biomolecular vibrational spectroscopy - rutgers university · 2009-03-31 · biomolecular...
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Biomolecular vibrational spectroscopy
Methods in Molecular Biophysics, Spring 2009
Basics of normal mode theoryAmide vibrations in proteins
Nuclear resonance vibrational spectroscopyTwo-dimensional vibrational spectroscopy
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Small molecule vibrational spectoscopy
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Amide vibrational spectra in proteins
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Deconvoluting complex spectra
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Deconvoluting complex spectra
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Fitting conformational preferences
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Fitting conformational preferences
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Complementary vibrational probes
cf. Guo, et al. Inorg. Chem. 47:3969, 2008
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Nuclear resonance vibrational spectroscopy
“vibrationalMossbauer”
only 57Fecontributes (nobackground)
intensity isproportion to KEin mode due toiron
“acoustic”librational modesvisible (inprinciple)
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Model compound IR spectra
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Model compound Raman spectra
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Model compound NRVS spectra
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Moving to a ferrdoxin protein
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Isotope shifts in the protein environment
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