Bioinformatics Core (B)Progress and Future Goals

www.functionalglycomics.org

Ensemble or group of glycan structures resulting from coordinated expression of several biosynthetic enzymes

Non-template biosynthesis

Challenges

• Chemical heterogeneity • Challenges in isolation and analysis• Challenges in representation/processing glycan Information

Multivalent interactions involving multiple glycan motifs with multivalent CRDs on proteins

Glycan-Protein InteractionGlycan

Structure-FunctionRelationships

Challenges

• Understanding biochemical basis for glycan-protein interactions• How glycan specific genotype governs whole organism phenotype• Constructing biological pathways and interaction networks

Advancing GlycomicsAdvancing GlycomicsKey Issues & ChallengesKey Issues & Challenges

Consortium for Functional Consortium for Functional GlycomicsGlycomics

www.functionalglycomics.orgwww.functionalglycomics.org

• Active collaborative effort to advance glycomics

• Goal: Understand role of glycan–protein interactions in cell-cell communication

• Develop technologies and resources to obtain data at various levels from molecule mouse

• Integrated approach to glycan structure-function

Bioinformatics Core (B)Bioinformatics Core (B)

Vision & Approach

• Vision: Present the dynamic “face” of the Consortium via Internet to best utilize the value of the resources and datasets generated by its participants and the broader scientific community.

Object-based Relational Database implemented using

Oracle

Middleware implemented using Entity-Class

Operations, Servlets in Java

Static Pages (SHTML) for

Web Site

Dynamic pages (JSPs) for database driven

Entry, Dissemination and Queries

BIOFUNCTION_CATEGORY

Biofunction_key: INTEGER

Biofunction_name: VARCHAR2(20)

BIOLOGICAL_SOURCE

biosource_key: INTEGER

Biosource_ID: VARCHAR2(20)kingdom: VARCHAR2(20)Phylum: VARCHAR2(20)Class: VARCHAR2(20)Genus: VARCHAR2(20)Species: VARCHAR2(20)

CARBOHYDRATE

Carb_key: INTEGER

biosource_key: INTEGERCarb_ID: VARCHAR2(20)Carb_Sciname: VARCHAR2(20)Carb_commonName: VARCHAR2(20)Carb_Family: VARCHAR2(20)Carb_Subfamily: VARCHAR2(20)Carb_Type: VARCHAR2(10)Carb_MW: NUMBERBiofunction_key: NUMBERCarb_BiofunctionDesc: VARCHAR2(100)Carb_listdate: DATECarb_structure: VARCHAR2(500)Carb_structureImgtype: VARCHAR2(10)Carb_structureImg: BLOBAccess_code: VARCHAR2(20)

DNASEQ

DNASEQ_key: INTEGER

Gene_key: INTEGERDNASEQ_ID: VARCHAR2(20)DNASEQ_IDtype: VARCHAR2(20)DNASEQ_sequence: VARCHAR2(20)

GENE

Gene_key: INTEGER

Genome: VARCHAR2(20)Gene_name: VARCHAR2(20)biosource_key: INTEGER

PAPER

Paper_key: NUMBER

Paper_ID: VARCHAR2(20)ID_type: VARCHAR2(20)Paper_title: VARCHAR2(200)Paper_year: INTEGERJournal_name: VARCHAR2(200)Page_range: VARCHAR2(20)

PAPER_REFERENCE

Paper_key: NUMBERCarb_key: INTEGER

PROTEIN

Protein_key: INTEGER

Protein_ID: VARCHAR2(20)ProteinID_type: VARCHAR2(20)Protein_name: VARCHAR2(20)Protein_CommonName: VARCHAR2(20)Protein_family: VARCHAR2(20)Protein_subFamily: VARCHAR2(20)Protein_superUnitKey: INTEGERProtein_type: VARCHAR2(20)Biosource_key: INTEGERBiofunction_key: INTEGERGene_key: VARCHAR2(20)DNASEQ_key: INTEGERProtein_sequenceKey: INTEGERProtein_biofunctionDesc: VARCHAR2(20)Access_code: VARCHAR2(20)Protein_listdate: DATE

PROTEINSEQUENCE

Protein_sequenceKey: INTEGER

Protein_sequence: VARCHAR2(20)Protein_key: INTEGER

REAGENT

Reagent_key: INTEGER

Reagent_ID: VARCHAR2(20)Reagent_IDtype: VARCHAR2(20)Reagnet_name: VARCHAR2(20)Reagent_formula: VARCHAR2(20)Reagent_MW: INTEGER

CPBINDING

Cpb_key: NUMBER

Cpb_ID: VARCHAR2(20)Cpb_IDtype: VARCHAR2(20)Carb_key: NUMBERProtein_key: NUMBERCpb_flag: CHAR(1)Biofunction_key: NUMBERCpb_BiofunctionDesc: VARCHAR2(100)Cpb_Keq: NUMBERCpb_DG: NUMBERCpb_DH: NUMBERCpb_DS: NUMBERCpb_listdate: DATE

PROTOCOL

Protocol_key: INTEGER

Protocol_ID: VARCHAR2(20)Protocol_Category: VARCHAR2(20)Protocol_keyword: VARCHAR2(20)Protocol_Title: VARCHAR2(20)Protocol_overview: VARCHAR2(20)Protocol_procedure: CHAR(18)Protocol_trobleshooting: VARCHAR2(20)Dbuser_key: INTEGER

CPATHREAGENT

Protocol_key: INTEGERReagent_key: INTEGER

Reactant_Weightg: INTEGERReactant_mmol: VARCHAR2(20)

CPATHINSTRUMENT

Protocol_key: INTEGERInstrument_key: INTEGER

INSTRUMENT

Instrument_key: INTEGER

Instrument_category: VARCHAR2(20)Instrument_name: VARCHAR2(20)Instrument_model: VARCHAR2(20)Instrument_Vender: VARCHAR2(20)

CARBPROFILE

Carbprofile_key: NUMBER

Carbprofile_ID: VARCHAR2(20)Carbprofile_title: VARCHAR2(30)Carbprofile_carbComp: VARCHAR2(200)Carbprofile_avgMW: NUMBERMouseind_key: NUMBERCarbprofile_source: VARCHAR2(100)Biofunction_key: NUMBERCarbprofile_biofunctionDesc: VARCHAR2(200)Parentprofile_ID: VARCHAR2(20) CPROFILEID

Carbprofile_key: NUMBERCarb_key: INTEGER

Concentration: VARCHAR2(20)CPROFILEEXP

Cpexp_key: INTEGER

cpexp_ID: VARCHAR2(20)cpexp_title: VARCHAR2(20)Protocol_key: INTEGERCarbprofile_key: NUMBERCpexp_keyword: VARCHAR2(20)Cpexp_instrument: VARCHAR2(20)Cpexp_date: DATECpexp_comment: VARCHAR2(20)Dbuser_key: INTEGER

MICROARRAY

Marray_key: INTEGER

Marray_ID: VARCHAR2(20)Marray_type: VARCHAR2(20)Marray_desc: VARCHAR2(20)Marray_imgtype: VARCHAR2(20)Marray_img: BLOB

MOUSESTRAIN

Mouse_key: INTEGER

Mouse_ID: VARCHAR2(20)Cell_key: NUMBERMouse_type: VARCHAR2(20)Protocol_key: INTEGERMouse_keyword: VARCHAR2(20)Mouse_desc: VARCHAR2(20)

KOGENE

KOgene_key: INTEGER

KOgene_ID: VARCHAR2(20)Mouse_key: INTEGERGene_key: INTEGERGene_ID: VARCHAR2(20)KOgene_synonym: VARCHAR2(20)KOgene_Mtype: VARCHAR2(20)KOgene_UnigenDes: VARCHAR2(20)KOgene_HumanGene: VARCHAR2(20)

CELLSOURCE

Cell_key: NUMBER

Cell_ID: VARCHAR2(20)biosource_key: NUMBERCell_line: VARCHAR2(30)Cell_linetype: VARCHAR2(20)Cell_tissue: VARCHAR2(20)Cell_Organ: VARCHAR2(20)

MAEXP

Maexp_key: INTEGER

Maexp_ID: VARCHAR2(20)Maexp_title: VARCHAR2(20)Protocol_key: INTEGERMarray_key: INTEGERMaexp_SampleID: VARCHAR2(20)Maexp_SampleDesc: VARCHAR2(20)Maexp_keyword: VARCHAR2(20)Maexp_readtype: VARCHAR2(20)Maexp_raw: VARCHAR2(20)Maexp_date: DATEMaexp_imgfiletype: VARCHAR2(20)Maexp_imgraw: BLOBDbuser_key: INTEGER

TARGETGENE

Maexp_key: INTEGERGene_key: INTEGER

Targetgene_desc: VARCHAR2(20)

PROTIENREF

Paper_key: NUMBERProtein_key: INTEGER

CARBPATHREF

Paper_key: NUMBERCpath_key: INTEGER

PROTOCOLREF

Paper_key: NUMBERProtocol_key: INTEGER

CPBREF

Paper_key: NUMBERCpb_key: NUMBER

DBUSER

Dbuser_key: INTEGER

Dbuser_username: VARCHAR2(20)Dbuser_password: VARCHAR2(20)Dbuser_lastname: VARCHAR2(20)Dbuser_firstname: CHAR(18)Dbuser_email: VARCHAR2(20)Dbuser_employer: VARCHAR2(20)Dbuser_title: VARCHAR2(20)Dbuser_Core: VARCHAR2(20)Dbuser_listdate: DATE

MOUSEHEMA

MH_key: INTEGER

MH_ID: VARCHAR2(20)Mouseind_key: INTEGERMH_date: DATEMH_age: INTEGERMH_weight: INTEGERMH_WBC: INTEGERMH_Neutrophils: INTEGERMH_Lymphocytes: VARCHAR2(20)MH_HGB: INTEGERMH_HCT: INTEGERMH_PLT: INTEGERMH_MPV: INTEGERDbuser_key: INTEGER

MOUSEHISTOLOGY

MHS_key: INTEGER

MHS_ID: VARCHAR2(20)Mouseind_key: INTEGERMHS_date: DATEMHS_age: INTEGERMHS_weight: INTEGERProtocol_key: INTEGERMHS_purpose: VARCHAR2(20)MHS_result: VARCHAR2(20)MHS_comment: VARCHAR2(20)Dbuser_key: INTEGER

MOUSEIMMU

MI_key: INTEGER

MI_ID: VARCHAR2(20)Mouseind_key: INTEGERMI_date: DATEMI_age: INTEGERMI_weight: INTEGERMI_sampletype: VARCHAR2(20)MI_CD4: INTEGERMI_CD8: INTEGERB220: INTEGERIAB: INTEGERIgM: INTEGERIgD: INTEGERDbuser_key: INTEGER

MOUSEIND

Mouseind_key: INTEGER

MIND_ID: VARCHAR2(20)Mouse_key: INTEGERMind_sex: VARCHAR2(20)

MOUSEBEHAV

MB_key: INTEGER

MB_ID: VARCHAR2(20)Mouseind_key: INTEGERMB_date: DATEMB_age: INTEGERMB_weight: INTEGERMB_GrossNero: VARCHAR2(20)MB_SensorMotorReflex: VARCHAR2(20)MB_PosturalReflex: VARCHAR2(20)MB_motoractivity: VARCHAR2(20)MB_Nocieption: VARCHAR2(20)MB_AcousticStartle: VARCHAR2(20)MB_SocialDomine: VARCHAR2(20)MB_conditionedFear: VARCHAR2(20)MB_waterMaze: VARCHAR2(20)MB_radialArmMaze: VARCHAR2(20)Dbuser_key: INTEGER

MOUSEMETABOLISM

MM_key: INTEGER

MM_ID: VARCHAR2(20)Mouseind_key: CHAR(18)MM_date: DATEMM_age: INTEGERMM_weight: INTEGERMM_Cardiac: VARCHAR2(20)MM_BloodDiastol: INTEGERMM_bloodSystol: INTEGERMM_plumonary: VARCHAR2(20)MM_FoodConsumption: INTEGERMM_waterConsumption: INTEGERMM_activityLevel: INTEGERMM_OxygenConsumption: INTEGERMM_CO2Output: INTEGERMM_HeatOutput: INTEGERDbuser_key: INTEGER

MAREF

Marray_key: INTEGERPaper_key: NUMBER

ENZYME

Enzyme_key: INTEGER

Enzyme_ID: VARCHAR2(20)Enzyme_type: VARCHAR2(20)Enzyme_name: VARCHAR2(20)Enzyme_CommonName: VARCHAR2(20)Enzyme_family: VARCHAR2(20)Enzyme_subFamily: VARCHAR2(20)biosource_key: INTEGERBiofunction_key: INTEGEREnzyme_biofunctionDesc: VARCHAR2(20)Gene_key: VARCHAR2(20)DNASEQ_key: INTEGEREnzyme_sequenceKey: INTEGERProteindb_ID: VARCHAR2(20)SwissPort_ID: VARCHAR2(20)Access_Code: VARCHAR2(20)Protein_listdate: DATE

CARBPATHWAY

Cpath_key: INTEGER

Cpath_name: VARCHAR2(20)Carb_key: INTEGEREnzyme_key: INTEGERCpath__Reaction: VARCHAR2(20)Cpath_condition: VARCHAR2(20)Cpath_stepFromCarb: INTEGERCpath_yield: INTEGER

ENZYMESEQUENCE

Enzyme_sequenceKey: INTEGER

Enzyme_sequence: VARCHAR2(20)Enzyme_key: INTEGER

CARBEXP

Carbexp_key: INTEGER

carbexp_ID: VARCHAR2(20)carbexp_title: VARCHAR2(30)Protocol_key: NUMBERCarb_key: NUMBERCarbexp_keyword: VARCHAR2(100)Carbexp_Instrument: VARCHAR2(100)Carbexp_comment: VARCHAR2(200)Carbexp_date: DATECabexp_batchno: VARCHAR2(20)User_key: NUMBER

CARBEXPIMG

carbExpImg_key: INTEGER

Carbexp_key: NUMBERCarbExpImg_exptype: VARCHAR2(20)CarbExpImg_filetype: VARCHAR2(20)CarbExpImg_imageRaw: BLOB

CPBEXP

Cpbexp_key: NUMBER

cpbexp_ID: VARCHAR2(20)cpbexp_title: VARCHAR2(20)Protocol_key: INTEGERCpb_key: NUMBERCpbexp__keyword: VARCHAR2(20)Cpbexp_Instrument: VARCHAR2(20)Cpbexp_date: DATECpbexp_comment: VARCHAR2(20)Dbuser_key: INTEGER

CPBEXPIMG

cpbExpImg_key: INTEGER

Cpbexp_key: NUMBERCpbexpImg_exptype: VARCHAR2(20)CpbexpImg_imageRaw: BLOBCpbexpimg_desc: VARCHAR2(20)CpbexpImg_filetype: VARCHAR2(20)

CPROFILEEXPIMG

cpexpImg_key: INTEGER

Cpexp_key: INTEGERCpexpImg_exptype: VARCHAR2(20)CpexpImg_filetype: VARCHAR2(20)CpexpImg_imageRaw: BLOBCpexpImg_desc: VARCHAR2(20)

MAEXPANALYSIS

Maexpanaly_key: INTEGER

Maexp_key: INTEGERMaexpanaly_title: VARCHAR2(100)Maexpanaly_desc: VARCHAR2(500)

CBPEXP

Cbpexp_key: INTEGER

Cbpexp_ID: VARCHAR2(20)Cbpexp_title: VARCHAR2(20)Protocol_key: INTEGERProtein_key: INTEGERCbpexp_keyword: VARCHAR2(20)Cbpexp_Instrument: VARCHAR2(20)Cbpexp_comment: VARCHAR2(20)Cbpexp_date: DATEDbuser_key: INTEGER

ENZEXPIMG

EnzexpImg_key: INTEGER

Enzexp_key: INTEGEREnzexpImg_exptype: VARCHAR2(20)EnzexpImg_filetype: VARCHAR2(20)EnzexpImg_imageRaw: BLOB

ENZEEXP

Enzexp_key: INTEGER

Enzexp_ID: VARCHAR2(20)Enzexp_title: VARCHAR2(20)Protocol_key: INTEGEREnzyme_key: INTEGEREnzexp_keyword: VARCHAR2(20)Enzexp_Instrument: VARCHAR2(20)Enzexp_comment: VARCHAR2(20)Enzexp_date: DATEUser_key: INTEGER

CBPEXPIMG

CbpexpImg_key: INTEGER

Cbpexp_key: INTEGERCbpexpImg_exptype: VARCHAR2(20)CbpexpImg_filetype: VARCHAR2(20)CbpexpImg_imageRaw: BLOB

GLYCOARRAY

Garray_key: INTEGER

Garray_ID: VARCHAR2(20)Garray_type: VARCHAR2(20)Garray_desc: VARCHAR2(20)Garray_imgtype: VARCHAR2(20)Garray_img: BLOB

GAEXP

Gaexp_key: INTEGER

Gaexp_ID: VARCHAR2(20)Gaexp_title: VARCHAR2(20)Protocol_key: INTEGERGarray_key: INTEGERGaexp_SampleID: VARCHAR2(20)Gaexp_SampleDesc: VARCHAR2(20)Gaexp_keyword: VARCHAR2(20)Gaexp_readtype: VARCHAR2(20)Gaexp_raw: VARCHAR2(20)Gaexp_date: DATEGaexp_imgfiletype: VARCHAR2(20)Gaexp_imgraw: BLOBDbuser_key: INTEGER

TARGETPROTEIN

Gaexp_key: INTEGERProtein_key: INTEGER

Targetcbp_desc: VARCHAR2(20)

CPATHEXP

Cpathexp_key: INTEGER

Cpathexp_ID: VARCHAR2(20)Cpathexp_title: VARCHAR2(20)Cpath_key: INTEGERProtocol_key: INTEGERCpathexp_keyword: VARCHAR2(20)Cpathexp_Instrument: VARCHAR2(20)Cpathexp_comment: VARCHAR2(20)Cpathexp_date: DATEUser_key: INTEGER

CPATHEXPIMG

CpathexpImg_key: INTEGER

Cpathexp_key: INTEGERCpathexpImg_exptype: VARCHAR2(20)CpathexpImg_filetype: VARCHAR2(20)CpathexpImg_imageRaw: BLOB

CARBINVENTORY

Carbinv_key: NUMBER

Carbinv_ID: VARCHAR2(20)Carb_key: NUMBERCarbexp_key: VARCHAR2(20)Carbinv_Purity: NUMBERCarbinv_color: VARCHAR2(10)Carbinv_State: VARCHAR2(10)Quantity: NUMBERCarbexp_batchno: VARCHAR2(20)

CARBORDER

Corder_key: NUMBER

Corder_ID: VARCHAR2(20)Corder_date: DATECarbinv_key: NUMBEROrder_receiver: VARCHAR2(50)Order_address: VARCHAR2(200)Order_quantity: NUMBERshipping_flag: CHAR(1)

Part of CFG database overall ontology map

Core B OrganizationCore B Organization

Core Operations &

Management

BioinformaticsScientific liaisons, User specifications,

Bioinformatics applications

Information TechnologyDatabase, web, software

applications, user interface development

AdministrationRam Sasisekharan (Core Coordinator), Rahul Raman (Core Director)

Ada Ziolkowski (Admin Staff)

Core B Team Maha Venkataraman, Subu Ramakrishnan, Savitri Subramanian, Thomas Lutteke

Ganesh Venkataraman, Wei Lang, Eric Berry, Nishla Keiser, Ishan Capila, Chipong Kwan and Consultants

MIT Information Systems

Server Maintenance

MIT Information Systems

Database Administration

Consortium DB

Data Objects, Relationships, Raw and Parsable Data

Public Databases

GBP, Glycans, Glycoenzymes

User InterfaceData Acquisition

Web-based Forms Annotation Tools

User Interface for InformationDissemination

GBP Molecule

Pages

GlycoEnzyme Molecule

Pages

Glycan StructuresDatabase

Consortium Data

Dissemination

Implementation StrategyImplementation StrategyDatabases and Interfaces

Implementation StrategyImplementation StrategySchema of CFG Data Objects and Integration

Glycobiology, 16(5), 82R-90R

Highlights of ProgressHighlights of ProgressYears 1-4

• Infrastructure set-up

• Database implementation

• Software development

• Interactions with Cores for data acquisition and dissemination

Years 1 - 2• Acquisition of Core data

• Public release of databases

• CFG data dissemination

• GBP molecule pages

• Glycan structures DB

Years 3-4

Highlights of ProgressHighlights of ProgressQuarters 1-3 of Year 5

CFG data acquisition and dissemination• Streamlining the process to upload printed array data from Cores D and H• Enhancing interfaces for disseminating scientific Core data• Implementation of data dissemination and research tracking interfaces for PIs

Specialized databases and molecule pages• Data organization and implementation of glycosyltransferase database• Updating expert contribution fields for C-type lectins in GBP database

Other key highlights• Bioinformatics satellite session at the Society for Glycobiology 2005 meeting –

discussion of glycan analysis methods and data exchange formats• Renewal application and preparation for Council review

Publications• “Glycomics: An integrated systems approach to structure-function relationships of glycans.”

Nature Methods, 2(11): 817-24• “Advancing glycomics: implementation strategies at the consortium for functional glycomics.”

Glycobiology, 16(5): 82R-90R

CFG Scientific Core DataCFG Scientific Core DataData Organization

Experiment Information

Brief description of scope of experiment or analysis

Experiment Information

Brief description of scope of experiment or analysis

Protocols

Standardized Protocols used in an experiment or

analysis

Protocols

Standardized Protocols used in an experiment or

analysis

Sample/Resources

Detailed information on sample / resource utilized [mouse, tissue, cell, GBP]

Sample/Resources

Detailed information on sample / resource utilized [mouse, tissue, cell, GBP]

Data Summary

Summary of interpretation of data by Scientific Core

Data Summary

Summary of interpretation of data by Scientific Core

Raw Data

Raw data files generated by the analysis – Images, Excel,

binary formats

Raw Data

Raw data files generated by the analysis – Images, Excel,

binary formats

Annotated Data

Processed information on entities such as gene, mouse, GBP, etc. stored in DB tables

Annotated Data

Processed information on entities such as gene, mouse, GBP, etc. stored in DB tables

CFG Scientific Core DataCFG Scientific Core Data Statistics (as of June 2006)

Gene Microarray (E)

47 Experiments (33)

617 Samples (387)

3546 data files (1826)

Mouse Phenotyping (G)

16 KO Strains (11)

266 Experiments (149)

3116 data files (1100)

Glycotechnology (C)

Mouse: 11 tissues, 8 KO strains, 92 MALDI-MS spectra

Human: 11 Tissues 108 MALDI-MS spectra

Cell Lines: 12 Cell Lines 27 Spectra

GBP-Glycan (H)

247 Samples (143)

CFG Scientific Core DataCFG Scientific Core DataEnhanced dissemination interfaces

Navigation and downloading of CFG Data – Gene Expression, MALDI-MS Glycan Profiling, Mouse Phenotyping and Glycan Array - DEMO

Gene Microarray DataGene Microarray Data

Gene Microarray DataGene Microarray Data

Gene Microarray DataGene Microarray Data

Mouse Phenotyping DataMouse Phenotyping Data

Mouse Phenotyping DataMouse Phenotyping Data

Mouse Phenotyping DataMouse Phenotyping Data

Mouse Phenotyping ProtocolsMouse Phenotyping Protocols

Glycan Array DataGlycan Array Data

Glycan Array DataGlycan Array Data

Glycan Array DataGlycan Array Data

Glycan Array DataGlycan Array Data

CFG Data IntegrationCFG Data IntegrationExpression of glycan related genes in wildtype mouse spleen

CFG Data IntegrationCFG Data IntegrationGlycan profile of spleen of FucT-VII KO mice with differences in histology staining

Tracking PI Research ProgressTracking PI Research ProgressData dissemination and resource tracking

interface

PI

Core A

Database

Request ID Data

IDs

Core

Submit Online Request Form

Copy of submitted request emailed with Request ID in database

SC

Post Status

Prioritizing/ Approved

Request

Approval Process

Upload Datawith Request ID

Tracking PI Research Tracking PI Research ProgressProgress

Data dissemination and resource tracking interface

Navigating PI information page to find associated resource requests and data - DEMO

Automated Acquisition

Data from public databases, links to public resources

Data from Cores

Interface to CFG resources and data

Expert Contribution

Filling out fields as experts on the molecule

MoleculeInformation

Portal

GBP Molecule Page GBP Molecule Page InterfacesInterfaces

Three main componentsThree main components

• Glycan array data

• Mouse phenotyping data

• Transgenic mouse line

Contribution from experts obtained for C-type lectins

GBP Molecule PagesGBP Molecule PagesUpdating expert contributions for C-type Updating expert contributions for C-type

lectinslectins

GBP Molecule Page example for C-type lectins with filled expert contribution fields - DEMO

GBP Molecule PagesGBP Molecule Pages

DC-SIGN Molecule PageDC-SIGN Molecule Page

ExpertContributions

DC-SIGN Molecule PageDC-SIGN Molecule Page

DC-SIGN Molecule PageDC-SIGN Molecule Page

DC-SIGN Molecule PageDC-SIGN Molecule Page

DC-SIGN Molecule PageDC-SIGN Molecule Page

PDB IDs of DC-SIGNcomplexed with glycanligands identified usingPDB2LINUCS tool

ExpertContributions

DC-SIGN Molecule PageDC-SIGN Molecule Page

GT Database DevelopmentGT Database DevelopmentExpert annotation of glycosylation Expert annotation of glycosylation

pathwayspathwaysType II Extension/ TerminationComposite Structure

FucTs expert annotation

343 Genes

40 Sialyl-T24 Fucosyl-T69 GlcNAc-T 47 Gal-T 54 GalNAc-T 78 Sulfo-T 31 Man-T

Automated Acquisition

Data from public databases, links to public resources

Data from Cores

Interface to CFG resources and data

MoleculeInformation

Portal

GT Database DevelopmentGT Database DevelopmentGlycosylation interface & GT molecule Glycosylation interface & GT molecule

pagespages

• Glycan profiling of GT KO mice

• Mouse phenotyping data

• Transgenic mouse line

• Gene expression (Year 6 goals)

Glycan Structures DatabaseGlycan Structures Database

Glycan Structure Search Glycan Structure Search InterfaceInterface

Glycan Structure Search Glycan Structure Search InterfaceInterface

Glycan Structure Search Glycan Structure Search InterfaceInterface

Glycan Structure Search Glycan Structure Search InterfaceInterface

Glycan DB Search ResultsGlycan DB Search Results

Glycan DB Search ResultsGlycan DB Search Results