biomedical research products · present in blood plasma. fibronectins are used to facilitate the...

Biomedical Technologies

• Lipoprotein Products• Cell Culture Products• Assay Kits & Components• Antibodies & Proteins• Biochemical Products

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BiomedicalResearch Products

Biomedical Technologies

Biomedical Technologies, (BT) is now part of Alfa Aesar. BT

has been manufacturing life science research products

for more than 30 years, with a commitment to customer

intimacy, high quality and reasonable prices. BT’s product

line includes specialty proteins, cell culture additives and

highly specific immunoassay tools which Alfa Aesar will

continue to build upon in expanding its portfolio of unique

biochemical products for the researcher.

Alfa Aesar is a leading manufacturer of fine chemicals

and catalysts used for research and development in a

wide span of applications. Alfa Aesar is already known

for providing researchers access to unparalleled expertise

and supply of a wide variety of inorganic and organic

chemicals used in both academia and industry.

Alfa Aesar is committed to enhancing our offering in the

biochemical arena through a selection of

methodically chosen products that complement our

current portfolio and build on our core competencies.

Protein research and characterization, all types of profiling

tools, and certain separation technologies are amongst such

products. Together, Alfa Aesar and BT continue to develop

and manufacture bio products to exceed our customers’

expectations.

Alfa Aesar currently offers a wide range of bio products in

the categories listed to the right.

Please visit www.alfa.com/biomedical-technologies for a full

range of products.

Alfa Aesar Offers:ACS Reagents

Amino Acids and Derivatives

Antibiotics

Antibodies

Apoptosis Reagents

Buffers

Carbohydrates and Derivatives

Cell Culture Reagents

Chiral Compounds

Click Chemistry Reagents

Electrophoresis Reagents

Enzymes

Growth Factors

Natural Products

Nucleosides and Nucleotides

Peptides

Signal Transduction Reagents

Solvents, Acids, Bases

Stains, Dyes and Indicators

Vitamins

Western Blot and ELISA Reagents

One Source, Globally

For more than 50 years, scientists have relied on

Alfa Aesar to supply high purity raw materials for a

variety of research and development

applications. Today we offer over 46,000 products

in stock, in sizes from gram-scale catalog items to

semi-bulk and bulk production quantities. With

custom manufacturing capabilities to supply many

more specialized items, we are a one-stop source

for research chemicals, metals and materials.

Alfa Aesar is a trusted, service-oriented company.

Product quality is of paramount importance, but production expertise is just the beginning. Factors such as product

availability, packaging and delivery are just as crucial to meeting and exceeding your expectations. Our established quality

process ensures a high level of service and a promise of continually striving for perfection.

Our dedicated teams, situated around the globe, are there to help you find the right products for your application when

you need them. Our experience in manufacturing, sourcing and handling a wide range of chemicals enables us to respond

quickly and efficiently to your needs.

Global InventoryThe majority of our 46,000 products are available in semi-bulk or bulk quantities from stock. Some are in regular production

and are available in bulk for next day shipment. We also supply most products from stock in research and development

quantities.

Global SourcingKnowledgeable chemical industry experts on our global sourcing team know what manufacturers match best to your

needs. All Alfa Aesar vendors are assessed for quality and service to remain on our approved vendor list. We practice

rigorous in-house product testing to ensure quality standards.

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Ward Hill,Massachusetts

Schiltigheim,France

Royston, England

Heysham, England

Karlsruhe, Germany

Hyderabad, India

Shanghai,China

Yantai,China

Seoul,Korea

Kandel, Germany

Taiwan(distributor)

Japan(distributor)

Singapore(distributor)

Reno, Nevada

(opening 2013)

Russia(distributor)

Cell Growth Factors

Endothelial Mitogen (ECGS)Endothelial Mitogen (ECGS) is a partially purified preparation from bovine hypothalamus. Endothelial Mitogen was originally prepared to facilitate the growth and survival of human (or mammalian) vascular endothelial cells in vitro. ECGS is now used to improve growth, supplement and/or reduce serum concentrations in cultures of endothelial cells, Balb/C-3T3 cells and hybridoma cell cloning. Optimal concentration range is from 25-200µg/ml and our ECGS does not contain streptomycin which may be toxic to cells. ECGS is tested for endotoxins and mycoplasma.

Stock No. Name Size

J64516 Endothelial Cell Growth Supplement, bovine hypothalamus

50mg10x50mg

Membrane filtered and packaged as a lyophilized solid

Epidermal Growth FactorEpidermal Growth Factor (EGF) is isolated from mouse submaxillary glands and is purified to electrophoretic homogeneity. Each lot is analyzed for radioreceptor binding, DNA replication (cell culture), immunological activity via RIA, and absolute purity via HPLC.

Stock No. Name Size

J65329 Epidermal Growth Factor, mouse submaxillary gland, 99%

100µg 10x100µg


Human Epidermal Growth FactorHuman Epidermal Growth Factor is recombinant DNA produced from E. coli. It is structurally identical (53 amino acids) to the natural Human EGF. Each lot is analyzed for radioreceptor binding, DNA replication (cell culture), immunological activity via RIA and absolute purity via HPLC.

Stock No. Name Size

J64012 Epidermal Growth Factor, human, Receptor Grade, 99%

50µg

J64008 Anti-human Epidermal Growth Factor antibody, from rabbit, whole serum

0.5ml


Rat Epidermal Growth FactorEGF from human and mouse sources is significantly different from rat EGF. Antibodies directed to human or mouse EGF do not recognize rat EGF sufficiently to adequately assay, localize or inhibit rat EGF containing samples. Receptor binding studies on rat cells are much “cleaner” using our Rat EGF reagents.

Stock No. Name Size

J64798 Epidermal Growth Factor, rat, Receptor Grade, 99%

10µg

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Cell Culture Products

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Basic Fibroblast Growth FactorBasic FGF, a Class II heparin-binding growth factor, is characterized by an isoelectric point 9.6 and elution from heparin-sepharose at 1.6-1.8M NaCl. bFGF has similar biological activities to aFGF, but mitogenicity is not potentiated by heparin. Bovine bFGF is purified (~95%, SDS-PAGE) from bovine pituitary glands via sepharose and ion exchange chromatography. Human bFGF (MW 17.3kDa) is produced in E. coli and purified to near homogeneity.

Stock No. Name Size

J65713 Fibroblast Growth Factor, basic, bovine, 95% 5µg25µg

J64811 Fibroblast Growth Factor, basic, human, 95% 10µg50µg

Membrane filtered and aseptically lyophilized

Insulin-like Growth FactorFactor-I and Factor-II Insulin-like Growth Factors are recombinant DNA produced. Both have full biological activity and are suitable for cell culture or receptor studies.

Stock No. Name Size

J64081 Insulin-like Growth Factor-I, human, 97% 10µg

J65170 Insulin-like Growth Factor-II, human, 97% 10µg

Anti-Nerve Growth FactorAnti-NGF is produced in rabbits from our mouse 2.5S NGF. The resulting antibody is purified by absorption. 90% inhibition of NGF (10ng/ml) activity was demonstrated on PC-12 cells at 1:100 dilution. Western blots of mouse 2.5S NGF (5ng) are detectable at 1:20 dilution of our anti-NGF using Goat Anti-Rabbit (GAR) alkaline phosphatase detection.

Stock No. Name Size

J64696 Anti-Mouse Nerve Growth Factor 2.5S antibody, from rabbit

0.5ml

Bovine Pituitary ExtractBovine Pituitary Extract (BPE) is an extract prepared from bovine pituitary glands. BPE is used in conjunction with other growth factors, hormones or media to reduce or eliminate serum in the culturing of some epithelial cell types. BPE has been effectively utilized in culturing normal human epidermal keratinocytes and other epithelial cells.

Stock No. Name Size

J64417 Bovine Pituitary Extract 50mg10x50mg


Platelet-Derived Growth FactorPlatelet-Derived Growth Factor BB is recombinant DNA produced and >97% pure via SDS gel electrophoresis. The acitivity of this product is similar to native h-PdGF in both receptor binding and in mitogen assays.

Stock No. Name Size

J64812 Platelet-Derived Growth Factor-BB, human, Receptor Grade, 97%

1µg

Transforming Growth Factor-alphaTransforming Growth Factor-alpha is produced by recombinant DNA. Each lot (>98% pure, HPLC) is evaluated for EGF receptor binding.

Stock No. Name Size

J64478 Transforming Growth Factor-alpha, human, 98%

2µg

Packaged as a lyophilized solid

Cell Growth Factors

Transforming Growth Factor-beta 1Transforming Growth Factor-beta 1 is isolated from blood bank produced human platelets. TGF-beta 1 is purified to >97% (SDS-PAGE).

Stock No. Name Size

J65044 Transforming Growth Factor-beta 1, human platelets, carrier free, 97%

5µg

Vascular Endothelial Growth FactorVascular Endothelial Cell Growth Factor (h-VEGF) is an angiogenesis factor produced by vascularized tissue. It is unique in that its activity is specific for endothelial cells and it has vascular permeability activity. VEGF (MW 38kDa) is a recombinant DNA produced dimer (>98%, SDS-PAGE) consisting of two 165 amino acid chains covalently linked via disulfide bond. Each lot is evaluated on bovine adrenal capillary endothelial cells for biological activity (6-10ng/ml).

Stock No. Name Size

J64375 Vascular Endothelial Cell Growth Factor, 98% 10µg

Packaged as a lyophilized solid

Other Growth FactorsStock No. Name Size

J64864 Nerve Growth Factor 2.5S, mouse submaxillary gland, 95%

10µg5x10µg

J65458 Brain Derived Acidic Fibroblast Growth Factor (102-111)

1mg

J64705 Epidermal Growth Factor Receptor Peptide (985-996)

1mg

J65662 Fibroblast Growth Factor Basic Fragment (1-24), bovine

0.5mg1mg

J61784 Growth Hormone Releasing Factor (GRF) (1-40), human

0.5mg1mg

J62895 Growth Hormone Releasing Factor (GRF) (1-44), human

0.5mg1mg

J65928 ß-Interleukin-I (163-171), human 1mg

J65203 ß-Interleukin-II (44-56) 1mg

J60485 Liver Cell Growth Factor 5mg

J64233 Liver Cell Growth Factor acetate salt 250mg

Cell Culture Additives

Hybridoma Cell Growth SupplementHybridoma Cell Growth Supplement (HCGS) is a conditioned media additive which significantly improves the cloning efficiency of hybridoma cells. HCGS eliminates the need for feeder cells.

Stock No. Name Size

J64856 Hybridoma Cell Growth Supplement 2x25ml

Platelet-Poor Plasma Derived SerumPlatelet-Poor Plasma Derived Serum is the first ready to use platelet-poor serum. It is prepared from fresh citrated bovine plasma and is essential in many mitogen cell culture assays. Endothelial cells can be cultured without contaminating cell types using this product.

Stock No. Name Size

J64483 Platelet-Poor Plasma Derived Serum, bovine 10ml100ml

Cell Culture Components

Stock No. Name Size

J60485 Liver Cell Growth Factor 5mg

J61321 Insulin, from porcine pancreas, 97% 25mg250mg

J61626 Transferrin (Apo), bovine plasma, 98+% 100mg500mg

1g

J64301 Goat Serum 5ml

J65195 Rabbit Serum 5ml


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Albumins

Stock No. Name Size

J65097 Albumin, Bovine, Cohn Fraction V, 98%, Cell Culture Grade, Low Endotoxin

10g50g

100g1kg

J64248 Albumin, Bovine, Fraction V, 35% soln., Cell Culture Grade, no preservative

50ml100ml

J64101 Albumin, Bovine, Fraction V, 35% soln., Cell Culture Grade, with azide as preservative

50ml100ml

J65030 Albumin, Bovine, New Zealand origin, Low Endotoxin, Cell Culture Grade, Chromatographically purified

10g25g50g

100g

Cell Attachment Factors

FibronectinFibronectin is a major cell surface glycoprotein and is also present in blood plasma. Fibronectins are used to facilitate the attachment of cells to a variety of surfaces and to maintain cells in low serum conditions. Fibronectin from three different species are purified from plasma to 95% (5% SDS-PAGE) and provided as a lyophilized solid. The Human Fibronectin ELISA Kit can effectively measure levels of human fibronectin to 50 nanograms per milliliter.

Stock No. Name Size

J62380 Fibronectin, bovine plasma, sterile-filtered 5mg

J64560 Fibronectin, human, 95% 1mg10x1mg

J64390 Fibronectin, rat, 95% 1mg

Membrane filtered and aseptically packaged

Anti-Fibronectin Antibodies

Stock No. Name Size

J64763 Anti-human Fibronectin antibody, from

rabbit, whole serum

1ml

Stabilized FibronectinMany lyophilized fibronectin preparations are difficult to solubilize (recoveries are only 85-95% at best) and after solubilization these preparations tend to aggregate and precipitate on standing. Our fibronectin solutions are completely solubilized and are stabilized to prevent precipitation. They retain full biological activity and are stable for 9 months stored at 4°C. Fibronectin solutions are convenient for tissue culture and can be used whenever accurately known quantities of fibronection are necessary.

Stock No. Name Size

J64738 Fibronectin, human, stabilized soln. 1mg10x1mg

J65696 Fibronectin, bovine, stabilized soln. 5mg

J65555 Fibronectin, rat, stabilized soln. 1mg

Membrane filtered and aseptically packaged

Anti-mouse Laminin IgG Anti-Laminin is produced in rabbits from mouse laminin. The antibody is purified by adsorption and is sold as an IgG fraction. It is suitable for immunohistochemical localization studies on frozen or paraffin sections, ELISA and immunoblots.

Stock No. Name Size

J65295 Anti-Mouse Laminin IgG antibody, from rabbit

0.5ml

Poly-D-LysinePoly-D-Lysine (30,000-70,000) is a synthetic peptide used to promote cell attachment. It is membrane filtered and aseptically lyophilized. Approximately 2µg of Poly-D-Lysine per square centimeter of plating surface is recommended.

Stock No. Name Size

J65578 Poly-D-lysine hydrobromide 5mg

Cell Culture Reagents

Our high purity cell culture reagents include cell culture media, laboratory preparations, biological extracts and sterile reagents.

Stock No. Name Size

J60279 L-Alanine, Cell Culture Reagent 50g100g500g

J62905 Calcium chloride, 100mM aq. Soln., Sterile 500ml1L

J63745 L-Cysteine, Cell Culture Reagent 25g100g

J62279 Erythromycin, Cell Culture Grade 10g25g

J62937 Folic acid, Cell Culture Reagent 5g25g

100g

J60067 D-(+)-Glucose, 1M aq. Soln., Sterile 125ml250ml

J60573 L-Glutamine, Cell Culture Reagent 25g100g1kg

J62399 Glycerol, Cell Culture Grade 500ml

J62407 Glycine, 99.5+%, Cell Culture Reagent 100g500g1kg

J62886 D-myo-Inositol, Cell Culture Grade 50g100g250g

J62575 Magnesium chloride hexahydrate, Cell Culture Reagent

250g500g1kg

J61014 Magnesium chloride, 1M aq. Soln., Sterile 125ml250ml

J61030 Magnesium sulfate, 1M aq. Soln., Sterile 125ml250ml

J61196 Phosphate-buffered saline (PBS, 1X), Sterile 500ml

J61807 Sodium chloride, 5M aq. Soln., pH 8.0, autoclaved

250ml500ml

J61840 Sodium pyruvate, Cell Culture Grade 50g100g500g

References1. Cybulsky, M.I. & Gimbrone, M.A., Science, 251, 788-791 (1991).2. Lopez-Casillas, F. et. al., Cell, 67, 785-795 (1991).3. Lobb et. al., JBC, 261, 1924, 1986.4. Burgess et. al., JBC, 260, 11389, 1985.5. Adamis, A.,Meklir B., & Joyce N., Am. J. Pathol., 139: 961-967 (1991).6. Tsao, M.C., et. al., J. Cell Physiol. 110: 219-229, 1982.7. Grove, R.I. et. al., J. Lipid. Res. 32: 1889-1897 (1991).8. Artalejo, C.R., Perlman R.L. & Fox A.P. Neuron 8: 85-95 (1992).

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Cyclic AMP & Cyclic GMP Enzyme Immunoassay Kits• Highly Sensitive• Dual Range• Same Day or Overnight Protocol• Non-Radioactive

Cyclic AMP and Cyclic GMP EIA Kits are complete systems. Each kit uses alkaline phosphatase labeled tracer (cAMP or cGMP) competing for sites on the antibody with standard or sample. Both assays employ a highly specific antibody to ensure accuratJe, consistent results. The assay sensitivities are outstanding. As little as 0.005 picomoles per well of cAMP or cGMP can be measured.

Levels of cAMP or cGMP can be conveniently measured from most biological samples (urine, plasma and cell culture) without the extensive purification procedures inherent in other methodologies.

Both the Cyclic AMP and Cyclic GMP assays can be run either overnight or in 6 hours. The protocol includes only two pipeting steps and one wash sequence per assay. Each kit accommodates 80 samples run in duplicate (or 160 wells for unknowns).

Stock No. Name Size

J65001 Cyclic AMP Enzyme Immunoassay Kit 1 kit

J65422 Cyclic GMP Enzyme Immunoassay Kit 1 kit

J64209 Cyclic GMP Enzyme Immunoassay Kit, without acetic anhydride

1 kit

J64184 Cyclic GMP Enzyme Immunoassay Kit, without acetic anhydride

1 kit

Each kit includes sufficient reagents and 2 precoated 96-well strips.Shelf life is 5 months stored a 4°C.

Involucrin Enzyme Immunoassay Kit• Keratinocyte Differentiation Marker• Highly Sensitive (0.05ng/well)• Non-Radioactive• Convenient

Involucrin, a soluble cytoplasmic protein with a highly repetitive amino acid sequence, serves as a substrate for transglutaminase cross-linking in the formation of cornified envelopes. Like the cross-linked envelope, involucrin expression is a specific marker for keratinocyte differentiation.

This protein is synthesized not only by the cells of the epidermis and other squamous epithelia, but also in epithelia undergoing squamous metaplasia and in certain tumors, as in the lung, which display epidermoid characteristics. In culture the growth and differentiation of keratinocytes, expecially those derived from tumors, can be modulated greatly by physiological (retinoids, calcium) and toxic agents (including carcinogens). The involucrin content of the cells is highly responsive to such treatments and thus its measurement by ELISA can serve as a convenient and sensitive indicator of the cellular response.

The Involucrin ELISA Kit is designed to provide a reproducible, quantitative method of measuring involucrin. The assay is highly sensitive, 0.05-0.5 nanograms per well. Levels of human involucrin can be directly measured after separation of cellular debris. A complete protocol is provided with each kit.

Stock No. Name Size

J64994 Involucrin, human, Enzyme Immunoassay Kit Contains:Highly purified Human InvolucrinAnti-Human Involucrin (rabbit)Goat anti-Rabbit IgG Alkaline Phosphatase

1 kit

Sufficient for 2500 tests or 25-96 well plates

Assay Kits & Components

Assay Kits & Components

Osteocalcin EIA Kits

Osteocalcin (MW 5.8kDa) is a specific product of the osteoblast. It is primarily deposited into the ECM of bone and only a small amount of newly synthesized osteocalcin is released directly into the circulation. Recent studies suggest that there are various forms of osteocalcin in the circulation and that different antibodies detect both intact and fragments of osteocalcin. The physiological significance of osteocalcin fragments is unclear but they may be derived from resorption, osteoblastic synthesis, systemic catabolism or all of these. Since serum proteases may also degrade the intact osteocalcin, it is recommended that blood samples be processed quickly in the presence of protease inhibitors.

Intact Human Osteocalcin Enzyme Immunoassay Kit• Defined Specificity - Measures Only Intact Osteocalcin• Highly Sensitive - only 25µl sample• Results in 3.5 hours• Non-Radioactive - Sandwich ELISA

The Intact Osteocalcin EIA measures only the intact osteocalcin which is synthesized de novo by the osteoblast and it eliminates any interference by circulating fragments. The assay is a sandwich EIA which employs two monoclonal antibodies directed towards the amino-and the carboxy-terminal regions of the human osteocalcin. It recognizes only intact osteocalcin, requiring the full 49 residue protein for detection.

The assay is highly sensitive (Range: 1-50ng/ml) and requires only a 25µl sample. A complete protocol is provided with each kit.

Stock No. Name Size

J64816 Osteocalcin, human, intact, Enzyme Immunoassay Kit

1 kit

Each kit includes sufficient reagents and a precoated 96-well strip.

Mid-Tact Human Osteocalcin Enzyme Immunoassay Kit• Defined Specificity - Measures Intact Osteocalcin Plus

the (1-43) Major Fragment• Highly Sensitive - only 25µl sample• Results in 3.5 hours• Non-Radioactive - Sandwich ELISA

The Mid-Tact Osteocalcin ELISA Kit is highly specific. It measures both the intact human osteocalcin and the major (1-43) fragment. The assay is a sandwich EIA which employs two monoclonal antibodies. One antibody (1-19) is immobilized in the wells and the second antibody (30-40) is biotinylated. The assay is highly sensitive (0.5ng/ml)and requires only a 25µl sample. A complete protocol isprovided with each kit.

Stock No. Name Size

J64764 Osteocalcin, human, mid-tact, Enzyme Immunoassay Kit

1 kit


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Mouse Osteocalcin Enzyme Immunoassay Kit• Sandwich ELISA• Requires only 5-10µl Serum• Non-Radioactive

The Mouse Osteocalcin EIA kit is a sandwich ELISA assay. Two mouse osteocalcin antibodies are employed, each directed toward an end (C or N terminal) of the osteocalcin molecule. One mouse osteocalcin antibody is bound to the wells which binds the mouse osteocalcin standard or sample, the other biotinylated antibody completes the sandwich.

This sandwich ELISA kit is specific for intact mouse osteocalcin only. Both carboxylated and decarboxylated mouse osteocalcin are recognized. Mouse Osteocalcin can be measured at a 1:10 to 1:20 dilution from serum or heparinized plasma. Bone extracts and serum-free cell culture supernates can be measured directly.

The protocol has an overnight incubation and yields a1-50ng/ml range. As little as 5-10µl of mouse serum isrequired for duplicate determinations. A complete protocolis provided with each kit.

Stock No. Name Size

J64239 Mouse Osteocalcin Enzyme Immunoassay Kit

1 kit


Rat Osteocalcin Enzyme Immunoassay KitThe Rat Osteocalcin EIA Kit is a sandwich ELISA assay specific for rat osteocalcin only. The N-terminal monoclonal antibody is bound to the wells and then reacted with rat osteocalcin standard or sample. The C-terminal rat osteocalcin antibody completes the sandwich. Both carboxylated and decarboxylated rat osteocalcin are recognized. Rat osteocalcin can be measured directly from serum, heparinized plasma, bone extracts and serum-free cell culture supernates.

The assay is run in 5 hours and yields a 0.33-20ng/ml range. As little as 10 μl of rat serum is required for duplicate determinations. A complete protocol is provided with each kit.

Stock No. Name Size

J65214 Rat Osteocalcin Enzyme Immunoassay Kit 1 kit


References1. Hess L.W., O’Brien W.F., Holmberg, J.A., Winkel C.A., Monaghan W.P., and Hemming V.G. Plasma and Amniotic Fluid Concentration of Fibronection During Normal Pregnancy. Obstetrics and Gynecology, 68: 25-28 (1986).2. Eielson C., Kaplan D., Mitnick M.A., Paliwal I., and Insogna K. Estrogen Modulates PTH-Induced Fibronectin Production in Human and Rat Osteoblast-like Cells. Endocrinology 135, No. 4: 1639-1644 (1994).3. Walts, A.E. et al (1985) Involucrin, a marker of squamous and urothelial differentiation. An immunohistochemical study on its distribution in normal and neoplastic tissues. J. Pathol. 145: 329-340.4. Eckert, R.L. and Green, H. (1986) Structure and evolution of the human involucrin gene. Cell 46: 583-589.5. Parenteau, N.L. et al (1987) Primate Involucrins: Antigenic relatedness and detection of multiple forms. Proc. Natl. Acad. Sci. USA 84: 7571-7575.6. Rice, R.H. et al (1988) Suppression of keratinocyte differentiation in SCC-9 human squamous carcinoma celss by benzo(a)pyrene, 12-O-tetradecanoyl phorbol 13 acetate and hydroxyurea. Carcinogenesis 9: 1885-1890.7. Rubin, A.L. et al (1989) Coordination of keratinocyte programming in human SCC-13 squamous carcinoma and normal epidural cells. J. Cell. Physiol. 138: 208-214.8. Gaido, K.W. and Maness, S.C. (1994) Regulation of Gene Expression and Acceleration of differentiation in Human Keratinocytes By TCDD. Toxicol, & Applied Pharm. 127: 199-208.9. Gundberg C.M., Weinstein R.S. Multiple immunoreactive forms of osteocalcin in uremic serum. J. Clin Invest 77: 1762-67, 1986.10. Power M.J., Gosling J.P., Fotrell P.F. Radioimmunoassay of osteocalcin with polyclonal and monoclonal anitbodies. Clin Chem 35: 1408-15, 1989.11. Calvo, M.S., Eyre D.R., and Gundberg C.M. Molecular Basis and Clinical Application of Biological Markers of Bone Turnover. Endocrine Reviews 17 (4): 333-364, 1996.12. Bodine, P.V.N., Trailsmith, M. and B.S. Komm. Development and Characterization of a Conditionally Transformed Adult Human Osteoblastic Cell Line. J. of Bone & Miner. Res. 11: 806-819, 1996.13. Nutall, M.E., Patton, A.J., Olivera, D.L., Nadeau, D.P., & M. Gowen. Human Trabecular Bone Cells are able to Express Both Osteoblastic and Adipocytic Phenotype: Implications for Osteopenic Disorders. J. of Bone & Miner. Res. 13: 371-382, 1998.14. Garnero P., Grimaux M., Seguin P. and P.D. Delmas. Characterization of Immunoreactive Forms of Human Osteocalcin in vivo and in vitro. J. of Bone & Mineral Res. 9 (2): 255-264, 1994.15. Tsuji, H.,Cawthorne,C. and B.Ecarot. Abnomal Modulation of Serum Osteocalcin by Dietary Phosphate and 1,25-Dihydroxyvitamin D3 in the Hypophosphatemic Mouse. Journal of Bone and Mineral Research 11: 1234-1240 (1996).17. Wenstrup, R. J.,Fowlkes, J. L., Witte, D.P. and J.B. Florer. Discordant Expression of Osteoblast Markers in MC3T3-E1 Cells that Synthesize a High Turnover Matrix. The Journal of Biological Chemistry. 271: 10271-10276 (1996).18. Kousteni, S. et al. Reversal of Bone Loss in Mice by Nongenotropic Signaling of Sex Steroids. Science. 298: 843-846 (2002).

Antibodies & Proteins

Stock No. Name Application Size

J64001 Anti-cyclic AMP antibody, from rabbit Unconjugated antibody. Suitable for radioimmunoassays. 100each1000each

J64025 Anti-cyclic GMP antibody, from rabbit Unconjugated antibody. Suitable for radioimmunoassays 100each1000each

J64967 Anti-Rabbit IgG-FITC from goat A monospecific IgG suitable for immunohistochemical applications

1ml

J65031 Anti-Rabbit IgG-TRITC, from goat Adsorbed. Suitable for immunohistochemical applications 1ml

J65418 Anti-Myosin IIA (non-muscle) Heavy Chain antibody, from rabbit

Suitable for immunohistochemical applications and Western blotting.

0.5ml

J64155 Anti-Rabbit IgG-Peroxidase, from goat Affinity purified. Suitable for immunohistochemical applications and Western blotting

1ml

J64787 Anti-Mouse IgG-Peroxidase, from goat Adsorbed. Suitable for enzyme immunoassays, immunohistochemistry and Western blotting

1mg

J64160 Anti-Rabbit IgG, from goat Precipitating antibody 5ml

J65216 Anti-Mouse Osteocalcin antibody, from goat, whole serum

Suitable for immunohistochemical applications. Lyophilized from whole serum

0.25ml

Antibodies

An antibody is a large Y-shaped protein that is used by the immune system to identify and neutralize foreign objects such as bacteria and viruses. Using a binding mechanism, an antibody can tag a microbe or an infected cell for attack by other parts of the immune system, or can neutralize its target directly. Alfa Aesar offers antibodies to the cytoskeletal proteins, growth & attachment factors, lipoproteins, hormones and keratinocyte differentiation markers are also offered. Most of the antibodies are provided lyophilized or in buffer containing 0.02% sodium azide.

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Antibodies

Anti-Involucrin Antibody Kit In stratified squamous epithelia of human skin, esophagus, vagina and conjunctiva, involucrin is absent in basal cells and is synthesized by the maturing superficial cells. The protein is a marker for normal suprabasal squamous differentiation. Involucrin is also found in urothelium, thymic Hassall’s corpuscles, ducts of skin appendages and regions of squamous metaplasia in lung, pancreas, prostate and uterine cervix.

Human squamous carcinomas of epidermis and oral cavity are defective in involucrin synthesis in culture, and such lesions show corresponding alteration of involucrin staining in vivo. In advanced neoplasms of skin, larynx and bladder, for example, the staining is often patchy, reflecting the evident cellular disorganization. Such patterns are also visible in carcinomas showing squamous differentiation in the lung, endometrium and other locations. In dysplasias of uterine cervix, involucrin staining often is reduced. These results indicate that involucrin staining may be useful in characterizing certain premalignant and malignant lesions, detecting regions of squamous differentiation and determining histogenesis of selected neoplasms.

Our Involucrin Antibody Kit consists of highly specific Involucrin antibody (rabbit) and other reagents to peroxidase stain Involucrin in tissue sections. A complete sample protocol is included with each 100 section kit.

Stock No. Name Size

J65602 Anti-Involucrin Antibody KitContains:Anti-Human Involucrin (rabbit)Goat anti-rabbit IgG-HRP3,3’-Diaminobenzidine HClNormal Goat Serum

1 kit (100 sections)

Should be refrigerated at 4°C and is stable for 6 months.

Natural Products

Stock No. Name Size

J60364 Chenodeoxycholic acid 5g25g

J62050 Cholic acid sodium salt 25g100g250g

J60218 Collagen, bovine achilles tendon 1g5g

10g

J62122 Cytochrome C, equine heart, 90+% 250mg1g

J62288 Sodium deoxycholate, 97% 25g100g250g

J60840 Deoxyribonucleic acid, salmon testes 1g

J60437 Fetuin I, fetal bovine serum 100mg250mg

1g

J63276 Fibrinogen, bovine 1g5g

10g25g

J62380 Fibronectin, bovine plasma, sterile-filtered

5mg

J63838 Hemoglobin, bovine 5g25g

100g

J60566 Hyaluronic acid, bovine vitreous humor 10mg50mg

100mg

J61698 Intrinsic factor 1kilounit

J63215 Lactalbumin hydrolysate 500g1kg

2.5kg

J60576 Lecithin, 60%, egg 100g250g500g

J63859 Mucin, bovine submaxillary gland 100mg500mg

J63383 Thrombin, bovine plasma 100kilounits500kilounits

J61046 Transferrin (Holo), bovine plasma, 98+% 1g

J63969 Wheat Germ Oil 5kg10kg15kg

J60287 Yeast Extract 500g1kg


Cytoskeletal Protein Antibodies

Anti-CytokeratinAnti-Cytokeratin antibody is prepared in rabbits with pre-keratins (Citric Acid Soluble Keratins) isolated from bovine tissue. The resulting Cytokeratin antibody is a broad spectrum antibody which will react with both acidic and basic forms of Class I and Class II Keratins (demonstrated by Western immunoblots of human keratinocyte homogenates). The antibody is provided as a whole serum and is excellent for the immunohistochemical staining of both tissues and cultured cells.

Stock No. Name Size

J65649 Anti-Cytokeratin antibody, from rabbit, whole serum

1ml

Anti-GFAP, PolyclonalAnti-GFAP, Polyclonal is prepared in rabbits with homogeneous GFAP isolated from bovine spinal cords. The antibody is essentially monospecific. It stains only the astrocytes in formalin-fixed, paraffin-embedded rat brain cerebellum. Western immunoblots of adult rat spinal cord/cytoskeletal proteins confirmed the monospecificity. Anti-GFAP polyclonal reacts with GFAP containing specimens from human, bovine, rat, dog, cat and mouse.

Stock No. Name Size

J64334 Anti-GFAP antibody, from rabbit 0.5ml

Anti-Myosin, Heavy ChainAnti-Myosin Antibody is prepared in rabbits from bovine uterus myosin. This antisera will react with only heavy chain myosins from smooth muscle and non-muscle sources. Western blot analysis shows that skeletal muscle and cardiac muscle myosins do not cross-react with this antibody. A complete protocol is included with details on immunofluorescent procedures.

Stock No. Name Size

J64913 Anti-Myosin Heavy Chain antibody, from rabbit

0.5ml

Anti-Myosin, Non-Muscle, Heavy ChainNon-Muscle Myosin Antibody is prepared in rabbits from Human Platelet Myosin (>95% Pure via PAGE). The non-Muscle Myosin IgG is then purified from rabbit immune serum. The resulting IgG will bind only heavy chain, non-muscle Myosin in vertebrates. It contains no detectable cross-reactivity with any other protein including skeletal, smooth muscle and cardiac myosins. The antibody can be used for ELISA assays, immunoprecipitations and Western blots of non-muscle Myosin. It can also be used for the immunohistochemical localiztion of non-muscle Myosin in vivo (envisioning the blood vasculature system) and in vitro (discriminating between endothelial and smooth muscle cells). A complete protocol is included with details on immunofluorescent procedures.

Stock No. Name Size

J65418 Anti-Myosin IIA (non-muscle) Heavy Chain antibody, from rabbit

0.5ml

Anti-Myosin, Smooth Muscle, Heavy ChainAnti-Smooth Muscle Myosin is prepared in rabbits from bovine tracheal smooth muscle myosin (>98% Pure via PAGE). The anti-Smooth Muscle Myosin IgG is then purified from rabbit immune serum. The resulting IgG binds predominantly heavy chain smooth muscle myosin in vertebrates. It contains no detectable cross-reactivity with skeletal or non-muscle myosin. The antibody can be used for immunohistochemical localization, Western blots and for immunoprecipitation studies of smooth muscle myosin. A complete protocol is included with details on immunofluorescent procedures.

Stock No. Name Size

J64817 Anti-Myosin (Smooth Muscle) Heavy Chain antibody, from rabbit

0.5ml

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Lipoprotein Products

Human Lipoprotein & Acetylated LDLHuman Low Density Lipoprotein is isolated from blood bank produced human plasma. It is purified via ultracentrifugation to homogeneity determined by agarose gel electrophoresis. Each lot is evaluated for receptor binding to human skin fibroblasts.

Acetylated Human Low Density Lipoprotein, Ac-LDL, is also available. Ac-LDL is purified to homogeneity as demonstrated on agarose gel electrophoresis. Each lot is evaluated for receptor binding to murine peritoneal macrophages.

Stock No. Name Size

J65039 Lipoprotein, low density, human plasma, 99% 5mg

J65029 Lipoprotein, low density, acetylated, human plasma, 99%

2mg

Shelf life for these products is 3 months.

Carbamylated LDL, c-LDLCarbamylated Human LDL is made with Potassium cyanate. Each lot is analyzed for the degree of carbamylation (via diacetylmonoxime assay and the e-carbamyl-Lysine present versus native LDL in nanomoles/mg protein) and the c-LDL migration versus the native LDLon agarose gel electrophoresis.

Stock No. Name Size

J64426 Lipoprotein, low density, carbamylated, human plasma, 99%

1mg


Dil-Lipoprotein, Cell MarkerDil-LDL, Low Density Lipoprotein, labeled with 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate is used to visualize LDL binding sites in cultured cells or to screen for cell mutants defective in the expression of LDL receptors. It may also be useful in evaluating receptor levels by fluorescent activated cell sorting, and examining tissue levels of receptor in vivo. Dil-LDL is an excellent marker of cellular endocytosis. A complete protocol is included.

Stock No. Name Size

J65330 DiI-Lipoprotein, low density, human plasma

200µg

J64267 DiO-Lipoprotein, low density, human plasma

200µg


DiI AcLDL, Cell Marker

• Labels Endothelial Cells & Microphages• Dil Labeled Cells can be Trypsinized• Does Not Label Matrix Associated Proteins• Dil Labeled Cells Remain Viable• Directly Applicable to Cell Sorting & Fluorescence

Microscopy

Acetylated Dil-LipoproteinAcetylated Low Density Lipoprotein, labeled with 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate, labels both vascular endothelial cells and macrophages. It can be used to identify and/or isolate these cells from mixed cell populations. When cells are labeled with Dil-Ac-LDL, the lipoprotein is degraded by lysosomal enzymes and the Dil (fluorescent probe) accumulates in the intracellular membranes. Labeling cells with Dil-Ac-LDL has no effect on cell viability. Pure cultures of vascular endothelial cells can be isolated from complex primary cultures using fluorescent activated cell sorting based on their increased metabolism of the Dil-Ac-LDL. Contaminating cell types (fibroblasts, smooth muscle, pericytes, epithelial cells) are not labeled. Macrophages can be differentiated from mixed cell populations (including endothelial cells) because they are more brightly labeled.

Labeling endothelial cells with Dil-Ac-LDL has many advantages over labeling other endothelial cell associated antigens. The labeling procedure is one step, and once the cells are labeled, the fluorescent probe (Dil) is not removed by Trypsin. Both low density and confluent cultures of vascular endothelial cells are effectively labeled. No other cell type (other than macrophages) is labeled to the same level as vascular endothelial cells. Each lot of Dil-Ac-LDL is evaluated for the specific labeling of bovine aortic endothelial cells and murine macrophages to assure consistent results.

Stock No. Name Size

J65597 DiI-Lipoprotein, low density, acetylated, human plasma

200µg

J64029 DiO-Lipoprotein, low density, acetylated, human plasma

200µg

Includes labeling protocol. Refrigerate at 4°C; do not freeze. Shelf life for these products is 3 months.


Lipoprotein ReagentsHuman Ox-LDLOxidized Human LDL is made via copper sulfate oxidation. Each lot is analyzed for the degree of oxidation and is evaluated ultimately for receptor binding to macrophages.

Stock No. Name Size

J65591 Lipoprotein, low density, oxidized, human plasma, 99%

2mg

J64223 Lipoprotein, low density, oxidized, human plasma, Low-TBAR, 99%

3mg

J65261 Lipoprotein, low density, oxidized, human plasma, Hi-TBAR, 99%

2mg

J64164 DiI-Lipoprotein, low density, oxidized, human plasma

200µg

Refrigerate at 4°C. Shelf life for these products is 6 weeks.

Human VLDLHuman Very Low Density Lipoprotein, VLDL, is isolated from blood bank produced human plasma. It is purified via ultracentrifugation (1.006g/cc) to homogeneity determined on agarose gel electrophoresis.

Stock No. Name Size

J65642 Lipoprotein, very low density, human plasma, 99%

1mg

J65568 DiI-Lipoprotein, very low density, human plasma

200µg 1mg

Membrane filtered and aseptically packaged. Refrigerate at 4°C. Shelf life for these products is 3 months.

Human HDLHuman High Density Lipoprotein (HDL) is isolated from blood bank produced human plasma. It is purified via ultracentrifugation (1.063-1.21g/cc) to homogeneity determined on agarose gel electrophoresis.

Stock No. Name Size

J64903 Lipoprotein, high density, human plasma, 99%

10mg

J65503 DiI-Lipoprotein, high density, human plasma

200µg 1mg

Aseptically packaged. Refrigerate at 4°C. Shelf life for these products is 3 months.

Lipoprotein Deficient

SerumLipoprotein Deficient Serum (LPDS) is prepared by ultracentrifugation and is extensively dialyzed. Each lot is evaluated in conjunction with LDL to determine the degree of up regulation of LDL receptors.

Stock No. Name Size

J65182 Lipoprotein Deficient Serum, bovine 2x25ml

J65516 Lipoprotein Deficient Serum, human 25ml

Store frozen at @-20°C. Shelf life for these products is 6-12 months.

ApolipoproteinsApolipoproteins; Apo A-I and Apo A-II are both isolated from our Human HDL. They are purified via column chromatography (>95% PAGE).

Stock No. Name Size

J64506 Apolipoprotein A-I, human plasma 95% 1mg

J64658 Apolipoprotein A-II, human plasma, 95% 1mg

J64606 Lipoprotein(a), human plasma, 99% 100µg

Membrane filtered (0.22 micron) and aseptically packaged in aqueous solution. J64506 and J64658, Store frozen @-20ºC, shelf life is 6 months. J64606 Refrigerate at 4°C, shelf life is 6 weeks.

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anti-Human LDL Anti-Human LDL is prepared in rabbits using purified LDL immunogen. The resulting serum is affinity purified on an LDL sepharose column. Both human LDL and VLDL react with the antibody but not human HDL as determined via immunodiffusion and ELISA methods. The antibody is suitable for the immunohistochemical localization of LDL, Ac-LDL and Ox-LDL in cells or tissues. Each lot is analyzed for the localization of LDL or Ac-LDL receptors by ligand blotting proedures.

Stock No. Name Size

J64398 Anti-Human LDL antibody, from rabbit 1mg


CalmodulinCalmodulin is purified to >99.5% as demonstrated by silver stained, two dimensional polyacrylamide gel electrophoresis. The biological activity is 48,000 units/mg (one unit of Calmodulin gives a 50% activation of 0.14 units of bovine retina adenylate cyclase where one unit of adenylate cyclase is equivalent to the production of one nanomole of Cyclic AMP per minute per milligram of protein at 37°C). Suitable for radioiodination and as a standard in biological and immunohistochemical experiments.

Stock No. Name Size

J60231 Calmodulin, bovine testes 1mg2.5mg

Anti-Tamm-Horsfall GlycoproteinAnti-Tamm-Horsfall Glycoprotein is prepared in rabbits using chromatography pure THG. No significant cross-reactivity with urinary or other related proteins was detected. This antibody is suitable for ELISA assays (1:150,000 against solid phase antigen) and localization studies on cells and tissues (1:100 to 1:250).

Stock No. Name Size

J65429 Anti-Tamm Horsfall Glycoprotein antibody, from rabbit

0.5ml

References1. Lynley AM, Dale BA, Biochem Biophys Acta 744: 28-35 (1983).2. Fleckman P, Dale BA, Holbrook KA. J. Invest Dermatol 85: 5-7-512 (1985).3. Sybert VP, Dale BA, Holbrook KA. J. Invest Dermatol 84: 191-194 (1985).4. Dale BA, Gown AM, Fleckman P. Kimball JR, Resing KA. J. Invest Dermatol 88: 306-313 (1987).5. Kanitakis J, Ramirez-Bosca A, Reno A, Viac J, Roche P. Thivolet J. Virchows Archiv A. Pathol Anat Histopathol 412: 375-382 (1988).6. Phillps, M.A; Stewart, B.E; Qin, Q; Chakravarty, R;Floyd, E; Jetten, A.m; and Rice, R.H. (199) Primary Structure of Keratinocyte Transglutaminase. Proc. Natl. Acad. Sci. 87: 9333-9337.7. Schmidt, R; Michel, S; Shroot, B; and U. Reichert. (1988) Transglutaminase in normal and transformed human keratinocytes in culture, J. Invest. Dermatol. 90: 475-479.8. Michel, S; Courseaux, A; Miguel C; Bernardon, J.M; Schmidt, R; Shroot, B; Thacher, S.M; and U. Reichert (1991) Determination of retinoid activity by an enzyme-linked immunosorbent assay. Anal. Biochem. 192: 232-236.9. Huber, M; Rettler, I; Bernasconi, K; Frenk, E; Lavrijsen, S; Ponec,M; Bon, A; Lautenschlager, D; Schorderet, D; and Hohl, D. (1995) Mutations of Keratinocyte Transglutaminase in Lamellar Ichthyosis. Science. 267: 525-527.10. Murphy, G.F., Flynn, T.C., Rice, R.H. and Pinkus, G.S. (1984) Involucrin expression in normal and neplastic human skin: a marker for keratinocyte differentiation. J. Invest Dermatol. 82: 453-457.11. Rice, R.H., Pinkus, G.S., Warhol, M.J. and Antoniolo, D.A. (1984) Involucrin: biochemistry and immunohistochemistry in: Advances in immunohistochemistry (S. Sternberg and R. DeLellis, eds.), Masson Publishing, USA pp. 111-125.12. Said, J.W., Nash, G., Sassoon, A.F., Shintaku, I.P., and Banks-Schlegel, S. (1984) Involucrin in lung tumors. A specific marker for squamous differentiation. Lab Invest. 49: 563-568.13. Said, J.W., Sassoon, A.F., Shintaku, I.P., and Banks-Schlegel, S. (1984) Involucrin in Squamous and Basal Cell Carcinomas of the Skin: An Immunohistochemical study. J. Invest. Dermatol. 82: 449-452.14. Warhol, M.J., Antonioli, D.A., Pinkus, G.S., Burke, L. and Rice, R.H. (1982) Immunoperoxidase staining for involucrin, a potential diagnostic aid in cervicovaginal pathology. Human Pathol. 13: 1095-1099.15. Warhol, N.M., Pinkus, G.S, Rice, R.H., El-Tawil, G.H., Lancaster, W.D., Jenson, A.B. adn Kurman, R.J. (1984) Papillomavirus infection of the cervix. III: Relationship of the presence of viral structural proteins to the expression of involucrin. Int. J. Gynecol. Pathol. 3: 71-81.16. Warhol. M.J., Rice, R.H., Pinkus, G.S. and Robboy, S.J. (1984) Evaluation of squamous epithelium in adenocanthoma and adenosquamous carcinoma of the endometrium: Immunoperoxidase analysis of involucrin and keratin localization. Int. J. Gynecol. Pathol 3: 82-91.17. Kaplan, M.J., Mill, S.E., Rice, R.H., and Johns, M.E. (1984) Involucrin in laryngeal dysplasia. A marker for differentiation. Arch. of Otolaryngology. 110: 713-716.18. Walts, A.E., Said, J.W., Siegel, M.B. and Banks-Schlegel, S. (1985) Involucrin, a marker of squamous and urothelial differentiation. An immunohistochemical stufy on its distribution in normal and neoplastic tissues. J. of Pathology 145: 329-340.19. Sassoon, A.F., Said, J.W., Nash, G.N, Shintaku, I.P. and Banks-Schlegel, S. (1985) Involucrin in intraepithelial and invasive squamous cell carcinomas of the cervix: An immunohistochemical study. Human Pathology 16: 467-470.20. Rice, R.H. and Thacher, S.M. (1986) Involucrin: A constituent of cross-linked envelopes and marker of squamous maturation. In: biology of the integment Vol. 2 Vertebrates (J. Bereiter-Hahn, A.G. Matoitsy, K.S. Richards eds.) Springer-Verlag. Berlin Heidelberg, Germany. 752-761.21. Smoller, B.R., Kwan, T.H., Said, J.W., and Banks-Schlegel, S. (1986) Keratoacanthoma and squamous cell carcinoma of the skin: Immunohistochemical localization of involucrin and keratin proteins. J. Am. Acad. Dermatology 14: 226-234.22. Michael, S., Schmidt, R., Robinson, S.M., Schroot, B., and Reichert, U. (1987) Identification and subcellular distribution of cornified envelope precursor proteins in the transformed human keratinocyte line KV-K14. J. Invest. Dermatol. 88: 301-305.23. Mansbridge, J.N. and Knapp, A.M. (1987) Changes in keratinocyte maturation during wound healing. J. Invest. Dermatol. 89: 253-263.24. Greenberg, A.M. (1987) Involucrin expression as a marker for neoplastic changes in oral mucosa. J. of Oral & Maxillofacial Surgery 45. No. 11 (Abstract).25. Goldstein, J.L., Y.K., Ho. S.K. Basu, and M.S. Brown. (1979) Binding site on macrophages that mediates uptake degradation of actylated low density lipoprotein, producing massive cholesterol deposition. Proc. Nat. Acad Sci. USA 76: 335-337.26. Folgelman, A.M., I. Schechter, J. Seager, M. Hokum, J.S. Child and P.A. Edwards. (1980) Malondialdehyde alteration of low density lipoproteins leads to cholesterylester accumulation in human monocyte-macrophages. Proc. Nat. Acad. Sci. 77: 2214-2218.27. Stein, O. and Y. Stein. (1980) Bovine aortic endothelial cells display macrophage-like properties toward acetylated [I125]-labeled low density lipoprotein. Biochem. Biophys. Acta 620: 631-635.28. Pitas, R.E., T.L. innerarity, J.N. Weinstein, and R.W. Mahley. (1981) Acetoacetylated lipoproteins used to distinguish fibroblasts from macrophages in vitro by fluorescence microscopy. Arteriosclerosis 1: 177-185.29. Voyta, J.C., D.P. Via, C.E. Butterfield and B.R. Zetter. (1984) Identification and isolation of endothelial cells based on thei increased uptake of acetylated-low density lipoprotein. J. Cell Biology. 99: 2034-2040.30. Giulian, D. and D.G. Young. (1986) Brain peptides and glial growth. II. Identification of cells that secrete glia-promoting factors. J. Cell Biology. 102: 812-820.31. Netland, P.A., B.R. Zetter, D.P. Via and J.C. Voyta. (1985) Insitu labeling of vascular endothelium with fluorescent acetylated low density lipoprotein. Histochemical Journal. 17: 1309-1320.32. Pitas, R.E., J. Boyles, R.W. Mahley and D.M. Bissell (1985) Uptake of chemically modified low density lipoproteins in vivo is mediated by specific endothelial cells. J. Cell Biology. 100: 103-117.33. Giulian, D., and T.J. Baker (1986) Characterization of ameboid microglia isolated from developing mammalian brain. J. Neuroscience. 6: 2163-2178.34. Bjorling, D.E., R. Saban, W.M. Tengowski, S.M. Gruel and V.K. Rao. (1992) Removal of Venous Endothelium with Air. J. Pharm. & Tox. Methods. 28: 149-157.35. Pitas, R.E., et al. Arteriosclerosis 1: 177 (1983).36. Pitas, R.E., et al. Arteriosclerosis 3: 1 (1983).37. Barak, L.S., Webb, W.W. J.Cell Biology 90: 595 (1981).38. Barak, L.S., Webb, W.W. J.Cell Biology 95: 846 (1982).39. Kingsley, D.M., Kreiger, M. PNAS, USA, 81: 454 (1984).40. Voyta, J.C., et al. J. Cell Biology 99: 2034 (1984).41. Kreiger, M., et al. J. Receptor Res. 3: 361 (1983).42. Pitas, R.E., et al. J. Cell Biology 100: 103 (1985).43. Herman, B., Albertini, D.F., J. Cell Biology 98: 565 (1984).44. Grove, R.I. et al. J. Lipid Res. 32: 1889-1897 (1991).45. Hamaguchi Y., Iwasa F., Biomedical Research. 1: 502-509 (1980).46. Pardue, R.L., et al, Cell, 23: 533-542 (1981).47. Zavortink, M., et al, Exp. Cell Res. 149: 375-385 (1983).48. Luby, Phelps K., et al, JBC. 101: 1245-1256 (1985).49. I. Tamm and F.L. Horsfall, Proc. Soc. Exp. Biol. Med. 74: 108-114 (1950).50. J.R. Hoyer, and M.W. Seiler, Kidney Int. 16: 279-289 (1979).51. Janssens, P.M.J. et al. Clinical Chemistry 38: 216-222 (1992).

Biochemical Products

Alfa Aesar offers a wide range of biochemical products for a variety of applications. Please visit www.alfa.com/bio for a full listing of available products.

Antibiotics

Stock No. Name Size

J60148 Actinomycin D 5mg

J61491 Amphotericin B 100mg1g5g

J62432 Bacitracin 5g25g

J62671 G418 disulfate, Cell Culture Reagent 1g5g

25g

J63871 G418 disulfate, 50mg/ml solution 10ml50ml

J62834 Gentamycin sulfate, 600 I.U./mg 1g5g

10g

J60668 Kanamycin monosulfate, Cell Culture Grade 1g5g

10g

J61669 Monensin sodium salt 1g5g

J61499 Neomycin sulfate hydrate 25g100g

J62486 Nystatin 5g10g

J62489 Oxytetracycline hydrochloride 25g100g

J63074 Polymixin B sulfate 1g5g

J61278 Puromycin dihydrochloride, 99+% 25mg100mg500mg

J61820 Spectinomycin dihydrochloride pentahydrate, Cell Culture Grade

5g25g

J61299 Streptomycin sulfate, Cell Culture Reagent 50g100g

J61601 Streptozotocin 500mg1g

J61714 Tetracycline 5g25g

100g

J62217 Tunicamycin 10mg

Apoptosis Reagents

Stock No. Name Size

J62340 Brefeldin A, 99% 10mg25mg50mg

J60342 Clofibrate 1g5g

25g100g

J61072 Colchicine 500mg1g5g

J63191 Cyclosporin A, 99+% 1g5g

J63190 Ebselen 5mg10mg

J61688 Haloperidol 5g25g

J63193 Mitomycin C 5mg10mg

J63509 Tamoxifen, 98% 500mg1g5g

J60955 Tamoxifen Citrate 1g5g

J63598 Vinblastine sulfate 10mg50mg

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Buffers

Stock No. Name Size

J63666 Citrate-buffered saline (20X) 1L2L

J63115 Enzyme storage buffer in PBS and glycerol, autoclaved

125ml250ml

J63264 Enzyme storage buffer in TBS and glycerol, autoclaved

125ml250ml

J60262 FSB buffer 125ml250ml

J63719 Kinase Storage Buffer 100ml250ml

J63892 Lysis buffer, pH 8.0 500ml

J62036 Phosphate-buffered saline (PBS, 10X), pH 7.4 1L2L4L

J60801 Phosphate-buffered saline (PBS, 10X), pH 7.4, for Western Blot

1L2L4L

J62851 Phosphate-buffered saline (PBS, 10X), RNase free

250ml500ml

J61196 Phosphate-buffered saline (PBS, 1X), Sterile 500ml

J62990 RBC lysis buffer for human cells 250ml500ml

J62150 RBC lysis buffer for mouse cells 250ml500ml

J63037 Transfer or electro blotting buffer (10X), pH 8.5

1L2L4L

J62318 TSS (Transformation & Storage Solution), pH 6.5

125ml

J61459 Yeast lysis solution for DNA isolation 250ml500ml

Protease Inhibitors

Stock No. Name Size

H26473 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, 97%

250mg1g

J60011 Antipain 50mg

J63039 Aprotinin, from bovine lung 10mg25mg

100mg

J61106 Bestatin 100mg250mg

J62933 E-64 5mg25mg

J63241 4’,5,7-Trihydroxyisoflavone, 99+% 100mg1g

10g

J63445 Genistin, 99+% 25mg100mg

J61188 Leupeptin hemisulfate 25mg100mg

J60237 Pepstatin A, 98% 5mg25mg

J61852 Protease Inhibitor Cocktail, for general use 1ml

Stains, Dyes & Indicators

Stock No. Name Size

J60048 Acriflavine hydrochloride 10g25g

J60972 3,3'-Diaminobenzadine, tablets 50ea100ea

J62216 3,3'-Diaminobenzadine tetrahydrochlo-ride hydrate

5g10g

J62619 Erythrosin B, spirit soluble 5g25g

100g

J63388 Fluo 3-AM 1mg

J61549 Fluorescien disodium salt 100g500g

J62728 FURA 2-AM 1mg

J62475 7-Methoxycoumarin-4-acetic acid, 99% 100mg250mg

1g

J60230 Nitro Blue Tetrazolium chloride, 99% 1g5g

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biomedical research products · present in blood plasma. fibronectins are used to facilitate the...

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