biological laboratory safety
DESCRIPTION
Biological Laboratory Safety. Additional Information. Where to start. Before looking at this presentation, ensure that you have watched the one on General Laboratory Safety. What are the additional hazards from using Biological Materials?. Infectious agents Toxins Prions Bacteria Viruses. - PowerPoint PPT PresentationTRANSCRIPT
Biological Laboratory SafetyBiological Laboratory Safety
Additional Information
Where to start
Before looking at this presentation, ensure that you have watched the one on General Laboratory Safety
What are the additional hazards from using Biological Materials?
Infectious agents Toxins Prions Bacteria Viruses
What legislation applies?
The main pieces of legislation are: Genetically Modified
Organisms (Contained Use) Regulations 2000
Control of Substances Hazardous to Health Regs 2004
Each will be summarised separately
Control of Substances Hazardous to Health Regs 2004
Four Hazard Groups No hazard group 4 (highest
risk) work in Sheffield Work on hazard group 3
requires special training and Level 3 containment facilities
Risk assessments to be submitted to local LGMSC for scrutiny for all GM work and work with dangerous pathogens
Appropriate waste segregation required
Genetically Modified Organisms (Contained Use) Regulations 2000
All work to be risk assessed using the HSE risk assessment form and be submitted to the Local Genetic Modification Safety Committee before work starts
Approval & notification procedures
Workers to submit health declarations to Occupational Health
Appropriate waste segregation
Protective Clothing
An appropriate laboratory coat must always be worn, fastened up and covering all normal clothing
Lab coats must be decontaminated before laundering
Appropriate eye protection must be worn
Gloves must be worn for work and must be removed when handling taps, note books etc and before leaving the laboratory
Blood & bodily fluids
Use screened animal blood, rather than human blood if possible
Failing this use only screened human blood
All blood and bodily fluids should be considered potentially infectious and treated with due care
Injuries involving body fluids must be reported to your supervisor IMMEDIATELYIMMEDIATELY
Spillages
Report Wear protective clothing Clean up Decontaminate – ensure
that you always have material available to do so
Ensure waste is disposed of properly
You spill = you clean up!You spill = you clean up!
Laminar Flow Cabinets
Laminar flow cabinets are designed to provide a clean-air environment to protect the work
They DO NOT provide They DO NOT provide protection for the protection for the operatoroperator
Biological Safety Cabinets Class 1
Some protection to user & environment
No protection to work Class 2
Good protection to user, the environment and the work
Class 3 Total enclosure
You must choose the correct safety cabinet for the job you are doing. Seek advice from your Biological Safety Officer
Autoclaves
Potentially dangerous equipment
Require regular maintenance and an annual statutory examination
Discard autoclaves must be calibrated every 6 months
You must be trained to use them
Disposal of Waste
Make sure you follow the University policy carefully
Ask your supervisor or a senior member of technical staff if you do not know the correct disposal method for the materials with which you are working
When in doubt – ASK!!!
Do not carry out a new or unfamiliar procedure until you have been fully trained & understand the precautions necessary for safe working
DO NOT GUESS!!!!DO NOT GUESS!!!!