Bioinformatics Preliminary Lab

1. What does Amylase do?Hint: Give findings from Swissprot, Genecards and KEGGInclude screen snapshots of your findings

2. Obtain the sequences of two (2) yeast amylase proteins.Hint: Use Swissprot at expasyInclude screen snapshots of your findings

3. Give the ENA accession number of AMY2B_HUMAN. Hint:Use EBI ENAInclude screen snapshots of your findings

4. Obtain the AMY2B_HUMAN gene.Hint: Use EMBL, ACcession number from ENAInclude screen snapshots of your findings

5. Give the partial genomic DNA of a mouse and translate it into protein. Compare to partial human protein amylase 2B. Hint: EBI: dbfetchInclude screen snapshots of your findings

6. List two products of Human Recombinant Protein and antibody for AMY2B respectively.Hint:Use Genecards.Include screen snapshots of your findings

7. Predict the protein secondary structures of human and mouse amylaseHint:Expasy: GORInclude screen snapshots of your findings

Relevant webpages: 1. Swissprot at expasy 2. NCBI (ORF Finder)

3. EBI

4. Genecards at DKFZ

5. KEGG

Send your answers in pdf format to eh222.cbe647@gmail.com by Sunday midnight 11.59 pm 21 September 2014. Write your full name and student number (eg. Johnny Depp_2011xxxxxx) as the subject of your email.