biomarkers of anorexia nervosa - intact-rtn.eu · anorexia nervosa refusal /failure to maintain a...
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Anorexia Nervosa
� Refusal /failure to maintain a normal
body weight
� Highest mortality among psychiatric
disorders
� Heritability estimates 50 – 80%
[Kaye, Physiol Behav, 2008]
[Gorwood et al, Eur J Pharmacol, 2003]
Proteome-wide analysis
� Hypothesis generating approach
� Identify biomarkers
� Research: New candidates in aetiology and course of disease of Anorexia
� Clinical: Monitor disease severity and predict onset, relapse or outcome
� No studies on eating disorders yet
[Jacobs et al; J Prot Res; 2005]
[Schwarz and Bahn; Electrophoresis; 2008]
Study population
Matched on age, sex and ethnicity
� 11 current AN patients
� 8 recovered AN patients
� 17 controls
80% Power requires 20 – 30
per group
Overview sample BMI
0
5
10
15
20
25
Expectations
State
Trait
Biomarker?
Causality?
AN recov. controls
Methods
� Blood plasma
� No food two hours prior to blood collection
� Centrifugal speed 3000g
� Store at -80○ Celsius within 2 hours
� 2D-gel
� Charge (Iso-electric focussing pH 3 – 11)�
� Mass (Polyacrylamide gel electrophoresis)�
� Analysis with Progenesis SameSpots software
[Hye et al, Brain, 2006]
Methods continued
Mass
Charge (pH) �
� Comparing the relative amount
of each protein
Methods continued
Protein selection – 1
Protein selection – 2
Next steps
� Increase sample size
� Identify main spots by mass spectrometry
� Validation and replication
� Longitudinal case-only study
� Process of change in AN patients
� Eating Disorders Department
� Therapists and phlebotomists
Maudsley Hospital
� MRC SGDP centre
� Lovestone group
Acknowledgements
� Ricardo Sainz-Fuertes *
� Frederique van den Eynde
� Anna Oldershaw
� Amy Harrison
� David Collier *
� Ulrike Schmidt
� Iain Campbell
� Janet Treasure
Marie Curie Actions
� INTACT Research
Training Network
� http://www.intact-rtn.eu