biol 101 chp 7: membrane structure and function
DESCRIPTION
This is a lecture presentation for my BIOL 101 General Biology I students on Chapter 7: Membrane Structure and Function. (Campbell Biology, 10th Ed. by Reece et al). Rob Swatski, Associate Professor of Biology, Harrisburg Area Community College - York Campus, York, PA. Email: [email protected] Please visit my website for more anatomy and biology learning resources: http://robswatski.virb.com/TRANSCRIPT
Membrane Structure &
Function
BIOL 101: General Biology I
Chapter 7
Rob Swatski Associate Professor of Biology
HACC – York Campus 1
2
3
Plasma Membrane
Fluid mosaic
Lipids & proteins
Selective permeability
Fibers of extracellular matrix (ECM)
Glyco- protein
Microfilaments of cytoskeleton
Cholesterol
Peripheral proteins
Integral protein
CYTOPLASMIC SIDE OF MEMBRANE
Glyco- lipid EXTRA-
CELLULAR SIDE OF MEMBRANE
Carbo- hydrate
4
5
6
7
Phospholipid Bilayer
Amphipathic
Hydrophilic
Hydrophobic
8
Phospholipids
Cell structure & function
Polar phosphate
head
Nonpolar fatty acid tails
9
10
11
Membrane Models
Sandwich model (Davson & Danielli,
1935): 2 outer protein layers with the
phospholipid bilayer on the inside
Fluid mosaic model (Singer & Nicolson,
1972): proteins dispersed within
phospholipid bilayer
12
13
Freeze-Fracture
Specialized preparation technique
Splits membrane along the middle
of the bilayer
Confirmed fluid mosaic model
Knife
Plasma membrane Cytoplasmic layer
Proteins
Extracellular layer
Inside of extracellular layer Inside of cytoplasmic layer
TECHNIQUE
RESULTS
14
15
16
17
Membrane Fluidity
Flip-flop ( once per month)
Lateral movement (107 times per second)
18
Membrane proteins
Mouse cell
Human cell
Hybrid cell
Mixed proteins after 1 hour
19
20
Membrane Fluidity
Essential for proper functioning
Colder temp. viscous
Unsaturated fatty acids = more fluid
Saturated fatty acids = less fluid
21
22
Cholesterol
Membrane steroid that plays important role in
membrane fluidity
Warm temp. restrains movement
Cool temp. maintains fluidity by preventing
tight packing
Cholesterol in plasma membrane
23
24
Membrane Proteins
Membrane = collage of proteins
Embedded in plasma
membrane
Determine most of membrane’s
specific functions
25
26
Membrane Proteins
Peripheral proteins
Integral proteins
Transmembrane proteins
Alpha helices (nonpolar amino
acids)
Fibers of extracellular matrix (ECM)
Glyco- protein
Microfilaments of cytoskeleton
Cholesterol
Peripheral proteins
Integral protein
CYTOPLASMIC SIDE OF MEMBRANE
Glyco- lipid EXTRA-
CELLULAR SIDE OF MEMBRANE
Carbo- hydrate
27
N-terminus
C-terminus Helix
Trans-membrane protein
28
Major Functions of Membrane Proteins
Transport Enzymatic
activity Signal
transduction Cell-to-cell recognition
Intercellular joining
Attachment to
cytoskeleton &
extracellular matrix (ECM)
29
(a) Transport (b) Enzymatic activity (c) Signal transduction
ATP
Enzymes
Signaling molecule
Receptor
30
31
Sucrose porin (Transport protein)
32
Succinate dehydrogenase
(Enzyme)
33 Cytochrome C Oxidase (Enzyme)
34 G protein (Signal transduction)
(d) Cell-to-cell recognition
Glyco- protein
(e) Intercellular joining (f) Attachment to cytoskeleton & ECM
35
36
HIV ligand receptor binding
(Cell-to-cell recognition)
HIV gp120 ligand
CD4 gp receptor
Antibody protein
37 Adherens junction
38 Bacterial ECM on 1 grain of sand
39
Membrane Carbohydrates
Surface sugars
Cell-to-cell recognition
Glycolipids
Glycoproteins
Variation: species, individual, cell types
Cell membrane
WBC membrane
Receptor (CD4)
Co-receptor (CCR5)
HIV
Receptor (CD4) but no CCR5 Plasma
membrane
HIV can infect a cell that has CCR5 on its surface,
as in most people.
HIV cannot infect a cell lacking CCR5 on its surface, as in
resistant individuals.
40
41
Membrane Sidedness
Outside = extracellular face
Inside = cytoplasmic face
Asymmetrical distribution of proteins,
lipids, & sugars determined by ER &
Golgi
Transmembrane glycoproteins
ER
ER lumen
Glycolipid
Plasma membrane:
Cytoplasmic face
Extracellular face
Secretory protein
Golgi apparatus
Vesicle
Transmembrane glycoprotein Secreted
protein
Membrane glycolipid
42
43
Selective Permeability
Regulates the cell’s molecular traffic
Hydrophobic nonpolar molecules:
dissolve & rapidly move across membrane
[hydrocarbons]
Hydrophilic polar molecules: do not easily move across membrane [sugars]
44
Transport Proteins
Allow passage of specific hydrophilic substances across
membrane
Channel proteins (hydrophilic tunnel for
ions)
Aquaporins (water transport)
Carrier proteins (bind to molecules & change shape)
45
K+ Channel Protein
46
47
Diffusion
Tendency for molecules to spread out evenly
into the available space
Each molecule moves randomly
A population of molecules exhibits a
net movement in one direction
Dynamic equilibrium
48 Diffusion
49
Concentration gradient
50
Passive Transport
Substances diffuse down their
concentration gradient
Move from an area of higher to lower concentration
No energy required = Passive Transport
Net diffusion Net diffusion Equilibrium
51
Diffusion of one solute
Net diffusion
Net diffusion
Net diffusion
Net diffusion
Equilibrium
Equilibrium
52
Diffusion of two solutes
53
Osmosis
Diffusion of water across a selectively
permeable membrane
Water moves from an area of lower to
higher solute concentration
Solutes cannot cross membrane
Low solute concentration
H2O
High solute concentration
Selectively permeable membrane
Equal solute concentration
Osmosis
54 Osmosis
Tonicity: the ability of a surrounding
solution to cause a cell to gain or lose water
Isotonic Hypotonic Hypertonic
55
56
Isotonic
57
Hypotonic
58
Hypertonic
Hypotonic solution
Lysis Normal
Isotonic solution
Shriveled
Hypertonic solution
59
Tonicity in Animal Cells
60
Osmo-regulation
Hypotonic & hypertonic
environments create osmotic problems
for organisms
Osmoregulation = the control of solute
concentrations & water balance
Paramecium & contractile vacuoles
61
Contractile Vacuoles
Hypotonic solution
Turgid (normal, firm)
Isotonic solution
Flaccid (limp) Plasmolysis
Hypertonic solution
62
Tonicity in Plant Cells
63
Facilitated Diffusion
Transport proteins help speed up
passive transport
Ion channels
Gated channels
Aquaporins
Carrier proteins
64
Channel Protein
65
Carrier Protein
66
Active Transport
Requires ATP
Moves substances against their
concentration gradient
Ions, amino acids, glucose
Aided by specific membrane proteins
Sodium-potassium pump
ATP
EXTRACELLULAR
FLUID [Na+] high
[K+] low
Na+
Na+
Na+ [Na+] low
[K+] high CYTOPLASM
Cytoplasmic Na+ binds to the sodium-potassium pump.
1
67
Na+ binding stimulates phosphorylation by ATP.
Na+
Na+
Na+
ATP P
ADP
2
68
Phosphorylation causes the protein to change its shape. Na+ is expelled to the outside.
Na+
P
Na+ Na+
3
69
K+ binds on the extracellular side and triggers release of the phosphate group.
P P
4
70
Loss of the phosphate restores the protein’s original shape.
5
71
K+ is released, and the cycle repeats.
72
73
Electro-chemical Gradient
Voltage: created by differences in
distribution of +/- ions
Membrane potential = voltage
difference
Electrochemical gradient =
combination of chemical &
electrical forces
74
Electrogenic Pumps
Transport proteins that
generate voltage across a
membrane
Help store energy that can be used for cellular work
Sodium-potassium pump (major pump in
animal cells)
Proton pump (major pump in
plants, fungi, bacteria)
H+
H+
H+
H+
H+
H+
+
+
+
H+
+
+
H+
–
–
–
–
ATP
–
75
Proton Pump
76
Cotransport
Active transport of a solute indirectly
drives transport of another solute
Also called coupled transport
Plants use H+ gradient from proton pumps to
actively transport nutrients into cells
Proton pump
–
–
–
–
–
–
+
+
+
+
+
+
ATP
H+
H+
H+ H+
H+
H+
H+
H+
Diffusion of H+ Sucrose-H+
cotransporter
Sucrose
Sucrose
LOW
HIGH
LOW HIGH
77
Bulk Transport: Endocytosis & Exocytosis – requires energy
Phagocytosis (“cell eating”)
Pinocytosis (“cell
drinking”)
Receptor-Mediated
Endocytosis
78
Pseudopodium
Solutes
“Food” or other particle
Food vacuole
CYTOPLASM
EXTRACELLULAR FLUID
Pseudopodium of amoeba
Bacterium
Food vacuole
An amoeba engulfing a bacterium via phagocytosis (TEM).
Phagocytosis
1
m
79
80
Phagocytosis
Pinocytosis vesicles forming in a cell lining a small blood
vessel (TEM).
Plasma membrane
Vesicle
0.5
m
Pinocytosis
81
Top: A coated pit. Bottom: A coated vesicle forming during
receptor-mediated endocytosis (TEMs).
Receptor
0.2
5
m
Receptor-Mediated Endocytosis
Ligand
Coat proteins
Coated pit
Coated vesicle
Coat proteins
Plasma membrane
82
83
Exocytosis
Secretory vesicles
Enzymes, hormones, NT’s,
wastes