BIOINFORMATIK  I ÜBUNGEN

http://icbi.at/bioinf

Regulation of transcription

TF classification by DNA‐binding

A. Zinc fingers B. Helix-turn-helix

C. Leucine zipper D. Helix-loop-helix

• hetero dimerization

• homo dimerization

Transcription factor dimerizationLeucine zippers

Protein 3D‐structure

Example:  PPARg::RXRa+Rosi, 9‐cis RA, NCOA2 (3dzy)

• PDB• JMol• Cn3D• POLYVIEW‐3D• Proteopedia

PDB identification code

Recombinant DNA technology

• Protein synthesis

• In vitro expression

• Libraries

• Gene transfer in animals and plants

Recombinant DNA technology

• Polymerases

• Nucleases

• restriction enzymes

Recombinant DNA technology

Recombinant DNA technology

• Ligases

Cloning

Cloning vector (pBR322)

• Plasmid

• Ampicillin resistance

• Tetracycline resistance

• Polylinker

• Origin of replication (ori)

Selection by antibiotics

Selection by X‐gal

Exercise 3