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TEKNOLOGI DNA REKOMBINAN (KLONING)Posted on !"#!"$!! % & 'o((ents

Tekno)ogi *+ reko(binan, ata- )ebih dikena) dengan isti)ah .k)oning

*+/0 K)oning (er-pakan (etode yang dig-nakan -nt-k (e(b-at rep)ika

genetik dari sat- seg(en *+, se) ata- organis( secara kese)-r-han0

*engan de(ikian, k)oning *+ dig-nakan -nt-k (enghasi)kan kopi gen

ata- seg(en *+0 K)oning perta(a ka)i di)ak-kan ada)ah do(ba *o))y di

Skot)andia pada tah-n !112 yang (enghasi)kan do(ba yang identik

dengan ind-knya!0

K)oning do(ba *o))y di)ak-kan dengan somatic nuclear cell

transfer 3S+'T40 Se) so(atic do(ba 5antan dia(bi) inti se)nya ke(-dian

di(as-kkan da)a( se) te)-r do(ba betina yang s-dah dihi)angkan inti

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se)nya0 6enerasi perta(a (enghasi)kan do(ba berkepa)a hita( yang

(irip dengan ind-k betina sedangkan generasi ked-a (enghasi)kan

do(ba p-tih (irip dengan ind-k 5antan!0

*a)a( d-nia 7ar(asi, *+ reko(binan dig-nakan -nt-k prod-ksi protein7ar(asetik seperti ins-)in, hor(on pert-(b-han, inter7eron, 89g)ob-)in

dan (asih banyak )agi dapat di)ihat pada tabe) I diba:ah ini0 *a)a(

bidang )ain, tekno)ogi *+ reko(binan 5-ga dig-nakan -nt-k

(enghasi)kan he:an dan tana(an transgenic0 ;e:an transgenik

diharapkan dapat (e(p-nyai si7at9si7at yang diinginkan seperti tahan

terhadap penyakit sedangkan tana(an yang tahan terhadap penyakit

ata- (engand-ng vita(in"protein tertent-0 Tana(an transgenic perta(a

ka)i dipasarkan tah-n !11


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dengan en=i( retriksi endon-k)ease ke(-dian digab-ngkan ke(ba)i

dengan en=i( )igase0 *+ reko(binan ke(-dian di(as-kkan da)a( se)

bakteri s-paya dapat (enghasi)kan protein target yang diinginkan0

Tahap9tahap pe(b-atan *+ reko(binan>

a0 Pe(i)ihan vektor

b0 Pe(otongan *+ target dan vektor

c0 Penyisipan *+ target pada vektor

d0 Trans7or(asi

e0 Se)eksi hasi) trans7or(asi#

Re7erensi>

!0 +ES'O0 $nd ed0 ;-(an ')oning> Ethica) Iss-e0 $ an Introd-ction0 Aanchester, !11!

bo-t these adsRATE THIS:

! ote

This entry :as posted in CIDE S'IE+'E0 Book(ark the per(a)ink0

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6 RESPONSES TO “TENOLO#I !NA REOMBINAN $LONIN#%”

!0 Aree | 01/31/2011 at 3:31 PM | Reply

yg sya pernah baca, do))y (e(p-nyai -(-r yg pendek dibandingkan dgn

do(ba nor(a)0 (engapa?

o &ara(onina | 02/01/2011 at 6:42 AM | Reply

*o(ba *o))y p-nya -(-r yang pendek karena inti se) yang diba:a

ada)ah inti se) so(atik ind-k 5antannya0

Aengapa bisa de(ikian? *i da)a( inti se) terdapat *+ yang bagian

-5-ngnya dit-t-p o)eh pasangan basa ber-)ang

3te)o(er40 Pen-t-p -5-ng ini se(akin t-a se), se(akin pendek dan

sete)ah (encapai pan5ang (ini(-( (aka se) tidak

dapat (e(be)ah ke(-dian (ati0 +ah, pan5ang te)o(er *o))y (engik-ti

te)o(er ind-k 5antannya sehingga -(-rnya pendek0

$0 ed)isa*ada | 02/25/2011 at 3:23 PM | Reply

Se(oga 7ar(asi S* bisa sesegera (-ngkin p-nya (ata k-)iah prktik-(

ttg ha) inibanyak ide$ kreati7 pastinya akan ber(-nc-)ankeep Fre

on

o &ara(onina | 03/11/2011 at 5:03 AM | Reply

a(ien$:e are going to do this

(a- 5d pe)opor st-dy c)-b9nya?

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5/21

#0 ed)isa*ada | 04/06/2011 at 3:18 AM | Reply

se(oga bisa ber5a)an dengan baik b- rencana prktk-( biotek nya

hah saya ingn 7ok-s di k-)iah pt sa(a nyari$ in7o ttg bbrp prshaan b-0

Se(oga ada adik$ ke)as yg terdorong dan terinspirasi -tk (e(b-atnya

b- di tengah$ ker5aan k-)iah yg padat0 hehehehe06B0

o &ara(onina | 04/08/2011 at 12:24 PM | Reply

Ok00ttp s(angatGG

5gn )-pa nyari per-sahaan H posisi di(ana ka(- bs berke(bang

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Kamis, 27 Februari 2014

DNA Rekombinan

BAB I

PENDAHULUAN

A La!ar Be"akan#

Kema$uan %ibi%an# !ekno"o#i saa! ini su%a& berkemban# san#a! 'esa!, ban(ak'enemuan baru !en!an# bio"o#i mo"eku"ar, %ian!aran(a (ai!u a%an(a sis!em k"onin#

)is!em k"onin# i!u sen%iri meru'akan sua!u 'roses men#&asi"kan in%i*i%u+in%i*i%u %ari

$enis (an# sama i%en!ik seara #ene!ik Pa%a &e-an a!au !umbu&an !er!en!u

'en#k"onin#an !erben!uk seara a"ami (ai!u kebiasaan 'roses &e-an a!au !umbu&an

bere'ro%uksi aseksua" )e%an#kan %a"am bio!ekno"o#i, k"onin# meru$uk 'a%a berba#ai

Sign (e -p

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7/21

usa&a (an# %i"akukan manusia un!uk men#&asi"kan sa"inan berkas DNA a!au #en, se" a!au

or#anisme

)eirin# berkemban#n(a i"mu 'en#e!a&uan !ersebu!, munu""a& i"mu (an#

mem'e"a$ari men#enai 'emben!ukan kombinasi ma!eri #ene!ik (an# baru %en#an ara'en(isi'an mo"eku" DNA ke %a"am sua!u *ek!or se&in##a memun#kinkann(a un!uk

!erin!e#rasi %an men#a"ami 'erban(akan %a"am sua!u se" or#anisme "ain (an# ber'eran

seba#ai se" inan# (an# %ikena" seba#ai !ekno"o#i DNA rekombinan a!au biasa %isebu!

reka(asa #ene!ika .ekno"o#i ini memun#kinkann(a %i'ero"e&n(a sua!u 'ro%uk %en#an

si/a! !er!en!u %a"am -ak!u "ebi& e'a! %an $um"a& "ebi& besar %ari'a%a 'ro%uksi

seara kon*ensiona" ser!a mema%ukan si/a! %ari %ua $enis or#anisme (an# berbe%a

or#anisme !rans#enik %an "ain+"ain

Un!uk "ebi& $e"as men#enai DNA rekombinan, akan %i ba&as 'a%a 'emba&asanmaka"a& ini

B Rumusan asa"a&

Ber%asarkan "a!ar be"akan# %i a!as, maka %a'a! %irumuskan 'ermasa"a&an seba#ai

beriku! 3

1 A'aka& (an# %imaksu% DNA rekombinan

2 Ba#aimanaka& !eknik5!a&a'an !ekno"o#i DNA rekombinan

6 A'aka& man/aa! %ari !ekno"o#i DNA rekombinan

.u$uan

.u$uan %ari 'enu"isan maka"a& ini a%a"a& seba#ai beriku! 3

1 Un!uk men#e!a&ui 'en#er!ian %ari DNA rekombinan

2 Un!uk men#e!a&ui !eknik (an# %i#unakan %a"am !ekno"o#i DNA rekombinan

6 Un!uk men#e!a&ui man/aa! %ari !ekno"o#i DNA rekombinan


8/21

D an/aa!

an/aa! %ari 'enu"isan maka"a& ini a%a"a& seba#ai beriku! 3

1 Ba#i ma&asis-a %a'a! me"a!i& ke!eram'i"an %an kemam'uan %a"am membua! kar(a !u"is

i"mia&

2 Ba#i 'embaa %a'a! menamba& sumber in/ormasi !en!an# !ekno"o#i DNA rekombinan

BAB II

PEBAHA)AN

A De/inisi DNA Rekombinan


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enuru! o&en %an Bo(er 18903 DNA rekombinan rDNA a%a"a& ben!uk DNA

bua!an (an# %ibua! %en#an men##abun#kan %ua a!au "ebi& sekuens (an# !i%ak akan

biasan(a !er$a%i bersama+sama Da"am &a" mo%i/ikasi #ene!ik, i!u a%a"a& %ii'!akan

me"a"ui 'en#ena"an DNA (an# re"e*an ke %a"am DNA or#anisme (an# a%a, se'er!i

'"asmi% bak!eri, un!uk ko%e un!uk a!au men#uba& si/a! berbe%a un!uk !u$uan !er!en!u,se'er!i resis!ensi an!ibio!ik Ne-s e%ia"Ne!

Rober! 2008 men#a!akan ba&-a DNA rekombinan a%a"a& DNA (an# men#a"ami

'eruba&an karena 'en(isi'an sua!u sekuens %euksinuk"eo!i%a (an# sebe"umn(a !i%ak

!er%a'a! %a"am mo"eku" DNA (an# su%a& a%a %en#an ara en:ima!ik a!au kimia-i

Reka(asa #ene!ika a%a"a& seran#kaian !eknik un!uk memo%i/ikasi %an merekomen%asi

#en %ari berba#ai or#anisme (an# berbe%a (an# $u#a %isebu! !ekno"o#i DNA

rekombinan

.ekno"o#i (an# %ikena" seba#ai !ekno"o#i DNA rekombinan, a!au %en#an is!i"a&

(an# "ebi& 'o'u"er reka(asa #ene!ika, ini me"iba!kan u'a(a 'erban(akan #en !er!en!u %i

%a"am sua!u se" (an# bukan se" a"amin(a se&in##a serin# 'u"a %ika!akan seba#ai k"onin#

#en Ban(ak %e/inisi !e"a& %iberikan un!uk men%eskri'sikan 'en#er!ian !ekno"o#i DNA

rekombinan )a"a& sa!u %i an!aran(a, (an# mun#kin 'a"in# re'resen!a!i/, men(ebu!kan

ba&-a !ekno"o#i DNA rekombinan a%a"a& 'emben!ukan kombinasi ma!eri #ene!ik (an#

baru %en#an ara 'en(isi'an mo"eku" DNA ke %a"am sua!u *ek!or se&in##a

memun#kinkann(a un!uk !erin!e#rasi %an men#a"ami 'erban(akan %i %a"am sua!u se"

or#anisme "ain (an# ber'eran seba#ai se" inan# Reka(asa #ene!ika %a'a! memberikan

&asi" (an# san#a! men#un!un#kan )eba#ai on!o& 'asien (an# men%eri!a %iabe!es !i%ak

mam'u memben!uk &ormon insu"in un!uk men#a!ur ka%ar #u"a %a"am %ara& ;"e& karena

i!u, 'asien membu!u&kan sun!ikan insu"in seba#ai !amba&an Den#an !eknik reka(asa

#ene!ika, 'ara 'ene"i!i ber&asi" memaksa mikroor#anisme bak!eri un!uk memben!uk

insu"in (an# miri' %en#an insu"in manusia sur(o, 2001

B .ekno"o#i DNA Rekombinan

.ekno"o#i DNA rekombinan meru'akan !eknik ke$uru!eraan #ene!ik (an#

me"iba!kan 'en##unaan DNA rekombinan + (ai!u DNA (an# men##abun#kan mak"uma!#ene!ik %ua s'eies or#anisme (an# ber"ainan


10/21

1 ani'u"asi DNA in *i!ro "uar se" or#anisma

2 =abun#an, a!au rekombinasi DNA sesua!u or#anisma %en#an DNA bak!eria %a"am

'"asmi% a!au bak!erio/ak

6 Pen#k"onan, a!au !eknik un!uk mere'"ikasi 'ro#eni (an# memba-a DNA rekombinan

Proses+'roses %ia!as 'er!ama ka"i %i"akukan o"e& Pau" Ber# %an AD Kaiser 'a%a

!a&un 1872 ereka ber$a(a memasukkan DNA 'rokario! ke%a"am bak!eria, kemu%ian

o"e& )N o&en %an Herber! Bo(er (an# ber$a(a men##abun#kan DNA or#anisme

eukario! bersama '"asmi% bak!eria

Kom'onen (an# %i#unakan %a"am !eknik DNA rekombinan %ian!aran(a en:im

res!riksi un!uk memo!on# DNA, en:im "i#ase un!uk men(ambun# DNA %an *ek!or un!uk

men(ambun# %an men#k"onkan #en %i %a"am se" &i%u', !rans'oson seba#ai a"a! un!uk

me"akukan mu!a#enesis %an un!uk men(isi'kan 'enan%a, 'us!aka #enom un!uk

men(im'an #en a!au /ra#men DNA (an# !e"a& %ik"onkan, en:im !ranskri'si ba"ik un!uk

membua! DNA ber%asarkan RNA, 'e"aak DNA a!au RNA un!uk men%e!eksi #en a!au

/ra#men DNA (an# %iin#inkan a!au un!uk men%e!eksi k"on (an# benar >ek!or (an#

serin# %i#unakan %ian!arn(a '"asmi%, kosmi% %an bak!erio/a# En:im res!riksi,

%i#unakan un!uk memo!on# DNA En:im res!riksi men#ena" %an memo!on# DNA 'a%a

sekuens s'esi/ik (an# 'an$an#n(a em'a! sam'ai enam 'asan# basa En:im !ersebu!

%ikena" %en#an nama en:im en%onuk"ease res!riksi

A%a %ua ba#ian !er'en!in# (an# se"a"u %i#unakan %a"am reka(asa #ene!ika (ai!useba#ai beriku! 3

1 En:im se"u"er5e""u"ar En:(mes

En:im (an# %i'akai %a"am memani'u"asi DNA %ian!aran(a a%a"a& 3

a en:im En%onuk"ease, (ai!u en:im (an# men#ena"i ba!as+ba!as sekuen nuk"eo!i%a

s'esi/ik %an ber/un#si %a"am 'roses res!ri!ion a!au 'emo!on#an ba&an+ba&an #ene!ik

Pen##unaan en:im ini (an# 'a"in# umum an!ara "ain 'a%a sekuen 'a"in%romik En:im ini

%iben!uk %ari bak!eri (an# %ibua! se%emikian ru'a se&in##a %a'a! mena&an 'en(usu'anDNA, se'er!i #enom ba!erio'&a#e

b DNA 'o"imerisasi, (ai!u en:im (an# biasa %i'akai un!uk men#+o'( DNA En:im ini

men#sin!esis DNA %ari se" in%ukn(a %an memben!uk DNA (an# sama 'ersis ke se" in%uk

barun(a En:im ini $u#a bisa %i%a'a!kan %ari berba#ai $enis or#anisme, (an# !i%ak

men#&erankan, karena semua or#anisme 'as!i &arus men#+o'( DNA mereka


11/21

RNA 'o"imerisasi (ai!u en:im (an# ber/un#si un!uk ?membaa sekuen DNA %an

men#sin!esis mo"eku" RNA kom'"emen!er )e'er!i &a"n(a DNA 'o"imerisasi, RNA

'o"imerisasi $u#a ban(ak %i!emukan %i ban(ak or#anisme karena semua or#anisme &arus

?merekam? #en mereka

% DNA "i#ase meru'akan sua!u en:im (an# ber/un#si un!uk men(ambun#kan sua!u ba&an

#ene!ik %en#an ba&an #ene!ik (an# "ain on!o&n(a sa$a, en:im DNA "i#ase ini %a'a!

ber#abun# %en#an DNA a!au RNA %an memben!uk ika!an '&os'&o%ies!er baru an!ara

DNA a!au RNA (an# sa!u %en#an "ainn(a

e Re*erse !ransri'!ases a%a"a& en:im (an# ber/un#si memben!uk b"ue+'rin! %ari

mo"eku" RNA memben!uk DNA DNA kom'"emen!er En:im ini %ibua! %ari *irus RNA

(an# men#uba& #enom RNA mereka men$a%i DNA ke!ika mereka men#in/eksi inan#n(a

En:im ini biasa %i'akai ke!ika ber!emu %en#an #en eukario!ik (an# biasan(a !er'isa&+

'isa& men$a%i 'o!on#an kei" %an %i'isa&kan o"e& in!rons %a"am kromosom

2 >ek!or na!ura"5 Na!ura" >e!ors

)eba#ai sa"a& sa!u ara un!uk memani'u"asi DNA %i "uar se", 'ara i"mu-an %a"am

bio!ekno"o#i &arus bisa membua! sua!u !em'a! (an# kea%aann(a s!abi" %an ook

%en#an !em'a! DNA (an# %imani'u"asi )eka"i "a#i, a"am !e"a& memberikan so"usi %ari

masa"a& ini >ek!or %isini bisa %iar!ikan seba#ai a"a! (an# memba-a DNA ke %a"am se"

in%uk barun(a

A#ar sua!u me!o%e %a"am reka(asa #ene!ika %ian##a' ber&asi", %i %a"am *ek!or, DNA&asi" rekombinan se&arusn(a benar+benar &an(a %iba-a se!e"a& sebe"umn(a DNA

rekombinan %i#abun#kan %en#an DNA *ek!or me"a"ui en:im "i#ase Namun %i %a"am

*ek!or, DNA rekombinan !i%ak !ermu!asi "a#i memben!uk DNA %en#an si/a! baru

on!o& %ari *ek!or na!ura" %ari a"am a%a"a& '"asmi% %an *irus a!au

ba!erio'&a#e @i!ar!o, 200

A%a'un !eknik 'embua!an DNA rekombinan a%a"a& seba#ai beriku!3

1 .eknik men#iso"asi DNA

2 .eknik memo!on# DNA %en#an men##unakan en:im re!riksi en%onuk"ease

6 .eknik men##abun#5 men(ambun# DNA %en#an men##unakan en:im "i#ase

4 .eknik memasukkan DNA ke%a"am se" &i%u' *ek!or

>ek!or berkemban# %en#an se"eksi DNA (an# %ireka(asa


12/21

=ambar 13 mekanisme DNA Rekombinan

1 Iso"asi DNA (an# %ia-a"i %en#an me"akukan 'erusakan ser!a 'en#&i"an#an %in%in# se"Da"am 'roses ini %a'a! %i"akukan seara mekanis a!au'un %en#an ara en:ima!is)e!e"a& 'erusakan se" !e"a& %i"akukan, "an#ka& se"an$u!n(a a%a"a& 'e"isisan se" &a" ini

%a'a! %i"akukan %en#an men##unakan bu//er nonosmo!ik, ser!a %e!er$en (an# kua!se'er!i !ri!on +100 a!au %en#an so%ium %o%esi" su"/a! )D) Remukan se" (an#%iakiba!kan o"e& "isisn(a se" %ibuan# %en#an me"akukan sen!ri/u#asi se&in##a bias%ibe%akan an!ara ba#ian (an# rusak ser!a or#ane" !ar#e! (an# 'a%a ak&irn(a%i%a'a!"kan DNA (an# nan!in(a %i"akukan 'emurnian %en#an 'enamba&an amoniumase!a! %an a"o&o"

2 Pemo!on#an mo"eku" DNA, baik #enomik mau'un '"asmi% %i"akukan %en#an en:imres!rik

si A%a

%ua maam en:im res!riksi (aieu en:im res!riksi !i'e I %anen:im res!riksi !i'e II En:im res!riksi !i'e II mem'un(ai si/a!+si/a! umum(an# 'en!in# seba#ai beriku!3

a men#ena"i uru!an !er!en!u se'an$an# em'a! &in##a !u$u& 'asan# basa%i %a"am mo"

eku" DNA

b memo!on# ke%ua un!ai mo"eku" DNA %i !em'a! !er!en!u 'a%a a!au %i %eka!!em'a!

'en#ena"ann(a

men#&asi"kan /ra#men+/ra#men DNA %en#an berba#ai ukuran %an uru!anbasa

Ber%asarkan !em'a! &asi" 'emo!on#an, /ra#men+/ra#men DNAmemi"iki %ua maamu$un# (ai!u3

U$un# "en#ke! s!i,k( en% a!au u$un# ko&esi/, !er$a%i $ika !em'a!'emo!on#an

'a%a ke%ua un!ai DNA !er'isa& se$au& bebera'a 'asan# basa,se&in##a men#&asi"kan

/ra#men+/ra#men %en#an u$un# ? (an# runin#karena masin#+masin#

un!ai !un##a"n(a men$a%i !i%ak sama

'an$an# Dua/ra#men DNA %en#an u$un# (an# runin# akan mu%a& %isambun#kansa!u

sama "ain

U$un# !um'u" b"un! en% , !er$a%i $ika !em'a! 'emo!on#an DNA 'a%a'osisi (an# sama

Ke%ua /ra#men &asi" 'emo!on#ann(a akan mem'un(ai u$un# ? (an# !um'u" karena

masin#+masin# un!ai !un##a"n(a sama'an$an#n(a

Pen(a!uan %ua /ra#men DNA u$un# !um'u" su"i! %i"akukanse&in##a memer"ukan


13/21

'er"akuan !amba&an, misa"n(a 'emberian mo"eku""inker, mo"eku" a%a'!or, a!au 'enamba&

an en:im %eoksinuk"eo!i%i"!rans/erase

6 .a&a' se"an$u!n(a a%a"a& 'en(ambun#an mo"eku" DNA

A%a !i#a ara (an#%a'a! %i#unakan un!uk me"i#asi /ra#men+/ra#men DNA seara in*i!ro Per!ama,"i#asi men##unakan

en:im DNA "i#ase %ari bak!eri Ke%ua, "i#asi men##unakanDNA "i#ase %ari se"+se" E

,o"i (an# !e"a& %iin/eksi %en#an bak!erio/a# . 4 a!au"a:im

%isebu! seba#ai en:im . 4 "i#ase Ke!i#a (ai!u 'emberian en:im%eoksinuk"eo!i%i" !rans/e

rase un!uk men(in!esis un!ai !un##a" &omo'o"imerik 6?

)u&u o'!imum ba#i ak!i*i!as DNA "i#ase sebenarn(a

67C Akan !e!a'i, 'a%asu&u ini ika!an

&i%ro#en (an# seara a"ami !erben!uk %i an!ara u$un#+u$un#"en#ke!

akan men$a%i !i%ak s!abi" %an

kerusakan akiba! 'anas akan !er$a%i 'a%a!em'a! ika!an !ersebu! ;"e& karena i!u, "i#a

si biasan(a %i"akukan 'a%a su&uan!ara 4 %an 1C %en#an -ak!u inkubasi

reaksi (an# %i'er'an$an# serin# ka"i&in##a sema"am

4 )e!e"a& !a&a' 'en(ambun#an mo"eku"

DNA, %i"akukan ana"isis !er&a%a' &asi"'emo!on#an DNA #enomik %an DNA *ek!or ser!a

ana"isis &asi" "i#asi mo"eku"+mo"eku" DNA !ersebu! men##unakan !eknik e"ek!ro/oresis


14/21

(an#memba-a DNA rekombinan masi& &arus %i"akukan se"eksi

un!uk men%a'a!kanse" (an# DNA rekombinann(a memba-a /ra#men sisi'an a!au #en

(an#

%iin#inkan Pa%a %asarn(a a%a !i#a kemun#kinan (an# %a'a! !er$a%i se!e"a&!rans/ormasi

%i"akukan, (ai!u3

1 )e" inan# !i%ak %imasuki DNA a'a 'un a!au berar!i !rans/ormasi #a#a"

2 )e" inan# %imasuki *ek!or re"i#asi a!au berar!i "i#asi #a#a", %an

6 )e" inan# %imasuki *ek!or rekombinan %en#an5!an'a /ra#men sisi'an a!au#en

(an# %iin#inkan

)e"eksi se" rekombinan (an# memba-a /ra#men (an# %iin#inkan %i"akukan %en#an

menari/ra#men !ersebu! men##unakan /ra#men 'e"aak 'robe,(an# 'embua!ann(a %i"akukan

seara in

*i!ro men##unakan !eknik reaksi 'o"imerisasi beran!ai a!au 'o"(merase &ain rea!

ion PR

Pe"aakan/ra#men (an# %iin#inkan an!ara "ain %a'a! %i"akukan me"a"ui ara (an#%ina

makan &ibri%isasi ko"oni .$a&$o"eksono, 2008

.rans/er DNA a!au 'er'in%a&an DNA ke %a"am bak!eri %a'a! me"a"ui !i#a ara

(ai!u3

1 .rans/ormasi 3 !rans/er DNA (an# umumn(a berasa" %ari sa!u se" bak!eri ke %a"am se"

(an# berbe%a Prosesn(a a%a"a& ke!ika sebua& se" bak!eri 'ea& a!au "isis maka DNA

sirku"ar akan !er"e'as ke "in#kun#an E/isiensi !rans/ormasi ber#an!un# 'a%a

kom'e!ensi se"

2 Kon$u#asi 3 meru'akan 'emin%a&an ma!eri #ene!ik beru'a '"asmi% seara "an#sun#

me"a"ui kon!ak se" %en#an memben!uk s!ruk!ur se'er!i $emba!an %ian!ara %ua se"

bak!eri (an# ber%eka!an Umumn(a !er$a%i 'a%a bak!eri #ram ne#a!i/

6 .rans%uksi 3 !rans/er ma!eri #ene!ik %ari sa!u bak!eri ke bak!eri "ainn(a %en#an

men##unakan *irus bak!eri seba#ai *ek!or .rans/er ini men##unakan 'rinsi' %asar %ari

#a"ur %onor (an# men(e%iakan DNA ba#i #a"ur resi'ien Perbe%aan u!aman(a %en#an

!rans/er DNA "ainn(a a%a"a& DNA %i!rans/er me"a"ui 'eran!araan bak!erio/a#


15/21

an/aa! .ekno"o#i DNA Rekombinan

Berba#ai 'ene"i!ian !en!an# DNA rekombinan ban(ak #unakan %an %iman/aa!kan

%a"am berba#ai bi%an#, %ian!aran(a 3

1 Bi%an# in%us!ri

Pene"i!ian reka(asa #ene!ika %i bi%an# in%us!r( se%an# menin#ka! %en#an e'a!

Berba#ai usa&a (an# !e"a& #ia! %i"akukan misa"n(a 3

1 eni'!akan bak!eri (an# %a'a! me"aru!kan "o#am+"o#am "an#sun# %ari %a"am bumi

2 eni'!akan bak!eri (an# %a'a! men#&asi"kan ba&an kimia

eni'a!kan bak!eri (an# %a'a! men#&asi"kan ba&an men!a& kimia se'er!i e!i"en

(an# %i'er"ukan un!uk 'embua!an '"as!i

2 Bi%an# Per!anian

Bebera'a man/aa! reka(asa #ene!ika %a"am bi%an# 'er!anian %ian!aran(a a%a"a&3

1 en##an!i 'emakaian 'u'uk ni!ro#en (an# ban(ak %i'er#unakan !a'i ma&a" &ar#an(a,

o"e& /iksasi ni!ro#en seara a"amia& Bak!eri !ana& R&i:obium s' %a'a! men#a%akan

in/eksi ke %a"am akar %ari !anaman /ami"( Le#uminosae In/eksi ini men#&asi"kan bin!i"akar %an bak!eri (an# !er%a'a! %i %a"amn(a %a'a! men#ika! :a! "emas bebas %ari u%ara

un!uk %i uba&n(a men$a%i ni!ro#en (an# %a'a! %iambi" %an %i#unakan o"e& !anaman

!ersebu!

2 .eknik reka(asa #ene!ika men#usa&akan !anaman+!anaman !eru!ama (an# mem'un(ai

ar!i ekonomi (an# !i%ak be#i!u 'eka& !er&a%a' 'en(aki! (an# %isebabkan o"e& bak!eri,

$amur %an ain#

6 en#usa&akan !anaman+!anaman (an# mam'u men#&asi"kan 'e'!isi%a sen%iri

6 Bi%an# Pe!ernakan

1 .e"a& %i'ero"e& *aksin+*aksin un!uk me"a-an 'en(aki! menre! #anas (an# %a'a!

mema!ikan anak+anak babi


16/21

2 )u%a& %i'asarkan *aksin (an# e/ek!i/ !er&a%a' 'en(aki! kuku %an mu"u!, (ai!u

'en(aki! #anas %an san#a! menu"ar 'a%a sa'i, %omba, kambin#, rusa %an babi

4 Bi%an# Ke%ok!eran

=en+#en ba#i bebera'a 'ro!ein (an# %ibu!u&kan %a"am bi%an# ke%ok!eran (an#%ibu!u&kan %a"am bi%an# ke%ok!eran (ai!u 'embua!an insu"in manusia o"e& bak!eri

Es&re&ia o"i un!uk 'en#oba!an 'en(aki! %iabe!es @iki'e%iaor#

BAB III

PENU.UP

A Kesim'u"an

Ber%asarkan 'emba&asan 'a%a maka"a& ini maka %a'a! %i!arik bebera'a

kesim'u"an seba#ai beriku! 3

1 DNA rekombinan a%a"a& DNA (an# men#a"ami 'eruba&an karena 'en(isi'an sua!u

sekuens %euksinuk"eo!i%a (an# sebe"umn(a !i%ak !er%a'a! %a"am mo"eku" DNA (an#

su%a& a%a %en#an ara en:ima!ik a!au kimia-i

2 Kom'onen (an# !er"iba! %a"am Dna rekombinasi (ai!u En:im res!riksi un!uk memo!on#

DNA, DNA 'o"(merase, en:im "i#ase, en:im DNA "i#ase, >ek!or un!uk men(ambun# %an

men#k"onkan #en %i %a"am se" &i%u', .rans'oson, Pus!aka #enom, En:im !ranskri'si

ba"ik, Pe"aak DNA a!au RNA

6 .a&a'an+!a&a'an !ekno"o#i DNA rekombinan a%a"a& iso"asi DNA kromosom (an# akan

%ik"on, 'emo!on#an mo"eku" DNA men$a%i se$um"a& /ra#men %en#an berba#ai ukuran,

iso"asi DNA *ek!or, 'en(isi'an /ra#men DNA ke %a"am *ek!or un!uk men#&asi"kan

mo"eku" DNA rekombinan, !rans/ormasi se" inan# men##unakan mo"eku" DNA

rekombinan, reiso"asi mo"eku" DNA rekombinan %ari se" inan#, %an ana"isis DNA

rekombinan

B )aran


17/21

aka"a& ini masi& $au& %ari kesem'urnaann(a %an in/ormasin(a 'un !erba!as ;"e&

karena i!u, 'en(usun men(arankan a#ar 'embaa menari "i!era!ure "ain (an# memba&as

men#enai $u%u" %ari maka"a& ini

DAF.AR PU).AKA

Ans&ori DNA Rekombinan on"ine .erse%ia &!!'355ans&oriomu*om5

DNARekombinan&!m" .an##a" 1 No*ember 2012

I"&am 2008 DNA rekombinan on"ine .erse%ia &!!'355i"&am+

#an!:b"o#s'o!om520085065%na+rekombinan&!m" .an##a" 1 No*ember 2012

Ne-s e%ia", 2012 DNA Rekombinan .erse%ia &!!'355---ne-s+

me%ia"ne!5&ea"!&5Reombinan!+DNA+@&a!+is+Reombinan!+DNA+29In%onesian

28as'G .an##a" 1 No*ember 2012

.$a&$o"eksono, Aris, 2008 DNA Rekombinan ;n"ine .erse%ia&!!'355-ebi'bai%5+

!'b5/i"es5ma!eri5#ene!ika5%na5rekombinan&!m

http://anshori.comuv.com/%20DNA_Rekombinan.htmlhttp://anshori.comuv.com/%20DNA_Rekombinan.htmlhttp://ilham-gantz.blogspot.com/2009/03/dna-rekombinan.htmlhttp://ilham-gantz.blogspot.com/2009/03/dna-rekombinan.htmlhttp://www.news-medical.net/health/Recombinant-DNA-What-is-Recombinant-DNA-(Indonesian).aspxhttp://www.news-medical.net/health/Recombinant-DNA-What-is-Recombinant-DNA-(Indonesian).aspxhttp://www.news-medical.net/health/Recombinant-DNA-What-is-Recombinant-DNA-(Indonesian).aspxhttp://anshori.comuv.com/%20DNA_Rekombinan.htmlhttp://anshori.comuv.com/%20DNA_Rekombinan.htmlhttp://ilham-gantz.blogspot.com/2009/03/dna-rekombinan.htmlhttp://ilham-gantz.blogspot.com/2009/03/dna-rekombinan.htmlhttp://www.news-medical.net/health/Recombinant-DNA-What-is-Recombinant-DNA-(Indonesian).aspxhttp://www.news-medical.net/health/Recombinant-DNA-What-is-Recombinant-DNA-(Indonesian).aspxhttp://www.news-medical.net/health/Recombinant-DNA-What-is-Recombinant-DNA-(Indonesian).aspx


18/21

Ri/a?I, u&aimin P&De%) 2010 =ene!ika Rekombinasi %an Po'u"asi =a"aG( )iene a"an#

)a!riani 2011 DNA rekombinan on"ine

.erse%ia &!!'355sa!riani08n#eb"o#b"o#s'o!om520115125%na+rekombinan&!m" .an##

a" 1 No*ember 2012

@iki'e%ia 2011 Re,ombinan! DNA ;n"ine

.erse%ia&!!'355en-iki'e%iaor#5-iki5Reombinan!DNA .an##a" 1 No*ember 2012

@iki'e%ia 2011 .ekno"o#i DNA rekombinan ;n"ine

.erse%ia&!!'355ms-iki'e%iaor#5-iki5.ekno"o#iDNArekombinan .an##a" 1

No*ember 2012

@i!ar!o, AB, 200 Ins'ira!or Kema$uan I'!ek Pusa! Pene"i!ian Bio!ekno"o#i LIPI ;n"ine

.erse%ia &!!'355---beri!ai'!ekom5:beri!a+beri!ai'!ek+200.an##a" 1 No*ember

2012

Di'oskan o"e& Pu$o @inar!o %i 16

Kirimkan Ini "e-a! Emai" B"o#.&is Berba#i ke .-i!!erBerba#i ke FaebookBa#ikan ke

Pin!eres!

http://satriani09ngeblog/http://en.wikipedia.org/wiki/Recombinant_DNAhttp://ms.wikipedia.org/wiki/Teknologi_DNA_rekombinanhttp://www.beritaiptek.com/zberita-beritaiptek-2005https://plus.google.com/115748711375140916374http://catatannkuliahbiologi.blogspot.co.id/2014/02/dna-rekombinan.htmlhttps://www.blogger.com/share-post.g?blogID=2956093438734974330&postID=6708615787062431531&target=emailhttps://www.blogger.com/share-post.g?blogID=2956093438734974330&postID=6708615787062431531&target=emailhttps://www.blogger.com/share-post.g?blogID=2956093438734974330&postID=6708615787062431531&target=bloghttps://www.blogger.com/share-post.g?blogID=2956093438734974330&postID=6708615787062431531&target=bloghttps://www.blogger.com/share-post.g?blogID=2956093438734974330&postID=6708615787062431531&target=twitterhttps://www.blogger.com/share-post.g?blogID=2956093438734974330&postID=6708615787062431531&target=facebookhttps://www.blogger.com/share-post.g?blogID=2956093438734974330&postID=6708615787062431531&target=pinteresthttps://www.blogger.com/share-post.g?blogID=2956093438734974330&postID=6708615787062431531&target=pinteresthttp://satriani09ngeblog/http://en.wikipedia.org/wiki/Recombinant_DNAhttp://ms.wikipedia.org/wiki/Teknologi_DNA_rekombinanhttp://www.beritaiptek.com/zberita-beritaiptek-2005https://plus.google.com/115748711375140916374http://catatannkuliahbiologi.blogspot.co.id/2014/02/dna-rekombinan.htmlhttps://www.blogger.com/share-post.g?blogID=2956093438734974330&postID=6708615787062431531&target=emailhttps://www.blogger.com/share-post.g?blogID=2956093438734974330&postID=6708615787062431531&target=bloghttps://www.blogger.com/share-post.g?blogID=2956093438734974330&postID=6708615787062431531&target=twitterhttps://www.blogger.com/share-post.g?blogID=2956093438734974330&postID=6708615787062431531&target=facebookhttps://www.blogger.com/share-post.g?blogID=2956093438734974330&postID=6708615787062431531&target=pinteresthttps://www.blogger.com/share-post.g?blogID=2956093438734974330&postID=6708615787062431531&target=pinterest


19/21

.i%ak a%a komen!ar3

Poskan Komen!ar

Pos!in# Lebi& BaruBeran%aLan##anan3 Poskan Komen!ar A!om

Arsip Blog

2014 2! are! 1

Februari 1

DNA

Rekombinan

Mengenai Saya

Pujo Winarto

Li&a! 'ro/i" "en#ka'ku

http://catatannkuliahbiologi.blogspot.co.id/2014/03/bunga-buah-dan-biji.htmlhttp://catatannkuliahbiologi.blogspot.co.id/http://catatannkuliahbiologi.blogspot.com/feeds/6708615787062431531/comments/defaulthttp://void%280%29/http://catatannkuliahbiologi.blogspot.co.id/search?updated-min=2014-01-01T00:00:00-08:00&updated-max=2015-01-01T00:00:00-08:00&max-results=2http://void%280%29/http://catatannkuliahbiologi.blogspot.co.id/2014_03_01_archive.htmlhttp://void%280%29/http://catatannkuliahbiologi.blogspot.co.id/2014_02_01_archive.htmlhttp://catatannkuliahbiologi.blogspot.co.id/2014_02_01_archive.htmlhttp://catatannkuliahbiologi.blogspot.co.id/2014/02/dna-rekombinan.htmlhttp://catatannkuliahbiologi.blogspot.co.id/2014/02/dna-rekombinan.htmlhttps://plus.google.com/115748711375140916374https://plus.google.com/115748711375140916374http://catatannkuliahbiologi.blogspot.co.id/2014/03/bunga-buah-dan-biji.htmlhttp://catatannkuliahbiologi.blogspot.co.id/http://catatannkuliahbiologi.blogspot.com/feeds/6708615787062431531/comments/defaulthttp://void%280%29/http://catatannkuliahbiologi.blogspot.co.id/search?updated-min=2014-01-01T00:00:00-08:00&updated-max=2015-01-01T00:00:00-08:00&max-results=2http://void%280%29/http://catatannkuliahbiologi.blogspot.co.id/2014_03_01_archive.htmlhttp://void%280%29/http://catatannkuliahbiologi.blogspot.co.id/2014_02_01_archive.htmlhttp://catatannkuliahbiologi.blogspot.co.id/2014/02/dna-rekombinan.htmlhttp://catatannkuliahbiologi.blogspot.co.id/2014/02/dna-rekombinan.htmlhttps://plus.google.com/115748711375140916374https://plus.google.com/115748711375140916374


20/21


21/21

.em'"a!e .ra*e" Diber%a(akan o"e& B"o##er
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