biology of humans 2/e - napa valley...lecture presentation anne gasc hawaii pacific university and...
TRANSCRIPT
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Lecture Presentation
Anne Gasc
Hawaii Pacific University and
University of Hawaii–Honolulu Community College
BIOLOGY OF HUMANSConcepts, Applications, and Issues
Fifth Edition
Judith Goodenough Betty McGuire
21DNA and
Biotechnology
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DNA and Biotechnology
OUTLINE:
Form of DNA
Replication of DNA
Gene Expression
Mutations
Regulating Gene Activity
Genetic Engineering
Genomics
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Form of DNA
DNA (deoxyribonucleic acid)
Double-stranded molecule twisted to form a double
helix
Resembles a ladder
Each side and half of each rung is made from a string of
repeating units called nucleotides
Nucleotide
One sugar (deoxyribose in DNA)
One phosphate
One nitrogenous (nitrogen-containing) base
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Form of DNA
DNA has four nitrogenous bases
Adenine (A)
Guanine (G)
Thymine (T)
Cytosine (C)
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Form of DNA
Rungs of ladder consist of paired nitrogenous bases
attached according to complementary base pairing
rules
Adenine (A) pairs only with thymine (T)
Cytosine (C) pairs only with guanine (G)
Each pair of bases held together by hydrogen bonds
Genetic information is encoded in the exact
sequence of bases
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Form of DNA
Web Activity: The Structure of DNA
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Figure 21.1 DNA molecule.
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Replication of DNA
DNA must be exactly copied (replicated) before both
mitotic and meiotic cell divisions
Begins when an enzyme breaks the hydrogen bonds
that hold together the paired bases of the parent
molecule
Two strands of the double helix separate and unwind
Nitrogenous bases on the separated regions are
temporarily exposed
Free nucleotide bases in the nucleus attach to exposed
complementary bases
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Replication of DNA
DNA polymerases link the sugars and phosphates
As each of the new double-stranded DNA molecules
forms, it twists into a double helix
DNA replication is described as semiconservative
In each of the new double-stranded:
One original (parent) strand is saved (conserved)
Other (daughter) strand is new
Complementary base pairing creates two new DNA
molecules that are identical to the parent molecule
Genetic information is passed accurately from a parent cell
to daughter cells
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Figure 21.2 DNA replication is called semiconservative.
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Gene Expression
How does DNA direct cellular activities?
DNA codes for RNA, which codes for proteins
Proteins play structural or functional roles in cells
Gene
Segment of DNA with instructions for producing a
particular protein (or polypeptide)
Expressed when the protein it codes for is produced
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RNA Synthesis
RNA (ribonucleic acid) differs from DNA
Single-stranded
Contains the base uracil (U), and not thymine (T)
Contains the sugar ribose, not deoxyribose
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Table 21.1 Comparisons of DNA and RNA
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RNA Synthesis
Occurs in two steps
Transcription
Translation
Transcription
Synthesis of messenger RNA (mRNA) using DNA as
a template
mRNA carries DNA’s instructions for synthesizing a
protein from the nucleus to the cytoplasm
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RNA Synthesis
Steps in transcription
DNA unzips along hydrogen bonds
Free RNA nucleotides pair with the complementary DNA bases
(C-G and U-A) along the exposed DNA strand, forming an RNA
transcript
RNA transcript released from the DNA
DNA closes again
Promoter: DNA sequence that starts transcription
RNA polymerase: enzyme that binds with promoter, opens up
DNA helix, and aligns and links together the RNA nucleotides
Another sequence of bases is the stop signal
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Figure 21.3 Transcription.
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RNA Synthesis
Modifications to mRNA before it leaves the nucleus
Removal of introns
Introns intervening sequences
Regions of DNA between promoter and stop signal that
do not have codes that will be translated into a protein
Regions of mRNA corresponding to introns are snipped
out by enzymes
Exons expressed sequences
Left to direct protein synthesis
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RNA Synthesis
Web Activity: Transcription
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Figure 21.4 Newly formed messenger RNA.
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Table 21.2 Review of the Functions of RNA
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Protein Synthesis
Translation
Converts the nucleotide language of mRNA into the amino
acid language of a protein
Genetic code
Converts the DNA base sequence into an amino acid
sequence
Codon
“Words” in the genetic code
A three-base sequence that translates into one amino acid
Example: UUC on mRNA specifies the amino acid
phenylalanine
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Protein Synthesis
Stop codons
UAA, UAG, and UGA
Signal the end of a protein
Do not code for an amino acid
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Protein Synthesis
Web Activity: The Genetic Code
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Figure 21.5 The genetic code.
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Transfer RNA (tRNA)
Converts genetic message carried by mRNA into
particular sequence of amino aids
Recognizes both the codon on mRNA and the amino
acid specified by the codon
Enzymes ensure tRNA binds with correct amino acid
Ferries amino acid to the correct location along the
mRNA strand
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Protein Synthesis
Anticodon
A three-base sequence on tRNA that is
complementary to the mRNA codon
Binds to codon on mRNA and specific amino acid is
added to growing polypeptide chain
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Figure 21.6 A tRNA molecule is a short strand of RNA.
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Protein Synthesis
Ribosomes
Structure
Two subunits, each composed of ribosomal RNA (rRNA)
and protein
Function
Bring tRNA bearing an amino acid close enough to
mRNA to interact
Permit alignment of anticodon and codon
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Figure 21.7 A ribosome.
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Protein Synthesis
Three stages of translation
1. Initiation
Major players in protein synthesis come together
(mRNA, tRNA, and ribosomal subunits)
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Figure 21.8 Initiation of translation.
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Protein Synthesis
2.Elongation
Occurs as enzymes in the ribosome link the amino
acids, forming a growing chain of amino acids
Polysome
Cluster of ribosomes simultaneously translating the
same mRNA
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Figure 21.9 Elongation of the peptide during translation.
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Figure 21.10 A polysome.
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Protein Synthesis
3.Termination
Occurs when a stop codon moves into the ribosome
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Protein Synthesis
Web Activity: Translation
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Figure 21.11 Termination of translation.
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Mutations
Changes in DNA
Passed to offspring only when they occur in a gamete
Types
Chromosomal
Occur when whole sections of chromosomes are duplicated
or deleted
Gene
Changes in the order of nucleotides in DNA
Those involving insertion or deletion of nucleotides can
greatly change resulting protein
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Figure 21.12 A base-pair substitution is a DNA mutation.
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Regulating Gene Activity
Only certain genes are active in a certain type of cell
and most genes are turned off in any given cell
This leads to specialization
Active genes produce specific proteins that determine
the structure and function of a particular cell
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Regulating Gene Activity
Gene activity is regulated simultaneously at several
levels
Chromosomal level: coiling and uncoiling of DNA
(when DNA is tightly coiled, genes are not expressed)
Transcription of genes: enhancers increase rate of
transcription of certain genes
Steroid hormones turn on specific genes
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Genetic Engineering
Biotechnology
Field in which scientists make controlled use of living
cells to perform specific tasks
Genetic engineering
A subset of biotechnology involving the manipulation
of DNA for human purposes
Some uses
Produce drugs and hormones
Improve treatments of human diseases
Increase food production from plants and animals
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Recombinant DNA
DNA made from two or more sources
Created when a gene of interest (one that produces
a useful protein or trait) is put into another piece of
DNA
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Recombinant DNA
Web Activity: Recombinant DNA
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Recombinant DNA
Steps in creating recombinant DNA
1. Restriction enzymes used to cut gene of interest out of
original DNA and splice it into vector DNA
2. Plasmids are common vectors (circular pieces of DNA that
exist separately from bacterial chromosome)
3. Vector (recombined plasmids) taken up by bacteria (new
host cells)
4. Bacteria with gene of interest identified and isolated
5. Produce numerous copies of the gene (amplify the gene)
Bacterial cloning
Polymerase chain reaction (PCR)
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Recombinant DNA
Web Activity: PCR
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Figure 21.13 DNA splicing.
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Figure 21.14 Overview of genetic engineering using plasmids.
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Figure 21.15 Polymerase chain reaction (PCR).
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Applications of Genetic Engineering
Environmental—example: oil-eating microbes that can
withstand ocean conditions
Livestock—example: cows that produce more milk
Pharmaceuticals—example: gene pharming
Create transgenic animals that produce a protein with
medicinal value in their milk, eggs, or blood
Collect and purify protein
Raw materials—example: goats and bacteria genetically
engineered to produce spider silk proteins
Agriculture—example: crops genetically engineered to
be resistant to pests
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Figure 21.17 Creating a transgenic animal.
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Gene Therapy
Put functional genes into body cells affected by
defective (mutant) gene
Viruses used to deliver healthy gene
Potential to cure inherited diseases
Some success with severe combined
immunodeficiency disease (SCID) and X-SCID
(mutant gene on X chromosome)
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Figure 21.18 Gene therapy using a virus.
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Genomics
Genome
Entire set of genes carried by one member of a
species
Genomics
Study of entire genomes
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Genomics
Human Genome Project
Sequenced all of the DNA in a representative human
cell
Human genome consists of 20,000 to 25,000 genes
Humans are identical in 99.9% of the sequences of
their genes
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Genomics
Microarray
Tool used to analyze gene activity under different
conditions
Thousands of DNA sequences stamped onto a single
glass slide (DNA chip)
Can be used to identify genes that are active in
cancerous cells, but not in healthy cells
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Genomics
Comparisons of genomes of different species
provide evidence of common evolutionary past
We share 50% of our genes with fruit flies
We share 90% of our genes with mice
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Figure 21.19 A comparison of microarrays.
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You Should Now Be Able To:
Know the form of DNA
Know the process of DNA replication
Know all the steps of gene expression
Understand what are mutations
Understand the regulation of gene activity
Understand genetic engineering and genomics