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© 2014 Pearson Education, Inc. Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications, and Issues Fifth Edition Judith Goodenough Betty McGuire 21 DNA and Biotechnology

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Page 1: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Lecture Presentation

Anne Gasc

Hawaii Pacific University and

University of Hawaii–Honolulu Community College

BIOLOGY OF HUMANSConcepts, Applications, and Issues

Fifth Edition

Judith Goodenough Betty McGuire

21DNA and

Biotechnology

Page 2: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

DNA and Biotechnology

OUTLINE:

Form of DNA

Replication of DNA

Gene Expression

Mutations

Regulating Gene Activity

Genetic Engineering

Genomics

Page 3: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Form of DNA

DNA (deoxyribonucleic acid)

Double-stranded molecule twisted to form a double

helix

Resembles a ladder

Each side and half of each rung is made from a string of

repeating units called nucleotides

Nucleotide

One sugar (deoxyribose in DNA)

One phosphate

One nitrogenous (nitrogen-containing) base

Page 4: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Form of DNA

DNA has four nitrogenous bases

Adenine (A)

Guanine (G)

Thymine (T)

Cytosine (C)

Page 5: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Form of DNA

Rungs of ladder consist of paired nitrogenous bases

attached according to complementary base pairing

rules

Adenine (A) pairs only with thymine (T)

Cytosine (C) pairs only with guanine (G)

Each pair of bases held together by hydrogen bonds

Genetic information is encoded in the exact

sequence of bases

Page 6: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Form of DNA

Web Activity: The Structure of DNA

Page 7: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Figure 21.1 DNA molecule.

Page 8: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Replication of DNA

DNA must be exactly copied (replicated) before both

mitotic and meiotic cell divisions

Begins when an enzyme breaks the hydrogen bonds

that hold together the paired bases of the parent

molecule

Two strands of the double helix separate and unwind

Nitrogenous bases on the separated regions are

temporarily exposed

Free nucleotide bases in the nucleus attach to exposed

complementary bases

Page 9: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Replication of DNA

DNA polymerases link the sugars and phosphates

As each of the new double-stranded DNA molecules

forms, it twists into a double helix

DNA replication is described as semiconservative

In each of the new double-stranded:

One original (parent) strand is saved (conserved)

Other (daughter) strand is new

Complementary base pairing creates two new DNA

molecules that are identical to the parent molecule

Genetic information is passed accurately from a parent cell

to daughter cells

Page 10: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Figure 21.2 DNA replication is called semiconservative.

Page 11: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Gene Expression

How does DNA direct cellular activities?

DNA codes for RNA, which codes for proteins

Proteins play structural or functional roles in cells

Gene

Segment of DNA with instructions for producing a

particular protein (or polypeptide)

Expressed when the protein it codes for is produced

Page 12: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

RNA Synthesis

RNA (ribonucleic acid) differs from DNA

Single-stranded

Contains the base uracil (U), and not thymine (T)

Contains the sugar ribose, not deoxyribose

Page 13: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Table 21.1 Comparisons of DNA and RNA

Page 14: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

RNA Synthesis

Occurs in two steps

Transcription

Translation

Transcription

Synthesis of messenger RNA (mRNA) using DNA as

a template

mRNA carries DNA’s instructions for synthesizing a

protein from the nucleus to the cytoplasm

Page 15: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

RNA Synthesis

Steps in transcription

DNA unzips along hydrogen bonds

Free RNA nucleotides pair with the complementary DNA bases

(C-G and U-A) along the exposed DNA strand, forming an RNA

transcript

RNA transcript released from the DNA

DNA closes again

Promoter: DNA sequence that starts transcription

RNA polymerase: enzyme that binds with promoter, opens up

DNA helix, and aligns and links together the RNA nucleotides

Another sequence of bases is the stop signal

Page 16: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Figure 21.3 Transcription.

Page 17: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

RNA Synthesis

Modifications to mRNA before it leaves the nucleus

Removal of introns

Introns intervening sequences

Regions of DNA between promoter and stop signal that

do not have codes that will be translated into a protein

Regions of mRNA corresponding to introns are snipped

out by enzymes

Exons expressed sequences

Left to direct protein synthesis

Page 18: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

RNA Synthesis

Web Activity: Transcription

Page 19: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Figure 21.4 Newly formed messenger RNA.

Page 20: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Table 21.2 Review of the Functions of RNA

Page 21: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Protein Synthesis

Translation

Converts the nucleotide language of mRNA into the amino

acid language of a protein

Genetic code

Converts the DNA base sequence into an amino acid

sequence

Codon

“Words” in the genetic code

A three-base sequence that translates into one amino acid

Example: UUC on mRNA specifies the amino acid

phenylalanine

Page 22: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Protein Synthesis

Stop codons

UAA, UAG, and UGA

Signal the end of a protein

Do not code for an amino acid

Page 23: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Protein Synthesis

Web Activity: The Genetic Code

Page 24: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Figure 21.5 The genetic code.

Page 25: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Transfer RNA (tRNA)

Converts genetic message carried by mRNA into

particular sequence of amino aids

Recognizes both the codon on mRNA and the amino

acid specified by the codon

Enzymes ensure tRNA binds with correct amino acid

Ferries amino acid to the correct location along the

mRNA strand

Page 26: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Protein Synthesis

Anticodon

A three-base sequence on tRNA that is

complementary to the mRNA codon

Binds to codon on mRNA and specific amino acid is

added to growing polypeptide chain

Page 27: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Figure 21.6 A tRNA molecule is a short strand of RNA.

Page 28: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Protein Synthesis

Ribosomes

Structure

Two subunits, each composed of ribosomal RNA (rRNA)

and protein

Function

Bring tRNA bearing an amino acid close enough to

mRNA to interact

Permit alignment of anticodon and codon

Page 29: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Figure 21.7 A ribosome.

Page 30: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Protein Synthesis

Three stages of translation

1. Initiation

Major players in protein synthesis come together

(mRNA, tRNA, and ribosomal subunits)

Page 31: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Figure 21.8 Initiation of translation.

Page 32: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Protein Synthesis

2.Elongation

Occurs as enzymes in the ribosome link the amino

acids, forming a growing chain of amino acids

Polysome

Cluster of ribosomes simultaneously translating the

same mRNA

Page 33: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Figure 21.9 Elongation of the peptide during translation.

Page 34: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Figure 21.10 A polysome.

Page 35: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Protein Synthesis

3.Termination

Occurs when a stop codon moves into the ribosome

Page 36: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Protein Synthesis

Web Activity: Translation

Page 37: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Figure 21.11 Termination of translation.

Page 38: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Mutations

Changes in DNA

Passed to offspring only when they occur in a gamete

Types

Chromosomal

Occur when whole sections of chromosomes are duplicated

or deleted

Gene

Changes in the order of nucleotides in DNA

Those involving insertion or deletion of nucleotides can

greatly change resulting protein

Page 39: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Figure 21.12 A base-pair substitution is a DNA mutation.

Page 40: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Regulating Gene Activity

Only certain genes are active in a certain type of cell

and most genes are turned off in any given cell

This leads to specialization

Active genes produce specific proteins that determine

the structure and function of a particular cell

Page 41: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Regulating Gene Activity

Gene activity is regulated simultaneously at several

levels

Chromosomal level: coiling and uncoiling of DNA

(when DNA is tightly coiled, genes are not expressed)

Transcription of genes: enhancers increase rate of

transcription of certain genes

Steroid hormones turn on specific genes

Page 42: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Genetic Engineering

Biotechnology

Field in which scientists make controlled use of living

cells to perform specific tasks

Genetic engineering

A subset of biotechnology involving the manipulation

of DNA for human purposes

Some uses

Produce drugs and hormones

Improve treatments of human diseases

Increase food production from plants and animals

Page 43: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Recombinant DNA

DNA made from two or more sources

Created when a gene of interest (one that produces

a useful protein or trait) is put into another piece of

DNA

Page 44: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Recombinant DNA

Web Activity: Recombinant DNA

Page 45: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Recombinant DNA

Steps in creating recombinant DNA

1. Restriction enzymes used to cut gene of interest out of

original DNA and splice it into vector DNA

2. Plasmids are common vectors (circular pieces of DNA that

exist separately from bacterial chromosome)

3. Vector (recombined plasmids) taken up by bacteria (new

host cells)

4. Bacteria with gene of interest identified and isolated

5. Produce numerous copies of the gene (amplify the gene)

Bacterial cloning

Polymerase chain reaction (PCR)

Page 46: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Recombinant DNA

Web Activity: PCR

Page 47: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Figure 21.13 DNA splicing.

Page 48: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Figure 21.14 Overview of genetic engineering using plasmids.

Page 49: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Figure 21.15 Polymerase chain reaction (PCR).

Page 50: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Applications of Genetic Engineering

Environmental—example: oil-eating microbes that can

withstand ocean conditions

Livestock—example: cows that produce more milk

Pharmaceuticals—example: gene pharming

Create transgenic animals that produce a protein with

medicinal value in their milk, eggs, or blood

Collect and purify protein

Raw materials—example: goats and bacteria genetically

engineered to produce spider silk proteins

Agriculture—example: crops genetically engineered to

be resistant to pests

Page 51: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Figure 21.17 Creating a transgenic animal.

Page 52: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Gene Therapy

Put functional genes into body cells affected by

defective (mutant) gene

Viruses used to deliver healthy gene

Potential to cure inherited diseases

Some success with severe combined

immunodeficiency disease (SCID) and X-SCID

(mutant gene on X chromosome)

Page 53: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Figure 21.18 Gene therapy using a virus.

Page 54: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Genomics

Genome

Entire set of genes carried by one member of a

species

Genomics

Study of entire genomes

Page 55: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Genomics

Human Genome Project

Sequenced all of the DNA in a representative human

cell

Human genome consists of 20,000 to 25,000 genes

Humans are identical in 99.9% of the sequences of

their genes

Page 56: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Genomics

Microarray

Tool used to analyze gene activity under different

conditions

Thousands of DNA sequences stamped onto a single

glass slide (DNA chip)

Can be used to identify genes that are active in

cancerous cells, but not in healthy cells

Page 57: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Genomics

Comparisons of genomes of different species

provide evidence of common evolutionary past

We share 50% of our genes with fruit flies

We share 90% of our genes with mice

Page 58: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

Figure 21.19 A comparison of microarrays.

Page 59: Biology of Humans 2/e - Napa Valley...Lecture Presentation Anne Gasc Hawaii Pacific University and University of Hawaii–Honolulu Community College BIOLOGY OF HUMANS Concepts, Applications,

© 2014 Pearson Education, Inc.

You Should Now Be Able To:

Know the form of DNA

Know the process of DNA replication

Know all the steps of gene expression

Understand what are mutations

Understand the regulation of gene activity

Understand genetic engineering and genomics