI Water-Cell Culture BOX of 12 bottles 12 × 1 L

Reagents

ReagentsThe Sartorius Lonza Cell Culture Reagents portfolio includes products such as amino acids, antibiotics, and assorted processing agents. These products, which can be manufactured under the same cGMP conditions as our other products, are routinely used in both research and manufacturing applications.

Chemicals used to prepare cell culture reagents are purchased according to raw material qualifications from approved suppliers. Each lot must meet established component specifications before Quality Assurance release. All liquid cell culture reagents are manufactured using Water for Injection (WFI) quality water and all liquid products are filtered through pharmaceutical grade sterilizing filters. If there is a desired cell culture reagent that is not listed in our standard portfolio, we can create a custom reagent specific for your requirements.

Sodium bicarbonate p.64

Sodium pyruvate p.64

Insulin p.65

Trypsin p.65

L-Glutamine p.66

Ultra Glutamine p.66

Penicillin p.67

Amphotericin p.68

Gentamycin p.68

ProFreeze™ p.69

Anticlumping Agents p.70

64

Reagents

Buffering Solutions or Chemicals

Sodium Bicarbonate

Sodium Pyruvate



Storage 15ºC-30ºC

Storage 2ºC-8ºC


15-613I BE15-613I STD Sodium Bicarbonate powder 500 g 500 g

17-613E BE17-613E Sodium Bicarb. 7,5% sol 100 mL 100 mL


13-115E BE13-115E Sodium Pyruvate Solution 100 mM, 100 mL 100 mL

65

Reagents

Amino-Acids, Peptides, Proteins & Enzymes

Insulin

Trypsin



Storage -10ºC--20ºC



02-033E BE02-033E STD Insulin Human recomb. 5 mg/mL 100 mL 100 mL

02-033E20 BE02-033E20 STD rh Insulin, 5 mg/mL, 20 mL bottle 20 mL

02-033Q N/A STD Human Recombinant Insulin 5 mg/mL, 1 L 1 L

BE02-033E80 N/A Human Rec Insulin 5 mg/mL 80 mL 80 mL


17-160F 17-160F Trypsin(10 +) 2.5% /HBSS w/o Ca, Mg, 500 mL 500 mL

BE02-007E BE02-007E Trypsin/EDTA 10 × 100 mL 100 mL

N/A BE02-034E Trypzean/EDTA solution 100 mL bottle 100 mL

17-160E BE17-160E Trypsin(10 +) 2.5% in HBSS w/o Ca, Mg 100 mL 100 mL

N/A BE17-161E/12 Trypsin-Versene (EDTA) 12 × 100 mL 12 × 100 mL

17-161E BE17-161E Trypsin-Versene (EDTA) Mix (1 +) 100 mL 100 mL

17-161F BE17-161F Trypsin-Versene (EDTA) Mix (1 +) 500 × mL 500 mL

66

Reagents

Vitamins & Other Nutrients

Ultra Glutamine

L-GlutamineOrdering Information



Storage 15ºC-30ºC


15-605G BE15-605G L-GLUTAMINE POWDER 25 G 25 g

N/A BE15-605X(3000) STD L-Glutamine Powder 3000 g 3000 g

17-905C 17-905C L-Glutamine 200mM Hybridoma test, 50 mL 50 mL

17-605E BE17-605E L-Glutamine 200mM 100 mL 100 mL

17-605F BE17-605F L-Glutamine 200mM 500 mL 500 mL

17-605C 17-605C L-Glutamine 200mM 50 mL 50 mL

N/A BE17-605E/12 L-Glutamine 200mM BOX of 12 bottles 12 × 100 mL


BE17-605E/U1 BE17-605E/U1 Ultraglutamine I 200mM 100 mL 100 mL

N/A BE17-605E/U1/12 Ultraglutamine I 200mM BOX of 12 bottles 12 × 100 mL

N/A BE04-684E UltraGlutamine II 100 mL 100 mL

N/A BE04-684E/12 UltraGlutamine II BOX of 12 bottles 12 × 100 mL

67

Reagents

Antibiotics

Cat. No. Size 5,000 Units Penicillin

–5,000 μg Streptomycin

10,000 Units Penicillin


20,000 Units Penicillin


25,000 Units Penicillin –

25,000 μg Streptomycin

25 μg/mL

Amphotericin B

With

L-glutamine

17-603E 100 mL •

17-602E 100 mL •

17-602F 500 mL •

17-718R 25 ×

4.5 mL

• •

09-757F 500 mL •

17-745H 20 mL • •

17-745E 100 mL • •

Related Products Page

MycoZap™ Antibiotics 164

MycoAlert™ PLUS Mycoplasma Detection Kit 161

Penicillin-Streptomycin Mixtures


Related Products



17-602E DE17-602E Pen 10.000 UI/mL Strep 10.000 UI/mL 100 mL

17-602F DE17-602F Pen 10.000 UI/mL Strep 10.000 UI/mL 500 mL

17-603E DE17-603E Pen 5.000 U/mL Strep 5.000 U/mL 100 mL

09-757F 09-757F Pen/Strep stock, 20K/20K 500 mL 500 mL

17-745E 17-745E Pen/Strep/Fungizone 10K/10K/25 μg, 100 mL 100 mL

17-745H 17-745H Pen/Strep/Fungizone 10K/10K/25 μg, 20 mL 20 mL

17-718R 17-718R Pen/Strep/L-Gln 25K/25K/200 mM, 25 + 4.5 mL 25 + 4.5 mL

N/A DE17-603E/12 Penicillin/Strep 5000U/mL BOX of 12 BOT 12x100 mL

N/A DE17-602E/12 Penicillin10.000 UI/mL BOX of 12 bottles 12x100 mL

68

Reagents

Antibiotics

AmphotericinOrdering Information

GentamicinOrdering Information


Storage 15ºC-30ºC


17-836R 17-836R Amphotericin B, antifungal 250 ug/mL, 20 mL 20 mL

17-836E 17-836E Amphotericin B, antifungal 250ug/mL, 100 mL 100 mL


17-519Z 17-519Z Gentamicin 10 mg/mL 1 × 10 mL screw cap 10 mL

17-519L 17-519L Gentamicin 10 mg/mL 10 × 10 mL screw cap 10 × 10 mL

17-528Z 17-528Z Gentamicin 50 mg/mL 1 × 10 mL crimp-top 10 mL

17-518Z 17-518Z Gentamicin 50 mg/mL 1 × 10 mL screw cap 10 mL

17-518L 17-518L Gentamicin 50 mg/mL 10 × 10 mL screw cap 10 × 10 mL

N/A BE02-012E Gentamycin Sulfate 100 mL, 10 mg/mL Gent 100 mL

69

Reagents

Additives & Freezing Media

ProFreeze™ CD (2X), NAO Freeze Medium Chemically Defined, Non-animal Origin Freezing Medium

ProFreeze™ CD (2X), NAO Freeze Medium – Chemically Defined is universally suitable for cryopreserving many cell types in the absence of fetal bovine serum (FBS). However, it is used to greatest advantage with cells cultured in a serum-free and animal component-free environment. This protein-free freezing medium contains no animal derived components, insulin, or hydrolysate, and maintains high cell viability upon recovery from frozen storage.ProFreeze™ Medium requires the addition of 15% reagent or spectrophotometric grade dimethylsulfoxide (DMSO) at time of use. One bottle will make 117.6 mL of complete 2X concentrated freezing medium after the addition of 17.6 mL DMSO. For best results, keep on ice during use.


Storage 2ºC-8ºC



BP12-769E BP12-769E ProFreeze-CDM, NAO, 2x Freeze Med, 100 mL 100 mL

70

Reagents

Additives & Freezing Media

Anti-Clumping Agents

Chinese Hamster Ovary (CHO) cells are widely used in large-scale culture. These cells have conventionally been grown as attached cultures, but they will also grow in suspension, a property which has allowed for relatively simple scale up in stirred tank and airlift bioreactors. Unfortunately, some CHO-derived lines which have been adapted to suspension growth can form large, tightly bound aggregates in culture.

Why is clumping a problem?– For batch suspension cultures, cell aggregation hinders accurate cell counting, monitoring and control of the cellular environment– Transport of nutrients to, and products from, the cells may be impaired– Cluster formation dramatically influences the growth behavior of the cells– Cells within aggregates show a strongly reduced specific proliferation rate– Shear forces exerted on large aggregates cause a considerably higher specific death rate than those exerted on single cells, reducing

the specific growth rate up to 50 %

Approaches to solving the problem of cell aggregation have proven to be less than satisfactory, until now… Lonza introduces Anti-clumping Agents A and B, two ready-to-use solutions designed to minimize CHO cell clumping in suspension cultures. Both solutions are made exclusively of non-animal origin components.

– Anti-clumping Agent A is a buffered, concentrated solution of Dextran sulfate, and recommended usage is at 100 × dilution– Anti-clumping Agent B is a buffered, concentrated solution of ferric citrate, and recommend use it at 100 × or 200 × dilution

As a result of using Anti-clumping Agents from Lonza, clumping of cells cultured in serum-free medium is minimized, leading to a significant increase in cell density and productivity.

Control image shows clusters of CHO CRL-11397 cells grown in suspension culture.

CHO CRL-11397 cells, grown with Anti-clumping Agent A (Dextran sulfate),do not aggregate in suspension culture.

CHO CRL-11397 cells, grown with Anti-clumping Agent B (Ferric citrate), do not aggregate in suspension culture.


71

Reagents

Resolution of Clumping Problems in CHO Cells Leads to a Signicant Increase in the Cell Density and Productivity

Units/m

L

0 0

5,0000.2

0.4

1.4

1.6

1.8

2.0

0.6

0.8

1.0

1.2

15,000

10,000

20,000

25,000

30,000

35,000

Avg.

RFU

PowerCHO™ 3(12-772Q)

Day 3 – growth Day 4 – growth Day 5 – growth Day 6 – growth Day 7 – prod

FeCit – 1/200(BERDO48E)

FeCit – 1/100(BERDO48E)

Dextran Sulfate – 1/100(BERD047E)


As noted in the graph, the effect of both agents on CHO cells seeded in PowerCHO™ 3 Medium is obvious. Cell density is significantly increased by the addition of Anti-clumping Agent B after just three days in culture, while, after seven days on culture, both Anticlumping Agents A and B exhibit increased productivity of 20 up to 35 %.


BE02-058E BE02-058E STD Anti clumping agent A 100 mL 100 mL

Store at 15° – 30° C

72

Services

Media Services

In addition to a broad standard classical and chemically-defined product portfolio, the Sartorius Lonza media services team is able to provide cell culture solutions specific to a client’s unique processing needs. These services leverage the extensive knowledge base of our commercial, regulatory, and scientific experts and are available across the entire spectrum of bioprocessing unit operations (USP/DSP/FF), formats (liquid/powder), and clinical applications.

Media ServicesCustomization p.74

Support p.74

Validation p.74

Quality p.76

Packaging and Shipping p.80

Services

Sartorius Lonza will work collaboratively with clients to produce unique media solutions. This service option is production-focused and the perfect choice for clients who have known, desired media composition and outcome expectations. Additionally, clients may request specific suppliers for critical raw materials or rely upon Sartorius Lonza’s established global network of qualified suppliers. Sartorius will follow the clients requested parameters and project scope. It is also possible to request customized packaging, media formats, and release assays.

Customization

ValidationProducts that are supplied for further manufacturing use are manufactured in qualified and validated equipment. Most standard catalogue items have a specific shelf life indicated on the Certificate of Analysis. For products that do not have shelf life data (custom products), we offer shelf life studies (standard or accelerated) on request.Other services like filtration studies, leachable | extractable studies, can be offered with support from our Confidence® group.

The combined resources of the Sartorius | Lonza collaboration allow for a dedicated team of media professionals with a broad range of expertise including R&D, clinical manufacture, product commercialization, engineering, and media development. Certificates of Analysis (C of A’s) for standard products are available online at http://www.lonza.com/coaTechnical questions on standard media products can be directed to Scientific Support at [email protected]

Custom and specialized media projects will be assigned a project team composed of a Sales Representative, a Media Project Manager, and a Media Application Specialist.

Support

75

Services

Services

Quality

Sartorius’ manufacturing partners, Lonza Walkersville, Inc. and Lonza Verviers, Inc. are currently engaged in the manufacture of over 1,500 products for pharmaceutical and biotechnology applications such as disease diagnosis, biomedical research, production of diagnostics and therapeutics, and quality control testing of pharmaceuticals and medical devices for the presence of bacterial contaminants.Manufacture of these products, specifically cell culture media, reagents, and buffers, follow the protocols and compliance specifications of the following industry regulations:– 21CFR820– ISO 9001: 2008 certified– ISO 13485: 2003 certified

Further information on these protocols and document management are available upon request. Quality agreements and audits are available at the customer’s discretion.

USP Sterility – Tests are performed on representative samples using the membrane filtration procedure in accordance with the US Pharmacopoeia or EP Pharmacopoeia. Culture media used are fluid thioglycollate medium (FTM) and trypticase soy broth (TSB). All tested media must pass growth promotion testing before use. The test samples are filtered and the filters are immersed in FTM and TSB. TSB cultures are incubated at 22.5°C +/- 2.5°C. FTM cultures are incubated at 32.5°C +/- 2.5°C. The cultures are incubated for 14 days, during which they are periodically examined and sterility results are recorded.

Moisture Content –This is a powder specific test. We are committed to offering the same high quality powdered media as you have come to expect with our liquid media. We utilize a unique micronization manufacturing process, which produces powder in a controlled environment, guaranteeing maximum stability and minimum degradation due to chemical oxidation.

Endotoxin – Products are tested for endotoxin content using the Kinetic-QCL™ Assay. Test dilutions used are screened for inhibition and enhancement in the Kinetic-QCL™ Assay. Endotoxin levels for these products are available on Certificates of Analysis.

Available final product testing includes the following:

Cell Growth Promotion – Media and supplements are tested for their growth-promoting properties using primary, diploid, and heteroploid cell lines unless other cells are appropriate based on the product use. Results are obtained using the Lowry protein method or manual cell count to assess cell replication. Visual screening of cultures is also done to ensure characteristic cell morphology and lack of toxicity. Additional testing for toxicity, function and/or activity may also be conducted if one or more is required in the product release specifications.

Chemistries (pH and Osmolality) – Tests are performed on representative samples from each lot using routinely calibrated equipment. Osmolality is determined by means of the highly repeatable freezing point method.

Shelf Life –Samples from each lot of standard product are retained and stored at label temperature in the event follow-up testing is required. Subsequent testing may be in response to your inquiry or for shelf life studies. Shelf life data is not included but is available for some products and upon request. Shelf life is generally between 6 and 24 months for liquid products and 24 months for powder products.

76

Quality

Release Testing and Documentation

Liquid media Powder media

Testing Options

Sterility Standard N/A

pH Standard N/A

Osmolality Standard Standard

Endotoxin Standard N/A

Cell Growth Promotion Standard on CHO medias N/A

Mycoplasma On Request N/A

Moisture N/A Standard

Bioburden N/A Standard

Shelf Life On Request On Request

Documentation

CofA and raw material CoA Standard Standard

In order to maintain consistent quality in sterile cell culture media products, strict control of each production lot is essential. Written procedures in accordance with current Good Manufacturing Practices (cGMPs) provide quality control from start to finish for each product.

To verify the conformity of material to established specification throughout the manufacturing process, inspections and testings occur during the following four stages: – Raw material receipt– In-process manufacturing, labeling and packaging– Finished product– Post-manufacture for shelf life studies.

77

78

Services

Attributes Product for research Non-GMP (“Pilot”) catalogue products and custom Protein | Vaccine production media for cGMP production

Product use For research use onlyFor upstream | downstream biopharma process development

For upstream | downstream Biopharma clinical phase 3 or commercial production

Quality Assurance

QA System ISO 9001 ISO 9001, ISO 13485 ISO 9001, ISO 13485 and cGMP 21CFR821DHR–Device History Record – – h

PI Sheet–Process Instruction Sheet (in SAP) – – h

MSDS–Material Safety Data Sheet h h h

CoA– Certificate of Analysis h h h

TSE | BSE statement On request h h

CoO– Certificate of Origin On request h h

Master Table– Theoretical data with all approved sources. – On request h

Batch Table– Production real data – On request h

Specification sheet– Internal Testing References N.A. N.A. –

Labels Request (for custom products) N.A. N.A. h

CPR– Custom Product Request N.A. h h

Change Notification None Systematic and proactive (timelines 6 weeks) Systematic and proactive (timelines 6 weeks)

Quality Control Product Approval and Release No QA inspection of documentsAll products are released following an approved | controlled an inspection of all quality documents harmonized between both sites

All products are released following an approved | controlled QA inspection of all quality documents harmonized between both sites

Product Storage and Shipment

Shipment conditions Theoretical shipment conditionsHarmonized shipping condition between sites supported by validation data

Harmonized shipping condition between sites supported by ASTM validation data

Handling of customer demands

Handling of Customer Quality Agreements N.A. 21 day Lonza Turnaround Time 21 day Lonza Turnaround TimeCustomer Complaints N.A. 7 day Lonza Turnaround Time 7 day Lonza Turnaround Time

Handling of Customer Audits N.A.

Upon receipt of customer’s audit request Lonza/Sartorius will respond with 3 potential dates all within a period of 3 months from request date –> Any corrective action response required as an audit outcome, a receipt notofication will be send within 5 open days and a corrective action plan (CAPA) is expected to be answered within 60 days

Upon receipt o customer’s audit request Lonza | Sartorius will respond with 3 potential dates all within a period of 3 months from request date –> Any corrective action response required as an audit outcome, a receipt notofication will be send within 5 open days and a corrective action plan (CAPA) is expected to be answered within 60 days

Confidentiality N.A. Confidential information shared under CDA Confidential information shared under CDA

Quality Charter

79

Quality

Attributes Product for research Non-GMP (“Pilot”) catalogue products and custom Protein | Vaccine production media for cGMP production

Product use For research use onlyFor upstream | downstream biopharma process development

For upstream | downstream Biopharma clinical phase 3 or commercial production

Quality Assurance

QA System ISO 9001 ISO 9001, ISO 13485 ISO 9001, ISO 13485 and cGMP 21CFR821DHR–Device History Record – – h

PI Sheet–Process Instruction Sheet (in SAP) – – h

MSDS–Material Safety Data Sheet h h h

CoA– Certificate of Analysis h h h

TSE | BSE statement On request h h

CoO– Certificate of Origin On request h h

Master Table– Theoretical data with all approved sources. – On request h

Batch Table– Production real data – On request h

Specification sheet– Internal Testing References N.A. N.A. –

Labels Request (for custom products) N.A. N.A. h

CPR– Custom Product Request N.A. h h

Change Notification None Systematic and proactive (timelines 6 weeks) Systematic and proactive (timelines 6 weeks)

Quality Control Product Approval and Release No QA inspection of documentsAll products are released following an approved | controlled an inspection of all quality documents harmonized between both sites

All products are released following an approved | controlled QA inspection of all quality documents harmonized between both sites

Product Storage and Shipment

Shipment conditions Theoretical shipment conditionsHarmonized shipping condition between sites supported by validation data

Harmonized shipping condition between sites supported by ASTM validation data

Handling of customer demands

Handling of Customer Quality Agreements N.A. 21 day Lonza Turnaround Time 21 day Lonza Turnaround TimeCustomer Complaints N.A. 7 day Lonza Turnaround Time 7 day Lonza Turnaround Time

Handling of Customer Audits N.A.

Upon receipt of customer’s audit request Lonza/Sartorius will respond with 3 potential dates all within a period of 3 months from request date –> Any corrective action response required as an audit outcome, a receipt notofication will be send within 5 open days and a corrective action plan (CAPA) is expected to be answered within 60 days

Upon receipt o customer’s audit request Lonza | Sartorius will respond with 3 potential dates all within a period of 3 months from request date –> Any corrective action response required as an audit outcome, a receipt notofication will be send within 5 open days and a corrective action plan (CAPA) is expected to be answered within 60 days

Confidentiality N.A. Confidential information shared under CDA Confidential information shared under CDA

Services

Individually, our Business Units are industry-leading suppliers for the application they serve, such as filtration, fluid handling and fermentation. Combining forces collectively enhances our strength in production while reinforcing Sartorius Stedim Biotech | Lonza as a global supplier for the biopharmaceutical industry. We can therefore assist in designing and providing the best products for our customers.With decades of experience and a rich heritage in engineering, we offer packaging solutions answering your specific needs providing excellence in customer service, product quality and innovation solutions.We want to protect our products in the best possible way with high-quality packaging – on the inside as well as the outside. We always place particular value on the highest standards of safety and security, in order to offer efficient and flexible solutions in a market that is both challenging and constantly changing.

Leveraging Sartorius and Lonza Strengths

Packaging

Media & Buffer product storage and shipping conditions vary. While some can be stored at ambient temperature (uncontrolled), others might need to be stored at Room Temperature (15-30°C), Refrigerated Temperature (2-8°C) or Frozen Temperature (-10°C/-20°C).

Appropriate packaging is provided to maintain optimal shipping conditions.We have years of experience in shipping small volumes as well as large volumes of our products (in liquid or powder form) at different temperatures all around the globe. Dedicated transport can be offered at specific costs. Please contact your Sartorius representative or Media & Buffer Specialist for further information.

Shipping

Features and Benefits Skilled staff, a careful choice of materials, pioneering infrastructure, efficient internal process management and a transparent communication policy guarantee reliability, delivery times, the highest quality standards and strict cost control.

Liquids With a comprehensive range of bottles and bags we can offer standard off-the-shelf or customized containment from 0.5 liters up to 1000 liters.

Powders We offer a wide range of powder containers from 10g bottles up to several 100 kg. We also provide ready-to-use solutions such a pre-weighted powder in bags, media kits, etc.

Industry leader in batch size scalability

k Liquid: 0.5 L to 10,000 L batch sizePowder: 7 kg to 7,000 kg batch size

Dual global manufacturing sites

k Assurance of supply

Sartorius Lonza k Fit-for-purpose and ready to use solutions adding value to your processes

Wide range of primary and secondary packaging

Answering your specific needs

80

81

Shipping & Packaging

Shipping Solutions for Media & Buffers | Liquid Form

0,5 to 1 L 1 to 2,5 L 2 to 20 L 50 L 100 L 200 L 500 L 1000 L

Bottle h h

LDPE 2D Pillow Bag h h

Drum h h h

LDPE 3D Bag in Container h h h h

Stainless Steel Palletank h h h h

Plastic Palletank h h

82

Packaging

Shipping Solutions for Media & Buffers | Powder Form

10 g to 2 kg 5 kg to 20 kg 5 kg to 20 kg 2 kg to 20 kg 500 g to 2 kg

Bucket h

Drum h

Powder Bag h h

Flexel Tankliner h

Applicable for Media & Buffers

Media & Buffers

Media & Buffers

NEW!

Buffers (pre-weighted

solution for closed mixing

systems)

NEW!

Buffers (pre-weighted

solution for open mixing

systems)

Classical Media

Classical MediaClassical media are produced using well-known public recipes (published formulations). Nevertheless, Lonza manufactures this type of media with great care, following cGMP guidelines and in accordance with ISO:9001. A Device Master Record (DMR) is prepared for every medium produced. It defines the production procedures–from receipt of raw materials to final product release, the environmental and processing controls required, as well as product specifications (including packaging and labeling), ensuring batch-to-batch consistency. Like all other BioWhittaker™ Media, classical media are manufactured with the highest-grade raw materials, most often EP or USP compliant and sourced from qualified and approved suppliers. Water for Injection (WFI) quality water is consistently used to produce any liquid media product. Indeed, we rely on our excellence and expertise to offer you the safest, most reliable and most consistent products.

BME (Basal Medium Eagle)

p.84

DMEM (Dulbecco’s Modified Eagle’s Medium)

p.84

EMEM (Eagle Minimum Essential Medium)

p.86

DMEM:F12 Medium p.88

GMEM (Glasgow Minimum Essential Medium)

p.88

Grace’s Insect p.89

Ham’s p.89

IMDM (Iscove’s Modified Dulbecco’s Medium)

p.90

L-15 (Leibovitz) p.90

M199 p.91

Mc Coy’s p.91

PC-1™ p.92

HL-1™ p.94

RPMI p.96

William’s E p.97

Richter’s p.97

NCTC-109 p.97

UltraMEM™ p.98

84

Classical Media

Basal Medium Eagle (BME)A minimal medium suitable for a variety of cell types. It is a historical precursor to Minimum Essential Medium (MEM).


BE12-105F BE12-105F Basal Medium Eagle (BME) With Earle’s BSS, without L-glutamine 500 mL


DMEM is used in a wide range of mammalian cell culture applications. The high glucose version is well suited to high density suspension culture. The low glucose formula is used for adherent dependent cells.

Dulbecco’s Modified Eagle’s Medium (DMEM)

Cat. No. Size Without

L-Glutamine

Without Sodium

Pyruvate

Without

PhenolRed

With HEPES With 1.0g/L

glucose

With 4.5g/L

glucose

Powder Hybridoma

Screened

With UG I*

12-614F 500 mL • • •

12-614Q 1 L • • •

12-604F 500 mL • •

12-604Q 1 L • •

BE12-604F/U1 500 mL • • • •

12-914F 500 mL • • • •

12-917F 500 mL • • • •

12-707F 500 mL • • •

12-708F 500 mL • • • •

12-709F 500 mL • • • •

12-733F 500 mL • •

12-733Q 1 L • •

12-741F 500 mL •

* With UltraGlutamine I

Storage 15ºC-30ºC

Storage 2ºC-8ºC



85

Classical Media


12-614F BE12-614F Dulbecco‘s Modified Eagle Medium (DMEM) With 4.5 g/L glucose, without L-Glutamine 500 mL

12-614Q 12-614Q Dulbecco's Modified Eagle Medium (DMEM) With 4.5 g/L glucose, without L-Glutamine 1 L

12-604F BE12-604F Dulbecco‘s Modified Eagle Medium (DMEM) With 4.5 g/L glucose, with L-Glutamine 500 mL

12-604Q 12-604Q Dulbecco's Modified Eagle Medium (DMEM) With 4.5 g/L glucose, with L-Glutamine 1 L

BE12-604F/U1 BE12-604F/U1 Dulbecco‘s Modified Eagle Medium (DMEM) With 4.5 g/L glucose, with UltraGlutamine™ I 500 mL

12-914F BE12-914F Dulbecco‘s Modified Eagle Medium (DMEM) With 4.5 g/L glucose, without L-Glutamine, hybridoma tested 500 mL

12-917F BE12-917F Dulbecco‘s Modified Eagle Medium (DMEM) With 4.5 g/L glucose, without L-Glutamine or phenol red 500 mL

12-707F BE12-707F Dulbecco's Modified Eagle Medium (DMEM) With 1.0 g/L glucose, without L-Glutamine 500 mL

12-708F BE12-708F Dulbecco‘s Modified Eagle Medium (DMEM) With 1.0 g/L glucose, without L-Glutamine, with 25 mM HEPES 500 mL

12-709F BE12-709F Dulbecco's Modified Eagle Medium (DMEM) With 4.5 g/L glucose, without L-Glutamine, with 25 mM HEPES 500 mL

12-733F BE12-733F Dulbecco‘s Modified Eagle Medium (DMEM) With 4.5 g/L glucose, without L-Glutamine or sodium pyruvate 500 mL

12-733Q 12-733Q Dulbecco's Modified Eagle Medium (DMEM) With 4.5 g/L glucose, without L-Glutamine or sodium pyruvate 1 L

12-741F BE12-741F Dulbecco‘s Modified Eagle Medium (DMEM) With 4.5 g/L glucose, with L-Glutamine, without sodium pyruvate 500 mL


86

Classical Media

Eagle Minimum Essential Medium (EMEM) Storage 2ºC-8ºC

Cat. No. Size W/

L-glutamine

W/

Sodium

Pyruvate

W/o

Phenol

Red

W/

HEPES

W/

UG I*

W/

HBSS

W/

EBSS

W/

NEAA

W/

Pen-strep-

amph B

W/o Na-

bicarbonate

W/o

Calcium

W/

Nucleosides

12-169F

(Alpha)

500 mL • • •

BE02-002F

(Alpha)

500 mL • • • • •

12-611F 500 mL • •

12-611Q 1 L • •

12-125F 500 mL •

12-125Q 1 L •

12-662F 500 mL • • •

12-662Q 1 L • • •

12-136F 500 mL • •

12-136Q 1 L • •

12-736E 100 mL • • • • •

12-736F 500 mL • • • • •

12-684F

(10x)

500 mL •

12-668E

(2x)

100 mL • •

06-174G 450 mL • • • •

12-127F 500 mL •

12-137F 500 mL • •

04-719Q

(Joklik’s)

1 L • • •

* With UltraGlutamine I


MEM Eagle is suitable for a diverse spectrum of mammalian cell types. It is available with either Hanks’ or Earle’s salts. MEM-Hanks’ (12-127F or 12-137F) does not require a CO2 enriched atmosphere. Joklik’s modification is intended for suspension culture.

87

Classical Media


12-169F BE12-169F Minimum Essential Medium -

Alpha Eagle with Earle‘s BSS

Without L-Glutamine, deoxyribonucleosides and ribonucleosides 500 mL

BE02-002F BE02-002F Minimum Essential Medium –

Alpha Eagle

With UltraGlutamine™ I, deoxyribonucleoside and ribonucleosides 500 mL

12-611F BE12-611F Minimum Essential Medium –

Eagle with Earle‘s BSS

With L-Glutamine 500 mL

12-611Q 12-611Q Minimum Essential Medium –


With L-Glutamine 1 L



Without L-Glutamine 500 mL

12-125Q 12-125Q Minimum Essential Medium –


Without L-Glutamine 1 L

12-662F BE12-662F Minimum Essential Medium – Eagle Without L-Glutamine, with non-essential amino acids and sodium pyruvate 500 mL

12-662Q 12-662Q Minimum Essential Medium – Eagle Without L-Glutamine, with non-essential amino acids and sodium pyruvate 1 L



Without L-Glutamine, with 25 mM HEPES 500 mL

12-136Q BE12-136Q Minimum Essential Medium –


Without L-Glutamine, with 25 mM HEPES 1 L

12-736E 12-736E Minimum Essential Medium –


Cell culture maintenance medium 12-136 with non-essential amino acids,

L-Glutamine, 25 mM HEPES, 10 μg/mL gentamicin, 50 units/mL penicillin,

50 μg/mL streptomycin, 2.5 μg/mL amphotericin B, and 2.0% heat-inactivated

FBS

100 mL

12-736F 12-736F Minimum Essential Medium –


With L-Glutamine 500 mL


Eagle with Earle‘s BSS (10X)

Without L-Glutamine or NaHCO3 500 mL

12-668E 12-668E Minimum Essential Medium –


Without L-Glutamine or phenol red (virus plaquing medium) 100 mL

BE12-668F BE12-668F Minimum Essential Medium –


Without L-Glutamine or phenol red (virus plaquing medium) 500 mL

06-174G BE06-174G Minimum Essential Medium –


With non-essential amino acids, and L-Glutamine, without calcium 450 mL


Eagle with Hank's BSS

Without L-Glutamine 500 mL

12-137F 12-137F Minimum Essential Medium – Eagle Without L-Glutamine, with 25 mM HEPES 500 mL

04-719Q 04-719Q Minimum Essential Medium – Eagle

Joklik’s Formulation

For suspension cultures, with L-Glutamine, without calcium 1 L


88

Classical Media

DMEM:F12 MediumDMEM:F12 combines the richness of F12 with the higher component concentrations of DMEM. This medium is well – suited for clonal density cultures.

Cat. no. Size Without L-

Glutamine

With HEPES With 3.151g/L

Glugose

Powder With UG I*

12-719F 500 mL • •

12-719Q 1 L • •

BE04-687Q 1 L •

BE04-687F/U1 500 mL • • •


12-719F BE12-719F Dulbecco‘s Modified Eagle Medium:F12

(DMEM:F12)

1:1 mixture with 3.151 g/L glucose, L-Glutamine and 15 mM

HEPES

500 mL

12-719Q 12-719Q Dulbecco‘s Modified Eagle Medium:F12

(DMEM:F12)

1:1 mixture with 3.151 g/L glucose, L-Glutamine and 15 mM

HEPES

1 L

BE04-687F/

U1

BE04-687F/

U1

Dulbecco‘s Modified Eagle Medium:F12

(DMEM:F12)

1:1 mixture with 3.151 g/L glucose, with UltraGlutamine™ I,

without HEPES

500 mL

BE04-687Q BE04-687Q Dulbecco‘s Modified Eagle Medium:F12

(DMEM:F12)

1:1 mixture with 3.151 g/L glucose, with UltraGlutamine™ I,

without HEPES

1 L


Glasgow Minimum Essential Medium (GMEM)Designed to support BHK-21 cells.


12-739F BE12-739F Glasgow Minimum Essential Medium With L-Glutamine 500 mL


Storage 2ºC-8ºC

Storage 2ºC-8ºC


89

Classical Media

Grace’s Insect Medium

Ham’s Medium

Classical insect cell media.

Developed for low density (clonal) growth of CHO cells.


04-457F 04-457F Grace‘s Insect Medium Without FBS, with yeastolate, Lactalbumin hydrolysate or gentamicin 500 mL

04-501Q 04-501Q Grace‘s Complete Insect Medium

(TNM-FH)

With 10% heat-inactivated FBS, yeastolate, Lactalbumin hydrolysate, and

gentamicin

1 L

04-649F 04-649F Grace‘s Insect Medium Without FBS, with yeastolate, Lactalbumin hydrolysate, and gentamicin 500 mL



BE02-014F Ham‘s F10 Medium With UltraGlutamine™ I, without thymidine 500 mL

12-615F BE12-615F Ham‘s F12 Medium With L-Glutamine 500 mL

12-618F BE12-618F Ham‘s F10 Medium With L-Glutamine 500 mL


Storage 2ºC-8ºC

Storage 2ºC-8ºC



90

Classical Media

Iscove’s Modified Dulbecco’s Medium (IMDM)IMDM is suitable for fast growing cells. All formulas contain HEPES for added buffering.

Cat. No. Size Without L-Glutamine With HEPES Hybridoma Screened

12-722F 500 mL •

12-722Q 1 L •

12-726F 500 mL • •

12-726Q 1 L • •

12-915F 500 mL • •


12-722F BE12-722F Iscove‘s Modified Dulbecco‘s Medium (IMDM) With L-Glutamine and 25 mM HEPES 500 mL

12-722Q 12-722Q Iscove‘s Modified Dulbecco‘s Medium (IMDM) With L-Glutamine and 25 mM HEPES 1 L

12-726F BE12-726F Iscove‘s Modified Dulbecco‘s Medium (IMDM) Without L-Glutamine, with 25 mM HEPES 500 mL

12-726Q 12-726Q Iscove‘s Modified Dulbecco‘s Medium (IMDM) Without L-Glutamine, with 25 mM HEPES 1 L

12-915F BE12-915F Iscove‘s Modified Dulbecco‘s Medium (IMDM) With L-Glutamine and 25 mM HEPES, hybridoma tested 500 mL


L-15 (Leibovitz) MediumDeveloped for fast growing tumor cells, this formula does not require a CO2 enriched atmosphere. The bicarbonate free medium is buffered with elevated levels of amino acids.


12-700Q 12-700Q L-15 Leibovitz's Medium Without L-Glutamine 1 L

12-700F BE12-700F L-15 Leibovitz's Medium Without L-Glutamine 500 mL

12-669E 12-669E L-15 Leibovitz's Modified

Medium (2X)

(2X) except L-tyrosine (1X), without L-Glutamine or phenol red (virus plaquing

medium)

100 mL


Storage 2ºC-8ºC

Storage 2ºC-8ºC



91

Classical Media

McCoy’s 5A MediumDesigned for human lymphocyte culture.


12-168F BE12-168F McCoy‘s 5A Medium With L-Glutamine and 25 mM HEPES 500 mL

12-688F BE12-688F McCoy‘s 5A Medium With L-Glutamine 500 mL


Medium 199Medium 199 was originally formulated for chick embryo fibroblast culture. These four formulations require a CO2

enriched atmosphere.

Cat. No. Size With L-Glutamine With HEPES With HBSS With EBSS With 1.4g/L Na-

bicarbonate

With 2.2 g/L

Na-bicarbonate

12-117F 500 mL • • • •

12-117Q 1 L • • • •

12-118F 500 mL • • • •

12-119F 500 mL • • •

12-109F 500 mL • • •


12-109F BE12-109F Medium 199 With Hank‘s BSS, L-Glutamine, and 1.4 g/L NaHCO3 500 mL

12-117F BE12-117F Medium 199 With Earle‘s BSS, L-Glutamine, 25 mM HEPES, and 2.2 g/L NaHCO3 500 mL

12-117Q 12-117Q Medium 199 With Earle's BSS, L-Glutamine, 25 mM HEPES, and 2.2 g/L NaHCO3 1 L

12-118F BE12-118F Medium 199 With Hank's BSS, L-Glutamine, 25 mM HEPES, and 1.4 g/L NaHCO3 500 mL

12-119F BE12-119F Medium 199 With Earle‘s BSS, L-Glutamine, and 2.2 g/L NaHCO3 500 mL


Storage 2ºC-8ºC

Storage 2ºC-8ºC



92

Classical Media

PC-1™ Serum-free Medium Chemically Defined, General Purpose Serum-free Medium

PC-1™ Serum-free Medium – Chemically Defined is a low-protein medium intended for the culture of primary cells and anchorage-dependent cell lines. PC-1™ Medium contains a complete HEPES buffering system with known amounts of insulin, transferrin, fatty acids, and proprietary proteins assembled under strict quality control procedures. PC-1™ Medium is intended for a variety of research and industrial applications and is formulated using defined components for optimal cell growth, while maintaining the lowest possible protein content.

Cell Line Source Cell Type

HeLa Human Epithelial carcinoma, cervix

HTB-72 Human Malignant melanoma, epithelial-like

HTB-4 Human Bladder tumor

WI-38 Human Lung, diploidw

MRC-5 Human, male Embryonal lung, fibroblast-like

BHK-21 Syrian hamster Kidney, fibroblast-like

CHO-K1 Chinese hamster Ovary, epithelial-like

NRK Rat Normal kidney, epithelial/fibroblast-like

C6 Rat Glioma, primary

T9 Rat Glioma

ARL6T Rat Normal liver

3T3 Mouse Embryonic, fibroblast-like

STO Mouse Transformed fibroblast

VERO African green monkey Fibroblast

MDCK Dog Madin Darby canine kidney, epithelial-like

SIRC Rabbit Cornea

Partial List of Cell Cultures Cultivated with PC-1™ Serum-free Medium – Chemically Defined

Cultivation of primary and anchorage-dependent cells

Applications

Medium: 2°C to 8°C,

Supplement: -10°C to 20°C


93

Classical Media

Other Cell Types/Sources

Source Cell Type

Human

Neuroblastoma, foreskin fibroblast, bladder carcinoma, renal papillary collecting tubule (primary), colon epithe lium (primary), colon

carcinoma (primary)

Rat

Dermal fibroblast (primary), mammary carcinoma (primary), neonatal normal cardiac muscle (primary), thyroid epithelium (primary),

astrocytes (primary)

Baboon (Paprocynocephalus) spinal ganglia

Swine Testes cell

Bovine: Kidney


L-Glutamine 146

ProFreeze™ CD (2X) Freeze Medium – Chemically Defined 132


77232 77232 PC-1™ Serum-free Medium –

Chemically Defined

Complete medium system, including frozen supplement, without

L-Glutamine. For primary adherent cells.

2 × 500 mL


94

Classical Media

Partial lists of cell cultures cultivated with HL-1™ Serum-free Medium – Chemically Defined


U937 Human Macrophage

RaJi Human B lymphoblastic

MCF-7 (NIH) Human Breast carcinoma

MCF-7 (MCF) Human Breast carcinoma

NIH ZR-75 Human Breast carcinoma

COLO 302 HSR Human Colon carcinoma

J82 Human Bladder carcinoma

SW 1738 Human Bladder carcinoma

SW780 Human Bladder carcinoma

CCL 119 Human Lymphoid

CCL 213 Human Burkitt lymphoma

C91 / PL Human T lymphoma

Undesignated Human Astrocytoma

Undesignated Human Hepatoma

MOLT-3 Human Acute lymphoblastic leukemia

MOLT-4 Human Acute lymphoblastic leukemia

NAMALWA Human Burkitt lymphoma

THP-1 Human Monocytic leukemia

BB88 Mouse Erythroid (leukemia)

P815 Mouse Macrophage

Transformed and Established Cell Lines


WeHi3 Mouse Monocyte

JLS-V5 Mouse Spleen cell

70Z-3 Mouse Pre-B lymphoma

70Z / 3.12 Mouse B lymphoma

S49 and variants Mouse T lymphoma

RAW309F1.1 Mouse T lymphoma

WeHi7 Mouse T lymphoma

I-10 Mouse Leydig-tumor

EL4 Mouse T lymphoma

RL1 Mouse T lymphoma

BW5147.3 Mouse T lymphoma

LBRM-33 Mouse T lymphoma

Friend leukemia Mouse Leukemia

C57BL6 Mouse Embryo (C57 × DBA)

L5178Y Mouse Lymphoma (DBA / 2)

VERO African green

monkey

Fibroblast

MDCK Dog Madin Darby canine kidney

CHO K1 Hamster Chinese hamster ovary

(epithelial-like)

GCL2 Hamster | mouse B lymphoma × Normal B

HL-1™ Serum-free MediumChemically Defined Hybridoma Medium

HL-1™ Serum-free Medium – Chemically Defined is a culture medium containing less than 30 μg protein per mL. Components of HL-1™ Medium include HEPES buffer, insulin, transferrin, sodium selenite, ethanolamine, a variety of saturated and unsaturated fatty acids and proprietary stabilizing proteins. HL-1™ Medium contains no bovine serum albumin or other undefined protein mixtures. HL-1™ Medium supports the serum-free growth of various hybridomas, including those derived from P3X63Ag8.653 and Sp2/0-Ag14 myelomas, as well as other differentiated cells of lymphoid origin.

Serum-free growth of hybridomas and differentiated cells of lymphoid origin

HL-1™ Chemically Defined FBS Substitute (100X) is a chemically defined medium additive that can be used to replace serum or significantly reduce its concentration in a variety of basal media. It contains less than 30 μg protein per mL when diluted 1:100 in medium and it does not contain bovine serum albumin or other undefined protein ingredients.

HL-1™ FBS Substitute (100X) – Chemically Defined

Applications

Serum-free 2ºC-8ºCHL-1™ Fetal Bovine Serum 15°C to 30°C


95

Classical Media

Cat. No. NA Cat. No. EU Product Name Product Description Storage Conditions Size

77227 77227 HL-1™ Fetal Bovine Serum Substitute (100X) –

Chemically Defined

15°C to 30°C 10 mL

77201 BE77201 HL-1™ Serum-free Medium for Hybridoma and

Hematopoietic Cells – Chemically Defined

Without L-Glutamine 2°C to 8°C 2 × 500 mL

CE marked according to IVD Directive 98 / 79 / EC.


L-Glutamine 146

ProFreeze™ CD (2X) Freeze Medium – Chemically Defined 132


Hybridoma Source Fusion Partner

8A1 Human CLLC

undesignated Human WI-L2-729-HF2

undesignated Human LICR-LON-HMY2

HB44 Mouse Sp2 / 0-Ag14

HB45 Mouse Sp2 / 0-Ag14

HB56 Mouse NS-1

HB59 Mouse NS-1

HB60 Mouse P3X63Ag 8.653

53-7.313 Mouse NS-1

MI / 9.3.4HL-2 Mouse NS-1

MI / 70.15.1 Mouse NS-1

ARB Mouse Hybridoma

P3U Mouse P3X63Ag 8.653

BCS12 Mouse P3X63Ag 8.653

BCS 2002 Mouse P3X63Ag 8.653

undesignated Mouse NS-1

undesignated Mouse P3X63Ag 8.653

Hybridomas

Primary Cells

Cell Type

Human peripheral blood mononuclear cells

Mink lymphocytes

Human fetal adrenal

Human blood monocytes

Human peripheral blood T lymphocytes

Hybridoma Source Fusion Partner

TIB 175 Rat/mouse S194

TIB 104 Rat/mouse NS-1






RS Rat/mouse P3X63Ag 8.653

96

Classical Media

RPMI 1640 MediumRPMI is a general purpose media with a broad range of applications for mammalian cells, especially hematopoietic cells. RPMI with MOPS (04-525F) can be used with certain mycological assays.

Cat. No. Size Without L-GLutamine

Without Phenol Red

With HEPES With Penicillin Streptomycin

Without Na-Bicarbonate

With MOPS Buffer

With Ultra-Glutamine I

Without D-Glucose

12-702F 500 mL •

12-702Q 1 L •

BE12-702F/U1 500 mL •

12-167F 500 mL

12-167Q 1 L

BE12-115E 100 mL • •

12-115F/U1 500 mL • •

12-115F 500 mL • •

12-115Q 1 L • •

04-525F 500 mL • • •

BE04-558F 500 mL •

12-918F 500 mL •

09-774E 100 mL • • •

09-774F 500 mL • • •

BE12-752F 500 mL • •

Cat. No. NA Cat. No. EU Product Name Product Description Storage

Conditions

Size

BE12-702F/U1 RPMI 1640 With UltraGlutamine™ I 15°C to 30°C 500 mL

BE12-115F/U1 RPMI 1640 With UltraGlutamine™ I and 25 mM HEPES 15°C to 30°C 500 mL

04-525F 04-525F RPMI 1640 For certain mycological assays. With L-Glutamine and 165 mM MOPS, without sodium

bicarbonate

2°C to 8°C 500 mL

BE04-558F RPMI 1640 Without L-Glutamine, with 25mM HEPES 15°C to 30°C 500mL

09-774E 09-774E RPMI 1640 With L-Glutamine, 25 mM HEPES, 100 units/mL penicillin, and 50 μg/mL streptomycin 2°C to 8°C 100 mL

09-774F 09-774F RPMI 1640 With L-Glutamine, 25 mM HEPES, 100 units/mL penicillin, and 50 μg/mL streptomycin 2°C to 8°C 500 mL

12-115F BE12-115F RPMI 1640 With L-Glutamine and 25mM HEPES 2°C to 8°C 500 mL

12-115Q 12-115Q RPMI 1640 With L-Glutamine and 25mM HEPES 2°C to 8°C 1 L

12-167F BE12-167F RPMI 1640 Without L-Glutamine 15°C to 30°C 500 mL

12-167Q 12-167Q RPMI 1640 Without L-Glutamine 15°C to 30°C 1 L

12-702F BE12-702F RPMI 1640 With L-Glutamine 2°C to 8°C 500 mL

12-702Q 12-702Q RPMI 1640 With L-Glutamine 2°C to 8°C 1 L

12-918F BE12-918F RPMI 1640 Without L-Glutamine or phenol red 15°C to 30°C 500 mL

15-702D 15-702D RPMI 1640 With L-Glutamine 2°C to 8°C 10 L

BE12-752F BE12-752F RPMI 1640 With L-Glutamine, without D-glucose 2°C to 8°C 500 mL



97

Classical Media

Cat. No. NA Cat. No. EU Product Name Product Description Storage Conditions Size

12-923E 12-923E NCTC-109 Medium With Earle‘s BSS and L-Glutamine, hybridoma screened 2°C to 8°C 100 mL


BE02-019F BE02-019F William‘s Medium E Without L-Glutamine or phenol red 500 mL

BE12-761F William‘s Medium E Without L-Glutamine 500 mL

NCTC-109 MediumA complex formula used to supplement hybridoma medium.

William’s Medium E

Richter’s CM MediumA complex formula used to supplement hybridoma medium


04-648F 04-648F Richter’s CM Medium With L-Glutamine 500 mL




Storage 2ºC-8ºC

Storage 2ºC-8ºC

Storage 2ºC-8ºC




98


12-743F BE12-743F UltraMEM™ Reduced Serum Medium – Chemically Defined Low protein medium with ITES and L-Glutamine 500 mL

UltraMEM™ Reduced Serum MediaChemically Defined, General Purpose Serum-free Media

UltraMEM™ Reduced Serum Media – Chemically Defined is designed to support growth and maintenance of several anchorage-dependent cell types under reduced serum concentrations (see table). When supplemented with 2–4% serum, UltraMEM™ Media growth performance is comparable and, in some instances, exceeds that of standard media supplemented with 10% fetal bovine serum. Weaning is not necessary for most applications. Further reduction in serum concentration (<2%) can be achieved over several passages.

Growth and maintenance of anchorage-dependent cell types

Applications

Reduced Serum Medium – Chemically Defined

Cell Type Recommended % Serum

Diploid

CEF, chicken fibroblast 2% FBS

WI-38, human fibroblast 3% FBS

RHMK, monkey kidney 2–4% FBS

MRC-5, human fibroblast 3% FBS

RK, rabbit kidney 3% FBS

BGMK, monkey kidney 2–3% FBS

HEL, human fibroblast 2–3% FBS

CMK, monkey kidney 3–4% FBS

Heteroploid

VERO, monkey kidney 3% FBS

CRFK, cat kidney 2–3% FBS

L929, mouse fibroblast 4% FBS

MDCK, dog kidney 2–3% FBS

McCOY, mouse fibroblast 4% FBS

3T3, mouse fibroblast 3–4% FBS

CHO-K1, hamster ovary 3% FBS

PK-15, pig kidney 3–4% FBS

MK2, monkey kidney 3–4% FBS


Classical Media

Partial List of Cell Cultures Cultivated with UltraMEM™

Storage 2ºC-8ºC

Features of the standard productIn addition, confluent cultures can be maintained with minimal amounts of serum (≤1%) or no serum at all. Growth performance in UltraMEM™ Media can be further increased by the addition of insulin, transferrin, and selenium to the basal medium UltraMEM™ Reduced Serum Medium, offered as a complete low protein medium supplemented with ITES. Recombinant human insulin and transferrin are the only protein components of the complete formulation and are present at a total concentration of 20 μg/mL.

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p.100

Sartopore® 2 XL Filter Family

p.102

Sartogard PreFilter Familyy

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Virosart® p.104

UVivatec® p.105

Flexel® for Mixing1 p.106

FlexAct® MP p.107

Which Bioreactor Fits your Needs?

p.108

Cell culture media are produced in large volumes and aseptically transferred into the bioreactor. Key process steps involved in media preparation are the mixing of the powder media with water for injection (WFI), the sterilizing-grade and mycoplasmaretentive filtration and the media storage before use. Continuous cost pressure, increased titers and reduced production volumes have led to the adoption of single-use technologies – both for bioreactors and media preparation.

Sartorius has therefore developed the fully automated, easy-to-use FlexAct® MP system that helps to reduce manpower even further. As your leading partner for upstream processing, Sartorius you with integrated solutions that comprise cell culture media, bags, sterilizing grade filters, mycoplasma retentive filters and virus risk mitigation tools.

Mixing Bags

Sterilizing Grade Filters

Mycoplasma Retentive Filters

Virus Retention

Filters

UV Virus Inactivation

Storage Bags

Automated Media Preparation

Cell Culture Media

99

100

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Single-use Media Preparation

Today, single-use systems for media preparation are available up to 3.000 L volume. While users initially converted individual production components to single-use, the industry is now moving to completely integrated systems for media preparation. Sartorius FlexAct® MP media preparation platform is a highly automated, fully closed system: It starts with powder dissolution in a single-use mixing system followed by sterile filtration and the transfer of the ready-to-use media into a storage bag.

Virus Risk Mitigation in Media Preparation

Virus risk mitigation in cell culture media preparation is a hot topic right now. Almost all product contaminations during the past several years were caused by small, nonenveloped viruses derived from raw materials. As a result, virus removal technologies that are effective for small, non-enveloped viruses gain importance in upstream processing. Technologies such as high Temp short time treatment HTST (Hight Temperature Short Time), UV-C inactivation as well as virus filtration have been employed for virus risk mitigation in cell culture media preparation. UV-C inactivation is also applicable for virus inactivation of serum and serum-containing media. Virosart filters have been successfully applied at low flow rates for chemically defined media filtration in continuous perfusion processes.– Broad range of virus filters for different media filtration applications.– Safe virus inactivation in serum and serum containing media by UV-C inactivation.– Orthogonal and robust technologies prevent contaminations.

Powder Media Mixing Filtration Storage› › ›

Solutions for Media Preparation

In order to avoid errors, the entire media preparation process is supervised by a multifunctional central control unit. It monitors and records all relevant process data, such as operating pressure, pH, pump speed and fluid level. Sartorius filters and single-use bags are supported by an industry-leading documentation package, including validation and extractables guides. Our validation experts support the customers during qualification of single-use systems for biopharmaceutical production.

– Complete range of single-use bags for media preparation, mixing and storage from 50 L up to 3.000 L volume for scale-up from R & D to production with proven performance.

– Magnetic mixing technology with universal mobile drive unit

– Fully integrated media preparation platform FlexAct® MP.

– Comprehensive validation support for extractables | leachables by dedicated CONFIDENCE validation team.

Media Filtration Solutions

The choice of an appropriate filtration solution for media preparation highly depends on the composition of the cell culture medium. Often 0.2 μm-rated sterilizing-grade filters are used for media preparation. However, reports of mycoplasma contamination of cell culture processes have put 0.1 μm-rated mycoplasma-retentive filters into the spotlight, again. Typical sources for mycoplasma contamination are animal-derived materials such as trypsin or serum, plant-derived culture media supplements such as soy-based supplements as well as contaminations caused by the operators themselves. Producers of cell culture media have to assess their potential risk for mycoplasma contamination in order to decide which filtration method to use. Sartorius media filtration solutions range from prefilters to sterilizing grade and mycoplasma-retentive filters – tailored to the specific filtration needs of the various types of cell culture media.

101

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Serum, soy oryeast hydrolysatesupplementedcell culture media

Chemicallydefinedcell culturemedia

Serum orhydrolysatesupplementedmedia

Complex additives withbroad molecular weightdistribution and potentialkolloid and particle load

Might contain rec. proteinadditives such as insulin,transferin or othergrowth factors

Potential mycoplasmacontamination risk

Sartoguard GFGlas fibre fleece0.2 μm | 0.2 μm

Sartopore® 2XLG0.8 μm | 0.2 μm

Sartoguard NFNanofleece0.2 μm | 0.2 μm

Sartopore® 2XLG0.8 μm | 0.2 μm

Sartoguard PES0.2 μm | 0.1 μm

Sartopore® 2XLM0.2 μm | 0.1 μm

Media Type

Characteristics

Prefilter Options

Final Filter Options

Which Filter Solution Best Fits Your Needs?Today’s industrial cell culture media formulations can be very different, from serum and hydrolyzate supplemented to chemically defined media. Based on our long standing experience in media filtration, we can recommend the best filter solution for your needs. Our international team of application specialists can help you optimize your specific filtration process – just contact us today.

Sartorius offers:

– A broad portfolio of final membrane filters for various media types, including chemically defined or protein, serum or hydrolysate supplemented with highest total throughput and economic filtration system design.

– Secure solutions for mycoplasma risk mitigation combining high retention efficiency with optimal throughput for economic processing.

– A refilter portfolio with optimal protection of final filters to further reduce filtration costs– All media filtration solutions are available for single use processing or media production in stainless steel equipment.

102

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Sartopore® 2 XL Filter FamilyBest Filtration Solutions for Cell Culture Media

The Sartopore® 2 XL filter family is the best choice for sterilizing-grade filtration and mycoplasma-retentive filtration of cell culture media.

The unique range of PES double-layer membrane combinations perfectly serves the different cell culture media requirements. Sartopore® 2 XLM filters use double-layer PES membranes with pore size combination of 0.2 | 0.1 μm for reliable mycoplasma retention with an LRV of 7/cm2 filtration area.

They have additionally been validated for sterilizing grade filtration according to ASTM F 838-05. Sartopore. XL ensures unmatched filtration performance along with the highest microbial safety.

Unique combination of different pre- and final filter membranes

k Ensures highly economic filter sizing and minimum filtration costs for all kind of media

Low unspecific protein binding k Excellent and reproducible cell growth

Reliable retention of mycoplasma by Sartopore 2XLM 0.1μm k Prevents mycoplasma contamination of your cell culture process

Available in a broad range of sizes and formats to provide linear scale-up from development to commercial-scale production

k Identical filtration performance at all scale-up levels of your media preparation process

Applications

– Sterilizing-grade filtration and mycoplasma-retentive filtration of cell culture media

– Sartopore 2 XLG (08 | 0.2 μm) designed for complex. serum or hydrolyzate supplemented media and for chemically defined media

– Sartopore. 2 XLM (0.2 | 0.1 μm) designed for mycoplasma retention (0.1 μm)

103

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Sartoguard Prefilter FamilyOptimal Protection for Sartopore® 2 XL Filter Elements

The Sartoguard family consisting of PES, GF and NF versions are all designed to extend the lifetime of Sartopore® 2 XL final filters by a factor of 3 to 4 – for cost-effective cell culture media prefiltration. Sartoguard filters provide the most cost-effective solutions to ensure your final filter achieves the highest throughput. Sartoguard PES contains a PES double layer, available in both 0.1 and 0.2 μm nominal pore size configurations. They are designed for cost-effective prefiltration of chemically defined media.Sartoguard NF is a gamma-irradiatable prefilter which contains a PES double layer covered with a modern PES naNofleece material. Sartoguard GF contains a PES double layer covered with a glass fiber fleece. It effectively protects the filter from clogging, especially when you need to filter serum and hydrolysate supplemented cell culture media.

Heterogeneous PES membrane double layer with or without fleece material− provides optimal prefiltration for all types of

cell culture media− protects subsequent sterile filters and extend

filtration capacity

Sartoguard prefilter family k Protects subsequent sterile filters and extend filtration capacity

Sartoguard PES k For prefiltration of chemically defined media

Sartoguard NF k For prefiltration of serum-free media with higher tendency of clogging final filter

Staroguard GF k For prefiltration of complex media containing serum of hydrolysates

– Prefiltration of cell culture media– Protection of Sartopore XL final filters

Applications

104

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Virosart®

Optimal Protection for Sartopore® 2 XL Filter Elements

Virus-retentive filtration is a highly efficient method for viral risk mitigation of cell culture media as these filters target both Non-enveloped viruses, such as MVM and PPV, and large enveloped viruses, such as MLV. Sartorius’ robust virus removal technology has been proven throughout the industry as providing the highest safety for your cell culture without impacting their performance.

You can choose from three virus-retentive membranes to suit your specific media composition, e.g., hydrolysate and protein content.

Unlike most other virus retentive filters, all Sartorius virus-retentive filters reliably and robustly provide > 4 LRV for small non-enveloped viruses over the entire flow decay profile.

Robust performance over a wide range of flow rates and process conditions

k The highest possible safety

Sterile and gamma-irradiated virus removal filter set-ups (e.g. Virosart® HF)

k – Ready-to-use– No need for preflushing

High-performance PES membranes k – Capacity from 1,000 to 10,000 L/m2 achieved for in-line virus-retentive filtration in perfusion cultures

– No impact on cell culture performance

Integrity testing with Sartocheck® 4 Plus Filter tester (based on diffusion)

k Easy, safe, fast, cost-effective

– Viral risk mitigation of chemically defined media– Viral risk mitigation of perfusion media feeds– Sterile media– Low protein and hydrolysate content cell culture media

Applications

105

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UVivatec®

Virus Inactivation Based on UV-C Irradiation

Virus inactivation by UV-C irradiation is a highly efficient technology that specifically targets small, non-enveloped viruses (e.g., PPV, MVM) at low doses.

Difficult-to-filter and heat-instable media components like serum can be safely inactivated by UV-C (254 nm) without impacting cell growth or cell viability.

Even highly turbid media or serum can be easily exposed to the UV source without any risk of blocking the flow path. Small, non-enveloped viruses like MVM show good clearance of six LRV already at low doses of around 100–150 J/m2 while maintaining cell growth performance.

– Serum virus inactivation– Virus inactivation of serumcontaining media– Virus inactivation of heat sensitive components

Inactivation based on UV-C (254 nm) irradiation

k Especially effective for small, non-enveloped viruses (e.g., MVM)

Novel helical module with optimized hydrodynamic design

k – Uniform irradiation through efficient radial mixing based on Dean vortices– Narrow residence time distribution– Effective inactivation at low doses

Single-use module concept k – Quick and easy to install and maintain– Easy to scale

Applications

106

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Flexel® for Mixing1Single-Use Media Mixing Solution

Flexel® for Magnetic Mixer® 1 is a compact and non-invasive single-use mixing system for manual media dissolution and mixing.The system is based on magnetic coupling, e.g., no shaft-through or dynamic seal is used, providing a completely closed mixing assembly.Agitation rates of up to 300 rpm can be reached, allowing highly efficient mixing from 50 – 3,000 L scale.

Flexel® for Magnetic Mixer. 1 with its strong magnetic coupling is the ideal solution for:− Powerful dissolution of powder media with high

torque and high rotation speed− Effective mixing of highly viscous solutions,

such as media and hydrolysate mixtures− Large volumes (up to 3,000 L)

– Media | feed preparation– Buffer preparation

Cubical shape with front access system and large 8” diameter top port k Bag installation, filling, powder transfer and draining steps easy to perform

Baffle-like effect with strong top to bottom flow pattern thanks to the centered impeller in combination with the cubical design

k High performance proven from 50-3,000 L

Pre-assembled and presterilized single-use sensors for pH, conductivity and temperature

k In-line monitoring and control of critical process parameters

Palletank® for mixing with heat exchange jacket and integrated weighing functions

k Product temperature and volume control

Dust-free powder addition via closed addition bag k Protection of operators, meeting current health and safety regulations

1 This product uses Pall patented Magnetic Mixer technology.All information on patents can be found at Pall.com/patents.

Applications

107

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FlexAct® MPConfigurable Single-Use Solution for Media Preparation

The FlexAct® MP is a standardized configurable system for convenient media preparation in biopharmaceutical processes. The FlexAct® central operation module enables you to control and monitor all relevant process parameters during media preparation.Integrated weighing capabilities permit precise media dissolution using our Magnetic Mixer®1 technology. A fully automated, temperature-controlled pH adjustment is performed via a jacketed Palletank® for mixing and a temperature control unit (TCU).During fluid transfer and sterile filtration, pressure and flow are monitored and controlled to ensure safe operation.

Multi-functional central operating module k Operator-friendly

Tailored bag configuration k Flexible media supply

50-3,000 L working volume k Fully scalable

Quick system set-up k Increased efficiency

Integrated disposable sensors (pH, conductivity, temperature) k Reliable monitoring of important parameters

Bi-directional operation k Highly flexible

1 MagMixer® is a trademark of Pall Corporation and this product uses Pall patented MagMixer® technology.All information on patents can be found at Pall.com/patents.

– Automatic, contained media preparationApplications

108

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Which Bioreactor Fits your Needs?

Available for Cell Culture (CC) or Microbial (MO)

Culture

Screening ofMedia, Clonesor ExpressionConstructs

Small ScaleProteinSupply

ProcessDevelopmentOptimization,Characteri-

sation

Seed Expansion Production Product Product

Vessel TypeNumber

of ParallelVessels

Max. VesselWorking VolumeSingle-Use Glass Stainless Steel

CC ☐ (☐)ambr® 15 ambr® 15

h24 | 48 10 – 15 mL

MO

CC ☐ (☐) ☐ ambr® 250 ambr® 250

h12 | 12 100 – 250 mL

MO ☐ (☐) ☐ h

CC ☐ ☐ (☐) ☐CERTOMAT® CTplus CERTOMAT® CTplus

h6 | 98 12.5 mL – 2.5 L

MO

CC (☐) ☐ (☐) ☐BIOSTAT® A BIOSTAT® A

h h1 1 – 5 L

MO (☐) ☐ (☐) ☐ h

CC ☐ ☐ ☐ ☐BIOSTAT® B BIOSTAT® B

h h2 1 – 10 L

MO ☐ ☐ ☐ ☐ h

CC ☐ ☐ ☐ ☐BIOSTAT® B-DCU II BIOSTAT® B-DCU II

h h6 0.5 – 10 L

MO ☐ ☐ ☐ ☐ h

CC ☐ ☐ ☐ (☐)BIOSTAT® Cplus BIOSTAT® Cplus

h1 5 – 30 L

MO (☐) ☐ ☐ ☐ (☐) h

CC ☐ ☐ ☐ ☐BIOSTAT® D-DCU BIOSTAT® D-DCU

h2 10 – 200 L

MO ☐ ☐ ☐ ☐ h

CC ☐ ☐ Customized Stainless Steel Fermenters

Customized Stainless Steel Fermenters

hmultiple 200 – 2,000 L

MO ☐ ☐ h

CC ☐ ☐ (☐)BIOSTAT® RM BIOSTAT® RM

h2 0.5 – 100 L

MO (☐) (☐) (☐) h*

CC ☐ ☐ ☐ ☐BIOSTAT STR® BIOSTAT STR®

✓2 50 – 2,000 L

MO (☐) (☐) (☐) (☐) ✓*

☐ Typical Applications(☐) Selected Applications

ambr® 15 ambr® 250 CERTOMAT® CTplus Biostat® A Biostat® B

109

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Available for Cell Culture (CC) or Microbial (MO)

Culture

Screening ofMedia, Clonesor ExpressionConstructs

Small ScaleProteinSupply

ProcessDevelopmentOptimization,Characteri-

sation

Seed Expansion Production Product Product

Vessel TypeNumber

of ParallelVessels

Max. VesselWorking VolumeSingle-Use Glass Stainless Steel

CC ☐ (☐)ambr® 15 ambr® 15

h24 | 48 10 – 15 mL

MO

CC ☐ (☐) ☐ ambr® 250 ambr® 250

h12 | 12 100 – 250 mL

MO ☐ (☐) ☐ h

CC ☐ ☐ (☐) ☐CERTOMAT® CTplus CERTOMAT® CTplus

h6 | 98 12.5 mL – 2.5 L

MO

CC (☐) ☐ (☐) ☐BIOSTAT® A BIOSTAT® A

h h1 1 – 5 L

MO (☐) ☐ (☐) ☐ h

CC ☐ ☐ ☐ ☐BIOSTAT® B BIOSTAT® B

h h2 1 – 10 L

MO ☐ ☐ ☐ ☐ h

CC ☐ ☐ ☐ ☐BIOSTAT® B-DCU II BIOSTAT® B-DCU II

h h6 0.5 – 10 L

MO ☐ ☐ ☐ ☐ h

CC ☐ ☐ ☐ (☐)BIOSTAT® Cplus BIOSTAT® Cplus

h1 5 – 30 L

MO (☐) ☐ ☐ ☐ (☐) h

CC ☐ ☐ ☐ ☐BIOSTAT® D-DCU BIOSTAT® D-DCU

h2 10 – 200 L

MO ☐ ☐ ☐ ☐ h

CC ☐ ☐ Customized Stainless Steel Fermenters

Customized Stainless Steel Fermenters

hmultiple 200 – 2,000 L

MO ☐ ☐ h

CC ☐ ☐ (☐)BIOSTAT® RM BIOSTAT® RM

h2 0.5 – 100 L

MO (☐) (☐) (☐) h*

CC ☐ ☐ ☐ ☐BIOSTAT STR® BIOSTAT STR®

✓2 50 – 2,000 L

MO (☐) (☐) (☐) (☐) ✓*

✓ Available✓* Available for low cell density applications

BIOSTAT® B-DCU II BIOSTAT® Cplus BIOSTAT® D-DCUCustomized Stainless Steel

FermentersBIOSTAT® RM BIOSTAT STR®

110

AO Animal or human Origin

BHK Baby Hamster Kidney

BME Basal Medium Eagle

CD Chemically Defined

cGMP Current Good Manufacturing Practices

CHO Chinese Hamster Ovary

CoA | C of A Certificate of Analysis

CRO Contract Research Organization

CTM Clinical Trial Management

DF Dia Filtration

DMEM Dulbecco's Modified Eagle Medium

DOE Design of Experiment

DPBS Dulbecco's Phosphate Buffered Saline

DSP Down Stream Processing

EMA European Medicine Agency

ESC Embryonic Stem Cell

F&F Fill and Finish

FBS Fetal Bovine Serum

FDA Food and Drug Agency

FGM Fibroblast Growth Medium

FMU For Further Manufacturing Use

FTM Fluid Thioglycollate Medium

GMEM Glasgow Minimum Essential Medium

HEK Human Embryonic Kidney

hPSC Human Pluripotent Stem Cell

Glossary

Comprehensive Glossary for All Abbreviations

111

Glossary

IMDM Iscove's Modified Dulbecco's Medium

iPSC Induced Pluripotent Stem Cell

KGM Keratinocyte Growth Medium

LAK Lymphocyte Activated Killer

MDCK Madin Carby Canine Kidney

MEM Minimal Essential Medium

MSCBM Mesenchymal Stem Cell Basal Medium

MSCGM Mesenchymal Stem Cell Growth Medium

NAO Non-Animal and non-human Origin

NK Natural Killer

PBL Peripheral Blood Lymphocyte

PBS Phosphate Buffered Saline

PDT Population Doubling Time

PF* Protein Free

R&D Research and Development

RUO Research Use Only

Sf Spodoptera frugiperda

SSB Sartorius Stedim Biotech

TIL Tumor Infiltrating Lymphocyte

TSB Trypticase Soy Broth

UF Ultra Filtration

USP Up Stream Processing

USP | EP US Pharmacopeia | European Pharmacopeia

WFI Water for Injection

*human recombinant insulin is NOT considered a protein, both because it is fully characterized, and because of its size (<5kDa)

112

Complete Product List

Cat. No NA Cat. No EU Product Description Size CATEGORY CATALOGUE

BE12-730P10 BE12-730P10 STD Insect-XPRESS w/ L-Gln, 10 L bag 10 L 1) Multi Purpose Media

BP12-730Q BEBP12-730Q Insect-XPRESS w/ L-Gln 1 L 1 L 1) Multi Purpose Media

BP12-730F BEBP12-730F Insect-XPRESS w/ L-Gln, 500 mL 500 mL 1) Multi Purpose Media

BP12-725F BEBP12-725F UltraCULTURE serum-free w/o L-Gln 500 mL 500 mL 1) Multi Purpose Media

04-501Q 04-501Q Grace’s Insect Medium (TNM-FH) w/FBS 1 L 1 L 1) Multi Purpose Media

04-649F 04-649F Grace’s Insect Medium w/o FBS, 500 mL 500 mL 1) Multi Purpose Media

04-457F 04-457F Grace’s Insect w/o FBS, Gent. LAH, 500 mL 500 mL 1) Multi Purpose Media

04-351Q 04-351Q Schneider’s Drosophila Medium, Mod., 1 L 1 L 1) Multi Purpose Media

N/A BE02-011F TC100 Insect Medium 500 mL 500 mL 1) Multi Purpose Media

02-044Q BE02-044Q STD PowerFeed A, 1 L bottle 1 L 2) Proteins

02-059F BE02-059F STD FeCitrate100x-PowerCHO™-2KIT, 500 mL bt 500 mL 2) Proteins

02-059Q BE02-059Q STD FeCitrate100x-PowerCHO™-2KIT, 1 L bt 1 L 2) Proteins

12-771P10 BE12-771P10 STD PowerCHO™-2 CD, w/o L-Gln w/o PR, 10 L 10 L 2) Proteins

12-771P20 BE12-771P20 STD PowerCHO™-2 CD, w/o L-Gln w/o PR, 20 L 20 L 2) Proteins

15-044D BE15-044D STD PowerFeed A Powder kit 10 L 10 L 2) Proteins

15-044F BE15-044F STD PowerFeed A Powder kit 50 L 50 L 2) Proteins

15-044J BE15-044J STD PowerFeed A Powder kit 100 L 100 L 2) Proteins

15-044Q BE15-044Q STD PowerFeed A Powder kit 200 L 200 L 2) Proteins

15-766D BE15-766D STD ProCHO5 Powder kit w/o PR 10 L 10 L 2) Proteins

15-766F BE15-766F STD ProCHO5 Powder kit w/o PR 50 L 50 L 2) Proteins

15-766L BE15-766L STD ProCHO5 Powder kit w/o PR 500 L 500 L 2) Proteins

15-771ND BE15-771ND STD PowerCHO™2 Powder kit 10 L 10 L 2) Proteins

15-771NF BE15-771NF STD PowerCHO™2 Powder kit 50 L 50 L 2) Proteins

15-771NJ BE15-771NJ STD PowerCHO™2 Powder kit 100 L 100 L 2) Proteins

12-927Q 12-927Q STD PowerCHO™ Advance™ 1 L bt 1 L 2) Proteins

BE02-043E BE02-043E STD FeCitrate1000x PowerCHO™-GS kit, 100 mL 100 mL 2) Proteins

BEBP02-052Q BEBP02-052Q STD Power Feed A with Lipids – 1 L bottle 1 L 2) Proteins

BE02-056Q BE02-056Q STD CHO Xtreme Feed™ CD – 1 L bottle 1 L 2) Proteins

BE12-766P10 BE12-766P10 STD ProCHO5w/Pluronicw/oL-Gln, HT, 10 L 10 L 2) Proteins

BE12-766P20 BE12-766P20 STD ProCHO5w/Pluronicw/o L-Gln, HT, 20 L 20 L 2) Proteins

BE15-771D BE15-771D STD PowerCHO™2 Powder kit 10 L 10 L 2) Proteins

BE15-771F BE15-771F STD PowerCHO™2 Powder kit 50 L 50 L 2) Proteins

BE15-771J BE15-771J STD PowerCHO™2 Powder kit 100 L 100 L 2) Proteins

BE15-776D BE15-776D STD PowerCHO™ GS Powder kit 10 L 10 L 2) Proteins

N/A BE15-770F STD PowerCHO™1 Powder kit 50 L 50 L 2) Proteins

N/A BE15-771L STD PowerCHO™2 Powder kit 500 L 500 L 2) Proteins

N/A BE15-776F STD PowerCHO™ GS Powder kit 50 L 50 L 2) Proteins

BP12-770Q BP12-770Q PowerCHO™-1 serum-free medium 1 L 1 L 2) Proteins

BEBP17-855E BEBP17-855E ProHT Supplement 100 × 100 mL 100 mL 2) Proteins

BEBP12-776Q BEBP12-776Q PowerCHO™ - GS, 1L bottle 1 L 2) Proteins

N/A BEBP02-042Q PowerCHO™-2 CD, w/o glucose, 1 L 1 L 2) Proteins

BP12-771Q BEBP12-771Q PowerCHO™-2 CD, w/o L-Gln w/o PR or HT, 1 L 1 L 2) Proteins

113



BP12-772Q BEBP12-772Q PowerCHO™-3 CD, w/o L-Gln w/o PR or HT, 1 L 1 L 2) Proteins

BP04-919Q BP04-919Q ProCHO 4 w/o Phenol Red, 1 L 1 L 2) Proteins

BP12-029Q BEBP12-029Q ProCHO4 w/o L-Gln, HT 1 L 1 L 2) Proteins

N/A BEBP02-041Q ProCHO5 w/o glucose, 1 L 1 L 2) Proteins

BP12-766Q BEBP12-766Q ProCHO5 w/Pluronic w/o L-Gln or HT, 1 L 1 L 2) Proteins

BEBP02-016Q BEBP02-016Q PROCHO-AT w/L-Gln 1L, w/o HT 1 L 2) Proteins

BEBP02-029Q BEBP02-029Q ProDoma 1 serum-free medium, 1 L 1 L 2) Proteins

BEBP02-032Q BEBP02-032Q ProDoma 3 woL-gln,PR w 0.1% Pluro F68 1L 1 L 2) Proteins

BP12-724Q BP12-724Q UltraCHO w/L-Gln 1 L 1 L 2) Proteins

N/A BEBP12-723F UltraDOMA serum-free w/o L-Gln 500 mL 500 mL 2) Proteins

BP12-723B BP12-723B UltraDOMA SF hybridoma medium, 500 mL 500 mL 2) Proteins

BP12-727F BP12-727F UltraDOMA-PF Hybridoma Medium, 500 mL 500 mL 2) Proteins

BESP904 BESP904 Lonza medium base 1 20,18 g 2) Proteins

BESP905 BESP905 Feed Base K 10,23 g 2) Proteins

BESP906 BESP906 Feed Base L 28,7 g 2) Proteins

BESP907 BESP907 Feed Base M 2,36 g 2) Proteins

BESP908 BESP908 Feed Base N 0,18 g 2) Proteins

BESP799 BESP799 Feed base F powder 1,75 g 2) Proteins

BESP862 BESP862 Feed base E powder 1,72 g 2) Proteins

BESP808 BESP808 CHO Vitamin Mix 0,18 g 2) Proteins

BESP794 BESP794 Feed Base A 43,06 g 2) Proteins

BESP795 BESP795 Feed Base B 112,2 g 2) Proteins

BESP734 BESP734 NMB1 22,04 g 2) Proteins

BESP735 BESP735 NMB2 18,34 g 2) Proteins

BESP1023 BESP1023 Feed Base R 114,52 g 2) Proteins

BESP876 BESP876 Feed Base 2 6,47 g 2) Proteins

BESP887 BESP887 MEM Vitamin Powder 9,31 g 2) Proteins

BESP899 BESP899 Feed Base G 42,63 g 2) Proteins

BESP900 BESP900 Feed Base H 69,04 g 2) Proteins

BESP901 BESP901 Feed Base J 133,87 g 2) Proteins

BESP1021 BESP1021 LMdB2 19,97 g 2) Proteins

BESP1022 BESP1022 Feed Base P 72,17 g 2) Proteins

BESP797 BESP797 Feed Base D 51,88 g 2) Proteins

BESP531E BESP531E SPE 100 mL 2) Proteins

BESP531F BESP531F SPE 500 mL 2) Proteins

BESP531Q BESP531Q SPE 1000 mL 2) Proteins

BESP909E BESP909E LTEM ONE 100 mL 2) Proteins

BESP909Q BESP909Q LTEM ONE 1000 mL 2) Proteins

BESP910E BESP910E LTEM TWO 100 mL 2) Proteins

BESP910Q BESP910Q LTEM TWO 1000 mL 2) Proteins

BESP969E50 BESP969E50 SP6 50 mL 2) Proteins

BESP969F BESP969F SP6 500 mL 2) Proteins


114





BESP971E BESP971E SP8 100 mL 2) Proteins



BESP807E BESP807E CHO Trace Element Stock Solution 100 mL 2) Proteins

BESP807Q BESP807Q CHO Trace Element Stock Solution 500 mL 2) Proteins

12-924Q N/A ProMDCK (2D) 1 L 3)Vaccines

12-925Q N/A ProMDCK (3D) 1 L 3)Vaccines

BE02-039Q BE02-039Q GLS PERMEXCIS® virus production med, 1 L 1 L 3)Vaccines

BEBP02-028Q BEBP02-028Q ProPer-1 wo L-gln,PR w 0.1% Pluro F68 1 L 1 L 3)Vaccines

BP12-764Q BEBP12-764Q Pro293A-CDM, for adherent cells, 1 L 1 L 3)Vaccines

N/A BEBP02-025Q Pro293s-CDM, 1 L bottle 1 L 3)Vaccines

BP12-765Q BP12-765Q Pro293S-CDM, for suspension cells, 1 L 1 L 3)Vaccines

BEBP02-030Q BEBP02-030Q ProVero-1 NAO Medium for Vero Cells, 1 L 1 L 3)Vaccines

BP12-749Q BEBP12-749Q UltraMDCK serum-free 1 L 1 L 3)Vaccines

08-879H 08-879H STD X-Vivo 15 w/o gentamicin and pr, 5L 5 L 4) Regenerative Medicine

BEBP04-743Q BEBP04-743Q X-VIVO 10 w/o Gentamycin w/o PR 1L 1 L 4) Regenerative Medicine

BP04-380Q BEBP04-380Q X-Vivo 10 w/ Gentamycin and PR, 1L 1 L 4) Regenerative Medicine

BP04-418Q BEBP04-418Q X-Vivo 15 w/ Gentamycin and PR, 1 L 1 L 4) Regenerative Medicine

N/A BEBP04-418F X-Vivo 15 w/ Gentamycin and PR, 500 mL 500 mL 4) Regenerative Medicine

BP04-744Q BEBP04-744Q X-VIVO 15 w/o Gent or Phenol Red, 1 L 1 L 4) Regenerative Medicine

BP04-448Q BEBP04-448Q X-Vivo 20 w/ Gentamycin and PR, 1 L 1 L 4) Regenerative Medicine

BEBP02-055Q BEBP02-055Q X-VIVO10(04-743) contains rec transf -1 L 1 L 4) Regenerative Medicine

BEBP02-053Q BEBP02-053Q X-Vivo15(04-418) contains rec transf, 1 L 1 L 4) Regenerative Medicine

BEBP02-054Q BEBP02-054Q X-Vivo15(04-744) contains rec transf, 1 L 1 L 4) Regenerative Medicine

CC4455 CC4455 BulletKit KGM-CD Kit 4) Regenerative Medicine

CC-4456 CC-4456 BulletKit KGM-CD EA CC3255+CC4455 Kit 4) Regenerative Medicine

199019 199019 FBM-CD 4) Regenerative Medicine

199020 199020 FGM-CD SinglQuots kit Kit 4) Regenerative Medicine

190620 190620 MSCBM-CD™ 500 mL 500 mL 4) Regenerative Medicine

00199041 00199041 FBM-CD Bullet Kit Kit 4) Regenerative Medicine

190632 190632 MSCGM-CD bulletkit Kit 4) Regenerative Medicine

192125 192125 MSCGM-CD SingleQuots Kit Kit 4) Regenerative Medicine

CC-4126 CC-4126 FGM-2 SingleQuotKit Suppl&Growth Factors Kit 4) Regenerative Medicine

CC-3131 CC-3131 FBM Fibroblast Basal Medium, 500 mL 500ml 4) Regenerative Medicine

CC-3132 CC-3132 FGM-2 BulletKit (CC-3131 & CC-4126) Kit 4) Regenerative Medicine

04-1193E N/A L7™ hPSC Passaging Solution 100 mL 4) Regenerative Medicine

00215550 N/A L7™ hPSC Cryosolution 50 mL 4) Regenerative Medicine

00215549 N/A L7™ hPSC Matrix 1 mg 4) Regenerative Medicine

00215506 N/A L7™ hPSC Media BulletKit™ 500 mL Basal medium +

5 mL Supplement

4) Regenerative Medicine

115



04-1191F N/A L7™ hPSC Basal Medium 500 mL 4) Regenerative Medicine

04-1192J N/A L7™ hPSC Supplement 5ml 4) Regenerative Medicine

N/A BE02-038P200 Bulk Packaged sterile filtered WFI-200 L 200 L 5) Buffers

N/A BE15-512F STD DPBS Powder w/o Ca, Mg 50 L 50 L 5) Buffers

N/A BE15-512K STD DPBS Powder w/o Ca, Mg 1000 L 1000 L 5) Buffers

BE02-017F BE02-017F PBS-EDTA 500 mL 500 mL 5) Buffers

12-747F 12-747F UltraSaline A without Phenol Red, 500 mL 500 mL 5) Buffers

10-548E 10-548E ACK Lysing Buffer 100 mL 100 mL 5) Buffers

10-539B 10-539B Dextrose Gelatin Veronal Buffer, 500 mL 500 mL 5) Buffers

17-512Q/12 BE17-512Q/12 DPBS 0.0095M(PO4) w/o Ca and Mg, 12 × 1 L 12 × 1L 5) Buffers

17-512Q BE17-512Q DPBS 0.0095M(PO4) w/o Ca and Mg, 1 L 1 L 5) Buffers

17-512F BE17-512F DPBS 0.0095M(PO4) w/o Ca and Mg, 500 mL 500 mL 5) Buffers

17-512F/12 BE17-512F/12 DPBS 0.0095M(PO4) w/o Ca&Mg, 12 × 500 mL 12 × 500 mL 5) Buffers

17-515Q BE17-515Q DPBS-10x w/o Ca, Mg 1 L 1 L 5) Buffers

17-515F BE17-515F DPBS-10X w/o Ca, Mg 500 mL 500 mL 5) Buffers

04-479Q 04-479Q DPBS-1X w/ Ca & Mg Glucose and Pyr, 1 L 1 L 5) Buffers

17-513Q BE17-513Q DPBS-1X w/ Ca, Mg 1 L 1 L 5) Buffers

17-513F BE17-513F DPBS-1X w/ Ca, Mg 500 mL 500ml 5) Buffers

N/A BE17-513F/12 DPBS-1X w/ Ca, Mg BOX of 12 bottles 12 × 500 mL 5) Buffers

17-512F/24 17-512F/24 DPBS-1X w/o Ca++, Mg++ 24 × 500 mL 24 × 500 mL 5) Buffers

N/A BE02-027F EBSS w/ HEPES low NaHCO3, 500 mL 500 mL 5) Buffers

N/A BE10-502F EBSS w/ Phenol Red 500ml 500 mL 5) Buffers

10-527F/12 10-527F/12 HANKS’ BSS, W/O Phenol Red 12x500ml 12 × 500 mL 5) Buffers

10-508Q 10-508Q Hanks’ BSS, with Phenol Red, 1 L 1 L 5) Buffers

10-508F BE10-508F HBSS w/ Phenol Red 500ml 500 mL 5) Buffers

10-543F BE10-543F HBSS w/ PR w/o Ca, Mg 500ml 500 mL 5) Buffers

N/A BE10-543F/12 HBSS w/ PR w/o Ca, Mg BOX of 12 bottles 12 × 500 mL 5) Buffers

10-527F BE10-527F HBSS w/o Phenol Red 500 mL 500 mL 5) Buffers

10-547F BE10-547F HBSS w/o PR, Ca or Mg 500 mL 500 mL 5) Buffers

04-315Q 04-315Q HBSS, w/o Ca++,Mg++ or Phenol Red, 1 L 1 L 5) Buffers

10-543Q 10-543Q HBSS, w/o Ca++,Mg++ w/ phenol red, 1 L 1 L 5) Buffers

BE17-737E 17-737E HEPES Buf,1M Stk in normal saline, 100 mL 100 mL 5) Buffers

17-737F 17-737F HEPES Buffer,1M in normal saline, 500 mL 500 mL 5) Buffers

17-517Q BE17-517Q PBS-10x w/o Ca, Mg 1 L 1 L 5) Buffers

17-516Q/12 BE17-516Q/12 PBS-1X w/o Ca, Mg 12 × 1 L 12 ×1 L 5) Buffers

17-516F/12 BE17-516F/12 PBS-1X w/o Ca, Mg 12 × 500 mL 12 × 500 mL 5) Buffers

17-516Q BE17-516Q PBS-1X w/o Ca, Mg 1 L 1L 5) Buffers

17-516F BE17-516F PBS-1X w/o Ca, Mg 500 mL 500 mL 5) Buffers

17-516F/24 17-516F/24 PBS-1X, w/o Ca, Mg 24 × 500 mL 24 × 500 mL 5) Buffers

17-724Q BE17-724Q USP WFI bulk Sterile filtered 1 L 1 L 5) Buffers

17-724F BE17-724F USP WFI bulk Sterile filtered 500 mL 500 mL 5) Buffers

12-624E 12-624E Veronal Buffer (5X) 100 mL 100 mL 5) Buffers

116



N/A BE17-724F/12 WFI Water-Cell Culture BOX of 12 bottles 12 × 500 mL 5) Buffers

N/A BE17-724Q/12 WFI Water-Cell Culture BOX of 12 bottles 12 × 1 L 5) Buffers

02-033E BE02-033E STD Insulin Human recomb. 5 mg/ml 100 mL 100 mL 6) Reagents

02-033E20 BE02-033E20 STD rh Insulin, 5mg/ml, 20ml bottle 20 mL 6) Reagents

02-033Q N/A STD Human Recombinant Insulin 5mg/ml, 1 L 1 L 6) Reagents

13-668C BE13-668C STD Lipids 2x concentr. 35 mL 35 mL 6) Reagents

13-668Z BE13-668Z STD Lipids 2x concentr. 7 mL 7 mL 6) Reagents

15-605G BE15-605G L-GLUTAMINE POWDER 25 G 25 g 6) Reagents

15-613I BE15-613I STD Sodium Bicarbonate powder 500 g 500 g 6) Reagents

BE02-033E80 N/A Human Rec Insulin 5 mg/ml 80 mL 80 mL 6) Reagents

BE02-058E BE02-058E STD Anti clumping agent A 100 mL 100 mL 6) Reagents

BE13-668F BE13-668F STD Lipids 2x concentr. 500 mL 500 mL 6) Reagents

BE13-668Z BE13-668Z STD Lipids 2x concentr. 7 mL 7 mL 6) Reagents

N/A BE15-605X(3000) STD L-Glutamine Powder 3000 g 3000 g 6) Reagents

BP12-769E BP12-769E ProFreeze-CDM, NAO, 2x Freeze Med, 100 mL 100 mL 6) Reagents

BP12-773Q BP12-773Q ProNS0 1 CD 1 L 6) Reagents

BP12-775J BP12-775J ProNS0™ Lipid CD 5 mL 6) Reagents

BP12-774Q BP12-774Q ProNSO 2 CD 1 L 6) Reagents

17-905C 17-905C L-Glutamine 200mM Hybridoma test, 50 mL 50 mL 6) Reagents

17-160F 17-160F Trypsin(10x) 2.5% /HBSS w/o Ca,Mg,500 mL 500 mL 6) Reagents

BE02-007E BE02-007E Trypsin/EDTA 10 × 100 mL 100 mL 6) Reagents

N/A BE02-034E Trypzean/EDTA solution 100 mL bottle 100 mL 6) Reagents

BE17-605E/U1 BE17-605E/U1 Ultraglutamine I 200mM 100 mL 100 mL 6) Reagents

17-836R 17-836R Amphotericin B,antifungal 250 ug/ml,20 mL 20 mL 6) Reagents

17-836E 17-836E Amphotericin B,antifungal 250ug/ml,100 mL 100 mL 6) Reagents

12-132A 12-132A Cryoprotective Freezing Medium, 100 mL 100 mL 6) Reagents

17-519Z 17-519Z Gentamicin 10 mg/ml 1 × 10 mL screw cap 10 mL 6) Reagents

17-519L 17-519L Gentamicin 10 mg/ml 10 × 10 mL screw cap 10 × 10 mL 6) Reagents

17-528Z 17-528Z Gentamicin 50 mg/ml 1 × 10 mL crimp-top 10 mL 6) Reagents

17-518Z 17-518Z Gentamicin 50 mg/ml 1 × 10 mL screw cap 10 mL 6) Reagents

17-518L 17-518L Gentamicin 50 mg/ml 10 × 10 mL screw cap 10 × 10 mL 6) Reagents

N/A BE02-012E Gentamycin Sulfate 100 mL, 10 mg/ml Gent 100ml 6) Reagents

17-839Z 17-839Z ITES (500 × stock) 5 mL 5 mL 6) Reagents

17-838Z 17-838Z ITS (500 × stock) 5 mL 5 mL 6) Reagents

17-605E BE17-605E L-Glutamine 200mM 100 mL 100 mL 6) Reagents

17-605F BE17-605F L-Glutamine 200mM 500 mL 500 mL 6) Reagents

17-605C 17-605C L-Glutamine 200mM 50 mL 50 mL 6) Reagents

N/A BE17-605E/12 L-Glutamine 200mM BOX of 12 bottles 12x100ml 6) Reagents

17-602E DE17-602E Pen 10.000 UI/ml Strep 10.000 UI/ml 100ml 6) Reagents

17-602F DE17-602F Pen 10.000 UI/ml Strep 10.000 UI/ml 500ml 6) Reagents

17-603E DE17-603E Pen 5.000 U/ml Strep 5.000 U/ml 100ml 6) Reagents

09-757F 09-757F Pen/Strep stock, 20K/20K 500 mL 500ml 6) Reagents

17-745E 17-745E Pen/Strep/Fungizone 10K/10K/25ug, 100 mL 100ml 6) Reagents

117



17-745H 17-745H Pen/Strep/Fungizone 10K/10K/25ug, 20 mL 20ml 6) Reagents

17-718R 17-718R Pen/Strep/L-Gln 25K/25K/200mM, 25x4.5 mL 25x4.5ml 6) Reagents

N/A DE17-603E/12 Penicillin/Strep 5000U/ml BOX of 12 BOT 12x100ml 6) Reagents

N/A DE17-602E/12 Penicillin10.000 UI/ml BOX of 12 bottles 12x100ml 6) Reagents

17-613E BE17-613E Sodium Bicarb. 7,5% sol 100ml 100ml 6) Reagents

13-115E BE13-115E Sodium Pyruvate Solution 100 mM, 100ml 100ml 6) Reagents

17-942E 17-942E Trypan Blue 0.4% 0.85% NaCl,100ml 100ml 6) Reagents

17-160E BE17-160E Trypsin(10x)2.5% in HBSS w/o Ca, Mg100ml 100ml 6) Reagents

N/A BE17-161E/12 Trypsin-Versene (EDTA) 12 × 100ml 12x100ml 6) Reagents

17-161E BE17-161E Trypsin-Versene (EDTA) Mix (1X) 100ml 100ml 6) Reagents

17-161F BE17-161F Trypsin-Versene (EDTA) Mix (1X) 500ml 500ml 6) Reagents

N/A BE17-605E/U1/12 Ultraglutamine I 200mM BOX of 12 bottles 12x 100ml 6) Reagents

N/A BE04-684E UltraGlutamine II 100ml 100ml 6) Reagents

N/A BE04-684E/12 UltraGlutamine II BOX of 12 bottles 12x100ml 6) Reagents

17-711E BE17-711E Versene (EDTA) 0.02% 100ml 100ml 6) Reagents

08-012B N/A STD RPMI-1640 w/o L-Gln 10 L Bag 10L 7) Classical Media

08-143F N/A STD IMDM W/O L-GLUT, 20L bag 20L 7) Classical Media

15-604D BE15-604D DMEM 4.5 g/L Glucose Powder, w/Gln, 10L 10L 7) Classical Media

15-702D 15-702D STD RPMI 1640 w/L-Gln Powder, 10L 10L 7) Classical Media

BE15-604F BE15-604F STD DMEM high glucose w/ L-Glutamine 50L 50L 7) Classical Media

N/A 08-858Q STD TheraPEAK™ DMEM KIT for HS-12 7) Classical Media

N/A BE15-375D STD EMEM w/oL-Glut,NaHCO3,NaH2PO4,PR 10L 10L 7) Classical Media

N/A BE15-375F STD EMEM w/oL-Glut,NaHCO3,NaH2PO4,PR 50L 50L 7) Classical Media

N/A BE15-375J STD EMEMw/oL-Glut,NaH2PO4,NaHCO3,PR 100L 100L 7) Classical Media

N/A BE15-375J300 STD EMEMw/oL-Glut,NaHCO3,NaH2PO4,PR 300L 300L 7) Classical Media

N/A BE15-398D STD RPMI 1640 w/o PR NaHCO3, L-Glut 10L 10L 7) Classical Media

N/A BE15-398F STD RPMI 1640 w/o PR NaHCO3, L-Glut 50L 50L 7) Classical Media

N/A BE15-398K STD RPMI 1640 w/o PR NaHCO3,L-Glut 1000L 1000L 7) Classical Media

N/A BE15-399D STD DMEM powder 10L 10L 7) Classical Media

N/A BE15-399F STD DMEM Powder 50L 50L 7) Classical Media

N/A BE15-399J STD DMEM Powder 100L 100L 7) Classical Media

N/A BE15-408D STD IMDM Powder w/o HEPES, PR L-Glut 10L 10L 7) Classical Media

N/A BE15-408F STD IMDM Powder w/o HEPES, PR L-Glut 50L 50L 7) Classical Media

N/A BE15-408J STD IMDM Pwd w/o HEPES, PR L-Glut 100L 100L 7) Classical Media

N/A BE15-408Q STD IMDM Pwd w/o HEPES, PR L-Glut 200L 200L 7) Classical Media

N/A BE15-604D STD DMEM high glucose w/l L-Glu 10L 10L 7) Classical Media

N/A BE15-611D MEM Eagle powder w/ EBSS - 10L 10L 7) Classical Media

N/A BE15-611F MEM Eagle powder w/ EBSS - 50L 50L 7) Classical Media

N/A BE15-611K MEM Eagle powder w/ EBSS - 1000L 1000L 7) Classical Media

N/A BE15-611X MEM Eagle powder w/ EBSS - vrac 7) Classical Media

BP77227 BP77227 HL-1 chemically defined FBS subst. 10 mL 10ml 7) Classical Media

BP77201 BEBP77201 HL-1 Kit 2x BE344017 Kit 7) Classical Media

BP344022 N/A PC-1 Supp 10mL A Component of 77232 10ml 7) Classical Media

118



BP77232 BP77232 PC-1 w/o Gln 2x500 mL 2x344018; 2x344022 2x500ml 7) Classical Media

BP12-743F BEBP12-743F UltraMEM w/ ITES 500 mL 500ml 7) Classical Media

12-615Q 12-615Q Ham’s F-12 Medium w/ L-Gln, 1L 1L 7) Classical Media

BE02-002F BE02-002F Alpha MEM Eagle w/ UGln1 & nucleo,500 mL 500ml 7) Classical Media

BE12-604F/U1 BE12-604F/U1 DMEM 4.5g/L Glucose w/ UltraGlut I 500ml 500ml 7) Classical Media

BE04-687F/U1 BE04-687F/U1 DMEM:F12 1: 1 w/ UGln 1, 500ml 500ml 7) Classical Media

BE04-687Q BE04-687Q DMEM:F12 1:1 Mixture 1L 1L 7) Classical Media

BE12-668F BE12-668F EMEM (2x) w/o L-Glu, PR 500 mL bottle 500ml 7) Classical Media

12-736E 12-736E MEM Eagle EBSS, Maint., w/FBS+, 100 mL 100ml 7) Classical Media

N/A BE12-169F/12 Alpha MEM Eagle w/o L-Gln BOX of 12 BOT 12x500ml 7) Classical Media

12-169F BE12-169F Alpha MEM Eagle w/o L-Glutamine 500ml 500ml 7) Classical Media

12-105F BE12-105F BME w/ EBSS w/o L-Glutamine, 500ml 500ml 7) Classical Media

12-709F BE12-709F DMEM 4,5g/L Glu,25mM HEPE,w/oL-Gln 500ml 500ml 7) Classical Media

N/A BE12-709F/12 DMEM 4,5g/L Gluc,25mM BOX of 12 bottles 12x500ml 7) Classical Media

12-733Q 12-733Q DMEM 4.5 g/L Gluc w/o L-Gln or Pyr, 1 L 1L 7) Classical Media

12-604Q/12 12-604Q/12 DMEM 4.5 g/L Glucose w/ L-Gln, 12 × 1L 12x1L 7) Classical Media

12-604Q 12-604Q DMEM 4.5 g/L Glucose w/ L-Gln, 1L 1L 7) Classical Media

12-614Q 12-614Q DMEM 4.5 g/L Glucose w/o L-Gln, 1 L 1L 7) Classical Media

12-614Q/12 12-614Q/12 DMEM 4.5 g/L Glucose w/o L-Gln, 12 × 1 L 12x1L 7) Classical Media

12-604F/12 BE12-604F/12 DMEM 4.5g/L Glucose w/ L-Glut 12 × 500ml 12x500ml 7) Classical Media

12-604F BE12-604F DMEM 4.5g/L Glucose w/ L-Glutamine 500ml 500ml 7) Classical Media

N/A BE12-604F/U1/12 DMEM 4.5g/L Glucose w/ U1 BOX of 12 BOT 12x500ml 7) Classical Media

12-917F BE12-917F DMEM 4.5g/L Glucose w/o L-Gln, PR, 500ml 500ml 7) Classical Media

N/A BE12-741F/12 DMEM high glucose BOX of 12 bottles 12x500ml 7) Classical Media

12-741F BE12-741F DMEM high glucose w/o Na Pyruvate 500ml 500ml 7) Classical Media

12-708F BE12-708F DMEM w/ 1,0 g/l Gluc w/ 25mM Hepes,500ml 500ml 7) Classical Media

N/A BE12-708F/12 DMEM w/ 1,0 g/l Gluc,25mM Hepes,12x500ml 12x500ml 7) Classical Media

12-614F/12 BE12-614F/12 DMEM w/ 4.5g/L gluc w/o L-Glut 12x500ml 12x500ml 7) Classical Media

12-614F BE12-614F DMEM w/ 4.5g/L glucose w/o L-Glut 500ml 500ml 7) Classical Media

N/A BE12-733F/12 DMEM w/ 4.5g/L glucose,BOX of 12 bottles 12x500ml 7) Classical Media

N/A BE12-707F/12 DMEM w/1,0 g/L glucose BOX of 12 bottles 12x500ml 7) Classical Media

12-707F BE12-707F DMEM w/1,0 g/L glucose w/o L-Glut, 500ml 500ml 7) Classical Media

12-733F BE12-733F DMEM w/4.5g/L gluc w/o L-Gln, Na Pyruvat 500ml 7) Classical Media

N/A BE12-719F/12 DMEM:F-12 1:1 Mixture BOX of 12 bottles 12x500ml 7) Classical Media

12-719F BE12-719F DMEM:F-12 1:1 w/15mM Hepes, L-Gln 500ml 500ml 7) Classical Media

12-719Q 12-719Q DMEM:F12 1:1 with HEPES, L-Gln, 1 L 1L 7) Classical Media

12-914F BE12-914F DMEM4.5g/L Gluc w/oL-Gln Hybrid tst 500 mL 500 mL 7) Classical Media

12-668E 12-668E EMEM EBSS (2x), w/o Gln, Phen Red,100 mL 100 mL 7) Classical Media

12-662Q 12-662Q EMEM EBSS w/ NEAA, Pyr, w/o L-Gln, 1 L 1 L 7) Classical Media

12-739F BE12-739F Glasgow-MEM w/ L-Glutamine 500 mL 500 mL 7) Classical Media

12-618F BE12-618F Ham’s F-10 Medium w/ L-Glutamine 500 mL 500 mL 7) Classical Media

N/A BE02-014F Ham’s F10 w/ UGln I 500 mL, w/o Thymidine 500 mL 7) Classical Media

119



12-615F BE12-615F Ham’s F-12 w/ L-Glutamine 500 mL 500 mL 7) Classical Media

N/A BE12-615F/12 Ham’s F-12 w/ L-Glutamine BOX of 12 BOT 12 × 500 mL 7) Classical Media

N/A BE12-618F/12 Ham’sF-10 Medium w/ L-Gln BOX of 12 BOT 12 × 500 mL 7) Classical Media

12-722F BE12-722F IMDM w/ 25 mM HEPES 500 mL 500 mL 7) Classical Media

N/A BE12-722F/12 IMDM w/ 25 mM HEPES BOX of 12 bottles 12 × 500 mL 7) Classical Media

12-915F BE12-915F IMDM w/ L-Glutamine w/ 25mM HEPES 500 mL 500 mL 7) Classical Media

12-726F BE12-726F IMDM w/o L-Glut w/25 mM HEPES, 500 mL 500 mL 7) Classical Media

12-726F/12 BE12-726F/12 IMDM w/o L-Glutamine BOX of 12 bottles 12 × 500 mL 7) Classical Media

12-722Q 12-722Q IMDM with HEPES and L-Gln, 1 L 1 L 7) Classical Media

12-726Q/12 12-726Q/12 IMDM with HEPES w/o L-Gln 12 × 1 L 12 × 500 mL 7) Classical Media

12-726Q 12-726Q IMDM with HEPES w/o L-Gln, 1 L 1 L 7) Classical Media

12-700Q 12-700Q L-15 (Leibovitz), without L-Gln, 1 L 1 L 7) Classical Media

12-700F BE12-700F L-15 (Leibovitz), without L-Gln, 500 mL 500 mL 7) Classical Media

12-669E 12-669E L-15(2x) w/o L-Gln or Phenol Red, 100 mL 100 mL 7) Classical Media

N/A BE12-700F/12 L-15(Leibovitz) w/o L-Glutamine 12 × 500 mL 12 × 500 mL 7) Classical Media

N/A BE02-015E Lymphochrome w/L-Gln w/ Gent PHA-P 100 mL 100 mL 7) Classical Media

12-688F BE12-688F McCoy’s 5A Medium w/ L-Glutamine 500 mL 500 mL 7) Classical Media

12-168F BE12-168F McCoy’s 5A w/ Gln w/ 25mM Hepes 500 mL 500 mL 7) Classical Media

12-118F BE12-118F Med 199 w/ HBSS,L-Gln, w/25mM Hepe 500 mL 500 mL 7) Classical Media

12-117F BE12-117F Med 199 w/EBSS, L-Gln w/25 mM Hepe 500 mL 500 mL 7) Classical Media

12-117Q 12-117Q Med199 w/EBSS,Gln, HEPE2.2g/L NaHCO3 1 L 1 L 7) Classical Media

N/A BE12-117F/12 Med199 w/EBSS,L-Gln,HEPES BOX of 12 BOT 12 × 500 mL 7) Classical Media

12-119F BE12-119F Medium 199 w/ EBSS 500 mL 500 mL 7) Classical Media

12-109F BE12-109F Medium 199 w/ HBSS 500 mL 500 mL 7) Classical Media

12-611Q 12-611Q MEM Eagle EBSS w/ L-Gln 1 L 1 L 7) Classical Media

12-611Q/12 12-611Q/12 MEM Eagle EBSS w/ L-Gln 12 × 1 L 12 × 1L 7) Classical Media

12-125Q 12-125Q MEM Eagle EBSS w/o L-Gln 1 L 1 L 7) Classical Media

12-125Q/12 12-125Q/12 MEM Eagle EBSS w/o L-Gln 12 × 1 L 12 × 1 L 7) Classical Media

12-736F 12-736F MEM Eagle EBSS, Maint., w/FBS+, 500 mL 500 mL 7) Classical Media

12-137F 12-137F MEM Eagle HBSS w/o L-Gln w/HEPES, 500 mL 500 mL 7) Classical Media

04-719Q 04-719Q MEM Eagle Joklik, w/L-Gln w/o Ca++, 1 L 1 L 7) Classical Media

13-114E BE13-114E MEM eagle NEAA (100X) 100 mL 100 mL 7) Classical Media

13-607C 13-607C MEM Eagle Vitamin mix (100x), 50 mL 50 mL 7) Classical Media

12-125F/12 BE12-125F/12 MEM Eagle w/EBSS, w/o L-Gln, 12 × 500 mL 12 × 500 mL 7) Classical Media

12-125F BE12-125F MEM Eagle with EBSS w/o L-Gln, 500 mL 500 mL 7) Classical Media

12-684F BE12-684F MEM Eagle-EBSS (10x) w/o L-Gln, 500 mL 500 mL 7) Classical Media

12-611F/12 BE12-611F/12 MEM Eagle-EBSS 12 × 500 mL 12 × 500 mL 7) Classical Media

12-611F BE12-611F MEM Eagle-EBSS 500 mL 500 mL 7) Classical Media

N/A BE12-662F/12 MEM Eagle-EBSS w/ NEAA BOX of 12 bottles 12 × 500 mL 7) Classical Media

12-662F BE12-662F MEM Eagle-EBSS w/ NEAA w/o L-Gln 500 mL 500 mL 7) Classical Media

06-174G BE06-174G MEM Eagle-EBSS with NEAA w/o CaCl2 450 mL 450 mL 7) Classical Media

12-127F BE12-127F MEM Eagle-HBSS w/o L-Gln, 500 mL 500 mL 7) Classical Media

120



N/A BE12-136F/12 MEM-Eagle-EBSS w/HEPES BOX of 12 bottles 12 × 500 mL 7) Classical Media

12-136Q BE12-136Q MEM-Eagle-EBSS w/HEPES w/o L-Glut 1 L 1 L 7) Classical Media

12-136F BE12-136F MEM-Eagle-EBSS w/HEPES w/o L-Glut 500 mL 500 mL 7) Classical Media

12-923E 12-923E NCTC109 w/EBSS,Gln hybridoma, 100 mL 100 mL 7) Classical Media

344018 344018 PC-1 Serum Free Media Component of 77232 500 mL 7) Classical Media

04-648F 04-648F Richter’s CM Medium 500 mL 500 mL 7) Classical Media

12-115Q/12 12-115Q/12 RPMI 1640 w/ 25mM HEPES 12 × 1 L 12 × 1 L 7) Classical Media

12-115F/12 BE12-115F/12 RPMI 1640 w/ 25mM HEPES 12 × 500 mL 12 × 500 mL 7) Classical Media

12-115F BE12-115F RPMI 1640 w/ 25mM HEPES 500 mL 500 mL 7) Classical Media

N/A BE12-115F/U1 RPMI 1640 w/ 25mM HEPES and U1 500 mL 500 mL 7) Classical Media

12-702Q 12-702Q RPMI 1640 w/ L-Gln 1 L 1 L 7) Classical Media

12-702Q/12 12-702Q/12 RPMI 1640 w/ L-Gln 12 × 1 L 12 × 1 L 7) Classical Media

BE12-752F BE12-752F RPMI 1640 w/ L-Glut w/o glucose 500 mL 500 mL 7) Classical Media

12-702F/12 BE12-702F/12 RPMI 1640 w/ L-Glutamine 12 × 500 mL 12 × 500 mL 7) Classical Media

12-702F BE12-702F RPMI 1640 w/ L-Glutamine 500 mL 500 mL 7) Classical Media

N/A BE12-702F/U1 RPMI 1640 w/ U1 500 mL 500 mL 7) Classical Media

N/A BE12-702F/U1/12 RPMI 1640 w/ U1 BOX of 12 bottles 12 × 500 mL 7) Classical Media

N/A BE12-115F/U1/12 RPMI 1640 w/ U1 BOX of 12 bottles 12 × 500 mL 7) Classical Media

12-167Q 12-167Q RPMI 1640 w/o L-Gln 1 L 1 L 7) Classical Media

12-167F/24 12-167F/24 RPMI 1640 w/o L-Gln 24 × 500 mL 24 × 500 mL 7) Classical Media

12-918F BE12-918F RPMI 1640 w/o L-Gln and PR (500 mL) 500 mL 7) Classical Media

12-167Q/12 12-167Q/12 RPMI 1640 w/o L-Glutamine 12 × 1L 12 × 1 L 7) Classical Media

12-167F/12 BE12-167F/12 RPMI 1640 w/o L-Glutamine 12 × 500 mL 12 × 500 mL 7) Classical Media

12-167F BE12-167F RPMI 1640 w/o L-Glutamine 500 mL 500 mL 7) Classical Media

12-115Q 12-115Q RPMI 1640 with HEPES and L-Gln, 1 L 1 L 7) Classical Media

N/A BE04-558F RPMI1640 w/ 25mM HEPES w/o Gln, 500 mL 500 mL 7) Classical Media

09-774E 09-774E RPMI1640,HEPES, Pen, Strep&Glut, 100 mL 100 mL 7) Classical Media

09-774F 09-774F RPMI1640,HEPES, Pen, Strep&Glut, 500 mL 500 mL 7) Classical Media

04-525F N/A STD RPMI1640w/MOPSw/L-Glnw/oNaHCO3,

500 mL

500 mL 7) Classical Media

BE02-019F BE02-019F William’s Med E w/o PR, L-Gln 500 mL 500 mL 7) Classical Media

N/A BE12-761F William’s Medium E w/o L-Gln 500 mL 500 mL 7) Classical Media

02-059E BE02-059E STD FeCitrate100x-PowerCHO™-2KIT,100 mL bt 100 mL Proteins + Reagents

BE02-040E BE02-040E Glucose stock solution 100g/L , 100 mL 100 mL Reagents + ProCHO +

PowerCHO™

121


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www.sartorius-stedim.comFor more information, you can contact us at [email protected]/media-buffer

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