biolamina technology presentation
TRANSCRIPT
Best support for you,and your cells
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Tuesday, August 31, 2010
Current pharmaceutical methods are not impacting major disease markets – stem cell based regenerative medicine is believed to be the answer
• More than 100 million people suffer from ”western diseases” in the US alone
• Many of these conditions are difficult to cure or even treat with currently available therapies
• Regenerative medicine is believed to be the answer
Medical conditionNumber of US patients (million)
Cardiovascular diseases 70Diabetes 20
Alzheimer’s 5
Parkinson’s 1
Spinal cord injury 0,5
Source: NIH, American Diabetes Association
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Tuesday, August 31, 2010
There is a big need for quality stem cell matrix solutions since many products are of low quality
Stem cells need be grown on a special supporting structure (a growth matrix) in order to keep them alive and expanding
Difficult to grow
The existing matrices have problems with meeting regulatory guidelines (FDA/EMEA), which hinders clinical trials and blocks the approval process for new medicines
Existing solutions are not approved
It is well known that results vary between batches – a concern for both scientists and regulatory officials
Existing solutions are of limited scientific use
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Tuesday, August 31, 2010
We have the solution to researchers problems
Laminins are easy to use
and give reliable data Difficult to grow
Existing solutions are not approved
Existing solutions are of limited scientific use
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Biorelevant and safe
From bench to bedside
Tuesday, August 31, 2010
We have the solution to researchers problems
Laminins are easy to use
and give reliable data Difficult to grow
Existing solutions are not approved
Existing solutions are of limited scientific use
4
Biorelevant and safe
From bench to bedside
Tuesday, August 31, 2010
The science behind our products
Tuesday, August 31, 2010
Basement membrane surrounds most cells and tissues
Blood vessel
Epithelial sheet
Muscle
Basementmembrane
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Tuesday, August 31, 2010
Laminins are the natural matrix for almost all cell types
• family of 15 isoforms
• major component of basal
lamina in vivo
• tissue-specific distribution
• cell adhesion, migration,
proliferation and differentiation
• Impossible to purify from tissues in native forms
α 1-5
β 1-3γ 1-3
Tuesday, August 31, 2010
We do not believe “one size fits all”Neurobiology:
DRG neurons: LN-511
Schwann cells: LN-411
Motoneurons: LN-211
Tooth innervation: LN-411
Pancreas:
Insulin producing beta-cells
LN-511, LN411
Kidney:
Podocytes: LN-521
Stem Cell Biology:
Embryonic stem cells: LN511
Hematopoietic stem cells LN-511
iPS cells: LN511
Immunology:
Neutrophils: LN411
Thymocytes: LN511, LN332, LN221
Muscle:
Cardiac muscle: LN221, LN211
Skeletal muscle: LN211
Skin:
Keratinocytes: LN332
Vascular system:
Vascular endothelial cells: LN511, LN411
Tuesday, August 31, 2010
The ability of a single molecule to promote mES cell growth is unique
On LN-511:
• Stable proliferation rate
> 5 months > 30 passages
On LN-111, LN-411, Matrigel, Gelatin, Poly-D-Lysine:
• Proliferation ceased within two weeks
• ES cells either differentiated or died
LN-511
Matrigel
LN-411
LN-111
GelatinPoly-D-Lys
150
100
50
00 50 100 150
T (days)
N (d
oubl
ings
)
Domogatskaya et al 2008
Tuesday, August 31, 2010
Using multiple laminins in cell differentiation can increase safety
LN-511 LN-411
Matrigel Matrigel
GrowthFactors?
GrowthFactors?
Specific matrixfor specificcells
One size fits all
LN-411 only supports differentiated cellsleading to increased safety
Undifferentiated cell Differentiated cell
Tuesday, August 31, 2010
Three month old mES cells grown on laminin-511 maintained full biological activity
Expand for3 months
mouse blastocyst (line C57Bl black)mouse ES cells (line RW4 brown)
inject
Chimeric mice Germ line transmission
Domogatskaya et al 2008
Tuesday, August 31, 2010
Laminin-511 is the biorelevant glue that keeps the embryo together
Tuesday, August 31, 2010
Laminin-511 is the biorelevant glue that keeps the embryo together
Tuesday, August 31, 2010
Almost all hES cells are pluripotent after 5 months in culture and grow as monolayers
Monolayers !!!
Rodin et al 2010
Tuesday, August 31, 2010
ES cells grow as monolayers most likely because ES cells have a higher affinity for Laminin-511 than any known other matrix
100%
50%
0%LN-511
MG LN-411
LN-111
GEL PL
Domogatskaya et al 2008
Tuesday, August 31, 2010
Advantages with a defined, biorelevant matrix
Tuesday, August 31, 2010
Recombinant laminins can help drive stem cell research forward
1. Defined proteins give reliable and repeatable research results
2. Biological specificity allows to make relevant conclusions
3. Possibility to take the safe conclusions from bench to clinic (human, GMP quality)
Tuesday, August 31, 2010
Imitate the body - Get biorelevant resultsNeurobiology:
DRG neurons: LN-511
Schwann cells: LN-411
Motoneurons: LN-211
Tooth innervation: LN-411
Pancreas:
Insulin producing beta-cells
LN-511, LN411
Kidney:
Podocytes: LN-521
Stem Cell Biology:
Embryonic stem cells: LN511
Hematopoietic stem cells LN-511
iPS cells: LN511
Immunology:
Neutrophils: LN411
Thymocytes: LN511, LN332, LN221
Muscle:
Cardiac muscle: LN221, LN211
Skeletal muscle: LN211
Skin:
Keratinocytes: LN332
Vascular system:
Vascular endothelial cells: LN511, LN411
Tuesday, August 31, 2010
Best support for you,and your cells
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Tuesday, August 31, 2010