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Operation ManualH:\Shared Data\Heidi\Manuals for Holly\Biohazard Manual\Submitted 10451 Biohazard Cabinet Operation Manual 031120HWJ.doc

BIOLOGICAL SAFETYCABINETMODEL 10451 - EBC 70, 6-Foot Type A2

ENVIRCO™ BIOLOGICAL SAFETY CABINETService and Instruction Manual

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TABLE OF CONTENTS

1.0 Introduction................................................................................................................................................... 11.1. Caution and Warnings ......................................................................................................................... 11.2. Definitions ............................................................................................................................................ 31.3. Description........................................................................................................................................... 31.4. Features............................................................................................................................................... 41.5. Environmental Operating Conditions ................................................................................................... 4

2.0 Installation..................................................................................................................................................... 52.1. Uncrating the Biological Safety Cabinet............................................................................................... 52.2. Cabinet Location.................................................................................................................................. 52.3. Optional Base Stand Installation.......................................................................................................... 62.4. Bench Top Installation ......................................................................................................................... 72.5. Electrical Requirements ....................................................................................................................... 72.6. Warning and Other Identification Label Locations ............................................................................... 82.7. External Exhaust Connection (Optional) .............................................................................................. 9

3.0 Operating Techniques................................................................................................................................ 113.1. Cabinet Operation.............................................................................................................................. 113.2. Surface Decontamination................................................................................................................... 113.3. Surface Maintenance......................................................................................................................... 113.4. Use of Ancillary Equipment................................................................................................................ 113.5. Working in the Biological Safety Cabinet ........................................................................................... 12

4.0 Maintenance and Service........................................................................................................................... 144.1. Filter Repairs ..................................................................................................................................... 144.2. Airflow Adjustment ............................................................................................................................. 15

5.0 Wiring Diagrams ......................................................................................................................................... 17

6.0 Specifications ............................................................................................................................................. 186.1. Cabinet Dimensions ....................................................................................................................... 186.2. Cabinet Drawings ........................................................................................................................... 196.3. Electrical......................................................................................................................................... 206.4. Physical .......................................................................................................................................... 206.5. Lighting........................................................................................................................................... 206.6. High Efficiency Particulate Air (HEPA) Filters:................................................................................ 206.7. Air Flow* ......................................................................................................................................... 21


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7.0 Replacement Parts ..................................................................................................................................... 227.1. HEPA Filter Replacement............................................................................................................... 227.2. Replacement and Optional Parts, Part No. 10451.......................................................................... 23

8.0 Testing and Certification............................................................................................................................ 248.1. Initial On-site Testing and Certification ........................................................................................... 248.2. Recommended Tests at Initial Installation ...................................................................................... 248.3. Cabinet Pressure Testing ............................................................................................................... 248.4. Authorized Service Contractor........................................................................................................ 258.5. Re-certification................................................................................................................................ 258.6. On-Site Testing Procedures ........................................................................................................... 268.7. Post-Installation Testing ................................................................................................................. 27

9.0 Appendix ..................................................................................................................................................... 289.1. Labeling Used on the Cabinet ........................................................................................................ 289.2. Glossary ......................................................................................................................................... 299.3. Risk Levels, Classification .............................................................................................................. 30

10.0 Bibliography................................................................................................................................................ 31

11.0 Manufacturer's Limited Warranty.............................................................................................................. 32


Page 1

INTRODUCTION

1.0 IntroductionInvestigators and technicians concerned with microbiological safety have for many years utilizedspecialized containment enclosures to limit their exposure to harmful pathogens. In addition, itis recognized that exacting research, as well as routine pathology, is often compromised byenvironmental contamination. The ENVIRCO Class II Biological Safety Cabinet is designed tolimit the contamination exposure, when handling biological Safety material, of both the workerand the work.

The Class II enclosure is an open face cabinet that recirculates a high volume of air throughinternal HEPA filters. Air is drawn into the open front of the cabinet and exhausted through aHEPA filter. The controlled airflow direction and volume provides protection for the worker, thework (sample) and from cross contamination within the cabinet

1.1. Caution and Warnings

READ AND SAVE THESE INSTRUCTIONS

CAUTION: To reduce the risk of fire, electrical shock or injury to persons, observethe following:

1.1.1. Read this manual completely before installing or operating the ENVIRCO BiologicalSafety Cabinet.

1.1.2. Use this unit only in the manner intended by the manufacturer. If you have anyquestions, contact the manufacturer:

ENVIRCO CORPORATION FEDDERS INTERNATIONALEnvirco Office China Office5601 Balloon Fiesta Parkway N.E. No. 268 Xi Zang Road (M)Albuquerque, New Mexico 87113, U.S.A. Haungpu DistrictTel : (505) 345-3561 Shanghai 200001Fax : (505) 345-8875 PRCEmail: [email protected] Tel : (86) 512 6258 7180

Fax : (86) 512 6258 0035ENVIRCO CORPORATIONEastern Regional Office FEDDERS INDOOR AIR QUALITY18238 Showalter Road China OfficeHagerstown, Maryland 21742, U.S.A. Factory of SucaiTel : (301) 714-4780 Exp. Process, Zone SuzhouFax : (301) 714-4784 Industrial Park SuzhouEmail: [email protected] Jiangsu, Province

P.R. China 215021TRION INC Tel : (86) 512 625 80030European Operations Fax : (86) 512 625 80031Reith Way, West Portway Industrial EstateAndover, Hampshire, SP10 3TY, EnglandTel : +44 (0) 1264 364622Fax : +44 (0) 1264 350983Email: [email protected]


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1.1.3. Before servicing or cleaning the unit, switch the power off at the facility servicepanel and lock the service panel to prevent power from being switched onaccidentally. Follow any other necessary safety procedures.

1.1.4. The ENVIRCO Biological Safety Cabinet is equipped with a three-wire groundingplug for your protection against shock. Insert the plug into a properly groundedthree-wire outlet. If only a two-wire outlet is available, it must be replaced with aproperly grounded three-wire outlet in accordance with the National Electric Codeand local codes and ordinances. Installation work and electrical wiring must bedone by qualified person(s) in accordance with all applicable codes and standards,including fire-rated construction. Only a qualified electrician should do this work.

5-30 PPlug

5-30 RReceptacle

10451 - EBC 70, 6-Ft Type A2

Figure 1: Plug and Receptacle Requirements

Caution: Do not, under any circumstances, cut or remove the grounding prongfrom the plug.

1.1.5. Unplug the power cord and decontaminate the cabinet before servicing the cabinetor changing the filters. Do not use extension cords. If the power cord or plugbecomes frayed, cracked, broken or otherwise damaged, replace it immediately.

1.1.6. Only an Authorized ENVIRCO Service Contractor should service the cabinet;however, the owner can perform certain operations outlined in the manual. Do notattempt to perform any procedures that are not specifically outlined in this manual.

1.1.7. Do not remove the caution labels from the equipment or the associated work area,except for the instruction label on the view-screen.

1.1.8. Do not remove the front panel or HEPA filters from the ENVIRCO Biological SafetyCabinet until the cabinet has been decontaminated.

1.1.9. Keep this manual for future reference and for training new operators


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1.2. Definitions

1.2.1. Class II, Type A1 Cabinets (formerly designated Type A):

1.2.1.1. Maintain a minimum calculated average inflow velocity of 75 fpm throughthe work area access opening.

1.2.1.2. Have HEPA filtered downflow air that is a portion of the mixed downflowand inflow air from a common exhaust plenum (i.e., a plenum from whicha portion of the air is exhausted from the cabinet and the remainder issupplied to the work area).

1.2.1.3. May exhaust HEPA filtered air back into the laboratory or to theenvironment through an exhaust canopy.

1.2.1.4. May have positive pressure contaminated ducts and plenums that are notsurrounded by negative pressure plenums.

Note: Type A1 cabinets are not suitable for work with volatile toxic chemicalsand volatile radionuclides.

1.2.2. Class II, Type A2 Cabinets (formally designated Type B3):

1.2.2.1. Maintain a minimum average inflow velocity of 100 ft/min (0.5 m/s)through the work access opening.

1.2.2.2. Have HEPA filtered downflow air that is a portion of the mixed downflowand inflow air from a common exhaust plenum.

1.2.2.3. May exhaust HEPA filtered air back into the laboratory or to theenvironment through an exhaust canopy.

1.2.2.4. Have all biologically contaminated ducts and plenums under negativepressure, or surrounded by negative pressure ducts or plenums.

Note: Type A2 cabinets must be exhausted through properly functioningexhaust canopies if they are used for work with minute quantities ofvolatile toxic chemicals and tracer amounts of radionuclides required asan adjunct to microbiological studies.

1.3. Description

1.3.1. The ENVIRCO Biological Safety Cabinet is a Class II, Type A2 unit with HEPAfiltered air recirculation, front air barrier and self-contained exhaust. It provides awork area for safe handling of low to moderate risk biohazard materials. It isdesigned primarily for work in microbiology, virology, tissue culture, and sterilitytesting.

1.3.2. The Biological Safety Cabinet protects the worker from airborne contaminantsgenerated within the work area. It protects the work from airborne contaminantsgenerated in the ambient air and protects against cross-contamination within thework area. The cabinet is designed to comply with NSF1/ANSI Standard 49, ClassII, Biological Safety Cabinetry and bears the NSF seal of compliance.

1 NSF International, Ann Arbor, Michigan 48105


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1.4. Features

1.4.1. The hinged viewscreen is constructed with safety glass and has a seven-degreeangle for comfortable viewing. The hinge enables easy loading and unloading ofwork and allows maximum accessibility when cleaning the interior work area.

1.4.2. The all stainless steel work area has a lift-out work tray and support for easycleaning.

1.4.3. The self-contained drain pan with external drain valve simplifies flushing andcleanup of major spills.

1.4.4. The electrical and air, gas or vacuum outlets within the work area include allinternal plumbing.

1.4.5. The exhaust flow buzzer warns the operator in the event of loss of containmentfunction.

1.4.6. All of the controls, light tubes, and serviceable components are located outside thecontaminated area within a convenient, hinged front panel.

1.4.7. The heavy duty and stable work deck support provides extremely low vibrationtransmission.

1.4.8. The leak-tight cabinet is designed to provide negative pressure. None of thecontaminated air plenums are at positive pressure with respect to the laboratory.

1.4.9. The price of the cabinet includes initial on-site testing and certification of properperformance by an Authorized Service Contractor.

1.5. Environmental Operating Conditions

1.5.1. Indoor Use Only.

1.5.2. Altitude up to 2000 meters (6,562 ft)

1.5.3. Room temperatures must be maintained between 41-104°F (5-40 °C)

1.5.4. Maximum relative humidity of 80% for temperatures up to 88°F (31°C), thendecreasing linearly to 50% at 104°F (40°C).

1.5.5. Incoming power must be maintained within 120 ±10 Volts.


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INSTALLATION

2.0 Installation

2.1. Uncrating the Biological Safety Cabinet.

Note: To prevent accidental damage, do not remove the Biological Safety Cabinet fromits shipping skid until the unit is ready to be iinstalled at its final location.

2.1.1. Carefully unpack the Biological Safety Cabinet and inspect for damage that myhave occurred in transit. If the unit is damaged, notify the carrier immediately andretain the entire shipment intact for inspection by the carrier.

2.1.2. Move the unit by placing a flat, low dolly or pallet jack under the shipping skid.

2.1.3. Removing the shipping skid

2.1.3.1. First remove the plastic wrapping and then the corner posts.

2.1.3.2. Verify that an appropriately sized bench top is available or the optionalBase Stand is positioned in the final location.

CAUTION: The Biological Safety Cabinet is very top heavy so extremecaution should be used when lifting the cabinet onto the stand orthe bench top.

2.2. Cabinet Location

2.2.1. The cabinet should be placed in an area of the laboratory free from strong aircurrents, heavy traffic and particulate microbial or chemical contamination. This willreduce any disruption of the airflow patterns at the face opening and will prolongthe life of the HEPA filters.

2.2.2. The ceiling height above the cabinet should allow four (4) inches of clearanceabove the exhaust area to allow free air passage.


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2.3. Optional Base Stand (#10277) Installation

2.3.1. Place the base stand in the desired location (Figure 2).

2.3.2. Carefully lift the cabinet onto the top rails of the Base Stand and slide into place.

2.3.3. Insert the bolts to secure the Biological Safety Cabinet to the Base Stand.

2.3.4. Level the Base Stand as necessary using metal shims at the bottom of the stand.

2.3.5. Silicone caulking must be applied along the bottom of the stand sides to seal thearea between the floor and the stand.

2.3.6. Thread the drain valve in place and tighten.

2.3.7. Put the work deck support and pan in place.

Figure 2: Base Stand Installation


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2.4. Bench Top Installation

2.4.1. Set the cabinet on the bench top with five (5) inches overhanging in the front(Figure 3.)

2.4.2. Thread the drain valve in place and tighten.

2.4.3. Put the work deck support and pan in place.

2.4.4. Level the work deck by placing metal shims under the bottom of the cabinet.

2.4.5. Silicone caulking must also be applied to seal the area between the bench top andthe cabinet.

Figure 3: Bench Top Installation

2.5. Electrical Requirements

2.5.1. Ensure that a 30 amp, 115V electrical outlet with the proper receptacle is availablefor the EBC 70, 6-Ft Type A2 cabinet.

2.5.2. Connect the air, gas or vacuum service line to the external connection pointlocated on the top of the cabinet.

Note: The connections should be field tested for leak tightness.


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2.6. Warning and Other Identification Label Locations

See Page 29 for descriptionof all labeling used on theCabinet.

Service ConnectionWarning

Biohazard Warning

Service Card and Holder

Switch and Data Plate

Circuit Diagram (inside)

NSF Seal

Certification Warning

Flammable Materials Warning

FRONT VIEW

Figure 4: Top and Front Cabinet Views with Label Locations


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2.7. External Exhaust Connection (Optional)

Under normal conditions, the Class II Type A2 cabinets may exhaust HEPA filtered airback into the laboratory and the exhaust thimble provided with the unit does not have to beinstalled. However, when the Class II Type A2 cabinet is used for work with minutequantities of volatile, toxic chemicals or trace amounts of radionuclides, the unit must beconnected to an external laboratory exhaust system with the use of the exhaust thimbleconnection. This ensures that the exhaust air balance of the Biological Safety Cabinet isnot disturbed by fluctuation in the external exhaust system.

In order to assure a negative pressure exhaust system, the external exhaust capacityshould be sized as follows:

10451 - EBC 70, 6-Ft Type A2: 460-cfm minimum

Since the exhaust transition is a thimble connection, the exhaust motor/blower need onlybe sized to overcome the resistance of the facility exhaust system.

Note: The customer must install the thimble connector suspension and provide theexternal exhaust system fan and damper

Figure 5: External Exhaust Through Ceiling


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Top View

10451 - EBC 70, 6-Ft Class II, Type A2

Figure 6: Thimble Exhaust as Viewed from Front of Unit


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OPERATING TECHNIQUES

3.0 Operating Techniques

3.1. Cabinet Operation

After the cabinet has been properly installed and certified, it is ready for use. The threeswitches on the control panel operate the cabinet blower system, fluorescent lights, andconvenience outlet.

3.2. Surface Decontamination

To disinfect the work area before use, turn the blower system "ON," raise the viewscreenand secure it with the bead chain holder. Wipe the stainless steel and all work areacomponents, including the inside of the viewscreen, with a fast-drying, non-corrosivedisinfectant. Remove the two screws located at the two front corners of the perforateddiffuser grille above the work area. Carefully remove the grille and clean it on both sides.Do not attempt to clean the bottom of the HEPA filter since any contact with the filtersurface may cause damage and contamination leakage.

After the cabinet is turned “OFF,” the work surfaces should be cleaned with a disinfectant.The work tray, front air grille, and work tray support/rear air grille can be removed tofacilitate the clean up of any spillage.

An optional UV light accessory (#S22557) can be used for interior work surfacedecontamination. The UV light accessory fits into the face opening and the power andswitching function is supplied from the convenience outlet within the cabinet.

3.3. Surface Maintenance

Painted surfaces and the outside of the safety viewscreen may be cleaned with soap andwater. A light abrasive soap can be used on the stainless steel areas to help removescratches and stains. Rub in the direction of the grain only.

3.4. Use of Ancillary Equipment

Note: Do not use equipment requiring more than three (3) Amps.

3.4.1. Bunsen Burner

Caution: Bunsen Burners should never be operated unless the cabinet blowers areturned “ON.” The use of flammable materials within the cabinet is notrecommended. Bunsen Burners must be operated so as to preventunburned gas mixture collection in the cabinet.

If a flame is required within the work area, a small "touch-light" type should be usedto minimize the heat build-up and subsequent operator discomfort that acontinually burning flame may create. To avoid exposing an open flame within thework area, an incinerator-type sterilizer is recommended for sterilizing inoculatingloops.


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3.4.2. Blender

Caution: Be sure that the unit is operating before starting either a centrifugeor blender. The cabinet must continue to operate for a minimumof five (5) minutes after the appliance is turned “OFF.”

A blender may be operated in the unit. Because of the severity of the aerosolgenerated, however, it is suggested that the operator keep his or her arms, otherequipment and containers out of the work area during this procedure.

3.5. Working in the Biological Safety Cabinet

Caution: Because of the highly specialized nature of the ENVIRCO BiologicalSafety Cabinet, it is recommended that an Authorized Service Contractorperform all service and maintenance. A maintenance and servicedecontamination procedure is available upon request.

3.5.1. The cabinet is ready to use only after an Authorized ENVIRCO Service Contractorhas completed on-site testing and certification procedures.

3.5.2. All equipment should be clean before being placed inside the cabinet work area.

3.5.3. Close the viewscreen and secure it in the working position.

3.5.4. Turn on the lights and blowers with the switches located on the control panel andwait until the airflow reaches an acceptable speed.

3.5.5. Test for airflow containment by holding a smoke source in front of the cabinetopening. This simple visual smoke test is described in section 8.7.1. Thisprocedure may be repeated daily, however, routine re-certification of the cabinetis always recommended.

3.5.6. Allow the cabinet to run for five (5) minutes in order to remove any airborneparticulate material from the system before beginning work.

3.5.7. Work procedures should be conducted only on the solid, non-perforated worksurface.

Caution: To maintain proper airflow and reduce air turbulence, do notobstruct the front and rear return air grilles.

3.5.8. All equipment and containers should be placed in the cabinet before work isbegun.

Caution: The front viewscreen must never be lifted during a procedure.This may disrupt the airflow characteristics of the cabinet, mayallow contaminated air to leave the cabinet or may allow room airto enter the cabinet.

3.5.9. Unnecessary items should not be stored in the work area.

3.5.10. The cabinet does not have the ability to remove contaminants from its surfaces.To prevent surface-to-surface contamination, clean all equipment using propersafety and clean handling techniques.


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3.5.11. The operator's hands and arms should be placed into and withdrawn from thework area slowly, thus preventing "dragging" of air from one environment to theother. Laboratory coat sleeves and cuffs should fit snugly on the operator's armsto prevent trapping contaminated air entering the front return grille. Surgicalgloves are recommended while working in the cabinet.

3.5.12. After the work procedure is completed and the containers of biohazardousmaterial are closed and sealed, the cabinet should be allowed to run for five (5)minutes to ensure removal of airborne contamination within the cabinet before theequipment and containers are removed.

Note: If the normal daily working routine in the cabinet includes constantexposure of low to moderate risk level agents, the cabinet should beoperated continuously, 24-hours a day, seven (7) days a week.


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MAINTENANCE AND SERVICE

4.0 Maintenance and Service

Caution: If the cabinet has been used with infectious agents, decontamination isnecessary before removing access panels.

4.1. Filter Repairs

The shipping and handling process may cause filter leaks. These leaks can normally berepaired as follows:

4.1.1. Caulking with silicone RTV can usually repair small leaks in the filter media or at anadhesive joint from the downstream side of the HEPA filter.

4.1.2. RTV sealant may be applied on the downstream side of the HEPA filter for leaks atthe gasket seal. If these leaks cannot be stopped from the downstream side, thecabinet access panels must be removed and the leaks repaired from the upstreamside.

4.1.3. To seal major leaks in the gasket or filter media, first remove the exterior accesspanel and then the inner filter access panel to expose the upstream side of thefilter. Remove the HEPA filter and seal the leaks by pouring rubber base cementdown into the leaking area. Check for separation at the gasket corners and repairwith silicone RTV. Apply silicone grease to the gasket surface before re-installingthe filter to improve the seal.

4.1.4. Re-install the filter access panels, and retest. Put silicone grease on the exteriordoor gasket before re-installing on the cabinet. Do not over-tighten the accesspanel screws. Replace the tubing on the magnehelic gauge and reconnect thewarning buzzer. Replace the filter screen and exhaust damper housing. Set thedamper with blades about 3/8 inch open to begin airflow adjustment.

Figure 7: Supply HEPA Filter


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4.2. Airflow Adjustment

Airflow adjustments can be made with either the solid state speed control inside theelectrical control panel and/or with the exhaust damper. Access to the damper adjustingrod is gained through the plugged access hole located in the exterior access panel of thecabinet. The recirculating supply airflow velocity and the exhaust airflow velocity must beset to the values in Section 6.7 of this manual. The recirculating supply and exhaustairflows are interactive; therefore some re-adjustments may be required if either ischanged significantly.

4.2.1. Equipment

4.2.1.1. An airflow thermal anemometer with sensing probe eight inches or longerand less than ½-inch in diameter.

4.2.1.2. A Shortridge Airdata Multimeter ADM-870 with 8” x 24” hood isrecommended, but not required.

4.2.2. Downflow - Recirculating Supply Airflow Measurement

The recirculation supply air velocity is measured within the work area enclosed bythe perimeter walls and viewscreen in the horizontal plane four inches above thebottom edge of the viewscreen. Measurement points are shown in Figures 8-9.Measure and record all points and calculate the overall average zone or averagevelocities. Adjust the solid state speed control and/or exhaust damper as requiredto achieve averages within the above stated limits.

Rear of work deckProbe set at four inches above the bottom edge of the window

Figure 8: Downflow Velocity Grid, Models 10451 – EBC 70, 6-Ft Type A2

Service and Instruction Manual

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4.2.3. Inflow Airflow Measurement

4.2.3.1. Direct Inflow Method (Recommended)

The inflow airflow is measured by sealing the 8” x 24” hood directly intothe window and blocking off the entire remaining opening. Takemeasurements with the Shortridge device and adjust the exhaust airflowwith the interior damper and the solid state speed control as required.

4.2.3.2. Calculated Method

The exhaust airflow is measured four inches above the face of theexhaust filter, level with the top plane of the exhaust housing. Takemeasurements at approximate locations as depicted by the exhaustvelocity grids shown below. Adjust the exhaust airflow with the interiordamper and/or the solid state speed control.

Exhaust Velocity Grid10451-EBC 70, 6-Ft Type A2

Figure 9: Exhaust Velocity Grid


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WIRING DIAGRAMS

5.0 Wiring Diagrams

Figure 10: 10451 - EBC 47, 6-Ft Type A2 Wiring Diagram


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SPECIFICATIONS

6.0 Specifications

6.1. Cabinet Dimensions

Model A B C D E F G H

10451 – EBC 70, 6-Ft Type A2 70.0” 32.5” 62.0” 58.0” 4.0” 28.5” 32.0” 28.5”

Figure 11: Biological Safety Cabinet Dimensions


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6.2. Cabinet Drawings

Figure 12: 10451 – EBC 47, 6-Ft Type A2 Cabinet Drawing


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6.3. Electrical

10451 – EBC 70, 6-Ft Type A2: 115v, 60 Hz, 20 amp

Single power cord with a NEC 5-30p cap plug

Two (2) 1/3 h.p. motors, electrically commutated,thermally protected, digital speed control

Note: All models have separate combination circuit breaker/switches for the motor(s),lights and duplex electrical outlet.

6.4. Physical

Dimension 10451 – EBC 70, 6-Ft Type A2

Overall Height 62.0 in. (157.5 cm.)

Overall Depth 32.5 in. (82.6 cm.)

Overall Width 70.0 in. (177.8 cm.)

Work Area Height 26.0 in. (66.0 cm.)

Work Area Depth 21.0 in. (53.3 cm.)

Work Area Width 68.5 in. (174.0 cm.)

Face Opening Height 8.0 in. (20.3 cm.)

Shipping Weight – Cabinet 750 lbs. (340.2 kg.)

6.5. Lighting

The light intensity at the work surface is between 80-150 foot-candles provided bytwo (2) fluorescent light tubes controlled by a separate breaker switch.

6.6. High Efficiency Particulate Air (HEPA) Filters:

Both the recirculating supply air and exhaust air filters remove all particles 0.3 µm andlarger in size with an efficiency of 99.99%. The HEPA filters are mounted with acompression spring clamping system.


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6.7. Air Flow

6.7.1. 10451 - EBC 70, 6-Ft Type A2

Direct Inflow MethodVelocity 100 – 110 fpmAirflow 381 – 419 cfm

Calculated Face Velocity 115 – 125 fpmExhaust Volume 438 – 476 cfmExhaust Filter Area 2.33 sq. ft.Front Access Area 3.81 sq. ft.

Downflow VelocityUniform 65-75fpm

ZonedZone 1 (Back 3 Rows) 68 - 78 fpmZone 2 (Front) 56 - 66 fpm

Notes:

1. In performing the velocity profile test, the front row of velocity readings (closest to theviewscreen) must be taken with the thermoanemometer probe positionedperpendicular to the viewscreen with the probe head facing the viewscreen.

2. Velocity readings for the two middle rows and rear row must be taken with the probepositioned parallel to the viewscreen.

3. In performing the calculated face velocity test, the exhaust transition collar must beremoved when taking air velocity readings using a thermal anemometer probe.

4. The Direct Inflow Method is the manufacturer’s recommended method for balancingthe 10451-EBC 70 Biological Safety Cabinet.

5. Adjustments to the airflow or air velocity are made by rotating either the CourseAdjustment Control or the Fine Tuning Control. The Course Adjustment Controlchanges the airflow by approximately 10% or roughly 10 fpm per increment while theFine Tuning Control adjusts the airflow by approximately 1% percent per increment.Figure 13 below shows the adjustment face of the Digital Speed Controls.

Figure 13: Digital Speed Controls


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REPLACEMENT PARTS

7.0 Replacement Parts

Caution: Do not remove the front panel until the cabinet has beendecontaminated.

7.1. HEPA Filter Replacement

The HEPA filters require replacement when the air resistance is such that the specified airvelocities cannot be achieved. Replacement should also be made when the HEPA filter isdamaged beyond repair or if three percent or more of the filter surface has been patched.Only an Authorized Service Contractor should replace the HEPA filters.

7.1.1. Filter Removal

7.1.1.1. Remove the exterior access panel by removing the holding screws. Next,remove the lower, inner panel(s) to gain access to the supply air HEPAfilter. Finally, remove the upper, inner panel to gain access to theexhaust HEPA filter.

7.1.1.2. Loosen the four (4) bolts holding the spring clamp on each filter. Removethe bolts, springs and bars. The exhaust filter will drop down for easyremoval. The supply air filter may be lifted out of the cabinet. All usedfilters should be disposed of by incineration.

7.1.2. Filter Installation

7.1.2.1. Install the new filters in the same way as the old filters were removed.The filter media is very fragile and even slight finger pressure on the filterpack itself may cause damage. Handle the filters by the aluminum frameedges only. Tighten the spring clamps until the spring is not quite fullycompressed. Do not over-tighten.

7.1.2.2. Replace all access panels. A light coat of silicone grease should beplaced on the front access panel gasket before replacing. If the gasketwas damaged upon removal, install a new gasket before reinstalling thepanel.

7.1.2.3. After the HEPA filters have been replaced, a filter leak test procedure(Section 8.6) should be performed to verify the filter seals, and a cabinetpressure leak test (Section 8.3) should be performed to verify the accesspanel seal. The airflow velocity should also be checked and adjusted asnecessary.


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7.2. Replacement and Optional Parts, Part No. 10451

7.2.1. 10451-EBC 70, 6-Ft Type A2

Description Part Number

Motor/Blower Assembly 22425-002 (2 ea.)Motor, 1/3 H.P. O.D.P., 1400 rpm 63589 (2 ea.)Digital Speed Control 63672Transformer- Class 2 63667Buzzer 90902Breaker Switch, 3 amp 61865 (2 ea.)Breaker Switch, 10 amp 62372Lamp, F65T12CW 61660Viewscreen 22880Filter, 18" x 24" x 12" 61702Filter, 18" x 34" x 6" 61701 (2 ea.)Magnehelic Gauge, 0-2" 60899Diffusion Screen 225463/8" Ball Valve Drain 62560Service Record Card 61246Gasket, Closed, 1/4" x 3/4" 60105Assembly, Ballast 32151Bead Chain with hook 22471Exhaust Thimble 10667UV Light Accessory 22557

Parts can be ordered from:ENVIRCO CORPORATION5601 Balloon Fiesta Parkway N.E.Albuquerque, New Mexico 87113, U.S.A.Tel : (800) 545-6598Fax : (505) 345-8875


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TESTING AND CERTIFICATION

8.0 Testing and CertificationThe ENVIRCO Biological Safety Cabinet has been fully tested at the Factory prior to shipment,however, shipping and handling during installation may result in HEPA filter leaks or othercabinet seal leaks. Each cabinet must be adjusted for proper airflow balance after it has beeninstalled in its final location or if whenever the cabinet is moved to another location.

8.1. Initial On-site Testing and Certification

Note: Operation of the cabinet prior to authorized certification may void the warranty andservice contract or result in additional service contractor charges.

Initial on-site testing and certification of the ENVIRCO Bio Cabinet is included with unitspurchased within the Continental United States. Contact an Authorized Service Contractorto arrange for certification. Initial on-site testing and certification services includes basicoperation and maintenance training for the unit and validation of the manufacturer'swarranty.

8.2. Recommended Tests at Initial Installation

Note: The following tests are recommended at initial installation

8.2.1. Aerosol Leak Test of the recirculating air HEPA filters.

8.2.2. Aerosol Leak Test of the exhaust air HEPA filter.

8.2.3. Adjustment of the airflow within the cabinet to an average velocity per values inSection 6.7 of this manual.

8.2.4. Adjustment of the exhaust air volume to provide the required inward airflow (facevelocity) values listed in Section 6.7.

8.2.5. Visually verify that an inward airflow exists around the entire periphery of the faceopening using an acceptable smoke challenge (see section 8.7.1).

8.2.6. Check electrical continuity between cap plug ground pin and exposed metalcabinet surfaces to verify that electrical resistance does not exceed 0.15 ohms.

8.3. Cabinet Pressure Testing

The ENVIRCO Biological Safety Cabinet is designed so that there are no contaminatedplenums under positive pressure when the cabinet is operating. The entire outer shell ofthe cabinet and the work area are under negative pressure with respect to the laboratory.Therefore, any cabinet penetration leaks or access panel leaks are "inward" and thus not acontamination hazard.

The exterior access panel is sealed and the cabinet designed to be airtight at two (2)inches water gauge over-pressure when the open face and the exhaust openings aresealed off. A test of the cabinet seals after removal of the access panel can beaccomplished with the following procedure:


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8.3.1. Suggested Equipment

8.3.1.1. Heavy vinyl sheeting and two-inch plastic or rubberized tape to seal off allcabinet openings.

8.3.1.2. A small compressor or the building’s compressed air source.

8.3.1.3. A detergent solution or other liquid leak detector solution in a hand-heldspray bottle.

8.3.2. Test Procedure

Note: The Electrical Control Compartment may be completely removed byunplugging the wiring spade connectors from the terminal block on the rightside and removing the hinge screws. Unplug the power cord beforeattempting to remove the compartment.

8.3.2.1. Seal off the front and exhaust openings to the cabinet using tape and thevinyl sheeting.

Note: A complete, pressure-tight seal must be formed before proceedingto the next step.

8.3.2.2. Connect the pressure source to the drain valve fitting located at thebottom, left side of the cabinet. The valve can be used to control the airpressure feed into the cabinet.

8.3.2.3. Pressurize the cabinet to two-inch water gauge, using the magnehelicgauge mounted on the front of the unit to monitor the pressure. Spray asoap solution around the access panel opening and at all other cabinetpenetrations. The formation of soap bubbles indicates an air leak thatmust be sealed off by tightening the fasteners or by using a siliconerubber, construction joint adhesive.

8.4. Authorized Service Contractor

ENV Services, Inc.(800) 345-6094

8.5. Re-certification

It is recommended that the ENVIRCO Biological Safety Cabinet be re-certified every six(6) months and the HEPA filters replaced if necessary. An Authorized Service Contractorcan provide this service upon request and should be contacted directly.


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8.6. On-Site Testing Procedures

8.6.1. HEPA Filter Leak Testing

The approved testing method requires challenging the integrity of the HEPA filterswith a cold polydispersed aerosol2 introduced upstream from the HEPA filters. Alight scattering photometer detects any aerosol penetration at the downstreamsurface of the filter.

8.6.2. Test Equipment Required

8.6.2.1. Air operated “Laskin Nozzel” type aerosol generator to produce anaerosol with a mean particle size of 0.6 micron

8.6.2.2. Light Scattering Aerosol Photometer

8.6.2.3. Silicone RTV

8.6.2.4. Rubber-base solvent cement

8.6.2.5. Silicone stopcock grease

8.6.3. Preparation

3.6.3.1. Expose the Supply HEPA Filter by removing the perforated aluminumdiffuser screen located directly above the work area.

3.6.3.2. Open the electrical control panel by removing two bolts at each end. Pullthe rubber tubing off the magnehelic gauge.

3.6.3.3. Disconnect warning buzzer at left-hand side of harness.

3.6.3.4. Remove the work tray.

3.6.3.5. Turn cabinet blower "ON."

3.6.3.6. For optimal mixing, attach the aerosol generator output hose so that theaerosol is introduced into the return air plenum at the back, center of thework area.

8.6.4. Testing

8.6.4.1. Calibrate the photometer with the aerosol generator so that the upstreamconcentration is 100 percent. The upstream concentration for all HEPAfilters may be sampled by removing the pipe plug from the penetrationprovided on the top front of the cabinet and inserting the photometerprobe in the penetration fitting. Set the photometer to ZERO and verifythe 100 percent upstream and zero downstream readings are achieved.Set the range switch to read a minimum of 0.01 percent.

8.6.4.2. Carefully scan the faces of the recirculating supply and exhaust filters,particularly around the edges of the sealing gaskets and adhesive seal.Hold the photometer probe about one inch from the filter surface andscan at about two inches per second.

8.6.4.3. Identify and repair any leaks greater than 0.01%.

2 NSF/ANSI 49-2002


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8.7. Post-Installation Testing

8.7.1. Visual Containment

After the cabinet has been balanced for proper air velocity readings, it should bechecked with a simple visual smoke test.

8.7.1.1. Hold the smoke source outside the cabinet face opening to ensure thesmoke is pulled into the opening around all four (4) sides. Hold thesmoke source three (3) to four (4) inches away from cabinet and scanaround the entire opening.

8.7.1.2. Hold the smoke source inside the work area behind the glass viewscreenand confirm that no smoke escapes the front opening. Hold source four(4) inches behind the viewscreen and about 12 inches above the workdeck. Scan the cabinet from side-to-side.

8.7.1.3. Hold the smoke source inside the work area along the front to backcenterline of the work deck and about 12 inches above the work deck.Check the front to back split of the airflow across the work deck near thecenter of the cabinet.

8.7.2. Electrical Tests

Electrical grounding tests should be made to assure that all exposed metalsurfaces of the cabinet are grounded.


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APPENDIX

9.0 Appendix

9.1. Labeling Used on the Cabinet

Review all labeling on the ENVIRCO Biological Safety Cabinet. It provides both theinstaller and the worker with important information regarding cabinet function and safety.

WARNINGService Connection(s)

If not used, leave cap plugsin place to maintain sealedcabinet integrity

IMPORTANTDo Not Plug in Cabinet.

Do Not Use Cabinet Until Certification Has BeenCompleted.

Verify That 115V Electrical Receptacle, NEMA Type 5-30R is Available and Located Within Reach of CabinetPower Cord.

Service ConnectionWarning

NSF SealCALL FOR CERTIFICATIONContact: ENV Services, Inc.

1016 W. Eighth AvenueKing of Prussia, PA 19406(800) 345-6094

Certification Warning

Service and Instruction Manual

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9.2. Glossary

Aerosol: A colloid of liquid or solid particles suspended in a gas, usually air.

Ambient: Surrounding on all sides.

Biohazard: A contraction of the words biological and hazard. Infectious agent(s), or partthereof, presenting a risk or potential risk to the well being of man, or other animals, eitherdirectly through infection of indirectly through disruption of the environment.

Biosafety Level: The combination of laboratory practices and techniques, safetyequipment, and laboratory facilities appropriate for the operations performed and thehasard posed by the infectious agents and the laboratory function or activity. Thesebiosafety levels are described in Biosafety in Microbiological and BiomedicalLaboratories3.

Carcinogen: A substance or agent producing or inciting cancer.

Etiologic: Relating to all of the causes of a disease of abnormal condition.

HEPA Filters: High efficiency particulate air filters. These have a nominal efficiency of99.99% removal of 0.3 micron particles from air.

Laminar Air Flow: Air flow in which the entire body of air within a designated spacemoves with uniform velocity in a single direction along parallel flow lines.

Plenum: A chamber for conveying or containing air.

Linear Feet Per Minute (lf/m): A unit of measurement for air velocity.

NSF/ANSI Standard 49: NSF International standard for Class II (Laminar Flow) BiosafetyCabinetry. This standard covers cabinetry design to minimize biohazards inherent in workwith low and moderate risk biological agents. It does not cover equipment included for usewith high risk biohazards. It includes the basic requirements of design, construction, andperformance necessary for reliability of function and safety, and adequate personnel andproduct protection.

NCI: National Cancer Institute.

NIH: National Institutes of Health.

Oncogenic: Tending to cause the formation of tumors.

OSHA: Occupational Safety and Health Administration or Act.

3 U.S. Department of Health and Human Services, DHHS Publication CDC 93-8395, U.S. Government Printing Office, Washington, DC 20402.


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9.3. Risk Levels, Classification

9.3.1. Low Risk

The risk level of the agents and/or the operation has minimal effect on personnel,animals, or plants under ordinary conditions. This classification is restricted to alletiological agents designated Class I by the U.S. Department of HEW, Center forDisease Control.

9.3.2. Moderate Risk

The risk level of the agents and/or the operation requires special conditions forcontrol or containment because of:

9.3.2.1. Known pathogenicity to personnel, animals, or plants.

9.3.2.2. Concentration.

9.3.2.3. Genetic alterations and synergistic effects with other materials.

This classification includes all etiologic agents designated Class II or Class III bythe U.S. Department of HEW, Center for Disease Control and oncogenic virusesspecified as moderate risk by the National Cancer Institute.

9.3.3. High Risk

The risk level of the agents and/or the operations requires additional controlmeasures beyond those for moderate risk. These are agents or operations withany of the following characteristics:

9.3.3.1. Infections can be produced by low doses.

9.3.3.2. Pose a high mortality risk.

9.3.3.3. May potentially spread outside the laboratory.

9.3.3.4. Dangerous concentrations.

9.3.3.5. May release microbial aerosols.

9.3.3.6. May result in genetic alteration or genetic recombination that significantlyincrease the potential for pathogenicity or spread.

This classification includes all etiological agents designated Classes IV and V bythe U.S. Department of HEW, Center for Disease Control, and oncogenic virusesclassified as high risk by the National Cancer Institute.


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BIBLIOGRAPHY

10.0 Bibliography

1. Effective Use of the Laminar Flow Biological Safety Cabinet. U.S. Department of Health,Education and Welfare, Office of Biohazard and Environmental Control, Bethesda, MD.

2. Federal Standard No. 209B, U.S. Government Printing Office, 1973, Superseding FederalStandard No. 209A, 1966.

3. National Cancer Institute Safety Standards for Research Involving Chemical Carcinogens,DHEW Publication No.(NIH) 76-900, 1975.

4. NSF/ANSI 49-2002 for Class II (Laminar Flow) Biosafety Cabinetry, 2002.

5. U.S. Department of Health, Education and Welfare, Public Health Service Center forDisease Control, Classification of Etiologic Agents on the Basis of Hazard, 4th Edition, July1974.

6. U.S. Department of Health, Education and Welfare, Public Health Service, NationalInstitutes of Health, Certification of Class II (Laminar Flow) Biological Safety Cabinets.

7. National Cancer Institute Safety Standards for Research Involving Oncogenic Viruses,DHEW Publication No.(NIH) 75-790.

8. DHEW, National Institutes of Health, Recombinant DNA Research Guidelines, FederalRegister, July 7, 1976.


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LIMITED WARRANTY

11.0 Manufacturer's Limited WarrantyThe Biological Safety Cabinet is manufactured by ENVIRCO, of Albuquerque, New Mexico andis warranted to be free from defects in material and workmanship under normal use. Set forthbelow is information concerning the duration and extent of this warranty, qualifications,conditions and limitations of the warranty, the procedure to be taken to exercise the warranty,and other information concerning the warranty policy.

This warranty is valid only in the 48-contiguous United States covering parts and labor only, andextends for a period of three (3) years from the date of on-site certification by the manufactureror his Authorized Service Contractor. This warranty applies only to the original purchaser of theENVIRCO Biological Safety Cabinet. The fluorescent light tubes (and optional ultraviolet lamp)are not covered by this warranty. Defects, malfunctions, failure or damage of the ENVIRCOBiological Safety Cabinet caused by improper, unreasonable, or negligent use or abuse by theconsumer are excluded from this warranty. This warranty is also void if the serial number hasbeen removed or the cabinet has been damaged.

To secure performance of this warranty and repair of the ENVIRCO Biological Safety Cabinet,the following procedures should be taken:

1. To put this warranty into effect the ENVIRCO Biological Safety Cabinet must be tested andcertified, on-site, by the manufacturer or his Authorized Service Contractor.

2. To make repairs or replacements within the terms of this warranty, contact:

ENV SERVICES, INC.(800) 345-6094

Upon compliance with the above procedure, all warranted defects will be repaired (at noadditional charge to the consumer) as long as the Biological Safety Cabinet has not beendamaged, tampered with or negligently used as described above.

ENVIRCO makes no warranty expressed or implied except as stated above. For ENVIRCOBiological Safety Cabinets sold or located outside the 48-contiguous United States, ENVIRCOassumes liability only for the replacement of defective part(s), excluding labor.

Note: On-Site Testing and Certification of the ENVIRCO Biological Safety Cabinet by themanufacturer or his Authorized Service Contractor is a precondition to warranty serviceor performance. Under no circumstances should the user operate the ENVIRCOBiological Safety Cabinet prior to its on-site certification.

THIS CABINET MAY BE RESTRICTED TO USE INSPECIALLY DESIGNED FACILITIES UNDER

STATE, FEDERAL, OR LOCAL CODES.


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MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. IN NO EVENTSHALL ENVIRCO BE LIABLE FOR PUNITIVE, INCIDENTAL, OR CONSEQUENTIALDAMAGES ARISING OUT OF THIS SALE, INCLUDING, BUT NOT LIMITED TO DAMAGETO A PERSONS OR PROPERTY, LOSS OF USE, LOSS OF TIME, INCONVENIENCE,EQUIPMENT RENTAL, LOSS OF EARNINGS OR PROFIT OR ANY OTHER COMMERCIALLOSS. THIS WARRANTY EXCLUDES CERTAIN EXPENDABLE ITEMS SUCH ASPREFILTERS, ETC. ENVIRCO EXPRESSLY DISCLAIMS AND EXCLUDES FROM THEWARRANTY ANY RESPONSIBILITY FOR EQUIPMENT FAILURES AND/OR DEFECTSATTRIBUTABLE TO IMPROPER MAINTENANCE, ABUSE, ACCIDENT ORMODIFICATION OF EQUIPMENT (SUCH AS APPLICATION OF AN ADJUSTABLEFREQUENCY DRIVE).

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